SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011386526.1 AMB_RS21140 acetate--CoA ligase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
76% identity, 100% coverage: 1:644/645 of query aligns to 1:645/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G261) mutation to I: Loss of activity.
G266 (= G264) mutation to I: Great decrease in activity.
K269 (= K267) mutation to G: Great decrease in activity.
E414 (= E412) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
68% identity, 100% coverage: 1:642/645 of query aligns to 1:646/652 of P27550

query
sites
P27550

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K609 (= K605) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
68% identity, 99% coverage: 5:642/645 of query aligns to 7:646/652 of Q8ZKF6

query
sites
Q8ZKF6

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Y

Y

N

E

E

T

W

K

Y

Y

E

K

R

Q

S

S

V

I

K

N

D

D

P

P

D

D

G

T

F

F

W

W

G

G

E

E

H

Q

G

G

K

K

R

I

I

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

V

T

S

S

Y

F

S

A

-

P

G

G

D

N

V

V

S

S

I

I

K

K

W

W

F

Y

E

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

R

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

E

Q

S

S

A

K

H

H

I

I

T

S

Y

Y

G

R

D

E

L

L

H

H

E

R

R

D

V

V

C

C

R

R

L

F

A

A

N

N

A

T

M

L

T

L

D

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

V

V

H

H

S

S

I

V

V

I

F

F

G

G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

G

G

R

R

I

I

Q

I

D

D

C

S

D

S

S

S

S

R

L

L

L

V

I

I

T

T

A

A

D

D

E

E

G

G

L

V

R

R

G

A

G

G

R
|
R

K

S

V

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

K

D

A

A

L

L

E

K

T

N

-

P

-

N

C

V

W

T

S

S

C

V

K

E

S

H

V

V

I

I

V

V

V

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

H

D

M

W

V

Q

T

E

G

G

R

R

D

D

H

L

W

W

Y

W

H

R

D

D

L

L

V

I

A

E

K

K

A

A

A

S

P

P

T

E

H

H

T

Q

P

P

V

E

E

A

M

M

S

N

A

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

E

P

G

G

D

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

I

T

T

T

L

L

M

M

F

F

E

E

G

G

I

V

P

P

N
|
N

Y

W

P

P

T

T

V

P

S

A

R

R

F

M

W

C

D

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

R

A

E

E

G

G

E

D

E

K

P

A

V

I

K

E

K

G

T

T

S

D

R

R

A

S

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

H

W

R

K

V

K

V

I

G

G

D

K

G

E

R

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

M

L

V

V

D

D

A

N

E

E

G

G

K

H

T

P

L

Q

E

E

G

G

A

A

V

T

E

E

G

G

N

N

L

L

C

V

L

I

A

T

E

D

P

-

G

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

L

L

W

F

G

G

D

D

H

H

E

E

R

R

F

F

K

E

Q

Q

S

T

Y

Y

F

F

A

S

T

T

Y

F

Q

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D

D

G

G

A

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I

I

T

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

A

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

V

N

A

H

G

G

E

E

P

P

S

S

E

P

D

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

L

V

I

L

Q

H

W

W

S

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

F

T

G

S

S

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

D

E

S

E

R

K

M

Q

R194 (= R191) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T306) binding
N335 (= N330) binding
A357 (= A352) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D513) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S519) binding
G524 (= G520) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R522) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R580) binding ; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K605) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
68% identity, 99% coverage: 5:642/645 of query aligns to 3:639/640 of 5jrhA

query
sites
5jrhA

Y

H

P

A

V

I

P

P

A

A

E

N

T

I

A

A

K

D

N

R

A

C

L

L

I

I

D

N

N

P

D

E

K

Q

Y

Y

N

E

E

T

W

K

Y

Y

E

K

R

Q

S

S

V

I

K

N

D

D

P

P

D

D

G

T

F

F

W

W

G

G

E

E

H

Q

G

G

K

K

R

I

I

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

V

T

S

S

Y

F

S

A

-

P

G

G

D

N

V

V

S

S

I

I

K

K

W

W

F

Y

E

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

R

G

D

D

Q

R

T

T

A

A

I

I

W
|
W

E

E

G

G

D

D

P

T

N

S

E

Q

S

S

A
x
K

H

H

I

I

T

S

Y

Y

G

R

D

E

L

L

H

H

E

R

R

D

V

V

C

C

R

R

L

F

A

A

N

N

A

T

M

L

T

L

D

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I
x
V

P

P

E
|
E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

V

V

H

H

S

S

I

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

G
|
G

R
|
R

I

I

Q

I

D

D

C

S

D

S

S

S

S

R

L

L

L

V

I

I

T

T

A

A

D

D

E

E

G

G

L

V

R
|
R

G

A

G

G

R

R

K

S

V
x
I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

K

D

A

A

L

L

E

K

T

N

-

P

-

N

C

V

W

T

S

S

C

V

K

E

S

H

V

V

I

I

V

V

V
x
L

K

K

R
|
R

T

T

G

G

G

S

D

D

I

I

H

D

M

W

V

Q

T

E

G

G

R

R

D

D

H

L

W

W

Y

W

H

R

D

D

L

L

V

I

A

E

K

K

A

A

A

S

P

P

T

E

H

H

T

Q

P

P

V

E

E

A

M

M

S

N

A

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y
|
Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

E

P

G

G

D

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D
|
D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

I

T

T

T

L

L

M

M

F

F

E

E

G

G

I

V

P

P

N

N

Y

W

P

P

T

T

V

P

S

A

R

R

F

M

W

C

D

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

R

A

E

E

G

G

E

D

E

K

P

A

V

I

K

E

K

G

T

T

S

D

R

R

A

S

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

H

W

R

K

V

K

V

I

G

G

D

K

G

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

M

L

V

V

D

D

A

N

E

E

G

G

K

H

T

P

L

Q

E

E

G

G

A

A

V

T

E

E

G

G

N

N

L

L

C

V

L

I

A

T

E

D

P

-

G

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

L

L

W

F

G

G

D

D

H

H

E

E

R

R

F

F

K

E

Q

Q

S

T

Y

Y

F

F

A

S

T

T

Y

F

Q

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H
|
H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

A

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

V

N

A

H

G

G

E

E

P

P

S

S

E

P

D

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

L

V

I

L

Q

H

W

W

S

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

F

-

G

-

S

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

D

E

S

E

R

K

M

Q

active site: T260 (= T259), T412 (= T411), E413 (= E412), N517 (= N517), R522 (= R522), K605 (= K605)
binding (r,r)-2,3-butanediol: W93 (= W94), K102 (≠ A103), V138 (≠ I139), E140 (= E141), G169 (= G170), R170 (= R171), L216 (≠ V215), R218 (= R217), Y259 (= Y258), K266 (= K265), P267 (= P266)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G382), E384 (= E383), P385 (= P384), D407 (= D406), T408 (= T407), W409 (= W408), W410 (= W409), Q411 (= Q410), T412 (= T411), D496 (= D496), I508 (= I508), R511 (= R511), N517 (= N517), R522 (= R522)
binding coenzyme a: F159 (= F160), G160 (= G161), G161 (= G162), R187 (= R188), I192 (≠ V193), D302 (= D301), S519 (= S519), H521 (= H521), R580 (= R580), P585 (= P585)
binding magnesium ion: V533 (= V533), H535 (= H535), I538 (≠ V538)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
68% identity, 99% coverage: 5:642/645 of query aligns to 3:640/641 of 2p20A

query
sites
2p20A

Y

H

P

A

V

I

P

P

A

A

E

N

T

I

A

A

K

D

N

R

A

C

L

L

I

I

D

N

N

P

D

E

K

Q

Y

Y

N

E

E

T

W

K

Y

Y

E

K

R

Q

S

S

V

I

K

N

D

D

P

P

D

D

G

T

F

F

W

W

G

G

E

E

H

Q

G

G

K

K

R

I

I

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

V

T

S

S

Y

F

S

A

-

P

G

G

D

N

V

V

S

S

I

I

K

K

W

W

F

Y

E

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

R

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

E

Q

S

S

A

K

H

H

I

I

T

S

Y

Y

G

R

D

E

L

L

H

H

E

R

R

D

V

V

C

C

R

R

L

F

A

A

N

N

A

T

M

L

T

L

D

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

V

V

H

H

S

S

I

V

V

I

F

F

G

G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

G

G

R

R

I

I

Q

I

D

D

C

S

D

S

S

S

S

R

L

L

L

V

I

I

T

T

A

A

D

D

E

E

G

G

L

V

R

R

G

A

G

G

R

R

K

S

V

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

K

D

A

A

L

L

E

K

T

N

-

P

-

N

C

V

W

T

S

S

C

V

K

E

S

H

V

V

I

I

V

V

V

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

H

D

M

W

V

Q

T

E

G

G

R

R

D

D

H

L

W

W

Y

W

H

R

D

D

L

L

V

I

A

E

K

K

A

A

A

S

P

P

T

E

H

H

T

Q

P

P

V

E

E

A

M

M

S

N

A

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

E

P

G

G

D

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V
|
V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

I

T

T

T

L

L

M

M

F

F

E

E

G

G

I

V

P

P

N

N

Y

W

P

P

T

T

V

P

S

A

R

R

F

M

W

C

D

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

R

A

E

E

G

G

E

D

E

K

P

A

V

I

K

E

K

G

T

T

S

D

R

R

A

S

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

H

W

R

K

V

K

V

I

G

G

D

K

G

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

M

L

V

V

D

D

A

N

E

E

G

G

K

H

T

P

L

Q

E

E

G

G

A

A

V

T

E

E

G

G

N

N

L

L

C

V

L

I

A

T

E

D

P

-

G

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

L

L

W

F

G

G

D

D

H

H

E

E

R

R

F

F

K

E

Q

Q

S

T

Y

Y

F

F

A

S

T

T

Y

F

Q

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

A

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

V

N

A

H

G

G

E

E

P

P

S

S

E

P

D

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

W

W

V

V

R

A

K

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

L

V

I

L

Q

H

W

W

S

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

F

-

G

-

S

T

L

S

G

N

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

D

E

S

E

R

K

M

Q

active site: T260 (= T259), T412 (= T411), E413 (= E412), N517 (= N517), R522 (= R522), K605 (= K605)
binding adenosine-5'-monophosphate-propyl ester: V306 (= V305), T307 (= T306), G383 (= G382), E384 (= E383), P385 (= P384), D407 (= D406), T408 (= T407), W409 (= W408), W410 (= W409), Q411 (= Q410), T412 (= T411), D496 (= D496), I508 (= I508), R511 (= R511), N517 (= N517), R522 (= R522)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
68% identity, 99% coverage: 5:642/645 of query aligns to 2:636/637 of 2p2fA

query
sites
2p2fA

Y

H

P

A

V

I

P

P

A

A

E

N

T

I

A

A

K

D

N

R

A

C

L

L

I

I

D

N

N

P

D

E

K

Q

Y

Y

N

E

E

T

W

K

Y

Y

E

K

R

Q

S

S

V

I

K

N

D

D

P

P

D

D

G

T

F

F

W

W

G

G

E

E

H

Q

G

G

K

K

R

I

I

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

V

T

S

S

Y

F

S

A

-

P

G

G

D

N

V

V

S

S

I

I

K

K

W

W

F

Y

E

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

R

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

E

Q

S

S

A

K

H

H

I

I

T

S

Y

Y

G

R

D

E

L

L

H

H

E

R

R

D

V

V

C

C

R

R

L

F

A

A

N

N

A

T

M

L

T

L

D

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

V

V

H

H

S

S

I

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

G

G

R

R

I

I

Q

I

D

D

C

S

D

S

S

S

S

R

L

L

L

V

I

I

T

T

A

A

D

D

E

E

G

G

L

V

R
|
R

G

A

G

G

R

R

K

S

V
x
I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

K

D

A

A

L

L

E

K

T

N

-

P

-

N

C

V

W

T

S

S

C

V

K

E

S

H

V

V

I

I

V

V

V

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

H

D

M

W

V

Q

T

E

G

G

R

R

D

D

H

L

W

W

Y

W

H

R

D

D

L

L

V

I

A

E

K

K

A

A

A

S

P

P

T

E

H

H

T

Q

P

P

V

E

E

A

M

M

S

N

A

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

E

P

G

G

D

D

I

I

Y

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

I

T

T

T

L

L

M

M

F

F

E

E

G

G

I
x
V

P
|
P

N

N

Y

W

P

P

T

T

V

P

S

A

R

R

F

M

W

C

D

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T
|
T

A
|
A

I

I

R

R

S

A

L

L

M

M

R

A

E

E

G

G

E

D

E

K

P

A

V

I

K

E

K

G

T

T

S

D

R

R

A

S

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

H

W

R

K

V

K

V

I

G

G

D

K

G

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

M

L

V

V

D

D

A

N

E

E

G

G

K

H

T

P

L

Q

E

E

G

G

A

A

V

T

E

E

G

G

N

N

L

L

C

V

L

I

A

T

E

D

P

-

G

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

L

L

W

F

G

G

D

D

H

H

E

E

R

R

F

F

K

E

Q

Q

S

T

Y

Y

F

F

A

S

T

T

Y

F

Q

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

A

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

V

N

A

H

G

G

E

E

P

P

S

S

E

P

D

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

L

V

I

L

Q

H

W

W

S

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

F

-

G

-

S

-

L

-

G

-

D

T

T

S

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

D

E

S

E

R

K

M

Q

active site: T259 (= T259), T411 (= T411), E412 (= E412), N516 (= N517), R521 (= R522), K604 (= K605)
binding adenosine monophosphate: G382 (= G382), E383 (= E383), P384 (= P384), D406 (= D406), T407 (= T407), W408 (= W408), W409 (= W409), Q410 (= Q410), T411 (= T411), D495 (= D496), I507 (= I508), R510 (= R511), N516 (= N517), R521 (= R522)
binding coenzyme a: F158 (= F160), G159 (= G161), G160 (= G162), R186 (= R188), I191 (≠ V193), A300 (= A300), W304 (= W304), T306 (= T306), V328 (≠ I328), P329 (= P329), A352 (= A352), T354 (= T354), A355 (= A355), S518 (= S519), G519 (= G520), R579 (= R580), P584 (= P585)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
67% identity, 99% coverage: 5:642/645 of query aligns to 3:633/634 of 1pg3A

query
sites
1pg3A

Y

H

P

A

V

I

P

P

A

A

E

N

T

I

A

A

K

D

N

R

A

C

L

L

I

I

D

N

N

P

D

E

K

Q

Y

Y

N

E

E

T

W

K

Y

Y

E

K

R

Q

S

S

V

I

K

N

D

D

P

P

D

D

G

T

F

F

W

W

G

G

E

E

H

Q

G

G

K

K

R

I

I

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

V

T

S

S

Y

F

S

A

-

P

G

G

D

N

V

V

S

S

I

I

K

K

W

W

F

Y

E

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

A

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

K

E

R

N

R

G

D

D

Q

R

T

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

N

S

E

Q

S

S

A

K

H

H

I

I

T

S

Y

Y

G

R

D

E

L

L

H

H

E

R

R

D

V

V

C

C

R

R

L

F

A

A

N

N

A

T

M

L

T

L

D

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

V

I

G

G

A

A

V

V

H

H

S

S

I

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

D

E

A

A

L

V

A

A

G

G

R

C

I

I

Q

I

D

D

C

S

D

S

S

S

S

R

L

L

L

V

I

I

T

T

A

A

D

D

E

E

G

G

L

V

R
|
R

G

A

G

G

R
|
R

K

S

V
x
I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

K

D

A

A

L

L

E

K

T

N

-

P

-

N

C

V

W

T

S

S

C

V

K

E

S

H

V

V

I

I

V

V

V

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

H

D

M

W

V

Q

T

E

G

G

R

R

D

D

H

L

W

W

Y

W

H

R

D

D

L

L

V

I

A

E

K

K

A

A

A

S

P

P

T

E

H

H

T

Q

P

P

V

E

E

A

M

M

S

N

A

A

E

E

D

D

P

P

L

L

F

F

L

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

E

P

G

G

D

D

I

I

Y

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W

W

V
|
V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

I

T

T

T

L

L

M

M

F

F

E

E

G

G

I
x
V

P
|
P

N
|
N

Y

W

P

P

T

T

V

P

S

A

R

R

F

M

W

C

D

Q

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T
|
T

A
|
A

I

I

R

R

S

A

L

L

M

M

R

A

E

E

G

G

E

D

E

K

P

A

V

I

K

E

K

G

T

T

S

D

R

R

A

S

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V
|
V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

T

E

W

W

Y

Y

H

W

R

K

V

K

V

I

G

G

D

K

G

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

M

L

V

V

D

D

A

N

E

E

G

G

K

H

T

P

L

Q

E

E

G

G

A

A

V

T

E

E

G

G

N

N

L

L

C

V

L

I

A

T

E

D

P

-

G

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

L

L

W

F

G

G

D

D

H

H

E

E

R

R

F

F

K

E

Q

Q

S

T

Y

Y

F

F

A

S

T

T

Y

F

Q

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G
|
G

H
|
H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

A

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

T

T

L

L

V

N

A

H

G

G

E

E

P

P

S

S

E

P

D

E

L

L

R

Y

K

A

E

E

L

V

V

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

L

V

I

L

Q

H

W

W

S

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

N

G

D

D

F

-

G

-

S

-

L

-

G

-

D

-

T

-

S

-

T

-

L

L