SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011384704.1 NCBI__GCF_000009985.1:WP_011384704.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 82% coverage: 2:109/132 of query aligns to 2:107/213 of 7bgmA

query
sites
7bgmA

S

N

A

A

I

V

D

D

S

S

A

-

A

-

V

L

L

L

A

D

K

S

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K

F

W

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N

D

K

G

G

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D

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T

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A

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E

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L

I

A

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Y

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H

D

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H

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G

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C

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Y

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Y

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T

binding zinc ion: D80 (= D82), D82 (= D84), D84 (= D86), C97 (= C99), C104 (= C106)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
45% identity, 81% coverage: 3:109/132 of query aligns to 1:105/204 of 7bgnA

query
sites
7bgnA

A

A

I

V

D

D

S

S

A

-

A

-

V

L

L

L

A

D

K

S

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K

F

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G

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M

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A

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I

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F

Y

S

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G

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L

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W
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W

R

T

K
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K

G
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G

E

E

T
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T

S
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S

G

N

Q

N

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F

Q

I

R

N

L

V

K

H

D

D

F

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L

D
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D

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C

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D

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D

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I

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I

L

Y

K

L

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D

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P

D

D

G

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A
x
T

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C

H
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H

T

T

G

G

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A

R

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T

T

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C

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Y

Y

Y

T

T

binding adenosine monophosphate: W60 (= W64), K62 (= K66), G63 (= G67), T65 (= T69), S66 (= S70), T94 (≠ A98), H96 (= H100)
binding zinc ion: D78 (= D82), D80 (= D84), D82 (= D86), C95 (= C99), C102 (= C106)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

6j2lA Crystal structure of bi-functional enzyme (see paper)
43% identity, 72% coverage: 18:112/132 of query aligns to 15:109/200 of 6j2lA

query
sites
6j2lA

D

D

G

G

L

L

V

M

P

P

A

V

I

I

A

V

Q

Q

Q

H

H

A

D

V

T

S

G

G

E

E

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L

M

M

M

L

A

G

W

Y

M

M

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N

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E

E

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A

D

E

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G

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L

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W

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K

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E

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G

G

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D

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N

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L

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H

binding zinc ion: D79 (= D82), D81 (= D84), D83 (= D86), C96 (= C99), C103 (= C106)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
41% identity, 72% coverage: 18:112/132 of query aligns to 14:102/185 of 6j2lB

query
sites
6j2lB

D

D

G

G

L

L

V

M

P

P

A

V

I

I

A

V

Q

Q

Q

H

H

A

D

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G

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L

M

M

M

L

A

G

W

Y

M

M

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N

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E

E

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A

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L

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D

E

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G

R

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L

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W

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K

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G

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E

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T

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S

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G

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L

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K

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F

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L

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I

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D
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D

C

C

D
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D

G

N

D
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D

T

T

I

L

L

L

L

V

K

-

V

-

D

-

Q

-

D

-

G

-

V

L

A

A

C

N

H

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T

I

G

G

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R

S

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C

F

F

Y

G

T

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A

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A

A

R

H

binding zinc ion: D78 (= D82), D80 (= D84), D82 (= D86)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_011384704.1 NCBI__GCF_000009985.1:WP_011384704.1
MSAIDSAAVLAKIKFNADGLVPAIAQQHDTGEVLMMAWMNAESVAETLATGRVCYFSRSR
GGLWRKGETSGQQQRLKDFLIDCDGDTILLKVDQDGVACHTGRRTCFYTAARPEGLTEVA
KVQVSPDELYKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory