SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011385236.1 NCBI__GCF_000009985.1:WP_011385236.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
61% identity, 98% coverage: 1:190/193 of query aligns to 3:192/195 of P48636

query
sites
P48636

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E74 (= E72) binding
M89 (= M87) mutation to A: Almost complete loss of activity.
R92 (= R90) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 90:91) binding
109:115 (vs. 107:113, 86% identical) binding
T112 (= T110) mutation to A: Almost complete loss of activity.
E114 (= E112) mutation to A: Almost complete loss of activity.
E115 (= E113) mutation to A: Almost complete loss of activity.
E118 (= E116) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
62% identity, 94% coverage: 1:181/193 of query aligns to 2:182/182 of 5zbkA

query
sites
5zbkA

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binding adenosine monophosphate: A11 (≠ S10), E73 (= E72), M88 (= M87), R91 (= R90), K92 (= K91), G108 (= G107), G110 (= G109), T111 (= T110)

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
50% identity, 94% coverage: 1:181/193 of query aligns to 2:181/181 of 5zblD

query
sites
5zblD

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binding adenosine monophosphate: S11 (= S10), M47 (= M46), R90 (= R90), K91 (= K91), G107 (= G107), G109 (= G109), T110 (= T110), E112 (= E112), E113 (= E113)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
49% identity, 94% coverage: 1:181/193 of query aligns to 35:215/242 of Q5ZC82

query
sites
Q5ZC82

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G73 (= G39) mutation to D: In LOG-4; decreased activity.

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
39% identity, 94% coverage: 3:183/193 of query aligns to 4:207/217 of 5ajuA

query
sites
5ajuA

R

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binding 5-O-phosphono-beta-D-ribofuranose: S11 (= S10), R108 (= R90), G131 (= G107), Y132 (≠ I108), G133 (= G109), T134 (= T110), E137 (= E113)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 85% coverage: 4:167/193 of query aligns to 13:176/187 of O05306

query
sites
O05306

V

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D

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N

M54 (= M46) mutation to A: Large decrease in catalytic activity.
K74 (≠ E66) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E72) mutation to A: Large decrease in catalytic activity.
R98 (= R90) mutation to A: Loss of catalytic activity.
T118 (= T110) mutation to A: Loss of catalytic activity.
DE 120:121 (≠ EE 112:113) mutation to AA: Loss of catalytic activity.

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
45% identity, 85% coverage: 4:167/193 of query aligns to 9:172/183 of 7w2iA

query
sites
7w2iA

V

V

C

A

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C

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H

D

P

H

D

D

L

A

T

D

E

E

L

L

R

I

V

V

V

T

A

D

S

T

M

M

H

W

E

E

R

R

K

K

A

Q

A

I

M

M

A

E

D

D

L

R

A

S

D

D

A

A

F

F

I

I

A

V

L

L

P

P

G

G

I

V

G

G

T

T

L

L

E

D

E

E

L

L

F

F

E
x
D

I

A

W

W

T

T

W
x
D

G

G

Q

Y

L

L

G

G

L

T

H

H

A

D

K

K

P

P

L

I

G

V

F

M

L

V

D

D

V

P

A

W

G

G

Y

H

F

F

E

D

R

G

L

L

H

R

A

A

F

W

L

L

D

N

H

G

M

L

A

L

A

D

E

T

G

G

F

Y

V

V

K

S

A

P

R

T

H

A

R

M

E

E

-

R

M

L

V

V

A

V

V

V

H

D

N

N

binding magnesium ion: D120 (≠ E116), D124 (≠ W120)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
43% identity, 87% coverage: 4:171/193 of query aligns to 4:172/177 of 3sbxA

query
sites
3sbxA

V

V

C

A

V

V

F

Y

C

C

G

A

S

A

N

-

S

A

G

P

A

T

N

H

P

P

A

E

Y

L

A

L

K

E

A

L

A

A

A

G

Q

A

L

V

G

G

R

A

L

A

L

I

A

A

E

A

R

R

G

G

Q

W

V

T

L

L

V

V

Y

W

G

G

N

H

V

V

G

S

L

A

M

M

G

G

V

A

V

V

A

S

D

S

A

A

L

R

A

A

A

H

G

G

Q

W

V

T

I

V

G

G

V

V

I

I

P

P

E

K

S

M

M

L

L

V

K

H

W

R

E
|
E

V

L

G

A

H

D

P

H

D

D

L

A

T

D

E

E

L

L

R

V

V

V

V

T

A

E

S

T

M
|
M

H

W

E

E

R
|
R

K
|
K

A

Q

A

V

M

M

A

E

D

D

L

R

A

A

D

N

A

A

F

F

I

I

A

T

L

L

P

P

G

G

G
|
G

I

V

G
|
G

T
|
T

L

L

E
x
D

E
|
E

L

L

F

L

E

D

I

V

W

W

T

T

W

E

G

G

Q

Y

L

L

G

G

L

M

H

H

A

D

K

K

P

S

L

I

G

V

F

V

L

L

D

D

V

P

A

W

G

G

Y

H

F

F

E

D

R

G

L

L

H

R

A

A

F

W

L

L

D

S

H

E

M

L

A

A

A

D

E

T

G

G

F

Y

V

V

K

S

-

R

-

T

A

A

R

M

H

E

R

R

E

L

M

I

V

V

A

V

V

D

H

N

N

L

D

D

P

D

A

A

I

L

binding adenosine monophosphate: E71 (= E72), M86 (= M87), R89 (= R90), K90 (= K91), G106 (= G107), G108 (= G109), T109 (= T110), D111 (≠ E112), E112 (= E113)

6y01DDD p450 cytochrome, putative (see paper)
35% identity, 52% coverage: 14:114/193 of query aligns to 16:114/166 of 6y01DDD

query
sites
6y01DDD

A

A

N

T

P

P

A

T

Y

D

A

L

K

K

A

N

A

A

A

Y

Q

Q

L

L

G

G

R

Q

L

L

L

I

A

A

E

L

R

E

G

G

Q

W

V

V

L

L

V

L

Y

T

G

G

G

R

N

N

V

V

G

G

L

V

M

M

G

E

V

H

V

A

A

S

D

Q

A

G

A

A

L

K

A

K

A

A

G

E

G

G

Q

L

V

T

I

I

G

G

V

I

I

L

P

P

E

S

S

-

M

-

L

-

K

K

W

N

E

T

V

H

G

N

H

V

P

S

D

D

L

A

T

V

E

D

L

I

R

A

V

I

V

V

A

T

S

G

M

L

H

G

E

N

R

A

K

R

A

N

M

I

A

N

D

V

L

L

-

S

A

S

D

D

A

V

F

V

I

I

A

A

L

C

P

G

G

I

G
|
G

I

L

G

G

T
|
T

L

L

E

S

E

E

L

V

binding chloride ion: G107 (= G107), T110 (= T110)

Query Sequence

>WP_011385236.1 NCBI__GCF_000009985.1:WP_011385236.1
MKRVCVFCGSNSGANPAYAKAAAQLGRLLAERGQVLVYGGGNVGLMGVVADAALAAGGQV
IGVIPESMLKWEVGHPDLTELRVVASMHERKAAMADLADAFIALPGGIGTLEELFEIWTW
GQLGLHAKPLGFLDVAGYFERLHAFLDHMAAEGFVKARHREMVAVHNDPAILLALLDSYR
PPETIRVIDRETA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory