SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011384422.1 AMB_RS10195 4-hydroxybenzoyl-CoA reductase subunit gamma to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
88% identity, 100% coverage: 1:162/162 of query aligns to 1:161/161 of 1sb3C

query
sites
1sb3C

M

M

K

K

S

N

I

I

L

L

R

R

L

L

T

T

L

L

N

N

G

G

R

R

A

A

R

R

E

E

D

D

L

L

V

V

P

P

D

D

N

N

M

M

L

L

D

D

Y

Y

L

L

R

R

E

E

V

T

S

V

N

G

L

L

T

T

G

G

T

T

K

K

Q
|
Q

G

G

C
|
C

D

D

G

G

G
|
G

E

E

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

L

V

V

D

D

R

R

P

P

R

R

L

L

A

A

C
|
C

S

S

T

T

L

L

A

A

H

H

Q

Q

V

V

A

A

G

G

K

K

R

K

V

V

E

E

T

T

V

V

E

E

G

S

L

L

A

A

T

T

Q

Q

G

G

T

T

I

L

S

S

K

K

L

L

Q

Q

A

A

F

F

H

H

E

E

K

K

L

L

G

G

T

T

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

M

L

I

M

M

A

A

S

S

E

E

A

A

L

L

R

R

K

K

N

N

P

P

D

S

P

P

S

S

R

R

D

D

Q

E

I

I

K

K

E

A

A

A

L

L

A

A

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

V

K

K

I

I

E

K

S

S

V

V

E

E

V

T

A

A

K

A

A

A

R

R

C

L

G

-

C

C

Q

E

E

E

A

G

A

A

Q

R

binding fe2/s2 (inorganic) cluster: Q39 (= Q39), C41 (= C41), G44 (= G44), C46 (= C46), G47 (= G47), C49 (= C49), C61 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C135 (= C135), C137 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C137 (= C137)

1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
47% identity, 93% coverage: 2:152/162 of query aligns to 2:152/156 of 1ffuD

query
sites
1ffuD

K

K

S

K

I

I

L

I

R

T

L

V

T

N

L

V

N

N

G

G

R

K

A

A

R

Q

E

E

D

K

L

A

V

V

P

E

D

P

N

R

M

T

L

L

L

I

D

H

Y

F

L

L

R

R

E

E

V

E

S

L

N

N

L

L

T

T

G

G

T

A

K

H

Q

I

G

G

C
|
C

D

E

G

T

G
x
S

E
x
H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

D

V

I

D

D

D

G

R

R

P

S

R

V

L

K

A

S

C
|
C

S

T

T

H

L

L

A

A

H

V

Q

Q

V

C

A

D

G

G

K

S

R

E

V

V

E

L

T

T

V

V

E

E

G

G

L

L

A

A

T

N

Q

K

G

G

T

V

I

L

S

H

K

A

L

V

Q

Q

A

E

A

G

F

F

H

Y

E

K

K

E

L

H

G

G

T

L

Q

Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

M

L

L

M

M

A

R

S

A

E

Y

A

R

L

F

L

L

R

Q

K

E

N

N

P

P

D

N

P

P

S

T

R

E

D

A

Q

E

I

I

K

R

E

M

A

G

L

M

A

T

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

Q

K

N

I

I

I

V

E

K

S

A

V

V

E

Q

V

Y

A

A

K

R

binding fe2/s2 (inorganic) cluster: C41 (= C41), S44 (≠ G44), H45 (≠ E45), C46 (= C46), G47 (= G47), C49 (= C49), C61 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C135 (= C135), C137 (= C137)

1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
47% identity, 93% coverage: 2:152/162 of query aligns to 1:151/155 of 1ffvA

query
sites
1ffvA

K

K

S

K

I

I

L

I

R

T

L

V

T

N

L

V

N

N

G

G

R

K

A

A

R

Q

E

E

D

K

L

A

V

V

P

E

D

P

N

R

M

T

L

L

L

I

D

H

Y

F

L

L

R

R

E

E

V

E

S

L

N

N

L

L

T

T

G

G

T

A

K

H

Q
x
I

G

G

C
|
C

D

E

G

T

G
x
S

E

H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

D

V

I

D

D

D

G

R

R

P

S

R

V

L

K

A

S

C
|
C

S

T

T

H

L

L

A

A

H

V

Q

Q

V

C

A

D

G

G

K

S

R

E

V

V

E

L

T

T

V

V

E

E

G

G

L

L

A

A

T

N

Q

K

G

G

T

V

I

L

S

H

K

A

L

V

Q

Q

A

E

A

G

F

F

H

Y

E

K

K

E

L

H

G

G

T

L

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

M

L

L

M

M

A

R

S

A

E

Y

A

R

L

F

L

L

R

Q

K

E

N

N

P

P

D

N

P

P

S

T

R

E

D

A

Q

E

I

I

K

R

E

M

A

G

L

M

A

T

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

Q

K

N

I

I

I

V

E

K

S

A

V

V

E

Q

V

Y

A

A

K

R

binding fe2/s2 (inorganic) cluster: I38 (≠ Q39), C40 (= C41), S43 (≠ G44), C45 (= C46), G46 (= G47), C48 (= C49), C60 (= C61), C99 (= C100), G100 (= G101), C102 (= C103), C134 (= C135), C136 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q99), C136 (= C137)

1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
49% identity, 92% coverage: 8:156/162 of query aligns to 9:160/162 of 1t3qA

query
sites
1t3qA

T

T

L

I

N

N

G

G

R

K

A

P

R

R

E

V

D

F

L

Y

V

V

P

E

D

P

N

R

M

M

L

H

L

L

L

A

D

D

Y

A

L

L

R

R

E

E

V

V

S

V

N

G

L

L

T

T

G

G

T

T

K

K

Q
x
I

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

V

C
|
C

G
|
G

A

S

C
|
C

T

T

V

I

L

L

V

I

D

D

D

G

R

A

P

P

R

M

L
x
R

A

S

C
|
C

S

L

T

T

L

L

A

A

H

V

Q

Q

V

A

A

E

G

G

K

C

R

S

V

I

E

E

T

T

V

V

E

E

G

G

L

L

A

S

T

Q

Q

G

G

E

T

K

I

L

S

N

K

A

L

L

Q

Q

A

D

A

S

F

F

H

R

E

R

K

H

L

H

G

A

T

L

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

A

G

G

M

M

L

L

M

A

A

T

S

A

E

R

A

S

L

I

L

L

R

A

K

E

N

N

P

P

D

A

P

P

S

S

R

R

D

D

Q

E

I

V

K

R

E

E

A

V

L

M

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

E

K

T

I

I

E

D

S

A

V

I

E

T

-

D

-

P

-

A

V

V

A

A

A

E

K

A

A

A

R

R

C

R

G

G

binding fe2/s2 (inorganic) cluster: I40 (≠ Q39), C42 (= C41), E43 (≠ D42), G45 (= G44), C47 (= C46), G48 (= G47), C50 (= C49), R60 (≠ L59), C62 (= C61), C101 (= C100), G102 (= G101), C104 (= C103), C136 (= C135), C138 (= C137)
binding pterin cytosine dinucleotide: Q100 (= Q99), C138 (= C137)

3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
49% identity, 95% coverage: 2:155/162 of query aligns to 3:155/160 of 3hrdD

query
sites
3hrdD

K

K

S

I

I

T

L

I

R

N

L

L

T

N

L

L

N

N

G

G

R

E

A

A

R

R

E

S

D

I

L

V

V

T

P

E

D

P

N

N

M

K

L

R

L

L

D

D

Y

L

L

L

R

R

E

E

V

D

S

F

N

G

L

L

T

T

G

S

T

V

K

K

Q
x
E

G

G

C
|
C

D
x
S

G
x
E

G
|
G

E
|
E

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

I

V

F

D

N

D

G

R

D

P

P

R

V

L

T

A

T

C
|
C

S

C

T

M

L

L

A

A

H

G

Q

Q

V

A

A

D

G

E

K

S

R

T

V

I

E

I

T

T

V

L

E

E

G

G

L

V

A

A

T

E

Q

D

G

G

T

K

I

P

S

S

K

L

L

L

Q

Q

A

Q

A

C

F

F

H

L

E

E

K

A

L

G

G

A

T

V

Q
|
Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

M

L

I

M

L

A

T

S

A

E

K

A

A

L

L

R

D

K

K

N

N

P

P

D

D

P

P

S

T

R

D

D

E

Q

E

I

I

K

T

E

V

A

A

L

M

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

I

K

K

I

I

I

H

E

A

S

A

V

V

E

R

V

Y

A

A

A

V

K

E

A

-

R

R

C

C

binding flavin-adenine dinucleotide: E44 (≠ G43), G45 (= G44), E46 (= E45)
binding fe2/s2 (inorganic) cluster: E40 (≠ Q39), C42 (= C41), S43 (≠ D42), G45 (= G44), C47 (= C46), G48 (= G47), C50 (= C49), C62 (= C61), Q100 (= Q99), C101 (= C100), G102 (= G101), C104 (= C103), C136 (= C135), C138 (= C137)
binding pterin cytosine dinucleotide: Q100 (= Q99), C138 (= C137)

Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
49% identity, 95% coverage: 2:155/162 of query aligns to 3:155/157 of Q0QLF3

query
sites
Q0QLF3

K

K

S

I

I

T

L

I

R

N

L

L

T

N

L

L

N

N

G

G

R

E

A

A

R

R

E

S

D

I

L

V

V

T

P

E

D

P

N

N

M

K

L

R

L

L

D

D

Y

L

L

L

R

R

E

E

V

D

S

F

N

G

L

L

T

T

G

S

T

V

K

K

Q

E

G

G

C
|
C

D

S

G

E

G

G

E

E

C
|
C

G

G

A

A

C
|
C

T

T

V

V

L

I

V

F

D

N

D

G

R

D

P

P

R

V

L

T

A

T

C
|
C

S

C

T

M

L

L

A

A

H

G

Q

Q

V

A

A

D

G

E

K

S

R

T

V

I

E

I

T

T

V

L

E

E

G

G

L

V

A

A

T

E

Q

D

G

G

T

K

I

P

S

S

K

L

L

L

Q

Q

A

Q

A

C

F

F

H

L

E

E

K

A

L

G

G

A

T

V

Q

Q

C
|
C

G

G

F

Y

C
|
C

T

T

P

P

G

G

M

M

L

I

M

L

A

T

S

A

E

K

A

A

L

L

R

D

K

K

N

N

P

P

D

D

P

P

S

T

R

D

D

E

Q

E

I

I

K

T

E

V

A

A

L

M

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

I

K

K

I

I

I

H

E

A

S

A

V

V

E

R

V

Y

A

A

A

V

K

E

A

-

R

R

C

C

C42 (= C41) binding
C47 (= C46) binding
C50 (= C49) binding
C62 (= C61) binding
C101 (= C100) binding
C104 (= C103) binding
C136 (= C135) binding
C138 (= C137) binding

P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
45% identity, 99% coverage: 2:162/162 of query aligns to 3:166/166 of P19921

query
sites
P19921

K

K

S

A

I

H

L

I

R

E

L

L

T

T

L

I

N

N

G

G

R

H

A

P

R

V

E

E

D

A

L

L

V

V

P

E

D

P

N

R

M

T

L

L

L

I

D

H

Y

F

L

I

R

R

E

E

V

Q

S

Q

N

N

L

L

T

T

G

G

T

A

K

H

Q

I

G

G

C
|
C

D

D

G

T

G

S

E

H

C
|
C

G

G

A

A

C
|
C

T

T

V

V

L

D

V

L

D

D

D

G

R

M

P

S

R

V

L

K

A

S

C
|
C

S

T

T

M

L

F

A

A

H

V

Q

Q

V

A

A

N

G

G

K

A

R

S

V

I

E

T

T

T

V

I

E

E

G

G

L

M

A

A

T

A

-

P

Q

D

G

G

T

T

I

L

S

S

K

A

L

L

Q

Q

A

E

A

G

F

F

H

R

E

M

K

M

L

H

G

G

T

L

Q

Q

C
|
C

G

G

F

Y

C
|
C

T

T

P

P

G

G

M

M

L

I

M

M

A

R

S

S

E

H

A

R

L

L

R

Q

K

E

N

N

P

P

D

S

P

P

S

T

R

E

D

A

Q

E

I

I

K

R

E

F

A

G

L

I

A

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

Q

K

N

I

I

I

V

E

K

S

A

V

I

E

Q

V

Y

A

A

K

A

A

K

R

I

C

N

G

G

C

V

-

P

-

F

Q

E

E

E

A

A

Q

E

C42 (= C41) binding
C47 (= C46) binding
C50 (= C49) binding
C62 (= C61) binding
C102 (= C100) binding
C105 (= C103) binding
C137 (= C135) binding
C139 (= C137) binding

1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
46% identity, 93% coverage: 2:151/162 of query aligns to 1:151/161 of 1n5wA

query
sites
1n5wA

K

K

S

A

I

H

L

I

R

E

L

L

T

T

L

I

N

N

G

G

R

H

A

P

R

V

E

E

D

A

L

L

V

V

P

E

D

P

N

R

M

T

L

L

L

I

D

H

Y

F

L

I

R

R

E

E

V

Q

S

Q

N

N

L

L

T

T

G

G

T

A

K

H

Q
x
I

G
|
G

C
|
C

D

D

G

T

G
x
S

E
x
H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

D

V

L

D

D

D

G

R

M

P

S

R

V

L

K

A

S

C
|
C

S

T

T

M

L

F

A

A

H

V

Q

Q

V

A

A

N

G

G

K

A

R

S

V

I

E

T

T

T

V

I

E

E

G

G

L

M

A

A

T

A

-

P

Q

D

G

G

T

T

I

L

S

S

K

A

L

L

Q

Q

A

E

A

G

F

F

H

R

E

M

K

M

L

H

G

G

T

L

Q

Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

M

L

I

M

M

A

R

S

S

E

H

A

R

L

L

R

Q

K

E

N

N

P

P

D

S

P

P

S

T

R

E

D

A

Q

E

I

I

K

R

E

F

A

G

L

I

A

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

Q

K

N

I

I

I

V

E

K

S

A

V

I

E

Q

V

Y

A

A

binding flavin-adenine dinucleotide: S43 (≠ G44), H44 (≠ E45)
binding fe2/s2 (inorganic) cluster: I38 (≠ Q39), G39 (= G40), C40 (= C41), S43 (≠ G44), C45 (= C46), G46 (= G47), C48 (= C49), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C135 (= C135), C137 (= C137)

1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
46% identity, 93% coverage: 2:151/162 of query aligns to 1:151/158 of 1n5wD

query
sites
1n5wD

K

K

S

A

I

H

L

I

R

E

L

L

T

T

L

I

N

N

G

G

R

H

A

P

R

V

E

E

D

A

L

L

V

V

P

E

D

P

N

R

M

T

L

L

L

I

D

H

Y

F

L

I

R

R

E

E

V

Q

S

Q

N

N

L

L

T

T

G

G

T

A

K

H

Q

I

G

G

C
|
C

D

D

G

T

G
x
S

E
x
H

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

D

V

L

D

D

D

G

R

M

P

S

R

V

L

K

A

S

C
|
C

S

T

T

M

L

F

A

A

H

V

Q

Q

V

A

A

N

G

G

K

A

R

S

V

I

E

T

T

T

V

I

E

E

G

G

L

M

A

A

T

A

-

P

Q

D

G

G

T

T

I

L

S

S

K

A

L

L

Q

Q

A

E

A

G

F

F

H

R

E

M

K

M

L

H

G

G

T

L

Q
|
Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

M

L

I

M

M

A

R

S

S

E

H

A

R

L

L

R

Q

K

E

N

N

P

P

D

S

P

P

S

T

R

E

D

A

Q

E

I

I

K

R

E

F

A

G

L

I

A

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

Q

K

N

I

I

I

V

E

K

S

A

V

I

E

Q

V

Y

A

A

binding flavin-adenine dinucleotide: S43 (≠ G44), H44 (≠ E45)
binding fe2/s2 (inorganic) cluster: C40 (= C41), S43 (≠ G44), C45 (= C46), G46 (= G47), C48 (= C49), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C135 (= C135), C137 (= C137)
binding pterin cytosine dinucleotide: Q99 (= Q99), C137 (= C137)

4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
43% identity, 93% coverage: 2:152/162 of query aligns to 9:158/161 of 4zohC

query
sites
4zohC

K

K

S

V

I

K

L

I

R

T

L

L

T

K

L

I

N

N

G

G

R

E

A

K

R

Y

E

E

D

T

L

E

V

V

P

E

D

P

N

R

M

R

L

L

L

V

D

H

Y

V

L

L

R

R

E

E

V

L

S

G

N

-

L

F

T

T

G

G

T

V

K

H

Q

I

G

G

C
|
C

D

D

G

T

G
x
S

E

N

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

I

V

M

D

N

D

G

R

K

P

S

R

V

L

K

A

S

C
|
C

S

T

T

V

L

L

A

A

H

V

Q

E

V

A

A

D

G

G

K

A

R

E

V

I

E

L

T

T

V

V

E

E

G

G

L

L

A

A

T

K

Q

D

G

G

T

K

I

L

S

H

K

P

L

I

Q

Q

A

E

A

A

F

F

H

W

E

E

K

N

L

H

G

A

T

L

Q
|
Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

M

L

I

M

M

A

E

S

A

E

Y

A

W

L

L

R

R

K

E

N

K

P

P

D

N

P

P

S

T

R

E

D

E

Q

E

I

I

K

R

E

E

A

G

L

I

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

Q

K

N

I

I

I

V

E

K

S

A

V

I

E

K

V

A

A

A

K

E

binding fe2/s2 (inorganic) cluster: C47 (= C41), S50 (≠ G44), C52 (= C46), G53 (= G47), C55 (= C49), C67 (= C61), C106 (= C100), G107 (= G101), C109 (= C103), C141 (= C135), C143 (= C137)
binding pterin cytosine dinucleotide: Q105 (= Q99), C143 (= C137)

5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
45% identity, 91% coverage: 5:151/162 of query aligns to 5:152/157 of 5y6qA

query
sites
5y6qA

L

L

R

T

L

M

T

Q

L

V

N

N

G

G

R

Q

A

P

R

Y

E

P

D

M

L

E

V

I

P

D

D

P

N

R

M

V

L

T

L

L

D

D

Y

A

L

L

R

R

E

E

V

H

S

L

N

N

L

L

T

T

G

G

T

T

K

K

Q

K

G
|
G

C
|
C

D
|
D

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

L

V

V

D

N

D

G

R

Q

P

R

R

V

L

L

A

S

C
|
C

S

L

T

T

L

L

A

A

H

A

Q

Q

V

A

A

D

G

G

K

A

R

E

V

V

E

T

T

S

V

I

E

E

G

G

L

L

-

Q

A

S

T

A

Q

S

G

G

T

E

I

L

S

H

K

P

L

V

Q

Q

A

R

A

C

F

F

H

V

E

E

K

N

L

D

G

A

T

F

Q
|
Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

Q

L

I

M

M

A

S

S

A

E

V

A

A

L

C

L

I

R

K

K

E

N

G

P

H

D

A

P

G

S

S

R

D

D

D

Q

E

I

I

K

R

E

E

A

Y

L

M

A

S

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

G

G

A

Y

Y

V

P

K

H

I

I

E

D

S

A

V

I

E

K

V

Q

A

A

binding fe2/s2 (inorganic) cluster: G40 (= G40), C41 (= C41), D42 (= D42), G44 (= G44), C46 (= C46), G47 (= G47), C49 (= C49), C61 (= C61), C101 (= C100), G102 (= G101), C104 (= C103), C136 (= C135), C138 (= C137)
binding pterin cytosine dinucleotide: Q100 (= Q99), C138 (= C137)

5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
41% identity, 91% coverage: 5:151/162 of query aligns to 12:173/175 of 5g5gA

query
sites
5g5gA

L

L

R

T

L

L

T

K

L

V

N

N

G

G

R

K

A

T

R

E

E

Q

D

L

L

E

V

V

P

D

D

T

N

R

M

T

L

T

L

L

D

D

Y

T

L

L

R

R

E

E

V

N

S

L

N

H

L

L

T

I

G

G

T

T

K

K

Q

K

G
|
G

C
|
C

D
|
D

G

H

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

L

V

V

D

N

D

G

R

R

P

R

R

L

L

N

A

A

C
|
C

S

L

T

T

L

L

A

A

H

V

Q

M

V

H

A

Q

G

G

K

A

R

E

V

I

E

T

T

T

V

I

E

E

G

G

L

L

A

G

T

S

Q

P

G

D

T

N

I

L

S

H

K

P

L

M

Q

Q

A

A

F

F

H

I

E

K

K

H

L

D

G

G

T

F

Q

Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

S

G

G

M

Q

L

I

M

C

A

S

S

S

E

V

A

A

L

V

L

L

R

K

K

E

-

I

-

Q

-

D

-

G

-

I

-

P

-

S

-

H

-

V

-

T

-

V

-

D

-

L

-

V

-

S

N

A

P

P

D

E

P

T

S

T

R

A

D

D

Q

E

I

I

K

R

E

E

A

R

L

M

A

S

G

G

N

N

L

I

C
|
C

R

R

C
|
C

T

G

G

A

Y

Y

V

A

K

N

I

I

I

L

E

A

S

A

V

I

E

E

V

D

A

A

binding fe2/s2 (inorganic) cluster: G47 (= G40), C48 (= C41), D49 (= D42), G51 (= G44), C53 (= C46), G54 (= G47), C56 (= C49), C68 (= C61), C107 (= C100), G108 (= G101), C110 (= C103), C157 (= C135), C159 (= C137)

P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
41% identity, 91% coverage: 5:151/162 of query aligns to 63:224/229 of P77165

query
sites
P77165

L

L

R

T

L

L

T

K

L

V

N

N

G

G

R

K

A

T

R

E

E

Q

D

L

L

E

V

V

P

D

D

T

N

R

M

T

L

T

L

L

D

D

Y

T

L

L

R

R

E

E

V

N

S

L

N

H

L

L

T

I

G

G

T

T

K

K

Q

K

G

G

C
|
C

D

D

G

H

G

G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

T

V

V

L

L

V

V

D

N

D

G

R

R

P

R

R

L

L

N

A

A

C
|
C

S

L

T

T

L

L

A

A

H

V

Q

M

V

H

A

Q

G

G

K

A

R

E

V

I

E

T

T

T

V

I

E

E

G

G

L

L

A

G

T

S

Q

P

G

D

T

N

I

L

S

H

K

P

L

M

Q

Q

A

A

F

F

H

I

E

K

K

H

L

D

G

G

T

F

Q

Q

C
|
C

G

G

F

Y

C
|
C

T

T

P

S

G

G

M

Q

L

I

M

C

A

S

S

S

E

V

A

A

L

V

L

L

R

K

K

E

-

I

-

Q

-

D

-

G

-

I

-

P

-

S

-

H

-

V

-

T

-

V

-

D

-

L

-

V

-

S

N

A

P

P

D

E

P

T

S

T

R

A

D

D

Q

E

I

I

K

R

E

E

A

R

L

M

A

S

G

G

N

N

L

I

C
|
C

R

R

C
|
C

T

G

G

A

Y

Y

V

A

K

N

I

I

I

L

E

A

S

A

V

I

E

E

V

D

A

A

C99 (= C41) binding
C104 (= C46) binding
G105 (= G47) binding
C107 (= C49) binding
C119 (= C61) binding
C158 (= C100) binding
C161 (= C103) binding
C208 (= C135) binding
C210 (= C137) binding

4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 4usaA

query
sites
4usaA

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q
x
V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ Q39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 531, 532, 535, 539
binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 4us9A

query
sites
4us9A

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q
x
V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ Q39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding 3-phenylpropanal: 255, 257, 258, 752
binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 4us8A

query
sites
4us8A

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q
x
V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ Q39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 498, 531, 532, 535, 539
binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 4c7yA

query
sites
4c7yA

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q

V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 498, 531, 535, 539
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
binding hydrogen peroxide: 696, 697, 869

3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 3fc4A

query
sites
3fc4A

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q
x
V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ Q39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding 1,2-ethanediol: 535, 622, 696, 697, 869
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 3fahA

query
sites
3fahA

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q
x
V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ Q39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
40% identity, 90% coverage: 7:151/162 of query aligns to 6:153/907 of 1sijA

query
sites
1sijA

L

I

T

T

L

V

N

N

G

G

R

I

A

E

R

Q

E

N

D

L

L

F

V

V

P

D

D

A

N

E

M

A

L

L

L

S

D

D

Y

V

L

L

R

R

E

Q

V

Q

S

L

N

G

L

L

T

T

G

G

T

V

K

K

Q
x
V

G

G

C
|
C

D
x
E

G

Q

G
|
G

E

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

V

V

L

I

V

L

D

D

D

G

R

K

P

V

R

V

L
x
R

A

A

C
|
C

S

V

T

T

L

K

A

M

H

K

Q

R

V

V

A

A

-

D

G

G

K

A

R

Q

V

I

E

T

T

T

V

I

E

E

G

G

L

V

A

G

T

Q

Q

P

G

E

T

N

I

L

S

H

K

P

L

L

Q

Q

A

K

A

A

F

W

H

V

E

L

K

H

L

G

G

G

T

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

S

P

P

G

G

M

F

L

I

M

V

A

S

S

A

E

K

A

G

L

L

R

D

K

T

N

N

P

A

D

D

P

P

S

S

R

R

D

E

Q

D

I

V

K

R

E

D

A

W

L

F

A

Q

G

K

-

H

-

R

N

N

L

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

V

K

K

P

I

L

I

V

E

D

S

A

V

V

E

M

V

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ Q39), C40 (= C41), E41 (≠ D42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), R58 (≠ L59), C60 (= C61), Q99 (= Q99), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C135), C139 (= C137)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C137)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding arsenite: 535, 696, 697, 869
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

Query Sequence

>WP_011384422.1 AMB_RS10195 4-hydroxybenzoyl-CoA reductase subunit gamma
MKSILRLTLNGRAREDLVPDNMLLLDYLREVSNLTGTKQGCDGGECGACTVLVDDRPRLA
CSTLAHQVAGKRVETVEGLATQGTISKLQAAFHEKLGTQCGFCTPGMLMASEALLRKNPD
PSRDQIKEALAGNLCRCTGYVKIIESVEVAAKARCGCQEAAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory