SitesBLAST
Comparing WP_011385134.1 NCBI__GCF_000009985.1:WP_011385134.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
72% identity, 96% coverage: 16:439/442 of query aligns to 4:427/427 of 2y27B
- binding adenosine-5'-triphosphate: K65 (= K77), S90 (= S102), S91 (= S103), G92 (= G104), T93 (= T105), T94 (= T106), F138 (= F150), A211 (= A223), E212 (= E224), P213 (= P225), D232 (= D244), I233 (= I245), Y234 (= Y246), G235 (= G247), L236 (= L248), S237 (= S249), D302 (= D314), I320 (= I332), R323 (= R335), K419 (= K431)
- binding magnesium ion: V200 (≠ K212), S202 (≠ C214), L204 (= L216), M226 (= M238), G227 (= G239), Q347 (≠ D359), L350 (= L362)
2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
72% identity, 96% coverage: 16:439/442 of query aligns to 4:425/426 of 2y4nA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (= Y145), F136 (= F150), G138 (= G152), G208 (= G222), A209 (= A223), E210 (= E224), P211 (= P225), I231 (= I245), Y232 (= Y246), G233 (= G247), L234 (= L248), S235 (= S249), P240 (= P254), D300 (= D314), R321 (= R335), K417 (= K431)
- binding magnesium ion: V198 (≠ K212), S200 (≠ C214), Q345 (≠ D359), L348 (= L362)
2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
68% identity, 96% coverage: 16:439/442 of query aligns to 6:432/432 of 2y4oB
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y145), F140 (= F150), G212 (= G222), A213 (= A223), E214 (= E224), P215 (= P225), I235 (= I245), G237 (= G247), L238 (= L248), S239 (= S249), P244 (= P254), D304 (= D314), R325 (= R335), I331 (= I341), N336 (= N346)
- binding magnesium ion: S204 (≠ C214), V228 (≠ M238)
2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
68% identity, 96% coverage: 16:439/442 of query aligns to 6:432/433 of 2y4oA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y145), F140 (= F150), A213 (= A223), E214 (= E224), P215 (= P225), I235 (= I245), G237 (= G247), L238 (= L248), S239 (= S249), P244 (= P254), D304 (= D314), R325 (= R335), I331 (= I341), N336 (= N346)
4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
43% identity, 96% coverage: 17:439/442 of query aligns to 9:433/435 of 4r1mA
- binding adenosine monophosphate: A215 (= A223), E216 (= E224), P217 (= P225), N236 (≠ D244), S237 (≠ I245), F238 (≠ Y246), G239 (= G247), M240 (≠ L248), T241 (≠ S249), D305 (= D314), R329 (= R335), I335 (= I341), N340 (= N346)
- binding zinc ion: C252 (= C260), H259 (= H268), C314 (vs. gap), C316 (≠ T322)
4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
42% identity, 96% coverage: 17:439/442 of query aligns to 9:431/433 of 4r1lA
- binding adenosine-5'-diphosphate: A215 (= A223), E216 (= E224), P217 (= P225), S237 (≠ I245), F238 (≠ Y246), G239 (= G247), M240 (≠ L248), T241 (≠ S249), D305 (= D314), R329 (= R335), N340 (= N346)
- binding adenosine monophosphate: A215 (= A223), E216 (= E224), P217 (= P225), S237 (≠ I245), F238 (≠ Y246), G239 (= G247), M240 (≠ L248), T241 (≠ S249), D305 (= D314), R329 (= R335), N340 (= N346)
- binding coenzyme a: S136 (= S144), A164 (≠ G172), G165 (= G173), N166 (≠ Q174), S167 (≠ T175), I185 (≠ T193), Y188 (= Y196), K337 (≠ R343), T408 (≠ S414)
- binding zinc ion: C252 (= C260), H259 (= H268), C314 (vs. gap), C316 (≠ T322)
6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
31% identity, 92% coverage: 34:439/442 of query aligns to 44:464/477 of 6he0A
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ G222), G242 (≠ A223), E243 (= E224), P244 (= P225), G267 (≠ Y246), S268 (≠ G247), M269 (≠ L248), A270 (≠ S249), D335 (= D314), I357 (= I332), N371 (= N346)
- binding adenosine monophosphate: G242 (≠ A223), E243 (= E224), P244 (= P225), C266 (≠ I245), G267 (≠ Y246), S268 (≠ G247), A270 (≠ S249), E271 (= E250), D335 (= D314), N371 (= N346)
- binding coenzyme a: Y166 (≠ F150), A188 (≠ G172), G189 (vs. gap), P191 (vs. gap), S194 (≠ T175), Y210 (≠ M191), G211 (≠ V192), T212 (= T193), Y215 (= Y196), H218 (≠ A199), R368 (= R343), G369 (= G344), M401 (≠ L376), V439 (≠ I413), R440 (≠ S414)
6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
30% identity, 92% coverage: 34:439/442 of query aligns to 44:461/474 of 6hdyA
- binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F150), S237 (≠ G222), G263 (≠ Y246), S264 (≠ G247), M265 (≠ L248), A266 (≠ S249), F271 (≠ P254)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F150), G164 (= G152), S237 (≠ G222), G238 (≠ A223), E239 (= E224), P240 (= P225), C262 (≠ I245), G263 (≠ Y246), S264 (≠ G247), A266 (≠ S249), F271 (≠ P254), D331 (= D314), I353 (= I332), R356 (= R335), K453 (= K431)
6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
30% identity, 92% coverage: 34:439/442 of query aligns to 44:461/474 of 6hdxA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F150), G164 (= G152), S237 (≠ G222), G238 (≠ A223), E239 (= E224), P240 (= P225), C262 (≠ I245), G263 (≠ Y246), S264 (≠ G247), A266 (≠ S249), F271 (≠ P254), D331 (= D314), I353 (= I332), R356 (= R335), K453 (= K431)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F150), G164 (= G152), S237 (≠ G222), G263 (≠ Y246), S264 (≠ G247), A266 (≠ S249), F271 (≠ P254)
6sixB Paak family amp-ligase with anp (see paper)
27% identity, 95% coverage: 7:425/442 of query aligns to 4:405/437 of 6sixB
- binding phosphoaminophosphonic acid-adenylate ester: S88 (= S102), S89 (= S103), A213 (= A223), E214 (= E224), P215 (= P225), E236 (≠ I245), Y237 (= Y246), G238 (= G247), S239 (≠ L248), T240 (≠ S249), E241 (= E250), D300 (= D314), V320 (≠ I332), R323 (= R335)
- binding magnesium ion: R79 (≠ M93), E80 (≠ D94), P121 (≠ R137), T150 (= T166)
- binding zinc ion: C249 (= C260), H255 (= H268), C309 (≠ G321), C311 (≠ A323)
6siyA Paak family amp-ligase with amp and substrate (see paper)
27% identity, 94% coverage: 10:425/442 of query aligns to 3:401/433 of 6siyA
- binding 3-hydroxyanthranilic acid: T125 (≠ Y145), P126 (≠ G146), T132 (≠ G152), L135 (≠ G155), R153 (≠ G173), N177 (≠ T193), A209 (= A223), E232 (≠ I245), G234 (= G247), S235 (≠ L248)
- binding adenosine monophosphate: S85 (= S103), A209 (= A223), E210 (= E224), P211 (= P225), E232 (≠ I245), Y233 (= Y246), G234 (= G247), S235 (≠ L248), T236 (≠ S249), D296 (= D314), V316 (≠ I332)
- binding magnesium ion: R75 (≠ M93), E76 (≠ D94), L78 (≠ V96), P117 (≠ R137), G144 (= G164), A145 (≠ C165), T146 (= T166)
6siwA Paak family amp-ligase with amp (see paper)
27% identity, 94% coverage: 10:425/442 of query aligns to 2:400/432 of 6siwA
- binding adenosine monophosphate: S84 (= S103), A208 (= A223), E209 (= E224), P210 (= P225), E231 (≠ I245), Y232 (= Y246), G233 (= G247), S234 (≠ L248), T235 (≠ S249), D295 (= D314), V315 (≠ I332)
- binding magnesium ion: E75 (≠ D94), L77 (≠ V96), S83 (= S102), P116 (≠ R137), G143 (= G164), T145 (= T166), E236 (= E250)
- binding zinc ion: C244 (= C260), H250 (= H268), C304 (≠ G321), C306 (≠ A323)
3cw8X 4-chlorobenzoyl-coa ligase/synthetase, bound to 4cba-adenylate (see paper)
24% identity, 58% coverage: 102:356/442 of query aligns to 161:421/501 of 3cw8X
- binding 5'-O-[(S)-{[(4-chlorophenyl)carbonyl]oxy}(hydroxy)phosphoryl]adenosine: H207 (≠ G148), V208 (≠ L149), V209 (≠ T151), G281 (≠ A223), A282 (≠ E224), T283 (≠ P225), I303 (= I245), Y304 (= Y246), G305 (= G247), T306 (≠ L248), T307 (≠ S249), M310 (≠ I252), N311 (≠ G253), M324 (≠ L269), D385 (= D314)
3cw9A 4-chlorobenzoyl-coa ligase/synthetase in the thioester-forming conformation, bound to 4-chlorophenacyl-coa (see paper)
24% identity, 58% coverage: 102:356/442 of query aligns to 161:421/503 of 3cw9A
- active site: T161 (≠ S102), R181 (≠ W122), H207 (≠ G148), T307 (≠ S249), E308 (= E250), I406 (= I341), N411 (= N346)
- binding 4-Chlorophenacyl-coenzyme A: M203 (≠ S144), P204 (≠ Y145), H207 (≠ G148), V208 (≠ L149), V209 (≠ T151), A280 (≠ G222), G305 (= G247), T306 (≠ L248), M310 (≠ I252), N311 (≠ G253), S407 (≠ I342), G408 (≠ R343), G409 (= G344), E410 (≠ V345)
- binding adenosine monophosphate: T161 (≠ S102), G281 (≠ A223), A282 (≠ E224), T283 (≠ P225), I303 (= I245), Y304 (= Y246), G305 (= G247), T306 (≠ L248), T307 (≠ S249), D385 (= D314), R400 (= R335), I406 (= I341), N411 (= N346)
Sites not aligning to the query:
Query Sequence
>WP_011385134.1 NCBI__GCF_000009985.1:WP_011385134.1
MSKTAHRRVPPAKDLLDPIEIASRDQITALQTERMKWSLTHAYNNVEHYRNKCREKGVHP
DDFKELKDLAKFPFTTKSDLRDTYPFGMFAVPMDEVVRVHASSGTTGKPTVVGYTQNDID
MWANVMARSIRAAGGRRSDKCHVSYGYGLFTGGLGAHYGAERLGCTVIPMSGGQTEKQIQ
LIMDFKPEIIMVTPSYMLAIADEMDRQGIDTKTCPLQVGIFGAEPWTGAMREEIEVRMGI
EAVDIYGLSEVIGPGVACECVETKDGLHLWEDHFYPEIIDPHTGEVLPDGSMGELVFTSL
TKEALPIIRYRTRDLTQLLPGTARSMRRMGKITGRSDDMLIIRGVNVFPTQIEELILKDY
RLAPHYQLEVTRDGHLDSVAVNVEVRADQPYDDSALEAASKDLQHHIKSYIGISTKVNVL
APNSIERSMGKARRVIDKRPKA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory