SitesBLAST

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
40% identity, 69% coverage: 82:286/298 of query aligns to 3:207/211 of Q58989

query
sites
Q58989

R

K

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K

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K

L

L

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A

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D
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D

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S

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D11 (= D90) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D92) active site, Proton donor; binding
E20 (= E99) binding
R56 (= R135) binding
SG 99:100 (= SG 178:179) binding
K144 (= K223) binding
D167 (= D246) binding
N170 (= N249) binding

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
40% identity, 69% coverage: 82:286/298 of query aligns to 2:206/210 of 1f5sA

query
sites
1f5sA

R

K

K

K

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K

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L

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D

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x
F

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D

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T

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L

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N

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N

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active site: D10 (= D90), F11 (≠ M91), D12 (= D92), G99 (= G179), K143 (= K223), D170 (= D250)
binding magnesium ion: D10 (= D90), D12 (= D92), D166 (= D246)
binding phosphate ion: D10 (= D90), F11 (≠ M91), D12 (= D92), S98 (= S178), G99 (= G179), K143 (= K223)

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
40% identity, 69% coverage: 82:286/298 of query aligns to 1:205/209 of 1l7nA

query
sites
1l7nA

R

K

K

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active site: D9 (= D90), F10 (≠ M91), D11 (= D92), G98 (= G179), K142 (= K223), D169 (= D250)
binding aluminum fluoride: D9 (= D90), F10 (≠ M91), D11 (= D92), S97 (= S178), K142 (= K223)
binding tetrafluoroaluminate ion: D9 (= D90), F10 (≠ M91), D11 (= D92), S97 (= S178), G98 (= G179), K142 (= K223), N168 (= N249)
binding magnesium ion: D9 (= D90), D11 (= D92), D165 (= D246)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
40% identity, 68% coverage: 83:286/298 of query aligns to 1:204/208 of 1l7pA

query
sites
1l7pA

R

K

K

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active site: N8 (≠ D90), F9 (≠ M91), D10 (= D92), G97 (= G179), K141 (= K223), D168 (= D250)
binding phosphoserine: N8 (≠ D90), F9 (≠ M91), D10 (= D92), E17 (= E99), M40 (= M122), F46 (= F128), R53 (= R135), S96 (= S178), G97 (= G179), K141 (= K223)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
39% identity, 68% coverage: 83:286/298 of query aligns to 1:196/200 of 1l7oA

query
sites
1l7oA

R

K

K

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R

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A

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N

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C

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L

binding zinc ion: C186 (≠ R276), E188 (≠ D278)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
39% identity, 72% coverage: 82:296/298 of query aligns to 177:391/409 of O53289

query
sites
O53289

R

R

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M

E

E

L

Q

A

T

V

V

S

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

D

D

M

M

L

L

R

G

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

A

E

E

V

A

A

R

D

V

A

R

S

V

L

D

S

H

H

G

P

D

Y

L

L

V

D

T

T

L

V

L

L

Y

F

A

L

Q

L

G

G

Y

V

A

T

D

R

D

G

E

E

M

I

D185 (= D90) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M91) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D92) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S93) mutation to A: No effect on enzymatic activity.
S273 (= S178) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K223) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D246) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D250) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
36% identity, 90% coverage: 30:296/298 of query aligns to 117:389/396 of 8a21A

query
sites
8a21A

A

A

L

L

G

G

A

V

E

N

V

I

G

D

H

L

A

I

R

R

W

G

L

V

S

S

P

D

E

Y

H

P

A

V

C

I

D

G

L

L

D

E

F

L

S

R

E

V

L

S

D

V

P

P

R

P

Q

G

A

A

D

D

Q

E

V

A

A

L

-

R

-

T

-

A

-

L

A

N

R

R

I

V

L

S

E

S

G

E

W

E

N

H

V

V

D

D

V

V

V

A

A

V

Q

E

-

D

-

Y

K

T

A

L

E

E

G

R

R

R

R

A

K

K

M

R

L

L

L

I

V

V

A

F

D
|
D

M

V

D
|
D

S

S

T

T

M

L

V

V

I

Q

G

G

E
|
E

T
x
V

L
x
I

D

E

E

M

L

L

A

A

D

A

F

K

A

A

G

G

L

A

K

E

D

G

H

Q

I

V

A

A

R

A

I

I

T
|
T

A

D

R

A

A

A

M
|
M

N

R

G

G

E

E

I

L

G

D

F
|
F

E

A

A

Q

A

S

L

L

R

Q

E

Q

R
|
R

V

V

G

A

L

T

L

L

K

A

D

G

L

L

P

P

E

A

E

T

C

V

L

I

Q

D

K

E

T

V

W

A

D

G

R

Q

I

L

E

E

F

L

T

M

P

P

G

G

A

A

H

R

K

T

L

T

V

L

R

R

T

T

M

L

V

R

K

R

H

L

G

G

A

Y

H

A

A

C

V

G

L

V

V

V

S

S

G

G

G
|
G

F

F

K

R

F

R

F

I

T

I

S

E

K

P

V

L

R

A

D

E

A

E

C

L

G

M

F

L

H

D

R

Y

D

V

I

A

A

A

N

N

E

E

L

L

I

E

V

I

E

V

N

D

G

G

R

T

L

L

T

T

G

G

Q

R

V

V

G

V

D

G

T

P

I

I

G

D

R

R

E

A

A

G

K

K

L

A

A

T

T

A

L

L

N

R

A

E

V

F

S

A

A

Q

E

R

L

A

G

G

I

V

T

P

P

M

E

A

L

Q

A

T

V

V

S

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

D

D

M

M

L

L

R

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

A

E

E

V

A

A

R

D

V

A

R

S

V

L

D

S

H

H

G

P

D

Y

L

L

V

D

T

T

L

V

L

L

Y

F

A

L

Q

L

G

G

Y

V

A

T

D

R

D

G

E

E

M

I

binding magnesium ion: D183 (= D90), D185 (= D92), D339 (= D246)
binding 4-phenyl-1h-imidazole: D185 (= D92), E192 (= E99), V193 (≠ T100), I194 (≠ L101), T211 (= T118), M215 (= M122), F221 (= F128), R228 (= R135), G273 (= G180)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
36% identity, 90% coverage: 30:296/298 of query aligns to 117:389/396 of 8a1zA

query
sites
8a1zA

A

A

L

L

G

G

A

V

E

N

V

I

G

D

H

L

A

I

R

R

W

G

L

V

S

S

P

D

E

Y

H

P

A

V

C

I

D

G

L

L

D

E

F

L

S

R

E

V

L

S

D

V

P

P

R

P

Q

G

A

A

D

D

Q

E

V

A

A

L

-

R

-

T

-

A

-

L

A

N

R

R

I

V

L

S

E

S

G

E

W

E

N

H

V

V

D

D

V

V

V

A

A

V

Q

E

-

D

-

Y

K

T

A

L

E

E

G

R

R

R

R

A

K

K

M

R

L

L

L

I

V

V

A

F

D
|
D

M

V

D
|
D

S

S

T

T

M

L

V

V

I

Q

G

G

E
|
E

T

V

L

I

D

E

E

M

L

L

A

A

D

A

F

K

A

A

G

G

L

A

K

E

D

G

H

Q

I

V

A

A

R

A

I

I

T

T

A

D

R

A

A

A

M
|
M

N

R

G

G

E

E

I

L

G

D

F
|
F

E

A

A

Q

A

S

L
|
L

R

Q

E

Q

R
|
R

V

V

G

A

L

T

L

L

K

A

D

G

L

L

P

P

E

A

E

T

C

V

L

I

Q

D

K

E

T

V

W

A

D

G

R

Q

I

L

E

E

F

L

T

M

P

P

G

G

A

A

H

R

K

T

L

T

V

L

R

R

T

T

M

L

V

R

K

R

H

L

G

G

A

Y

H

A

A

C

V

G

L

V

V

V

S

S

G
|
G

G

G

F
|
F

K

R

F

R

F

I

T

I

S

E

K

P

V

L

R

A

D

E

A

E

C

L

G

M

F

L

H

D

R

Y

D

V

I

A

A

A

N

N

E

E

L

L

I

E

V

I

E

V

N

D

G

G

R

T

L

L

T

T

G

G

Q

R

V

V

G

V

D

G

T

P

I

I

G

D

R

R

E

A

A

G

K

K

L

A

A

T

T

A

L

L

N

R

A

E

V

F

S

A

A

Q

E

R

L

A

G

G

I

V

T

P

P

M

E

A

L

Q

A

T

V

V

S

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

D

D

M

M

L

L

R

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

A

E

E

V

A

A

R

D

V

A

R

S

V

L

D

S

H

H

G

P

D

Y

L

L

V

D

T

T

L

V

L

L

Y

F

A

L

Q

L

G

G

Y

V

A

T

D

R

D

G

E

E

M

I

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D92), E192 (= E99), M215 (= M122), F221 (= F128), L225 (= L132), R228 (= R135), G272 (= G179), F274 (= F181), D339 (= D246)
binding magnesium ion: D183 (= D90), D185 (= D92), D339 (= D246)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
36% identity, 90% coverage: 30:296/298 of query aligns to 117:389/396 of 5jlpA

query
sites
5jlpA

A

A

L

L

G

G

A

V

E

N

V

I

G

D

H

L

A

I

R

R

W

G

L

V

S

S

P

D

E

Y

H

P

A

V

C

I

D

G

L

L

D

E

F

L

S

R

E

V

L

S

D

V

P

P

R

P

Q

G

A

A

D

D

-

G

-

A

-

L

-

R

Q

T

V

A

A

L

A

N

R

R

I

V

L

S

E

S

G

E

W

E

N

H

V

V

D

D

V

V

V

A

A

V

Q

E

-

D

-

Y

K

T

A

L

E

E

G

R

R

R

R

A

K

K

M

R

L

L

L

I

V

V

A

F

D
|
D

M
x
V

D
|
D

S

S

T

T

M

L

V

V

I

Q

G

G

E

E

T

V

L

I

D

E

E

M

L

L

A

A

D

A

F

K

A

A

G

G

L

A

K

E

D

G

H

Q

I

V

A

A

R

A

I

I

T

T

A

D

R

A

A

A

M

M

N

R

G

G

E

E

I

L

G

D

F

F

E

A

A

Q

A

S

L

L

R

Q

E

Q

R

R

V

V

G

A

L

T

L

L

K

A

D

G

L

L

P

P

E

A

E

T

C

V

L

I

Q

D

K

E

T

V

W

A

D

G

R

Q

I

L

E

E

F

L

T

M

P

P

G

G

A

A

H

R

K

T

L

T

V

L

R

R

T

T

M

L

V

R

K

R

H

L

G

G

A

Y

H

A

A

C

V

G

L

V

V

V

S

S

G
|
G

G

G

F

F

K

R

F

R

F

I

T

I

S

E

K

P

V

L

R

A

D

E

A

E

C

L

G

M

F

L

H

D

R

Y

D

V

I

A

A

A

N

N

E

E

L

L

I

E

V

I

E

V

N

D

G

G

R

T

L

L

T

T

G

G

Q

R

V

V

G

V

D

G

T

P

I

I

G

D

R

R

E

A

A

G

K
|
K

L

A

A

T

T

A

L

L

N

R

A

E

V

F

S

A

A

Q

E

R

L

A

G

G

I

V

T

P

P

M

E

A

L

Q

A

T

V

V

S

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

D

D

M

M

L

L

R

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

A

E

E

V

A

A

R

D

V

A

R

S

V

L

D

S

H

H

G

P

D

Y

L

L

V

D

T

T

L

V

L

L

Y

F

A

L

Q

L

G

G

Y

V

A

T

D

R

D

G

E

E

M

I

active site: D183 (= D90), V184 (≠ M91), D185 (= D92), G272 (= G179), K316 (= K223), D343 (= D250)
binding magnesium ion: D183 (= D90), D339 (= D246)

Sites not aligning to the query:

binding serine: 13, 14

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
36% identity, 90% coverage: 30:296/298 of query aligns to 121:393/411 of A0QJI1

query
sites
A0QJI1

A

A

L

L

G

G

A

V

E

N

V

I

G

D

H

L

A

I

R

R

W

G

L

V

S

S

P

D

E

Y

H

P

A

V

C

I

D

G

L

L

D

E

F

L

S

R

E

V

L

S

D

V

P

P

R

P

Q

G

A

A

D

D

-

G

-

A

-

L

-

R

Q

T

V

A

A

L

A

N

R

R

I

V

L

S

E

S

G

E

W

E

N

H

V

V

D

D

V

V

V

A

A

V

Q

E

-

D

-

Y

K

T

A

L

E

E

G

R

R

R

R

A

K

K

M

R

L

L

L

I

V

V

A

F

D
|
D

M

V

D
|
D

S

S

T

T

M

L

V

V

I

Q

G

G

E

E

T

V

L

I

D

E

E

M

L

L

A

A

D

A

F

K

A

A

G

G

L

A

K

E

D

G

H

Q

I

V

A

A

R

A

I

I

T

T

A

D

R

A

A

A

M

M

N

R

G

G

E

E

I

L

G

D

F

F

E

A

A

Q

A

S

L

L

R

Q

E

Q

R

R

V

V

G

A

L

T

L

L

K

A

D

G

L

L

P

P

E

A

E

T

C

V

L

I

Q

D

K

E

T

V

W

A

D

G

R

Q

I

L

E

E

F

L

T

M

P

P

G

G

A

A

H

R

K

T

L

T

V

L

R

R

T

T

M

L

V

R

K

R

H

L

G

G

A

Y

H

A

A

C

V

G

L

V

V

V

S

S

G

G

F

F

K

R

F

R

F

I

T

I

S

E

K

P

V

L

R

A

D

E

A

E

C

L

G

M

F

L

H

D

R

Y

D

V

I

A

A

A

N

N

E

E

L

L

I

E

V

I

E

V

N

D

G

G

R

T

L

L

T

T

G

G

Q

R

V

V

G

V

D

G

T

P

I

I

G

D

R

R

E

A

A

G

K

K

L

A

A

T

T

A

L

L

N

R

A

E

V

F

S

A

A

Q

E

R

L

A

G

G

I

V

T

P

P

M

E

A

L

Q

A

T

V

V

S

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

D

D

M

M

L

L

R

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

A

E

E

V

A

A

R

D

V

A

R

S

V

L

D

S

H

H

G

P

D

Y

L

L

V

D

T

T

L

V

L

L

Y

F

A

L

Q

L

G

G

Y

V

A

T

D

R

D

G

E

E

M

I

D187 (= D90) binding
D189 (= D92) binding
D343 (= D246) binding

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
35% identity, 66% coverage: 86:282/298 of query aligns to 5:200/208 of 3m1yC

query
sites
3m1yC

L

L

L

A

V

V

A

F

D
|
D

M
x
F

D
|
D

S

S

T

T

M

L

V

V

I

N

G

A

E

E

T

T

L

I

D

E

E

S

L

L

A

A

D

R

F

A

A

W

G

G

L

V

K

F

D

D

H

E

I

V

A

K

R

T

I

I

T

T

A

L

R

K

A

A

M

M

N

N

G

G

E

T

I

-

G

D

F

F

E

H

A

K

A

S

L

L

R

I

E

L

R

R

V

V

G

S

L

K

L

L

K

K

D

N

L

M

P

P

E

L

E

K

C

L

L

A

Q

K

K

E

T

V

W

C

D

E

R

S

I

L

E

P

F

L

T

F

P

E

G

G

A

A

H

L

K

E

L

L

V

V

R

S

T

A

M

L

V

K

K

E

H

K

G

N

A

Y

H

K

A

V

V

V

L

C

V

F

S

S

G
|
G

G

G

F

F

K

D

F

L

F

A

T

T

S

N

K

H

V

Y

R

R

D

D

A

L

C

L

G

H

F

L

H

D

R

A

D

A

I

F

A

S

N

N

E

T

L

L

I

I

V

V

E

E

N

N

G

D

R

A

L

L

T

N

G

G

Q

L

V

V

G

T

D

G

T

H

I

M

G

F

R

S

E

H

A

S

K
|
K

L

G

A

E

T

M

L

L

N

L

A

V

V

L

S

Q

A

R

E

L

L

L

G

N

I

I

T

S

P

K

E

T

L

N

A

T

V

L

S

V

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

L

D

S

M

M

L

F

R

K

A

H

A

A

G

H

L

I

G

K

V

I

A

A

F

F

H

N

A

A

K

K

P

E

V

V

V

L

A

K

A

Q

E

H

A

A

R

T

V

H

R

C

V

I

D

N

H

E

G

P

D

D

L

L

active site: D9 (= D90), F10 (≠ M91), D11 (= D92), G97 (= G179), K141 (= K223), D168 (= D250)
binding magnesium ion: D9 (= D90), D11 (= D92), D164 (= D246)

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
35% identity, 34% coverage: 83:182/298 of query aligns to 4:109/217 of 6q6jB

query
sites
6q6jB

R

R

K

K

M

L

L

F

L

Y

V

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

V

I

I

R

G

E

E

E

T

G

L

I

D

D

E

E

L

L

A

A

D

K

F

I

A

C

G

G

L

V

K

E

D

D

H

A

I

V

A

S

R

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

G

P

F
|
F

E

K

A

A

L

L

R

T

E

E

R

R

V

L

G

A

L

L

L

I

K

Q

D

P

L

S

P

R

E

E

E

Q

C

V

L

Q

Q

R

K

L

T

I

W

A

D

E

R

Q

I

P

E

P

-

H

F

L

T

T

P

P

G

G

A

I

H

R

K

E

L

L

V

V

R

S

T

R

M

L

V

Q

K

E

H

R

G

N

A

V

H

Q

A

V

V

F

L

L

V

I

S
|
S

G
|
G

F

F

K

R

binding calcium ion: D16 (vs. gap), D18 (= D92)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (vs. gap), V17 (≠ M91), D18 (= D92), F54 (= F128), S105 (= S178), G106 (= G179), G107 (= G180)

Sites not aligning to the query:

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
35% identity, 34% coverage: 83:182/298 of query aligns to 4:109/221 of 6hyyA

query
sites
6hyyA

R

R

K

K

M

L

L

F

L

Y

V

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

V

I

I

R

G

E

E

E

T

G

L

I

D

D

E

E

L

L

A

A

D

K

F

I

A

C

G

G

L

V

K

E

D

D

H

A

I

V

A

S

R

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

G

P

F

F

E

K

A

A

L

L

R

T

E

E

R

R

V

L

G

A

L

L

L

I

K

Q

D

P

L

S

P

R

E

E

E

Q

C

V

L

Q

Q

R

K

L

T

I

W

A

D

E

R

Q

I

P

E

P

-

H

F

L

T

T

P

P

G

G

A

I

H

R

K

E

L

L

V

V

R

S

T

R

M

L

V

Q

K

E

H

R

G

N

A

V

H

Q

A

V

V

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

K

R

binding magnesium ion: D16 (vs. gap), D18 (= D92)
binding serine: D16 (vs. gap), V17 (≠ M91), D18 (= D92), S105 (= S178), G106 (= G179)

Sites not aligning to the query:

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
36% identity, 31% coverage: 90:182/298 of query aligns to 20:113/225 of P78330

query
sites
P78330

D
|
D

M
x
V

D
|
D

S
|
S

T

T

M

V

V

I

I

R

G

E

E
|
E

T

G

L

I

D
|
D

E

E

L

L

A
|
A

D

K

F

I

A

C

G

G

L

V

K

E

D

D

H

A

I

V

A

S

R

E

I

M

T

T

A

R

R

R

A

A

M
|
M

N
x
G

G

G

E

A

I

V

G

P

F

F

E

K

A

A

L

L

R

T

E

E

R
|
R

V

L

G

A

L

L

L

I

K

Q

D

P

L

S

P

R

E

E

E

Q

C

V

L

Q

Q

R

K

L

T

I

W

A

D

E

R

Q

I

P

E

P

-

H

F

L

T

T

P

P

G

G

A

I

H

R

K

E

L

L

V

V

R

S

T

R

M

L

V

Q

K

E

H

R

G

N

A

V

H

Q

A

V

V

F

L

L

V

I

S
|
S

G
|
G

F

F

K

R

D20 (= D90) binding
DVD 20:22 (≠ DMD 90:92) binding
D22 (= D92) binding
S23 (= S93) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E99) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D102) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A105) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M122) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ N123) binding
R65 (= R135) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 178:180) binding ; binding

Sites not aligning to the query:

133 N→A: Reduces L-phosphoserine phosphatase activity by about 75%.
158 binding ; binding
179 binding
182 binding ; binding ; T→S: Reduces L-phosphoserine phosphatase activity by about 99%.; T→V: Reduces L-phosphoserine phosphatase activity by about 25%.
202 R→A: Reduces L-phosphoserine phosphatase activity by about 99%.; R→K: Reduces L-phosphoserine phosphatase activity by about 95%.

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
35% identity, 34% coverage: 83:182/298 of query aligns to 5:110/222 of 1l8oA

query
sites
1l8oA

R

R

K

K

M

L

L

F

L

Y

V

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

V

I

I

R

G

E

E

E

T

G

L

I

D

D

E

E

L

L

A

A

D

K

F

I

A

C

G

G

L

V

K

E

D

D

H

A

I

V

A

S

R

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

G

P

F

F

E

K

A

A

L

L

R

T

E

E

R

R

V

L

G

A

L

L

L

I

K

Q

D

P

L

S

P

R

E

E

E

Q

C

V

L

Q

Q

R

K

L

T

I

W

A

D

E

R

Q

I

P

E

P

-

H

F

L

T

T

P

P

G

G

A

I

H

R

K

E

L

L

V

V

R

S

T

R

M

L

V

Q

K

E

H

R

G

N

A

V

H

Q

A

V

V

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

K

R

active site: D17 (vs. gap), V18 (≠ M91), D19 (= D92), G107 (= G179)
binding phosphate ion: D17 (vs. gap), D19 (= D92), S106 (= S178)

Sites not aligning to the query:

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
35% identity, 34% coverage: 83:182/298 of query aligns to 5:110/222 of 1l8lA

query
sites
1l8lA

R

R

K

K

M

L

L

F

L

Y

V

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

V

I

I

R

G

E

E

E

T

G

L

I

D

D

E

E

L

L

A

A

D

K

F

I

A

C

G

G

L

V

K

E

D

D

H

A

I

V

A

S

R

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

G

P

F

F

E

K

A

A

L

L

R

T

E

E

R

R

V

L

G

A

L

L

L

I

K

Q

D

P

L

S

P

R

E

E

E

Q

C

V

L

Q

Q

R

K

L

T

I

W

A

D

E

R

Q

I

P

E

P

-

H

F

L

T

T

P

P

G

G

A

I

H

R

K

E

L

L

V

V

R

S

T

R

M

L

V

Q

K

E

H

R

G

N

A

V

H

Q

A

V

V

F

L

L

V

I

S

S

G
|
G

G

G

F

F

K

R

active site: D17 (vs. gap), V18 (≠ M91), D19 (= D92), G107 (= G179)
binding d-2-amino-3-phosphono-propionic acid: D17 (vs. gap), D19 (= D92), G107 (= G179)

Sites not aligning to the query:

active site: 155, 180
binding d-2-amino-3-phosphono-propionic acid: 155, 176, 177, 179

6hyjB Psph human phosphoserine phosphatase (see paper)
36% identity, 31% coverage: 90:182/298 of query aligns to 20:113/223 of 6hyjB

query
sites
6hyjB

D
|
D

M

V

D
|
D

S

S

T

T

M

V

V

I

I

R

G

E

E
|
E

T

G

L

I

D

D

E

E

L

L

A

A

D

K

F

I

A

C

G

G

L

V

K

E

D

D

H

A

I

V

A

S

R

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

G

P

F

F

E

K

A

A

L

L

R

T

E

E

R

R

V

L

G

A

L

L

L

I

K

Q

D

P

L

S

P

R

E

E

E

Q

C

V

L

Q

Q

R

K

L

T

I

W

A

D

E

R

Q

I

P

E

P

-

H

F

L

T

T

P

P

G

G

A

I

H

R

K

E

L

L

V

V

R

S

T

R

M

L

V

Q

K

E

H

R

G

N

A

V

H

Q

A

V

V

F

L

L

V

I

S

S

G

G

F

F

K

R

binding calcium ion: D20 (= D90), D22 (= D92)
binding serine: E29 (= E99)

Sites not aligning to the query:

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
25% identity, 60% coverage: 84:262/298 of query aligns to 9:174/200 of 4ap9A

query
sites
4ap9A

K

K

M

K

L

V

L

A

V

V

A

I

D
|
D

M
x
I

D
x
E

S

G

T

T

M

L

V

T

I

D

G

F

E

E

T

F

L

W

D

R

E

E

L

M

A

A

D
x
R

F
x
I

A
x
T

G

G

L

-

K

K

D

R

H

E

I

I

A

E

R

E

I

L

T

L

A

E

R

K

A

G

M
x
L

N

S

G

G

E

E

I

V

G

E

F
x
W

E

L

A

D

A

S

L

L

R

L

E

K

R

R

V

V

G

G

L

L

L

I

K

R

D

G

L

I

P

D

E

E

E

G

C

T

L

F

Q

L

K

R

T

T

W

R

D

E

R

K

I

V

E

N

F

V

T

S

P

P

G

E

A

A

H

R

K

E

L

L

V

V

R

E

T

T

M

L

V

R

K

E

H

K

G

G

A

F

H

K

A

V

V

V

L

L

V

I

S

S

G
|
G

G

S

F

F

K

E

F

E

F

V

T

L

S

E

K

P

V

F

R

K

D

E

A

L

C

G

G

-

F

-

H

D

R

E

D

F

I

M

A

A

N

N

E

R

L

A

I

I

V

F

E

E

N

D

G

G

R

K

L

F

T

Q

G

G

Q

-

V

-

G

-

D

-

T

-

I

-

G

-

R

-

E

-

A

I

K

R

L

L

A

R

T

F

L

R

N
x
D

A
x
K

V

G

S

E

A

F

E

L

L

K

G

R

I

F

T

R

P

D

E

G

L

F

A

I

V

L

S

A

V

M

G

G

D

D

G

G

A
x
Y

N
x
A

D
|
D

L

A

D

K

M

M

L

F

R

E

A

R

A

A

G

D

L

M

G

G

V

I

A

A

active site: D15 (= D90), I16 (≠ M91), E17 (≠ D92), G103 (= G179), K141 (≠ A229), D162 (= D250)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ D106), I32 (≠ F107), T33 (≠ A108), L46 (≠ M122), W52 (≠ F128), D140 (≠ N228), K141 (≠ A229), Y160 (≠ A248), A161 (≠ N249)

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
24% identity, 62% coverage: 90:273/298 of query aligns to 20:209/585 of 6iuyA

query
sites
6iuyA

D
|
D

M

V

D
|
D

S

R

T

T

M

V

V

T

I

T

G

D

E

A

T

S

L

V

D

G

E

L

L

L

A

A

D

K

F

F

A

M

G

G

L

I

K

E

D

D

H

E

I

A

A

Q

R

S

I

L

T

T

A

E

R

Q

A

A

M

N

N

R

G

G

E

E

I

I

G

N

F

L

E

T

A

K

A

A

L

F

R

E

E

D

R

R

V

L

G

A

L

K

L

L

K

N

D

F

L

T

P

P

E

T

E

D

C

I

-

D

-

R

-

F

L

L

Q

E

K

E

T

H

W

P

D

A

R

H

I

T

E

R

F

L

T

V

P

P

G

G

A

V

H

E

K

N

L

L

V

I

R

A

T

A

M

L

V

K

K

A

H

R

G

G

A

V

H

E

A

V

V

F

L

L

V

I

S

S

G

G

F

F

K

R

F

E

F

M

T

A

S

L

K

P

V

I

R

A

D

S

A

-

C

-

G

-

F

-

H

H

R

L

D

K

I

I

A

P

N

A

E

K

L

N

I

V

V

F

E

C

N

N

G

-

R

T

L

M

T

S

G

W

Q

Q

V

L

G

D

D

D

T

H

I

-

G

G

R

E

E

P

A

V

K

R

L

L

A

S

T

H

L

F

N

K

A

S

V

R

S

A

A

I

E

E

-

R

-

I

-

R

L

R

G

K

I

Y

T

P

P

Y

E

N

L

N

A

I

V

I

S

M

V

V

G

G

D
|
D

G

G

A

F

N

S

D

D

L

L

D

E

M

A

L

M

R

Q

A

G

A

S

G

P

L

D

G

G

-

A

-

D

-

A

-

F

V

I

A

C

F

F

-

G

-

V

H

M

A

Q

K

R

P

P

V

A

V

V

A

A

A

S

E

Q

A

A

binding magnesium ion: D20 (= D90), D22 (= D92), D175 (= D246)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

Query Sequence

>WP_011385839.1 NCBI__GCF_000009985.1:WP_011385839.1
MINVLTLIAGHGGEGLDSSLVFEVRGALRALGAEVGHARWLSPEHACDLDFSELDPRQAD
QVAARILEGWNVDVVAQKAEGRRKMLLVADMDSTMVIGETLDELADFAGLKDHIARITAR
AMNGEIGFEAALRERVGLLKDLPEECLQKTWDRIEFTPGAHKLVRTMVKHGAHAVLVSGG
FKFFTSKVRDACGFHRDIANELIVENGRLTGQVGDTIIGREAKLATLNAVSAELGITPEL
AVSVGDGANDLDMLRAAGLGVAFHAKPVVAAEARVRVDHGDLVTLLYAQGYADDEMVE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory