SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011384418.1 NCBI__GCF_000009985.1:WP_011384418.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7WTU0 Arsenate respiratory reductase molybdopterin-containing subunit ArrA; Arsenate respiratory reductase large subunit; ARR large subunit; EC 1.20.99.1 from Shewanella sp. (strain ANA-3) (see paper)
31% identity, 27% coverage: 8:253/911 of query aligns to 57:290/854 of Q7WTU0

query
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Q7WTU0

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C61 (= C12) binding
C64 (= C15) binding
C68 (= C23) binding
C96 (= C51) binding
R165 (≠ T122) binding
Y166 (≠ F123) binding
H189 (≠ F150) binding
S190 (≠ G151) binding
C193 (= C158) binding
K198 (vs. gap) binding
Y210 (≠ H170) binding

6czaA The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 bound to phosphate (see paper)
31% identity, 27% coverage: 8:253/911 of query aligns to 17:250/814 of 6czaA

query
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6czaA

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active site: R58 (≠ K53), C153 (= C158), A154 (≠ V159)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: C24 (= C15), T25 (≠ V16), R58 (≠ K53), H149 (≠ F150), V152 (≠ K157), C153 (= C158), G185 (= G189), A190 (≠ V194), S191 (= S195), N192 (≠ G196), D215 (≠ E218), P216 (= P219), R217 (≠ H220), P233 (= P236), D236 (= D239)
binding phosphate ion: Y126 (≠ F123), S150 (≠ G151), K158 (vs. gap)
binding iron/sulfur cluster: C21 (= C12), G23 (≠ N14), C24 (= C15), S26 (≠ A17), W27 (≠ M22), C28 (= C23), K30 (vs. gap), C56 (= C51), R58 (≠ K53), S196 (≠ G200)

Sites not aligning to the query:

active site: 334
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 333, 334, 334, 335, 441, 442, 443, 444, 447, 468, 469, 472, 484, 487, 521, 681, 682, 684, 684, 686, 688, 690, 690, 691, 692, 762, 769, 787, 803, 804

6cz9A The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 bound to arsenite (see paper)
31% identity, 27% coverage: 8:253/911 of query aligns to 17:250/814 of 6cz9A

query
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6cz9A

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active site: R58 (≠ K53), C153 (= C158), A154 (≠ V159)
binding arsenite: R125 (≠ T122), Y126 (≠ F123), H149 (≠ F150), C153 (= C158), Y170 (≠ H170)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: C24 (= C15), T25 (≠ V16), R58 (≠ K53), H149 (≠ F150), V152 (≠ K157), C153 (= C158), G185 (= G189), A190 (≠ V194), S191 (= S195), N192 (≠ G196), D215 (≠ E218), P216 (= P219), R217 (≠ H220), P233 (= P236), D236 (= D239)
binding iron/sulfur cluster: C21 (= C12), G23 (≠ N14), C24 (= C15), S26 (≠ A17), W27 (≠ M22), C28 (= C23), K30 (vs. gap), C56 (= C51), R58 (≠ K53), S196 (≠ G200)

Sites not aligning to the query:

active site: 334
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 333, 334, 334, 335, 441, 442, 443, 444, 447, 468, 469, 472, 484, 487, 521, 682, 684, 684, 685, 686, 688, 690, 690, 691, 692, 762, 769, 787, 803, 804

6cz8A The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 bound to arsenate (see paper)
31% identity, 27% coverage: 8:253/911 of query aligns to 17:250/814 of 6cz8A

query
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6cz8A

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active site: R58 (≠ K53), C153 (= C158), A154 (≠ V159)
binding arsenate: R125 (≠ T122), Y126 (≠ F123), H149 (≠ F150), C153 (= C158), Y170 (≠ H170)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: C24 (= C15), R58 (≠ K53), H149 (≠ F150), V152 (≠ K157), C153 (= C158), G185 (= G189), A190 (≠ V194), S191 (= S195), N192 (≠ G196), D215 (≠ E218), P216 (= P219), R217 (≠ H220), P233 (= P236), D236 (= D239)
binding oxygen atom: R125 (≠ T122), H149 (≠ F150)
binding iron/sulfur cluster: C21 (= C12), G23 (≠ N14), C24 (= C15), S26 (≠ A17), W27 (≠ M22), C28 (= C23), K30 (vs. gap), C56 (= C51), R58 (≠ K53), S196 (≠ G200)

Sites not aligning to the query:

active site: 334
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 333, 334, 334, 335, 441, 442, 443, 444, 447, 468, 469, 472, 484, 487, 521, 682, 684, 684, 686, 688, 690, 690, 691, 692, 762, 769, 787, 803, 804

6cz7A The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 (see paper)
31% identity, 27% coverage: 8:253/911 of query aligns to 17:250/814 of 6cz7A

query
sites
6cz7A

V

L

P

A

T

T

Y

T

C
|
C

Y

Q

N
x
G

C
|
C

V
x
T

A
x
S

G

-

P

-

D

-

L

-

M
x
W

C
|
C

-

A

-
x
K

-

Q

V

I

T

Y

V

V

K

M

D

D

G

G

V

R

A

A

V

L

A

K

V

V

T

R

P

G

N

N

H

-

A

P

G

N

M

S

G

G

I

V

H

H

P

G

A

M

D

S

G

S

R

-

P

-

C
|
C

V

P

K
x
R

A

Q

Y

H

G

L

L

S

V

L

Q

Q

K

Q

T

V

Y

Y

N

D

P

P

H

D

R

R

V

L

L

R

T

T

P

P

M

M

K

M

R

R

T

T

N

N

P

P

R

K

K

K

G

G

R

R

D

D

E

Q

D

D

P

P

G

K

F

F

V

V

P

P

I

I

S

S

W

W

D

D

E

K

A

A

L

L

D

D

L

M

V

L

A

A

G

D

K

K

L

I

R

I

S

A

V

L

R

R

E

-

M

-

G

-

L

V

L

A

N

N

E

E

-

P

Q

H

G

K

M

Y

P

A

R

L

L

L

A

R

A

G

T

R

F

Y

G

S

H

H

G

I

G

N

-

D

-

L

-

Y

-

K

T

K

P

M

A

T

N

N

Y

L

M

I

G

G

T

S

F

-

P

-

A

-

F

-

L

-

A

-

W

-

G

-

P

P

I

N

D

N

F

I

S

S

F
x
H

G

S

S

S

G

-

Q

-

G

-

V

-

K
x
V

C
|
C

V
x
A

H

E

S

A

E

H

-

K

-

M

-

G

-

P

-

Y

H

Y

L

L

Y

D

G

G

E

N

Y

W

W

G

H

Y

R

N

A

Q

F

Y

T

-

V

-

A

-

D

D

T

V

P

K

N

N

A

A

N

K

F

F

I

I

V

L

S

S

F

F

G
|
G

T

A

N

D

V

P

E

I

V
x
A

S
|
S

G
x
N

G

R

P

Q

C
x
V

G
x
S

V

F

R

Y

R

S

H

Q

A

T

-

W

D

G

A

D

R

S

I

L

R

D

G

H

I

A

K

K

R

V

V

V

Q

V

V

V

E
x
D

P
|
P

H
x
R

L

L

S

S

P

A

T

S

G

A

A

A

C

K

S

A

A

H

Q

K

W

W

V

I

P

P

I

I

R

E

P
|
P

K

G

T

Q

D
|
D

P

S

A

V

F

L

M

A

F

L

A

A

M

I

L

A

H

H

V

V

M

A

L

L

H

V

E

E

active site: R58 (≠ K53), C153 (= C158), A154 (≠ V159)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: C24 (= C15), T25 (≠ V16), R58 (≠ K53), H149 (≠ F150), V152 (≠ K157), C153 (= C158), G185 (= G189), A190 (≠ V194), S191 (= S195), N192 (≠ G196), D215 (≠ E218), P216 (= P219), R217 (≠ H220), P233 (= P236), D236 (= D239)
binding iron/sulfur cluster: C21 (= C12), G23 (≠ N14), C24 (= C15), S26 (≠ A17), W27 (≠ M22), C28 (= C23), K30 (vs. gap), C56 (= C51), R58 (≠ K53), V195 (≠ C199), S196 (≠ G200)

Sites not aligning to the query:

active site: 334
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 333, 334, 334, 335, 441, 442, 443, 444, 447, 468, 469, 472, 484, 487, 521, 681, 682, 682, 684, 684, 686, 688, 690, 690, 691, 692, 762, 769, 787, 803, 804

2vpwA Polysulfide reductase with bound menaquinone (see paper)
28% identity, 27% coverage: 4:253/911 of query aligns to 7:242/734 of 2vpwA

query
sites
2vpwA

E

E

T

V

K

K

S

S

V

V

P

Y

T

Q

Y

I

C
|
C

Y

E

N
x
G

C
|
C

V

F

A

-

G

-

P

-

D

-

L
x
W

M

R

C
|
C

V

G

T

I

V

V

K

A

D

H

G

A

V

V

A

G

V

N

A

R

V

V

T

Y

P

K

N

V

H

E

A

G

G

Y

M

E

G

A

I

N

H

P

P

K

A

S

D

R

G

G

R

R

P
x
L

C
|
C

V

P

K
x
R

A
x
G

Y

Q

G

G

L

A

V

P

Q

Q

K

T

T

T

Y

Y

N

D

P

P

H

D

R

R

V

L

L

K

T

R

P

P

M

L

K

I

R

R

T

V

N

-

P

-

R

-

K

E

G

G

R

S

D

Q

E

R

D

G

P

E

G

G

-

K

F

Y

V

R

P

V

I

A

S

T

W

W

D

E

E

E

A

A

L

L

D

D

L

H

V

I

A

A

G

K

K

K

L

M

R

L

S

E

V

I

R

R

E

E

M

-

G

-

L

-

N

-

E

-

Q

K

G

Y

M

G

P

P

R

E

L

A

A

I

A

A

T

F

F

F

G

G

H
|
H

G

G

G

T

T

G

P
x
D

A

Y

N

W

Y

F

M

V

G

D

T

F

F

L

P

P

A

A

F

-

L

-

A

-

A

A

W

W

G

G

P

S

I

P

D

N

F

-

S

-

F

-

G

A

S

A

G
x
K

Q

P

G

S

V

V

K

S

C
x
L

V
x
C

H
x
T

S

A

E

P

H

R

L

E

Y

V

G

A

E

S

Y

Q

W

W

H

V

R

F

A

G

F

R

T

P

V

I

A

G

D

H

T

E

P

P

-

I

-

D

-

W

-

E

N

N

A

A

N

R

F

Y

I

I

V

V

S

L

F

I

G
|
G

T
x
H

N
x
H

V

I

E

G

V

E

S
x
D

G
x
T

G
x
H

P
x
N

C

T

G

Q

V

L

R

Q

R

D

H

F

A

A

D

L

A

A

R

L

I

K

R

N

G

G

I

A

K

K

R

V

V

V

Q

V

V
|
V

E
x
D

P
|
P

H
x
R

L

F

S

S

P

T

T

A

G

A

A

A

C

K

S

A

A

H

Q

R

W

W

V

L

P

P

I

I

R

K

P

P

K

G

T

T

D
|
D

P

T

A

A

F

L

M

L

F

L

A

A

M

W

L

I

H

H

V

V

M

L

L

I

H

Y

E

E

active site: R52 (≠ K53), H116 (= H125), D120 (≠ P129), K138 (≠ G153), C144 (≠ V159), T145 (≠ H160)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: R52 (≠ K53), H116 (= H125), L143 (≠ C158), C144 (≠ V159), G178 (= G189), H179 (≠ T190), H180 (≠ N191), D184 (≠ S195), T185 (≠ G196), H186 (≠ G197), V206 (= V217), D207 (≠ E218), P208 (= P219), R209 (≠ H220), D228 (= D239)
binding iron/sulfur cluster: C15 (= C12), G17 (≠ N14), C18 (= C15), W20 (≠ L21), C22 (= C23), L49 (≠ P50), C50 (= C51), R52 (≠ K53), G53 (≠ A54), N187 (≠ P198)

Sites not aligning to the query:

active site: 303, 335, 340, 341, 351
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 302, 303, 304, 307, 308, 410, 411, 412, 415, 416, 436, 437, 441, 453, 459, 593, 594, 596, 596, 600, 601, 602, 602, 603, 604, 668, 687, 688, 706

A0A369NIV7 Dopamine dehydroxylase; EC 1.1.-.- from Eggerthella lenta (Eubacterium lentum) (see paper)
25% identity, 30% coverage: 4:274/911 of query aligns to 45:297/1017 of A0A369NIV7

query
sites
A0A369NIV7

E

D

T

T

K

V

S

A

V

V

P

K

T

T

Y

C

C

C

Y

R

N

G

C

C

V

G

A

K

G

M

P

E

D

C

L

G

M

V

C

K

V

V

T

I

V

V

K

Q

D

N

G

G

V

R

A

A

V

I

A

R

V

V

T

E

P

G

N

D

H

E

A

-

G

-

M

-

G

G

I

A

H

F

P

Q

A

S

D

M

G

G

R

N

P

C

C

C

V

T

K

K

A

S

Y

Q

G

S

L

S

V

I

Q

Q

K

A

T

A

Y

Y

N

H

P

P

H

D

R

R

V

L

L

H

T

Y

P

P

M

M

K

K

R

R

T

T

N

N

P

P

R

K

K

G

G

-

R

-

D

-

E

E

D

E

P

P

G

G

F

W

V

Q

P

R

I

I

S

S

W

W

D

D

E

E

A

A

L

M

D

Q

L

S

V

I

A

V

G

D

K

N

L

F

R

-

S

-

V

-

R

-

E

-

M

M

G

D

L

I

L

K

N

A

E

K

Q

H

G

G

M

G

P

E

R

A

L

I

A

A

A

C

T

Q

F

V

G

G

-

T

-

S

-

R

-

I

-

W

-

C

-

M

H

H

G

S

G

E

T

S

P

I

A

L

N

K

Y

N

M

M

G

L

T

E

F

T

P

P

A

N

F

N

L

V

A

E

A

A

W

W

-

Q

-

I

-

C

-

K

G

G

P

P

I

R

D

H

F

F

S

A

F

-

G

-

S

-

G

-

Q

-

G

-

V

-

K

-

C

-

V

T

H

T

S

M

E

V

H

S

L

Q

Y

F

G

A

E

M

Y

S

W

W

H

M

R

E

A

T

F

I

T

T

V

-

A

-

D

-

T

-

P

-

N

R

A

P

N

K

F

V

I

Y

V

V

S

Q

F

W

G

G

T

G

N

A

V

S

E

E

V

L

S

S

G

N

G

Y

P

D

C

D

G

S

V

C

R

R

R

T

H

T

A

V

D

D

A

V

R

A

I

S

R

R

G

A

I

D

K

V

R

H

V

I

Q

S

V

V

E

D

P

P

H

R

L

M

S

A

P

N

T

M

G

G

A

K

C

E

S

A

A

D

Q

Y

W

W

V

Q

P

H

I

L

R

R

P

P

K

G

T

T

D

D

P

G

A

A

F

L

M

A

F

L

A

A

M

W

L

T

H

N

V

V

M

I

L

I

H

E

E

-

A

-

P

-

R

K

S

K

A

L

L

Y

D

D

L

E

D

L

F

Y

L

V

R

K

D

K

R

W

T

T

A

N

S

A

P

P

Y

F

L

L

V

V

Sites not aligning to the query:

506 Crucial for dopamine dehydroxylation activity

2ivfA Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
22% identity, 62% coverage: 51:612/911 of query aligns to 63:622/912 of 2ivfA

query
sites
2ivfA

C
|
C

V

Q

K
|
K

A
x
G

Y

S

G

A

L

F

V

N

Q

N

K

N

T

L

Y

Y

N

G

P

D

H

E

R

R

V

V

L

K

T

Y

P

P

M

L

K

K

R

R

T

V

N

-

P

-

R

-

K

-

G

G

R

K

D

R

E

G

D

E

P

G

G

K

F

W

V

K

P

R

I

V

S

S

W

W

D

D

E

E

A

A

L

A

-

G

D

D

L

I

V

A

A

D

G

S

K

I

L

I

R

D

S

S

V

F

R

E

E

A

M

Q

G

G

L

-

N

-

E

S

Q

D

G

G

M

F

P

I

R

-

L

L

A

D

A

A

T
x
P

F
x
H

G

V

H
|
H

G
x
A

G

G

T

S

P

I

A

A

N

W

Y

G

M

A

G

G

T

F

F

R

P

M

A

T

F

Y

L

L

A

M

A

D

W

G

G

V

P

S

I

P

D

D

F

I

S

N

F

V

G
x
D

S
x
I

G

G

Q
x
D

G

T

V

Y

K

-

C

-

V

M

H

G

S

A

E

F

H

H

L

T

Y

F

G

G

E

K

Y

M

W

-

H

H

R

M

A

G

F

Y

T

S

V

-

A

A

A

D

D

N

T

L

P

L

N

D

A

A

N

E

F

L

I

I

V

F

S

M

F

T

G
x
C

T
x
S

N
|
N

V

W

E

S

V
x
Y

S
x
T

G

Y

G

-

P

P

C

S

G

S

V

Y

R

H

R

F

H

L

A

S

D

E

A

A

R

R

I

Y

R

K

G

G

I

A

K

E

R

V

V

V

Q

V

V
x
I

E

A

P
|
P

H
x
D

L

F

S
x
N

P

P

T

T

G

T

A

P

C

A

S

A

A

D

Q

L

W

H

V

V

P

P

I

V

R

R

P

V

K
x
G

T

S

D
|
D

P

A

A

A

F

F

M

W

F

L

A

G

M

L

L

S

H

Q

V

V

M

M

L

I

H

D

E

E

A

-

P

-

R

-

S

K

A

L

L

F

D

D

L

R

D

Q

F

F

L

V

R

C

D

E

R

Q

T

T

A

D

S

L

P

P

Y

L

L

L

V

V

D

R

-

M

D

D

E

T

G

G

W

K

Y

F

L

L

R

S

-

A

-

E

-

D

-

V

D

D

E

G

A

G

T

E

G

A

K

K

P

Q

L

F

M

Y

W

F

D

F

G

D

I

E

T

K

G

A

Q

G

P

S

V

V

P

R

H

K

D

A

H

S

P

R

G

G

A

T

V

L

-

K

-

L

-

D

-

F

-

M

P

P

A

A

L

L

E

E

G

G

S

T

F

F

T

S

V

-

P

-

A

-

V

-

A

A

K

R

L

L

P

K

D

N

G

G

E

K

M

T

V

I

R

Q

R

-

E

-

N

-

A

-

T

-

A

V

R

R

T

T

A

V

F

F

T

E

L

G

L

L

V

R

D

E

G

H

M

L

R

K

G

D

Y

Y

T

T

P

P

D

E

W

K

A

A

E

S

T

A

I

K

C

C

D

G

V

V

P

P

A

V

K

S

V

L

V

I

R

R

Q

E

V

L

A

G

L

R

E

K

Y

V

L

A

D

K

N

K

A

R

C

T

V

C

G

S

Q

Y

T

I

T
x
G

E
x
F

V
x
S

D

S

G

A

K
|
K

V

S

-

Y

-

H

-

G

-

D

L

L

P

M

H

E

R

R

P

S

V

L

A

F

V

L

T

A

L

M

G

A

K

L

T

S

V

G

N

N

-

W

-

G

-

K

N

P

G

G

W

T

G

G

A

A

Y

F

E

A

C

W

C

A

W

Y

A

S

R

D

T

D

V

N

L

M

A

V

T

Y

L

L

V

-

G

G

A

V

L
x
M

E

S

V

K

P

P

-

T

-

A

G

Q

G

G

T

G

L

M

G

D

T

E

T

L

V

H

R

Q

L

M

N

A

R

E

P

G

Y

F

E

N

N

K

R

R

L

T

K

L

S

E

V

A

K

D

P

P

-

T

-

S

-

T

G

D

E

E

D

M

G

G

F

N

M

I

N

E

A

F

Q

M

M

K

N

V

S

V

T

T

K

S

K

A

G

V

G

G

W

L

A

V

E

P

K

P

P

A

K

M

G

W

R

L

N

Y

A

Y

H

H

T

V

T

G

L

Y

I

D

P

Q

I

L

V

W

G

N

D

N

S

K

S

A

W

W

A

T

Q

D

-

P

A

A

L

L

G

K

P

K

T

S

H

F

L

G

A

A

W

Y

M

L

F

D

L

E

K

A

D

K

S

E

P

K

A

G

E

W

W

W

-

T

-

N

-

D

-

H

-

I

-

R

-

P

A

A

P

P

P

D

E

K

P

T

P

P

D

Q

V

V

W

Y

I

M

A

L

Y

L

R
x
S

T
x
Q

N
|
N

P

P

A

M

I
x
R

S
x
R

F

K

W
x
R

D

S

T

G

A

A

Q

K

L

M

S

F

G

P

N

D

M

V

A

L

-

F

-

P

K

K

M

L

P

K

F

M

V

I

V

F

C

A

F
x
L

A
x
E

Y
x
T

T
x
R

M

M

D

S

E

S

T

S

N

A

H

M

M

Y

A

A

D

D

I

I

L

V

L

L

P

P

E
x
C

A

A

T
x
W

D
x
Y

L

Y

E

E

S
x
K

T

H

Q

E

L

M

I

T

R

T

-

P

A

C

G

S

G

G

S

N

K

P

F

F

V

-

E

-

Q

-

H

-

W

-

Q

-

H

-

Q

-

G

-

F

F

V

T

L

F

R

V

Q

D

P

R

A

S

V

V

A

A

P

P

Q

P

G

G

E

E

S

C

R

R

D

E

active site: K65 (= K53), P127 (≠ T122), D156 (≠ G151), I157 (≠ S152), M430 (≠ L427)
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: H128 (≠ F123), H130 (= H125), A131 (≠ G126), I157 (≠ S152), D159 (≠ Q154), K386 (= K389), S543 (≠ R527), Q544 (≠ T528), N545 (= N529), R548 (≠ I532), R549 (≠ S533), R551 (≠ W535), L571 (≠ F553), E572 (≠ A554), T573 (≠ Y555), R574 (≠ T556), C588 (≠ E570), W590 (≠ T572), Y591 (≠ D573), K594 (≠ S576)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D159 (≠ Q154), C190 (≠ G189), S191 (≠ T190), N192 (= N191), Y195 (≠ V194), T196 (≠ S195), I217 (≠ V217), P219 (= P219), D220 (≠ H220), N222 (≠ S222), G237 (≠ K237), D239 (= D239), G381 (≠ T384), F382 (≠ E385), S383 (≠ V386)
binding iron/sulfur cluster: C63 (= C51), G66 (≠ A54)

Sites not aligning to the query:

7bkbD Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
27% identity, 29% coverage: 6:268/911 of query aligns to 5:246/549 of 7bkbD

query
sites
7bkbD

K

K

S

Y

V

V

P

A

T

T

Y

T

C
|
C

Y

P

N
x
Y

C
|
C

V

G

A

V

G

G

P
x
C

D

T

L

L

M

N

C

L

V

V

T

-

V

V

K

S

D

N

G

G

V

K

A

V

V

V

A

G

V

V

T

E

P

P

N

N

H

Q

A

R

G

S

M

-

G

-

I

-

H

P

P

I

A

N

D

E

G

G

R

K

P

L

C
|
C

V

P

K
|
K

A
x
G

Y

V

G

T

L

C

V

W

Q

E

K

H

T

I

Y

H

N

S

P

P

H

D

R

R

V

L

L

T

T

T

P

P

M

L

K

I

R

K

T

-

N

-

P

-

R

-

K

-

G

-

R

-

D

-

E

K

D

D

P

G

G

K

F

F

V

I

P

E

I

A

S

S

W

W

D

D

E

E

A

A

L

L

D

D

L

L

V

V

A

A

G

K

K

N

L

L

R

K

S

V

V

I

R

Y

E

D

-

K

-

H

-

G

-

P

-

K

-

G

M

L

G

G

L

F

-

Q

-

T

-

S

-

C

-

R

-

T

L

V

N

N

E

E

Q

D

G

C

M

Y

P

-

R

-

L

I

A

F

A

Q

T

K

F

F

G

A

H

R

G

V

G

G

T

F

P

K

A

T

N

N

Y

N

M

V

G

D

T

N

F

C

P

A

A

R

F

I

L

-

A

-

A

C

W

H

G

G

P

P

-

S

-

V

-

A

-

G

I

L

D

S

F

L

S

S

F

F

G

G

S

S

G

G

Q

A

G

A

V

T

K

N

C

G

V

F

H

E

S

-

E

-

H

-

L

-

Y

-

G

-

E

-

Y

-

W

-

H

-

R

-

A

-

F

-

T

-

V

-

A

-

D

D

T

A

P

L

N

N

A

A

N

D

F

L

I

I

V

L

S

I

F

W

G

G

T

S

N

N

V

A

E

-

V

V

S

E

G

A

G

H

P

P

C
x
L

G

A

V

G

R

R

H

I

A

A

D

Q

A

A

R

K

I

K

R

K

G

G

I

I

K

Q

R

I

V

I

Q

A

V

V

E

D

P

P

H

R

L

Y

S

T

P

M

T

T

G

A

A

R

C

L

S

A

A

D

Q

T

W

Y

V

V

P

R

I

F

R

N

P

P

K

S

T

T

D

H

P

I

A

A

F

L

M

A

F

N

A

S

M

M

L

M

H

Y

V

W

M

I

L

I

H

K

E

E

A

G

P

L

R

E

S

-

A

-

L

-

D

D

L

K

D

K

F

F

L

I

R

Q

D

D

R

R

T

V

binding iron/sulfur cluster: C11 (= C12), Y13 (≠ N14), C14 (= C15), C18 (≠ P19), C46 (= C51), K48 (= K53), G49 (≠ A54), L180 (≠ C199)

5chcA Crystal structure of the perchlorate reductase pcrab - substrate analog seo3 bound - from azospira suillum ps (see paper)
24% identity, 40% coverage: 12:374/911 of query aligns to 36:406/895 of 5chcA

query
sites
5chcA

C
|
C

Y

P

N

H

C

F

V

V

A

Y

G

S

P

K

D

D

L

G

M

V

C

V

V

M

T

R

V

E

K

E

D

Q

G

S

V

K

A

D

V

I

A

A

V

P

T

M

P

P

N

N

H

-

A

-

G

-

M

-

G

-

I

I

H

P

P

E

A

Y

D

N

G

P

R

R

P
x
G

C
|
C

V

N

K
|
K

A
x
G

Y

E

G

C

L

G

V

H

Q

D

K

Y

T

M

Y

Y

N

G

P

P

H

H

R

R

V

I

L

K

T

Y

P

P

M

L

K

I

R

R

T

V

N

G

P

E

R

R

-

G

K

E

G

G

R

K

D

-

E

-

D

-

P

-

G

-

F

W

V

R

P

R

I

A

S

T

W

W

D

E

E

E

A

A

L

L

D

D

L

M

V

I

A

A

G

D

K

K

-

C

-

V

-

D

-

T

L

I

R

K

S

N

V

H

R

A

E

P

M

D

G

C

L

I

L

S

N

V

E

Y

Q

S

G
x
P

M

V

P
|
P

R

A

L

V

A

S

-

P

A

V

T

S

F

F

G

S

H

A

G

G

G

H

T

R

P

F

A

A

N

H

Y

Y

M

I

G

G

T

A

F

H

P

A

A

H

F

T

L
x
F

A

Y

A

D

W
|
W

G
x
Y

P

G

I

-

D
|
D

F

H

S
x
P

F

T

G

G

S

Q

G

T

Q

Q

-

T

-

C

G

G

V

V

K

Q

C

G

V

D

H

T

S

C

E

E

H

-

L

-

Y

-

G

-

E

-

Y

-

W

-

H

-

R

-

A

-

F

-

T

-

V

-

A

T

A

A

D

D

T

W

P

F

N

N

A

S

N

K

F

Y

I

I

V

I

S

L

F

W

G
|
G

T

S

N

N

V

P

E

T

V
x
Q

S
x
T

G

R

G

I

P

P

C

-

G

D

V

A

R

H

R

F

H

L

A

S

D

E

A

A

R

Q

I

L

R

N

G

G

I

A

K

K

R

I

V

V

Q

S

V
x
I

E

S

P
|
P

H
x
D

L

Y

S
x
N

P

S

T

S

G

T

A

I

C

K

S

V

A

D

Q

K

W

W

V

I

P

H

I

P

R

Q

P

P

K

G

T

T

D
|
D

P

G

A

A

F

L

M

A

F

M

A

A

M

M

L

A

H

H

V

V

M

I

L

I

H

K

E

E

A

-

P

-

R

-

S

K

A

L

L

Y

D

D

L

A

D

H

F

S

L

L

R

K

D

E

R

Q

T

T

A

D

S

L

P

S

Y

Y

L

L

V

V

-

R

D

S

D

D

E

T

G

K

W

R

Y

F

L

L

R

R

D

E

E

A

-

D

-

V

-

A

-

G

A

S

T

K

G

D

K

K

P

F

L

Y

M

F

W

W

D

N

G

A

I

K

T

T

G

G

Q

K

P

P

V

V

-

I

P

P

H

K

D

G

H

S

P

W

G

G

A

D

V

Q

P

P

A

E

L

K

E

K

G

G

S

S

-

P

-

V

-

G

-

F

-

L

-

G

-

R

-

N

-

T

F

F

T

A

V

F

P

P

A

K

A

G

V

Y

A

I

K

D

L

L

P

G

D

D

-

L

-

D

-

P

-

A

-

L

-

E

-

G

-

K

-

F

-

N

G

M

E

Q

M

L

V

L

R

D

R

G

E

K

N

T

A

V

T

E

A

V

R

R

T

P

A

V

F

F

T

E

L

I

L

L

V

K

D

S

G

R

-

L

M

M

R

A

G

D

Y

N

T

T

P

P

D

E

W

K

A

A

E

A

T

K

I

I

C

T

D

G

V

V

P

T

A

A

K

K

V

A

V

I

R

T

Q

E

V

L

A

A

L

R

E

E

Y

F

active site: K72 (= K53), P134 (≠ G115), P136 (= P117), W163 (= W143), Y164 (≠ G144), D166 (= D147), P168 (≠ S149)
binding biselenite ion: F160 (≠ L140), Y164 (≠ G144), D166 (= D147)
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: Y164 (≠ G144), D166 (= D147)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D166 (= D147), G197 (= G189), Q202 (≠ V194), T203 (≠ S195), I224 (≠ V217), P226 (= P219), D227 (≠ H220), N229 (≠ S222), D246 (= D239)
binding molybdenum atom: D166 (= D147)
binding iron/sulfur cluster: C36 (= C12), G69 (≠ P50), C70 (= C51), G73 (≠ A54)

Sites not aligning to the query:

active site: 453, 456
binding biselenite ion: 455
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: 422, 533, 534, 535, 539, 560, 561, 563, 565, 580, 611, 758, 760, 766, 767, 768, 831, 845, 877
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 417, 418, 419, 423, 454, 455, 757, 759, 760, 761, 764, 765, 766, 826, 878
binding molybdenum atom: 455
binding iron/sulfur cluster: 28, 32, 34

5ch7A Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
24% identity, 40% coverage: 12:374/911 of query aligns to 35:405/894 of 5ch7A

query
sites
5ch7A

C
|
C

Y

P

N

H

C

F

V

V

A

Y

G

S

P

K

D

D

L

G

M

V

C

V

V

M

T

R

V

E

K

E

D

Q

G

S

V

K

A

D

V

I

A

A

V

P

T

M

P

P

N

N

H

-

A

-

G

-

M

-

G

-

I

I

H

P

P

E

A

Y

D

N

G

P

R

R

P
x
G

C
|
C

V

N

K
|
K

A
x
G

Y

E

G

C

L

G

V
x
H

Q
x
D

K

Y

T

M

Y

Y

N

G

P

P

H

H

R

R

V

I

L

K

T

Y

P

P

M

L

K

I

R

R

T

V

N

G

P

E

R

R

-

G

K

E

G

G

R

K

D

-

E

-

D

-

P

-

G

-

F

W

V

R

P

R

I

A

S

T

W

W

D

E

E

E

A

A

L

L

D

D

L

M

V

I

A

A

G

D

K

K

-

C

-

V

-

D

-

T

L

I

R

K

S

N

V

H

R

A

E

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R

A

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-

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F

F

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A

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D

-

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-

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-

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-

L

-

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-

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-

K

-

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-

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E

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F

active site: K71 (= K53), P133 (≠ G115), P135 (= P117), W162 (= W143), Y163 (≠ G144), D165 (= D147), P167 (≠ S149)
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: Y163 (≠ G144), D165 (= D147)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D165 (= D147), G196 (= G189), N198 (= N191), Q201 (≠ V194), T202 (≠ S195), I223 (≠ V217), P225 (= P219), D226 (≠ H220), N228 (≠ S222), D245 (= D239)
binding molybdenum atom: D165 (= D147)
binding iron/sulfur cluster: C35 (= C12), G68 (≠ P50), C69 (= C51), G72 (≠ A54)
binding zinc ion: H76 (≠ V58), D77 (≠ Q59)

Sites not aligning to the query:

active site: 452, 455
binding phosphoric acid 4-(2-amino-4-oxo-3,4,5,6,-tetrahydro-pteridin-6-yl)-2-hydroxy-3,4-dimercapto-but-3-en-yl ester guanylate ester: 421, 532, 533, 534, 538, 559, 560, 561, 562, 564, 576, 579, 582, 610, 757, 759, 765, 766, 767, 830, 844, 876
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 416, 417, 418, 422, 453, 454, 756, 758, 759, 760, 763, 764, 765, 825, 877
binding molybdenum atom: 454
binding iron/sulfur cluster: 27, 31, 33
binding sulfite ion: 799, 891, 893

P18775 Dimethyl sulfoxide reductase DmsA; DMSO reductase; DMSOR; Me2SO reductase; EC 1.8.5.3 from Escherichia coli (strain K12) (see 4 papers)
25% identity, 24% coverage: 42:264/911 of query aligns to 97:315/814 of P18775

query
sites
P18775

G

G

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F

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A

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C104 (= C51) mutation to S: No alteration of the growth, expression, or catalytic activities.
R106 (≠ K53) mutation to S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry.

Sites not aligning to the query:

1:45 signal peptide, Tat-type signal
17 R→S: Not targeted to the membrane, does not support anaerobic growth.
57 K→D: No alteration of the growth, expression, or catalytic activities.
67 C→S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry.
71 C→S: Cannot support growth.

O30078 Tetrathionate reductase subunit A; Tetrathionate reductase molybdenum subunit; EC 1.8.-.- from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
21% identity, 82% coverage: 148:897/911 of query aligns to 283:1048/1134 of O30078

query
sites
O30078

F

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P

H

W

E

T

A

D

G

T

I

V

Q

Y

W

F

W

W

D

S

E

E

Q

K

L

L

-

A

-

K

-

A

-

R

-

N

-

S

-

V

-

T

-

G

E

E

E

K

Y

F

Q

Y

G

G

I

G

P

P

H

K

W

Y

-

L

-

P

-

A

-

T

-

Y

-

A

-

P

H

L

D

G

V

T

P

E

G

R

R

R

W

F

V

Y

K

G

A

T

V

P

E

L

R

R

A

E

G

I

A

Y

K

P

A

E

E

S

D

Q

F

Y

P

P

F

F

W

L

G

V

I

V

T

P

K

G

T

S

M

P

P

L

Y

F

T

T

T

K

G

H

N

R

N

S

A

M

G

F

I

Y

P

Y

L

W

M

L

N

K

E

Q

V

V

A

M

G

-

N

-

L

-

R

-

G

P

H

E

G

N

S

F

V

A

I

V

I

I

N

N

A

P

S

E

S

D

A

A

A

E

K

K

L

L

D

G

I

I

K

E

D

S

G

G

D

D

W

V

V

I

E

K

V

I

S

V

S

T

V

P

V

T

G

G

H

E

T

L

R

E

G

V

R

V

A

A

L

V

V

E

Q

P

G

T

C

V

R

V

P

K

D

G

T

T

V

I

V

A

I

I

P

P

G

V

Q

G

F

M

Q

G

H

R

W

W

K

A

T

D

P

S

Y

A

A

V

K

K

D

K

L

P

N

A

F

Y

P

F

S

R

L

L

N

N

-

D

-

G

T

S

I

V

T

A

Q

A

M

L

S

V

L

S

E

E

L

L

T

P

D

D

A

G

T

A

G

S

S

L

G

P

A

S

D

D

V

A

V

V

Sites not aligning to the query:

6 R→Q: Does not affect TtrD binding.
15 V→Q: Strong decrease in TtrD binding.
17 S→Q: Does not affect TtrD binding.
20 V→Q: Does not affect TtrD binding.
22 L→Q: Does not affect TtrD binding.
24 G→Q: Does not affect TtrD binding.

7l5sA Crystal structure of haemophilus influenzae mtsz at ph 5.5 (see paper)
28% identity, 29% coverage: 24:289/911 of query aligns to 16:284/771 of 7l5sA

query
sites
7l5sA

V

V

T

V

V

V

K

Q

D

D

G

G

V

K

A

V

V

V

A

-

V

-

T

-

P

-

N

-

H

K

A

S

G

G

M

P

G

A

I

I

H

E

P

P

A

A

D

-

G

-

R

V

P

P

C

N

V

E

K
x
L

A

Q

Y

T

G

V

L

V

V

A

Q

D

K

Q

T

L

Y

Y

N

S

P

E

H

A

R

R

V

V

L

K

T

C

P

P

M

M

K

V

R

R

-

K

-

G

-

F

-

L

T

A

N

N

P

P

R

G

K

K

-

S

-

D

-

T

-

M

-

R

G

G

R

R

D

D

E

E

D

-

P

-

G

-

F

W

V

V

P

R

I

V

S

S

W

W

D

D

E

E

A

A

L

L

D

D

L

L

V

V

A

H

G

N

K

Q

L

L

R

K

S

R

V

V

R

R

E

-

M

-

G

-

L

-

N

D

E

E

Q

H

G

G

M

S

P

T

R

G

L

I

A

F

A

A

T

G

-

S

-
x
Y

-
x
G

-
x
W

F
|
F

G
x
S

H

C

G

G

G

S

T

L

P

H

A

A

N
x
S

-

R

-

T

-

L

-

Q

-

R

Y

Y

M

M

G

N

T

A

F

T

P

G

A

G

F

F

L

V

A

G

A

H

W

K

G

G

P

-

I

-

D
|
D

F
x
Y

S
|
S

F

T

G

G

S

A

G

A

Q

Q

G

-

V

-

K

-

C

-

V
|
V

H
x
I

S

M

E

P

H

H

L

V

Y

L

G

G

-

T

-

I

E

E

Y

V

W

Y

H

E

R

Q

A

Q

F

T

T

S

V

W

A

E

A

S

D

I

T

L

P

E

N

S

A

S

N

D

F

I

I

I

V

V

S

L

F

W

G
x
S

T

A

N

N

-

P

-

L

-
x
T

-

M

-

R

-
x
I

V

A

E

W

V

M

S

S

G

T

G

D

P

Q

C

K

G

G

V

I

R

E

R

Y

H

F

A

K

D

K

A

F

R

Q

I

A

R

S

G

G

I

K

K

R

R

I

V

I

Q

C

V
x
I

E
x
D

P
|
P

H
x
Q

L

K

S

S

P

E

T

T

-

C

G

Q

A

M

C

L

S

N

A

A

Q

E

W

W

V

I

P

P

I

V

R

N

P

T

K

A

T

T

D
|
D

P

V

A

P

F

L

M

M

F

L

A

G

M

I

L

A

H

H

V

T

M

L

L

V

H

E

E

Q

A

G

P

K

R

H

S

-

A

-

L

-

D

D

L

K

D

D

F

F

L

L

R

K

D

K

R

Y

T

T

A

-

S

S

P

G

Y

Y

L

A

V

K

D

F

D

E

E

E

G

-

W

-

Y

Y

L

L

R

L

D

G

E

K

A

T

T

D

G

G

K

Q

P

P

active site: L38 (≠ K53), W113 (vs. gap), D142 (= D147), V150 (= V159), I151 (≠ H160)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: Y111 (vs. gap), G112 (vs. gap), W113 (vs. gap), W113 (vs. gap), F114 (= F123), S115 (≠ G124), S122 (≠ N131), Y143 (≠ F148), S144 (= S149), S144 (= S149), S182 (≠ G189), T187 (vs. gap), T188 (vs. gap), I191 (vs. gap), I217 (≠ V217), D218 (≠ E218), P219 (= P219), Q220 (≠ H220), D240 (= D239)

Sites not aligning to the query:

active site: 319, 355, 356, 357
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 319, 320, 321, 323, 323, 324, 356, 422, 423, 424, 428, 430, 448, 449, 450, 465, 471, 501, 633, 634, 635, 635, 636, 639, 640, 641, 641, 642, 643, 707, 728, 745

2v45A A new catalytic mechanism of periplasmic nitrate reductase from desulfovibrio desulfuricans atcc 27774 from crystallographic and epr data and based on detailed analysis of the sixth ligand (see paper)
29% identity, 27% coverage: 6:253/911 of query aligns to 7:239/723 of 2v45A

query
sites
2v45A

K

K

S

W

V

V

P

K

T

G

Y

V

C
|
C

Y
x
R

N

Y

C
|
C

V

G

A
x
T

G

G

P
x
C

D

G

L

V

M

L

C

-

V

V

T

G

V

V

K

K

D

D

G

G

V

K

A

A

V

V

A

A

V

I

-

Q

-

G

T

N

P

P

N

N

H

N

A

H

G

N

M

A

G

G

I

L

H
x
L

P

-

A

-

D

-

G

-

R

-

P

-

C
|
C

V

L

K
|
K

A

G

Y

S

G

L

L

L

V

I

Q

P

K

V

T

L

Y

N

N

S

P

K

H

E

R

R

V

V

L

T

T

Q

P

P

M

L

K

V

R

R

T

R

N

H

P

-

R

K

K

G

G

G

R

K

D

L

E

E

D

-

P

-

G

-

F

-

V

-

P

P

I

V

S

S

W

W

D

D

E

E

A

A

L

L

D

D

L

L

V

M

A

A

G

S

K

R

L

F

R

R

S

S

V

S

R

I

E

D

M

M

-

Y

-

G

-

P

-

N

-

S

-

V

-

A

-

W

-

Y

-

G

-
x
S

-

G

G
x
Q

L

C

L

L

N

T

E

E

Q

E

G

S

M

Y

P

-

R

-

L

V

A

A

A

N

T

K

F

I

G

F

H

K

G

G

T

F

P

G

A

T

N

N

Y

N

M

V

G

D

T

G

F

N

P

P

A

R

F

L

L
x
C

-
x
M

-

A

A

S

A

A

W

V

G

G

P

G

I

Y

D

V

F

T

S

S

F

F

G

G

S

K

G

D

Q

E

G

P

V

M

K

-

C

-

V

-

H

-

S

-

E

-

H

-

L

-

Y

-

G

G

E

T

Y

Y

W

-

H

-

R

-

A

-

F

-

T

-

V

-

A

-

A

A

D

D

T

I

P

D

N

Q

A

A

N

T

F

C

I

F

V

F

S

I

F

I

G
|
G

T

S

N
|
N

V

T

E

S

V

-

S
x
E

G
x
A

G

H

P

P

C
x
V

G

L

V

F

R

R

H

I

A

A

D

R

A

R

R

K

I

Q

R

V

-

E

-

P

G

G

I

V

K

K

R

I

V

I

Q

V

V

A

E
x
D

P
|
P

H
x
R

L

R

S

T

P

N

T

T

G

S

A

R

C

I

S

A

A

D

Q

M

W

H

V

V

P

A

I

F

R

R

P
|
P

K

G

T

T

D
|
D

P

L

A

A

F

F

M

M

F

H

A

S

M

M

L

A

H

W

V

V

M

I

L

I

H

N

E

E

active site: K49 (= K53), S109 (vs. gap), C140 (≠ L140), M141 (vs. gap), A180 (≠ G196)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: R14 (≠ Y13), K49 (= K53), Q111 (≠ G109), C140 (≠ L140), G174 (= G189), N176 (= N191), E179 (≠ S195), A180 (≠ G196), D204 (≠ E218), P205 (= P219), R206 (≠ H220), P222 (= P236), D225 (= D239)
binding iron/sulfur cluster: C13 (= C12), C16 (= C15), T18 (≠ A17), C20 (≠ P19), L46 (≠ H44), C47 (= C51), V183 (≠ C199)

Sites not aligning to the query:

active site: 308, 344, 345, 346
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 307, 308, 309, 312, 313, 345, 346, 346, 416, 417, 418, 422, 443, 444, 445, 446, 461, 463, 615, 616, 617, 617, 618, 619, 621, 622, 623, 623, 624, 626, 689, 697, 713, 714

P81186 Periplasmic nitrate reductase; EC 1.9.6.1 from Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB) (see 3 papers)
29% identity, 27% coverage: 6:253/911 of query aligns to 39:271/755 of P81186

query
sites
P81186

K

K

S

W

V

V

P

K

T

G

Y

V

C
|
C

Y

R

N

Y

C
|
C

V

G

A

T

G

G

P
x
C

D

G

L

V

M

L

C

-

V

V

T

G

V

V

K

K

D

D

G

G

V

K

A

A

V

V

A

A

V

I

T

Q

-

G

-

D

P

P

N

N

H

N

A

H

G

N

M

A

G

G

I

L

H

L

P

-

A

-

D

-

G

-

R

-

P

-

C
|
C

V

L

K
|
K

A

G

Y

S

G

L

L

L

V

I

Q

P

K

V

T

L

Y

N

N

S

P

K

H

E

R

R

V

V

L

T

T

Q

P

P

M

L

K

V

R

R

T

R

N

H

P

-

R

K

K

G

G

G

R

K

D

L

E

E

D

-

P

-

G

-

F

-

V

-

P

P

I

V

S

S

W

W

D

D

E

E

A

A

L

L

D

D

L

L

V

M

A

A

G

S

K

R

L

F

R

R

S

S

V

S

R

I

E

D

M

M

-

Y

-

G

-

P

-

N

-

S

-

V

-

A

-

W

-

Y

-

G

-

S

-

G

G
x
Q

L

C

L

L

N

T

E

E

Q

E

G

S

M

Y

P

-

R

-

L

V

A

A

A

N

T

K

F

I

G

F

H

K

G

G

T

F

P

G

A

T

N

N

Y

N

M

V

G

D

T

G

F
x
N

P

P

A

R

F

L

L
x
C

-

M

-

A

A

S

A

A

W

V

G

G

P

G

I

Y

D

V

F

T

S

S

F

F

G

G

S

K

G

D

Q

E

G

P

V

M

K

-

C

-

V

-

H

-

S

-

E

-

H

-

L

-

Y

-

G

G

E

T

Y

Y

W

-

H

-

R

-

A

-

F

-

T

-

V

-

A

-

A

A

D

D

T

I

P

D

N

Q

A

A

N

T

F

C

I

F

V

F

S

I

F

I

G

G

T

S

N
|
N

V
x
T

E
x
S

V

-

S
x
E

G
x
A

G

H

P

P

C

V

G

L

V

F

R

R

H

I

A

A

D

R

A

R

R

K

I

Q

R

V

-

E

-

P

G

G

I

V

K

K

R

I

V

I

Q

V

V

A

E
x
D

P
|
P

H
x
R

L

R

S

T

P

N

T

T

G

S

A

R

C

I

S

A

A

D

Q

M

W

H

V

V

P

A

I

F

R

R

P

P

K
x
G

T
|
T

D
|
D

P

L

A

A

F

F

M

M

F

H

A

S

M

M

L

A

H

W

V

V

M

I

L

I

H

N

E

E

C45 (= C12) binding
C48 (= C15) binding
C52 (≠ P19) binding
C79 (= C51) binding
K81 (= K53) binding
Q143 (≠ G109) binding
N168 (≠ F136) binding
C172 (≠ L140) binding
NTS-EA 208:212 (≠ NVEVSG 191:196) binding
DPR 236:238 (≠ EPH 218:220) binding
GTD 255:257 (≠ KTD 237:239) binding

Sites not aligning to the query:

1:32 signal peptide, Tat-type signal
340 binding
344 binding
450 binding
475:477 binding
647:656 binding
729 binding
746 binding

2jirA A new catalytic mechanism of periplasmic nitrate reductase from desulfovibrio desulfuricans atcc 27774 from crystallographic and epr data and based on detailed analysis of the sixth ligand (see paper)
29% identity, 27% coverage: 6:253/911 of query aligns to 4:236/720 of 2jirA

query
sites
2jirA

K

K

S

W

V

V

P

K

T

G

Y

V

C
|
C

Y
x
R

N

Y

C
|
C

V

G

A
x
T

G

G

P
x
C

D

G

L

V

M

L

C

-

V

V

T

G

V

V

K

K

D

D

G

G

V

K

A

A

V

V

A

A

V

I

-

Q

-

G

T

N

P

P

N

N

H

N

A

H

G

N

M

A

G

G

I

L

H
x
L

P

-

A

-

D

-

G

-

R

-

P

-

C
|
C

V

L

K
|
K

A

G

Y

S

G

L

L

L

V

I

Q

P

K

V

T

L

Y

N

N

S

P

K

H

E

R

R

V

V

L

T

T

Q

P

P

M

L

K

V

R

R

T

R

N

H

P

-

R

K

K

G

G

G

R

K

D

L

E

E

D

-

P

-

G

-

F

-

V

-

P

P

I

V

S

S

W

W

D

D

E

E

A

A

L

L

D

D

L

L

V

M

A

A

G

S

K

R

L

F

R

R

S

S

V

S

R

I

E

D

M

M

-

Y

-

G

-

P

-

N

-

S

-

V

-

A

-

W

-

Y

-

G

-
x
S

-

G

G
x
Q

L

C

L

L

N

T

E

E

Q

E

G

S

M

Y

P

-

R

-

L

V

A

A

A

N

T

K

F

I

G

F

H

K

G

G

T

F

P

G

A

T

N

N

Y

N

M

V

G

D

T

G

F

N

P

P

A

R

F

L

L
x
C

-
x
M

-

A

A

S

A

A

W

V

G

G

P

G

I

Y

D

V

F

T

S

S

F

F

G

G

S

K

G

D

Q

E

G

P

V

M

K

-

C

-

V

-

H

-

S

-

E

-

H

-

L

-

Y

-

G

G

E

T

Y

Y

W

-

H

-

R

-

A

-

F

-

T

-

V

-

A

-

A

A

D

D

T

I

P

D

N

Q

A

A

N

T

F

C

I

F

V

F

S

I

F

I

G
|
G

T
x
S

N
|
N

V

T

E

S

V

-

S
x
E

G
x
A

G

H

P
|
P

C
x
V

G

L

V

F

R

R

H

I

A

A

D

R

A

R

R

K

I

Q

R

V

-

E

-

P

G

G

I

V

K

K

R

I

V

I

Q

V

V

A

E
x
D

P
|
P

H
x
R

L

R

S

T

P

N

T

T

G

S

A

R

C

I

S

A

A

D

Q

M

W

H

V

V

P

A

I
x
F

R

R

P

P

K

G

T

T

D
|
D

P

L

A

A

F

F

M

M

F

H

A

S

M

M

L

A

H

W

V

V

M

I

L

I

H

N

E

E

active site: K46 (= K53), S106 (vs. gap), C137 (≠ L140), M138 (vs. gap), A177 (≠ G196)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: R11 (≠ Y13), K46 (= K53), Q108 (≠ G109), C137 (≠ L140), G171 (= G189), S172 (≠ T190), N173 (= N191), E176 (≠ S195), A177 (≠ G196), D201 (≠ E218), P202 (= P219), R203 (≠ H220), F217 (≠ I234), D222 (= D239)
binding iron/sulfur cluster: C10 (= C12), C13 (= C15), T15 (≠ A17), C17 (≠ P19), L43 (≠ H44), C44 (= C51), P179 (= P198), V180 (≠ C199)

Sites not aligning to the query:

active site: 305, 341, 342, 343
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 304, 305, 306, 309, 310, 342, 343, 343, 413, 414, 415, 419, 440, 441, 442, 458, 460, 612, 613, 614, 614, 616, 618, 619, 620, 620, 621, 623, 686, 694, 710, 711
binding nitrite ion: 615, 686

2jiqA A new catalytic mechanism of periplasmic nitrate reductase from desulfovibrio desulfuricans atcc 27774 from crystallographic and epr data and based on detailed analysis of the sixth ligand (see paper)
29% identity, 27% coverage: 6:253/911 of query aligns to 4:236/720 of 2jiqA

query
sites
2jiqA

K

K

S

W

V

V

P

K

T

G

Y

V

C
|
C

Y
x
R

N

Y

C
|
C

V

G

A
x
T

G

G

P
x
C

D

G

L

V

M

L

C

-

V

V

T

G

V

V

K

K

D

D

G

G

V

K

A

A

V

V

A

A

V

I

T

Q

-

G

-

D

P

P

N

N

H

N

A

H

G

N

M

A

G

G

I

L

H
x
L

P

-

A

-

D

-

G

-

R

-

P

-

C
|
C

V

L

K
|
K

A

G

Y

S

G

L

L

L

V

I

Q

P

K

V

T

L

Y

N

N

S

P

K

H

E

R

R

V

V

L

T

T

Q

P

P

M

L

K

V

R

R

T

R

N

H

P

-

R

K

K

G

G

G

R

K

D

L

E

E

D

-

P

-

G

-

F

-

V

-

P

P

I

V

S

S

W

W

D

D

E

E

A

A

L

L

D

D

L

L

V

M

A

A

G

S

K

R

L

F

R

R

S

S

V

S

R

I

E

D

M

M

-

Y

-

G

-

P

-

N

-

S

-

V

-

A

-

W

-

Y

-

G

-
x
S

-

G

G
x
Q

L

C

L

L

N

T

E

E

Q

E

G

S

M

Y

P

-

R

-

L

V

A

A

A

N

T

K

F

I

G

F

H

K

G

G

T

F

P

G

A

T

N

N

Y

N

M

V

G

D

T

G

F

N

P

P

A

R

F

L

L
x
C

-
x
M

-

A

A

S

A

A

W

V

G

G

P

G

I

Y

D

V

F

T

S

S

F

F

G

G

S

K

G

D

Q

E

G

P

V

M

K

-

C

-

V

-

H

-

S

-

E

-

H

-

L

-

Y

-

G

G

E

T

Y

Y

W

-

H

-

R

-

A

-

F

-

T

-

V

-

A

-

A

A

D

D

T

I

P

D

N

Q

A

A

N

T

F

C

I

F

V

F

S

I

F

I

G
|
G

T
x
S

N

N

V

T

E

S

V

-

S
x
E

G
x
A

G

H

P

P

C
x
V

G

L

V

F

R

R

H

I

A

A

D

R

A

R

R

K

I

Q

R

V

-

E

-

P

G

G

I

V

K

K

R

I

V

I

Q

V

V

A

E
x
D

P
|
P

H
x
R

L

R

S

T

P

N

T

T

G

S

A

R

C

I

S

A

A

D

Q

M

W

H

V

V

P

A

I

F

R

R

P
|
P

K

G

T

T

D
|
D

P

L

A

A

F

F

M

M

F

H

A

S

M

M

L

A

H

W

V

V

M

I

L

I

H

N

E

E

active site: K46 (= K53), S106 (vs. gap), C137 (≠ L140), M138 (vs. gap), A177 (≠ G196)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: R11 (≠ Y13), Q108 (≠ G109), C137 (≠ L140), G171 (= G189), S172 (≠ T190), E176 (≠ S195), A177 (≠ G196), D201 (≠ E218), P202 (= P219), R203 (≠ H220), P219 (= P236), D222 (= D239)
binding iron/sulfur cluster: C10 (= C12), C13 (= C15), T15 (≠ A17), C17 (≠ P19), L43 (≠ H44), C44 (= C51), V180 (≠ C199)

Sites not aligning to the query:

active site: 305, 341, 342, 343
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 304, 305, 306, 309, 310, 342, 343, 343, 413, 414, 415, 419, 440, 441, 443, 458, 460, 612, 613, 614, 614, 616, 618, 619, 620, 620, 621, 623, 686, 694, 710, 711
binding nitrate ion: 251, 583, 589, 591, 593, 704

2e7zA Acetylene hydratase from pelobacter acetylenicus (see paper)
23% identity, 27% coverage: 12:253/911 of query aligns to 6:232/727 of 2e7zA

query
sites
2e7zA

C
|
C

Y

Q

N
x
S

C
|
C

V

-

A

-

G

-

P

-

D
|
D

L
x
I

M
x
N

C
|
C

V

V

T

V

V

E

K

A

D

E

G

V

V

K

A

A

V

D

A

G

V

K

T

I

P

Q

N

T

H

K

A

S

G

I

M

S

G

E

I

P

H

H

P

P

A

T

D

T

-

P

G

N

R

S

P

I

C
|
C

V

M

K
|
K

A
x
S

Y

V

G

N

L

A

V

D

Q

T

K

I

T

R

Y

T

N

H

P

K

H

D

R

R

V

V

L

L

T

Y

P

P

M

L

K

K

R

N

T

V

N

G

P

S

R

K

K

R

G

G

R

E

D

Q

E

R

D

-

P

-

G

-

F

W

V

E

P

R

I

I

S

S

W

W

D

D

E

Q

A

A

L

L

D

D

L

E

V

I

A

A

G

E

K

K

L

L

R

K

S

K

V

I

R

-

E

-

M

-

G

-

L

-

L

I

N

A

E

K

Q

Y

G

G

M

P

P

E

R

S

L

L

A

G

A

V

T

S

F

Q

G
x
T

H

E

G
x
I

G
x
N

T

Q

P

Q

A

S

N

E

Y

Y

M

-

G

G

T

T

F

L

P

R

A

R

F

F

L

M

A

N

A

L

W

L

G

G

P

S

I

P

D

N

F
x
W

S

T

-

S

-
x
A

-

M

-

Y

-
x
M

-
x
C

F
x
I

G

G

S

N

G

T

Q

A

G

G

V

V

K

H

C

R

V

V

H

-

S

-

E

-

H

-

L

T

Y

H

G

G

E

S

Y

Y

W

S

H

F

R

A

A

S

F

F

T

A

V

-

A

-

D

-

T

-

P

-

N

D

A

S

N

N

F

C

I

L

V

L

S

F

F

I

G
|
G

T
x
K

N
|
N

V

L

E

S

V

N

S

H

G
x
N

G
x
W

P
x
V

C

S

G

Q

V

F

R

N

R

D

H

L

A

K

D

A

A

A

R

L

I

K

R

R

G

G

I

C

K

K

R

L

V

I

Q

V

V
x
L

E
x
D

P
|
P

H
x
R

L

R

S

T

P

K

T

V

G

A

A

E

C

M

S

A

A

D

Q

I

W

W

V

L

P

P

I

L

R

R

P
x
Y

K
x
G

T

T

D
|
D

P

A

A

A

F

L

M

F

F

L

A

G

M

M

L

I

H

N

V

V

M

I

L

I

H

N

E

E

active site: K45 (= K53), T108 (≠ G124), I110 (≠ G126), W131 (≠ F148), C138 (vs. gap), I139 (≠ F150)
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: D10 (= D20), K45 (= K53), T108 (≠ G124), N111 (≠ G127), A134 (vs. gap), M137 (vs. gap), C138 (vs. gap), G168 (= G189), K169 (≠ T190), N170 (= N191), N175 (≠ G196), W176 (≠ G197), L196 (≠ V217), D197 (≠ E218), P198 (= P219), R199 (≠ H220), Y215 (≠ P236), G216 (≠ K237), D218 (= D239)
binding iron/sulfur cluster: C6 (= C12), S8 (≠ N14), C9 (= C15), I11 (≠ L21), N12 (≠ M22), C13 (= C23), C43 (= C51), K45 (= K53), S46 (≠ A54), W176 (≠ G197), V177 (≠ P198)

Sites not aligning to the query:

active site: 292, 335, 336, 337
binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 292, 293, 296, 297, 413, 415, 419, 420, 439, 440, 441, 457, 462, 599, 602, 603, 603, 604, 605, 607, 608, 609, 609, 610, 611, 673, 696, 716, 717

Q71EW5 Acetylene hydratase; Ahy; EC 4.2.1.112 from Syntrophotalea acetylenica (Pelobacter acetylenicus) (see 3 papers)
23% identity, 27% coverage: 12:253/911 of query aligns to 9:235/730 of Q71EW5

query
sites
Q71EW5

C
|
C

Y

Q

N

S

C
|
C

V

-

A

-

G

-

P

-

D
|
D

L

I

M

N

C
|
C

V

V

T

V

V

E

K

A

D

E

G

V

V

K

A

A

V

D

A

G

V

K

T

I

P

Q

N

T

H

K

A

S

G

I

M

S

G

E

I

P

H

H

P

P

A

T

D

T

-

P

G

N

R

S

P

I

C
|
C

V

M

K
|
K

A

S

Y

V

G

N

L

A

V

D

Q

T

K

I

T

R

Y

T

N

H

P

K

H

D

R

R

V

V

L

L

T

Y

P

P

M

L

K

K

R

N

T

V

N

G

P

S

R

K

K

R

G

G

R

E

D

Q

E

R

D

-

P

-

G

-

F

W

V

E

P

R

I

I

S

S

W

W

D

D

E

Q

A

A

L

L

D

D

L

E

V

I

A

A

G

E

K

K

L

L

R

K

S

K

V

I

R

-

E

-

M

-

G

-

L

-

L

I

N

A

E

K

Q

Y

G

G

M

P

P

E

R

S

L

L

A

G

A

V

T

S

F

Q

G
x
T

H
x
E

G
x
I

G
x
N

T

Q

P

Q

A

S

N

E

Y

Y

M

-

G

G

T

T

F

L

P

R

A

R

F

F

L

M

A

N

A

L

W

L

G

G

P

S

I

P

D

N

F

W

S

T

-

S

-

A

-

M

-

Y

-

M

-
x
C

F
x
I

G

G

S

N

G

T

Q

A

G

G

V

V

K

H

C

R

V

V

H

-

S

-

E

-

H

-

L

T

Y

H

G

G

E

S

Y

Y

W

S

H

F

R

A

A

S

F

F

T

A

V

-

A

-

D

-

T

-

P

-

N

D

A

S

N

N

F

C

I

L

V

L

S

F

F

I

G

G

T
x
K

N
|
N

V

L

E

S

V

N

S
x
H

G
x
N

G
x
W

P

V

C

S

G

Q

V

F

R

N

R

D

H

L

A

K

D

A

A

A

R

L

I

K

R

R

G

G

I

C

K

K

R

L

V

I

Q

V

V
x
L

E
x
D

P
|
P

H
x
R

L

R

S

T

P

K

T

V

G

A

A

E

C

M

S

A

A

D

Q

I

W

W

V

L

P

P

I

L

R

R

P
x
Y

K
x
G

T
|
T

D
|
D

P

A

A

A

F

L

M

F

F

L

A

G

M

M

L

I

H

N

V

V

M

I

L

I

H

N

E

E

C9 (= C12) binding
C12 (= C15) binding
D13 (= D20) mutation to A: Almost abolishes catalytic activity.; mutation to E: Does not affect catalytic activity.
C16 (= C23) binding
C46 (= C51) binding
K48 (= K53) binding ; mutation to A: Does not affect catalytic activity.
TEIN 111:114 (≠ GHGG 124:127) binding
C141 (vs. gap) binding
I142 (≠ F150) mutation to A: Strongly impairs catalytic activity.
KN 172:173 (≠ TN 190:191) binding
HNW 177:179 (≠ SGG 195:197) binding
LDPR 199:202 (≠ VEPH 217:220) binding
YGTD 218:221 (≠ PKTD 236:239) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
296 binding
300 binding
416:418 binding
422:423 binding
442:444 binding
460 binding
465 binding
602:613 binding
606 binding
676 binding
699 binding
720 binding

Query Sequence

>WP_011384418.1 NCBI__GCF_000009985.1:WP_011384418.1
MSVETKSVPTYCYNCVAGPDLMCVTVKDGVAVAVTPNHAGMGIHPADGRPCVKAYGLVQK
TYNPHRVLTPMKRTNPRKGRDEDPGFVPISWDEALDLVAGKLRSVREMGLLNEQGMPRLA
ATFGHGGTPANYMGTFPAFLAAWGPIDFSFGSGQGVKCVHSEHLYGEYWHRAFTVAADTP
NANFIVSFGTNVEVSGGPCGVRRHADARIRGIKRVQVEPHLSPTGACSAQWVPIRPKTDP
AFMFAMLHVMLHEAPRSALDLDFLRDRTASPYLVDDEGWYLRDEATGKPLMWDGITGQPV
PHDHPGAVPALEGSFTVPAAVAKLPDGEMVRRENATARTAFTLLVDGMRGYTPDWAETIC
DVPAKVVRQVALEYLDNACVGQTTEVDGKVLPHRPVAVTLGKTVNNGWGAYECCWARTVL
ATLVGALEVPGGTLGTTVRLNRPYENRLKSVKPGEDGFMNAQMNSTKKGGWAEKPKGRNA
HTTLIPIVGDSSWAQALGPTHLAWMFLKDSPAEWAPPEPPDVWIAYRTNPAISFWDTAQL
SGNMAKMPFVVCFAYTMDETNHMADILLPEATDLESTQLIRAGGSKFVEQHWQHQGFVLR
QPAVAPQGESRDFTWITTELSRRVGLLESYNRGLNKGFALVPLKGEGFDFTLPEDQVHDV
DTIWDAVCKAATAGATKGQEVRGLDWMKENGFFMQPFAREDWYLHATMVEQGLRYELPYQ
ERLMRVGEELRRRLHEAGIQWWDEQLEEYQGIPHWHDVPGRWVKAVERAGAKAEDFPFWG
ITTKTMPYTTGNNAGIPLMNEVAGNLRGHGSVIINASSAAKLDIKDGDWVEVSSVVGHTR
GRAALVQGCRPDTVVIPGQFQHWKTPYAKDLNFPSLNTITQMSLELTDATGSGADVVRVA
LRRVDGPGEPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory