SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011386821.1 NCBI__GCF_000009985.1:WP_011386821.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
39% identity, 85% coverage: 30:413/453 of query aligns to 51:413/444 of Q8NLB7

query
sites
Q8NLB7

I

V

I

I

F

F

D
|
D

G

G

Y

Y

D
|
D

L

L

V

I

I

V

Y

Y

G

G

V

T

V

V

L

Q

P

S

V

A

L

L

M

A

K

K

E

E

W

W

H

N

L

L

D

S

P

S

I

A

Q

T

A

L

G

G

T

T

L

I

G

G

S

S

Y

T

A

A

L

F

F

F

G

G

M

M

M

A

F

I

G

G

A

A

L

V

T

F

F

I

G

G

S

R

L

L

S

S

D

D

R
|
R

F

V

G

G

R

R

K

K

P

A

V

A

I

V

A

I

V

G

C

S

V

V

V

L

L

I

F

L

S

S

L

V

F

F

T

T

V

M

L

L

N

C

G

A

F

F

V

A

R

P

D

N

T

P

T

W

E

V

F

F

A

G

I

A

C

F

R

R

F

F

V

I

A

A

G

G

L

L

G

G

I

L

G

G

V

L

M

V

P

P

N

S

A

V

V

N

A

A

L

M

M

T

T

S

E

D

Y

L

A

V

P

P

K

R

R

K

L

T

R

M

S

S

T

A

L

W

V

A

A

T

V

V

M

M

F

M

S

S

G

G

Y

V

S

P

V

I

G

G

M

S

L

I

S

A

A

A

G

V

L

L

G

A

M

L

T

V

L

V

I

V

P

P

V

S

-

S

-

E

Y

W

G

G

W

W

S

R

A

F

V

M

F

F

F

L

V

I

A

A

A

L

V

I

P

P

L

L

L

V

-

G

L

L

P

P

V

I

I

A

L

M

F

K

L

V

L

I

P

P

E

-

S

-

I

-

G

-

F

-

L

-

I

-

K

-

A

-

G

-

R

-

T

-

A

-

E

-

A

-

A

S

D

D

K

K

L

A

A

I

R

K

L

A

E

D

P

H

S

D

Y

I

K

R

P

-

Q

E

P

G

G

H

D

D

E

E

I

-

S

-

F

-

P

-

T

-

G

-

A

-

T

-

G

-

Q

P

A

A

S

G

L

F

P

K

A

D

L

L

F

L

A

V

G

D

G

R

R

Y

G

R

V

W

S

I

T

S

V

I

M

W

F

F

W

A

I

L

A

A

F

T

F

F

M

V

C

T

L

L

M

A

V

W

Y

Y

A

G

L

L

G

G

S

T

W

W

L

L

P

P

K

R

L

L

M

M

N

E

N

T

A

A

G

G

Y

Y

G

E

L

F

G

G

S

H

S

A

L

L

S

M

F

F

L

T

L

L

V

A

L

L

N

N

F

L

G

G

A

A

I

V

F

I

G

G

A

S

V

V

G

V

G

T

G

A

H
x
W

I

A

A

G

D
|
D

R
|
R

M

F

N

G

L

P

K
x
I

R

R

V

S

L

G

V

V

A

I

F

A

V

G

I

I

A

A

A

G

I

I

S

A

I

L

S

L

L

L

G

L

V

T

K

Y

S

P

P

P

T

V

L

T

V

A

L

V

Y

Y

V

V

L

I

V

L

A

I

V

L

A

A

G

G

A

V

T

G

T

T

I

H

G

G

T

T

Q

Q

I

I

L

L

L

I

Y

I

A

A

Y

A

V

V

A

A

Q

N

F

F

Y

Y

P

P

M

S

A

N

I

L

R

R

S

G

T

T

G

A

I

L

G

G

W

W

A

A

S

L

G

G

V

V

G

G

R
|
R

S

I

G

G

A

A

I

V

L

V

G

A

P

P

V

Q

L

L

G

A

G

G

T

L

L

L

A

A

W

W

K

N

L

L

P

G

L

V

M

N

Q

S

N

N

F

F

M

I

A

M

F

F

D54 (= D33) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D36) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R82) mutation to A: Loss of transport activity.
W309 (≠ H309) mutation to V: Loss of transport activity.
D312 (= D312) mutation to A: Loss of transport activity.
R313 (= R313) mutation to A: Loss of transport activity.
I317 (≠ K317) mutation I->H,Y: Loss of transport activity.
R386 (= R386) mutation to A: Loss of transport activity.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
35% identity, 95% coverage: 7:435/453 of query aligns to 8:441/452 of Q5EXK5

query
sites
Q5EXK5

H

Q

K

K

L

L

I

L

D

N

E

A

A

A

K

P

F

V

G

G

K

A

F

R

H

Q

W

W

N

R

V

V

L

I

F

I

W

C

T

C

A

F

L

L

I

V

I

M

F

L

D

D

G

G

Y

F

D

D

L

T

V

A

I

A

Y

I

G

G

V

F

V

I

L

A

P

P

V

D

L

I

M

R

K

S

E

H

W

W

H

Q

L

L

D

T

P

A

I

G

Q

D

A

L

G

A

T

P

L

L

G

F

S

G

Y

A

A

G

L

L

F

L

G

G

M

L

M

T

F

A

G

G

A

A

L

L

T

L

F

C

G

G

S

P

L

L

S

S

D
|
D

R

R

F

F

G

G

R

R

K

K

P

R

V

V

I

I

A

E

V

F

C

C

V

V

V

A

L

L

F

F

S

G

L

L

F

F

T

S

V

L

L

L

N

S

G

A

F

F

V

A

R

P

D

D

T

L

T

Q

E

M

F

L

A

V

I

F

C

L

R

R

F

F

V

L

A

T

G

G

L

L

G

G

I

L

G

G

V

A

M

M

P

P

N

N

A

T

V

I

A

T

L

M

M

T

T

S

E

E

Y

Y

A

L

P

P

K

A

R

R

L

R

R

R

S

G

T

A

L

L

V

V

A

T

V

L

M

M

F

F

S

C

G

G

Y

F

S

T

V

L

G

G

G

S

M

A

L

F

S

G

A

G

G

V

L

V

G

S

M

A

T

Q

L

L

I

V

P

P

V

L

Y

I

G

G

W

W

S

H

A

G

V

I

F

L

F

V

V

L

A

G

V

V

-

L

P

P

L

L

L

I

L

L

L

A

P

V

V

A

I

L

L

V

F

P

L

L

P

P

E

E

S

S

I

P

G

R

F

W

L

Q

I

I

K

-

A

-

G

-

R

R

T

R

A

Q

E

L

A

P

A

Q

D

A

K

T

L

I

A

A

R

R

L

T

E

V

P

G

S

A

Y

I

K

T

P

G

Q

E

P

R

G

Y

D

N

E

D

I

T

S

H

F

F

-

W

-

L

-

D

P

E

T

P

G

A

A

A

T

G

G

A

Q

K

A

G

S

S

L

I

P

S

A

Q

L

L

F

F

A

A

G

G

G

R

R

Q

G

L

V

A

S

I

T

T

V

L

M

M

F

L

W

W

I

V

A

V

F

F

M

M

C

S

L

L

M

I

V

I

Y

Y

A

L

L

L

G

S

S

S

W

W

L

M

P

P

K

T

L

L

M

L

N

N

N

H

A

R

G

G

Y

I

G

D

L

L

G

Q

S

H

S

A

L

S

S

W

F

V

L

T

L

A

V

A

L

F

N

Q

F

I

G

G

A

G

I

T

F

F

G

G

A

A

V

L

G

L

G

G

H
x
V

I

L

A

M

D
|
D

R

R

M

F

N

N

L

P

K

Y

R

R

V

V

L

L

V

A

A

L

F

S

F

Y

V

G

I

L

A

G

A

A

I

V

S

C

I

I

S

V

L

M

L

I

G

G

V

L

K

S

S

E

P

N

T

G

L

L

V

W

L

L

Y

M

V

A

L

L

V

A

A

I

V

F

A

G

G

T

A

G

T

I

T

G

I

I

-

S

G

G

T

S

Q

Q

I

V

L

G

L

L

Y

N

A

A

Y

L

V

T

A

A

Q

T

F

L

Y

Y

P

P

M

T

A

Q

I

S

R

R

S

A

T

T

G

G

I

V

G

S

W

W

A

S

S

N

G

A

V

V

G

G

R

R

S

C

G

G

A

A

I

I

L

V

G

G

P

S

V

L

L

S

G

G

T

V

L

M

A

A

W

M

K

N

L

F

P

S

L

F

M

D

Q

T

N

L

F

F

M

F

A

V

F

I

A

A

V

V

P

P

G

A

V

V

I

V

A

S

A

A

-

V

-

M

-

L

I

I

A

L

M

L

S

T

L

L

V

A

V

V

R

R

G

N

A

P

Q

T

H

S

A

V

P

P

D82 (= D81) mutation to A: Loss of activity.
V311 (≠ H309) mutation to W: Loss of activity.
D314 (= D312) mutation to A: Loss of activity.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
34% identity, 95% coverage: 4:434/453 of query aligns to 12:445/448 of Q51955

query
sites
Q51955

V

L

N

D

A

V

H

Q

K

S

L

F

I

I

D

N

E

Q

A

Q

K

P

F

L

G

S

K

R

F

Y

H

Q

W

W

N

R

V

V

L

V

F

L

W

L

T

C

A

F

L

L

I

I

I

V

I

F

F

L

D
|
D

G

G

Y

L

D
|
D

L

T

V

A

I

A

Y

M

G

G

V

F

V

I

L

A

P

P

V

A

L

L

M

S

K

Q

E

E

W

W

H

G

L

I

D

D

P

R

I

A

Q

S

A

L

G

G

T

P

L

V

G

M

S

S

Y

A

A

A

L

L

F

I

G

G

M

M

M

V

F

F

G

G

A

A

L

L

T

G

F

S

G
|
G

S

P

L

L

S

A

D
|
D

R

R

F

F

G
|
G

R

R

K

K

P

G

V

V

I

L

A

V

V

G

C

A

V

V

V

L

L

V

F

F

S

G

L

G

F

F

T

S

V

L

L

A

N

S

G

A

F

Y

V

A

R

T

D

N

T

V

T

D

E

Q

F

L

A

L

I

V

C

L

R
|
R

F

F

V

L

A

T

G

G

L

L

G

G

I

L

G

G

G

A

V

G

M

M

P

P

N

N

A

A

V

T

A

T

L

L

M

L

T

S

E
|
E

Y

Y

A

T

P

P

K

E

R

R

L

L

R

K

S

S

T

L

L

L

V

V

A

T

V

S

M

M

F

F

S

C

G

G

Y

F

S

N

V

L

G

G

G

M

M

A

L

G

S

G

A

G

G

F

L

I

G

S

M

A

T

K

L

M

I

I

P

P

V

A

Y

Y

G

G

W

W

S
x
H

A

S

V

L

F

L

F

V

V

I

A

G

-

V

V

L

P

P

L

L

L

A

P

L

V

V

I

L

L

M

F

V

L

W

L

L

P

P

E

E

S

S

I

A

G

R

F

F

L

L

I

V

K

V

A

R

G

N

R

R

T

G

A

T

E

D

A

K

A

I

D

R

K

K

-

T

L

L

A

S

R

P

L

I

E

A

P

P

S

Q

Y

V

K

V

P

A

Q

E

P

A

G

G

D

S

-

F

E

S

I

V

S

P

F

E

P

Q

T

K

G

A

A

V

T

A

G

A

Q

R

A

S

S

V

L

F

P

A

A

V

L

I

F

F

A

S

G

G

G

T

R

Y

G

G

V

L

S

G

T

T

V

M

M

L

F

L

W

W

I

L

A

T

F

Y

F

F

M

M

C

G

L

L

L

V

M

I

V

V

Y

Y

A

L

L

L

G

T

S

S

W

W

L

L

P

P

K

T

L

L

M

M

N

R

N

D

A

S

G

G

Y

A

G

S

L

M

G

E

S

Q

S

A

L

A

S

F

F

I

L

G

L

A

V

L

L

F

N

Q

F

F

G

G

A

G

I

V

F

L

G

S

A

A

V

V

G

G

G

V

G

G

H

W

I

A

A

M

D
|
D

R

R

M

Y

N

N

L

P

K
x
H

R

K

V

V

L

I

V

G

A

I

F

F

F

Y

V

L

I

L

A

A

A

G

I

V

S

F

I

A

S

Y

L

A

L

V

G

G

V

Q

K

S

S

L

P

G

T

N

L

I

-

T

V

V

L

L

Y

A

V

T

L

L

V

V

A

L

V

I

A

A

G

G

A

M

T

C

T

V

I

N

G

G

T

A

Q

Q

I

S

L

A

L

M

Y

P

A

S

Y

L

V

A

A

A

Q

R

F

F

Y

Y

P

P

M

T

A

Q

I

G

R
|
R

S

A

T

T

G

G

I

V

G

S

W

W

A

M

S

L

G

G

V

I

G

G

R
|
R

S

F

G

G

A

A

I

I

L

L

G

G

P

A

V

W

L

S

G

G

G

A

T

T

L

L

A

G

W

L

K

G

L

W

P

N

L

F

M

E

Q

Q

N

V

F

L

M

T

A

A

F

L

A

L

V

V

P

P

G

A

V

A

I

L

A

A

A

T

I

V

A

G

M

-

S

-

L

V

V

I

V

V

R

K

G

G

-

L

A

V

Q

S

H
|
H

A

A

D41 (= D33) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D36) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G77) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D81) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G84) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R116) mutation to A: Abolishes 4-HBA transport.
E144 (= E136) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ S175) mutation to A: Decrease in 4-HBA transport and chemotaxis.
D323 (= D312) mutation to N: Abolishes 4-HBA transport and chemotaxis.
H328 (≠ K317) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
R386 (= R374) mutation to A: Strong decrease in 4-HBA transport.
R398 (= R386) mutation to A: Abolishes 4-HBA transport.
H444 (= H433) mutation to A: No change in 4-HBA transport and chemotaxis.

P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
32% identity, 87% coverage: 28:421/453 of query aligns to 22:383/403 of P77589

query
sites
P77589

L

L

I

V

I

A

I

L

F

M

D
x
E

G

G

Y

L

D

D

L

L

V

Q

I

A

Y

A

G

G

V

I

V

A

L

A

P

G

V

G

L

I

M

A

K

Q

E

A

W

F

H

A

L

L

D

D

P

K

I

M

Q

Q

A

M

G

G

T

W

L

I

G

F

S

S

Y

A

A

G

L

I

F

L

G

G

M

L

F

P

G

G

A

A

L

L

T

V

F

G

G

G

S

M

L

L

S

A

D
|
D

R

R

F

Y

G

G

R

R

K

K

P

R

V

I

I

L

A

I

V

G

C

S

V

V

V

A

L

L

F

F

S

G

L

L

F

F

T

S

V

L

L

A

N

T

G

A

F

I

V

A

R

W

D

D

T

F

T

P

E

S

F

L

A

V

I

F

C

A

R

R

F

L

V

M

A

T

G

G

L

V

G

G

I

L

G

G

G

A

V

A

M

L

P

P

N

N

A

L

V

I

A

A

L

L

M

T

T

S

E

E

Y

A

A

A

P

G

K

P

R

R

L

F

R

R

S

G

T

T

L

A

V

V

A

S

V

L

M

M

F

Y

S

C

G

G

Y

V

S

P

V

I

G

G

G

A

M

A

L

L

S

A

A

A

G

T

L

L

G

G

M

F

T

A

L

G

I

A

P

N

V

L

Y

-

G

A

W

W

S

Q

A

T

V

V

F

F

F

W

V

V

A

G

-

V

V

V

P

P

L

L

L

I

L

L

L

V

P

P

V

L

I

L

L

M

F

R

L

W

L

L

P

P

E

E

S

S

I

A

G

V

F

F

L

-

I

-

K

-

A

-

G

-

R

-

T

-

A

-

E

-

A

-

D

-

K

-

L

-

A

-

R

-

L

-

E

-

P

-

S

-

Y

-

K

-

P

-

Q

-

P

A

G

G

D

E

E

K

I

Q

S

S

F

A

P

P

T

-

G

-

A

-

T

-

G

-

Q

-

A

-

S

P

L

L

P

R

A

A

L

L

F

F

A

A

G

P

G

E

R

T

G

A

V

T

S

A

T

T

V

L

M

L

F

L

W

W

I

L

A

C

F

Y

F

F

M

F

C

T

L

L

M

V

V

V

Y

Y

A

M

L

L

G

I

S

N

W

W

L

L

P

P

K

L

L

L

M

L

N

V

N

E

A

Q

G

G

Y

F

G

Q

L

P

G

S

S

Q

S

A

L

A

S

G

F

V

L

M

L

F

V

A

L

L

N

Q

F

M

G

G

A

A

I

A

F

S

G

G

A

T

V

L

G

M

G

L

G

G

H
x
A

I

L

A

M

D

D

R
x
K

M

-

N

-

L

L

K

R

R

P

V

V

L

T

V

M

A

S

F

L

V

I

I

Y

A

S

A

G

I

M

S

L

I

A

S

S

L

L

G

A

V

L

K

G

S

T

P

V

T

S

L

S

V

F

L

N

Y

G

V

M

L

L

V

L

A

A

-

G

-

F

V

V

A

A

G

G

A

L

T

F

T

A

I

T

G

G

T

G

Q

Q

I

S

L

V

L

L

Y

Y

A

A

Y

L

V

A

A

P

Q

L

F

F

Y

Y

P

S

M

S

A

Q

I

I

R

R

S

A

T

T

G

G

I

V

G

G

W

T

A

A

S

V

G

A

V

V

G

G

R

R

S

L

G

G

A

A

I

M

L

S

G

G

P

P

V

L

L

L

G

A

G

G

T

K

L

M

L

L

A

A

W

L

K

G

L

T

P

G

L

T

M

V

Q

-

N

G

F

V

M

M

A

A

F

A

A

S

V

A

P

P

G

G

V

I

I

L

A

V

A

A

E27 (≠ D33) mutation to A: Lack of 3HPP transport activity.; mutation to D: Slight decrease in 3HPP transport activity.
D75 (= D81) mutation D->A,E: Lack of 3HPP transport activity.
A272 (≠ H309) mutation to H: 30% increase in 3HPP transport activity.
K276 (≠ R313) mutation to D: Lack of 3HPP transport activity.

8uo8A Synaptic vesicle glycoprotein 2B (see paper)
26% identity, 61% coverage: 6:280/453 of query aligns to 6:307/574 of 8uo8A

query
sites
8uo8A

A

A

H

H

K

Q

-

Y

-

E

-

T

L

I

I

M

D

D

E

E

A

C

K

G

F
x
H

G
|
G

K

R

F

F

H

Q

W
|
W

N

I

V

L

L

F

F
|
F

W

V

T

L

A

G

L

L

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

S

V

F

V

A

L

L

P

P

V

S

L

A

M

E

K

K

E

D

W

M

H

C

L

L

D

S

P

S

I

S

Q

K

A

K

G

G

T

M

L

L

G

G

S

M

Y

I

A

V

L

Y

F

L

G

G

M

M

F

A

G

G

A

A

L

F

T

I

F

L

G

G

S

G

L

L

S

A

D

D

R

K

F

L

G

G

R

R

K

K

P

R

V

V

I

L

A

S

V

M

C

S

V

L

V

A

L

V

F

N

S

A

L

S

F

F

T

A

V

S

L

L

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

A

F

F

A

L

I

F

C

C

R

R

F

L

V

I

A

S

G

G

L

I

G

G

I

I

G

G

V

A

M

L

P

P

N

I

A

V

V
x
F

A

A

L

Y

M

F

T

S

E

E

Y

F

A

L

P

S

K

R

R

E

L

K

R

R

S

G

T
x
E

L

H

V

L

A

S

V

W

M

L

F

G

S

I

G

F

Y
x
W

S

M

V
x
T

G

G

M

L

L

Y

S

A

A

S

G

A

L
x
M

G

A

M

W

T
x
S

L
x
I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

G

-

F

-

S

-

M

-

G

-

T

-

N

-
x
Y

-

H

-

F

-

H

-
x
S

-

W

A

R

V
|
V

F

F

-

V

F

I

V

V

A

C

A

A

V

L

P

P

L

C

L

T

L

V

L

S

P

M

V

V

I

A

L

L

F

K

L

F

L

M

P

P

E

E

S

S

I

P

G

R

F

F

L

L

I

L

K

E

A

M

G

G

R

K

T

H

A

D

E

E

A

A

A

W

D

M

K

I

L

L

A

K

R

Q

L

V

E

H

P

D

S

T

Y

N

K

M

P

R

Q

A

P

K

G

G

D

T

E

P

I

E

S

K

F

V

P

F

T

T

G

V

A

S

T

N

G

I

Q

K

A

T

S

P

L

F

P

K

A

T

L

I

F

F

A

K

-

Q

-

V

-

W

-

D

-

N

-

A

-

L

-

Y

-

C

-

V

-

M

G

G

G

P

R

Y

G

R

V

M

S

N

T

T

V

L

M

I

F

L

W

A

I

V

A

V

F
x
W

F

F

M

A

C

M

L

A

L

F

M

S

V
x
Y

Y
|
Y

A

G

L

L

G

T

S

V

W

W

L

F

P

P

K

D

L

M

M

I

N

R

binding [(2R)-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-2-propanoyloxy-propyl] propanoate: M167 (≠ L164), I171 (≠ L168), Y185 (vs. gap), S189 (vs. gap), V192 (= V177)
binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: E148 (≠ T145), T159 (≠ V156), S170 (≠ T167)
binding 1,2-didecanoyl-sn-glycero-3-[phospho-l-serine]: H18 (≠ F15), G19 (= G16), W23 (= W20), F27 (= F24)
binding (4R)-4-(2-chloro-2,2-difluoroethyl)-1-{[(4R)-2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl}pyrrolidin-2-one: F134 (≠ V131), W157 (≠ Y154), W288 (≠ F261), Y295 (≠ V268), Y296 (= Y269)

Sites not aligning to the query:

Q9Z2I6 Synaptic vesicle glycoprotein 2C; Synaptic vesicle protein 2C from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 45% coverage: 9:211/453 of query aligns to 141:356/727 of Q9Z2I6

query
sites
Q9Z2I6

L

I

I

I

D

Q

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

A

V

L

L

F

F

F

W

V

T

L

A

G

L

M

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

T

E

D

W

L

H

C

L

I

D

P

P

N

I

S

Q

G

A

S

G

G

T

W

L

L

G

G

S

S

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

F

F

W

G

G

S

G

L

L

S

A

D

D

R

K

F

V

G

G

R

R

K

K

P

Q

V

S

I

L

A

L

V

I

C

C

V

M

V

S

L

V

F

N

S

G

L

F

F

F

T

A

V

F

L

L

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

F

F

F

A

L

I

L

C

C

R

R

F

L

V

L

A

S

G

G

L

F

G

G

I

I

G

G

V

A

M

I

P

P

N

T

A

V

V

F

A

S

L

Y

M

F

T

A

E

E

Y

V

A

L

P

A

K

R

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F

C

S

M

G

F

Y

W

S

M

V

I

G

G

M

I

L

Y

S

A

A

S

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

S

-

F

-

S

-

M

-

G

-

S

-

A

-

Y

-

Q

-

F

-

H

-

S

-

W

A

R

V

V

F

F

-

V

F

I

V

V

A

C

A

A

V

L

P

P

L

C

L

V

L

S

P

V

V

V

I

A

L

L

F

T

L

F

L

M

P

P

E

E

S

S

I

P

G

R

F

F

L

L

I

L

K

E

A

V

G

G

R

K

T

H

A

D

E

E

A

A

Sites not aligning to the query:

1:57 Interaction with SYT1
529:566 (Microbial infection) C.botulinum neurotoxin type A-binding
559 N→A: Loss of one glycosylation site. No effect on C.botulinum neurotoxin type A (BoNT/A, botA) binding, but reduces the uptake of BoNT/A.

Q496J9 Synaptic vesicle glycoprotein 2C from Homo sapiens (Human) (see 4 papers)
31% identity, 45% coverage: 9:211/453 of query aligns to 141:356/727 of Q496J9

query
sites
Q496J9

L

I

I

I

D

Q

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

A

V

L

L

F

F

F

W

V

T

L

A

G

L

M

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

T

E

D

W

L

H

C

L

I

D

P

P

N

I

S

Q

G

A

S

G

G

T

W

L

L

G

G

S

S

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

F

F

W

G

G

S

G

L

L

S

A

D

D

R

K

F

V

G

G

R

R

K

K

P

Q

V

S

I

L

A

L

V

I

C

C

V

M

V

S

L

V

F

N

S

G

L

F

F

F

T

A

V

F

L

L

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

F

F

F

A

L

I

F

C

C

R

R

F

L

V

L

A

S

G

G

L

F

G

G

I

I

G

G

V

A

M

I

P

P

N

T

A

V

V

F

A

S

L

Y

M

F

T

A

E

E

Y

V

A

L

P

A

K

R

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F

C

S

M

G

F

Y

W

S

M

V

I

G

G

M

I

L

Y

S

A

A

S

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

S

-

F

-

S

-

M

-

G

-

S

-

A

-

Y

-

Q

-

F

-

H

-

S

-

W

A

R

V

V

F

F

-

V

F

I

V

V

A

C

A

A

V

L

P

P

L

C

L

V

L

S

P

V

V

V

I

A

L

L

F

T

L

F

L

M

P

P

E

E

S

S

I

P

G

R

F

F

L

L

I

L

K

E

A

V

G

G

R

K

T

H

A

D

E

E

A

A

Sites not aligning to the query:

519:563 (Microbial infection) C.botulinum neurotoxin type A-binding
534 modified: carbohydrate, N-linked (GlcNAc...) asparagine
559 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: No change in interaction with C.botulinum neurotoxin type A heavy chain (botA, BoNT/A HC). Decreased molecular weight probably due to glycosylation loss, decreased interaction with BoNT/A HC.; N→Q: Decreased molecular weight probably due to glycosylation loss, decreased binding to BoNT/A HC. Greater reduction in weight; when associated with Q-565.
561 S→A: Decreased molecular weight probably due to glycosylation loss, decreased binding to BoNT/A HC.
563 F→A: No longer interacts with BoNT/A HC.
565 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Decreased molecular weight probably due to glycosylation loss, no change in binding to BoNT/A heavy chain. Greater reduction in weight; when associated with Q-559.

Q02563 Synaptic vesicle glycoprotein 2A; Synaptic vesicle protein 2; Synaptic vesicle protein 2A from Rattus norvegicus (Rat) (see 2 papers)
30% identity, 45% coverage: 9:211/453 of query aligns to 155:370/742 of Q02563

query
sites
Q02563

L

I

I

L

D

R

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

T

V

L

L

Y

F

F

W

V

T

L

A

G

L

L

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

K

E
x
D

W
x
M

H
x
C

L
|
L

D
x
S

P

D

I

S

Q

N

A

K

G

G

T

M

L

L

G

G

S

L

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

L

F

W

G

G

S

G

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

Q

V

C

I

L

A

L

V

I

C

S

V

L

V

S

L

V

F

N

S

S

L

V

F

F

T

A

V

F

L

F

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

T

F

F

A

L

I

F

C

C

R

R

F

L

V

L

A

S

G

G

L

V

G

G

I

I

G

G

V

S

M

I

P

P

N

I

A

V

V

F

A

S

L

Y

M

F

T

S

E

E

Y

F

A

L

P

A

K

Q

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F

C

S

M

G

F

Y

W

S

M

V

I

G

G

M

V

L

Y

S

A

A

A

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S
|
S

-
x
F

-
x
Q

-
x
M

-
x
G

-
x
S

-
x
A

-
x
Y

-
x
Q

-
x
F

-
x
H

-

S

-

W

A

R

V

V

F

F

-

V

F

L

V

V

A

C

A

A

V

F

P

P

L

S

L

V

L

F

L

A

P

I

V

G

I

A

L

L

F

T

L

T

L

Q

P

P

E

E

S

S

I

P

G

R

F

F

L

F

I

L

K

E

A

N

G

G

R

K

T

H

A

D

E

E

A

A

DMCLS 196:200 (≠ EWHLD 50:54) mutation Missing: No change in uptake of C.botulinum neurotoxin type D (BoNT/D, botD) or C.botulinum neurotoxin type E (BoNT/E).
321:331 (vs. 175:175, 9% identical) mutation Missing: No change in uptake of BoNT/D or BoNT/E.

Sites not aligning to the query:

498 N→Q: No change in uptake of BoNT/E or C.botulinum neurotoxin type A (BoNT/A, botA) by mouse SV2A/SV2B knockout neurons; SV2A apparent molecular weight decreases. No change in uptake of BoNT/E; when associated with Q-548. No change in uptake of BoNT/D.
548 N→Q: No change in uptake of BoNT/E or BoNT/A by mouse SV2A/SV2B knockout neurons; SV2A apparent molecular weight decreases. No change in uptake of BoNT/E; when associated with Q-498. No change in uptake of BoNT/D.
570:573 RLVN→TLVQ: Restores apparent molecular weight to wild-type, does not restore uptake of BoNT/E.
573 N→Q: BoNT/E not taken up by mouse SV2A/SV2B knockout neurons, decreased uptake of BoNT/A; SV2A apparent molecular weight decreases. No change in uptake of BoNT/D.

8k77A Synaptic vesicle glycoprotein 2A (see paper)
30% identity, 45% coverage: 9:211/453 of query aligns to 19:234/592 of 8k77A

query
sites
8k77A

L

I

I

L

D

R

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

T

V

L

L

Y

F

F

W

V

T

L

A

G

L

L

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

K

E

D

W

M

H

C

L

L

D

S

P

D

I

S

Q

N

A

K

G

G

T

M

L

L

G

G

S

L

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

L

F

W

G

G

S

G

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

Q

V

C

I

L

A

L

V

I

C

S

V

L

V

S

L

V

F

N

S

S

L

V

F

F

T

A

V

F

L

F

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

T

F

F

A

L

I

F

C

C

R

R

F

L

V

L

A

S

G

G

L

V

G

G

I

I

G

G

V

S

M

I

P

P

N

I

A

V

V

F

A

S

L

Y

M

F

T

S

E

E

Y

F

A

L

P

A

K

Q

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F

C

S

M

G

F

Y
x
W

S

M

V

I

G

G

M

V

L

Y

S

A

A

A

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

S

-

F

-

Q

-

M

-

G

-

S

-

A

-

Y

-

Q

-

F

-

H

-

S

-

W

A

R

V

V

F

F

-

V

F

L

V

V

A

C

A

A

V

F

P

P

L

S

L

V

L

F

L

A

P

I

V

G

I

A

L

L

F

T

L

T

L

Q

P

P

E

E

S

S

I

P

G

R

F

F

L

F

I

L

K

E

A

N

G

G

R

K

T

H

A

D

E

E

A

A

binding (2S)-2-[(4R)-2-oxidanylidene-4-propyl-pyrrolidin-1-yl]butanamide: W164 (≠ Y154)

Sites not aligning to the query:

binding (2S)-2-[(4R)-2-oxidanylidene-4-propyl-pyrrolidin-1-yl]butanamide: 311, 516, 520

8jlhA Cryo-em structure of sv2a dimer in complex with bont/a2 hc and levetiracetam (see paper)
30% identity, 45% coverage: 9:211/453 of query aligns to 19:234/592 of 8jlhA

query
sites
8jlhA

L

I

I

L

D

R

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

T

V

L

L

Y

F

F

W

V

T

L

A

G

L

L

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

K

E

D

W

M

H

C

L

L

D

S

P

D

I

S

Q

N

A

K

G

G

T

M

L

L

G

G

S

L

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

L

F

W

G

G

S

G

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

Q

V

C

I

L

A

L

V

I

C

S

V

L

V

S

L

V

F

N

S

S

L

V

F

F

T

A

V

F

L

F

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

T

F

F

A

L

I

F

C

C

R

R

F

L

V

L

A

S

G

G

L

V

G

G

I

I

G

G

V

S

M

I

P

P

N

I

A

V

V

F

A

S

L

Y

M

F

T

S

E

E

Y

F

A

L

P

A

K

Q

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F

C

S

M

G

F

Y
x
W

S

M

V

I

G

G

M

V

L

Y

S

A

A

A

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

S

-

F

-

Q

-

M

-

G

-

S

-

A

-

Y

-

Q

-

F

-

H

-

S

-

W

A

R

V

V

F

F

-

V

F

L

V

V

A

C

A

A

V

F

P

P

L

S

L

V

L

F

L

A

P

I

V

G

I

A

L

L

F

T

L

T

L

Q

P

P

E

E

S

S

I

P

G

R

F

F

L

F

I

L

K

E

A

N

G

G

R

K

T

H

A

D

E

E

A

A

binding (2S)-2-(2-oxidanylidenepyrrolidin-1-yl)butanamide: W164 (≠ Y154)

Sites not aligning to the query:

8jlcA Synaptic vesicle glycoprotein 2A (see paper)
30% identity, 45% coverage: 9:211/453 of query aligns to 19:234/592 of 8jlcA

query
sites
8jlcA

L

I

I

L

D

R

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

T

V

L

L

Y

F

F

W

V

T

L

A

G

L

L

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

K

E

D

W

M

H

C

L

L

D

S

P

D

I

S

Q

N

A

K

G

G

T

M

L

L

G

G

S

L

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

L

F

W

G

G

S

G

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

Q

V

C

I

L

A

L

V

I

C

S

V

L

V

S

L

V

F

N

S

S

L

V

F

F

T

A

V

F

L

F

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

T

F

F

A

L

I

F

C

C

R

R

F

L

V

L

A

S

G

G

L

V

G

G

I

I

G

G

V

S

M
x
I

P

P

N

I

A

V

V

F

A

S

L

Y

M

F

T

S

E

E

Y

F

A

L

P

A

K

Q

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F

C

S

M

G

F

Y
x
W

S

M

V

I

G

G

M

V

L

Y

S

A

A

A

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

S

-

F

-

Q

-

M

-

G

-

S

-

A

-

Y

-

Q

-

F

-

H

-

S

-

W

A

R

V

V

F

F

-

V

F

L

V

V

A

C

A

A

V

F

P

P

L

S

L

V

L

F

L

A

P

I

V

G

I

A

L

L

F

T

L

T

L

Q

P

P

E

E

S

S

I

P

G

R

F

F

L

F

I

L

K

E

A

N

G

G

R

K

T

H

A

D

E

E

A

A

binding (2S)-2-(2-oxidanylidenepyrrolidin-1-yl)butanamide: I137 (≠ M127), W164 (≠ Y154)

Sites not aligning to the query:

binding (2S)-2-(2-oxidanylidenepyrrolidin-1-yl)butanamide: 516, 520

8bvtA Cryo-em structure of rat slc22a6 bound to probenecid (see paper)
25% identity, 84% coverage: 63:441/453 of query aligns to 130:504/508 of 8bvtA

query
sites
8bvtA

S

S

Y

L

A

Y

L

M

F

V

G

G

M

V

M

L

F

L

G

G

A

A

L

M

T

V

F

F

G

G

S

Y

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

K

V

V

I

L

A

I

V

L

C

N

V

Y

V

L

L

Q

F

T

S

A

L

V

F

S

T

G

V

T

L

C

N

A

G

A

F

Y

V

A

R

P

D

N

T

Y

T

T

E

V

F

Y

A

C

I

V

C

F

R

R

F

L

V

L

A

S

G

G

L

M

G

S

I

L

G

A

G

S

V

I

M

A

P

I

N

N

A

C

V
x
M

A

T

L

L

M

N

T

V

E

E

Y

W

A

M

P

P

K

I

R

H

L

T

R

R

S

A

T

Y

L

V

V

G

A

T

V

L

M
x
I

F

G

S

Y

G

V

Y
|
Y

S

S

V

L

G

G

G

Q

M

F

L

L

S

L

A

A

G

G

L

I

G

A

M

Y

T

A

L

-

I

-

P

-

V

V

Y

P

G

H

W

W

S

R

A

H

V

L

F

Q

F

L

V

V

A

V

A

S

V

V

P

P

L

F

L

I

L

A

P

F

V

I

I

Y

L

S

F

W

L

F

P

I

E

E

S

S

I

A

G

R

F

W

L

Y

I

S

K

S

A

S

G

G

R

R

-

L

-

D

-

L

T

T

A

L

E

R

A

A

A

L

D

Q

K

R

L

V

A

A

R

R

L

I

E

N

P

G

S

-

Y

-

K

K

P

Q

Q

E

P

E

G

G

D

A

E

K

I

L

S

S

F

I

P

E

T

V

G

L

A

R

T

T

G

-

Q

-

A

-

S

S

L

L

P

Q

A

K

L

E

F

L

A

T

G

S

G

A

R

M

G

E

V

L

S

L

T

R

V

C

M

P

F

T

W

L

I

R

A

H

F

L

F

F

M

L

C

C

L

L

L

S

M

M

V

L

Y

W

A

F

L

A

G

T

S

S

-

F

-

A

W

Y

L
x
Y

P

G

K

L

L

V

M

M

N

D

N

L

A

Q

G

G

Y

F

G

G

L

V

G

S

S

M

S

Y

L

L

S

-

F

-

L

-

V

-

L

-

N

-

F

I

G

Q

A

V

I

I

F

F

G

G

A

A

V

V

G

D

-

L

-

P

-

A

-

K

-

F

-

V

G

C

G

F

H

L

I

V

A

I

D

N

R

S

M

M

N

G

L

R

K

R

R

P

V

A

L

Q

V

M

A

A

F

S

F

L

V

L

I

L

A

A

A

G

I

I

S

C

I

I

S

L

L

V

L

N

G

G

V

I

-

I

-

P

K

K

S

S

P

H

T

T

L

I

V

I

L

R

Y

T

V

S

L

L

V

A

A

V

V

L

A

G

G

K

A

G

T

C

T

L

I

A

G

S

T

S

Q

F

I

N

L

C

L

I

Y

F

A

L

Y

Y

V

T

A

G

Q

E

F

L

Y

Y

P

P

M

T

A

V

I

I

R

R

S

Q

T

T

G

G

I

L

G

G

W

M

A

G

S

S

G

T

V

M

G

A

R

R

S

V

G

G

A

S

I

I

L

V

G

S

P

P

V

L

L

V

G

S

G

M

T

T

L

-

A

A

W

E

K

F

L

Y

P

P

L

S

M

M

Q

P

N

L

F

F

M

I

A

F

F

G

A

A

V

V

P

P

G

V

V

V

I

A

A

S

A

A

I

V

A

T

M

A

S

L

L

L

V

P

V

E

R

T

G

L

A

G

Q

Q

H

P

A

L

P

P

E

D

E

T

E

V

R

Q

D

D

L

L

binding 4-(dipropylsulfamoyl)benzoic acid: M198 (≠ V131), I217 (≠ M150), Y221 (= Y154), Y339 (≠ L275)

Sites not aligning to the query:

binding 4-(dipropylsulfamoyl)benzoic acid: 31, 35

8bvsA Cryo-em structure of rat slc22a6 bound to tenofovir (see paper)
25% identity, 84% coverage: 63:441/453 of query aligns to 121:495/502 of 8bvsA

query
sites
8bvsA

S

S

Y

L

A

Y

L

M

F

V

G

G

M

V

M

L

F

L

G

G

A

A

L

M

T

V

F

F

G

G

S

Y

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

K

V

V

I

L

A

I

V

L

C

N

V

Y

V

L

L

Q

F

T

S

A

L

V

F

S

T

G

V

T

L

C

N

A

G

A

F

Y

V

A

R

P

D

N

T

Y

T

T

E

V

F

Y

A

C

I

V

C

F

R

R

F

L

V

L

A

S

G

G

L

M

G

S

I

L

G

A

G

S

V

I

M

A

P

I

N

N

A

C

V

M

A

T

L

L

M

N

T

V

E

E

Y

W

A

M

P

P

K

I

R

H

L

T

R

R

S

A

T

Y

L

V

V

G

A

T

V

L

M

I

F

G

S

Y

G

V

Y
|
Y

S

S

V

L

G

G

G

Q

M

F

L

L

S

L

A

A

G

G

L

I

G

A

M

Y

T

A

L

-

I

-

P

-

V

V

Y

P

G

H

W

W

S

R

A

H

V

L

F

Q

F

L

V

V

A

V

A

S

V

V

P

P

L

F

L

I

L

A

P

F

V

I

I

Y

L

S

F

W

L

F

P

I

E

E

S

S

I

A

G

R

F

W

L

Y

I

S

K

S

A

S

G

G

R

R

-

L

-

D

-

L

T

T

A

L

E

R

A

A

A

L

D

Q

K

R

L

V

A

A

R

R

L

I

E

N

P

G

S

-

Y

-

K

K

P

Q

Q

E

P

E

G

G

D

A

E

K

I

L

S

S

F

I

P

E

T

V

G

L

A

R

T

T

G

-

Q

-

A

-

S

S

L

L

P

Q

A

K

L

E

F

L

A

T

G

S

G

A

R

M

G

E

V

L

S

L

T

R

V

C

M

P

F

T

W

L

I

R

A

H

F

L

F

F

M

L

C

C

L

L

L

S

M

M

V

L

Y

W

A

F

L

A

G

T

S

S

-

F

-

A

W

Y

L
x
Y

P

G

K

L

L

V

M

M

N

D

N

L

A

Q

G

G

Y

F

G

G

L

V

G

S

S

M

S

Y

L

L

S

-

F

-

L

-

V

-

L

-

N

-

F

I

G

Q

A

V

I

I

F

F

G

G

A

A

V

V

G

D

-

L

-

P

-

A

-

K

-

F

-

V

G

C

G

F

H

L

I

V

A

I

D

N

R

S

M

M

N

G

L

R

K

R

R

P

V

A

L

Q

V

M

A

A

F

S

F

L

V

L

I

L

A

A

A

G

I

I

S

C

I

I

S

L

L

V

L

N

G

G

V

I

-

I

-

P

K

K

S

S

P

H

T

T

L

I

V

I

L

R

Y

T

V

S

L

L

V

A

A

V

V

L

A

G

G

K

A

G

T

C

T

L

I

A

G

S

T

S

Q

F

I

N

L

C

L

I

Y

F

A

L

Y

Y

V

T

A

G

Q

E

F

L

Y

Y

P

P

M

T

A

V

I

I

R

R

S

Q

T

T

G

G

I

L

G

G

W

M

A

G

S

S

G

T

V

M

G

A

R

R

S

V

G

G

A

S

I

I

L

V

G

S

P

P

V

L

L

V

G

S

G

M

T

T

L

-

A

A

W

E

K

F

L

Y

P

P

L

S

M

M

Q

P

N

L

F

F

M

I

A

F

F

G

A

A

V

V

P

P

G

V

V

V

I

A

A

S

A

A

I

V

A

T

M

A

S

L

L

L

V

P

V

E

R

T

G

L

A

G

Q

Q

H

P

A

L

P

P

E

D

E

T

E

V

R

Q

D

D

L

L

binding [2-(6-amino-9h-purin-9-yl)-1-methylethoxy]methylphosphonic acid: Y212 (= Y154), Y330 (≠ L275)

8bw7A Cryo-em structure of rat slc22a6 bound to alpha-ketoglutaric acid (see paper)
25% identity, 84% coverage: 63:441/453 of query aligns to 121:491/497 of 8bw7A

query
sites
8bw7A

S

S

Y

L

A

Y

L

M

F

V

G

G

M

V

M

L

F

L

G

G

A

A

L

M

T

V

F

F

G

G

S

Y

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

K

V

V

I

L

A

I

V

L

C

N

V

Y

V

L

L

Q

F

T

S

A

L

V

F

S

T

G

V

T

L

C

N

A

G

A

F

Y

V

A

R

P

D

N

T

Y

T

T

E

V

F

Y

A

C

I

V

C

F

R

R

F

L

V

L

A

S

G

G

L

M

G

S

I

L

G

A

G

S

V

I

M

A

P

I

N

N

A

C

V

M

A

T

L

L

M

N

T

V

E

E

Y

W

A

M

P

P

K

I

R

H

L

T

R

R

S

A

T

Y

L

V

V

G

A

T

V

L

M

I

F
x
G

S

Y

G

V

Y
|
Y

S

S

V

L

G

G

G

Q

M

F

L

L

S

L

A

A

G

G

L

I

G

A

M

Y

T

A

L

-

I

-

P

-

V

V

Y

P

G

H

W

W

S

R

A

H

V

L

F

Q

F

L

V

V

A

V

A

S

V

V

P

P

L

F

L

I

L

A

P

F

V

I

I

Y

L

S

F

W

L

F

P

I

E

E

S

S

I

A

G

R

F

W

L

Y

I

S

K

S

A

S

G

G

R

R

-

L

-

D

-

L

T

T

A

L

E

R

A

A

A

L

D

Q

K

R

L

V

A

A

R

R

L

I

E

N

P

G

S

-

Y

-

K

K

P

Q

Q

E

P

E

G

G

D

A

E

K

I

L

S

S

F

I

P

E

T

V

G

L

A

R

T

T

G

S

Q

L

A

Q

S

S

L

A

P

M

A

E

L

L

F

L

A

R

G

-

R

-

G

-

V

C

S

P

T

T

V

L

M

R

F

H

W

-

I

-

A

-

F

L

F

F

M

L

C

C

L

L

L

S

M

M

V

L

Y

W

A

F

L

A

G

T

S

S

-

F

-

A

W

Y

L

Y

P

G

K

L

L

V

M

M

N

D

N

L

A

Q

G

G

Y

F

G

G

L

V

G

S

S

M

S

Y

L

L

S

-

F

-

L

-

V

-

L

-

N

-

F

I

G

Q

A

V

I

I

F

F

G

G

A

A

V

V

G

D

-

L

-

P

-

A

-
x
K

-

F

-

V

G

C

G

F

H

L

I

V

A

I

D

N

R

S

M

M

N

G

L

R

K

R

R

P

V

A

L

Q

V

M

A

A

F

S

F

L

V

L

I

L

A

A

A

G

I

I

S

C

I

I

S

L

L

V

L

N

G

G

V

I

-

I

-

P

K

K

S

S

P

H

T

T

L

I

V

I

L

R

Y

T

V

S

L

L

V

A

A

V

V

L

A

G

G

K

A

G

T

C

T

L

I

A

G

S

T

S

Q

F

I

N

L

C

L

I

Y

F

A

L

Y

Y

V

T

A

G

Q

E

F

L

Y

Y

P

P

M

T

A

V

I

I

R

R

S

Q

T

T

G

G

I

L

G

G

W

M

A

G

S

S

G

T

V

M

G

A

R

R

S

V

G

G

A

S

I

I

L

V

G

S

P

P

V

L

L

V

G

S

G

M

T

T

L

-

A

A

W

E

K

F

L

Y

P

P

L

S

M

M

Q

P

N

L

F

F

M

I

A

F

F

G

A

A

V

V

P

P

G

V

V

V

I

A

A

S

A

A

I

V

A

T

M

A

S

L

L

L

V

P

V

E

R

T

G

L

A

G

Q

Q

H

P

A

L

P

P

E

D

E

T

E

V

R

Q

D

D

L

L

binding 2-oxoglutaric acid: G209 (≠ F151), Y212 (= Y154), K354 (vs. gap)

8uo9A Structure of synaptic vesicle protein 2a in complex with a nanobody (see paper)
29% identity, 45% coverage: 9:211/453 of query aligns to 12:218/555 of 8uo9A

query
sites
8uo9A

L

I

I

L

D

R

E

E

A

C

K

G

F

H

G

G

K

R

F

F

H

Q

W

W

N

T

V

L

L

Y

F

F

W

V

T

L

A

G

L

L

I

A

I

L

I

M

F

A

D

D

G

G

Y

V

D

E

L

V

V

F

I

V

Y

V

G

G

V

F

V

V

L

L

P

P

V

S

L

A

M

E

K

K

E

D

W

-

H

-

L

-

D

-

P

-

I

-

Q

-

A

-

G

-

T

M

L

L

G

G

S

L

Y

I

A

V

L

Y

F

L

G

G

M

M

F

V

G

G

A

A

L

F

T

L

F

W

G

G

S

G

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

Q

V

C

I

L

A

L

V

I

C

S

V

L

V

S

L

V

F

N

S

S

L

V

F

F

T

A

V

F

L

F

N

S

G

S

F

F

V

V

R

Q

D

G

T

Y

T

G

E

T

F

F

A

L

I

F

C

C

R

R

F

L

V

L

A

S

G

G

L

V

G

G

I

I

G

G

V

S

M

I

P

P

N

I

A

V

V

F

A

S

L

Y

M

F

T

S

E

E

Y

F

A

L

P

A

K

Q

R

E

L

K

R

R

S

G

T

E

L

H

V

L

A

S

V

W

M

L

F
x
C

S

M

G

F

Y
x
W

S

M

V

I

G

G

M

V

L

Y

S

A

A

A

G

A

L

M

G

A

M

W

T

A

L

I

I

I

P

P

V

H

Y

Y

G

G

W

W

S

S

-

F

-

Q

-

M

-

G

-

S

-

A

-

Y

-

Q

-

F

-

H

-

S

-

W

A

R

V

V

F

F

-

V

F

L

V

V

A

C

A

A

V

F

P

P

L

S

L

V

L

F

L

A

P

I

V

G

I

A

L

L

F

T

L

T

L

Q

P

P

E

E

S

S

I

P

G

R

F

F

L

F

I

L

K

E

A

N

G

G

R

K

T

H

A

D

E

E

A

A

binding (4R)-1-{[(4S)-2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl}-4-(4,4,4-trifluorobutyl)pyrrolidin-2-one: C145 (≠ F151), W148 (≠ Y154)

Sites not aligning to the query:

Q4U2R8 Solute carrier family 22 member 6; Organic anion transporter 1; hOAT1; PAH transporter; hPAHT; Renal organic anion transporter 1; hROAT1 from Homo sapiens (Human) (see 6 papers)
24% identity, 84% coverage: 63:441/453 of query aligns to 139:519/563 of Q4U2R8

query
sites
Q4U2R8

S

S

Y

L

A

Y

L

M

F

V

G

G

M

V

M

L

F

L

G

G

A

A

L

M

T

V

F

F

G

G

S

Y

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

K

V

V

I

L

A

I

V

L

C

N

V

Y

V

L

L

Q

F

T

S

A

L

V

F

S

T

G

V

T

L

C

N

A

G

A

F

F

V

A

R

P

D

N

T

F

T

P

E

I

F

Y

A

C

I

A

C

F

R

R

F

L

V

L

A

S

G

G

L

M

G

A

I

L

G

A

G

G

V

I

M

S

P

L

N

N

A

C

V

M

A

T

L

L

M

N

T

V

E

E

Y

W

A

M

P

P

K

I

R

H

L

T

R

R

S

A

T

C

L

V

V

G

A

T

V

L

M

I

F

G

S

Y

G

V

Y
|
Y

S

S

V

L

G

G

G

Q

M

F

L

L

S

L

A

A

G

G

L

V

G

A

M

Y

T

A

L

-

I

-

P

-

V

V

Y

P

G

H

W

W

S

R

A

H

V

L

F

Q

F

L

V

L

A

V

A

S

V

A

P

P

L

F

L

A

L

F

P

F

V

I

I

Y

L

S

F

W

L

F

P

I

E

E

S

S

I

A

G

R

F

W

L

H

I

S

K

S

A

S

G

G

R

R

-

L

-

D

-

L

T

T

A

L

E

R

A

A

A

L

D

Q

K

R

L

V

A

A

R

R

L

I

E

-

P

-

S

N

Y

G

K

K

P

R

Q

E

P

E

G

G

D

A

E

K

I

L

S

S

F

M

P

-

T

-

G

-

A

E

T

V

G

L

Q

R

A

A

S

S

L

L

P

Q

A

K

L

E

F

L

A

T

G

M

G

G

R

K

G

G

V

Q

S

A

T

S

V

A

M

M

F

E

W

L

I

L

A

R

-

C

-

P

-

T

-

L

-

R

-

H

F

L

F

F

M

L

C

C

L

L

L

S

M

M

V

L

Y

W

A

F

L

A

G

T

S

S

-

F

-

A

W

Y

L

Y

P

G

K

L

L

V

M

M

N

D

N

L

A

Q

G

G

Y

F

G

G

L

V

G

S

S

-

L

-

S

I

F

Y

L

L

L

I

V

Q

L

V

N

I

F

F

G

G

A

A

I

V

F

D

-

L

-

P

G

A

A

K

V

L

G

V

G

G

G

F

H

L

I

V

A

I

D

N

R

S

M

L

N

G

L

R

K

R

R

P

V

A

L

Q

V

M

A

A

F

A

F

L

V

L

I

L

A

A

A

G

I

I

S

C

I

I

S

L

L

L

L

N

G

G

V

V

-

I

-

P

K

Q

S

D

P

Q

T

S

L

I

V

V

L

R

Y

T

V

S

L

L

V

A

A

V

V

L

A

G

G
x
K

A

G

T

C

T

L

I

A

G

A

T

S

Q
x
F

I

N

L

C

L

I

Y

F

A

L

Y

Y

V

T

A

G

Q

E

F

L

Y

Y

P

P

M

T

A

M

I

I

R

R

S

Q

T

T

G

G

I

M

G

G

W

M

A

G

S

S

G

T

V

M

G

A

R

R

S

V

G

G

A

S

I

I

L

V

G

S

P

P

V

L

L

V

G

-

T

S

L

M

L

T

A

A

W

E

K

L

L

Y

P

P

L

S

M

M

Q

P

N

L

F

F

M

I

A

Y

F

G

A

A

V

V

P

P

G

V

V

A

I

A

A

S

A

A

I

V

A

T

M

V

S

L

L

L

V

P

V

E

R

T

G

L

A

G

Q

Q

H

P

A

L

P

P

E

D

E

T

E

V

R

Q

D

D

L

L

Y230 (= Y154) mutation to A: Loss of membrane protein expression and little uptake of cidofovir.
K431 (≠ G351) mutation to A: Decrease in the level of membrane protein expression and 70 % loss of PAH uptake.
F438 (≠ Q358) mutation to A: Decrease in the level of membrane protein expression, 70 % loss of PAH uptake, increased affinity for cidofovir, lower Vmax for PAH, and lower Km and Vmax for cidofovir.

Sites not aligning to the query:

7 L → P: in dbSNP:rs1415632329
30 L→A: Complete loss of PAH transport activity.
36 T→A: Complete loss of PAH transport activity.
39 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Complete loss of PAH transport activity.
50 R → H: lower Vmax; increase in substrate affinity and increase in the affinity for the nucleoside phosphonate analogs cidofovir, adefovir and tenofovir; dbSNP:rs11568626
56 modified: carbohydrate, N-linked (GlcNAc...) asparagine
92 modified: carbohydrate, N-linked (GlcNAc...) asparagine
97 modified: carbohydrate, N-linked (GlcNAc...) asparagine
104 P → L: in dbSNP:rs11568627
113 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q8VC69 Solute carrier family 22 member 6; Kidney-specific transport protein; Novel kidney transcript; mNKT; Organic anion transporter 1; mOAT1; Renal organic anion transporter 1; mROAT1 from Mus musculus (Mouse) (see 2 papers)
24% identity, 83% coverage: 66:441/453 of query aligns to 136:513/545 of Q8VC69

query
sites
Q8VC69

L

M

F

V

G

G

M

V

M

L

F

L

G

G

A

A

L

M

T

M

F

F

G

G

S

Y

L

L

S

A

D

D

R

R

F

L

G

G

R

R

K

R

P

K

V

V

I

L

A

I

V

L

C

N

V

Y

V

L

L

Q

F

T

S

A

L

V

F

S

T

G

V

T

L

C

N

A

G

A

F

Y

V

A

R

P

D

N

T

Y

T

T

E

V

F

Y

A
x
C

I

I

C

F

R

R

F

L

V

L

A

S

G

G

L

M

G

S

I

L

G

A

G

S

V

I

M

A

P

I

N

N

A

C

V

M

A

T

L

L

M

N

T

M

E

E

Y

W

A

M

P

P

K

I

R

H

L

T

R

R

S

A

T

Y

L

V

V

G

A

T

V

L

M

I

F

G

S

Y

G

V

Y

Y

S

S

V

L

G

G

G

Q

M

F

L

L

S

L

A

A

G

G

L

I

G

A

M

Y

T

A

L

-

I

-

P

-

V

V

Y

P

G

H

W

W

S

R

A

H

V

L

F

Q

F

L

V

A

A

V

A

S

V

V

P

P

L

F

L

V

L

A

P

F

V

I

I

Y

L

S

F

W

L

F

P

I

E

E

S

S

I

A

G

R

F

W

L

Y

I

S

K

S

A

S

G

G

R

R

-

L

-

D

-

L

T

T

A

L

E

R

A

A

A

L

D

Q

K

R

L

V

A

A

R

R

L

I

E

N

P

G

S

-

Y

-

K

K

P

Q

Q

E

P

E

G

G

D

A

E

K

I

L

S

S

F

I

P

-

T

-

G

-

A

E

T

V

G

L

Q

Q

A

T

S

S

L

L

P

Q

A

K

L

E

F

L

A

T

G

L

G

N

R

K

G

G

V

Q

S

A

T

S

V

A

M

M

F

E

W

L

I

L

-

R

-

C

-

P

-

T

-

L

-

R

A

R

F

L

F

F

M

L

C

C

L

L

L

S

M

M

V

L

Y

W

A

F

L

A

G

T

S

S

-

F

-

A

W

Y

L

Y

P

G

K

L

L

V

M

M

N

D

N

L

A

Q

G

G

Y

F

G

G

L

V

G

S

S

M

S

Y

L

L

S

-

F

-

L

-

V

-

L

-

N

-

F

I

G

Q

A

V

I

I

F

F

G

G

A

A

V

V

G

D

-

L

-

P

-

A

-

K

-

F

-

V

G

C

G

F

H

L

I

V

A

I

D

N

R

S

M

M

N

G

L

R

K

R

R

P

V

A

L

Q

V

L

A

A

F

S

F

L

V

L

I

L

A

A

A

G

I

I

S

C

I

I

S

L

L

V

L

N

G

G

V

I

-

I

-

P

K

R

S

G

P

H

T

T

L

I

V

I

L

R

Y

T

V

S

L

L

V

A

A

V

V

L

A

G

G

K

A

G

T

C

T

L

I

A

G

S

T

S

Q

F

I

N

L
x
C

L

I

Y

F

A

L

Y

Y

V

T

A

G

Q

E

F

L

Y

Y

P

P

M

T

A

M

I

I

R

R

S

Q

T

T

G

G

I

L

G

G

W

M

A

G

S

S

G

T

V

M

G

A

R

R

S

V

G

G

A

S

I

I

L

V

G

S

P

P

V

L

L

I

G

S

G

M

T

T

L

-

A

A

W

E

K

F

L

Y

P

P

L

S

M

I

Q

P

N

L

F

F

M

I

A

F

F

G

A

A

V

V

P

P

G

V

V

A

I

A

A

S

A

A

I

V

A

T

M

A

S

L

L

L

V

P

V

E

R

T

G

L

A

G

Q

Q

H

P

A

L

P

P

E

D

E

T

E

V

R

Q

D

D

L

L

C183 (≠ A113) mutation to A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-122; A-172; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
C434 (≠ L360) mutation to A: Decreased cell surface expression level and PAH transport activity. 80% decrease of PAH transport activity; when associated with A-49; A-122 and A-183. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402 and A-427.

Sites not aligning to the query:

39 N→Q: Complete loss of PAH transport activity.
49 C→A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
56 modified: carbohydrate, N-linked (GlcNAc...) asparagine
86 modified: carbohydrate, N-linked (GlcNAc...) asparagine
91 modified: carbohydrate, N-linked (GlcNAc...) asparagine
107 modified: carbohydrate, N-linked (GlcNAc...) asparagine
122 C→A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.

Q9VCA2 Organic cation transporter protein from Drosophila melanogaster (Fruit fly) (see paper)
27% identity, 79% coverage: 50:407/453 of query aligns to 119:487/548 of Q9VCA2

query
sites
Q9VCA2

E

E

W

W

H

N

L

L

-

V

-

C

-

S

D

R

P

S

I

L

Q

L

A

S

G

A

T

T

L

S

G

D

S

S

Y

L

A

F

L

M

F

L

G

G

M

V

M

L

F

L

G

G

A

S

L

L

T

I

F

F

G

G

S

Q

L

M

S

S

D

D

R

K

F

L

G

G

R

R

K

K

P

P

V

T

I

F

A

F

V

A

C

S

V

L

V

V

L

L

F

Q

S

L

L

I

F

F

T

G

V

V

L

L

N

A

G

A

F

V

V

A

R

P

D

E

T

Y

T

F

E

S

F

Y

A

T

I

I

C

S

R

R

F

M

V

I

A

V

G

G

L

A

G

T

I

T

G

S

G

G

V

V

M

F

P

L

N

V

A

A

V

Y

A

V

L

I

M

A

T

L

E

E

Y

M

-

V

-

G

A

S

P

S

K

Y

R

R

L

L

R

F

S

A

T

G

L

V

V

A

A

M

V

Q

M

M

F

F

S

-

G

-

Y

F

S

S

V

V

G

G

G

F

M

M

L

L

S

T

A

A

G

G

L

F

G

A

M

Y

T

F

L

-

I

-

P

-

V

I

Y

H

G

D

W

W

S

R

A

W

V

L

F

Q

F

I

V

A

A

I

A

T

V

L

P

P

L

G

L

L

L

F

P

L

V

C

I

Y

L

Y

F

W

L

I

P

P

E

E

S

S

I

A

G

R

F

W

L

L

I

L

K

M

A

K

G

G

R

R

T

K

A

D

E

E

A

A

-

F

-

V

-

I

A

I

D

E

K

K

L

A

A

A

R

K

L

E

E

N

P

K

S

V

Y

E

K

V

P

P

-

N

-

E

-

I

-

Y

-

E

Q

Q

P

L

G

V

D

D

E

E

I

V

S

A

F

E

P

K

T

K

G

K

A

Q

T

D

G

E

Q

M

-

A

A

A

S

S

L

Q

P

P

A

A

-

A

-

T

-

V

-

F

-

D

-

L

L

L

F

R

A

Y

G

P

G

N

R

L

G

R

V

R

S

K

T

T

V

L

M

L

F

I

W

F

I

F

A

D

F

W

F

F

M

V

C

N

L

S

L

G

M

V

V

Y

Y

Y

A

G

L

L

G

-

S

S

W

W

L

-

P

-

K

-

L

N

M

T

N

N

A

L

G

G

Y

-

G

-

L

-

G

G

S

N

S

Q

L

L

S

V

F

N

L

F

L

M

V

I

L

S

N

G

F

A

G

V

A

E

I

I

F

P

G

G

A

Y

V

T

G

L

H

F

I

T

A

L

D

N

R

R

M

W

N

G

L

R

K

R

R

S

V

I

L

L

V

C

A

G

F

T

F

M

V

M

I

V

A

A

A

G

I

I

S

S

I

L

S

-

L

L

L

A

G

T

V

I

K

F

S

V

P

P

T

S

L

D

V

M

L

N

Y

W

V

L

L

I

V

V

A

A

V

C

A

A

-

M

-

I

G

G

A

K

T

L

T

A

I

I

G

T

T

S

Q

S

I

Y

-

G

L

T

L

I

Y

Y

A

I

Y

F

V

S

A

A

Q

E

F

Q

Y

F

P

P

M

T

A

V

I

V

R

R

S

N

T

V

G

G

I

L

G

G

W

A

A

S

S

S

G

M

V

V

G

A

R

R

S

V

G

G

A

G

I

I

L

L

G

A

P

P

V

Y

L

L

G

K

G

L

T

L

L

G

L

E

A

I

W

W

K

R

-

P

L

L

P

P

L

L

M

I

Sites not aligning to the query:

97 modified: carbohydrate, N-linked (GlcNAc...) asparagine

O57379 Solute carrier family 22 member 6; Organic anion transporter 1; Renal organic anion transporter 1; ROAT1; fROAT1 from Pseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus) (see paper)
25% identity, 77% coverage: 46:395/453 of query aligns to 131:487/562 of O57379

query
sites
O57379

V

T

L

I

M

I

K

S

E

E

W

W

H

H

L

L

-

V

-

C

D

D

P

M

I

H

Q

S

A

F

G

K

T

Q

L

M

G

G

S

Q

Y

T

A

I

L

Y

F

M

G

G

-

G

M

V

M

L

F

V

G

G

A

A

L

L

T

L

F

F

G

G

S

G

L

L

S

S

D

D

R

R

F

Y

G

G

R

R

K

R

P

I

V

L

I

L

A

L

V

I

C

S

V

N

V

L

L

L

F

M

S

A

L

V

F

S

T

G

V

T

L

C

N

A

G

A

F

F

V

S

R

S

D

S

T

F

T

S

E

L

F

F

A

C

I

V

C

F

R

R

F

F

V

G

A

C

G

G

L

L

G

A

I

L

G

S

G

G

V

L

M

G

P

L

N

N

A

T

V

F

A

S

L

L

M

I

T

V

E

E

Y

W

A

I

P

P

K

T

R

R

L

I

R

R

S

T

T

A

L

V

V

G

A

T

V

T

M

T

F

G

S

Y

G

C

Y

Y

S

T

V

L

G

G

G

Q

M

L

L

I

S

-

A

-

G

-

L

L

G

V

M

L

T

L

L

A

I

Y

P

F

V

I

Y

R

G

D

W

W

S

R

A

W

V

L

F

T

F

L

V

A

A

V

A

S

V

L

P

P

L

F

L

Y

L

V

L

F

P

F

V

L

I

I

L

A

F

W

L

W

L

F

P

H

E

E

S

S

I

S

G

R

F

W

L

L

I

A

K

L

A

S

G

N

R

R

T

T

A

E

E

H

A

A

A

L

D

K

K

N

L

L

-

K

-

S

-

V

A

A

R

R

L

F

E

N

P

G

S

R

Y

H

K

E

P

E

Q

A

P

E

G

K

D

L

E

D

I

I

S

K

F

M

P

L

T

-

G

-

A

-

T

-

G

-

Q

H

A

E

S

S

L

M

P

K

A

K

L

E

F

M

A

S

G

C

G

T

R

Q

G

G

V

S

S

Y

T

S

V

I

M

L

F

D

W

L

I

F

-

N

-

T

-

P

-

A

-

M

-

R

A

K

F

R

F

T

M

L

C

C

L

L

L

S

M

A

V

V

Y

W

A

L

L

S

G

T

S

S

W

F

L

-

P

-

K

-

L

-

M

-

N

-

A

A

G

Y

Y

Y

G

G

L

L

G

A

S

M

S

D

L

L

S

D

F

K

L

F

L

G

V

V

L

D

N

I

F

Y

-

L

-

I

G

Q

A

V

I

I

F

F

G

G

A

A

V

V

-

D

-

I

-

P

G

A

G
x
K

G

V

H

V

I

V

A

V

D

V

R

S

M

M

N

S

L

L

-

I

-

G

-

R

K

R

R

R

V

S

L

Q

V

C

A

A

F

V

F

L

V

V

I

V

A

A

A

G

I

I

S

T

I

I

S

L

L

L

-

N

L

L

G

L

V

V

K

P

S

Y

P

D

T

K

L

Q

V

T

L

I

Y

R

V

T

L

C

V

L

A

A

V

V

A

L

G

G

A

K

T

G

T

C

I

L

G

A

T

A

Q

S

I

F

-

N

L

C

Y

Y

A

L

Y

Y

V

S

A

G

Q

E

F

L

Y

F

P

P

M

T

A

I

I

I

R

R

S

Q

T

N

G

G

I

M

G

G

W

W

A

V

S

S

G

M

V

M

G

A

R
|
R

S

I

G

G

A

A

I

M

L

V

G

A

P

P

V

M

L

V

K394 (≠ G307) mutation to A: Reduced transport activity.
R478 (= R386) mutation to D: Reduced transport activity.

Sites not aligning to the query:

34 H→I: Reduced transport activity.

Q9NSA0 Solute carrier family 22 member 11; Organic anion transporter 4; OAT4; Organic anion:dicarboxylate exchanger OAT4 from Homo sapiens (Human) (see 3 papers)
25% identity, 72% coverage: 68:395/453 of query aligns to 152:482/550 of Q9NSA0

query
sites
Q9NSA0

G

G

M

I

M

L

F

V

G

G

A

S

L

F

T

I

F

W

G

G

S

L

L

L

S

S

D

Y

R

R

F

F

G

G

R

R

K

K

P

P

V

M

I

L

A

S

V

W

C

C

V

C

V

L

L

Q

F

L

S

A

L

V

F

A

T

G

V

T

L

S

N

T

G

I

F

F

V

A

R

P

D

T

T

F

T

V

E

I

F

Y

A

C

I

G

C

L

R

R

F

F

V

V

A

A

G

A

L

F

G

G

I

M

G

A

G

G

V

I

M

F

P

L

N

S

A

S

V

L

A

T

L

L

M

M

T

V

E

E

Y

W

A

T

P

T

K

T

R

S

L

R

R

R

S

A

T

V

L

T

V

M

A

T

V

V

M

V

F

G

S

C

G

A

Y

F

S

S

V

A

G
|
G

G

-

M

-

L

-

S

Q

A

A

G

A

L

L

G

G

M

G

T

L

L

A

I

F

P

A

V

L

Y

R

G

D

W

W

S

R

A

T

V

L

F

Q

F

L

V

A

A

A

A

S

V

V

P

P

L

F

L

A

L

I

P

S

V

L

I

I

L

S

F

W

L

W

L

L

P

P

E

E

S

S

I

A

G

R

F

W

L

L

I

I

K

I

A

K

G

G

R

K

T

P

A

D

E

Q

A

A

A

L

D

Q

K

E

L

L

A

R

R

K

L

V

E

A

P

R

S

I

Y

N

K

G

P
x
H

Q

K

P

E

G

A

D

K

E

N

I

L

S

T

F

I

P

E

T

V

G

L

A

M

T

S

G

S

Q

V

A

K

S

E

L

E

P

V

A

A

L

S

F

A

A

K

G

E

G

P

R

R

G

S

V

V

S

L

T

D

-

L

-

F

-

C

-

V

-

P

V

V

M

L

F

R

W

W

I

R

A

S

F

C

F

A

M

M

C

L

L

V

M

N

V

F

Y

S

A

L

L

L

G

I

S

S

W

Y

L

-

P

-

K

-

L

-

M

-

N

-

A

-

G

-

Y

Y

G

G

L

L

G

V

S

F

S

D

L

L

S

Q

-

S

-

L

-

G

-

R

-

D

-

I

F

F

L

L

V

Q

L

A

N

L

F

F

G

G

A

A

I

V

-

D

-

F

F

L

G

G

A

R

V

A

G

T

G

A

H

L

I

L

A

L

D

S

R

F

M

L

N
x
G

L

R

K

R

R

T

V

I

L

Q

V

A

A

G

F

S

F

Q

V

A

I

M

A

A

A

G

I

L

S

A

I

I

-

L

S

A

L

N

L

M

G

L

V

V

K

P

S

Q

P

D

T

L

L

Q

V

T

L

L

Y

R

V

V

L

V

V

F

A

A

V

V

A

L

G

G

A

K

T

G

T

C

I

F

G

G

T

I

Q

S

I

L

-

T

L

C

L

L

Y

T

A

I

Y

Y

V

K

A

A

Q

E

F

L

Y

F

P

P

M

T

A

P

I

V

R

R

S

M

T

T

G

A

I

D

G

G

W

I

A

L

S
x
H

G

T

V

V

G

G

R

R

S

L

G

G

A

A

I

M

L

M

G

G

P

P

V

L

L

I

G241 (= G157) mutation G->L,S,V: Strongly reduced cell surface expression and estrone 3-sulfate transport.
H305 (≠ P224) mutation to A: Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-52; A-83 and A-469.
G400 (≠ N315) mutation G->L,S,V: Strongly reduced cell surface expression and estrone 3-sulfate transport.
H469 (≠ S382) mutation to A: Slightly reduced estrone 3-sulfate transport. Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-52; A-83 and A-305.

Sites not aligning to the query:

39 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface location; when associated with Q-56; Q-63 and Q-99.
47 H→A: Reduced cell surface expression and estrone 3-sulfate transport. Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-52; A-83; A-305 and A-469.
52 H→A: Slightly reduced estrone 3-sulfate transport. Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-83; A-305 and A-469.
56 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-63 and Q-99.
63 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-56 and Q-99.
83 H→A: Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-52; A-305 and A-469.
99 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-56 and Q-63.

Query Sequence

>WP_011386821.1 NCBI__GCF_000009985.1:WP_011386821.1
MALVNAHKLIDEAKFGKFHWNVLFWTALIIIFDGYDLVIYGVVLPVLMKEWHLDPIQAGT
LGSYALFGMMFGALTFGSLSDRFGRKPVIAVCVVLFSLFTVLNGFVRDTTEFAICRFVAG
LGIGGVMPNAVALMTEYAPKRLRSTLVAVMFSGYSVGGMLSAGLGMTLIPVYGWSAVFFV
AAVPLLLLPVILFLLPESIGFLIKAGRTAEAADKLARLEPSYKPQPGDEISFPTGATGQA
SLPALFAGGRGVSTVMFWIAFFMCLLMVYALGSWLPKLMNNAGYGLGSSLSFLLVLNFGA
IFGAVGGGHIADRMNLKRVLVAFFVIAAISISLLGVKSPTLVLYVLVAVAGATTIGTQIL
LYAYVAQFYPMAIRSTGIGWASGVGRSGAILGPVLGGTLLAWKLPLMQNFMAFAVPGVIA
AIAMSLVVRGAQHAPEEERDLAFAAETGMMEPD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory