SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011386084.1 NCBI__GCF_000009985.1:WP_011386084.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

Q5F8J4 NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
44% identity, 95% coverage: 24:428/428 of query aligns to 22:433/435 of Q5F8J4

query
sites
Q5F8J4

L

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L45 (= L47) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ LA 47:48) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
44% identity, 99% coverage: 3:426/428 of query aligns to 2:429/431 of 6dzsA

query
sites
6dzsA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), L12 (= L13), G13 (= G14), N14 (≠ T15), V15 (= V16), V45 (≠ A46), R46 (≠ L47), R47 (≠ A48), R52 (≠ L53), I63 (vs. gap), L78 (= L79), M79 (≠ I80), P84 (≠ G85), A102 (= A104), K104 (= K106), G306 (= G303), T310 (= T307)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
36% identity, 78% coverage: 6:340/428 of query aligns to 5:331/402 of 4pg7A

query
sites
4pg7A

L

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S

active site: D190 (= D202), K194 (= K206)
binding lysine: R215 (≠ E227), G219 (≠ N231)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 75% coverage: 6:324/428 of query aligns to 4:311/332 of 6a0tB

query
sites
6a0tB

L

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K

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R

G

A

V

E

P

P

F

F

F

-

T

-

D

-

A

-

R

-

D

-

M

-

A

-

K

D

T

L

C

L

D

E

Y

A

D

D

V

L

L

V

V

V

E

E

L
x
A

I
x
M

G
|
G

G

G

A

V

K

E

G

A

I

-

A

P

L

L

D

R

V

L

V

V

R

L

T

P

A

A

L

L

E

E

R

A

G

G

K

I

S

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

M

L

I

L

A

A

H

E

H

A

G

W

I

E

E

S

L

L

A

R

R

P

I

F

A

A

E

E

A

E

K

-

G

-

G

G

N

L

I

I

G

Y

F

H

E

E

A

A

S

S

V

V

G

M

G

A

G

G

I

T

P

P

V

A

I

L

K

-

S

S

L

F

R

L

E

E

G

T

L

L

A

R

G

G

N

S

R

E

V

L

T

L

K

E

V

L

M

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

T

E

M

M

R

-

D

E

S

K

G

G

R

R

D

T

F

Y

S

A

D

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

S

T

F

L

D
|
D

I

V

D

E

G

G

T

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

A

A

S

R

L

L

A

L

F

V

A

D

M

P

P

G

L

F

D

P

I

F

D

A

A

E

V

V

S

E

C

A

E

Q

G

G

I

I

R

A

N

R

V

L

S

T

A

P

L

E

D

V

I

L

R

Q

Y

K

A

A

D

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

S

S

L

L

S

F

D

G

Q

E

G

G

V

G

E

R

T

W

R

R

-

A

V

V

Y

A

P

P

A

R

M

R

V

L

P

P

L

Q

S

D

F

H

P

P

L

L

A

A

H

R

V

A

S

R

G

G

P

-

F

-

N

N

A

A

I

L

V

W

T

V

E

R

G

A

D

R

F

P

V

L

G

G

R

E

T

A

V

F

L

V

E

T

G

G

R

P

G

G

A
|
A

G
|
G

A

G

R

G

P

A

T
|
T

A

A

S

S

A

G

V

L

V

F

A

A

D

D

L

L

M

L

D

R

L

F

A

L

T

S

G

G

R

A

V

P

G

G

active site: D191 (= D202), K195 (= K206)
binding l-homoserine: N150 (= N160), G151 (= G161), T152 (= T162), Y178 (= Y189), E180 (= E191), D186 (= D197), K195 (= K206)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G12), G11 (≠ L13), G12 (= G14), T13 (= T15), V14 (= V16), L42 (≠ G43), V43 (≠ A44), R44 (≠ A45), D45 (≠ A46), K48 (= K49), R50 (≠ A51), A73 (≠ L79), M74 (≠ I80), G75 (= G81), A97 (= A104), N98 (= N105), G177 (= G188), E180 (= E191), A289 (= A302), G290 (= G303), T294 (= T307)

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 75% coverage: 6:324/428 of query aligns to 4:311/331 of 6a0sA

query
sites
6a0sA

L

L

K

K

I

I

A

A

I

L

A

L

G

G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

G

S

G

A

T

F

V

Y

Q

N

L

L

L

V

H

L

D

E

H

R

A

A

P

E

L

E

L

L

A

S

A

A

R

F

A

G

G

V

R

V

P

P

L

R

A

F

L

L

-

G

V

V

G
x
L

A
x
V

A
x
R

A
x
D

L

P

A

R

K
|
K

P

P

A
x
R

D

A

L

I

P

P

-

Q

-

E

-

L

L

L

D

R

G

A

V

E

P

P

F

F

F

-

T

-

D

-

A

-

R

-

D

-

M

-

A

-

K

D

T

L

C

L

D

E

Y

A

D

D

V

L

L

V

V

V

E

E

L
x
A

I
x
M

G

G

A

V

K

E

G

A

I

-

A

P

L

L

D

R

V

L

V

V

R

L

T

P

A

A

L

L

E

E

R

A

G

G

K

I

S

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

M

L

I

L

A

A

H

E

H

A

G

W

I

E

E

S

L

L

A

R

R

P

I

F

A

A

E

E

A

E

K

-

G

-

G

G

N

L

I

I

G

Y

F

H

E

E

A

A

S

S

V

V

G

M

G

A

G

G

I

T

P

P

V

A

I

L

K

-

S

S

L

F

R

L

E

E

G

T

L

L

A

R

G

G

N

S

R

E

V

L

T

L

K

E

V

L

M

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

T

E

M

M

R

-

D

E

S

K

G

G

R

R

D

T

F

Y

S

A

D

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

S

T

F

L

D
|
D

I

V

D

E

G

G

T

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

A

A

S

R

L

L

A

L

F

V

A

D

M

P

P

G

L

F

D

P

I

F

D

A

A

E

V

V

S

E

C

A

E

Q

G

G

I

I

R

A

N

R

V

L

S

T

A

P

L

E

D

V

I

L

R

Q

Y

K

A

A

D

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

S

S

L

L

S

F

D

G

Q

E

G

G

V

G

E

R

T

W

R

R

-

A

V

V

Y

A

P

P

A

R

M

R

V

L

P

P

L

Q

S

D

F

H

P

P

L

L

A

A

H

R

V

A

S

R

G

G

P

-

F

-

N

N

A

A

I

L

V

W

T

V

E

R

G

A

D

R

F

P

V

L

G

G

R

E

T

A

V

F

L

V

E

T

G

G

R

P

G

G

A
|
A

G
|
G

A

G

R

G

P

A

T
|
T

A

A

S

S

A

G

V

L

V

F

A

A

D

D

L

L

M

L

D

R

L

F

A

L

T

S

G

G

R

A

V

P

G

G

active site: D191 (= D202), K195 (= K206)
binding l-homoserine: K99 (= K106), N150 (= N160), G151 (= G161), T152 (= T162), Y178 (= Y189), E180 (= E191), D186 (= D197), K195 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L13), G12 (= G14), T13 (= T15), V14 (= V16), L42 (≠ G43), V43 (≠ A44), R44 (≠ A45), D45 (≠ A46), K48 (= K49), R50 (≠ A51), A73 (≠ L79), M74 (≠ I80), A97 (= A104), K99 (= K106), G177 (= G188), E180 (= E191), A289 (= A302), G290 (= G303), T294 (= T307)

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 75% coverage: 6:324/428 of query aligns to 4:311/331 of 2ejwA

query
sites
2ejwA

L

L

K

K

I

I

A

A

I

L

A

L

G

G

L

G

G

G

T

T

V

V

G

G

G

S

G

A

T

F

V

Y

Q

N

L

L

L

V

H

L

D

E

H

R

A

A

P

E

L

E

L

L

A

S

A

A

R

F

A

G

G

V

R

V

P

P

L

R

A

F

L

L

-

G

V

V

G

L

A

V

A

R

A

D

L

P

A

R

K

K

P

P

A

R

D

A

L

I

P

P

-

Q

-

E

-

L

L

L

D

R

G

A

V

E

P

P

F

F

F

-

T

-

D

-

A

-

R

-

D

-

M

-

A

-

K

D

T

L

C

L

D

E

Y

A

D

D

V

L

L

V

V

V

E

E

L

A

I

M

G

G

A

V

K

E

G

A

I

-

A

P

L

L

D

R

V

L

V

V

R

L

T

P

A

A

L

L

E

E

R

A

G

G

K

I

S

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

M

L

I

L

A

A

H

E

H

A

G

W

I

E

E

S

L

L

A

R

R

P

I

F

A

A

E

E

A

E

K

-

G

-

G

G

N

L

I

I

G

Y

F

H

E
|
E

A

A

S

S

V
|
V

G
x
M

G
x
A

G

G

I
x
T

P

P

V

A

I

L

K

-

S

S

L

F

R

L

E

E

G

T

L

L

A

R

G

G

N

S

R

E

V

L

T

L

K

E

V

L

M

H

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

L

Y

Y

I

I

L

L

T

Q

T

E

M

M

R

-

D

E

S

K

G

G

R

R

D

T

F

Y

S

A

D

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

S

T

F

L

D

D

I

V

D

E

G

G

T

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

A

A

S

R

L

L

A

L

F

V

A

D

M

P

P

G

L

F

D

P

I

F

D

A

A

E

V

V

S

E

C

A

E

Q

G

G

I

I

R

A

N

R

V

L

S

T

A

P

L

E

D

V

I

L

R

Q

Y

K

A

A

D

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

S

S

L

L

S

F

D

G

Q

E

G

G

V

G

E

R

T

W

R

R

-

A

V

V

Y

A

P

P

A

R

M

R

V

L

P

P

L

Q

S

D

F

H

P

P

L

L

A

A

H

R

V

A

S

R

G

G

P

-

F

-

N

N

A

A

I

L

V

W

T

V

E

R

G

A

D

R

F

P

V

L

G

G

R

E

T

A

V

F

L

V

E

T

G

G

R

P

G

G

A

A

G

G

A

G

R

G

P

A

T

T

A

A

S

S

A

G

V

L

V

F

A

A

D

D

L

L

M

L

D

R

L

F

A

L

T

S

G

G

R

A

V

P

G

G

active site: D191 (= D202), K195 (= K206)
binding magnesium ion: E121 (= E130), V124 (= V133), M125 (≠ G134), A126 (≠ G135), T128 (≠ I137)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
37% identity, 54% coverage: 87:316/428 of query aligns to 83:295/302 of 5x9dA

query
sites
5x9dA

A

S

L

L

D

S

V

L

V

Y

R

K

T

E

A

A

L

I

E

K

R

D

G

G

K

V

S

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

M

P

I

L

A

A

H

L

H

A

G

F

I

N

E

E

L

I

A

F

R

S

I

L

A

A

E

R

A

S

K

K

G

G

G

V

N

K

I

I

G

G

F

F

E

Q

A
x
G

S

T

V

V

G

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

-

S

N

L

L

R

Y

E

R

G

V

L

L

A

P

G

G

N

S

R

R

V

V

T

I

K

K

V

I

M

R

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

I

I

L

L

T

T

T

L

M

M

R

-

D

N

S

K

G

G

R

V

D

S

F

F

S

E

D

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

S

T

F

L

D
|
D

I

I

D

N

G

G

T

F

D
|
D

T

A

A

A

H

A

K
|
K

L

I

A

T

I

I

L

L

A

A

S

N

L

F

A

M

F

I

A

G

M

N

P

S

L

V

D

T

I

I

D

K

A

D

V

V

S

K

C

F

E

E

G

G

I

I

R

N

N

R

V

-

S

-

A

-

L

-

D

-

I

-

R

-

Y

-

A

-

D

D

E

P

L

K

G

N

Y

E

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

S

D

L

-

S

-

D

-

Q

-

G

-

V

-

E

E

T

K

R

E

V

V

Y

W

P

V

A

K

M

P

V

L

P

P

L

I

S

S

F

Q

-

D

P

P

L

L

A

Y

H

N

V

V

S

D

G

G

P

V

F

E

N

N

A

A

I

L

V

E

T

I

E

T

G

T

D

D

F

-

V

I

G

Q

R

S

T

I

V

L

L

I

E

R

G

G

R

P

G

G

A
|
A

G
|
G

A

P

R

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

M

I

active site: D196 (= D202), K200 (= K206)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: T100 (≠ A104), N101 (= N105), K102 (= K106), G127 (≠ A131), S131 (≠ G135), N155 (= N160), G156 (= G161), T157 (= T162), Y183 (= Y189), A184 (= A190), E185 (= E191), D191 (= D197), D196 (= D202), K200 (= K206), A281 (= A302), G282 (= G303), A286 (≠ T307)

Sites not aligning to the query:

binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38, 72, 73, 74, 82

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
37% identity, 54% coverage: 87:316/428 of query aligns to 83:293/300 of 7f4cA

query
sites
7f4cA

A

S

L

L

D

S

V

L

V

Y

R

K

T

E

A

A

L

I

E

K

R

D

G

G

K

V

S

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

M

P

I

L

A

A

H

L

H

A

G

F

I

N

E

E

L

I

A

F

R

S

I

L

A

A

E

R

A

S

K

K

G

G

G

V

N

K

I

I

G

G

F

F

E

Q

A
x
G

S

T

V

V

G

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

-

S

N

L

L

R

Y

E

R

G

V

L

L

A

P

G

G

N

S

R

R

V

V

T

I

K

K

V

I

M

R

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

T

L

M

M

R

-

D

N

S

K

G

G

R

V

D

S

F

F

S

E

D

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

S

T

F

L

D

D

I

I

D

N

G

G

T

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

I

I

L

L

A

A

S

N

L

F

A

M

F

I

A

G

M

N

P

S

L

V

D

T

I

I

D

K

A

D

V

V

S

K

C

F

E

E

G

G

I

I

-

N

R

R

N

D

V

L

S

K

A

I

L

K

D

L

I

I

R

A

Y

Y

A

A

D

D

E

E

L

-

G

-

Y

-

R

-

I

-

K

-

L

-

G

-

V

-

A

-

S

-

L

-

S

-

D

-

Q

-

G

-

V

K

E

E

T

V

R

W

V

V

Y

K

P

P

A

L

M

P

V

I

P

S

L

Q

S

D

F

D

P

P

L

L

A

Y

H

N

V

V

S

D

G

G

P

V

F

E

N

N

A

A

I

L

V

E

T

I

E

T

G

T

D

D

F

-

V

I

G

Q

R

S

T

I

V

L

L

I

E

R

G

G

R

P

G

G

A

A

G
|
G

A

P

R

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

M

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: T100 (≠ A104), K102 (= K106), G127 (≠ A131), S131 (≠ G135), E185 (= E191), G280 (= G303), A284 (≠ T307)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39, 72, 73, 74

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
30% identity, 73% coverage: 6:316/428 of query aligns to 3:309/319 of 4xb1A

query
sites
4xb1A

L

V

K

N

I

I

A

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

G

R

G

A

T

L

V

A

Q

E

L

I

H

A

D

E

H

K

A

S

P

R

L

I

L

F

A

G

A

V

R

E

A

L

G

N

R

V

P

I

L

S

A

I

L

T

V
x
D

G
x
R

A

S

A

G

A

T

L

I

A

W

K

G

P

D

A

F

D

D

L

L

P

-

L

L

D

E

G

A

V

K

P

E

F

V

F
x
K

T

E

D

S

A

T

R

G

D

K

M

L

A

S

K

N

T

I

C

G

D

D

Y

Y

D

E

V

V

L

Y

-

N

-

F

-

S

-

P

V

Q

E

E

L

L

I

V

G

E

A

V

K

K

-

P

G

N

I

I

A

L

L

V

D

D

V
|
V

-
x
S

-

W

-

D

-

E

-

A

-

H

-

E

-

M

V

Y

R

K

T

V

A

A

L

L

E

G

R

E

G

G

K

I

S

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

M

P

I

I

A

A

H

N

H

Y

G

Y

I

D

E

E

L

L

A

M

R

N

I

L

A

A

E

K

A

E

K

N

G

N

G

A

N

G

I

I

G

F

F

F

E

E

A
x
S

S

T

V

V

G

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

S

V

L

L

R

R

E

E

G

N

L

L

A

L

G

G

N

E

R

N

V

I

T

K

K

R

V

I

M

D

G

A

I

V

L

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

T

K

M

M

R

S

D

E

S

-

G

G

R

K

D

T

F

L

S

D

D

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

S

S

F

K

D

D

I

I

D

D

G

G

T

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

I

I

L

L

A

H

S

W

L

V

A

S

F

Y

A

G

M

E

P

P

L

P

D

E

I

E

D

E

A

-

V

-

S

E

C

R

E

L

G

G

I

I

R

R

N

E

V

V

S

R

A

-

L

-

D

D

I

A

R

R

Y

-

A

-

D

-

E

-

L

-

G

-

Y

-

R

N

I

V

K

R

L

L

L

V

G

A

V

Q

A

V

S

S

L

K

S

G

D

K

Q

I

G

S

V

V

E

K

T

P

R

R

V

K

Y

L

P

S

A

S

M

D

V

N

P

P

L

L

S

-

F

-

P

-

L

-

A

-

H

L

V

V

S

E

G

G

P

V

F

Q

N

N

A

A

I

A

V

V

T

I

E

R

G

T

D

N

F

N

V

L

G

G

R

E

T

V

V

I

L

L

E

K

G

G

R

P

G

G

A
x
G

G

G

A

G

R

R

P

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

M

I

active site: D211 (= D202), K215 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A11), F10 (≠ L13), G11 (= G14), T12 (= T15), V13 (= V16), D39 (≠ V42), R40 (≠ G43), K57 (≠ F61), V91 (= V90), S92 (vs. gap), S93 (vs. gap), S114 (≠ A104), K116 (= K106), S141 (≠ A131), G295 (≠ A302), T300 (= T307)

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
30% identity, 73% coverage: 6:316/428 of query aligns to 3:309/319 of 4xb2A

query
sites
4xb2A

L

V

K

N

I

I

A

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

G

R

G

A

T

L

V

A

Q

E

L

I

H

A

D

E

H

K

A

S

P

R

L

I

L

F

A

G

A

V

R

E

A

L

G

N

R

V

P

I

L

S

A

I

L

T

V

D

G
x
R

A

S

A

G

A

T

L

I

A

W

K

G

P

D

A

F

D

D

L

L

P

-

L

L

D

E

G

A

V

K

P

E

F

V

F

A

T

E

D

S

A

T

R

G

D

K

M

L

A

S

K

N

T

I

C

G

D

D

Y

Y

D

E

V

V

L

Y

-

N

-

F

-

S

-

P

V

Q

E

E

L

L

I

V

G

E

A

V

K

K

-

P

G

N

I

I

A

L

L

V

D

D

V
|
V

-
x
S

-

W

-

D

-

E

-

A

-

H

-

E

-

M

V

Y

R

K

T

V

A

A

L

L

E

G

R

E

G

G

K

I

S

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

M

P

I

I

A

A

H

N

H

Y

G

Y

I

D

E

E

L

L

A

M

R

N

I

L

A

A

E

K

A

E

K

N

G

N

G

A

N

G

I

I

G

F

F

F

E

E

A
x
S

S

T

V

V

G

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

S

V

L

L

R

R

E

E

G

N

L

L

A

L

G

G

N

E

R

N

V

I

T

K

K

R

V

I

M

D

G

A

I

V

L

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

I

I

L

L

T

T

T

K

M

M

R

S

D

E

S

-

G

G

R

K

D

T

F

L

S

D

D

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

S

S

F

K

D
|
D

I

I

D

D

G

G

T

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

I

I

L

L

A

H

S

W

L

V

A

S

F

Y

A

G

M

E

P

P

L

P

D

E

I

E

D

E

A

-

V

-

S

E

C

R

E

L

G

G

I

I

R

R

N

E

V

V

S

R

A

-

L

-

D

D

I

A

R

R

Y

-

A

-

D

-

E

-

L

-

G

-

Y

-

R

N

I

V

K

R

L

L

L

V

G

A

V

Q

A

V

S

S

L

K

S

G

D

K

Q

I

G

S

V

V

E

K

T

P

R

R

V

K

Y

L

P

S

A

S

M

D

V

N

P

P

L

L

S

-

F

-

P

-

L

-

A

-

H

L

V

V

S

E

G

G

P

V

F

Q

N

N

A

A

I

A

V

V

T

I

E

R

G

T

D

N

F

N

V

L

G

G

R

E

T

V

V

I

L

L

E

K

G

G

R

P

G

G

A
x
G

G

G

A

G

R

R

P

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

M

I

active site: D211 (= D202), K215 (= K206)
binding l-homoserine: A171 (≠ G161), S172 (≠ T162), D206 (= D197), K215 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A11), F10 (≠ L13), G11 (= G14), T12 (= T15), V13 (= V16), R40 (≠ G43), V91 (= V90), S92 (vs. gap), S93 (vs. gap), S114 (≠ A104), N115 (= N105), K116 (= K106), S141 (≠ A131), G295 (≠ A302), T300 (= T307)

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 75% coverage: 1:320/428 of query aligns to 553:910/916 of O81852

query
sites
O81852

M

L

S

S

K

R

A

T

P

T

L

L

K

A

I

M

A

G

I

I

A

V

G

G

L

P

G

G

T

L

V

I

G

G

G

A

G

T

T

L

V

L

Q

D

L

Q

L

L

H

R

D

D

H

Q

A

A

P

A

L

V

L

L

A

K

A

Q

R

E

A

F

G

N

R

I

P

D

L

L

A

R

L

V

V

L

G

G

A

I

A

T

A

G

L

S

A

K

K

K

P

M

-

L

A

S

D

D

L

I

P

G

L

I

D

D

G

L

V

S

P

R

F

W

F

R

T

E

D

L

A

L

R

N

D

E

M

K

A

G

K

T

T

E

C

A

D

D

Y

L

D

D

V

K

L

F

V

T

E

Q

L

Q

I

V

G

H

G

G

A

N

K

H

G

F

I

I

A

P

L

N

D

S

V

V

-

V

-

D

-

C

-

T

-

A

-

D

-

S

-

A

-

I

-

A

-

S

-

R

-

Y

R

Y

T

D

A

W

L

L

E

R

R

K

G

G

K

I

S

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

M

-

I

-

A

A

H

N

H

S

G

G

-

P

-

L

-

D

-

Q

-

Y

I

L

E

K

L

L

A

R

R

D

I

L

A

Q

E

R

A

K

K

S

G

Y

G

T

N

H

I

Y

G

F

F

Y

E

E

A

A

S

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

T

L

L

R

R

E

G

G

L

L

L

-

E

A

T

G

G

N

D

R

K

V

I

T

L

K

R

V

I

M

E

G

G

I

I

L

C

N

S

G

G

T

T

C

L

N

S

Y

Y

I

L

L

F

T

N

M

F

R

V

D

-

S

G

G

D

R

R

D

S

F

F

S

S

D

E

V

V

L

V

A

T

E

E

A

A

Q

K

A

N

L

A

G

G

Y

F

A

T

E

E

A

P

D

D

P

P

S

R

F

D

D

D

I

L

D

S

G

G

T

T

D

D

T

V

A

A

H

R

K

K

L

V

A

I

I

I

L

L

A

A

S

R

L

E

A

S

F

-

A

G

M

L

P

K

L

L

D

D

-

L

-

A

-

D

-

L

-

P

I

I

D

R

A

S

V

L

S

V

C

P

E

E

G

P

I

L

R

K

N

G

V

C

S

T

A

S

L

V

D

E

I

E

-

F

-

M

-

E

-

K

-

L

-

P

R

Q

Y

Y

-

D

-

G

-

D

-

L

-

A

-

K

-

E

-

R

-

L

-

D

A

A

D

E

E

N

L

S

G

G

Y

E

R

V

I

L

K

R

L

Y

L

V

G

G

V

V

A

V

S

D

L

A

S

V

D

N

Q

Q

G

K

-

G

-

T

V

V

E

E

T

L

R

R

V

R

Y

Y

P

K

A

K

M

-

V

-

P

-

L

-

S

E

F

H

P

P

L

F

A

A

H

Q

V

L

S

A

G

G

P

S

F

D

N

N

A

I

I

I

-

A

V

F

T

T

E

T

G

T

D

R

F

Y

V

K

G

D

R

H

-

P

T

L

V

I

L

V

E

R

G

G

R

P

G

G

A

A

G

G

A

A

R

Q

P

V

T

T

A

A

S

G

A

G

V

I

V

F

A

S

D

D

L

I

M

L

D

R

L

L

A

A

T

S

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
33% identity, 32% coverage: 92:229/428 of query aligns to 100:236/319 of 3ingA

query
sites
3ingA

R

R

T

M

A

A

L

F

E

E

R

S

G

G

K

M

S

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

M

G

I

L

A

A

H

N

H

K

G

W

I

H

E

D

L

I

A

M

R

D

I

S

A

A

E

N

A

Q

K

N

G

S

G

K

N

Y

I

I

G

R

F

Y

E

E

A
|
A

S

T

V

V

G

A

G

G

I

V

P

P

V

L

I

F

K

S

S

V

L

L

R

D

E

Y

G

S

L

I

A

L

G

P

N

S

R

K

V

V

T

K

K

R

V

F

M

R

G

G

I

I

L

V

N

S

G

S

T

T

C

I

N

N

Y

Y

I

V

L

I

T

R

T

N

M

M

R

A

D

N

S

-

G

G

R

R

D

S

F

L

S

R

D

D

V

V

L

V

A

D

E

D

A

A

Q

I

A

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

S

Q

F

D

D

D

I

L

D

N

G

G

T

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

I

I

L

L

A

V

S

N

L

H

A

I

F

F

A

G

M

T

P

E

L

Y

D

T

I

L

D

N

A

D

V

V

S

E

C

Y

E

S

G

G

I

V

active site: D209 (= D202), K213 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A112 (= A104), N113 (= N105), K114 (= K106), A139 (= A131), E198 (= E191), S199 (≠ A192)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 38, 39, 57, 85, 86, 87, 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
27% identity, 52% coverage: 6:229/428 of query aligns to 4:239/321 of 3jsaA

query
sites
3jsaA

L

I

K

N

I

L

A

S

I

I

A

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

G

L

G

N

T

L

V

L

Q

R

L

I

H

R

D

S

H

F

A

N

P

E

L

-

A

E

A

N

R

R

A

L

G

G

R

L

P

K

L

F

A

N

L

V

V

V

G

F

A

V

A

A

-
x
D

-
x
S

-

L

-

H

-

S

-

Y

-

N

-

E

-

R

-

I

-

D

-

I

-

G

-

K

A

V

L

I

A

S

K

Y

P

K

A

E

D

K

L

G

P

S

L

L

D

D

G

S

V

L

P

E

F

Y

F

E

T

S

D

I

A

S

R

A

D

S

M

E

A

A

K

L

T

A

C

R

D

D

Y

F

D

D

V

I

L

V

V

V

E

D

L
x
A

I
x
T

-
x
P

-

A

-

S

-

A

G

D

G

G

A

K

K

K

G

E

I

L

A

A

L

F

D

-

V

-

V

Y

R

K

T

E

A

T

L

F

E

E

R

N

G

G

K

K

S

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

M

G

I

L

A

A

H

N

H

F

G

W

I

P

E

E

L

I

A

M

R

E

I

Y

A

A

E

R

A

S

K

N

G

N

G

R

N

R

I

I

G

R

F

Y

E

E

A
|
A

S

T

V

V

G

A

G

G

I

V

P

P

V

L

I

F

K

S

S

F

L

I

R

D

E

Y

G

S

L

V

A

L

G

P

N

S

R

R

V

I

T

K

K

K

V

F

M

R

G

G

I

I

L

V

N

S

G

L

T

T

C

I

N

N

Y

Y

I

F

L

I

T

R

T

E

M

L

R

A

D

N

S

K

G

-

R

R

D

E

F

F

S

D

D

D

V

V

L

L

A

S

E

E

A

A

Q

T

A

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

S

-

F

-

D

D

I

L

D

T

G

G

T

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

I

I

L

L

A

C

S

N

L

H

A

L

F

Y

A

G

M

S

P

S

L

Y

D

R

I

L

D

S

A

D

V

V

S

F

C

Y

E

E

G

G

I

I

active site: D212 (= D202), K216 (= K206)
binding nicotinamide-adenine-dinucleotide: G10 (= G12), L11 (= L13), G12 (= G14), N13 (≠ T15), V14 (= V16), D42 (vs. gap), S43 (vs. gap), A90 (≠ L79), T91 (≠ I80), P92 (vs. gap), A117 (= A104), N118 (= N105), A144 (= A131)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
37% identity, 28% coverage: 95:214/428 of query aligns to 105:230/358 of 1tveA

query
sites
1tveA

L

V

E

E

R

N

G

G

K

I

S

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

M

A

I

F

A

S

H

S

H

D

G

L

I

A

E

T

L

W

A

K

R

A

I

L

A

F

E

S

A

N

K

K

G

P

G

T

N

N

I

-

G

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

F

L

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

V

V

T

E

K

K

V

I

M

E

G

G

I

I

L

F

N

S

G

G

T
|
T

C

L

N

S

Y

Y

I

I

L

F

T

N

T

E

M

F

R

S

D

T

S

S

G

Q

R

A

D

N

-

D

-

V

-

K

F

F

S

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

S

R

F

D

D
|
D

I

L

D

N

G

G

T

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

A

G

S

R

L

I

A

S

active site: D218 (= D202), K222 (= K206)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K106), T175 (= T162), D213 (= D197), D218 (= D202)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
37% identity, 28% coverage: 95:214/428 of query aligns to 105:230/358 of 1q7gA

query
sites
1q7gA

L

V

E

E

R

N

G

G

K

I

S

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

M

A

I

F

A

S

H

S

H

D

G

L

I

A

E

T

L

W

A

K

R

A

I

L

A

F

E

S

A

N

K

K

G

P

G

T

N

N

I

-

G

G

F

F

-

V

-

Y

-

H

E

E

A
|
A

S

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

F

L

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

V

V

T

E

K

K

V

I

M

E

G

G

I

I

L

F

N
x
S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

T

E

M

F

R

S

D

T

S

S

G

Q

R

A

D

N

-

D

-

V

-

K

F

F

S

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

S

R

F

D

D

D

I

L

D

N

G

G

T

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

A

G

S

R

L

I

A

S

active site: D218 (= D202), K222 (= K206)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A104), K116 (= K106), A143 (= A131), S173 (≠ N160), K222 (= K206)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
37% identity, 28% coverage: 95:214/428 of query aligns to 105:230/358 of 1ebuD

query
sites
1ebuD

L

V

E

E

R

N

G

G

K

I

S

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

M

A

I

F

A

S

H

S

H

D

G

L

I

A

E

T

L

W

A

K

R

A

I

L

A

F

E

S

A

N

K

K

G

P

G

T

N

N

I

-

G

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

F

L

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

V

V

T

E

K

K

V

I

M

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

T

E

M

F

R

S

D

T

S

S

G

Q

R

A

D

N

-

D

-

V

-

K

F

F

S

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

S

R

F

D

D

D

I

L

D

N

G

G

T

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

A

G

S

R

L

I

A

S

active site: D218 (= D202), K222 (= K206)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K106)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
37% identity, 28% coverage: 95:214/428 of query aligns to 105:230/358 of 1ebfA

query
sites
1ebfA

L

V

E

E

R

N

G

G

K

I

S

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

M

A

I

F

A

S

H

S

H

D

G

L

I

A

E

T

L

W

A

K

R

A

I

L

A

F

E

S

A

N

K

K

G

P

G

T

N

N

I

-

G

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

F

L

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

V

V

T

E

K

K

V

I

M

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

T

E

M

F

R

S

D

T

S

S

G

Q

R

A

D

N

-

D

-

V

-

K

F

F

S

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

S

R

F

D

D

D

I

L

D

N

G

G

T

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

A

G

S

R

L

I

A

S

active site: D218 (= D202), K222 (= K206)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A104)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

P31116 Homoserine dehydrogenase; HDH; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 28% coverage: 95:214/428 of query aligns to 106:231/359 of P31116

query
sites
P31116

L

V

E

E

R

N

G

G

K

I

S

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

M

A

I

F

A

S

H

S

H

D

G

L

I

A

E

T

L

W

A

K

R

A

I

L

A

F

E

S

A

N

K

K

G

P

G

T

N

N

I

-

G

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

F

L

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

V

V

T

E

K

K

V

I

M

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

T

E

M

F

R

S

D

T

S

S

G

Q

R

A

D

N

-

D

-

V

-

K

F

F

S

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

S

R

F

D

D

D

I

L

D

N

G

G

T

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

A

G

S

R

L

I

A

S

K117 (= K106) binding
E208 (= E191) binding ; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D202) mutation to L: Reduces kcat 150-fold.
K223 (= K206) mutation to V: Loss of activity.

Sites not aligning to the query:

11:18 binding
93 binding

O94671 Probable homoserine dehydrogenase; HDH; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 28% coverage: 95:214/428 of query aligns to 112:239/376 of O94671

query
sites
O94671

L

L

E

S

R

K

G

K

K

I

S

N

V

I

V

A

T

T

A

P

N

N

K

K

-

K

A

A

M

F

I

S

A

A

H

S

H

N

G

D

I

V

-

Y

-

Q

E

N

L

I

A

I

R

K

I

A

A

S

E

K

A

E

K

S

G

G

G

A

N

L

I

L

G

M

F

H

E

E

A

A

S

S

V

V

G

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

S

T

L

L

R

K

E

E

G

L

L

I

A

A

-

T

G

G

N

D

R

E

V

I

T

I

K

K

V

I

M

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

T

V

M

W

R

S

D

P

S

N

G

G

R

K

-

K

-

G

-

T

-

A

D
x
S

F

F

S

S

D

D

V

I

L

V

A

K

E

I

A

A

Q

K

A

Q

L

N

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

S

R

F

D

D

D

I

L

D

N

G

G

T

M

D

D

T

V

A

A

H

R

K

K

L

V

A

T

I

I

L

L

A

S

S

R

L

I

A

A

S201 (≠ D176) modified: Phosphoserine

Query Sequence

>WP_011386084.1 NCBI__GCF_000009985.1:WP_011386084.1
MSKAPLKIAIAGLGTVGGGTVQLLHDHAPLLAARAGRPLALVGAAALAKPADLPLDGVPF
FTDARDMAKTCDYDVLVELIGGAKGIALDVVRTALERGKSVVTANKAMIAHHGIELARIA
EAKGGNIGFEASVGGGIPVIKSLREGLAGNRVTKVMGILNGTCNYILTTMRDSGRDFSDV
LAEAQALGYAEADPSFDIDGTDTAHKLAILASLAFAMPLDIDAVSCEGIRNVSALDIRYA
DELGYRIKLLGVASLSDQGVETRVYPAMVPLSFPLAHVSGPFNAIVTEGDFVGRTVLEGR
GAGARPTASAVVADLMDLATGRVGPTFGIAVDALKALPAAPSGTHRSAYYIRLMVRDEPG
VFADVAGILKAHKVSMEQIIQRGRSPSEVVPVVMTVHETDEASMLGAVKAIRALGSVTDL
QLIRIESL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory