SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011384093.1 NCBI__GCF_000009985.1:WP_011384093.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6g4dB Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp (see paper)
56% identity, 98% coverage: 9:459/459 of query aligns to 2:452/453 of 6g4dB

query
sites
6g4dB

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active site: Y15 (= Y22), Y146 (= Y153), D253 (= D260), K282 (= K289)
binding pyridoxal-5'-phosphate: S112 (= S119), G113 (= G120), S114 (= S121), N117 (= N124), Y146 (= Y153), H147 (= H154), G148 (= G155), E220 (= E227), D253 (= D260), V255 (= V262), I256 (= I263), K282 (= K289), F318 (≠ Y325), T319 (= T326)

6g4fA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with pmp (see paper)
56% identity, 98% coverage: 9:457/459 of query aligns to 2:450/451 of 6g4fA

query
sites
6g4fA

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active site: Y15 (= Y22), Y146 (= Y153), D253 (= D260), K282 (= K289)
binding pyridoxal-5'-phosphate: F318 (≠ Y325), T319 (= T326)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S112 (= S119), G113 (= G120), S114 (= S121), N117 (= N124), Y146 (= Y153), H147 (= H154), G148 (= G155), E220 (= E227), D253 (= D260), V255 (= V262), I256 (= I263), K282 (= K289)

6g4eA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp and 6-aminohexanoate (6-aca) (see paper)
56% identity, 98% coverage: 9:457/459 of query aligns to 2:450/451 of 6g4eA

query
sites
6g4eA

T

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R

E

D

S

I

L

L

V

A

E

Q

H

V

A

A

A

E

Q

V

M

G

G

P

Q

V

L

L

L

Q

R

D

S

G

G

L

L

R

Q

A

H

L

F

R

I

G

D

H

H

P

P

L

L

V

V

G

G

E

E

A

I

R

R

G

G

V

C

G

G

L

L

I

I

G

A

A

A

V

V

E

E

L

L

V

V

A

G

D

D

K

R

A

V

N

S

R

K

A

A

P

P

F

Y

P

Q

P

A

E

L

L

G

A

T

V

L

G

G

A

R

R

Y

V

M

V

A

A

G

K

R

A

A

Q

Q

A

E

K

H

G

G

V

M

I

I

L

T

R
|
R

A

A

M
|
M

G

G

D

D

A

A

V

V

A

A

F

F

A

C

P

P

P

P

L

L

V

I

I

V

S

N

K

E

A

Q

D

E

I

V

A

G

E

M

M

I

L

V

R

E

R

R

F

F

G

A

L

R

A

A

L

L

D

D

E

D

A

T

H

T

G

Q

E

W

L

V

G

G

active site: Y15 (= Y22), Y146 (= Y153), D253 (= D260), K282 (= K289)
binding 6-aminohexanoic acid: Y15 (= Y22), W53 (= W60), Y146 (= Y153), K282 (= K289), R412 (= R419), M414 (= M421)
binding pyridoxal-5'-phosphate: S112 (= S119), G113 (= G120), S114 (= S121), N117 (= N124), Y146 (= Y153), H147 (= H154), G148 (= G155), E220 (= E227), D253 (= D260), V255 (= V262), I256 (= I263), K282 (= K289), F318 (≠ Y325), T319 (= T326)

5ghgB Transaminase w58l with smba
54% identity, 98% coverage: 9:458/459 of query aligns to 3:433/433 of 5ghgB

query
sites
5ghgB

T

S

I

L

A

E

E

A

K

R

D

D

V

I

D

R

S

Y

V

H

L

L

H

H

P

S

Y
|
Y

T

T

N

D

L

A

K

V

R

R

H

L

P

E

E

A

V

E

G

G

P

P

L

L

V

V

I

I

T

E

R

R

G

G

Q

D

G

G

V

I

R

Y

V

V

Y

E

D

D

E

V

A

S

G

G

R

K

D

R

Y

Y

I

I

E

E

G

A

L

M

A

S

G

G

L

L

W

L

C

S

T

V

S

G

L

V

G

G

W

F

G

S

E

E

E

P

R

R

L

L

V

A

E

E

A

A

A

A

A

A

R

R

Q

Q

M

M

R

K

Q

K

L

L

P

P

F

F

Y

Y

H

H

L

T

F

F

S

-

H

-

K

-

T

-

H

H

D

G

V

P

G

V

V

I

E

D

L

L

C

A

A

E

R

K

L

L

L

V

A

S

M

M

A

A

P

P

V

V

P

P

M

M

S

S

K

K

V

A

F

Y

L

F

A

T

G

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N
|
N

D

D

T

T

A

V

I

V

K

K

L

L

I

I

H

W

Y

Y

R

R

A

S

N

N

A

A

L

L

G

G

T

E

P

P

D

E

K

R

K

K

K

K

I

I

I

I

A

S

R

R

E

K

K

R

A

G

Y
|
Y

H
|
H

G
|
G

V

V

T

T

V

I

A

A

T

S

A

A

S

S

L

L

T

T

G

G

L

L

V

P

N

N

N

N

Q

H

R

R

S

S

F

F

D

D

L

L

P

P

I

I

P

D

G

R

V

I

L

L

R

H

A

T

A

G

C

C

P

P

H

H

H

F

Y

Y

R

R

F

E

A

G

K

Q

D

A

G

G

E

E

S

S

E

E

E

E

D

Q

F

F

S

A

T

T

R

R

L

L

A

A

G

D

E

E

L

L

E

E

A

Q

M

L

I

I

L

I

A

A

E

E

G

G

P

P

D

H

T

T

V

I

A

A

A

A

F

F

F

I

A

G

E
|
E

P

P

V

V

M

M

G

G

A

A

G

G

G

G

V

V

I

V

V

V

P

P

P

P

A

K

G

T

Y

Y

F

W

P

E

K

K

I

V

Q

Q

A

A

V

V

L

L

D

K

R

R

Y

Y

D

D

V

I

L

L

L

L

V

I

V

A

D
|
D

E

E

V
|
V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

K

N

M

L

F

F

G

G

T

S

E

Q

T

T

F

F

G

D

I

M

R

K

P

P

D

D

M

I

M

L

T

V

L

M

A

S

K
|
K

G

Q

L

L

S

S

S

S

G

S

Y

Y

A

L

P

P

I

I

S

S

A

A

L

F

M

L

V

I

N

N

E

E

R

R

V

V

Y

Y

G

A

P

P

V

I

A

A

E

-

E

-

S

-

G

-

R

-

I

-

G

-

V

-

F

-

G

-

H

-

G

-

Y

-

T

-

Y

-

G

S

G

G

H

H

P

P

V

V

S

A

A

A

A

A

V

V

A

A

L

L

E

E

T

N

L

L

N

A

I

I

Y

I

A

E

E

E

R

R

D

D

I

L

L

V

A

A

Q

N

V

A

A

R

E

D

V

R

G

G

P

T

V

Y

L

M

Q

Q

D

K

G

R

L

L

R

R

A

E

L

L

R

Q

G

D

H

H

P

P

L

L

V

V

G

G

E

E

A

V

R

R

G

G

V

V

G

G

L

L

I

I

G

A

A

G

V

V

E

E

L

L

V

V

A

T

D

D

K

K

A

Q

N

A

R

K

A

T

P

G

F

L

P

E

P

P

E

T

L

G

A

A

V

L

G

G

A

A

R

K

V

A

V

N

A

A

K

V

A

L

Q

Q

A

E

K

R

G

G

V

V

I

I

L

S

R

R

A

A

M

M

G

G

D

D

A

T

V

L

A
|
A

F

F

A

C

P

P

P

P

L

L

V

I

I

I

S

N

K

D

A

Q

D

Q

I

V

A

D

E

T

M

M

L

V

R

S

R

A

F

L

G

E

L

A

A

T

L

L

D

N

E

D

A

V

H

Q

G

A

E

S

L

L

G

T

R

R

active site: Y16 (= Y22), Y143 (= Y153), E217 (= E227), D250 (= D260), I253 (= I263), K279 (= K289), A401 (= A426)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S109 (= S119), G110 (= G120), S111 (= S121), N114 (= N124), Y143 (= Y153), H144 (= H154), G145 (= G155), E217 (= E227), D250 (= D260), V252 (= V262), I253 (= I263), K279 (= K289)

Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
50% identity, 97% coverage: 7:449/459 of query aligns to 45:488/504 of Q94CE5

query
sites
Q94CE5

N

K

R

N

T

T

I

V

A

G

E

S

K

K

D

G

V

H

D

D

S

-

V

M

L

L

H

A

P

P

Y

F

T

T

N

A

L

G

K

W

R

Q

H

S

P

A

E

D

V

L

G

D

P

P

L

L

V

V

I

I

T

A

R

K

G

S

Q

E

G

G

V

S

R

Y

V

V

Y

Y

D

D

E

D

A

T

G

G

R

K

D

K

Y

Y

I

L

E

D

G

S

L

L

A

A

G

G

L

L

W

W

C
|
C

T

T

S

A

L

L

G

G

W
x
G

G

N

E

E

E

P

R

R

L

L

V

V

E

S

A

A

A

A

A

V

R

E

Q

Q

M

L

R

N

Q

T

L

L

P

P

F

F

Y

Y

H

H

L

S

F

F

S

W

H

N

K

R

T

T

H

T

D

K

V

P

G

S

V

L

E

D

L

L

C

A

A

K

R

V

L

L

L

L

A

E

M

M

A

F

P

T

V

A

-

N

P

K

M

M

S

A

K

K

V

A

F

F

L

F

A

T

G

S

S

G

G

G

S

S

E

D

A

A

N

N

D

D

T

T

A

Q

I

V

K

K

L

L

I

V

H

W

Y

Y

R

Y

A

N

N

N

A

A

L

L

G

G

T

R

P

P

D

E

K

K

K

K

K

K

I

F

I

I

A

A

R

R

E

K

K

K

A

S

Y

Y

H

H

G

G

V

S

T

T

V

L

A

I

T

S

A

A

S

S

L

L

T

S

G

G

L

L

V

P

N

P

N

L

Q

H

R

Q

S

N

F

F

D

D

L

L

P

P

I

A

P

P

G

F

V

V

L

L

R

H

A

T

A

D

C

C

P

P

H

H

H

Y

Y

W

R

R

F

F

A

H

K

L

D

P

G

G

E

E

S

T

E

E

E

E

D

E

F

F

S

S

T

T

R

R

L

L

A

A

G

K

E

N

L

L

E

E

A

D

M

L

I

I

L

I

A

K

E

E

G

G

P

P

D

E

T

T

V

I

A

G

A

A

F

F

F

I

A

A

E

E

P

P

V

V

M

M

G

G

A

A

G
|
G

G

G

V

V

I

I

V

P

P

P

P

P

A

A

G

T

Y

Y

F

F

P

E

K

K

I

V

Q

Q

A

A

V

V

L

V

D

K

R

K

Y

Y

D

D

V

I

L

L

L

F

V

I

V

A

D

D

E

E

V

V

I

I

C

C

G

A

F

F

G

G

R

R

T

L

G

G

K

T

M

M

F

F

G
|
G

T

C

E

D

T

K

F

Y

G

N

I

I

R

K

P

P

D

D

M

L

M

V

T

T

L

L

A

A

K

K

G

A

L

L

S

S

S

S

G

A

Y

Y

A

M

P

P

I

I

S

G

A

A

L

I

M

L

V

M

N

S

E

Q

R

E

V

V

Y

A

G

D

P

V

V

I

A

N

E

S

E

H

S

S

G

S

R

K

I

L

G

G

V

V

F

F

G

S

H

H

G

G

Y

F

T

T

Y

Y

G

S

G

G

H

H

P

P

V

V

S

S

A

C

A

A

V

V

A

A

L

I

E

E

T

A

L

L

N

K

I

I

Y

Y

A

K

E

E

R

R

D

N

I

I

L

P

A

E

Q

Y

V

V

A

A

E

K

V

V

G

A

P

P

V

R

L

F

Q

Q

D

D

G

G

L

V

R

K

A

A

L

F

-

A

R

S

G

G

H

S

P

P

L

I

V

I

G

G

E

E

A

T

R

R

G

G

V

T

G

G

L

L

I

I

G

L

A

G

V

T

E

E

L

F

V

V

A

D

D

N

K

K

A

S

N

P

R

N

A

E

P

P

F

F

P

P

P

P

E

E

L

W

A

G

V

V

G

G

A

A

R

F

V

F

V

G

A

A

K

E

A

C

Q

Q

A

K

K

H

G

G

V

M

I

L

L

V

R

R

A

V

M

A

G

G

D

D

A

G

V

I

A

L

F

M

A

S

P

P

P

P

L

L

V

I

I

I

S

S

K

P

A

E

D

E

I

I

A

D

E

E

M

L

L

I

R

S

R

I

F

Y

G

G

L

K

A

A

L

L

C98 (= C61) mutation to Y: In her1-1; loss of E2-hexenal responses.
G103 (≠ W66) mutation to N: In her1-2; loss of E2-hexenal responses.
G271 (= G233) mutation to E: In pop2-6; Loss of activity and complete sterility.
G312 (= G274) mutation to D: In pop2-7; loss of activity.

D6R3B6 Vanillin aminotransferase; Putative aminotransferase; pAMT; EC 2.6.1.119 from Capsicum frutescens (Cayenne pepper) (Tabasco pepper) (see paper)
49% identity, 95% coverage: 18:452/459 of query aligns to 13:447/459 of D6R3B6

query
sites
D6R3B6

V

M

L

L

H

A

P

P

Y

F

T

T

N

-

L

A

K

G

R

W

H

Q

P

S

E

D

V

M

G

E

P

P

L

L

V

V

I

I

T

E

R

K

G

S

Q

K

G

G

V

S

R

Y

V

V

Y

Y

D

D

E

I

A

N

G

G

R

K

D

K

Y

Y

I

L

E

D

G

T

L

L

A

S

G

G

L

L

W

W

C

C

T

A

S

T

L

L

G

G

W

G

G

S

E

E

E

T

R

R

L

L

V

V

E

E

A

A

A

A

A

N

R

K

Q

Q

M

L

R

N

Q

T

L

L

P

P

F

F

Y

Y

H

H

L

S

F

F

S

W

H

N

K

R

T

T

H

T

D

K

V

P

G

S

V

L

E

D

L

L

C

A

A

K

R

E

L

L

L

L

A

N

M

M

A

F

P

T

V

A

-

N

P

K

M

M

S

A

K

K

V

V

F

F

L

F

A

T

G

N

S

S

G

G

S

S

E

E

A

A

N

N

D

D

T

T

A

Q

I

V

K

K

L

L

I

V

H

W

Y

Y

R

Y

A

N

N

N

A

A

L

L

G

G

T

R

P

P

D

Q

K

K

K

K

K

K

I

I

I

I

A

A

R

R

E

A

K

K

A

A

Y

Y

H

H

G

G

V

S

T

T

V

Y

A

I

T
x
S

A
|
A

S
x
G

L
|
L

T

S

G

G

L

L

V

P

N

P

N

M

Q

H

R

Q

S

K

F

F

D

D

L

L

P

P

I

P

P

P

G

F

V

V

L

L

R

H

A

T

A

E

C

C

P

P

H

H

H

Y

Y

W

R

A

F

Y

A

H

K

L

D

P

G

G

E

E

S

T

E

E

E

E

D

E

F

F

S

S

T

T

R

R

L

L

A

A

G

N

E

N

L

L

E

E

A

S

M

L

I

I

L

L

A

N

E

E

G

G

P

P

D

E

T

T

V

V

A

A

A

A

F

F

F

I

A

A

E

E

P

P

V

V

M

L

G

G

A

A

G

A

G

G

V

V

I

I

V

L

P

P

P

P

A

A

G

T

Y

Y

F

F

P

D

K

K

I

V

Q

Q

A

A

V

I

L

L

D

R

R

K

Y

H

D

D

V

I

L

L

L

F

V

I

V

A

D

D

E

E

V

V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

K

T

M

M

F

F

G

G

T

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T

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F

Y

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I

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D

M

L

M

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V

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A

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K

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A

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S

S

G

G

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Y

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M

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A

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M

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N

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H

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V

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A

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V

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L

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A

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I

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I

I

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V

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P

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G

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R

G

G

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T

G

G

L

L

I

A

G

L

A

S

V

T

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E

L

F

V

V

A

D

D

N

K

K

A

S

N

P

R

N

A

D

P

P

F

F

P

P

P

Y

E

E

L

W

A

A

V

V

G

G

A

T

R

Y

V

F

V

G

A

A

K

Q

A

C

Q

A

A

K

K

Y

G

G

V

M

I

L

L

V

R

S

A

S

M

T

G

G

D

D

A

H

V

V

A

N

F

M

A

A

P

P

P

P

L

F

V

T

I

L

S

S

K

L

A

E

D

E

I

L

A

D

E

E

M

L

L

I

R

R

R

I

F

Y

G

G

L

K

A

A

L

L

D

K

E

D

A

T

SAGL 155:158 (≠ TASL 160:163) mutation Missing: Low pungency phenotype, characterized by low levels of capsaicinoids, and accumulation of high levels of capsinoids, which are non-pungent capsaicinoid analogs.

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
47% identity, 97% coverage: 10:456/459 of query aligns to 9:460/460 of 5kr6B

query
sites
5kr6B

I

L

A

L

E

E

K

M

D

D

V

R

D

A

S

H

V

F

L

F

H

H

P

P

Y

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T

T

N

H

L

L

K

R

R

D

H

H

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A

-

S

-

G

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V

L

G

P

P

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I

I

I

T

T

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G

G

G

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K

G

G

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V

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Y

Q

D

D

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S

A

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G

G

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R

D

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Y

Y

I

I

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D

G

A

L

F

A

A

G

G

L

L

W

Y

C

C

T

V

S

N

L

V

G

G

W

Y

G

G

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R

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T

R

E

L

I

V

A

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D

A

A

A

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A

Y

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Q

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A

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Q

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L

L

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A

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Y

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H

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V

H

G

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H

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H

N

D

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P

G

I

V

I

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L

L

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A

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R

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L

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A

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M

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A

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M

M

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K

K

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Y

L

Y

A

G

G

M

S
|
S

G
|
G

S
|
S

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D

A

A

N
|
N

D

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T

T

A

Q

I

I

K

K

L

L

I

V

H

W

Y

Y

R

Y

A

N

N

N

A

V

L

L

G

G

T

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P

P

D

Q

K

K

K

K

K

K

I

I

I

I

A

S

R

R

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Q

K

R

A

G

Y
|
Y

H
|
H

G
|
G

V

S

T

G

V

I

A

M

T

T

A

G

S

S

L

L

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T

G

G

L

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N

A

N

F

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L

L

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A

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C

C

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P

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Y

Y

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-

A

A

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G

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L

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A

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D

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L

L

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A

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M

M

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I

L

L

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A

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G

G

P

P

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D

T

T

V

V

A

A

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F

F

F

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A

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|
E

P

P

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V

M

M

G

G

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G

G

G

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I

I

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V

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P

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Y

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W

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A

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I

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L

L

L

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|
D

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|
V

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x
V

C

C

G

G

F

F

G

G

R

R

T

T

G

G

K

S

M

M

F

F

G

G

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T

H

F

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G

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M

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M

L

M

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|
K

G

G

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Y

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M

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G

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H

G

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W

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|
T

Y

Y

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H

H

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P

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A

A

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A

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L

L

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N

L

L

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D

I

I

Y

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L

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K

K

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K

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F

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L

L

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K

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V

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G

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V

V

V

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A

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Q

L

A

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K

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G

G

V

M

I

I

L

A

R

R

A

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M

M

-

P

-

H

G

G

D

D

A

I

V

L

A

G

F

F

A

A

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P

P

P

L

L

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V

I

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S

T

K

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A

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D

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I

V

A

D

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M

I

L

V

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D

R

I

F

A

G

K

L

Q

A

A

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D

D

E

A

A

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H

A

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D

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L

L

binding pyridoxal-5'-phosphate: S121 (= S119), G122 (= G120), S123 (= S121), N126 (= N124), Y155 (= Y153), H156 (= H154), G157 (= G155), E228 (= E227), D261 (= D260), V263 (= V262), V264 (≠ I263), K290 (= K289), W326 (≠ Y325), T327 (= T326)

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
47% identity, 97% coverage: 6:451/459 of query aligns to 1:451/455 of 5kr5A

query
sites
5kr5A

S

S

N

L

R

E

T

Q

I

L

A

L

E

E

K

M

D

D

V

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D

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H

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H

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Y

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H

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G

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I

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G

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D

D

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A

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G

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Y

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I

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A

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A

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L

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Y

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V

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N

L

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G

W

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|
E

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L

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V

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x
D

A

A

A

I

A

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Q

M

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L

L

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Y

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H

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V

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H

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N

D

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V

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L

L

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A

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R

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L

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M

W

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A

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G

M

M

S

K

K

K

V

V

F

F

L

Y

A

G

G

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N
|
N

D

E

T

T

A

Q

I

I

K

K

L

L

I

V

H

W

Y

Y

R

Y

A

N

N

N

A

V

L

L

G

G

T

R

P

P

D

N

K

K

K

K

K

K

I

I

I

I

A

A

R

R

E

E

K

R

A

S

Y
|
Y

H
|
H

G
|
G

V

S

T

G

V

I

A

V

T

T

A

G

S

S

L

L

T

T

G

G

L

L

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N

S

N

F

Q

H

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F

F

D

D

L

L

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P

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V

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A

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C

C

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Y

Y

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G

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S

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Y

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E

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L

L

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A

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M

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I

L

A

A

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G

G

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A

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D

T

T

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I

A

A

A

A

F

F

F

I

A

A

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|
E

P

P

V

V

M

M

G

G

A

T

G

G

G

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I

I

V

V

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P

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A

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I

L

L

L

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|
D

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V
|
V

I

V

C

C

G

G

F

F

G

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L

G

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M

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G

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F

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M

L

M

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|
K

G

G

L

L

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T

S

S

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A

Y

Y

A

A

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P

I

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S

S

A

A

L

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M

I

V

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N

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V

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A

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K

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S

G

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I

H

G

G

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V

F

M

G

G

H

H

G

G

Y
x
W

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|
T

Y

Y

G

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G

G

H

H

P

P

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V

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C

A

A

A

A

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A

A

A

L

L

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A

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N

L

L

N

D

I

I

Y

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N

I

L

L

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A

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G

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L

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G

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A

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G

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V

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G

L

M

I

L

G

A

A

A

V

L

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E

L

F

V

M

A

A

D

D

K

K

A

G

N

A

R

R

A

T

P

P

F

F

P

D

P

P

E

A

L

L

A

K

V

V

G

S

A

Q

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K

V

V

V

A

A

A

K

A

A

A

Q

L

A

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K
x
D

G

G

V

L

I

I

L

V

R

R

A

A

M

L

-

P

-

H

G

G

D

D

A

I

V

L

A

G

F

F

A

A

P

P

P

P

L

L

V

V

I

T

S

T

K

R

A

A

D

E

I

V

A

D

E

E

M

I

L

V

R

A

R

I

F

A

G

K

L
x
E

A

A

L

F

D

D

E

E

binding calcium ion: E66 (= E69), D70 (≠ E73), D412 (≠ K414), E447 (≠ L447)
binding pyridoxal-5'-phosphate: S117 (= S119), G118 (= G120), S119 (= S121), N122 (= N124), Y151 (= Y153), H152 (= H154), G153 (= G155), E224 (= E227), D257 (= D260), V259 (= V262), K286 (= K289), W322 (≠ Y325), T323 (= T326)

5kquC Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
46% identity, 97% coverage: 12:456/459 of query aligns to 9:458/459 of 5kquC

query
sites
5kquC

E

E

K

Q

D

D

V

R

D

A

S

H

V

F

L

M

H

H

P

P

Y

S

T

T

N

H

L

A

K

H

R

D

H

H

P

A

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S

V

-

G

G

P

A

L

L

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P

-

G

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R

V

I

I

I

T

T

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G

G

A

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S

G

G

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Y

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D

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H

A

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G

G

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Y

L

I

I

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D

G

A

L

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A

A

G

G

L

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W

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C

C

T

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N

L

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G

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G

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R

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L

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V

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D

A

A

A

I

A

Y

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K

Q

Q

M

A

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K

Q

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L

L

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A

F

Y

Y

Y

H

H

L

T

F

Y

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V

H

G

K

H

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H

T

D

E

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A

G

I

V

I

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E

L

L

C

S

A

S

R

R

L

I

L

I

-

R

A

D

M

W

A

A

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P

V

A

P

G

M

M

S

K

K

K

V

V

F

Y

L

Y

A

G

G

L

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

T

T

A

Q

I

I

K

K

L

L

I

V

H

R

Y

Y

R

Y

A

N

N

N

A

V

L

L

G

G

T

R

P

P

D

Q

K

K

K

K

K

K

I

I

I

I

A

S

R

R

E

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K

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A

G

Y
|
Y

H
|
H

G
|
G

V

S

T

G

V

I

A

M

T

T

A

G

S

S

L

L

T

T

G

G

L

L

V

P

N

S

N

F

Q

H

R

Q

S

H

F

F

D

D

L

L

P

P

I

V

P

E

G

G

V

I

L

K

R

H

A

T

A

V

C

C

P

P

H

H

H

W

Y

Y

R

K

F

-

A

A

K

P

D

A

G

G

E

M

S

D

E

E

E

A

D

A

F

F

S

V

T

R

R

Y

L

C

A

A

G

D

E

E

L

L

E

E

A

K

M

L

I

I

L

L

A

A

E

E

G

G

P

P

D

D

T

T

V

V

A

A

A

A

F

F

F

I

A

G

E
|
E

P

P

V

V

M

M

G

G

A

T

G

G

G

G

V

I

I

I

V

V

P

P

P

P

A

K

G

G

Y

Y

F

W

P

E

K

A

I

I

Q

Q

A

A

V

V

L

L

D

N

R

K

Y

Y

D

D

V

V

L

L

L

L

V

I

V

A

D
|
D

E

E

V
|
V

I

V

C

C

G

A

F

F

G

G

R

R

T

L

G

G

K

S

M

K

F

M

G

G

T

S

E

Q

T

R

F

Y

G

G

I

M

R

R

P

P

D

D

M

L

M

I

T

T

L

T

A

A

K
|
K

G

G

L

L

S

T

S

S

G

A

Y

Y

A

A

P

P

I

L

S

S

A

A

L

V

M

I

V

V

N

G

E

E

R

K

V

V

Y

W

G

D

P

V

V

I

A

E

E

K

E

A

S

S

G

Q

R

K

I

E

G

G

V

A

F

M

G

G

H

H

G

G

Y
x
W

T
|
T

Y

Y

G

S

G

G

H

H

P

P

V

I

S

C

A

A

A

A

V

A

A

A

L

L

E

A

T

N

L

L

N

D

I

I

Y

L

A

E

E

R

R

E

D

N

I

L

L

T

A

A

Q

N

V

A

A

A

E

D

V

V

G

G

P

A

V

Y

L

L

Q

N

D

Q

G

R

L

L

R

R

-

E

A

T

L

F

R

E

G

G

H

H

P

P

L

L

V

V

G

G

E

E

A

V

R

R

G

G

V

D

G

G

L

M

I

L

G

A

A

A

V

L

E

E

L

F

V

M

A

A

D

D

K

R

A

E

N

A

R

R

A

T

P

P

F

F

P

D

P

P

E

A

L

L

A

K

V

V

G

G

A

P

R

K

V

V

V

S

A

A

K

A

A

C

Q

L

A

E

K

D

G

G

V

M

I

I

L

A

R

R

A

A

M

M

-

P

-

H

G

G

D

D

A

I

V

L

A

G

F

F

A

A

P

P

P

P

L

L

V

V

I

L

S

T

K

R

A

A

D

E

I

A

A

D

E

E

M

I

L

V

R

G

R

I

F

A

G

K

L

A

A

A

L

V

D

D

E

E

A

V

H

A

G

G

E

E

L

V

binding pyridoxal-5'-phosphate: S119 (= S119), G120 (= G120), S121 (= S121), Y153 (= Y153), H154 (= H154), G155 (= G155), E226 (= E227), D259 (= D260), V261 (= V262), K288 (= K289), W324 (≠ Y325), T325 (= T326)

5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
46% identity, 97% coverage: 12:456/459 of query aligns to 10:457/458 of 5kr3A

query
sites
5kr3A

E

E

K

M

D

D

V

R

D

A

S

H

V

F

L

M

H

H

P

P

Y

S

T

T

N

H

L

A

K

H

R

D

H

H

P

A

E

S

V

-

G

G

P

A

L

L

-

P

-

G

-

R

V

I

I

I

T

T

R

G

G

G

Q

K

G

G

V

I

R

R

V

I

Y

E

D

D

E

H

A

E

G

G

R

R

D

E

Y

Y

I

I

E

D

G

A

L

F

A

A

G

G

L

L

W

Y

C

C

T

V

S

N

L

I

G

G

W

Y

G

G

E

R

E

T

R

E

L

V

V

A

E

D

A

A

A

I

A

Y

R

E

Q

Q

M

A

R

K

Q

Q

L

L

P

A

F

Y

Y

Y

H

H

L

T

F

Y

S

V

H

G

K

H

T

S

H

T

D

D

V

A

G

I

V

I

E

E

L

L

C

S

A

S

R

R

L

I

L

I

-

R

A

D

M

W

A

A

P

P

V

A

P

G

M

M

S

K

K

K

V

V

F

Y

L

Y

A

G

G

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N
|
N

D

E

T

T

A

Q

I

I

K

K

L

L

I

V

H

W

Y

Y

R

Y

A

N

N

N

A

V

L

L

G

G

T

R

P

P

D

N

K

K

K

K

K

K

I

I

I

I

A

S

R

R

E

E

K

R

A

G

Y
|
Y

H
|
H

G
|
G

V

S

T

G

V

I

A

V

T

T

A

G

S

S

L

L

T

T

G

G

L

L

V

P

N

S

N

F

Q

H

R

Q

S

H

F

F

D

D

L

L

P

P

I

I

P

D

G

R

V

V

L

K

R

H

A

T

A

V

C

C

P

P

H

H

H

W

Y

Y

R

R

F

A

A

P

K

M

D

-

G

-

E

-

S

S

E

E

E

A

D

Q

F

F

S

V

T

A

R

Y

L

C

A

V

G

E

E

E

L

L

E

E

A

K

M

L

I

I

L

A

A

R

E

E

G

G

P

A

D

D

T

T

V

I

A

A

A

A

F

F

F

I

A

A

E
|
E

P

P

V

V

M

M

G

G

A

T

G

G

G

G

V

I

I

I

V

P

P

P

P

P

A

Q

G

G

Y

Y

F

W

P

E

K

A

I

I

Q

Q

A

A

V

V

L

L

D

R

R

K

Y

H

D

D

V

I

L

L

L

L

V

I

V

A

D
|
D

E

E

V
|
V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

K

S

M

K

F

M

G

G

T

S

E

Q

T

H

F

Y

G

G

I

I

R

K

P

P

D

D

M

L

M

I

T

T

L

V

A

A

K
|
K

G

G

L

L

S

T

S

S

G

A

Y

Y

A

A

P

P

I

L

S

S

A

G

L

V

M

I

V

V

N

G

E

E

R

K

V

V

Y

W

G

D

P

V

V

I

A

E

E

K

E

G

S

S

G

Q

R

E

I

H

G

G

V

P

F

M

G

G

H

H

G

G

Y
x
W

T
|
T

Y

Y

G

S

G

G

H

H

P

P

V

I

S

C

A

A

A

A

V

A

A

A

L

L

E

A

T

N

L

L

N

D

I

I

Y

L

A

E

E

R

R

E

D

N

I

L

L

T

A

G

Q

N

V

A

A

A

E

D

V

V

G

G

P

A

V

Y

L

L

Q

Q

D

Q

G

R

L

L

R

H

-

E

A

A

L

F

R

G

G

A

H

H

P

P

L

L

V

V

G

G

E

E

A

V

R

R

G

G

V

V

G

G

L

M

I

L

G

A

A

A

V

L

E

E

L

F

V

M

A

A

D

D

K

K

A

D

N

A

R

R

A

T

P

P

F

F

P

D

P

P

E

A

L

L

A

K

V

V

G

G

A

P

R

K

V

V

V

S

A

A

K

A

A

A

Q

L

A

E

K

D

G

G

V

M

I

I

L

A

R

R

A

A

M

M

-

P

-

H

G

G

D

D

A

I

V

L

A

G

F

F

A

A

P

P

P

P

L

L

V

V

I

T

S

T

K

R

A

A

D

E

I

V

A

D

E

E

M

I

L

V

R

G

R

I

F

A

G

K

L

Q

A

A

L

V

D

D

E

E

A

V

H

A

G

D

E

E

L

V

binding pyridoxal-5'-phosphate: S120 (= S119), G121 (= G120), S122 (= S121), N125 (= N124), Y154 (= Y153), H155 (= H154), G156 (= G155), E225 (= E227), D258 (= D260), V260 (= V262), K287 (= K289), W323 (≠ Y325), T324 (= T326)

3gjuA Crystal structure of a putative aminotransferase (mll7127) from mesorhizobium loti maff303099 at 1.55 a resolution
45% identity, 93% coverage: 14:438/459 of query aligns to 13:444/458 of 3gjuA

query
sites
3gjuA

D

D

V

R

D

D

S

H

V

F

L

F

H

H

P

P

Y
x
S

T

T

N

H

L

M

K

G

R

T

H

H

P

A

-

R

-