SitesBLAST
Comparing WP_011384942.1 NCBI__GCF_000009985.1:WP_011384942.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
41% identity, 99% coverage: 4:351/352 of query aligns to 6:355/362 of 3bptA
- active site: G67 (= G65), P84 (≠ H83), R88 (= R87), G115 (= G114), G118 (≠ R117), E138 (= E137), D146 (= D145)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G64), G67 (= G65), I69 (= I67), E90 (= E89), G114 (= G113), G115 (= G114), E138 (= E137), D146 (= D145), V147 (= V146)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ V23), L26 (= L24), A28 (= A26), G66 (= G64), G67 (= G65), I69 (= I67), P137 (= P136), I141 (= I140), L319 (≠ V315)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
40% identity, 100% coverage: 2:352/352 of query aligns to 8:365/378 of Q9LKJ1
- G70 (= G65) mutation to S: Loss of activity.
- E142 (= E137) mutation to A: Loss of activity.
- D150 (= D145) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
38% identity, 98% coverage: 4:349/352 of query aligns to 3:335/340 of 4hdtA
- active site: G64 (= G65), I69 (≠ V70), W84 (≠ V85), Y88 (= Y90), G112 (= G114), G115 (≠ R117), E135 (= E137), P142 (= P144), D143 (= D145), R283 (= R292)
- binding zinc ion: H28 (≠ A29), E42 (= E43), E57 (= E58), E79 (= E80), H93 (≠ R95), H185 (≠ A187)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
30% identity, 64% coverage: 1:224/352 of query aligns to 1:224/259 of 5zaiC
- active site: A65 (≠ G65), F70 (≠ V70), S82 (= S82), R86 (≠ Y90), G110 (= G114), E113 (≠ R117), P132 (= P136), E133 (= E137), I138 (≠ F142), P140 (= P144), G141 (≠ D145)
- binding coenzyme a: K24 (≠ V23), L25 (= L24), A63 (= A63), G64 (= G64), A65 (≠ G65), D66 (= D66), I67 (= I67), P132 (= P136), R166 (≠ Q169)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 63% coverage: 6:226/352 of query aligns to 6:219/250 of 3q0gD
- active site: A64 (≠ G65), M69 (= M69), T75 (≠ S82), F79 (= F86), G103 (= G114), E106 (≠ R117), P125 (= P136), E126 (= E137), V131 (≠ F142), P133 (= P144), G134 (≠ D145), L219 (= L226)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
31% identity, 56% coverage: 6:203/352 of query aligns to 7:195/255 of 3q0jC
- active site: A65 (≠ G65), M70 (≠ V70), T80 (≠ S82), F84 (= F86), G108 (= G114), E111 (≠ R117), P130 (= P136), E131 (= E137), V136 (≠ F142), P138 (= P144), G139 (≠ D145)
- binding acetoacetyl-coenzyme a: Q23 (≠ K22), A24 (≠ V23), L25 (= L24), A27 (= A26), A63 (= A63), G64 (= G64), A65 (≠ G65), D66 (= D66), I67 (= I67), K68 (≠ R68), M70 (≠ V70), F84 (= F86), G107 (= G113), G108 (= G114), E111 (≠ R117), P130 (= P136), E131 (= E137), P138 (= P144), G139 (≠ D145), M140 (≠ V146)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 56% coverage: 6:203/352 of query aligns to 7:195/255 of 3q0gC
- active site: A65 (≠ G65), M70 (≠ V70), T80 (≠ S82), F84 (= F86), G108 (= G114), E111 (≠ R117), P130 (= P136), E131 (= E137), V136 (≠ F142), P138 (= P144), G139 (≠ D145)
- binding coenzyme a: L25 (= L24), A63 (= A63), I67 (= I67), K68 (≠ R68), Y104 (≠ I110), P130 (= P136), E131 (= E137), L134 (≠ I140)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
31% identity, 56% coverage: 6:203/352 of query aligns to 6:194/256 of 3h81A
- active site: A64 (≠ G65), M69 (≠ V70), T79 (≠ S82), F83 (= F86), G107 (= G114), E110 (≠ R117), P129 (= P136), E130 (= E137), V135 (≠ F142), P137 (= P144), G138 (≠ D145)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 60% coverage: 13:224/352 of query aligns to 16:219/254 of 2dubA
- active site: A67 (≠ G65), M72 (≠ F81), S82 (≠ R91), G105 (= G114), E108 (≠ R117), P127 (= P136), E128 (= E137), T133 (≠ F142), P135 (= P144), G136 (≠ D145)
- binding octanoyl-coenzyme a: K25 (= K22), A26 (≠ V23), L27 (= L24), A29 (= A26), A65 (= A63), A67 (≠ G65), D68 (= D66), I69 (= I67), K70 (≠ R68), G105 (= G114), E108 (≠ R117), P127 (= P136), E128 (= E137), G136 (≠ D145), A137 (≠ V146)
Sites not aligning to the query:
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 60% coverage: 13:224/352 of query aligns to 15:223/258 of 1ey3A
- active site: A66 (≠ G65), M71 (≠ V70), S81 (≠ H83), L85 (≠ R87), G109 (= G114), E112 (≠ R117), P131 (= P136), E132 (= E137), T137 (≠ F142), P139 (= P144), G140 (≠ D145)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K22), L26 (= L24), A28 (= A26), A64 (= A63), G65 (= G64), A66 (≠ G65), D67 (= D66), I68 (= I67), L85 (≠ R87), W88 (≠ Y90), G109 (= G114), P131 (= P136), L135 (≠ I140), G140 (≠ D145)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 60% coverage: 13:224/352 of query aligns to 17:223/258 of 1mj3A
- active site: A68 (≠ G65), M73 (≠ F81), S83 (≠ R91), L85 (= L92), G109 (= G114), E112 (≠ R117), P131 (= P136), E132 (= E137), T137 (≠ F142), P139 (= P144), G140 (≠ D145)
- binding hexanoyl-coenzyme a: K26 (= K22), A27 (≠ V23), L28 (= L24), A30 (= A26), A66 (= A63), G67 (= G64), A68 (≠ G65), D69 (= D66), I70 (= I67), G109 (= G114), P131 (= P136), E132 (= E137), L135 (≠ I140), G140 (≠ D145)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 60% coverage: 13:224/352 of query aligns to 17:225/260 of 1dubA
- active site: A68 (≠ G65), M73 (≠ V70), S83 (≠ H83), L87 (≠ R87), G111 (= G114), E114 (≠ R117), P133 (= P136), E134 (= E137), T139 (≠ F142), P141 (= P144), G142 (≠ D145)
- binding acetoacetyl-coenzyme a: K26 (= K22), A27 (≠ V23), L28 (= L24), A30 (= A26), A66 (= A63), A68 (≠ G65), D69 (= D66), I70 (= I67), Y107 (≠ I110), G110 (= G113), G111 (= G114), E114 (≠ R117), P133 (= P136), E134 (= E137), L137 (≠ I140), G142 (≠ D145)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
30% identity, 60% coverage: 13:224/352 of query aligns to 47:255/290 of P14604
- E144 (≠ R117) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E137) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
29% identity, 61% coverage: 13:225/352 of query aligns to 17:226/260 of 2hw5C
- active site: A68 (≠ G65), M73 (≠ V70), S83 (≠ R87), L87 (≠ R91), G111 (= G114), E114 (≠ R117), P133 (= P136), E134 (= E137), T139 (≠ F142), P141 (= P144), G142 (≠ D145)
- binding crotonyl coenzyme a: K26 (= K22), A27 (≠ V23), L28 (= L24), A30 (= A26), K62 (≠ R59), I70 (= I67), F109 (≠ M112)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
27% identity, 54% coverage: 25:213/352 of query aligns to 44:232/1413 of 6z1pBI
- active site: T85 (≠ G65), S134 (≠ G114), E157 (= E137), D165 (= D145)
- binding : T45 (≠ A26), D47 (≠ S28), H49 (≠ R30), K83 (≠ A63), T85 (≠ G65), D86 (= D66), F87 (≠ I67), K88 (≠ R68), K92 (≠ D72), L130 (≠ I110), K152 (≠ R132)
Sites not aligning to the query:
- binding : 4, 5, 12, 14, 41, 42, 43, 427, 731, 733, 734
3p85A Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
30% identity, 59% coverage: 12:219/352 of query aligns to 10:197/224 of 3p85A
- active site: L62 (≠ G65), L67 (≠ V70), P68 (≠ W71), G92 (= G114), E95 (≠ R117), T114 (≠ P136), H115 (≠ E137), L120 (≠ F142), P122 (= P144), T123 (≠ D145)
- binding calcium ion: D43 (= D45), D45 (= D47)
Sites not aligning to the query:
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
30% identity, 41% coverage: 13:157/352 of query aligns to 34:176/285 of Q7CQ56
6eqoA Tri-functional propionyl-coa synthase of erythrobacter sp. Nap1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester (see paper)
26% identity, 67% coverage: 5:241/352 of query aligns to 859:1078/1804 of 6eqoA
Sites not aligning to the query:
- binding phosphomethylphosphonic acid adenylate ester: 456, 458, 535, 536, 537, 538, 558, 559, 560, 561, 562, 688, 714
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 1261, 1265, 1379, 1400, 1403, 1404, 1405, 1424, 1425, 1429, 1444, 1492, 1493, 1497, 1514, 1517, 1713, 1730, 1731, 1774
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
25% identity, 51% coverage: 13:192/352 of query aligns to 23:200/273 of Q5HH38
- R34 (≠ L24) binding in other chain
- SGGDQ 73:77 (≠ AGGDI 63:67) binding in other chain
- S149 (≠ F142) binding in other chain
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 50% coverage: 16:190/352 of query aligns to 16:185/257 of 6slbAAA
- active site: Q64 (≠ G65), F69 (≠ V70), L80 (≠ F86), N84 (≠ Y90), A108 (≠ G114), S111 (≠ R117), A130 (≠ P136), F131 (≠ E137), L136 (≠ F142), P138 (= P144), D139 (= D145)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R59), A62 (= A63), Q64 (≠ G65), D65 (= D66), L66 (≠ I67), Y76 (≠ S79), A108 (≠ G114), F131 (≠ E137), D139 (= D145)
Sites not aligning to the query:
Query Sequence
>WP_011384942.1 NCBI__GCF_000009985.1:WP_011384942.1
MDGETLISSGGRLGRIVLNRPKVLNALSARQYHDITACLVSWESDPDVAVILIEGEGERA
FCAGGDIRMVWDAARRGDSEFSHDVFRTEYRLNRRIHAYPKPYVSVLDGICMGGGARLSV
NGRFRVATERTRFAMPETGIGFFPDVGATHFLNRCPGDLGLYLGLTGKQLGPADCLWAGI
ATHFVPAARVDDLRAGLRVAAQSADPEDAVEDVLARFHQPVADGPLVRHMEALERCFAPR
RLDDMIEALRLDGGQWAWDTIWELSGRAPFSLAVTLRQLREGRGMGFDDAIRREFRLTWR
FLLGQDFLEGIRAQVVDKDRRPNWSPSSLAEVDELAVAACFQTLGDDELPLP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory