SitesBLAST

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
29% identity, 43% coverage: 57:169/263 of query aligns to 23:152/191 of G3XD01

query
sites
G3XD01

V

V

S

W

H

H

V

F

G

V

K

H

I

I

L

C

T

A

G

G

I

A

E

R

I

I

H

G

P

A

A

G

A

V

Q

S

L

L

G

G

P

Q

R

N

F

V

F

F

I

V

D

-

H

-

G

G

T

N

G

K

V

V

I

I

G

G

E

D

T

R

A

C

V

K

I

I

G

Q

A

N

D

N

V

V

T

S

I

V

Y

Y

H

D

G

N

V

V

T

T

L

L

G

E

-

G

-

V

-

F

-

C

-

G

-

P

-

S

-

M

-

V

-

F

T

T

S

N

L

V

H

Y

K

N

G

P

K

R

-

S

-

L

-

I

-

E

-

R

-

K

-

D

-

Q

-

Y

R

R

H

N

P

T

T

L

L

V

E

K

D

K

G

G

V

A

I

T

V

L

G

G

S

A

G

N

A

C

Q

T

V

I

L

V

G

C

P

G

I

V

T

T

V

I

G

G

K

E

G

Y

A

A

R

F

I

V

G

G

A

A

N

G

A

A

V

V

V

I

L

N

T
x
K

D

N

V

V

P

P

P

S

G

Y

V

A

T

L

M

M

V

V

G

G

I

V

P

P

A

A

R

R

M

Q

V

I

K136 (≠ T153) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 41% coverage: 74:180/263 of query aligns to 77:193/203 of P07464

query
sites
P07464

Q

H

L

I

G

G

P

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

T

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

V

T

I

I

G

G

A

D

D

N

V

V

T

L

I

I

Y

A

H

P

G

N

V

V

T

T

L

L

G

S

-

V

-

T

G

G

T
x
H

S

P

L

V

H

H

K

H

G

E

K

L

R

R

H

K

P

N

-

G

-

E

-

M

-

Y

-

S

-

F

-

P

-

I

T

T

L

I

E

G

D

N

G

N

V

V

I

W

V

I

G

G

S
|
S

G

H

A

V

Q

V

V

I

L

N

G

P

P

G

I

V

T

T

V

I

G

G

K

D

G

N

A

S

R

V

I

I

G

G

A
|
A

N

G

A

S

V

I

V

V

L
x
T

T
x
K

D

D

V

I

P

P

G

N

V

V

T

V

M

A

V

A

G

G

I

V

P

P

A

C

R
|
R

M

V

V

I

M
x
R

R

E

R

I

Q

N

D

D

A

R

D

D

F

K

C

H

A

Y

Y

Y

N85 (≠ D82) binding in other chain; binding in other chain
D93 (≠ G90) binding
H115 (≠ T110) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S129) binding in other chain
A160 (= A147) binding in other chain
TK 165:166 (≠ LT 152:153) binding
R180 (= R167) binding
R183 (≠ M170) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
29% identity, 41% coverage: 74:180/263 of query aligns to 76:192/200 of 1krrA

query
sites
1krrA

Q

H

L

I

G

G

P

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

T

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

V

T

I

I

G

G

A

D

D

N

V

V

T

L

I
|
I

Y
x
A

H
x
P

G

N

V

V

T

T

L

L

G

S

-

V

-
x
T

G

G

T
x
H

S

P

L

V

H

H

K

H

G

E

K

L

R

R

H

K

P

N

-

G

-

E

-

M

-

Y

-

S

-

F

-

P

-

I

T

T

L

I

E

G

D

N

G

N

V

V

I

W

V

I

G
|
G

S
|
S

G

H

A

V

Q

V

V

I

L
x
N

G

P

P

G

I

V

T

T

V

I

G

G

K

D

G

N

A

S

R

V

I

I

G
|
G

A
|
A

N

G

A

S

V

I

V

V

L

T

T

K

D

D

V

I

P

P

G

N

V

V

T

V

M

A

V
x
A

G

G

I

V

P
|
P

A

C

R

R

M

V

V

I

M
x
R

R

E

R

I

Q

N

D

D

A

R

D

D

F

K

C

H

A

Y

Y

Y

binding acetyl coenzyme *a: N84 (≠ D82), I103 (= I101), A104 (≠ Y102), P105 (≠ H103), T112 (vs. gap), H114 (≠ T110), G140 (= G128), S141 (= S129), N146 (≠ L134), G158 (= G146), A159 (= A147), A174 (≠ V162), P177 (= P165), R182 (≠ M170)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
29% identity, 41% coverage: 74:180/263 of query aligns to 76:192/201 of 1krvA

query
sites
1krvA

Q

H

L

I

G

G

P

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

T

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

V

T

I

I

G

G

A

D

D

N

V

V

T

L

I

I

Y
x
A

H

P

G

N

V

V

T

T

L

L

G
x
S

-

V

-
x
T

G

G

T

H

S

P

L

V

H

H

K

H

G

E

K

L

R

R

H

K

P

N

-

G

-

E

-
x
M

-

Y

-

S

-

F

-

P

-

I

T

T

L

I

E

G

D

N

G

N

V

V

I
x
W

V

I

G
|
G

S
|
S

G

H

A

V

Q

V

V

I

L
x
N

G

P

P

G

I

V

T

T

V

I

G

G

K

D

G

N

A

S

R

V

I

I

G

G

A
|
A

N

G

A

S

V

I

V

V

L

T

T

K

D

D

V

I

P

P

G

N

V

V

T

V

M

A

V

A

G

G

I

V

P
|
P

A

C

R
|
R

M

V

V

I

M
x
R

R

E

R

I

Q

N

D

D

A

R

D

D

F

K

C

H

A

Y

Y

Y

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F80), N84 (≠ D82), V90 (= V88), D92 (≠ G90), M126 (vs. gap)
binding coenzyme a: A104 (≠ Y102), S110 (≠ G108), T112 (vs. gap), W138 (≠ I126), G140 (= G128), S141 (= S129), N146 (≠ L134), A159 (= A147), P177 (= P165), R179 (= R167), R182 (≠ M170)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

Query Sequence

>WP_011385407.1 NCBI__GCF_000009985.1:WP_011385407.1
MIFKSLQEDIASIRKRDPAAHSWLEVLLCYPGLHALMFHRLSNWCWNRGLRVLGRFVSHV
GKILTGIEIHPAAQLGPRFFIDHGTGVVIGETAVIGADVTIYHGVTLGGTSLHKGKRHPT
LEDGVIVGSGAQVLGPITVGKGARIGANAVVLTDVPPGVTMVGIPARMVMRRQDADFCAY
GLPVEDLPDPVVRAIDSVRSQVATLMERIKELETELHGHPTQACARLSQPEGAEPGDKTK
QVEAVTMPAGGTTGRTLERETIS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory