SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011385213.1 NCBI__GCF_000009985.1:WP_011385213.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P56459 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
42% identity, 97% coverage: 5:586/598 of query aligns to 2:575/577 of P56459

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P56459

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L81 (≠ T84) mutation to N: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by reducing enzyme's ability to misacylate tRNA(Asn).
L86 (= L89) mutation to M: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by reducing enzyme's ability to misacylate tRNA(Asn).

4rmfA Biochemical and structural characterization of mycobacterial aspartyl- tRNA synthetase asps, a promising tb drug target (see paper)
43% identity, 96% coverage: 5:581/598 of query aligns to 2:577/579 of 4rmfA

query
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4rmfA

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active site: R215 (= R221), E217 (= E223), R223 (= R229), Q224 (≠ S230), E481 (= E485), G484 (≠ S488), R536 (= R540)
binding 2,2-bis(hydroxymethyl)propane-1,3-diol: H447 (= H451), D474 (= D478), E481 (= E485)

Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
42% identity, 99% coverage: 3:594/598 of query aligns to 1:591/591 of Q51422

query
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Q51422

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M

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Y

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K

K

P

P

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D

L

L

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N

I

P

P

I

L

I

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I

L

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V

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D

V

V

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A

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G

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V

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L

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G

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L

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Y

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L

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D

D

G

G

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N

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D

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V

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M

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L

L

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D

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S

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V

I

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A

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F

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P

M

K

N

T

Q

Q

Q

S

A

A

Q

G

D

D

L

V

L

M

M

T

Q

Q

A

A

P

P

A

G

E

S

I

V

A

D

M

G

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K

R

A

L

L

R

R

E

E

L

L

H

H

I

I

K

R

V

L

D

R

L

-

P

E

K

Q

P

P

K

K

K

A

E

E

H31 (= H33) mutation to L: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 3.5-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA.
G82 (≠ E83) mutation to K: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 4.2-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA.

4wj4A Crystal structure of non-discriminating aspartyl-tRNA synthetase from pseudomonas aeruginosa complexed with tRNA(asn) and aspartic acid (see paper)
43% identity, 97% coverage: 5:586/598 of query aligns to 2:583/585 of 4wj4A

query
sites
4wj4A

R

R

S

S

H

H

T

Y

C

C

G

G

Q

Q

L

L

K

N

A

E

A

S

D

L

A

D

G

G

I

Q

Q

E

A

V

R

T

L

L

S

C

G

G

W

W

V

V

H

H

R
|
R

K

R

R
|
R

D
|
D

H
|
H

G
|
G

N
x
G

L
x
V

L

I

F
|
F

V

L

D

D

L

V

R

R

D

D

H

R

Y

E

G

G

I

L

T

A

Q
|
Q

C

V

V

V

I

F

D

D

V

P

S

D

-

R

S

A

P

E

V

T

F

F

A

A

A

K

L

A

D

D

K

R

A

V

R
|
R

P

S

E

E

S

F

V

V

I

V

T

K

V

I

T

T

G

G

K

K

V

V

V
x
R

K

L

R

R

S
x
P

A

E

E

G

T
x
A

I

R

N
|
N

P

P

R

N

L

M

P

A

T

S

G

G

E

S

I

I

E
|
E

L

V

Q

L

V

G

A

Y

E

E

V

L

E

E

I

V

Q

L

S

N

I

Q

A

A

D

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V
x
T

L

P

P
|
P

I

F

Q

P

V

L

A
x
D

G
x
E

D

Y

Q

S

E
x
D

Y

V

P

G

E

E

D
x
E

M

T

R

R

L

L

R

R

Y

Y

R

R

F

F

L

I

D

D

L

L

R

R

R

R

E

P

D

E

V

M

H

A

A

A

N

K

M

L

M

K

L

L

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R

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A

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A

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Y

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R

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N

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P

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L

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A

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S

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S

T

P

P

E

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G

G

A
|
A

R

R

D

D

Y

Y

L

L

V

V

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P

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R

R

I

T

H

Y

P

P

G

G

K

H

F

F

Y

F

A

A

L

L

P

P

Q

Q

A
x
S

P

P

Q
|
Q

Q

L

F

F

K

K

Q

Q

L

L

L

L

M

M

V

V

A

A

G

G

F

F

D

D

K

R

Y

Y

F

Y

Q

Q

I

I

A

A

P

K

C

C

F

F

R
|
R

D

D

E
|
E

A
x
D

G

L

R
|
R

A
|
A

D

D

R
|
R

S
x
Q

P

P

G

-

E

E

F

F

Y

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Q

Q

L

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D

D

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A

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V

M

L

V

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G

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F

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K

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K

-

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D

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V

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F

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F

F

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I

M

T

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Y

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A

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D

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A

M

M

L

R

K

R

Y

Y

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

N

I

P

P

I

L

I

E

I

L

A

V

D

D

V

V

T

A

E

D

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Q

F

L

R

K

G

E

S

V

G

E

F

F

G

K

L

V

F

F

A

S

K

G

L

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V

A

-

N

D

D

K

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G

K

A

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V

V

R

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A

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A

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D

D

K

D

L

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N

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D

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W

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A

V

R

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I

N
x
Y

G

G

A

A

G

K

G

G

L

L

G

A

Y

Y

I

I

Q

K

F

V

-

N

-

E

-

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-

A

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K

A

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A

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G

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L

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I

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K

K

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L

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P

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L

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N

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V

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I

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L

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D

A

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G

L

A

K

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D

D

G

G

D

D

A

I

V

V

F

F

F

F

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C

A

D

D

K

K

A

A

L

K

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I

A

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A

C

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D

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A

A

L

G

G

L

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L

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I

K

K

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H

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L

L

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L

L

L

L

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A

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W

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D

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F

P

P

M

M

Y

F

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I

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N

N

E

D

E

D

T

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G

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L

L

V

S

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x
A

F

L

S

-

H
|
H

N
x
H

P

P

F

F

S

T

M

S

P

P

Q

K

G

-

G

C

M

T

D

P

A

A

L

E

L

L

N

E

Q

A

D

N

P

P

L

G

T

A

I

A

N

L

A

S

Y

R

Q

A

Y

Y

D

D

I

M

V

V

C

L

N

N

G

G

V

T

E
|
E

L

L

S

G

S
x
G

G

G

A

S

I

I

R
|
R

N

I

H

H

R

D

P

K

D

S

I

M

M

Q

Y

Q

K

A

A

V

F

F

E

R

I

V

A

L

G

G

Y

I

S

D

A

E

A

A

H

E

V

Q

E

E

E

E

H

K

F

F

G

G

G

F

M

L

L

L

N

D

A

A

F

L

K

K

F

Y

G

G

A

A

P

P

P

P

H

H

G

G

G

G

S
x
L

A

A

P

F

G

G

V

L

D

D

R
|
R

I

L

V

V

M

M

L

L

L

M

A

T

D

G

Q

A

P

S

N

S

I

I

R
|
R

E

E

I

V

I

I

L

A

F

F

P

P

M

K

N
x
T

Q
|
Q

Q
x
S

A

A

Q

G

D

D

L

V

L

M

M

T

Q

Q

A

A

P

P

A

G

E

S

I

V

A

D

M

G

E

K

R

A

L

L

R

R

E

E

L

L

H

H

I

I

K

R

V

L

active site: R219 (= R221), E221 (= E223), R227 (= R229), Q228 (≠ S230), E482 (= E485), G485 (≠ S488), R537 (= R540)
binding aspartic acid: S195 (≠ A197), Q197 (= Q199), H450 (≠ N452), R489 (= R492), L531 (≠ S534)
binding : R26 (= R29), R28 (= R31), D29 (= D32), H30 (= H33), G31 (= G34), G32 (≠ N35), V33 (≠ L36), F35 (= F38), Q46 (= Q49), R64 (= R66), R76 (≠ V78), P79 (≠ S81), A82 (≠ T84), N84 (= N86), E93 (= E95), T107 (≠ V109), P109 (= P111), D113 (≠ A115), E114 (≠ G116), D117 (≠ E119), E121 (≠ D123), A175 (= A177), E221 (= E223), D222 (≠ A224), R224 (= R226), A225 (= A227), R227 (= R229), Y346 (≠ N351), A447 (≠ E448), H449 (= H451), H450 (≠ N452), R549 (= R552), T557 (≠ N560), Q558 (= Q561), S559 (≠ Q562)

4wj3M Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
43% identity, 97% coverage: 5:586/598 of query aligns to 2:583/589 of 4wj3M

query
sites
4wj3M

R

R

S

S

H

H

T

Y

C

C

G

G

Q

Q

L

L

K

N

A

E

A

S

D

L

A

D

G

G

I

Q

Q

E

A

V

R

T

L

L

S

C

G

G

W

W

V

V

H

H

R

R

K

R

R
|
R

D
|
D

H
|
H

G

G

N
x
G

L
x
V

L

I

F
|
F

V

L

D

D

L

V

R

R

D

D

H

R

Y

E

G

G

I

L

T

A

Q
|
Q

C

V

V

V

I

F

D

D

V

P

S

D

-

R

S

A

P

E

V

T

F

F

A

A

A

K

L

A

D

D

K

R

A

V

R
|
R

P

S

E

E

S

F

V

V

I

V

T

K

V

I

T

T

G

G

K

K

V

V

V
x
R

K

L

R
|
R

S

P

A

E

E

G

T
x
A

I

R

N
|
N

P

P

R

N

L

M

P

A

T

S

G

G

E

S

I

I

E
|
E

L

V

Q

L

V

G

A

Y

E

E

V

L

E

E

I

V

Q

L

S

N

I

Q

A

A

D

E

V
x
T

L

P

P

P

I

F

Q

P

V

L

A
x
D

G

E

D

Y

Q

S

E

D

Y
x
V

P

G

E

E

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L

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Y

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|
R

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|
E

A

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|
R

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x
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A

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M

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Y

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G

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D

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D

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V

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N

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G

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|
E

L

L

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x
G

G

G

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I

I

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K

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K

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|
R

I

L

V

V

M

M

L

L

L

M

A

T

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A

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S

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I

R

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V

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I

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F

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N

T

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Q

Q

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D

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M

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M

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K

R

A

L

L

R

R

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L

L

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H

I

I

K

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active site: R219 (= R221), E221 (= E223), R227 (= R229), Q228 (≠ S230), E482 (= E485), G485 (≠ S488), R537 (= R540)
binding : R28 (= R31), D29 (= D32), H30 (= H33), G32 (≠ N35), V33 (≠ L36), F35 (= F38), Q46 (= Q49), R64 (= R66), R76 (≠ V78), R78 (= R80), A82 (≠ T84), N84 (= N86), E93 (= E95), T107 (≠ V109), D113 (≠ A115), V118 (≠ Y120)

5w25A Crystal structure of aspartyl-tRNA synthetase from mycobacterium tuberculosis complexed with l-aspartic acid
43% identity, 97% coverage: 3:581/598 of query aligns to 2:582/583 of 5w25A

query
sites
5w25A

V

V

Y

L

R

R

S

S

H

H

T

A

C

A

G

G

Q

L

L

L

K

R

A

E

A

G

D

D

A

A

G

G

I

Q

Q

Q

A

V

R

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L

L

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A

G

G

W

W

V

V

H

A

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R

K

R

R

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D

D

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H

G

G

N

G

L

V

L

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F

F

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I

D

D

L

L

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R

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D

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A

Y

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G

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I

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Q

C

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V

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D

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D

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A

A

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M

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D

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|
E

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F

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A

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L

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|
Q

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K

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Q

L

L

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M

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V

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A

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G

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R

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Y

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Q

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C

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|
R

D

D

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|
E

A

D

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F

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A

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D

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|
R

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x
Q

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M

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V

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G

G

L

A

V

W

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H
|
H

N
x
H

P

A

F

F

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T

M

A

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G

P

G

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M

W

D

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A

D

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R

L

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N

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D

D

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T

L

G

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V

N

L

A

A

Y

D

Q

A

Y

Y

D

D

I

I

V

V

C

C

N

N

G

G

V

H

E
|
E

L

I

S

G

S
x
G

G

G

A

S

I

V

R
|
R

N

I

H

H

R

R

P

R

D

D

I

I

M

Q

Y

E

K

R

A

V

F

F

E

A

I

V

A

M

G

G

Y

L

S

D

A

K

A

A

H

E

V

A

E

E

E

E

H

K

F

F

G

G

G

F

M

L

L

L

N

E

A

A

F

F

K

M

F

F

G

G

A

A

P

P

P

P

H

H

G

G

G

G

S

I

A

A

P

F

G

G

V

W

D

D

R
|
R

I

T

V

T

M

A

L

L

L

L

A

A

D

G

Q

M

P

D

N

S

I

I

R

R

E

E

I

V

I

I

L

A

F

F

P

P

M

K

N

T

Q

G

Q

G

A

G

Q

V

D

D

L

P

L

L

M

T

Q

D

A

A

P

P

A

A

E

P

I

I

A

T

M

A

E

Q

R

Q

L

R

R

K

E

E

active site: R220 (= R221), E222 (= E223), R228 (= R229), Q229 (≠ S230), E486 (= E485), G489 (≠ S488), R541 (= R540)
binding aspartic acid: E174 (= E175), Q198 (= Q199), R220 (= R221), H452 (= H451), H453 (≠ N452), G489 (≠ S488), R493 (= R492)
binding lysine: D159 (≠ G160), R211 (≠ K212)

4o2dA Crystal structure of aspartyl-tRNA synthetase from mycobacterium smegmatis with bound aspartic acid (see paper)
43% identity, 97% coverage: 3:584/598 of query aligns to 1:579/580 of 4o2dA

query
sites
4o2dA

V

V

Y

L

R

R

S

T

H

H

T

A

C

A

G

G

Q

S

L

L

K

R

A

P

A

A

D

D

A

A

G

G

I

Q

Q

T

A

V

R

T

L

L

S

A

G

G

W

W

V

V

H

A

R

R

K

R

R

R

D

D

H

H

G

G

N

G

L

V

L

I

F

F

V

I

D

D

L

L

R

R

D

D

H

A

Y

S

G

G

I

V

T

S

Q

Q

C

V

V

V

I

F

D

R

V

-

S

E

S

G

P

D

V

V

F

L

A

A

A

A

L

A

D

H

K

R

A

L

R

R

P

A

E

E

S

F

V

C

I

V

T

A

V

V

T

T

G

G

K

V

V

V

V

E

K

V

R

R

S

P

A

E

E

G

T

N

I

E

N

N

P

P

R

E

L

I

P

P

T

T

G

G

E

Q

I

I

E

E

L

V

Q

N

V

A

A

T

E

E

V

L

E

T

I

V

Q

L

S

G

I

E

A

S

D

A

V

P

L

L

P

P

I

F

Q

Q

V

L

A

-

G

-

D

D

Q

E

E

Q

Y

A

P

G

E

E

D

E

M

A

R

R

L

L

R

K

Y

Y

R

R

F

Y

L

L

D

D

L

L

R

R

R

R

E

E

D

G

V

P

H

G

A

N

N

A

M

L

M

R

L

L

R

R

S

S

R

K

V

V

I

N

A

A

Y

A

L

A

R

R

Q

S

A

V

M

L

I

A

G

E

Q

H

G

D

F

F

T

V

E

E

F

I

Q

E

T

T

P

P

I

T

L

L

T

T

A

R

S

S

S

T

P

P

E
|
E

G

G

A

A

R

R

D

D

Y

F

L

L

V

V

P

P

S

A

R

R

I

L

H

Q

P

P

G

G

K

S

F

F

Y

Y

A

A

L

L

P

P

Q

Q

A
x
S

P

P

Q
|
Q

Q

L

F

F

K

K

Q

Q

L

L

L

L

M

M

V

V

A

A

G

G

F

M

D

E

K

R

Y

Y

F

Y

Q

Q

I

I

A

A

P

R

C

C

F

Y

R
|
R

D

D

E
|
E

A

-

G

-

R

D

A

F

D

D

R
|
R

S
x
Q

P

P

G

-

E

E

F

F

Y

T

Q
|
Q

L

L

D

D

F

M

E

E

M

M

S

S

Y

F

V

V

T

E

Q

A

D

D

D

D

V

V

F

I

A

A

A

I

I

S

E

E

P

Q

V

V

L

L

E

K

G

A

V

V

F

W

K

A

E

T

F

I

G

G

K

Y

G

D

R

-

A

-

V

-

T

L

P

P

A

L

P

P

F

L

P

P

R

R

I

I

T

S

Y

Y

A

E

D

E

S

A

M

M

L

R

K

R

Y

F

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

N

F

P

G

I

I

I

E

I

L

A

V

D

E

V

C

T

T

E

E

P

Y

F

F

R

K

G

D

S

T

G

T

F

F

G

R

L

V

F

F

A

-

K

-

L

-

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-

D

-

K

Q

G

A

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Y

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Q

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D

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G

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L

L

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Y

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A

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D

D

G

G

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-

L

K

G

G

G

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P

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A

A

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K

N

N

L

L

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S

P

D

A

A

R

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V

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A

G

I

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K

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A

A

A

H

A

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N

G

L

A

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N

D

P

G

G

D

D

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C

V

I

F

F

F

F

A

A

C

A

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K

P

A

A

L

K

P

G

A

A

A

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K

A

F

L

A

L

G

G

L

A

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T

R

R

T

I

K

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I

I

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A

N

K

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L

L

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D

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W

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F

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M

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D

E

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-

A

-

T

-

A

-

A

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G

-

D

-

V

-

A

-

V

-

G

T

S

G

G

L

A

V

W

E

T

F

A

S

M

H
|
H

N
x
H

P

A

F

F

S

T

M

A

P

P

Q

K

G

P

G

D

M

S

D

V

A

D

L

T

L

F

N

D

Q

S

D

D

P

P

L

G

T

N

I

A

N

L

A

S

Y

D

Q

A

Y

Y

D

D

I

I

V

V

C

C

N

N

G

G

V

N

E
|
E

L

I

S

G

S
x
G

G

G

A

S

I

I

R
|
R

N

I

H

H

R

R

P

R

D

D

I

I

M

Q

Y

E

K

R

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F

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A

M

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G

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A

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A

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Q

E

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F

F

G

G

G

F

M

L

L

L

N

D

A

A

F

F

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S

F

Y

G

G

A

A

P

P

P

P

H

H

G

G

G

G

S
x
I

A
|
A

P

F

G

G

V

W

D

D

R
|
R

I

I

V

T

M

A

L

L

L

L

A

A

D

G

Q

V

P

D

N

S

I

I

R

R

E

E

I

V

I

I

L

A

F

F

P

P

M

K

N

S

Q

G

Q

G

A

G

Q

V

D

D

L

P

L

L

M

T

Q

D

A

A

P

P

A

A

E

P

I

I

A

T

M

P

E

Q

R

Q

L

R

R

K

E

E

L

S

H

G

I

I

active site: R216 (= R221), E218 (= E223), R222 (= R229), Q223 (≠ S230), E480 (= E485), G483 (≠ S488), R535 (= R540)
binding aspartic acid: E170 (= E175), S192 (≠ A197), Q194 (= Q199), Q228 (= Q236), H446 (= H451), H447 (≠ N452), G483 (≠ S488), R487 (= R492), I529 (≠ S534), A530 (= A535)

1c0aA Crystal structure of the e. Coli aspartyl-tRNA synthetase : trnaasp : aspartyl-adenylate complex (see paper)
40% identity, 97% coverage: 5:586/598 of query aligns to 2:583/585 of 1c0aA

query
sites
1c0aA

R

R

S

T

H

E

T

Y

C

C

G

G

Q

Q

L

L

K

R

A

L

A

S

D

H

A

V

G

G

I

Q

Q

Q

A

V

R

T

L

L

S

C

G

G

W

W

V

V

H

N

R
|
R

K

R

R
|
R

D
|
D

H
x
L

G
|
G

N
x
S

L
|
L

L

I

F
|
F

V

I

D

D

L

M

R

R

D

D

H

R

Y

E

G

G

I

I

T

V

Q
|
Q

C

V

V
x
F

I

F

D
|
D

V
x
P

S

D

-

R

S

A

P

D

V

A

F

L

A

K

A

L

L

A

D

S

K

E

A

L

R
|
R

P

N

E

E

S

F

V

C

I

I

T

Q

V

V

T

T

G

G

K

T

V

V

V
x
R

K

A

R
|
R

S

D

A

E

E

K

T
x
N

I

I

N
|
N

P

R

R

D

L
x
M

P

A

T

T

G

G

E

E

I

I

E
|
E

L

V

Q

L

V

A

A

S

E

S

V

L

E

T

I

I

Q

I

S

N

I

R

A

A

D

D

V

V

L

L

P
|
P

I

L

Q
x
D

V

-

A

-

G

S

D
x
N

Q
x
H

E

V

Y
x
N

P
x
T

E

E

D
x
E

M

A

R

R

L

L

R

K

Y

Y

R

R

F

Y

L

L

D

D

L

L

R

R

R

R

E

P

D

E

V

M

H

A

A

Q

N

R

M

L

M

K

L

T

R

R

S

A

R

K

V

I

I

T

A

S

Y

L

L

V

R

R

Q

R

A

F

M

M

I

D

G

D

Q

H

G

G

F

F

T

L

E

D

F

I

Q

E

T

T

P

P

I

M

L

L

T

T

A

K

S

A

S
x
T

P
|
P

E
|
E

G
|
G

A
|
A

R

R

D

D

Y

Y

L

L

V

V

P

P

S

S

R

R

I

V

H

H

P

K

G

G

K

K

F

F

Y

Y

A

A

L

L

P

P

Q

Q

A
x
S

P

P

Q
|
Q

Q

L

F

F

K
|
K

Q

Q

L

L

L

L

M

M

V

M

A

S

G

G

F

F

D

D

K

R

Y

Y

F

Y

Q

Q

I

I

A

V

P

K

C

C

F

F

R
|
R

D

D

E
|
E

A
x
D

G

L

R
|
R

A
|
A

D

D

R
|
R

S
x
Q

P

P

G

-

E

E

F
|
F

Y

T

Q
|
Q

L

I

D

D

F

V

E

E

M

T

S

S

Y

F

V

M

T

T

Q

A

D

P

D

Q

V

V

F

R

A

E

A

V

I

M

E

E

P

A

V

L

L

V

E

R

G

H

V

L

F

W

K

L

E

E

F

V

G

-

K

-

G

-

R

K

A

G

V

V

T

D

P

L

A

G

P

D

F

F

P

P

R

V

I

M

T

T

Y

F

A

A

D

E

S

A

M

E

L

R

K

R

Y

Y

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

N

N

P

P

I

M

I

E

I

L

A

T

D

D

V

V

T

A

E

D

P

L

F

L

R

K

G

S

S

V

G

E

F
|
F

G

A

L
x
V

F

F

A

A

K

G

L

P

V

A

-

N

D

D

K

P

G

K

A

G

V

R

V

V

R

A

A

A

I

L

P

R

A

V

P

P

G

G

A

G

A

A

G

S

Q

L

P

T

R

R

S

K

W

Q

F

I

D

D

K

E

L

Y

N

G

D

N

W

F

A

V

R

K

E
x
I

N

Y

G

G

A

A

G
x
K

G
|
G

L

L

G
x
A

Y

Y

I

I

Q

K

F

V

-

N

-

E

-

R

-

A

-

K

A

G

A

L

D

E

G

G

P

I

K

N

G

S

P

P

I

V

A

A

K

K

N

F

L

L

E

N

P

A

A

E

R

I

V

I

E

E

A

D

I

I

K

L

A

D

A

R

A

T

N

A

L

A

K