SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011386456.1 NCBI__GCF_000009985.1:WP_011386456.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4isdA Crystal structure of glutathione transferase homolog from burkholderia gl bgr1, target efi-501803, with bound glutathione
29% identity, 99% coverage: 3:207/208 of query aligns to 5:187/187 of 4isdA

query
sites
4isdA

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binding glutathione: Q43 (≠ I46), R51 (≠ K54), V52 (≠ I55), E64 (≠ D67), S65 (= S68)

4jbbA Crystal structure of glutathione s-transferase a6tby7(target efi- 507184) from klebsiella pneumoniae mgh 78578, gsh complex
27% identity, 82% coverage: 3:173/208 of query aligns to 4:176/208 of 4jbbA

query
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4jbbA

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active site: Y51 (≠ W49), A52 (≠ S50)
binding glutathione: S14 (= S13), Y16 (≠ W15), H43 (vs. gap), R56 (≠ K54), V57 (≠ I55), E69 (≠ D67), S70 (= S68)

P77544 Glutathione S-transferase YfcF; EC 2.5.1.18 from Escherichia coli (strain K12) (see paper)
30% identity, 83% coverage: 2:173/208 of query aligns to 5:178/214 of P77544

query
sites
P77544

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S10 (≠ V7) mutation to A: Nearly no effect on GSH transferase activity.
S16 (= S13) mutation to G: 2% of wild-type GSH transferase activity.

O43708 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Homo sapiens (Human) (see 10 papers)
36% identity, 48% coverage: 13:111/208 of query aligns to 14:115/216 of O43708

query
sites
O43708

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K32 (≠ R31) to E: in allele GSTZ1*C; dbSNP:rs7975
R42 (= R38) to G: in allele GSTZ1*B and allele GSTZ1*C; dbSNP:rs7972
Q45 (≠ E41) binding
V59 (≠ I55) binding
M82 (≠ R78) to T: in dbSNP:rs1046428
V99 (≠ A95) to M: in MAAID; decreased maleylacetoacetate isomerase activity
Q111 (≠ V107) binding

Sites not aligning to the query:

133 N → H: in dbSNP:rs2234955
150 A → V: in MAAID; decreased maleylacetoacetate isomerase activity

Q9WVL0 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Mus musculus (Mouse)
36% identity, 48% coverage: 13:111/208 of query aligns to 14:115/216 of Q9WVL0

query
sites
Q9WVL0

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Q45 (≠ A44) binding
V59 (≠ I55) binding
Q111 (≠ V107) binding

2cz2A Crystal structure of glutathione transferase zeta 1-1 (maleylacetoacetate isomerase) from mus musculus (form-1 crystal)
36% identity, 48% coverage: 13:111/208 of query aligns to 11:112/212 of 2cz2A

query
sites
2cz2A

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binding glutathione: S11 (= S13), C13 (≠ W15), L35 (= L37), Q42 (≠ A44), Q55 (≠ K54), V56 (≠ I55), Q68 (≠ D67), S69 (= S68), Q108 (≠ V107), N112 (≠ S111)

Sites not aligning to the query:

binding glutathione: 114

1fw1A Glutathione transferase zeta/maleylacetoacetate isomerase (see paper)
35% identity, 48% coverage: 13:111/208 of query aligns to 10:111/208 of 1fw1A

query
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1fw1A

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binding glutathione: S10 (= S13), C12 (≠ W15), L34 (= L37), Q41 (≠ E41), Q54 (≠ K54), V55 (≠ I55), Q67 (≠ D67), S68 (= S68), N111 (≠ S111)
binding sulfate ion: S11 (= S14), Q107 (≠ V107), Q110 (≠ R110)

Sites not aligning to the query:

3bbyA Crystal structure of glutathione s-transferase (np_416804.1) from escherichia coli k12 at 1.85 a resolution
29% identity, 83% coverage: 2:173/208 of query aligns to 3:158/191 of 3bbyA

query
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3bbyA

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P

Y

N

L

L

F

F

G

G

A

E

F

W

T

C

N

I

A

A

D

D

A

T

M

D

Y

L

A

A

P

L

V

M

V

I

T

N

R

R

I

L

binding calcium ion: D75 (= D88), E77 (= E90)

3qawA Crystal structure of a glutathione-s-transferase from antarctic clam laternula elliptica in a complex with glutathione (see paper)
26% identity, 89% coverage: 12:197/208 of query aligns to 7:198/219 of 3qawA

query
sites
3qawA

W

W

S

G

S

S

W

G

S
|
S

L

P

R
x
P

A

C

W

W

M

K

A

V

L

L

A

L

V

V

T

L

G

Q

A

E

P

K

-

K

-

I

-

D

F

Y

R

D

Q

E

V

K

F

I

I

I

E

S

L
x
F

R

S

Q

K

P

K

E

E

T
x
H

K
|
K

A

S

R

E

-

E

I

I

L

L

E

E

W

L

S

N

P

P

S

R

G

G

K
x
Q

I
x
V

P

P

M

T

L

F

D

T

D

D

D

G

G

D

I

V

K

V

V

V

W

N

D
x
E

S
|
S

L

T

A

A

I

I

C

C

E

M

Y

Y

L

L

A

E

E

E

R

K

F

Y

P

P

G

K

A

V

K

P

L

L

W

F

P

P

D

S

D

D

R

T

E

T

A

I

R

R

A

A

L

K

A

V

R

Y

S

Q

V

R

S

M

A

F

E

E

M

T

H

S

A

N

G

I

F

S

V

T

P

N

L

V

R

M

S

E

T

F

C

V

P

Q

M

Y

D

K

V

M

V

K

E

N

D

K

H

D

P

S

M

I

A

D

E

Q

I

V

P

L

D

L

D

K

V

E

K

K

A

K

D

D

V

K

A

A

R

H

V

V

D

E

A

L

L

G

W

H

T

W

E

E

C

N

R

Y

T

L

R

K

F

-

G

Q

Q

T

G

G

P

F

Y

V

L

A

F

T

G

K

A

E

F

F

T

T

N

M

A

A

D

D

A

V

M

F

Y

F

A

F

P

P

V

M

V

V

T

A

R

L

I

I

-

V

-

R

R

Q

T

G

Y

A

N

N

L

L

P

K

V

D

G

S

-

Y

P

P

V

N

A

I

G

F

A

K

Y

Y

C

Y

D

N

A

M

I

M

M

M

A

D

H

R

P

P

A

T

L

I

active site: S11 (= S16)
binding glutathione: S11 (= S16), P13 (≠ R18), F35 (≠ L37), H40 (≠ T42), K41 (= K43), Q53 (≠ K54), V54 (≠ I55), E66 (≠ D67), S67 (= S68)

1f2eA Structure of sphingomonad, glutathione s-transferase complexed with glutathione
31% identity, 88% coverage: 16:199/208 of query aligns to 11:193/201 of 1f2eA

query
sites
1f2eA

S

S

L

L

R

A

A

P

W

H

M

I

A

A

L

L

A

R

V

E

T

T

G

G

A

A

P

D

F

F

R

E

Q

A

V

V

F

K

I

V

E

D

L

L

-

A

-

V

R

R

Q

K

P

T

E

E

T

A

K

G

A

E

R

D

I

F

L

L

E

T

W

V

S

N

P

P

S

S

G

G

K
|
K

I
x
V

P

P

M

A

L

L

D

T

-

L

D

D

D

S

G

G

I

E

K

T

V

L

W

T

D
x
E

S
x
N

L

P

A

A

I

I

C

L

E

L

Y

Y

L

I

A

A

E

D

R

Q

F

N

P

P

G

A

A

S

K

G

L

L

W

A

P

P

D

A

D

E

R

G

E

S

A

L

-

D

-

R

-

Y

R

R

A

L

L

L

A

S

R

R

-

L

-

S

S

F

V

L

S

G

A

S

E

E

M

F

H
|
H

A

K

G

A

F

F

V

V

P

P

L

L

R

F

S

A

T

P

C

A

P

-

M

-

D

-

V

-

V

T

E

S

D

D

H

E

P

A

M

K

A

A

E

A

I

A

P

A

D

E

D

S

V

V

K

K

A

N

D

H

V

L

A

A

R

A

V

L

D

D

A

-

L

-

W

-

T

K

E

E

C

L

R

A

T

G

R

R

F

D

G

H

Q

Y

G

A

G

G

P

-

Y

-

L

-

F

-

G

N

A

A

F

F

T

S

N

V

A

A

D

D

A

-

M

I

Y

Y

A

L

P

Y

V

V

V

M

T

L

R

G

I

W

R

P

T

A

Y

Y

-

V

-

G

-

I

N

D

L

M

P

A

V

A

G

Y

P

P

V

A

A

L

G

G

A

A

Y

Y

C

A

D

G

A

K

I

I

M

A

A

Q

H

R

P

P

A

A

L

V

Q

G

A

A

binding glutathione: K51 (= K54), V52 (≠ I55), E65 (≠ D67), N66 (≠ S68), H106 (= H103)

Sites not aligning to the query:

binding glutathione: 10

6m1tA Bacterial beta class sphingomonas chungbukensis glutathione s- transferase
38% identity, 45% coverage: 16:109/208 of query aligns to 11:112/201 of 6m1tA

query
sites
6m1tA

S

S

L

L

R

A

A

P

W

H

M

I

A

A

L

L

A

R

V

E

T

T

G

G

A

A

P

A

F

F

R

D

Q

A

V

V

F

K

I

V

E

D

L

L

-

A

-

T

R

R

Q

K

P

V

E

E

T

T

K

G

A

D

R

D

I

F

L

L

E

T

W

V

S

N

P

P

S

S

G

G

K
|
K

I
x
V

P

P

M

A

L

L

D

T

-

L

D

D

D

S

G

G

I

E

K

T

V

L

W

T

D
x
E

S
x
N

L

P

A

A

I

I

C

L

E

L

Y

Y

L

I

A

A

E

D

R

Q

F

K

P

P

G

D

A

A

K

A

L

L

W

A

P

P

D

R

D

D

-

G

-

T

-

L

R

E

E

R

A

Y

R

R

A

L

L

I

A

S

R

R

-

L

-

S

S

F

V

L

S

G

A

S

E

E

M

F

H
|
H

A

K

G

A

F

F

V

V

P

P

L

L

binding glutathione: K51 (= K54), V52 (≠ I55), E65 (≠ D67), N66 (≠ S68), H106 (= H103)

Sites not aligning to the query:

Q64471 Glutathione S-transferase theta-1; GST class-theta-1; EC 2.5.1.18 from Mus musculus (Mouse) (see paper)
27% identity, 84% coverage: 26:200/208 of query aligns to 24:194/240 of Q64471

query
sites
Q64471

T

N

G

N

A

I

P

P

F

F

R

Q

Q

M

V

H

F

T

I

V

E

E

L

L

R

R

Q

K

P

G

E

E

T

H

K

L

A

S

R

D

I

A

L

F

-

A

E

R

W

V

S

N

P

P

S

M

G

K

K

R

I

V

P

P

M

A

L

M

D

M

D

D

D

G

G

G

I

F

K

T

V

L

W

C

D

E

S

S

L

V

A

A

I

I

C

L

E

L

Y

Y

L

L

A

A

E

H

R

K

F

Y

P

K

G

V

A

P

K

D

L

H

W

W

-

Y

P

P

D

Q

D

D

R

L

E

Q

A

A

R

R

A

A

L

R

A

V

R

D

S

E

V

Y

S

L

A

A

E

W

M

Q

H

H

A

T

G

G

F

-

V

-

P

-

L

L

R

R

S

R

T

S

C

C

-

L

-

R

-

A

-

L

-

W

-

H

-

K

-

V

-

M

-

F

P

P

M

V

D

F

V

L

V

G

E

E

D

Q

H

I

P

P

M

-

A

-

E

-

I

-

P

P

D

E

D

T

V

L

K

A

A

A

D

T

V

L

A

A

R

E

V

L

D

D

A

V

L

N

W

L

T

Q

E

V

C

L

R

E

T

D

R

K

F

F

G

L

Q

Q

G

D

G

K

P

D

Y

F

L

L

F

V

G

G

A

P

F

H

T

I

N

S

A

L

D

A

A

D

M

L

Y

V

A

A

P

-

V

-

V

I

T

T

R

E

I

L

R

-

T

-

Y

-

N

-

L

-

P

-

V

M

G

H

P

P

V

V

A

G

G

G

A

G

Y

-

C

C

D

P

A

V

I

F

M

E

A

G

H

H

P

P

A

R

L

L

Q

A

A

A

W

W

Sites not aligning to the query:

234 R→W: Strongly reduced catalytic activity.

4pxoA Crystal structure of maleylacetoacetate isomerase from methylobacteriu extorquens am1 with bound malonate and gsh (target efi-507068)
32% identity, 51% coverage: 10:116/208 of query aligns to 4:120/216 of 4pxoA

query
sites
4pxoA

K

K

R

M

W

Y

S

G

S

N

W

W

-

R

-
x
S

-

A

-
x
A

S

A

L

F

R

R

A

V

W

R

M

I

A

A

L

L

A

N

V

L

T

K

G

G

A

I

P

A

F

Y

R

E

Q

E

V

V

F

F

I

L

E

D

L

L

R

D

Q

A

-

G

P

D

E
x
Q

T

H

K

K

A

P

R

D

I

F

L

L

E

A

W

I

S

N

P

P

S

Q

G

G

K
x
A

I
x
V

P

P

-

A

M

L

L

F

D

D

D

G

D

D

G

G

I

P

K

P

V

L

W

T

D
x
Q

S
|
S

L

L

A

A

I

I

C

L

E

D

Y

Y

L

L

A

E

E

E

R

T

F

R

P

T

G

G

A

V

K

P

L

L

W

L

P

P

D

E

-

P

-

R

-

A

-

R

R

A

E

R

A

A

R

R

A

S

L

L

A

A

R

Q

S

V

V

V

S

A

A

C

E

D

M

T

H
|
H

A

P

G

L

F

Y

V

V

P
|
P

L
x
R

R

V

S

R

T

T

C

F

P

L

M

M

D

E

binding glutathione: S11 (vs. gap), A13 (vs. gap), Q40 (≠ E41), A53 (≠ K54), V54 (≠ I55), Q67 (≠ D67), S68 (= S68), H107 (= H103), P112 (= P108), R113 (≠ L109)

3vlnA Human glutathione transferase o1-1 c32s mutant in complex with ascorbic acid (see paper)
24% identity, 72% coverage: 4:152/208 of query aligns to 21:166/239 of 3vlnA

query
sites
3vlnA

T

S

L

I

V

R

V

I

G

Y

T

S

K

M

R

R

W

F

S

S

S

P

W
x
F

S

A

L

E

R

R

A

T

W

R

M

L

A

V

L

L

A

K

V

A

T

K

G

G

A

I

P

R

F

H

R

E

Q

V

V

I

F

N

I

I

E

N

L

L

R

K

-

N

Q

K

P

P

E

E

T

W

K

-

A

-

R

-

I

F

L

F

E

K

W

K

S

N

P

P

S

F

G

G

K

L

I
x
V

P
|
P

M

V

L

L

D

E

D

N

-

S

D

Q

G

G

I

Q

K

L

V

I

W

Y

D

E

S
|
S

L

A

A

I

I

T

C

C

E

E

Y

Y

L

L

A

D

E

E

R

A

F

Y

P

P

G

G

A

K

K

K

L

L

W

L

P

P

D

D

R

P

E

Y

A

E

R

K

A

A

L

C

A

Q

R

K

S

M

V

I

S

-

A

L

E

E

M

L

H

F

A

S

G

K

F

-

V

V

P

P

L

S

R

L

S

V

T

G

C

S

P

F

M

I

D

R

V

S

V

Q

E

N

D

K

H

E

P

D

M

Y

A

A

E

G

I

L

P

K

D

E

V

F

K

R

A

K

D

E

V

F

A

T

R

K

V

L

D

E

A

E

L

V

W

L

T

T

E

N

C

K

R

K

T

T

R

T

F

F

G

F

Q

G

G

G

G

N

binding ascorbic acid: F32 (≠ W15), V70 (≠ I55), P71 (= P56), S84 (= S68)

6mhbA Glutathione s-transferase omega 1 bound to covalent inhibitor 18 (see paper)
23% identity, 72% coverage: 4:152/208 of query aligns to 18:160/233 of 6mhbA

query
sites
6mhbA

T

S

L

I

V

R

V

I

G

Y

T

S

K

M

R

R

W

F

S
x
C

S
x
P

W

F

S

A

L

E

R

R

A

T

W

R

M

L

A

V

L

L

A

K

V

A

T

K

G

G

A

I

P

R

F

H

R

E

Q

V

V

I

F

N

I

I

E

N

L

L

R

K

-

N

Q

K

P

P

E

E

T

W

K

-

A

-

R

-

I

F

L

F

E

K

W

K

S

N

P

P

S

F

G

G

K

L

I

V

P

P

M

V

L

L

D

E

D

N

-

S

D

Q

G

G

I

Q

K

L

V

I

W

Y

D

E

S

S

L

A

A

I

I

T

C

C

E

E

Y

Y

L

L

A

D

E

E

R

A

F

Y

P

P

G

G

A

K

K

K

L

L

W

L

P

P

D

D

R

P

E

Y

A

E

R

K

A

A

L

C

A

Q

R

K

S

M

V

I

S

-

A

-

E

-

M

-

H

-

A

-

G

-

F

-

V

L

P

E

L

L

R

F

S

S

T

K

C

V

P

P

M

S

D

L

V

V

-

G

-

S

-

F

V

I

E

R

D

K

H

E

P

D

M

Y

A

A

E

G

I

L

P

K

D

E

V

F

K

R

A

K

D

E

V

F

A

T

R

K

V

L

D

E

A

E

L

V

W

L

T

T

E

N

C

K

R

K

T

T

R

T

F

F

G

F

Q

G

G

G

G

N

binding N-[4-(4-chlorophenyl)-1,3-thiazol-2-yl]propanamide: C27 (≠ S13), P28 (≠ S14)

Sites not aligning to the query:

binding N-[4-(4-chlorophenyl)-1,3-thiazol-2-yl]propanamide: 175, 214, 217, 218

4yquA Glutathione s-transferase omega 1 bound to covalent inhibitor c1-31 (see paper)
23% identity, 72% coverage: 4:152/208 of query aligns to 20:162/235 of 4yquA

query
sites
4yquA

T

S

L

I

V

R

V

I

G

Y

T

S

K

M

R

R

W

F

S
x
C

S
x
P

W
x
F

S

A

L

E

R

R

A

T

W

R

M

L

A

V

L

L

A

K

V

A

T

K

G

G

A

I

P

R

F

H

R

E

Q

V

V

I

F

N

I

I

E

N

L

L

R

K

-

N

Q

K

P

P

E

E

T

W

K

-

A

-

R

-

I

F

L

F

E

K

W

K

S

N

P

P

S

F

G

G

K

L

I

V

P

P

M

V

L

L

D

E

D

N

-

S

D

Q

G

G

I

Q

K

L

V

I

W

Y

D

E

S

S

L

A

A

I

I

T

C

C

E

E

Y

Y

L

L

A

D

E

E

R

A

F

Y

P

P

G

G

A

K

K

K

L

L

W

L

P

P

D

D

R

P

E

Y

A

E

R

K

A

A

L

C

A

Q

R

K

S

M

V

I

S

-

A

-

E

-

M

-

H

-

A

-

G

-

F

-

V

L

P

E

L

L

R

F

S

S

T

K

C

V

P

P

M

S

D

L

V

V

-

G

-

S

-

F

V
x
I

E
x
R

D

K

H

E

P

D

M

Y

A

A

E

G

I

L

P

K

D

E

V

F

K

R

A

K

D

E

V

F

A

T

R

K

V

L

D

E

A

E

L

V

W

L

T

T

E

N

C

K

R

K

T

T

R

T

F

F

G

F

Q

G

G

G

G

N

binding N-{5-(azepan-1-ylsulfonyl)-2-[(ethylsulfanyl)methoxy]phenyl}acetamide: C29 (≠ S13), P30 (≠ S14), F31 (≠ W15), I128 (≠ V118), R129 (≠ E119)

Sites not aligning to the query:

binding N-{5-(azepan-1-ylsulfonyl)-2-[(ethylsulfanyl)methoxy]phenyl}acetamide: 223

1jlvA Anopheles dirus species b glutathione s-transferases 1-3 (see paper)
41% identity, 26% coverage: 43:97/208 of query aligns to 40:95/207 of 1jlvA

query
sites
1jlvA

K

K

A

P

R

E

I

F

L

L

E

K

W

I

S

N

P

P

S

Q

G
x
H

K
x
C

I
|
I

P

P

M

T

L

L

D

V

D

D

D

N

G

G

I

F

K

A

V

L

W

W

D
x
E

S
|
S

L

R

A

A

I

I

C

C

E

T

Y

Y

L

L

A

A

E

E

R

K

F

Y

-

G

P

K

G

D

A

D

K

K

L

L

W

Y

P

P

D

K

D

D

R

P

E

Q

A

K

R

R

A

A

L

V

A

V

R

N

S

Q

binding glutathione: H50 (≠ G53), C51 (≠ K54), I52 (= I55), E64 (≠ D67), S65 (= S68)

Sites not aligning to the query:

active site: 9
binding glutathione: 11, 101

4is0A Structural insights into omega-class glutathione transferases: a snapshot of enzyme reduction and identification of the non-catalytic ligandin site. (see paper)
23% identity, 72% coverage: 4:152/208 of query aligns to 20:165/238 of 4is0A

query
sites
4is0A

T

S

L

I

V

R

V

I

G

Y

T

S

K

M

R

R

W

F

S
x
A

S

P

W
x
F

S

A

L

E

R
|
R

A

T

W

R

M

L

A

V

L

L

A

K

V

A

T

K

G

G

A

I

P

R

F

H

R

E

Q

V

V

I

F

N

I

I

E

N

L
|
L

R
x
K

-

N

Q
x
K

P

P

E

E

T

W

K

-

A

-

R

-

I

F

L

F

E

K

W

K

S

N

P

P

S

F

G

G

K
x
L

I
x
V

P

P

M

V

L

L

D

E

D

N

-

S

D

Q

G

G

I

Q

K

L

V

I

W

Y

D
x
E

S
|
S

L
x
A

A

I

I

T

C

C

E
|
E

Y

Y

L

L

A

D

E

E

R

A

F

Y

P

P

G

G

A

K

K

K

L

L

W

L

P

P

D

D

R

P

E

Y

A

E

R

K

A

A

L

C

A

Q

R
x
K

S

M

V

I

S

-

A
x
L

E

E

M

L

H

F

A

S

G

K

F

-

V

V

P

P

L

S

R

L

S

V

T

G

C

S

P

F

M

I

D

R

V

S

V

Q

E

N

D

K

H

E

P

D

M

Y

A

A

E

G

I

L

P

K

D

E

V

F

K

R

A

K

D

E

V

F

A

T

R

K

V

L

D

E

A

E

L

V

W

L

T

T

E

N

C

K

R

K

T

T

R

T

F

F

G

F

Q

G

G

G

G

N

binding L-gamma-glutamyl-S-[2-(4-nitrophenyl)-2-oxoethyl]-L-cysteinylglycine: R34 (= R18), A84 (≠ L69), E88 (= E73), K111 (≠ R96), L114 (≠ A100)
binding oxidized glutathione disulfide: A29 (≠ S13), F31 (≠ W15), L53 (= L37), K54 (≠ R38), K56 (≠ Q39), L68 (≠ K54), V69 (≠ I55), E82 (≠ D67), S83 (= S68)

Sites not aligning to the query:

7rhpA Crystal structure of honeybee (apis mellifera) glutathione s- transferase amgstd1 (see paper)
33% identity, 37% coverage: 22:97/208 of query aligns to 21:102/215 of 7rhpA

query
sites
7rhpA

A

A

L

V

A

A

V

L

T

T

G

A

A

A

-

A

-

L

-

D

-

I

-

E

-

M

P

N

F

F

R

K

Q

Q

V

V

F

N

I

L

E

-

L

M

R

N

Q

G

P

E

E
x
H

T

L

K

K

A

P

R

E

I

F

L

L

E

K

W

I

S

N

P

P

S

Q

G
x
H

K
x
T

I
|
I

P

P

M

T

L

I

D

D

D

N

G

G

I

F

K

R

V

L

W

W

D
x
E

S
|
S

L

R

A

A

I

I

C

M

E

T

Y

Y

L

L

A

A

E

D

R

Q

F

Y

-

G

P

K

G

N

A

D

K

T

L

L

W

Y

P

P

D

K

D

D

R

L

E

K

A

K

R

R

A

A

L

I

A

V

R

N

S

Q

binding glutathione: H45 (≠ E41), H57 (≠ G53), T58 (≠ K54), I59 (= I55), E71 (≠ D67), S72 (= S68)

6mhdA Glutathione s-transferase omega 1 bound to covalent inhibitor 44 (see paper)
23% identity, 72% coverage: 4:152/208 of query aligns to 26:171/244 of 6mhdA

query
sites
6mhdA

T

S

L

I

V

R

V

I

G

Y

T

S

K

M

R

R

W

F

S
x
C

S

P

W

F

S

A

L

E

R

R

A

T

W

R

M

L

A

V

L

L

A

K

V

A

T

K

G

G

A

I

P

R

F

H

R

E

Q

V

V

I

F

N

I

I

E

N

L
|
L

R

K

-

N

Q

K

P

P

E

E

T

W

K

-

A

-

R

-

I

F

L

F

E

K

W

K

S

N

P

P

S

F

G

G

K

L

I

V

P

P

M

V

L

L

D

E

D

N

-

S

D

Q

G

G

I

Q

K

L

V

I

W

Y

D

E

S

S

L

A

A

I

I

T

C

C

E

E

Y

Y

L

L

A

D

E

E

R

A

F

Y

P

P

G

G

A

K

K

K

L

L

W

L

P

P

D

D

R

P

E

Y

A

E

R

K

A

A

L

C

A

Q

R

K

S

M

V

I

S

-

A

L

E

E

M

L

H

F

A

S

G

K

F

-

V

V

P

P

L

S

R

L

S

V

T
x
G

C

S

P

F

M
x
I

D

R

V

S

V

Q

E

N

D

K

H

E

P

D

M

Y

A

A

E

G

I

L

P

K

D

E

V

F

K

R

A

K

D

E

V

F

A

T

R

K

V

L

D

E

A

E

L

V

W

L

T

T

E

N

C

K

R

K

T

T

R

T

F

F

G

F

Q

G

G

G

G

N

binding N-[(3-methyl-1,2-oxazol-5-yl)methyl]-N-(4-phenyl-1,3-thiazol-2-yl)propanamide: C35 (≠ S13), L59 (= L37), G131 (≠ T112), I134 (≠ M115)

Sites not aligning to the query:

binding N-[(3-methyl-1,2-oxazol-5-yl)methyl]-N-(4-phenyl-1,3-thiazol-2-yl)propanamide: 232

Query Sequence

>WP_011386456.1 NCBI__GCF_000009985.1:WP_011386456.1
MSLTLVVGTKRWSSWSLRAWMALAVTGAPFRQVFIELRQPETKARILEWSPSGKIPMLDD
DGIKVWDSLAICEYLAERFPGAKLWPDDREARALARSVSAEMHAGFVPLRSTCPMDVVED
HPMAEIPDDVKADVARVDALWTECRTRFGQGGPYLFGAFTNADAMYAPVVTRIRTYNLPV
GPVAGAYCDAIMAHPALQAWVMEARIAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory