SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011384921.1 NCBI__GCF_000009985.1:WP_011384921.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
43% identity, 96% coverage: 7:228/232 of query aligns to 1:224/232 of 1f3oA

query
sites
1f3oA

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M

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binding adenosine-5'-diphosphate: Y11 (≠ F17), G41 (= G47), G43 (= G49), K44 (= K50), S45 (= S51), T46 (= T52)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
42% identity, 96% coverage: 7:228/232 of query aligns to 1:224/230 of 1l2tA

query
sites
1l2tA

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binding adenosine-5'-triphosphate: Y11 (≠ F17), S40 (= S46), G41 (= G47), S42 (= S48), G43 (= G49), K44 (= K50), S45 (= S51), T46 (= T52), F138 (vs. gap), Q145 (= Q149), S147 (= S151), G149 (= G153), Q150 (≠ E154), H204 (= H208)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
42% identity, 96% coverage: 5:227/232 of query aligns to 3:223/233 of P75957

query
sites
P75957

Q

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L

G42 (= G44) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
42% identity, 96% coverage: 6:227/232 of query aligns to 1:220/226 of 7mdyC

query
sites
7mdyC

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L

binding adp orthovanadate: Y12 (≠ F17), G42 (= G47), S43 (= S48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), Q91 (= Q98), H138 (≠ A145), E142 (≠ Q149), S144 (= S151), G145 (= G152), G146 (= G153), E168 (= E175), N172 (≠ A179), H201 (= H208)
binding magnesium ion: S46 (= S51), Q91 (= Q98)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
42% identity, 96% coverage: 6:227/232 of query aligns to 1:220/222 of 7arlD

query
sites
7arlD

V

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N

N

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A

D

D

E

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D

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L

binding adenosine-5'-diphosphate: G42 (= G47), G44 (= G49), K45 (= K50), S46 (= S51)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
41% identity, 96% coverage: 5:227/232 of query aligns to 2:222/229 of 7v8iD

query
sites
7v8iD

Q

K

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x
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S

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T

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G

S

A

K

W

L

T

S

G

S

G

A

D

A

L

K

R

A

R

E

L

L

R

R

L

N

D

Q

K

K

I

L

G

G

F

F

I

I

F

Y

Q
|
Q

F

F

H

H

N

H

L

L

L

L

P

P

F

D

L

F

N

T

S

A

R

L

D

E

N

N

V

V

A

A

V

M

V

P

L

L

E

L

L

I

A

G

G

K

Q

K

D

K

P

P

A

A

A

E

A

I

R

N

R

S

R

R

A

A

G

L

E

E

L

M

L

L

D

K

Y

A

L

V

E

G

V

L

G

D

A

H

R
|
R

A

A

E

N

A
x
H

S

R

P

P

G

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

E
|
E

A

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

N

N

P

P

R

R

L

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

A

N

L

L

D

D

S

A

Q

R

R

N

A

A

A

D

I

S

V

I

M

F

D

Q

L

L

L

L

I

G

K

E

V

L

A

N

R

R

E

L

Q

Q

N

G

A

T

A

A

V

F

L

L

V

V

V

V

S

T

H
|
H

D

D

E

L

K

Q

I

L

Y

A

D

K

R

R

F

M

D

S

R

R

M

Q

V

L

R

E

V

M

R

R

D

D

G

G

V

R

L

L

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ A26), S43 (= S46), G44 (= G47), G46 (= G49), K47 (= K50), S48 (= S51), T49 (= T52), Q93 (= Q98), R137 (= R142), H140 (≠ A145), E144 (≠ Q149), S146 (= S151), G148 (= G153), E149 (= E154), H203 (= H208)
binding magnesium ion: S48 (= S51), Q93 (= Q98)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
41% identity, 94% coverage: 7:225/232 of query aligns to 3:218/592 of 5lj7A

query
sites
5lj7A

L

I

A

I

E

E

L

I

T

K

G

Q

I

L

A

N

K

R

H

Y

F

F

G

G

E

E

G

G

A

E

S

N

R

R

V

V

D

H

A
x
V

L

L

R

K

G

D

V

I

D

S

L

L

S

S

L

I

H

E

P

R

G

G

E

D

V

F

V

V

G

A

L

I

L

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

N

N

I

I

I

I

G

G

C

C

V

L

V

D

E

T

P

A

N

T

A

G

G

G

R

S

M

S

V

K

L

I

D

D

G

G

V

K

V

E

V

T

Y

I

D

E

G

-

A

-

W

L

T

T

G

N

G

D

D

Q

L

L

R

S

R

D

L

L

R

R

L

S

D

Q

K

K

I

F

G

G

F

F

I

I

F

F

Q
|
Q

F

R

H

Y

N

N

L

L

L

L

P

S

F

S

L

L

N

T

S

A

R

A

D

E

N

N

V

V

A

A

V

L

V

P

L

A

E

I

L

Y

A

A

G

G

Q

M

D

P

P

Q

A

S

A

Q

A

R

R

L

R

E

R

R

A

A

G

K

E

Q

L

L

L

L

D

E

Y

K

L

L

E

G

V

L

G

G

A

D

R
x
K

A

W

E

Q

A

N

S

K

P

P

G

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

A

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

N

G

P

G

R

E

L

I

I

I

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

A

A

L

L

D

D

S

S

Q

H

R

S

A

G

A

E

I

N

V

V

M

M

D

E

L

I

L

L

I

-

K

R

V

Q

A

L

R

H

E

E

Q

E

N

G

A

H

A

T

V

I

L

I

V

M

V

V

S

T

H

H

D

D

E

K

K

H

I

I

Y

A

D

A

R

S

F

A

D

N

R

R

M

I

V

I

R

E

V

I

R

K

D

D

G

G

binding adenosine-5'-triphosphate: V22 (≠ A26), S42 (= S46), G43 (= G47), G45 (= G49), K46 (= K50), S47 (= S51), T48 (= T52), Q92 (= Q98), K136 (≠ R142), Q143 (= Q149), S145 (= S151), G147 (= G153), Q148 (≠ E154)
binding magnesium ion: S47 (= S51), Q92 (= Q98)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
41% identity, 94% coverage: 7:225/232 of query aligns to 3:218/615 of 5lilA

query
sites
5lilA

L

I

A

I

E

E

L

I

T

K

G

Q

I

L

A

N

K

R

H

Y

F
|
F

G

G

E

E

G

G

A

E

S

N

R

R

V

V

D

H

A
x
V

L

L

R

K

G

D

V

I

D

S

L

L

S

S

L

I

H

E

P

R

G

G

E

D

V

F

V

V

G

A

L

I

L

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

N

N

I

I

I

I

G

G

C

C

V

L

V

D

E

T

P

A

N

T

A

G

G

G

R

S

M

S

V

K

L

I

D

D

G

G

V

K

V

E

V

T

Y

I

D

E

G

-

A

-

W

L

T

T

G

N

G

D

D

Q

L

L

R

S

R

D

L

L

R

R

L

S

D

Q

K

K

I

F

G

G

F

F

I

I

F

F

Q
|
Q

F

R

H

Y

N

N

L

L

L

L

P

S

F

S

L

L

N

T

S

A

R

A

D

E

N

N

V

V

A

A

V

L

V

P

L

A

E

I

L

Y

A

A

G

G

Q

M

D

P

P

Q

A

S

A

Q

A

R

R

L

R

E

R

R

A

A

G

K

E

Q

L

L

L

L

D

E

Y

K

L

L

E

G

V

L

G

G

A

D

R
x
K

A

W

E

Q

A

N

S

K

P

P

G

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

A

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

N

G

P

G

R

E

L

I

I

I

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

A

A

L

L

D

D

S

S

Q

H

R

S

A

G

A

E

I

N

V

V

M

M

D

E

L

I

L

L

I

R

K

Q

V

L

A

-

R

H

E

E

Q

E

N

G

A

H

A

T

V

I

L

I

V

M

V

V

S

T

H

H

D

D

E

K

K

H

I

I

Y

A

D

A

R

S

F

A

D

N

R

R

M

I

V

I

R

E

V

I

R

K

D

D

G

G

binding adenosine-5'-triphosphate: F13 (= F17), V22 (≠ A26), S42 (= S46), G43 (= G47), G45 (= G49), K46 (= K50), S47 (= S51), T48 (= T52), Q92 (= Q98), K136 (≠ R142), Q143 (= Q149), S145 (= S151), G147 (= G153), Q148 (≠ E154)
binding magnesium ion: S47 (= S51), Q92 (= Q98)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
41% identity, 97% coverage: 7:232/232 of query aligns to 3:225/226 of 5xu1B

query
sites
5xu1B

L

L

A

I

E

S

L

L

T

K

G

N

I

I

A

F

K

R

H

S

F

Y

G

R

E

N

G

G

A

D

S

Q

R

E

V

L

D

Q

A

V

L

L

R

K

G

N

V

I

D

N

L

L

S

E

L

V

H

N

P

E

G

G

E

E

V

F

V

V

G

A

L

I

L

M

G

G

P

P

S

S

G

G

S

S

G
|
G

K
|
K

S
|
S

T

T

L

L

L

M

N

N

I

T

I

I

G

G

C

M

V

L

V

D

E

T

P

P

N

T

A

S

G

G

R

E

M

Y

V

Y

L

L

D

E

G

G

V

Q

V

E

V

V

Y

-

D

-

G

A

A

G

W

L

T

G

G

E

G

K

D

Q

L

L

R

A

R

K

L

V

R

R

L

N

D

Q

K

Q

I

I

G

G

F

F

I

V

F

F

Q

Q

F

Q

H

F

N

F

L

L

L

L

P

S

F

K

L

L

N

N

S

A

R

L

D

Q

N

N

V

V

A

E

V

L

V

P

L

L

E

I

L

Y

A

A

G

G

Q

V

D

S

P

S

A

S

A

K

A

R

R

R

R

K

R

L

A

A

G

E

E

E

L

Y

L

L

D

D

Y

K

L

V

E

E

V

L

G

T

A

E

R

R

A

S

E

H

A

H

S

L

P

P

G

S

Q

E

L

L

S

S

G

G

G

G

E

Q

A

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

N

N

P

P

R

S

L

I

I

I

L

L

A

A

D

D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

S

T

Q

K

R

T

A

G

A

N

I

Q

V

I

M

M

D

Q

L

L

L

L

I

V

K

D

V

L

A

N

R

K

E

E

Q

-

N

G

A

K

A

T

V

I

L

I

V

M

V

V

S

T

H

H

D

E

E

P

K

E

I

I

Y

A

D

A

R

Y

F

A

D

K

R

R

M

Q

V

I

R

V

V

I

R

R

D

D

G

G

V

V

L

I

E

S

S

S

G

D

S

S

A

A

binding magnesium ion: G45 (= G49), K46 (= K50), S47 (= S51)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
42% identity, 95% coverage: 5:225/232 of query aligns to 2:219/650 of 5ws4A

query
sites
5ws4A

Q

Q

V

A

L

L

A

L

E

E

L

V

T

S

G

N

I

L

A

V

K

R

H

E

F

F

G

P

E

A

G

G

A

E

S

S

R

T

V

I

D

Q

A

I

L

L

R

K

G

G

V

I

D

D

L

L

S

T

L

I

H

Y

P

E

G

G

E

E

V

L

V

V

G

A

L

I

L

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

L

L

L

M

N

N

I

I

I

L

G

G

C

C

V

L

V

D

E

R

P

P

N

T

A

S

G

G

R

S

M

Y

V

K

L

V

D

N

G

G

V

Q

V

-

V

-

Y

E

D

T

G

G

A

K

W

L

T

E

G

P

G

D

D

Q

L

L

R

A

R

Q

L

L

R

R

L

R

D

E

K

Y

I

F

G

G

F

F

I

I

F

F

Q

Q

F

R

H

Y

N

H

L

L

L

L

P

G

F

D

L

L

N

S

S

A

R

E

D

G

N

N

V

V

A

E

V

V

V

P

L

A

E

V

L

Y

A

A

G

G

Q

V

D

T

P

P

A

A

A

D

A

R

R

K

R

Q

R

R

A

A

G

T

E

A

L

L

L

L

D

T

Y

E

L

L

E

G

V

L

G

G

A

T

R

K

A

T

E

Q

A

N

S

R

P

P

G

S

Q

Q

L

L

S

S

G

G

G

G

E

Q

A

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

N

G

P

G

R

D

L

V

I

I

L

L

A

A

D

D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

S

S

Q

H

R

S

A

G

A

V

I

E

V

V

M

M

D

R

L

I

L

L

I

-

K

-

V

-

A

-

R

R

E

E

Q

L

N

N

A

A

A

A

-

G

-

H

-

T

V

I

L

I

V

L

V

V

S

T

H

H

D

D

E

M

K

Q

I

V

Y

A

D

K

R

N

F

A

D

T

R

R

M

I

V

I

R

E

V

I

R

S

D

D

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S46), G44 (= G47), S48 (= S51)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
42% identity, 94% coverage: 7:225/232 of query aligns to 4:219/648 of P75831

