SitesBLAST
Comparing WP_011384545.1 NCBI__GCF_000009985.1:WP_011384545.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 9 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
48% identity, 72% coverage: 13:214/279 of query aligns to 14:213/291 of 6n2oD
- binding magnesium ion: D101 (= D100), N129 (= N128), I131 (≠ V130)
- binding succinyl-coenzyme a: I57 (= I56), R62 (= R61), L134 (≠ M133), K136 (= K135)
- binding iron/sulfur cluster: W24 (= W23), C25 (= C24), C28 (= C27), C59 (= C58), C208 (= C209), T210 (= T211), F211 (= F212)
- binding thiamine diphosphate: I57 (= I56), G58 (= G57), C59 (= C58), S60 (= S59), H76 (= H75), G102 (= G101), D103 (= D102), N129 (= N128), I131 (≠ V130), G133 (= G132), L134 (≠ M133), T135 (= T134)
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
48% identity, 72% coverage: 13:214/279 of query aligns to 14:213/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (= R61), L134 (≠ M133)
- binding coenzyme a: K136 (= K135), Y150 (≠ M151)
- binding magnesium ion: D101 (= D100), N129 (= N128), I131 (≠ V130)
- binding iron/sulfur cluster: W24 (= W23), C25 (= C24), C28 (= C27), C59 (= C58), C208 (= C209), T210 (= T211), F211 (= F212)
- binding thiamine diphosphate: I57 (= I56), G58 (= G57), C59 (= C58), S60 (= S59), H76 (= H75), G102 (= G101), D103 (= D102), N129 (= N128), I131 (≠ V130), Y132 (= Y131), G133 (= G132), L134 (≠ M133), T135 (= T134)
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
48% identity, 72% coverage: 13:214/279 of query aligns to 14:213/291 of 6n2nB
- binding magnesium ion: D101 (= D100), N129 (= N128), I131 (≠ V130)
- binding iron/sulfur cluster: W24 (= W23), C25 (= C24), C28 (= C27), H30 (≠ D29), C59 (= C58), C208 (= C209), T210 (= T211), F211 (= F212)
- binding thiamine diphosphate: I57 (= I56), G58 (= G57), C59 (= C58), S60 (= S59), H76 (= H75), G100 (= G99), D101 (= D100), G102 (= G101), D103 (= D102), N129 (= N128), I131 (≠ V130), Y132 (= Y131), G133 (= G132), L134 (≠ M133), T135 (= T134)
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
43% identity, 72% coverage: 20:219/279 of query aligns to 2:205/301 of 5b46B
- binding magnesium ion: D87 (= D100), N115 (= N128), V117 (= V130)
- binding iron/sulfur cluster: W8 (= W23), C9 (= C24), C12 (= C27), C43 (= C58), C194 (= C209), T196 (= T211), Y197 (≠ F212)
- binding thiamine diphosphate: I41 (= I56), G42 (= G57), C43 (= C58), S44 (= S59), H62 (= H75), G86 (= G99), G88 (= G101), D89 (= D102), N115 (= N128), V117 (= V130), Y118 (= Y131), G119 (= G132), L120 (≠ M133), T121 (= T134)
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
43% identity, 72% coverage: 20:219/279 of query aligns to 5:208/304 of Q96XT4
- C12 (= C24) binding [4Fe-4S] cluster
- C15 (= C27) binding [4Fe-4S] cluster
- IGCS 44:47 (= IGCS 56:59) binding thiamine diphosphate
- C46 (= C58) binding [4Fe-4S] cluster
- D90 (= D100) binding Mg(2+)
- GD 91:92 (= GD 101:102) binding thiamine diphosphate
- N118 (= N128) binding Mg(2+)
- V120 (= V130) binding Mg(2+)
- GL 122:123 (≠ GM 132:133) binding thiamine diphosphate
- C197 (= C209) binding [4Fe-4S] cluster
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
41% identity, 73% coverage: 23:225/279 of query aligns to 4:199/275 of 5b47B
- binding magnesium ion: D83 (= D100), N111 (= N128)
- binding iron/sulfur cluster: C5 (= C24), C8 (= C27), C39 (= C58), C179 (= C209)
- binding thiamine diphosphate: I37 (= I56), G38 (= G57), C39 (= C58), S40 (= S59), H58 (= H75), G84 (= G101), D85 (= D102), N111 (= N128), V113 (= V130), Y114 (= Y131), L116 (≠ M133)
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
41% identity, 76% coverage: 23:235/279 of query aligns to 7:208/286 of 5b48B
- binding magnesium ion: D84 (= D100), N112 (= N128), V114 (= V130)
- binding iron/sulfur cluster: C8 (= C24), C11 (= C27), C42 (= C58), C183 (= C209), T185 (= T211)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I56), G41 (= G57), C42 (= C58), S43 (= S59), H59 (= H75), G85 (= G101), D86 (= D102), N112 (= N128), V114 (= V130), Y115 (= Y131), G116 (= G132), L117 (≠ M133)
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
36% identity, 90% coverage: 23:274/279 of query aligns to 11:277/305 of P72579
- K49 (≠ R61) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (≠ M133) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
36% identity, 90% coverage: 23:274/279 of query aligns to 11:277/305 of Q96Y68
- C12 (= C24) binding [4Fe-4S] cluster
- C15 (= C27) binding [4Fe-4S] cluster
- C46 (= C58) binding [4Fe-4S] cluster
- K49 (≠ R61) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D100) binding Mg(2+)
- GD 91:92 (= GD 101:102) binding thiamine diphosphate
- N118 (= N128) binding Mg(2+)
- V120 (= V130) binding Mg(2+)
- GL 122:123 (≠ GM 132:133) binding thiamine diphosphate
- K125 (= K135) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (≠ N185) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C209) binding [4Fe-4S] cluster
Query Sequence
>WP_011384545.1 NCBI__GCF_000009985.1:WP_011384545.1
MSSCDSTATYAAKDFKSDVKPVWCPGCGDYAVLSSITKAFADLGLKPHETSVVSGIGCSS
RIPAYTNTYGIHSIHGRALAVGQGLKLARPDLTVMVAGGDGDGFSIGGNHFLHACRRNVD
LTYIVMDNRVYGMTKGQPSPTTESDWDASAMAPPGGTGMTPFHPLAIALASGANFIARGF
SGDPNGTAQIIAEAIRHPGFSFVAIMSPCITFREEQRDWKNTMRNVNIEPTTDPAQAARR
LMTDDGFNCGILYRGNRPAYGKEKKATNAVSDLDREFTL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory