SitesBLAST

S109 (≠ E54) to W: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ T72) to K: in D2HGA1; unknown pathological significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (≠ A76) to V: in D2HGA1; unknown pathological significance; complete loss of catalytic activity
I147 (= I92) to S: in D2HGA1; severe phenotype; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ T98) to T: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; to V: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ V115) to Y: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (≠ G132) to L: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A148) to V: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (= A174) to V: in D2HGA1; unknown pathological significance; significant loss of catalytic activity
G233 (= G176) to S: in D2HGA1; unknown pathological significance; no effect on catalytic activity; dbSNP:rs374535734
D375 (= D369) to Y: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs267606759
R386 (= R380) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
T390 (≠ F384) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
V399 (= V390) to M: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs746519212
K401 (= K392) binding ; binding ; mutation to A: Loss of catalytic activity.
R419 (≠ E417) to H: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs199908032
A426 (≠ G424) to T: in D2HGA1; unknown pathological significance; no effect on catalytic activity; dbSNP:rs146578303
H434 (= H433) binding ; mutation to A: Loss of catalytic activity.
G436 (≠ L435) to V: slight reduction in catalytic activity
N439 (= N438) to D: in D2HGA1; mild phenotype; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs121434362
H441 (= H440) binding ; mutation to A: Loss of catalytic activity.
N443 (≠ T442) binding ; mutation to A: Significantly reduced catalytic activity.
V444 (≠ F443) to A: in D2HGA1; severe phenotype; unknown pathological significance; significant reduction in catalytic activity; dbSNP:rs121434360
A446 (≠ Q445) to V: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs746956176
E475 (= E478) binding ; mutation to A: Loss of catalytic activity.
H476 (= H479) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
G477 (= G480) to R: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; unknown pathological significance)
400:521 natural variant: Missing (in D2HGA1; unknown pathological significance; complete loss of catalytic activity)

6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
25% identity, 52% coverage: 28:516/936 of query aligns to 1:461/467 of 6lpnB

query
sites
6lpnB

P

P

L

V

R

R

L

L

A

P

Y

F

G

S

T

T

D

V

-

S

-

K

-

Q

-

D

-

L

A

A

S

A

F

F

Y

E

R

R

L

I

V

V

P

P

-

G

-

V

-

T

-

D

-

P

-

E

-

A

-

L

-

Q

-

A

-

P

-

N

-

V

-

D

-
x
W

-

L

-

R

-

T

-

L

-

R

-

G

-

C

-

S

Q

K

V

V

V

L

A

L

E

R

V

P

R

R

D

T

E

S

A

E

E

E

V

V

K

S

G

H

V

I

L

L

A

R

A

H

C

C

R

H

R

E

H

R

G

N

A

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

D

V

S

F

V

D

L

E

M

I

I

I

L

L

G

S

T

T

G

A

W

R

T

M

Q

N

A

R

V

V

V

L

E

S

D

F

E

H

G

S

K

V

R

S

-

G

-

I

I

L

R

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

R

Y

L

V

A

E

F

R

A

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

D

G

S

S

C
|
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
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A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

S

Y

D

G

N

S

S

L

Y

H

Q

G

T

T

V

V

M

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

V

L

D

D

T

C

G

-

N

-

P

-

E

-

S

-

V

L

A

T

A

S

F

L

R

R

A

-

S

-

H

-

A

-

E

-

L

-

S

-

R

-

L

-

D

-

M

-

G

-

R

-

V

-

R

-

D

-

E

-

T

-

L

-

A

-

G

-

R

-

I

-

R

-

H

-

K

-

F

-

A

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

V

-

D

-

F

-

T

-

D

-

P

-

L

-

D

-

I

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L

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-

H

Q

L

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M

F

I

I

G

G

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S

E
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E

G
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G

T

T

L

L

G

G

F
x
I

I
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I

A

T

E

T

I

V

T

S

Y

I

R

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

S

V

A

A

L

F

L

L

L

G

F

C

P

P

D

G

I

F

A

A

E

E

A

V

C

L

R

Q

A

T

V

F

V

S

A

T

L

C

K

K

Q

G

A

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

V

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C

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M

R

Q

C

L

V

V

E

G

D

R

K

H

P

L

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H

M

L

P

A

A

S

Q

P

I

V

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Q

G

-

L

-

A

-

D

E

G

S

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P

T

F

S

Y

L

V

L

L

V

I

E

E

A

T

R

S

G

G

E

S

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N

A

A

E

G

A

H

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A

A

A

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N

K

L

L

A

G

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I

F

G

L

R

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V

H

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A

S

L

G

G

V

S

T

G

T

L

L

V

F

T

P

D

P

G

A

T

F

M

T

A

D

T

D

D

P

Q

Y

R

E

K

Y

V

G

K

T

M

L

L

W

W

K

A

I

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R

R

K

E

G

R

L

I

F
x
T

P

E

A

A

L

L

-

S

-

R

-

D

G

G

A

Y

V

V

R

Y

K

K

V

Y

G

-

T

-

V

-

I

-

E

-

D

D

V

L

A

S

F

L

P

P

I

V

E

E

S

R

L

L

A

Y

A

D

A

I

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V

T

T

D

D

L

L

E

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A

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R

G

K

P

H

H

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A

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-

D

K

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A

V

I

V

I

G

F

Y

G

G

H
|
H

A

L

L

G

D

D

G

G

N

N

L

L

H

H

F

L

T

N

F

V

T

T

Q

A

D

E

F

-

G

-

I

-

K

A

E

F

E

S

V

P

D

S

R

L

Y

L

A

A

R

A

F

L

M

E

D

P

E

H

V

V

A

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E

E

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W

V

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V

A

N

G

K

Q

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
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E

H
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H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

G

M

Y

E

S

W

K

G

P

T

P

E

G

A

A

T

L

A

Q

L

L

M

M

W

Q

D

Q

I

L

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K

G

A

L

L

D

D

P

P

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K

G

G

L

I

L

L

N
|
N

P

P

binding flavin-adenine dinucleotide: W40 (vs. gap), P76 (≠ F73), Q77 (≠ R74), G78 (≠ A75), G79 (≠ A76), N80 (≠ G77), T81 (= T78), G82 (≠ S79), M83 (≠ L80), G86 (≠ Q83), S87 (≠ A84), L140 (≠ P135), G141 (≠ A136), A142 (≠ S137), C146 (= C141), H147 (≠ K142), G149 (= G144), G150 (= G145), N151 (≠ I146), A153 (= A148), T154 (≠ N149), A156 (= A151), G157 (≠ S152), G158 (= G153), E207 (= E253), G208 (= G254), I212 (≠ F258), I213 (= I259), T338 (≠ F384), H382 (= H433), E423 (= E478), H424 (= H479), N460 (= N515)

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
25% identity, 50% coverage: 46:516/936 of query aligns to 48:460/466 of 6lpxA

query
sites
6lpxA

Q

K

V

V

V

L

A

L

E

R

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P

R

R

D

T

E

S

A

E

E

E

V

V

K

S

G

H

V

I

L

L

A

R

A

H

C

C

R

H

R

E

H

R

G

N

A

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

D

V

S

F

V

D

L

E

M

I

I

I

L

L

G

S

T

T

G

A

W

R

T

M

Q

N

A

R

V

V

V

L

E

S

D

F

E

H

G

S

K

V

R

S

-

G

-

I

I

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V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

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R

R

Y

L

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A

E

F

R

A

D

R

F

K

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I

M

G

P

P

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D

D

P
x
L

A
x
G

S
x
A

I

K

D

G

S

S

C
|
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
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A

N
x
T

N

N

A
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A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

S

Y

D

G

N

S

S

L

Y

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Q

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M

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E

L

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V

V

L

L

A

A

D

D

G

G

T

T

L

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V

L

D

D

T

C

G

-

N

-

P

-

E

-

S

-

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L

A

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A

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F

L

R

R

A

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S

-

H

-

A

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E

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L

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S

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L

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D

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M

-

G

-

R

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D

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T

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L

-

A

-

G

-

R

-

I

-

R

-

H

-

K

-

F

-

A

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

V

-

D

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F

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T

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D

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P

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L

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D

-

I

-

L

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T

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H

Q

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M

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I

I

G

G

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E

G
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G

T

T

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L

G

G

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x
I

I
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I

A

T

E

T

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V

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P

P

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K

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G

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A

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R

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T

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E

A

A

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L

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K

V

Y

G

-

T

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V

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E

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D

D

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A

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D

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L

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A

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C

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R

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K

P

H

H

G

A

Y

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D

K

E

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I

V

I

G

F
x
Y

G

G

H
|
H

A

L

L

G

D

D

G

G

N

N

L

L

H
|
H

F

L

T
x
N

F

V

T

T

Q

A

D

E

F

-

G

-

I

-

K

A

E

F

E

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Y

L

A

A

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A

F

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M

E

D

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E

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A

Y

E

E

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W

V

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A

N

G

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Y

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D

Q

G

G

S

S

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K

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A

A

E
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E

H
|
H

G

G

T

V

G

G

R

F

N

R

M

K

A

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P

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F

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V

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E

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E

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W

K

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G

A

A

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A

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L

M

M

W

Q

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Q

I

L

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K

G

A

L

L

D

D

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P

L

K

G

G

L

I

L

L

N
|
N

P

P

binding 2-oxoglutaric acid: R333 (= R380), T337 (≠ F384), K348 (= K392), Y379 (≠ F431), H381 (= H433), H388 (= H440), N390 (≠ T442), H423 (= H479)
binding flavin-adenine dinucleotide: P75 (≠ F73), Q76 (≠ R74), G77 (≠ A75), G78 (≠ A76), N79 (≠ G77), T80 (= T78), G81 (≠ S79), M82 (≠ L80), G85 (≠ Q83), S86 (≠ A84), L139 (≠ P135), G140 (≠ A136), A141 (≠ S137), C145 (= C141), H146 (≠ K142), G148 (= G144), G149 (= G145), N150 (≠ I146), A152 (= A148), T153 (≠ N149), A155 (= A151), G156 (≠ S152), G157 (= G153), E206 (= E253), G207 (= G254), I211 (≠ F258), I212 (= I259), H381 (= H433), E422 (= E478), H423 (= H479), N459 (= N515)
binding zinc ion: H381 (= H433), H388 (= H440), E422 (= E478)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
25% identity, 50% coverage: 46:516/936 of query aligns to 48:460/466 of 6lpuA

query
sites
6lpuA

Q

K

V

V

V

L

A

L

E

R

V

P

R

R

D

T

E

S

A

E

E

E

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V

K

S

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H

V

I

L

L

A

R

A

H

C

C

R

H

R

E

H

R

G

N

A

L

P

A

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V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

D

V

S

F

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M

I

I

I

L

L

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T

G

A

W

R

T

M

Q

N

A

R

V

V

V

L

E

S

D

F

E

H

G

S

K

V

R

S

-

G

-

I

I

L

R

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

R

Y

L

V

A

E

F

R

A

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

D

G

S

S

C
|
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
|
A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

S

Y

D

G

N

S

S

L

Y

H

Q

G

T

T

V

V

M

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

V

L

D

D

T

C

G

-

N

-

P

-

E

-

S

-

V

L

A

T

A

S

F

L

R

R

A

-

S

-

H

-

A

-

E

-

L

-

S

-

R

-

L

-

D

-

M

-

G

-

R

-

V

-

R

-

D

-

E

-

T

-

L

-

A

-

G

-

R

-

I

-

R

-

H

-

K

-

F

-

A

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

V

-

D

-

F

-

T

-

D

-

P

-

L

-

D

-

I

-

L

-

T

-

H

Q

L

L

M

F

I

I

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F
x
I

I
|
I

A

T

E

T

I

V

T

S

Y

I

R

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

S

V

A

A

L

F

L

L

L

G

F

C

P

P

D

G

I

F

A

A

E

E

A

V

C

L

R

Q

A

T

V

F

V

S

A

T

L

C

K

K

Q

G

A

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

V

S

C

L

M

R

Q

C

L

V

V

E

G

D

R

K

H

P

L

G

H

M

L

P

A

A

S

Q

P

I

V

R

Q

G

-

L

-

A

-

D

E

G

S

V

P

T

F

S

Y

L

V

L

L

V

I

E

E

A

T

R

S

G

G

E

S

T

N

A

A

E

G

A

H

L

D

A

A

A

E

N

K

L

L

A

G

E

H

I

F

G

L

R

E

V

H

L

A

S

L

G

G

V

S

T

G

T

L

L

V

F

T

P

D

P

G

A

T

F

M

T

A

D

T

D

D

P

Q

Y

R

E

K

Y

V

G

K

T

M

L

L

W

W

K

A

I

L

R
|
R

K

E

G

R

L

I

F
x
T

P

E

A

A

L

L

-

S

-

R

-

D

G

G

A

Y

V

V

R

Y

K
|
K

V

Y

G

-

T

-

V

-

I

-

E

-

D

D

V

L

A

S

F

L

P

P

I

V

E

E

S

R

L

L

A

Y

A

D

A

I

T

V

T

T

D

D

L

L

E

R

H

A

L

R

C

L

R

G

K

P

H

H

G

A

Y

-

D

K

E

H

A

V

I

V

I

G

F
x
Y

G

G

H
|
H

A

L

L

G

D

D

G

G

N

N

L

L

H
|
H

F

L

T
x
N

F

V

T

T

Q

A

D

E

F

-

G

-

I

-

K

A

E

F

E

S

V

P

D

S

R

L

Y

L

A

A

R

A

F

L

M

E

D

P

E

H

V

V

A

Y

E

E

L

W

V

T

V

A

N

G

K

Q

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
|
E

H
|
H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

G

M

Y

E

S

W

K

G

P

T

P

E

G

A

A

T

L

A

Q

L

L

M

M

W

Q

D

Q

I

L

K

K

G

A

L

L

D

D

P

P

L

K

G

G

L

I

L

L

N
|
N

P

P

binding flavin-adenine dinucleotide: P75 (≠ F73), Q76 (≠ R74), G77 (≠ A75), G78 (≠ A76), N79 (≠ G77), T80 (= T78), G81 (≠ S79), M82 (≠ L80), G85 (≠ Q83), S86 (≠ A84), L139 (≠ P135), G140 (≠ A136), A141 (≠ S137), C145 (= C141), H146 (≠ K142), G148 (= G144), G149 (= G145), N150 (≠ I146), A152 (= A148), T153 (≠ N149), A155 (= A151), G156 (≠ S152), G157 (= G153), E206 (= E253), G207 (= G254), I211 (≠ F258), I212 (= I259), H381 (= H433), E422 (= E478), H423 (= H479), N459 (= N515)
binding (2s)-2-hydroxypentanedioic acid: M82 (≠ L80), R333 (= R380), T337 (≠ F384), K348 (= K392), Y379 (≠ F431), H381 (= H433), H388 (= H440), N390 (≠ T442), H423 (= H479)
binding zinc ion: H381 (= H433), H388 (= H440), E422 (= E478)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39

6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
25% identity, 50% coverage: 46:516/936 of query aligns to 48:460/466 of 6lpqA

query
sites
6lpqA

Q

K

V

V

V

L

A

L

E

R

V

P

R

R

D

T

E

S

A

E

E

E

V

V

K

S

G

H

V

I

L

L

A

R

A

H

C

C

R

H

R

E

H

R

G

N

A

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

D

V

S

F

V

D

L

E

M

I

I

I

L

L

G

S

T

T

G

A

W

R

T

M

Q

N

A

R

V

V

V

L

E

S

D

F

E

H

G

S

K

V

R

S

-

G

-

I

I

L

R

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

R

Y

L

V

A

E

F

R

A

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

D

G

S

S

C
|
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
|
A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

S

Y

D

G

N

S

S

L

Y

H

Q

G

T

T

V

V

M

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

V

L

D

D

T

C

G

-

N

-

P

-

E

-

S

-

V

L

A

T

A

S

F

L

R

R

A

-

S

-

H

-

A

-

E

-

L

-

S

-

R

-

L

-

D

-

M

-

G

-

R

-

V

-

R

-

D

-

E

-

T

-

L

-

A

-

G

-

R

-

I

-

R

-

H

-

K

-

F

-

A

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

V

-

D

-

F

-

T

-

D

-

P

-

L

-

D

-

I

-

L

-

T

-

H

Q

L

L

M

F

I

I

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G
|
G

F
x
I

I
|
I

A

T

E

T

I

V

T

S

Y

I

R

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

S

V

A

A

L

F

L

L

L

G

F

C

P

P

D

G

I

F

A

A

E

E

A

V

C

L

R

Q

A

T

V

F

V

S

A

T

L

C

K

K

Q

G

A

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

V

S

C

L

M

R

Q

C

L

V

V

E

G

D

R

K

H

P

L

G

H

M

L

P

A

A

S

Q

P

I

V

R

Q

G

-

L

-

A

-

D

E

G

S

V

P

T

F

S

Y

L

V

L

L

V

I

E

E

A

T

R

S

G

G

E

S

T

N

A

A

E

G

A

H

L

D

A

A

A

E

N

K

L

L

A

G

E

H

I

F

G

L

R

E

V

H

L

A

S

L

G

G

V

S

T

G

T

L

L

V

F

T

P

D

P

G

A

T

F

M

T

A

D

T

D

D

P

Q

Y

R

E

K

Y

V

G

K

T

M

L

L

W

W

K

A

I

L

R
|
R

K

E

G

R

L

I

F
x
T

P

E

A

A

L

L

-

S

-

R

-

D

G

G

A

Y

V

V

R

Y

K
|
K

V

Y

G

-

T

-

V

-

I

-

E

-

D

D

V

L

A

S

F

L

P

P

I

V

E

E

S

R

L

L

A

Y

A

D

A

I

T

V

T

T

D

D

L

L

E

R

H

A

L

R

C

L

R

G

K

P

H

H

G

A

Y

-

D

K

E

H

A

V

I

V

I

G

F
x
Y

G

G

H
|
H

A

L

L

G

D

D

G

G

N

N

L

L

H
|
H

F

L

T

N

F

V

T

T

Q

A

D

E

F

-

G

-

I

-

K

A

E

F

E

S

V

P

D

S

R

L

Y

L

A

A

R

A

F

L

M

E

D

P

E

H

V

V

A

Y

E

E

L

W

V

T

V

A

N

G

K

Q

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
|
E

H
|
H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

G

M

Y

E

S

W

K

G

P

T

P

E

G

A

A

T

L

A

Q

L

L

M

M

W

Q

D

Q

I

L

K

K

G

A

L

L

D

D

P

P

L

K

G

G

L

I

L

L

N
|
N

P

P

binding flavin-adenine dinucleotide: P75 (≠ F73), Q76 (≠ R74), G77 (≠ A75), G78 (≠ A76), N79 (≠ G77), T80 (= T78), G81 (≠ S79), G85 (≠ Q83), S86 (≠ A84), L139 (≠ P135), G140 (≠ A136), A141 (≠ S137), C145 (= C141), H146 (≠ K142), G148 (= G144), G149 (= G145), N150 (≠ I146), A152 (= A148), T153 (≠ N149), A155 (= A151), G156 (≠ S152), G157 (= G153), E206 (= E253), G207 (= G254), G210 (= G257), I211 (≠ F258), I212 (= I259), H381 (= H433), E422 (= E478), H423 (= H479), N459 (= N515)
binding d-malate: M82 (≠ L80), R333 (= R380), T337 (≠ F384), K348 (= K392), Y379 (≠ F431), H381 (= H433), H388 (= H440), E422 (= E478), H423 (= H479)
binding zinc ion: H381 (= H433), H388 (= H440), E422 (= E478)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39

6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
25% identity, 50% coverage: 46:516/936 of query aligns to 48:460/466 of 6lppA

query
sites
6lppA

Q

K

V

V

V

L

A

L

E

R

V

P

R

R

D

T

E

S

A

E

E

E

V

V

K

S

G

H

V

I

L

L

A

R

A

H

C

C

R

H

R

E

H

R

G

N

A

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

D

V

S

F

V

D

L

E

M

I

I

I

L

L

G

S

T

T

G

A

W

R

T

M

Q

N

A

R

V

V

V

L

E

S

D

F

E

H

G

S

K

V

R

S

-

G

-

I

I

L

R

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

R

Y

L

V

A

E

F

R

A

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

D

G

S

S

C
|
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
|
A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

S

Y

D

G

N

S

S

L

Y

H

Q

G

T

T

V

V

M

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

V

L

D

D

T

C

G

-

N

-

P

-

E

-

S

-

V

L

A

T

A

S

F

L

R

R

A

-

S

-

H

-

A

-

E

-

L

-

S

-

R

-

L

-

D

-

M

-

G

-

R

-

V

-

R

-

D

-

E

-

T

-

L

-

A

-

G

-

R

-

I

-

R

-

H

-

K

-

F

-

A

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

V

-

D

-

F

-

T

-

D

-

P

-

L

-

D

-

I

-

L

-

T

-

H

Q

L

L

M

F

I

I

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F
x
I

I
|
I

A

T

E

T

I

V

T

S

Y

I

R

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

S

V

A

A

L

F

L

L

L

G

F

C

P

P

D

G

I

F

A

A

E

E

A

V

C

L

R

Q

A

T

V

F

V

S

A

T

L

C

K

K

Q

G

A

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

V

S

C

L

M

R

Q

C

L

V

V

E

G

D

R

K

H

P

L

G

H

M

L

P

A

A

S

Q

P

I

V

R

Q

G

-

L

-

A

-

D

E

G

S

V

P

T

F

S

Y

L

V

L

L

V

I

E

E

A

T

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G

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N

A

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P

binding (2R)-2-hydroxypentanedioic acid: M82 (≠ L80), R333 (= R380), T337 (≠ F384), K348 (= K392), Y379 (≠ F431), H381 (= H433), H388 (= H440), N390 (≠ T442), E422 (= E478), H423 (= H479)
binding flavin-adenine dinucleotide: P75 (≠ F73), Q76 (≠ R74), G77 (≠ A75), G78 (≠ A76), N79 (≠ G77), T80 (= T78), G81 (≠ S79), M82 (≠ L80), G85 (≠ Q83), S86 (≠ A84), L139 (≠ P135), G140 (≠ A136), A141 (≠ S137), C145 (= C141), H146 (≠ K142), G148 (= G144), G149 (= G145), N150 (≠ I146), A152 (= A148), T153 (≠ N149), A155 (= A151), G156 (≠ S152), G157 (= G153), E206 (= E253), G207 (= G254), I211 (≠ F258), I212 (= I259), H381 (= H433), E422 (= E478), H423 (= H479), N459 (= N515)
binding zinc ion: H381 (= H433), H388 (= H440), E422 (= E478)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 39

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
26% identity, 50% coverage: 56:520/936 of query aligns to 51:456/459 of P9WIT1

query
sites
P9WIT1

E

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A