query
sites
P75831

L

L

A

L

E

E

L

L

T

K

G

D

I

I

A

R

K

R

H

S

F

Y

G

P

E

A

G

G

A

D

S

E

R

Q

V

V

D

E

A

V

L

L

R

K

G

G

V

I

D

S

L

L

S

D

L

I

H

Y

P

A

G

G

E

E

V

M

V

V

G

A

L

I

L

V

G

G

P

A

S

S

G

G

S

S

G

G

K
|
K

S

S

T

T

L

L

L

M

N

N

I

I

I

L

G

G

C

C

V

L

V

D

E

K

P

A

N

T

A

S

G

G

R

T

M

Y

V

R

L

V

D

A

G

G

V

-

V

-

V

-

Y

Q

D

D

G

V

A

A

W

T

T

L

G

D

G

A

D

D

-

A

L

L

R

A

R

Q

L

L

R

R

L

R

D

E

K

H

I

F

G

G

F

F

I

I

F

F

Q

Q

F

R

H

Y

N

H

L

L

L

L

P

S

F

H

L

L

N

T

S

A

R

E

D

Q

N

N

V

V

A

E

V

V

V

P

L

A

E

V

L

Y

A

A

G

G

Q

L

D

E

P

R

A

K

A

Q

A

R

R

L

R

L

R

R

A

A

G

Q

E

E

L

L

L

L

D

Q

Y

R

L

L

E

G

V

L

G

E

A

D

R

R

A

T

E

E

A

Y

S

Y

P

P

G

A

Q

Q

L

L

S

S

G

G

G

G

E

Q

A

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

N

G

P

G

R

Q

L

V

I

I

L

L

A

A

D
|
D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

S

S

Q

H

R

S

A

G

A

E

I

E

V

V

M

M

D

A

L

I

L

L

I

H

K

Q

V

L

A

-

R

R

E

D

Q

R

N

G

A

H

A

T

V

V

L

I

V

I

V

V

S

T

H

H

D

D

E

P

K

Q

I

V

Y

A

D

A

R

Q

F

A

D

E

R

R

M

V

V

I

R

E

V

I

R

R

D

D

G

G

K47 (= K50) mutation to L: Lack of activity.
D169 (= D174) mutation to N: Lack of activity.

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
42% identity, 87% coverage: 25:225/232 of query aligns to 17:214/223 of 2pclA

query
sites
2pclA

D

E

A

I

L

L

R

K

G

G

V

I

D

S

L

L

S

S

L

V

H

K

P

K

G

G

E

E

V

F

V

V

G

S

L

I

L

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

L

L

N

Y

I

I

I

L

G

G

C

L

V

L

V

D

E

A

P

P

N

T

A

E

G

G

R

K

M

V

V

F

L

L

D

E

G

G

V

K

V

E

V

V

Y

-

D

-

G

-

A

D

W

Y

T

T

G

N

-

E

G

K

D

E

L

L

R

S

R

L

L

L

R

R

L

N

D

R

K

K

I

L

G

G

F

F

I

V

F

F

Q

Q

F

F

H

H

N

Y

L

L

L

I

P

P

F

E

L

L

N

T

S

A

R

L

D

E

N

N

V

V

A

I

V

V

V

P

L

M

E

L

L

K

A

M

G

G

Q

K

D

P

P

K

A

K

A

E

A

A

R

K

R

E

R

R

A

G

G

E

E

Y

L

L

L

L

D

S

Y

E

L

L

E

G

V

L

G

G

A

D

R

K

A

L

E

S

A

R

S

K

P

P

G

Y

Q

E

L

L

S

S

G

G

G

G

E

E

A

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

N

N

E

P

P

R

I

L

L

I

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

A

G

A

N

L

L

D

D

S

S

Q

A

R

N

A

T

A

K

I

R

V

V

M

M

D

D

L

I

L

F

I

L

K

K

V

I

A

-

R

N

E

E

Q

G

N

G

A

T

A

S

V

I

L

V

V

M

V

V

S
x
T

H

H

D

E

E

R

K

E

I

L

Y

A

D

E

R

L

F

T

D

H

R

R

M

T

V

L

R

E

V

M

R

K

D

D

G

G

binding magnesium ion: E165 (= E175), P166 (= P176), T167 (= T177), T196 (≠ S207)

7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
41% identity, 90% coverage: 17:225/232 of query aligns to 14:213/218 of 7w78A

query
sites
7w78A

F
x
Y

G

P

E

D

G

G

A

I

S

S

R

T

V

V

D

T

A

A

L

L

R

D

G

S

V

A

D

N

L

V

S

E

L

I

H

F

P

P

G

G

E

E

V

L

V

V

G

A

L

I

L

V

G

G

P

E

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

S

I

I

I

A

G

G

C

F

V

L

V

Q

E

E

P

P

N

T

A

S

G

G

R

T

M

V

V

T

L

L

D

H

G

G

V

A

V

E

V

G

Y

L

D

D

G

A

A

T

W

S

T

T

G

-

G

-

D

-

L

-

R

-

R

-

L

-

R

R

L

R

D

E

K

H

I

I

G

G

F

F

I

V

F

F

Q
|
Q

F

Q

H

P

N

N

L

L

L

L

P

G

F

S

L

L

N

T

S

A

R

R

D

E

N

Q

V

L

A

L

V

I

V

T

L

D

E

H

L

L

A

R

G

G

Q

I

D

K

P

P

A

R

A

-

A

-

R

K

R

D

R

R

A

A

G

D

E

E

L

L

L

L

D

A

Y

R

L

V

E

G

V

L

G

K

A

G

R

L

A

G

E

G

A

R

S

R

P

V

G

A

Q

Q

L

L

S

S

G

G

G

G

E

Q

A

R

Q

Q

R

R

V

V

A

N

I

I

A

A

R

R

A

A

L

L

A

M

N

G

N

N

P

P

R

Q

L

L

I

L

L

L

A

A

D

D

E
|
E

P

P

T

T

A

S

A

A

L

L

D

D

S

A

Q

R

R

L

A

S

A

K

I

E

V

I

M

V

D

E

L

L

L

L

I

R

K

D

V

V

A

T

R

K

E

E

Q

F

N

A

A

L

A

A

V

T

L

L

V

M

V

V

S

T

H

H

D

D

E

R

K

S

I

Q

Y

L

D

A

R

Y

F

A

D

D

R

R

M

F

V

V

R

E

V

M

R

A

D

D

G

G

binding phosphoaminophosphonic acid-adenylate ester: Y14 (≠ F17), S43 (= S46), G44 (= G47), G46 (= G49), K47 (= K50), S48 (= S51), T49 (= T52), Q88 (= Q98), E163 (= E175)
binding magnesium ion: S48 (= S51), Q88 (= Q98)

7w79A Heme exporter hrtba in complex with mn-amppnp (see paper)
41% identity, 90% coverage: 17:225/232 of query aligns to 14:213/216 of 7w79A

query
sites
7w79A

F
x
Y

G

P

E

D

G

G

A

I

S

S

R

T

V

V

D

T

A

A

L

L

R

D

G

S

V

A

D

N

L

V

S
x
E

L

I

H

F

P

P

G

G

E

E

V

L

V

V

G

A

L

I

L

V

G

G

P

E

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

S

I

I

I

A

G

G

C

F

V

L

V

Q

E

E

P

P

N

T

A

S

G

G

R

T

M

V

V

T

L

L

D

H

G

G

V

A

V

E

V

G

Y

L

D

D

G

A

A

T

W

S

T

T

G

-

G

-

D

-

L

-

R

-

R

-

L

-

R

R

L

R

D

E

K

H

I

I

G

G

F

F

I

V

F

F

Q
|
Q

F

Q

H

P

N

N

L

L

L

L

P

G

F

S

L

L

N

T

S

A

R

R

D

E

N

Q

V

L

A

L

V

I

V

T

L

D

E

H

L

L

A

R

G

G

Q

I

D

K

P

P

A

R

A

-

A

-

R

K

R

D

R

R

A

A

G

D

E

E

L

L

L

L

D

A

Y

R

L

V

E

G

V

L

G

K

A

G

R

L

A

G

E

G

A

R

S

R

P

V

G

A

Q

Q

L

L

S

S

G

G

G

G

E

Q

A

R

Q

Q

R

R

V

V

A

N

I

I

A

A

R

R

A

A

L

L

A

M

N

G

N

N

P

P

R

Q

L

L

I

L

L

L

A

A

D

D

E
|
E

P

P

T

T

A

S

A

A

L

L

D

D

S

A

Q

R

R

L

A

S

A

K

I

E

V

I

M

V

D

E

L

L

L

L

I

R

K

D

V

V

A

T

R

K

E

E

Q

F

N

A

A

L

A

A

V

T

L

L

V

M

V

V

S

T

H
|
H

D

D

E

R

K

S

I

Q

Y

L

D

A

R

Y

F

A

D

D

R

R

M

F

V

V

R

E

V

M

R

A

D

D

G

G

binding adenosine-5'-triphosphate: Y14 (≠ F17), S43 (= S46), G44 (= G47), S45 (= S48), G46 (= G49), K47 (= K50), S48 (= S51), T49 (= T52)
binding manganese (ii) ion: E30 (≠ S33), S48 (= S51), Q88 (= Q98), E163 (= E175), H196 (= H208)

Sites not aligning to the query:

binding manganese (ii) ion: 6

8g4cB Bceabs atpgs high res tm (see paper)
31% identity, 96% coverage: 5:227/232 of query aligns to 1:221/248 of 8g4cB

query
sites
8g4cB

Q

H

V

V

L

I

A

L

E

E

L

A

T

N

G

K

I

I

A

R

K

K

H

S

F
x
Y

G

G

E

N

G

K

A

L

S

N

R

K

V

Q

D

E

A

V

L

L

R

K

G

G

V

I

D

D

L

I

S

H

L

I

H

E

P

K

G

G

E

E

V

F

V

V

G

S

L

I

L

M

G

G

P

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

L

L

L

L

N

N

I

V

I

L

G

S

C

S

V

I

V

D

E

Q

P

V

N

S

A

H

G

G

R

T

M

I

V

H

L

I

D

N

G

G

V

N

V

D

V

M

Y

-

D

-

G

T

A

A

W

M

T

K

G

E

G

K

D

Q

L

L

R

A

R

E

L

F

R

R

L

K

D

Q

K

H

I

L

G

G

F

F

I

I

F

F

Q

Q

F

D

H

Y

N

N

L

L

L

L

P

D

F

T

L

L

N

T

S

V

R

K

D

E

N

N

V

I

A

L

V

L

V

P

L

L

E

S

L

I

A

T

G

K

Q

L

D

S

P

K

A

K

A

E

A

A

R

N

R

R

R

K

A

F

G

E

E

E

L

V

L

A

D

K

Y

E

L

L

E

G

V

I

G

Y

A

E

R

L

A

R

E

D

A

K

S

Y

P

P

G

N

Q

E

L

I

S

S

G

G

G

G

E

Q

A

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

A

I

N

H

N

D

P

P

R

S

L

I

I

I

L

F

A

A

D
|
D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

S

S

Q

K

R

S

A

A

A

S

I

D

V

L

M

L

D

N

L

K

L

L

I

S

K

Q

V

L

A

N

R

Q

E

K

Q

R

N

N

A

A

A

T

V

I

L

I

V

M

V

V

S

T

H

H

D

D

E

P

K

V

I

A

Y

A

D

S

R

Y

F

C

D

G

R

R

M

V

V

I

R

F

V

I

R

K

D

D

G

G

V

Q

L

M

binding phosphothiophosphoric acid-adenylate ester: Y13 (≠ F17), S42 (= S46), S44 (= S48), T47 (≠ S51), D168 (= D174)

7tchB Bceab e169q variant atp-bound conformation (see paper)
31% identity, 96% coverage: 6:227/232 of query aligns to 1:220/245 of 7tchB

query
sites
7tchB

V

V

L

I

A

L

E

E

L

A

T

N

G

K

I

I

A

R

K

K

H

S

F
x
Y

G

G

E

N

G

K

A

L

S

N

R

K

V
x
Q

D

E

A
x
V

L

L

R

K

G

G

V

I

D

D

L

I

S

H

L

I

H

E

P

K

G

G

E

E

V

F

V

V

G

S

L

I

L

M

G

G

P

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

L

L

L

L

N

N

I

V

I

L

G

S

C

S

V

I

V

D

E

Q

P

V

N

S

A

H

G

G

R

T

M

I

V

H

L

I

D

N

G

G

V

N

V

D

V

M

Y

-

D

-

G

T

A

A

W

M

T

K

G

E

G

K

D

Q

L

L

R

A

R

E

L

F

R

R

L

K

D

Q

K

H

I

L

G

G

F

F

I

I

F

F

Q

Q

F

D

H

Y

N

N

L

L

L

L

P

D

F

T

L

L

N

T

S

V

R

K

D

E

N

N

V

I

A

L

V

L

V

P

L

L

E

S

L

I

A

T

G

K

Q

L

D

S

P

K

A

K

A

E

A

A

R

N

R

R

R

K

A

F

G

E

E

E

L

V

L

A

D

K

Y

E

L

L

E

G

V

I

G

Y

A

E

R

L

A

R

E

D

A

K

S

Y

P

P

G

N

Q
x
E

L

I

S

S

G
|
G

G
|
G

E

Q

A

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

A

I

N

H

N

D

P

P

R

S

L

I

I

I

L

F

A

A

D

D

E

Q

P

P

T

T

A

G

A

A

L

L

D

D

S

S

Q

K

R

S

A

A

A

S

I

D

V

L

M

L

D

N

L

K

L

L

I

S

K

Q

V

L

A

N

R

Q

E

K

Q

R

N

N

A

A

A

T

V

I

L

I

V

M

V

V

S

T

H

H

D

D

E

P

K

V

I

A

Y

A

D

S

R

Y

F

C

D

G

R

R

M

V

V

I

R

F

V

I

R

K

D

D

G

G

V

Q

L

M

binding adenosine-5'-triphosphate: Y12 (≠ F17), Q19 (≠ V24), V21 (≠ A26), S41 (= S46), G42 (= G47), S43 (= S48), K45 (= K50), T46 (≠ S51), E142 (≠ Q149), G145 (= G152), G146 (= G153)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 93% coverage: 7:222/232 of query aligns to 1:213/343 of P30750

query
sites
P30750

L

M

A

I

E

K

L

L

T

S

G

N

I

I

A

T

K

K

H

V

F

F

G

H

E

Q

G

G

A

T

S

R

R

T

V

I

D

Q

A

A

L

L

R

N

G

N

V

V

D

S

L

L

S

H

L

V

H

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

N

R

I

C

I

V

G

N

C

L

V

L

V

E

E

R

P

P

N

T

A

E

G

G

R

S

M

V

V

L

L

V

D

D

G

G

V

Q

V

E

V

L

Y

-

D

-

G

T

A

T

W

L

T

S

G

E

G

S

D

E

L

L

R

T

R

K

L

A

R

R

L

-

D

R

K

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

F

H

H

F

N

N

L

L

L

L

P

S

F

S

L

R

N

T

S

V

R

F

D

G

N

N

V

V

A

A

V

L

V

P

L

L

E

E

L

L

A

D

G

N

Q

T

D

P

P

K

A

D

A

E

A

V

R

K

R

R

R

R

A

V

G

T

E

E

L

L

L

L

D

S

Y

L

L

V

E

G

V

L

G

G

A

D

R

K

A

H

E

D

A

S

S

Y

P

P

G

S

Q

N

L

L

S

S

G

G

G

G

E

Q

A

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

R

K

L

V

I

L

L

L

A

C

D

D

E
|
E

P

A

T

T

A

S

A

A

L

L

D

D

S

P

Q

A

R

T

A

T

A

R

I

S

V

I

M

L

D

E

L

L

L

L

I

K

K

D

V

I

A

N

R

R

E

R

Q

L

N

G

A

L

A

T

V

I

L

L

V

L

V

I

S

T

H

H

D

E

E

M

K

D

I

V

Y

V

D

K

R

R

F

I

D

C

R

D

M

C

V

V

R

A

V

V

40:46 (vs. 46:52, 86% identical) binding
E166 (= E175) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
35% identity, 93% coverage: 7:222/232 of query aligns to 2:214/344 of 3tuzC

query
sites
3tuzC

L

M

A

I

E

K

L

L

T

S

G

N

I

I

A

T

K

K

H

V

F
|
F

G

H

E
x
Q

G

G

A

T

S

R

R

T

V

I

D

Q

A

A

L

L

R

N

G

N

V

V

D

S

L

L

S

H

L

V

H

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

P

A

S

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

N

R

I

C

I

V

G

N

C

L

V

L

V

E

E

R

P

P

N

T

A

E

G

G

R

S

M

V

V

L

L

V

D

D

G

G

V

Q

V

E

V

L

Y

-

D

-

G

T

A

T

W

L

T

S

G

E

G

S

D

E

L

L

R

T

R

K

L

A

R

R

L

-

D

R

K

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

F

H

H

F

N

N

L

L

L

L

P

S

F

S

L

R

N

T

S

V

R

F

D

G

N

N

V

V

A

A

V

L

V

P

L

L

E

E

L

L

A

D

G

N

Q

T

D

P

P

K

A

D

A

E

A

V

R

K

R

R

R

R

A

V

G

T

E

E

L

L

L

L

D

S

Y

L

L

V

E

G

V

L

G

G

A

D

R

K

A

H

E

D

A

S

S

Y

P

P

G

S

Q

N

L

L

S

S

G

G

G

G

E

Q

A

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

R

K

L

V

I

L

L

L

A

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

D

D

S

P

Q

A

R

T

A

T

A

R

I

S

V

I

M

L

D

E

L

L

L

L

I

K

K

D

V

I

A

N

R

R

E

R

Q

L

N

G

A

L

A

T

V

I

L

L

V

L

V

I

S

T

H

H

D

E

E

M

K

D

I

V

Y

V

D

K

R

R

F

I

D

C

R

D

M

C

V

V

R

A

V

V

binding adenosine-5'-diphosphate: F12 (= F17), Q14 (≠ E19), G42 (= G47), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
35% identity, 93% coverage: 7:222/232 of query aligns to 2:214/344 of 3tuiC

query
sites
3tuiC

L

M

A

I

E

K

L

L

T

S

G

N

I

I

A

T

K

K

H

V

F
|
F

G

H

E

Q

G

G

A

T

S

R

R

T

V

I

D

Q

A

A

L

L

R

N

G

N

V

V

D

S

L

L

S

H

L

V

H

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

P

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

N

R

I

C

I

V

G

N

C

L

V

L

V

E

E

R

P

P

N

T

A

E

G

G

R

S

M

V

V

L

L

V

D

D

G

G

V

Q

V

E

V

L

Y

-

D

-

G

T

A

T

W

L

T

S

G

E

G

S

D

E

L

L

R

T

R

K

L

A

R

R

L

-

D

R

K

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

F

H

H

F

N

N

L

L

L

L

P

S

F

S

L

R

N

T

S

V

R

F

D

G

N

N

V

V

A

A

V

L

V

P

L

L

E

E

L

L

A

D

G

N

Q

T

D

P

P

K

A

D

A

E

A

V

R

K

R

R

R

R

A

V

G

T

E

E

L

L

L

L

D

S

Y

L

L

V

E

G

V

L

G

G

A

D

R

K

A

H

E

D

A

S

S

Y

P

P

G

S

Q

N

L

L

S

S

G

G

G

G

E

Q

A

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

R

K

L

V

I

L

L

L

A

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

D

D

S

P

Q

A

R

T

A

T

A

R

I

S

V

I

M

L

D

E

L

L

L

L

I

K

K

D

V

I

A

N

R

R

E

R

Q

L

N

G

A

L

A

T

V

I

L

L

V

L

V

I

S

T

H

H

D

E

E

M

K

D

I

V

Y

V

D

K

R

R

F

I

D

C

R

D

M

C

V

V

R

A

V

V

binding adenosine-5'-diphosphate: F12 (= F17), G42 (= G47), A43 (≠ S48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
35% identity, 93% coverage: 7:222/232 of query aligns to 2:214/344 of 6cvlD

query
sites
6cvlD

L

M

A

I

E

K

L

L

T

S

G

N

I

I

A

T

K

K

H

V

F
|
F

G

H

E
x
Q

G

G

A

T

S

R

R

T

V
x
I

D

Q

A

A

L

L

R

N

G

N

V

V

D

S

L

L

S

H

L

V

H

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

L

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

N

R

I

C

I

V

G

N

C

L

V

L

V

E

E

R

P

P

N

T

A

E

G

G

R

S

M

V

V

L

L

V

D

D

G

G

V

Q

V

E

V

L

Y

-

D

-

G

T

A

T

W

L

T

S

G

E

G

S

D

E

L

L

R

T

R

K

L

A

R

R

L

-

D

R

K

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

F

H

H

F

N

N

L

L

L

L

P

S

F

S

L

R

N

T

S

V

R

F

D

G

N

N

V

V

A

A

V

L

V

P

L

L

E

E

L

L

A

D

G

N

Q

T

D

P

P

K

A

D

A

E

A

V

R

K

R

R

R

R

A

V

G

T

E

E

L

L

L

L

D

S

Y

L

L

V

E

G

V

L

G

G

A

D

R

K

A

H

E

D

A

S

S

Y

P

P

G

S

Q
x
N

L

L

S
|
S

G

G

G

G

E
x
Q

A

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

R

K

L

V

I

L

L

L

A

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

D

D

S

P

Q

A

R

T

A

T

A

R

I

S

V

I

M

L

D

E

L

L

L

L

I

K

K

D

V

I

A

N

R

R

E

R

Q

L

N

G

A

L

A

T

V

I

L

L

V

L

V

I

S

T

H
|
H

D

E

E

M

K

D

I

V

Y

V

D

K

R

R

F

I

D

C

R

D

M

C

V

V

R

A

V

V

binding phosphothiophosphoric acid-adenylate ester: F12 (= F17), Q14 (≠ E19), I19 (≠ V24), S41 (= S46), G42 (= G47), A43 (≠ S48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), N141 (≠ Q149), S143 (= S151), Q146 (≠ E154), H200 (= H208)

Query Sequence

>WP_011384921.1 NCBI__GCF_000009985.1:WP_011384921.1
MTGPQVLAELTGIAKHFGEGASRVDALRGVDLSLHPGEVVGLLGPSGSGKSTLLNIIGCV
VEPNAGRMVLDGVVVYDGAWTGGDLRRLRLDKIGFIFQFHNLLPFLNSRDNVAVVLELAG
QDPAAARRRAGELLDYLEVGARAEASPGQLSGGEAQRVAIARALANNPRLILADEPTAAL
DSQRAAIVMDLLIKVAREQNAAVLVVSHDEKIYDRFDRMVRVRDGVLESGSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory