SitesBLAST

Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
36% identity, 74% coverage: 42:219/242 of query aligns to 2:225/234 of Q7WTT9

query
sites
Q7WTT9

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C12 (= C52) binding
C15 (= C55) binding
C18 (= C58) binding
C22 (= C62) binding
C57 (= C97) binding
C60 (= C100) binding
C65 (= C105) binding
C69 (= C109) binding
C89 (= C129) binding
C92 (= C132) binding
C95 (= C135) binding
C99 (= C139) binding
C164 (= C156) binding
C167 (= C159) binding
C179 (= C171) binding
C183 (= C175) binding

2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
42% identity, 53% coverage: 91:219/242 of query aligns to 130:258/337 of 2ivfB

query
sites
2ivfB

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binding fe3-s4 cluster: C148 (= C109), T150 (≠ V111), I153 (≠ T114), C169 (= C129), K170 (≠ V130), G171 (= G131), H172 (≠ C132), R173 (≠ A133), H174 (≠ Y134), C175 (= C135), S193 (≠ A153)
binding protoporphyrin ix containing fe: T150 (≠ V111), K170 (≠ V130), H172 (≠ C132)
binding iron/sulfur cluster: M135 (≠ L96), C136 (= C97), N137 (= N98), H138 (= H99), C139 (= C100), P142 (= P103), C144 (= C105), V162 (= V122), C179 (= C139), A183 (= A143), I184 (≠ R144), K195 (= K155), C196 (= C156), I197 (≠ T157), L198 (≠ F158), C199 (= C159), N209 (≠ P169), C211 (= C171), C215 (= C175), V219 (≠ A179), R220 (= R180)

Sites not aligning to the query:

binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41

5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
42% identity, 53% coverage: 91:219/242 of query aligns to 127:255/329 of 5ch7B

query
sites
5ch7B

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binding fe3-s4 cluster: C145 (= C109), I150 (≠ T114), C166 (= C129), K167 (≠ V130), G168 (= G131), A169 (≠ C132), Q170 (≠ A133), A171 (≠ Y134), C172 (= C135), A190 (= A153)
binding iron/sulfur cluster: P130 (= P94), C133 (= C97), N134 (= N98), C136 (= C100), P139 (= P103), C141 (= C105), C176 (= C139), C193 (= C156), G195 (≠ F158), C196 (= C159), C208 (= C171), C212 (= C175), V213 (= V176), G214 (= G177)

Sites not aligning to the query:

binding iron/sulfur cluster: 17, 18, 19, 20, 21, 23, 27, 31, 43, 45

P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
39% identity, 60% coverage: 42:187/242 of query aligns to 4:157/205 of P18776

query
sites
P18776

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C102 (= C132) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1kqfB Formate dehydrogenase n from e. Coli (see paper)
32% identity, 80% coverage: 44:237/242 of query aligns to 30:253/289 of 1kqfB

query
sites
1kqfB

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binding protoporphyrin ix containing fe: Y137 (= Y134), W252 (= W236)
binding iron/sulfur cluster: K31 (≠ M45), C38 (= C52), I39 (= I53), G40 (= G54), C41 (= C55), K42 (≠ Q56), C44 (= C58), C48 (= C62), N52 (= N66), T77 (= T75), M79 (≠ V77), C99 (= C97), M100 (≠ N98), H101 (= H99), C102 (= C100), P105 (= P103), C107 (= C105), C111 (= C109), P112 (= P110), I117 (≠ T114), V125 (= V122), C132 (= C129), I133 (≠ V130), G134 (= G131), C135 (= C132), G136 (≠ A133), Y137 (= Y134), C138 (= C135), C142 (= C139), I146 (≠ A143), P147 (≠ R144), V156 (≠ A153), K158 (= K155), C159 (= C156), L161 (≠ F158), C162 (= C159), P172 (= P169), C174 (= C171), C178 (= C175), P179 (≠ V176), I183 (≠ R180)

P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
32% identity, 80% coverage: 44:237/242 of query aligns to 31:254/294 of P0AAJ3

query
sites
P0AAJ3

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P

P

E

E

Q

G

G

V

T

G

A

G

P

T

N

H

V

V

Y

M

Y

Y

I

V

-

L

-

H

-

A

G

D

L

Q

D

P

E

E

R

L

F

Y

A

H

G

G

-

L

-

P

-

K

-

D

-

P

R

K

V

I

D

D

G

T

T

S

P

V

T

S

L

L

W

W

R

K

C39 (= C52) binding
C42 (= C55) binding
C45 (= C58) binding
C49 (= C62) binding
C100 (= C97) binding
C103 (= C100) binding
C108 (= C105) binding
C112 (= C109) binding
C133 (= C129) binding
C136 (= C132) binding
C139 (= C135) binding
C143 (= C139) binding
C160 (= C156) binding
C163 (= C159) binding
C175 (= C171) binding
C179 (= C175) binding

3egwB The crystal structure of the narghi mutant narh - c16a
43% identity, 42% coverage: 93:194/242 of query aligns to 180:282/509 of 3egwB

query
sites
3egwB

L

L

P
|
P

R

R

L

L

C
|
C

N
x
E

H
|
H

C
|
C

S

L

D

N

P
|
P

P

A

C
|
C

V

V

G

A

V

T

C
|
C

P

P

V
x
S

G

G

A

A

T
x
I

F

Y

Q

K

-

R

Q

E

K

E

D

D

G

G

A

I

V

V

M

L

V

I

D

D

S

Q

D

D

R

K

C
|
C

V
x
R

G
|
G

C
x
W

A
x
R

Y

M

C
|
C

V

I

Q

T

A

G

C
|
C

P

P

Y

Y

D

K

A

K

R
x
I

F

Y

I

F

N

N

H

W

E

K

T

S

N

G

T

K

A
x
S

D

E

K
|
K

C
|
C

T

I

F
|
F

C
|
C

G

Y

H

P

R

R

V

I

A

E

A

A

G

G

L

Q

L

P

P
x
T

A

V

C
|
C

V

S

E

E

T

T

C
|
C

V

V

G
|
G

G

R

A

I

R
|
R

V

Y

F

L

G

G

D

V

L

L

N

L

E

Y

T

D

E

A

G

D

A

A

V

I

R

E

R

R

binding fe3-s4 cluster: P181 (= P94), C196 (= C109), S198 (≠ V111), I201 (≠ T114), C217 (= C129), R218 (≠ V130), G219 (= G131), W220 (≠ C132), R221 (≠ A133), C223 (= C135), S241 (≠ A153), C263 (= C175), G265 (= G177), R268 (= R180)
binding protoporphyrin ix containing fe: W220 (≠ C132), R221 (≠ A133)
binding iron/sulfur cluster: C184 (= C97), E185 (≠ N98), H186 (= H99), C187 (= C100), P190 (= P103), C192 (= C105), C227 (= C139), I232 (≠ R144), K243 (= K155), C244 (= C156), F246 (= F158), C247 (= C159), T257 (≠ P169), C259 (= C171)

Sites not aligning to the query:

binding fe3-s4 cluster: 17, 18, 19, 20, 22
binding protoporphyrin ix containing fe: 88, 89
binding iron/sulfur cluster: 26, 30, 41, 42

P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
43% identity, 42% coverage: 93:194/242 of query aligns to 180:282/512 of P11349

query
sites
P11349

L

L

P

P

R

R

L

L

C
|
C

N

E

H

H

C
|
C

S

L

D

N

P

P

P

A

C
|
C

V

V

G

A

V

T

C
|
C

P

P

V

S

G

G

A

A

T

I

F

Y

Q

K

-

R

Q

E

K

E

D

D

G

G

A

I

V

V

M

L

V

I

D

D

S

Q

D

D

R

K

C
|
C

V

R

G

G

C

W

A

R

Y

M

C
|
C

V

I

Q

T

A

G

C
|
C

P

P

Y

Y

D

K

A

K

R

I

F

Y

I

F

N

N

H

W

E

K

T

S

N

G

T

K

A

S

D

E

K

K

C
|
C

T

I

F

F

C
|
C

G

Y

H

P

R

R

V

I

A

E

A

A

G

G

L

Q

L

P

P

T

A

V

C
|
C

V

S

E

E

T

T

C
|
C

V

V

G

G

G

R

A

I

R

R

V

Y

F

L

G

G

D

V

L

L

N

L

E

Y

T

D

E

A

G

D

A

A

V

I

R

E

R

R

C184 (= C97) binding
C187 (= C100) binding
C192 (= C105) binding
C196 (= C109) binding
C217 (= C129) binding
C223 (= C135) binding
C227 (= C139) binding
C244 (= C156) binding
C247 (= C159) binding
C259 (= C171) binding
C263 (= C175) binding

Sites not aligning to the query:

16 binding
19 binding
22 binding
26 binding

4v4cB Pyrogallol hydroxytransferase small subunit (see paper)
31% identity, 75% coverage: 41:221/242 of query aligns to 2:191/274 of 4v4cB

query
sites
4v4cB

R

E

R

Q

W

Y

A

Y

M

M

V

V

V

I

D

D

L

V

G

A

K

K

C
|
C

I
x
Q

G
x
D

C
|
C

Q
x
N

A
x
N

C
|
C

T

F

I

M

G

G

C
|
C

I

M

M

D

E

E

N

H

R

E

V

L

P

-

A

-

D

N

S

E

F

W

R

P

T

G

F

Y

V

T

S

A

T

S

Y

M

E

Q

-

R

-

G

-

H

-

R

-

W

-

M

V

N

T

I

E

E

G

R

D

R

K

E

V

R

G

G

T

T

Y

Y

-

P

-

R

-

N

-

D

-
x
I

-
x
N

V

Y

L

R

P

P

R

T

L

P

C
|
C

N
x
M

H
|
H

C
|
C

S

E

D

N

P

A

P

P

C
|
C

V

V

G

A

V

K

C

G

P

N

V

-

G

G

A

A

T

V

F

Y

Q

Q

Q

R

K

E

D

D

G

G

A

I

V
|
V

M

L

V

I

D

D

S

P

D

E

R

K

C

A

V

K

G

G

C

K

A

K

Y

E

C

L

V

L

Q

D

A

T

C
|
C

P

P

Y

Y

D

G

A
x
V

R

M

F

Y

I

W

N

N

H

E

E

E

T

E

N

N

T

V

A

A

D

Q

K

K

C
|
C

T

T

F
x
M

C
|
C

G

A

H

H

R

L

V

L

-

D

-

E

-

S

A

W

A

A

G

P

L

K

L

M

P
|
P

A

R

C
|
C

V

A

E

H

T

N

C
|
C

V

-

G

-

G

G

A
x
S

R

F

V
|
V

F
x
Y

G

E

D

F

L

L

N

K

E

T

T

T

E

P

G

E

A

A

V

M

R

A

R

K

L

K

L

V

D

E

A

E

N

E

K

G

G

-

R

-

T

L

K

E

V

V

L

I

K

K

P

P

E

E

Q

L

G

G

T

T

A

K

P

P

N

R

V

V

Y

Y

I

K

G

N

L

L

binding calcium ion: I61 (vs. gap), N62 (vs. gap)
binding iron/sulfur cluster: C13 (= C52), Q14 (≠ I53), D15 (≠ G54), C16 (= C55), N17 (≠ Q56), N18 (≠ A57), C19 (= C58), C23 (= C62), C68 (= C97), M69 (≠ N98), H70 (= H99), C71 (= C100), C76 (= C105), V92 (= V122), C109 (= C139), V113 (≠ A143), C126 (= C156), M128 (≠ F158), C129 (= C159), P143 (= P169), C145 (= C171), C149 (= C175), S151 (≠ A179), V153 (= V181), Y154 (≠ F182)

6f0kB Alternative complex iii (see paper)
29% identity, 62% coverage: 42:192/242 of query aligns to 723:923/961 of 6f0kB

query
sites
6f0kB

R

Q

W

W

A

A

M

M

V

V

V

I

D

D

L

L

G

N

K

A

C
|
C

I
x
T

G
|
G

C
|
C

Q
x
N

A
|
A

C
|
C

T

I

I

V

G

A

C
|
C

I

D

M

S

E

E

N

N

R

N

V

I

P

P

A

M

-

V

-

G

-

K

-

N

-

E

-

V

-

G

-

R

-

G

-

R

D

E

S

M

F

H

R
x
W

T

L

F

R

V
x
I

S

D

T

R

Y

Y

E

F

V

V

T

S

E

D

-

E

-

A

-

H

G

A

D

D

K

D

V

P

G

Q

T

I

Y

V

V

V

L

Q

P

P

R

V

L

P

C
|
C

N
x
M

H
|
H

C
|
C

S

E

D

N

P

A

P
|
P

C
|
C

V

E

G

S

V

V

C
|
C

P

P

V
|
V

G

A

A

A

T

T

F

V

Q

H

Q

S

K

P

D

D

G

G

A

L

V
x
N

M

E

V
x
M

D

V

S

Y

D
x
N

R
|
R

C
|
C

V
x
I

G
|
G

C

T

A

R

Y

Y

C
|
C

V

S

Q

N

A

N

C
|
C

P

P

Y

Y

D

K

A

V

R

R

F

R

I

F

N

N

H

W

E

F

T

N

-

W

-

V

-

K

-

T

-

L

-

P

-

I

-

Q

-

V

-

Q

-

M

-

A

-

Q

-

N

-

P

-

D

-

V

-

T

-

V

-

R

-

F

-

R

N

G

T

V

A
x
M

D

E

K

K

C
|
C

T

T

F
x
Y

C
|
C

G

V

H

Q

R

R

V

I

A

R

A

E

G

A

-

Q

-
x
R

-

Q

-

A

-
x
N

-

I

-

E

-

K

-

R

-

P

-

L

-

R

-

D

-

G

-

E

L

V

L

K

P

T

A
|
A

C
|
C

V

Q

E

Q

T

A

C
|
C

V

P

G

A

G

E

A

A

R

I

V

T

F

F

G

G

D

D

L

L

N

N

E

D

T

P

E

N

G

N

A

A

V

V

binding fe3-s4 cluster: C803 (= C109), V805 (= V111), M818 (≠ V124), C823 (= C129), I824 (≠ V130), G825 (= G131), C829 (= C135), M869 (≠ A153)
binding heme c: N821 (≠ D127), R822 (= R128), R884 (vs. gap), N887 (vs. gap)
binding iron/sulfur cluster: C733 (= C52), T734 (≠ I53), G735 (= G54), C736 (= C55), N737 (≠ Q56), A738 (= A57), C739 (= C58), C743 (= C62), W765 (≠ R74), I768 (≠ V77), C791 (= C97), M792 (≠ N98), H793 (= H99), C794 (= C100), P798 (= P104), C799 (= C105), N816 (≠ V122), C833 (= C139), C872 (= C156), Y874 (≠ F158), C875 (= C159), A901 (= A170), C902 (= C171), C906 (= C175)

Sites not aligning to the query:

binding heme c: 719

6btmB Structure of alternative complex iii from flavobacterium johnsoniae (wild type) (see paper)
27% identity, 72% coverage: 45:218/242 of query aligns to 686:942/948 of 6btmB

query
sites
6btmB

M

L

V

S

V

I

D

D

L

L

G

N

K

A

C

C

I

T

G

G

C

C

Q

G

A

A

C

C

T

V

I

I

G

A

C

C

I

H

M

A

E

E

N

N

R

N

V

V

P

P

A

V

-

V

-

G

-

K

-

A

-

E

-

V

-

R

-

S

-

R

-

D

-

M

-

H

-

W

-

L

-

R

-

I

D

D

S

R

F

Y

R

Y

T

S

F

S

V

E

S

S

T

T

Y

F

E

E

-

G

-

D

-

N

-

E

-

R

-

K

-

E

-

G

-

I

-

A

-

G

-

L

-

S

-

L

-

S

-

T

-

F

-

N

-

E

-

M

V

E

T

K

E

P

G

G

D

D

K

N

V

P

G

Q

T

V

Y

A

V

F

L

Q

P

P

R

V

L

M

C
|
C

N
x
Q

H

H

C
|
C

S

N

D

H

P

A

P

P

C
|
C

V

E

G

T

V

V

C
|
C

P

P

V

V

G

A

A

A

T

T

F

S

Q

H

Q

G

K

R

D

Q

G

G

A

Q

V
x
N

M

H

V

M

D

A

S

Y

D

N

R

R

C
|
C

V
|
V

G

G

C
x
T

A

R

Y

Y

C
|
C

V

A

Q

N

A

N

C
|
C

P

P

Y

Y

D

K

A

V

R
|
R

-

R

-

F

-

N

-

W

-

F

-

L

-

Y

-

N

-

K

-

N

-

S

-

E

-

F

-

D

-

Y

-

H

-

M

-

N

-

D

-

L

-

G

-

R

-

M

F

V

I

L

N

N

H

P

E

D

T

V

N

N

T

V

-

R

-

S

-

R

-

G

-

V

A
x
M

D

E

K

K

C

C

T

S

F

F

C

C

G

I

H

Q

R

S

V

T

A

Q

A

A

G

V

L

I

L

L

P

E

A

A

C

K

V

R

E

Q

T

G

C

R

V

V

-

V

-

G

-

K

-

D

-

E

-

F

-

N

-

A

-

C

-

A

-

C

-

S

-

A

-

C

-

S

G

S

G

G

A

A

R

M

V

V

F

F

G

G

D

D

L

V

N

N

E

D

T

K

E

E

G

S

A

E

V

V

R

A

R

K

L

L

L

-

D

-

A

A

N

E

K

S

G

E

R

R

T

M

K

Y

V

H

L

L

K

L

P

E

E

H

Q

V

G

G

T

T

A

K

P

P

N

N

V

V

Y

F

Y

Y

binding fe3-s4 cluster: C789 (= C109), C809 (= C129), V810 (= V130), T812 (≠ C132), C815 (= C135), M862 (≠ A153)
binding iron/sulfur cluster: C777 (= C97), Q778 (≠ N98), C780 (= C100), C785 (= C105), N802 (≠ V122), C819 (= C139), R824 (= R144)

Sites not aligning to the query:

binding (2R)-3-{[(2R)-2-(decanoylamino)-3-hydroxypropyl]sulfanyl}propane-1,2-diyl ditetradecanoate: 1, 2

6lodB Cryo-em structure of the air-oxidized photosynthetic alternative complex iii from roseiflexus castenholzii (see paper)
27% identity, 74% coverage: 42:219/242 of query aligns to 682:917/929 of 6lodB

query
sites
6lodB

R

K

W

W

A

G

M

M

V

S

V

I

D

D

L

L

G

N

K

V

C
|
C

I
x
N

G
x
S

C
|
C

Q
x
N

A
|
A

C
|
C

T

V

I

V

G

A

C
|
C

I

Q

M

S

E

E

N
|
N

R

N

V

I

P

P

A

V

-

V

-

G

-

K

-

D

-

E

-

V

-

W

-

L

-

G

-

R

D

E

S

M

F

H

R
x
W

T

I

F

R

V
x
I

S

D

T

Q

Y

Y

E

Y

V

V

T

G

E

D

G

E

D

H

K

T

V

P

G

N

T

V

Y

Y

V

N

L

M

P

V

R

M

L
|
L

C
|
C

N
x
Q

H
x
Q

C
|
C

S

E

D

H

P

A

P
|
P

C
|
C

V

E

G

I

V
|
V

C
|
C

P
|
P

V

V

G

A

A

A

T

T

F

V

Q

H

Q

D

K

A

D

E

G

G

A

L

V
x
N

M

N

V

M

D

V

S

Y

D
x
N

R
|
R

C
|
C

V
|
V

G
|
G

C
x
T

A

K

Y

Y

C
|
C

V

S

Q

N

A

N

C
|
C

P

P

Y
|
Y

D

K

A
x
V

R
|
R

-

R

-

F

-

N

F

F

I

L

N

Q

H

Y

E

Q

T

D

-

V

-

P

-

Y

-

R

-

S

-

P

-

I

-

D

-

A

-

S

-

T

-

E

-

N

-

D

-

S

-

I

-

P

-

V

-

L

-

K

-

M

-

R

-

N

-

P

-

D

-

V

-

T

-

V

-

R

-

A

-

R

N

G

T

V

A
x
M

D

E

K
|
K

C
|
C

T

T

F
|
F

C
|
C

-

V

-

Q

-

R

-

I

-

N

-

E

-

A

-
x
R

-
x
I

-

Q

-

A

-
x
R

-

T

-

E

G

N

H

R

R

R

V

I

A

A

A

D

G

G

-

E

L

I

L

M

P
x
T

A

A

C
|
C

V

Q

E

Q

T

V

C
|
C

V

P

G

T

G

Q

A

A

R
x
I

V

V

F

F

G

G

D

D

L

L

N

N

E

D

T

P

E

Q

G

A

A

R

V

V

R

V

R

D

L

L

L

K

D

E

A

Q

N

P

K

L

G

K

R

Y

T

T

K

S

V

L

L

D

K

K

P

-

E

-

Q

L

G

N

T

T

A

K

P

P

N

R

V

V

Y

S

Y

Y

I

L

binding fe3-s4 cluster: V758 (= V108), C759 (= C109), P760 (= P110), C779 (= C129), V780 (= V130), G781 (= G131), T782 (≠ C132), C785 (= C135), M838 (≠ A153)
binding heme c: N777 (≠ D127), R778 (= R128), R852 (vs. gap), I853 (vs. gap), R856 (vs. gap)
binding iron/sulfur cluster: C692 (= C52), N693 (≠ I53), S694 (≠ G54), C695 (= C55), N696 (≠ Q56), A697 (= A57), C698 (= C58), C702 (= C62), N706 (= N66), W724 (≠ R74), I727 (≠ V77), L746 (= L96), C747 (= C97), Q748 (≠ N98), Q749 (≠ H99), C750 (= C100), P754 (= P104), C755 (= C105), N772 (≠ V122), C789 (= C139), Y791 (= Y141), V793 (≠ A143), R794 (= R144), K840 (= K155), C841 (= C156), F843 (= F158), C844 (= C159), T869 (≠ P169), C871 (= C171), C875 (= C175), I880 (≠ R180)

7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
33% identity, 53% coverage: 91:219/242 of query aligns to 168:305/409 of 7b04A

query
sites
7b04A

Y

F

V

Y

L

L

P
x
Q

R

R

L
x
I

C
|
C

N
|
N

H
|
H

C
|
C

S

T

D

Y

P
|
P

P

G

C
|
C

V

L

G

A

V

A

C
|
C

P

P

V
x
R

G

K

A

A

T
x
I

F

Y

Q

K

Q

R

K

K

-

E

D

D

G

G

A

I

V

V

M

L

V

I

D

D

S

Q

D

K

R

R

C
|
C

V
x
R

G
|
G

C
x
Y

A
x
R

Y
x
K

C
|
C

V

V

Q

E

A

Q

C
|
C

P

P

Y

Y

D

K

A

K

R
x
P

F

M

I

Y

N

R

H

G

E

L

T

T

N

R

T

V

A
x
S

D

E

K
|
K

C
|
C

T

I

F
x
A

C
|
C

G

Y

H

P

R

R

V

I

A

E

A

G

-

R

-

D

-

S

-

L

-

T

-

D

-

G

-

R

G

P

L

M

L

E

P
x
T

A
x
R

C
|
C

V

M

E

S

T

A

C
|
C

V
|
V

G

G

G

Q

A
x
I

R
|
R

V

L

F

Q

G

G

D

F

L

L

N

D

E

D

T

N

E

P

G

K

A

N

V

P

R

I

R

T

L

W

L

L

D

I

A

R

N

H

K

Q

G

K

R

I

T

A

K

L

V

P

L

L

K

Y

P

P

E

Q

Q

F

G

G

T

T

A

E

P

P

N

N

V

I

Y

Y

I

I

binding fe3-s4 cluster: C186 (= C109), I191 (≠ T114), C207 (= C129), R208 (≠ V130), G209 (= G131), Y210 (≠ C132), R211 (≠ A133), K212 (≠ Y134), C213 (= C135), S231 (≠ A153)
binding protoporphyrin ix containing fe: R188 (≠ V111), R208 (≠ V130), Y210 (≠ C132)
binding iron/sulfur cluster: Q171 (≠ P94), I173 (≠ L96), C174 (= C97), N175 (= N98), H176 (= H99), C177 (= C100), P180 (= P103), C182 (= C105), C217 (= C139), P222 (≠ R144), K233 (= K155), C234 (= C156), A236 (≠ F158), C237 (= C159), T255 (≠ P169), R256 (≠ A170), C257 (= C171), C261 (= C175), V262 (= V176), I265 (≠ A179), R266 (= R180)

Sites not aligning to the query:

binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62

8cm6D W-formate dehydrogenase c872a from desulfovibrio vulgaris - with formamide (see paper)
32% identity, 64% coverage: 45:200/242 of query aligns to 4:182/214 of 8cm6D

query
sites
8cm6D

M
x
F

V

F

V

V

D

D

L

L

G

S

K

R

C
|
C

I
x
T

G
x
A

C
|
C

Q
x
R

A

G

C
|
C

T

Q

I

I

G

A

C
|
C

I

K

M

Q

E

W

N
x
K

R

N

V

L

P

P

A

A

D

E

-

E

-

T

-

R

-

N

-

T

-

G

-

S

-

H

-

Q

-

N

-

P

-

D

-

L

-

S

-

Y

-

V

S

T

F

L

R
x
K

T

T

F

V

V

R

S

F

T

T

Y

E

E

K

V

S

T
x
R

E

K

G

G

D
x
P

K

G

V

I

G

D

T

W

Y

L

V

F

L

F

P

P

R

E

L

Q

C
|
C

N
x
R

H

H

C
|
C

S

V

D

E

P
|
P

C
|
C

V

K

G

G

V

Q

C

A

P

D

V

V

-

D

-

L

-

E

G

G

A

A

T

V

F

V

Q

K

-

D

Q

E

K

T

D

T

G

G

A

A

V
|
V

M

L

-

F

V

T

D

E

S

L

D

T

R

A

C

K

V

V

G

D

C

G

A

E

Y

S

C

V

V

R

Q

S

A

A

C
|
C

P

P

Y

Y

D

D

A
x
I

R
x
P

F

R

I

I

N

D

H

P

E

V

T

T

N

K

T

R

A

L

D

S

K
|
K

C
|
C

T
x
D

F
x
M

C
|
C

G

N

H

D

R

R

V

V

A

Q

A

N

G

G

L

L

P

P

A

A

C
|
C

V

V

E

K

T

T

C
|
C

V
x
P

G
x
T

G

G

A
x
T

R
x
M

V

N

F

F

G

G

D

D

L

E

N

Q

E

E

T

M

E

L

G

A

-

L

A

A

V

E

R

K

R

R

L

L

L

A

D

E

A

V

N

K

K

K

binding formamide: R59 (≠ T83), P62 (≠ D86)
binding iron/sulfur cluster: F4 (≠ M45), C11 (= C52), T12 (≠ I53), A13 (≠ G54), C14 (= C55), R15 (≠ Q56), C17 (= C58), C21 (= C62), K25 (≠ N66), K50 (≠ R74), C73 (= C97), R74 (≠ N98), C76 (= C100), P79 (= P103), P80 (= P104), C81 (= C105), V102 (= V122), C120 (= C139), I124 (≠ A143), P125 (≠ R144), K136 (= K155), C137 (= C156), D138 (≠ T157), M139 (≠ F158), C140 (= C159), C152 (= C171), C156 (= C175), P157 (≠ V176), T158 (≠ G177), T160 (≠ A179), M161 (≠ R180)

8bqgB W-formate dehydrogenase from desulfovibrio vulgaris - soaking with formate 1 min (see paper)
32% identity, 64% coverage: 45:200/242 of query aligns to 4:182/214 of 8bqgB

query
sites
8bqgB

M
x
F

V

F

V

V

D

D

L

L

G

S

K

R

C
|
C

I
x
T

G
x
A

C
|
C

Q
x
R

A

G

C
|
C

T

Q

I

I

G

A

C
|
C

I

K

M

Q

E

W

N
x
K

R

N

V

L

P

P

A

A

D

E

-

E

-

T

-

R

-

N

-

T

-

G

-

S

-

H

-

Q

-

N

-

P

-

D

-

L

-

S

-

Y

-

V

S

T

F

L

R
x
K

T

T

F

V

V

R

S

F

T

T

Y

E

E

K

V

S

T

R

E

K

G

G

D

P

K

G

V

I

G

D

T

W

Y

L

V

F

L

F

P

P

R

E

L

Q

C
|
C

N
x
R

H

H

C
|
C

S

V

D

E

P

P

P
|
P

C
|
C

V

K

G

G

V

Q

C

A

P

D

V

V

-

D

-

L

-

E

G

G

A

A

T

V

F

V

Q

K

-

D

Q

E

K

T

D

T

G

G

A

A

V
|
V

M

L

-

F

V

T

D

E

S

L

D

T

R

A

C

K

V

V

G

D

C

G

A

E

Y

S

C

V

V

R

Q

S

A

A

C
|
C

P

P

Y

Y

D

D

A
x
I

R
x
P

F

R

I

I

N

D

H

P

E

V

T

T

N

K

T

R

A

L

D

S

K
|
K

C
|
C

T

D

F
x
M

C
|
C

G

N

H

D

R

R

V

V

A

Q

A

N

G

G

L

L

P

P

A

A

C
|
C

V

V

E

K

T

T

C
|
C

V
x
P

G
x
T

G

G

A
x
T

R
x
M

V

N

F

F

G

G

D

D

L

E

N

Q

E

E

T

M

E

L

G

A

-

L

A

A

V

E

R

K

R

R

L

L

L

A

D

E

A

V

N

K

K

K

binding iron/sulfur cluster: F4 (≠ M45), C11 (= C52), T12 (≠ I53), A13 (≠ G54), C14 (= C55), R15 (≠ Q56), C17 (= C58), C21 (= C62), K25 (≠ N66), K50 (≠ R74), C73 (= C97), R74 (≠ N98), C76 (= C100), P80 (= P104), C81 (= C105), V102 (= V122), C120 (= C139), I124 (≠ A143), P125 (≠ R144), K136 (= K155), C137 (= C156), M139 (≠ F158), C140 (= C159), C152 (= C171), C156 (= C175), P157 (≠ V176), T158 (≠ G177), T160 (≠ A179), M161 (≠ R180)

Q8GC87 Formate dehydrogenase subunit beta; FDH subunit beta; Formate dehydrogenase small subunit from Megalodesulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) (Desulfovibrio gigas) (see paper)
29% identity, 60% coverage: 45:190/242 of query aligns to 5:172/215 of Q8GC87

query
sites
Q8GC87

M

F

V

F

V

V

D

D

L

T

G

T

K

R

C
|
C

I

T

G

A

C
|
C

Q

R

A

G

C
|
C

T

Q

I

V

G

A

C
|
C

I

K

M

Q

E

W

N

H

R

G

V

N

P

P

A

A

D

T

-

P

-

T

-

E

-

N

-

T

-

G

-

F

-

H

-

Q

-

N

-

P

-

D

-

F

S

N

F

F

R

H

T

T

F

Y

-

K

-

L

V

V

S

R

T

M

Y

H

E

E

V

Q

T

E

E

I

G

D

D

G

K

R

V

I

G

D

T

W

Y

L

V

F

L

F

P

P

R

D

L

Q

C
|
C

N

R

H

H

C
|
C

S

I

D

A

P

P

C
|
C

V

-

G

-

V

-

C

-

P

-

V

-

G

K

A

A

T

T

F

A

Q

D

Q

M

K

E

D

D

G

E

A

S

V

A

M

I

V

I

D

H

S

D

D

D

R

-

C

A

V

T

G

G

C

C

A

V

Y

L

-

F

-

T

-

P

-

K

-

T

-

K

-

D

-

L

-

E

-

D

-

Y

-

E

-

S

C

V

V

I

Q

S

A

A

C
|
C

P

P

Y

Y

D

D

A

V

R

P

F

R

I

K

N

V

H

A

E

E

T

S

N

N

T

Q

A

M

D

A

K

K

C
|
C

T

D

F

M

C
|
C

G

I

H

D

R

R

V

I

A

T

A

N

G

G

L

L

L

R

P

P

A

A

C
|
C

V

V

E

T

T

S

C
|
C

V

P

G

T

G

G

A

A

R

M

V

N

F

F

G

G

D

D

L

L

N

S

E

E

T

M

E

E

G

A

C12 (= C52) binding
C15 (= C55) binding
C18 (= C58) binding
C22 (= C62) binding
C73 (= C97) binding
C76 (= C100) binding
C81 (= C105) binding
C121 (= C139) binding
C138 (= C156) binding
C141 (= C159) binding
C153 (= C171) binding
C157 (= C175) binding

1h0hB Tungsten containing formate dehydrogenase from desulfovibrio gigas (see paper)
29% identity, 60% coverage: 45:190/242 of query aligns to 4:171/214 of 1h0hB

query
sites
1h0hB

M
x
F

V

F

V

V

D

D

L

T

G

T

K

R

C
|
C

I
x
T

G
x
A

C
|
C

Q
x
R

A

G

C
|
C

T

Q

I

V

G

A

C
|
C

I

K

M

Q

E

W

N
x
H

R

G

V

N

P

P

A

A

D

T

-

P

-

T

-

E

-

N

-

T

-

G

-

F

-

H

-

Q

-

N

-

P

-

D

-

F

S

N

F

F

R

H

T

T

F

Y

-
x
K

-

L

V

V

S

R

T

M

Y

H

E

E

V

Q

T

E

E

I

G

D

D

G

K

R

V

I

G

D

T

W

Y

L

V

F

L

F

P

P

R

D

L
x
Q

C
|
C

N
x
R

H

H

C
|
C

S

I

D

A

P

P

P
|
P

C
|
C

V

-

G

-

V

-

C

-

P

-

V

-

G

K

A

A

T

T

F

A

Q

D

Q

M

K

E

D

D

G

E

A

S

V

A

M

I

V

I

D

H

S

D

D

D

R

-

C

A

V

T

G

G

C

C

A
x
V

Y

L

-

F

-

T

-

P

-

K

-

T

-

K

-

D

-

L

-

E

-

D

-

Y

-

E

-

S

C

V

V

I

Q

S

A

A

C
|
C

P

P

Y

Y

D

D

A

V

R
x
P

F

R

I

K

N

V

H

A

E

E

T

S

N

N

T

Q

A

M

D

A

K
|
K

C
|
C

T
x
D

F
x
M

C
|
C

G

I

H

D

R

R

V

I

A

T

A

N

G

G

L

L

L

R

P

P

A

A

C
|
C

V

V

E

T

T

S

C
|
C

V
x
P

G

T

G

G

A
|
A

R

M

V

N

F

F

G

G

D

D

L

L

N

S

E

E

T

M

E

E

G

A

binding iron/sulfur cluster: F4 (≠ M45), C11 (= C52), T12 (≠ I53), A13 (≠ G54), C14 (= C55), R15 (≠ Q56), C17 (= C58), C21 (= C62), H25 (≠ N66), K50 (vs. gap), Q71 (≠ L96), C72 (= C97), R73 (≠ N98), C75 (= C100), P79 (= P104), C80 (= C105), V101 (≠ A133), C120 (= C139), P125 (≠ R144), K136 (= K155), C137 (= C156), D138 (≠ T157), M139 (≠ F158), C140 (= C159), C152 (= C171), C156 (= C175), P157 (≠ V176), A160 (= A179)

Query Sequence

>WP_011385651.1 NCBI__GCF_000009985.1:WP_011385651.1
MTHSRRDLLKGMGRITAGAVAVAAIPAAAEAAASPGGDPARRWAMVVDLGKCIGCQACTI
GCIMENRVPADSFRTFVSTYEVTEGDKVGTYVLPRLCNHCSDPPCVGVCPVGATFQQKDG
AVMVDSDRCVGCAYCVQACPYDARFINHETNTADKCTFCGHRVAAGLLPACVETCVGGAR
VFGDLNETEGAVRRLLDANKGRTKVLKPEQGTAPNVYYIGLDERFAGRVDGTPTLWRPSS
HV

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory