SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011389007.1 NCBI__GCF_000013085.1:WP_011389007.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
34% identity, 96% coverage: 17:447/450 of query aligns to 40:469/472 of P78061

query
sites
P78061

V

V

E

D

C

V

L

L

S

T

D

D

M

L

N

N

G

G

I

C

V

F

R

R

G

G

K

K

V

R

L

I

P

P

A

V

R

S

K

S

F

-

L

L

R

K

S

K

L

L

E

E

D

K

N

G

S

C

L

Y

R

-

I

F

P

P

S

A

S

S

V

V

F

F

L

A

V

M

T

D

V

I

T

L

G

G

E

N

Y

V

P

V

F

E

A

E

D

A

D

G

N

L

N

G

T

Q

V

E

V

M

I

G

D

E

P

P

D

D

A

-

D

-

L

-

R

-

P

-

D

-

F

-

R

R

T

T

L

C

C

V

V

P

A

V

P

L

G

G

Y

S

R

L

T

T

P

P

T

S

A

A

F

A

-

D

-

P

-

E

-

F

-

I

-

G

-

Q

V

M

V

L

D

T

A

M

Y

V

K

D

S

E

N

D

G

G

I

A

P

P

L

F

D

D

L

V

S

E

P

P

R

R

Q

N

V

V

L

L

K

N

R

R

V

L

L

W

A

Q

L

Q

Y

L

S

R

E

Q

Q

R

G

G

W

L

K

F

P

P

V

V

A

A

P

V

E

E

I

L

E

E

F

F

Y

Y

L

L

T

L

Q

D

V

R

N

Q

A

R

D

D

P

A

D

E

L

G

P

Y

L

L

S

Q

P

P

A

C

-

A

-

P

G

G

R

T

S

D

G

D

R

R

P

-

E

N

T

T

S

Q

P

S

Q

Q

P

V

Y

Y

G

S

L

V

E

D

A

N

I

L

T

N

E

H

F

F

E

A

D

D

L

V

I

L

E

N

N

D

I

I

Y

D

E

E

H

L

A

A

E

Q

I

L

A

Q

G

L

L

I

Q

P

I

A

D

D

T

G

L

A

I

V

H

A

E

E

A

A

G

S

A

P

A

G

Q

Q

L

F

E

E

I

I

N

N

F

L

L

Y

H

H

G

T

D

D

P

N

I

V

-

L

T

E

L

A

S

C

D

D

Q

D

V

A

V

L

V

A

F

L

K

K

R

R

L

L

V

V

R

R

Q

L

V

M

A

A

L

E

K

K

H

H

G

K

V

M

Y

H

A

A

T

T

F

F

M

M

A

A

K

K

P

P

M

Y

A

E

D

E

Q

H

P

A

G

G

S

S

A

G

M

M

H
|
H

I

I

H

H

Q

I

S

S

L

M

V

-

E

Q

I

N

G

N

T

R

G

G

K

E

N

N

L

V

F

L

V

S

R

D

D

A

D

E

G

G

S

E

D

D

S

S

P

P

M

L

F

L

R

K

H

K

Y

M

I

L

G

A

G

G

L

M

Q

I

A

D

L

L

L

M

A

P

Q

S

V

S

T

M

P

A

L

L

F

L

A

A

P

P

N

N

I

V

N

N

S

S

F

Y

R

R

M

F

R

Q

P

P

H

G

H

M

S

Y

A

V

P

P

I

T

N

Q

L

A

E

S

C

W

G

G

Y

H

D

N

N

N

R

R

S

T

C

V

G

A

L

L

R
|
R

V

I

P

P

L

C

S

G

D

D

P

R

A

H

N

N

R

H

R

R

L

V

E

E

N

Y

R

R

L

V

P

A

G

G

A

A

D

D

A

A

N

N

P

P

Y

Y

L

L

A

V

M

M

A

A

S

I

L

F

A

A

C

G

G

I

Y

L

I

H

G

G

I

L

M

D

E

N

R

E

I

L

E

P

P

L

S

Q

I

E

M

E

V

V

E

E

G

G

G

N

N

G

A

L

Y

E

R

Q

R

E

S

G

R

L

T

P

L

F

P

P

R

I

T

R

L

Q

D

S

E

D

A

A

L

L

D

G

R

E

L

F

E

I

K

E

C

N

T

D

V

H

I

L

K

R

N

R

V

Y

L

L

G

G

E

E

H

R

F

F

F

C

E

H

A

V

F

Y

T

H

M

A

I

C

K

K

R

N

V

D

E

E

L

L

D

L

A

Q

F

F

Q

E

D

R

V

L

I

I

S

T

S

E

W

T

E

E

R

I

N

E

H

W

L

M

L

L

H282 (= H259) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R335) mutation to Q: Activity is impaired to 3% of wild-type.

8ufjB Glutamine synthetase (see paper)
30% identity, 98% coverage: 9:447/450 of query aligns to 14:441/444 of 8ufjB

query
sites
8ufjB

I

V

N

K

E

E

H

R

G

D

I

V

A

K

E

F

V

I

E

R

C

T

L

Q

L

F

S

T

D

D

M

T

N

L

G

G

I

I

V

I

R

K

G

S

K

W

V

A

L

I

P

P

A

A

R

E

K

Q

F

L

L

E

R

E

S

A

L

F

E

E

D

N

N

G

S

V

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

Q

G

G

E

F

Y

T

P

R

F

I

A

E

D

E

D

S

N

D

N

M

T

K

V

L

V

A

I

L

D

D

P

P

D

S

A

T

D

-

L

-

R

-

P

-

D

-

F

F

R

R

T

I

L

L

C

P

V

W

A

R

P

P

G

A

Y

-

R

T

T

G

P

A

T

V

A

A

F

R

V

I

V

L

V

G

D

D

A

V

Y

Y

K

L

S

P

N

D

G

G

I

N

P

P

L

F

D

K

L

G

S

D

P

P

R

R

Q

Y

V

V

L

L

K

K

R

T

V

A

L

I

A

K

L

E

Y

A

S

E

E

K

Q

M

G

G

W

F

K

S

P

M

V

N

V

V

A

G

P

P

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

A

A

D

N

P

G

D

N

L

-

P

P

L

T

S

T

P

E

P

L

A

T

G

D

R

Q

S

G

G

G

R

Y

P

F

E

D

T

F

S

A

P

P

Q

L

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

Q

D

D

L

V

I

R

E

R

N

D

I

I

Y

D

E

Y

H

A

A

L

E

E

I

H

A

M

G

G

L

F

Q

Q

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

S

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

R

H

F

G

G

D

D

P

V

I

L

T

C

L

T

S

A

D

D

Q

N

V

V

V

T

F

F

K

K

R

Y

L

V

V

V

R

K

Q

S

V

I

A

A

L

Y

K

H

H

K

G

G

V

Y

Y

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

S

H

N

Q

Q

S

S

L

L

V

F

E

K

I

-

G

-

T

D

G

G

K

K

N

N

L

V

F

F

V

Y

R

D

D

P

D

D

G

T

-

P

-

T

-

K

-

L

S

S

D

Q

S

D

P

A

M

M

F

Y

R

-

H

-

Y

Y

I

I

G

G

L

L

Q

L

A

K

L

H

L

I

A

R

Q

E

V

F

T

T

P

A

L

V

F

T

A

N

P

P

N

V

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H

G

H

Y

S

E

A

A

P

P

I

V

N

Y

L

I

E

S

C

W

G

S

Y

A

D

Q

N

N

R

R

S

S

C

S

G

L

L

I

R

R

V

I

P

P

L

A

S

T

D

R

P

G

A

N

N

G

R

T

R

R

L

I

E
|
E

N

L

R

R

L

C

P

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

A

L

S

M

L

L

A

R

C

A

G

G

Y

L

I

E

G

G

I

I

M

K

E

N

R

K

I

I

E

D

P

P

S

G

-

E

-

P

-

T

-

N

-

V

-

N

-

I

-

F

I

H

M

L

V

S

E

D

G

K

G

E

N

R

A

E

Y

E

R

R

R

G

S

I

R

R

T

S

L

L

P

P

R

A

T

D

L

L

D

K

E

E

A

A

L

I

D

D

R

E

L

M

E

K

K

G

C

S

T

K

V

F

I

V

K

K

N

E

V

A

L

L

G

G

E

E

H

H

F

V

F

F

E

S

A

H

F

Y

T

L

M

C

I

A

K

K

R

E

V

M

E

E

L

W

D

D

A

E

F

Y

Q

K

D

A

V

V

I

V

S

H

S

P

W

W

E

E

R

L

N

S

H

R

L

Y

L

L

binding magnesium ion: E132 (= E138), E134 (= E140), E189 (= E203), E196 (= E210), H245 (= H259), E333 (= E347)

8tfkA Glutamine synthetase (see paper)
30% identity, 98% coverage: 9:447/450 of query aligns to 10:437/440 of 8tfkA

query
sites
8tfkA

I

V

N

K

E

E

H

R

G

D

I

V

A

K

E

F

V

I

E

R

C

T

L

Q

L

F

S

T

D

D

M

T

N

L

G

G

I

I

V

I

R

K

G

S

K

W

V

A

L

I

P

P

A

A

R

E

K

Q

F

L

L

E

R

E

S

A

L

F

E

E

D

N

N

G

S

V

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

Q

G

G

E

F

Y

T

P

R

F

I

A

E

D

E

D

S

N

D

N

M

T

K

V

L

V

A

I

L

D

D

P

P

D

S

A

T

D

-

L

-

R

-

P

-

D

-

F

F

R

R

T

I

L

L

C

P

V

W

A

R

P

P

G

A

Y

-

R

T

T

G

P

A

T

V

A

A

F

R

V

I

V

L

V

G

D

D

A

V

Y

Y

K

L

S

P

N

D

G

G

I

N

P

P

L

F

D

K

L

G

S

D

P

P

R

R

Q

Y

V

V

L

L

K

K

R

T

V

A

L

I

A

K

L

E

Y

A

S

E

E

K

Q

M

G

G

W

F

K

S

P

M

V

N

V

V

A

G

P

P

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

A

A

D

N

P

G

D

N

L

-

P

P

L

T

S

T

P

E

P

L

A

T

G

D

R

Q

S

G

G

G

R

Y

P

F

E

D

T

F

S

A

P

P

Q

-

P

-

Y

-

G

-

L

-

E

-

A

-

I

L

T

D

E

R

F

A

E

Q

D

D

L

V

I

R

E

R

N

D

I

I

Y

D

E

Y

H

A

A

L

E

E

I

H

A

M

G

G

L

F

Q

Q

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

S

Q

Q

L

H

E
|
E

I

I

N
x
D

F
|
F

L

R

H
x
F

G

G

D

D

P

V

I

L

T

C

L

T

S

A

D

D

Q

N

V

V

V

T

F

F

K

K

R

Y

L

V

V

V

R

K

Q

S

V

I

A

A

L

Y

K

H

H

K

G

G

V

Y

Y

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

S

H
x
N

Q

Q

S

S

L

L

V

F

E

K

I

-

G

-

T

D

G

G

K

K

N

N

L

V

F

F

V

Y

R

D

D

P

D

D

G

T

-

P

-

T

-

K

-

L

S

S

D

Q

S

D

P

A

M

M

F

Y

R

-

H

-

Y

Y

I

I

G

G

L

L

Q

L

A

K

L

H

L

I

A

R

Q

E

V

F

T

T

P

A

L

V

F

T

A

N

P

P

N

V

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

V

N

Y

L

I

E

S

C

W

G

S

Y

A

D

Q

N

N

R
|
R

S

S

C

S

G

L

L

I

R
|
R

V

I

P

P

L

A

S

T

D

R

P

G

A

N

N
x
G

R

T

R
|
R

L

I

E
|
E

N

L

R
|
R

L

C

P

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

A

L

S

M

L

L

A

R

C

A

G

G

Y

L

I

E

G

G

I

I

M

K

E

N

R

K

I

I

E

D

P

P

S

G

-

E

-

P

-

T

-

N

-

V

-

N

-

I

-

F

I

H

M

L

V

S

E

D

G

K

G

E

N

R

A

E

Y

E

R

R

R

G

S

I

R

R

T

S

L

L

P

P

R

A

T

D

L

L

D

K

E

E

A

A

L

I

D

D

R

E

L

M

E

K

K

G

C

S

T

K

V

F

I

V

K

K

N

E

V

A

L

L

G

G

E

E

H

H

F

V

F

F

E

S

A

H

F

Y

T

L

M

C

I

A

K

K

R

E

V

M

E

E

L

W

D

D

A

E

F

Y

Q

K

D

A

V

V

I

V

S

H

S

P

W

W

E

E

R

L

N

S

H

R

L

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E138), D194 (≠ N212), F195 (= F213), F197 (≠ H215), N243 (≠ H261), R312 (= R330), R317 (= R335), G325 (≠ N343), R327 (= R345)
binding magnesium ion: E128 (= E138), E128 (= E138), E130 (= E140), E185 (= E203), E192 (= E210), E192 (= E210), H241 (= H259), E329 (= E347)
binding l-methionine-s-sulfoximine phosphate: E128 (= E138), E130 (= E140), E185 (= E203), E192 (= E210), G237 (= G255), H241 (= H259), R294 (= R312), E300 (≠ S318), R312 (= R330), R331 (= R349)

7tenA Glutamine synthetase (see paper)
32% identity, 98% coverage: 8:447/450 of query aligns to 10:439/442 of 7tenA

query
sites
7tenA

W

F

I

A

N

D

E

E

H

Q

G

N

I

V

A

K

E

F

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

I

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

K

R

K

S

A

L

L

E

D

D

N

N

K

S

I

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N

F

T

V

V

R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

F

P

P

D

D

F

L

R

D

T

T

L

W

C

V

V

V

A

F

P

P

-

W

-

T

G

A

Y

E

R

K

T

G

P

K

T

V

A

A

F

R

V

M

V

I

V

C

D

D

A

I

Y

Y

K

N

S

P

N

D

G

M

I

T

P

P

L

F

D

A

L

G

S

D

P

P

R

R

Q

A

V

N

L

L

K

K

R

R

V

V

L

L

A

K

L

E

Y

M

S

E

E

E

Q

L

G

G

W

F

K

T

P

E

V

F

-

N

V

L

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

-

D

-

E

N

N

A

R

D

R

P

P

D

T

L

L

P

E

L

L

S

N

P

D

P

S

A

G

G

G

R

Y

S

F

G

-

R

-

P

-

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F
|
F

L
x
K

H
x
Y

G

E

D

D

P

A

I

I

T

T

L

A

S

C

D

D

Q

S

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H
x
N

Q

M

S
|
S

L

L

V

-

E

-

I

F

G

N

T

E

G

K

K

G

N

N

L

A

F

F

V

F

R

D

D

E

D

S

G

G

-

E

-

L

S

E

D

L

S

S

P

Q

M

T

F

A

R

Y

H

H

Y

F

I

L

G

A

G

G

L

M

Q

L

A

K

L

H

L

A

A

R

Q

G

V

Y

T

T

P

A

L

V

F

T

A

N

P

P

N

T

I

I

N

N

S

S

F

F

R

K

R
|
R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

C

N

Y

L

I

E

A

C

W

G

S

Y

G

D

K

N

N

R
|
R

S

S

C

P

G

L

L

V

R
|
R

V

V

P

P

L

S

S

S

D

R

P

G

A

L

N
x
S

R

T

R
|
R

L

L

E

E

N

L

R
|
R

L

S

P

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

M

M

A

A

A

V

S

L

L

L

A

K

C

A

G

G

Y

L

I

S

G

G

I

I

M

K

E

D

R

E

I

L

E

T

P

P

S

P

I

A

M

P

V

V

E

D

-

R

-

N

-

I

-

Y

G

G

G

M

N

N

A

E

Y

E

R

E

R

R

S

E

R

A

T

T

-

G

-

I

-

Y

-

D

L

L

P

P

R

E

T

S

L

L

D

G

E

H

A

A

L

L

D

I

R

E

L

L

E

E

K

K

C

N

T

E

V

I

I

I

K

K

N

D

V

G

L

L

G

G

E

E

H

H

F

I

F

F

E

E

A

H

F

F

T

I

M

E

I

A

K

K

R

T

V

I

E

E

L

C

D

D

A

M

F

F

Q

R

D

T

V

A

I

V

S

H

S

P

W

W

E

E

R

R

N

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A136), E130 (= E138), E182 (≠ D198), D196 (≠ N212), F197 (= F213), K198 (≠ L214), Y199 (≠ H215), N245 (≠ H261), S247 (= S263), R319 (= R335), S327 (≠ N343), R329 (= R345)
binding l-methionine-s-sulfoximine phosphate: E130 (= E138), E132 (= E140), E187 (= E203), E194 (= E210), N238 (≠ P254), G239 (= G255), H243 (= H259), R296 (= R312), E302 (≠ S318), R314 (= R330), R333 (= R349)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
32% identity, 98% coverage: 8:447/450 of query aligns to 11:440/443 of 7tf9S

query
sites
7tf9S

W

F

I

A

N

D

E

E

H

Q

G

N

I

V

A

K

E

F

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

I

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

K

R

K

S

A

L

L

E

D

D

N

N

K

S

I

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N
x
F

T
x
V

V

R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

F

P

P

D

D

F

L

R

D

T

T

L

W

C

V

V

V

A

F

P

P

-

W

-

T

G

A

Y

E

R

K

T

G

P

K

T

V

A

A

F

R

V

M

V

I

V

C

D

D

A

I

Y

Y

K

N

S

P

N

D

G

M

I

T

P

P

L

F

D

A

L

G

S

D

P

P

R

R

Q

A

V

N

L

L

K

K

R

R

V

V

L

L

A

K

L

E

Y

M

S

E

E

E

Q

L

G

G

W

F

K

T

P

E

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

-

D

-

E

N

N

A

R

D

R

P

P

D

T

L

L

P

E

L

L

S

N

P

D

P

S

A

G

G

G

R
x
Y

S

F

G

-

R

-

P

-

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

E

D

D

P

A

I

I

T

T

L

A

S

C

D

D

Q

S

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

F

H

N

Q

M

S

S

L

L

V

-

E

-

I

F

G

N

T

E

G

K

K

G

N

N

L

A

F

F

V

F

R

D

D

E

D

S

G

G

-

E

-

L

S

E

D

L

S

S

P

Q

M

T

F

A

R

Y

H

H

Y

F

I

L

G

A

G

G

L

M

Q

L

A

K

L

H

L

A

A

R

Q

G

V

Y

T

T

P

A

L

V

F

T

A

N

P

P

N

T

I

I

N

N

S

S

F

F

R

K

R
|
R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

C

N

Y

L

I

E

A

C

W

G

S

Y

G

D

K

N

N

R

R

S

S

C

P

G

L

L

V

R

R

V

V

P

P

L

S

S

S

D

R

P

G

A

L

N

S

R

T

R

R

L

L

E
|
E

N

L

R

R

L

S

P

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

M

M

A

A

A

V

S

L

L

L

A

K

C

A

G

G

Y

L

I

S

G

G

I

I

M

K

E

D

R

E

I

L

E

T

P

P

S

P

I

A

M

P

V

V

E

D

-

R

-

N

-

I

-

Y

G

G

G

M

N

N

A

E

Y

E

R

E

R

R

S

E

R

A

T

T

-

G

-

I

-

Y

-

D

L

L

P

P

R

E

T

S

L

L

D

G

E

H

A

A

L

L

D

I

R

E

L

L

E

E

K

K

C

N

T

E

V

I

I

I

K

K

N

D

V

G

L

L

G

G

E

E

H

H

F

I

F

F

E

E

A

H

F

F

T

I

M
x
E

I

A

K

K

R

T

V
x
I

E

E

L

C

D

D

A
x
M

F

F

Q

R

D

T

V

A

I

V

S

H

S

P

W

W

E

E

R

R

N

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E140), Y155 (≠ R160), E188 (= E203), G240 (= G255), G242 (≠ A257), R297 (= R312), E303 (≠ S318)
binding magnesium ion: E131 (= E138), E133 (= E140), E188 (= E203), E195 (= E210), H244 (= H259), E332 (= E347)
binding : F59 (≠ N69), V60 (≠ T70), E418 (≠ M425), I422 (≠ V429), M426 (≠ A433)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
32% identity, 83% coverage: 74:447/450 of query aligns to 69:445/447 of 8oooA

query
sites
8oooA

D

E

P

S

D

D

A

M

D

M

L

L

R

K

P

P

D

D

F

L

R

S

T

T

L

F

C

S

V

V

A

L

P

P

G

W

-
x
R

-

P

Y

S

R

E

T

K

P

S

T
x
V

A

A

F

R

V

V

V

I

V

C

D

D

A

V

Y

Y

K

T

S

T

N

K

G

G

I

K

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

G

V

C

L

L

K

K

R

R

V

V

L

M

A

E

L

E

Y

F

S

K

E

K

Q

E

-

F

G

N

W

G

K

E

P

Y

V

F

V

V

A

G

P

P

E
|
E

I

P

E

E

F

F

Y

F

L

L

T

-

Q

-

V

L

N

K

A

K

D

D

P

P

D

H

L

N

P

P

L

H

S

K

P

Y

-

I

P

P

A

A

G

D

R

D

S

G

G

G

R

Y

P

-

E

-

T

-

S

-

P

-

Q

-

P

-

Y

F

G

D

L

L

E

E

A

P

I

M

T

D

E

E

F

A

E
x
P

D

D

L

I

I
x
R

E
x
R

N

D

I

I

Y

V

E

F

H

A

A

L

E

E

I

N

A

L

G

G

L

F

Q

H

I

V

D
x
E

T

A

L
x
S

I

H

H

H

E

E

A

V

G

A

A

P

A

G

Q

Q

L

H

E

E

I

V

N

D

F
|
F

L
x
K

H
x
F

G

D

D

D

P

A

I

L

T

K

L

T

S

A

D

D

Q

S

V

V

V

I

V

T

F

F

K

K

R

T

L

T

V

I

R

K

Q

T

V

I

A

A

L

E

K

Q

H

H

G

G

V

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

F

A

F

D

G

Q

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H
|
H

Q

Q

S
|
S

L

I

V

-

E

-

I

W

G

L

T

N

G

G

K

E

N

P

L

S

F

F

V

Y

R

D

D

E

D

N

G

A

-

P

-

Y

-

Q

-

L

S

S

D

E

S

T

P

C

M

M

F

-

R

-

H

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

A

K

L

H

L

A

A

K

Q

A

V

I

T

V

P

A

L

I

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H

G

H

Y

S

E

A

A

P

P

I

V

N

N

L

I

E

A

C

W

G

A

Y

N

D

S

N

N

R

R

S

S

C

A

G

I

L

I

R
|
R

V

V

P

P

L

A

S

A

D

R

P

G

A

K

N

G

R

T

R
|
R

L

I

E

E

N

F

R

R

L

A

P

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

T

A

V

S

M

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

V

M

K

E

N

R

K

I

L

-

D

-

A

-

P

E

E

P

P

S

V

-

E

-

R

-

N

-

I

-

F

I

A

M

M

V

S

E

E

G

A

G

E

N

K

A

K

Y

E

R

L

R

G

S

I

R

E

T

S

L

V

P

P

R

A

T

N

L

L

D

K

E

A

A

A

L

L

D

D

R

E

L

L

E

E

K

N

C

N

T

D

V

V

I

L

K

K

N

N

V

A

L

L

G

G

E

K

H

H

F

I

F

F

E

E

A

S

F

F

T

L

M

E

I

I

K

K

R

N

V

A

E

E

L

W

D

D

A

S

F

F

Q

R

D

T

V

S

I

V

S

T

S

D

W

W

E

E

R

T

N

T

H

A

L

Y

L

L

binding 2-oxoglutaric acid: R87 (vs. gap), V93 (≠ T96), P170 (≠ E180), R173 (≠ I183), R174 (≠ E184), S190 (≠ L200)
binding adenosine-5'-triphosphate: E136 (= E138), E188 (≠ D198), F203 (= F213), K204 (≠ L214), F205 (≠ H215), H251 (= H261), S253 (= S263), R325 (= R335), R335 (= R345)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
32% identity, 83% coverage: 74:447/450 of query aligns to 68:444/446 of 8ooqB

query
sites
8ooqB

D

E

P

S

D

D

A

M

D

M

L

L

R

K

P

P

D

D

F

L

R

S

T

T

L

F

C

S

V

V

A

L

P

P

G

W

-
x
R

-

P

Y

S

R

E

T

K

P

S

T
x
V

A

A

F

R

V

V

V

I

V

C

D

D

A

V

Y

Y

K

T

S

T

N

K

G

G

I

K

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

G

V

C

L

L

K

K

R

R

V

V

L

M

A

E

L

E

Y

F

S

K

E

K

Q

E

-

F

G

N

W

G

K

E

P

Y

V

F

V

V

A

G

P

P

E

E

I

P

E
|
E

F

F

Y

F

L

L

T

-

Q

-

V

L

N

K

A

K

D

D

P

P

D

H

L

N

P

P

L

H

S

K

P

Y

-

I

P

P

A

A

G

D

R

D

S

G

G

G

R

Y

P

-

E

-

T

-

S

-

P

-

Q

-

P

-

Y

F

G

D

L

L

E

E

A

P

I

M

T

D

E

E

F

A

E
x
P

D

D

L

I

I
x
R

E
x
R

N

D

I

I

Y

V

E

F

H

A

A

L

E

E

I

N

A

L

G

G

L

F

Q

H

I

V

D

E

T

A

L
x
S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

V

N

D

F

F

L

K

H

F

G

D

D

D

P

A

I

L

T

K

L

T

S

A

D

D

Q

S

V

V

V

I

V

T

F

F

K

K

R

T

L

T

V

I

R

K

Q

T

V

I

A

A

L

E

K

Q

H

H

G

G

V

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

F

A

F

D

G

Q

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H

H

Q

Q

S

S

L

I

V

-

E

-

I

W

G

L

T

N

G

G

K

E

N

P

L

S

F

F

V

Y

R

D

D

E

D

N

G

A

-

P

-

Y

-

Q

-

L

S

S

D

E

S

T

P

C

M

M

F

-

R

-

H

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

A

K

L

H

L

A

A

K

Q

A

V

I

T

V

P

A

L

I

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H

G

H

Y

S

E

A

A

P

P

I

V

N

N

L

I

E

A

C

W

G

A

Y

N

D

S

N

N

R

R

S

S

C

A

G

I

L

I

R

R

V

V

P

P

L

A

S

A

D

R

P

G

A

K

N

G

R

T

R

R

L

I

E

E

N

F

R

R

L

A

P

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

T

A

V

S

M

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

V

M

K

E

N

R

K

I

L

-

D

-

A

-

P

E

E

P

P

S

V

-

E

-

R

-

N

-

I

-

F

I

A

M

M

V

S

E

E

G

A

G

E

N

K

A

K

Y

E

R

L

R

G

S

I

R

E

T

S

L

V

P

P

R

A

T

N

L

L

D

K

E

A

A

A

L

L

D

D

R

E

L

L

E

E

K

N

C

N

T

D

V

V

I

L

K

K

N

N

V

A

L

L

G

G

E

K

H

H

F

I

F

F

E

E

A

S

F

F

T

L

M

E

I

I

K

K

R

N

V

A

E

E

L

W

D

D

A

S

F

F

Q

R

D

T

V

S

I

V

S

T

S

D

W

W

E

E

R

T

N

T

H

A

L

Y

L

L

binding 2-oxoglutaric acid: R86 (vs. gap), V92 (≠ T96), P169 (≠ E180), R172 (≠ I183), R173 (≠ E184), S189 (≠ L200)
binding magnesium ion: E137 (= E140), E192 (= E203), E199 (= E210)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
29% identity, 97% coverage: 11:447/450 of query aligns to 13:436/439 of 7tdpA

query
sites
7tdpA

E

E

H

E

G

N

I

V

A

R

E

F

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

L

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

E

R

K

S

A

L

L

E

D

D

N

N

K

S

M

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N

Y

T

V

V

R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

Y

P

P

D

D

F

L

R

D

T

T

L

W

C

V

V

V

A

F

P

P

G

-

Y

W

R

S

T

D

P

R

T

V

A

A

F

R

V

L

V

I

V

C

D

D

A

I

Y

Y

K

K

S

P

N

D

G

G

I

S

P

P

L

F

D

A

L

G

S

D

P

P

R

R

Q

G

V

I

L

L

K

K

R

R

V

V

L

L

A

K

L

E

Y

A

S

E

E

E

Q

L

G

G

W

Y

K

T

P

S

V

M

-
x
N

V

V

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

T

N

D

A

E

D

K

P

G

D

D

L

-

P

P

L

T

S

T

P

E

P

L

A

N

G

D

R

Q

S

G

G

G

R

Y

P

F

E

D

T

L

S

A

P

P

Q

M

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

E

I

I

Y

V

E

L

H

K

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F

F

L

K

H
x
Y

G

A

D

D

P

A

I

V

T

K

L

A

S

A

D

D

Q

Q

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

Q

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

Q

S
|
S

L

L

V

F

E

K

I

-

G

-

T

D

G

N

K

E

N

N

L

V

F

F

V

Y

-

D

-

E

R

T

D

D

D

E

G

L

S

G

D

L

S

S

P

Q

M

T

F

A

R

R

H

H

Y

Y

I

M

G

A

G

G

L

I

Q

L

A

K

L

H

L

A

A

R

Q

A

V

M

T

A

P

A

L

I

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

C

W

G

S

Y

A

D

S

N

N

R
|
R

S

S

C

P

G

M

L

I

R
|
R

V

I

P

P

L

A

S

S

D

R

P

G

A

L

N

S

R

T

R
|
R

L

V

E
|
E

N

V

R
|
R

L

N

P

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

A

A

V

S

M

L

L

A

R

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

R

R

Q

I

M

E

A

-

L

P

P

S

A

-

P

-

I

-

D

-

R

-

N

-

I

-

Y

I

V

M

M

V

S

E

E

G

E

N

R

A

I

Y

E

R

E

R

G

S

I

R

P

T

S

L

L

P

P

R

A

T

D

L

L

D

K

E

E

A

A

L

L

D

S

R

E

L

L

E

I

K

R

C

S

T

E

V

V

I

I

K

S

N

D

V

A

L

L

G

G

E

D

H

H

F

A

F

L

E

A

A

Y

F

F

T

Y

M

E

I

L

K

K

R

E

V

I

E

E

L

W

D

D

A

M

F

Y

Q

R

D

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

N

D

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A136), E127 (= E138), E179 (≠ D198), D193 (≠ N212), Y196 (≠ H215), N242 (≠ H261), S244 (= S263), R316 (= R335), R326 (= R345)
binding magnesium ion: E127 (= E138), E127 (= E138), E129 (= E140), E184 (= E203), E191 (= E210), E191 (= E210), H240 (= H259), E328 (= E347)
binding l-methionine-s-sulfoximine phosphate: E127 (= E138), E129 (= E140), E184 (= E203), E191 (= E210), G236 (= G255), H240 (= H259), R293 (= R312), E299 (≠ S318), R311 (= R330), R330 (= R349)

7tfaB Glutamine synthetase (see paper)
29% identity, 97% coverage: 11:447/450 of query aligns to 13:438/441 of 7tfaB

query
sites
7tfaB

E

E

H

E

G

N

I

V

A

R

E

F

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

L

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

E

R

K

S

A

L

L

E

D

D

N

N

K

S

M

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N
x
Y

T

V

V
x
R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

Y

P

P

D

D

F

L

R

D

T

T

L

W

C

V

V

V

A

F

P

P

G

W

Y

V

R

T

T

S

P

D

-

R

T

V

A

A

F

R

V

L

V

I

V

C

D

D

A

I

Y

Y

K

K

S

P

N

D

G

G

I

S

P

P

L

F

D

A

L

G

S

D

P

P

R

R

Q

G

V

I

L

L

K

K

R

R

V

V

L

L

A

K

L

E

Y

A

S

E

E

E

Q

L

G

G

W

Y

K

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

T

N

D

A

E

D

K

P

G

D

D

L

-

P

P

L

T

S

T

P

E

P

L

A

N

G

D

R

Q

S

G

G

G

R
x
Y

P

F

E

D

T

L

S

A

P

P

Q

M

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

E

I

I

Y

V

E

L

H

K

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A
x
V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

D

P

A

I

V

T

K

L

A

S

A

D

D

Q

Q

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

Q

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

Q

S

S

L

L

V

F

E

K

I

-

G

-

T

D

G

N

K

E

N

N

L

V

F

F

V

Y

-

D

-

E

R

T

D

D

D

E

G

L

S

G

D

L

S

S

P

Q

M

T

F

A

R

R

H

H

Y

Y

I

M

G

A

G

G

L

I

Q

L

A

K

L

H

L

A

A

R

Q

A

V

M

T

A

P

A

L

I

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H
x
G

H
x
Y

S
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

C

W

G

S

Y

A

D

S

N

N

R
|
R

S

S

C

P

G

M

L

I

R

R

V

I

P

P

L

A

S

S

D

R

P

G

A

L

N

S

R

T

R

R

L

V

E
|
E

N

V

R

R

L

N

P

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

A

A

V

S

M

L

L

A

R

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

R

R

Q

I

M

E

A

-

L

P

P

S

A

-

P

-

I

-

D

-

R

-

N

-

I

-

Y

I

V

M

M

V

S

E

E

G

E

N

R

A

I

Y

E

R

E

R

G

S

I

R

P

T

S

L

L

P

P

R

A

T

D

L

L

D

K

E

E

A

A

L

L

D

S

R

E

L

L

E

I

K

R

C

S

T

E

V

V

I

I

K

S

N

D

V

A

L

L

G

G

E

D

H

H

F

A

F

L

E

A

A

Y

F

F

T

Y

M

E

I

L

K

K

R

E

V

I

E

E

L

W

D

D

A
x
M

F

Y

Q

R

D

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

N

D

H

Q

L

Y

L

L

binding glutamine: E131 (= E140), Y153 (≠ R163), E186 (= E203), G238 (= G255), H242 (= H259), R295 (= R312), E301 (≠ S318)
binding magnesium ion: E129 (= E138), E131 (= E140), E186 (= E203), E193 (= E210), H242 (= H259), E330 (= E347)
binding : Y58 (≠ N69), R60 (≠ V71), V187 (≠ A204), N237 (≠ P254), G299 (≠ H316), Y300 (≠ H317), R313 (= R330), M424 (≠ A433)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
28% identity, 99% coverage: 4:447/450 of query aligns to 7:440/443 of 4lnkA

query
sites
4lnkA

E

D

F

I

E

E

A

K

W

L

I

V

N

K

E

E

H

E

G

N

I

V

A

K

E

Y

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

G

R
x
K

S

A

L

L

E

D

D

N

N

K

S

V

L
x
M

R

F

I
x
D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N

F

T

V

V

R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

Y

P

P

D

D

F

L

R

N

T

T

L

F

C

V

V

I

A

F

P

P

-

W

-

T

G

A

Y

E

R

K

T

G

P

K

T

V

A

A

F

R

V

F

V

I

V

C

D

D

A

I

Y

Y

K

N

S

P

N

D

G

G

I

T

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

N

V

N

L

L

K

K

R

R

V

I

L

L

A

K

L

E

Y

M

S

E

E

D

Q

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

-

E

-

K

A

G

D

E

P

P

D

T

L

L

P

E

L

L

S

N

P

-

A

-

G

D

R

K

S

G

G

G

R

Y

P

F

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A
x
V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F
|
F

L

K

H
x
Y

G

A

D

G

P

A

I

V

T

R

L

S

S

C

D

D

Q

D

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

L

L

V

F

E

K

I

-

G

-

T

N

G

G

K

V

N

N

L

A

F

F

V

F

R

-

D

D

D

E

G

N

S

A

D

D

-

L

-

Q

-

L

S

S

P

E

M

T

F

A

R

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

A

K

L

H

L

A

A

T

Q

S

V

F

T

T

P

A

L

V

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

C

W

G

S

Y

A

D

Q

N

N

R
|
R

S

S

C

P

G

L

L

I

R

R

V

I

P

P

L

A

S
|
S

D

R

P

G

A

I

N
x
S

R

T

R
|
R

L

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

L

E

E

P

A

S

P

I

A

M

P

V

I

E

D

G

-

G

R

N

N

A

I

Y

Y

R

V

R

M

S

S

R

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

T

T

L

L

D

A

E

E

A

A

L

L

D

E

R

E

L

F

E

K

K

S

C

N

T

E

V

V

I

M

K

V

N

K

V

A

L

L

G

G

E

E

H

H

F

L

F

F

E

E

A

H

F

F

T

I

M

E

I

A

K

K

R

E

V

I

E

E

L

W

D

D

A

M

F

F

Q

R

D

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

N

E

H

Q

L

Y

L

M

active site: D52 (≠ I49), E131 (= E138), E133 (= E140), E188 (= E203), E195 (= E210), H244 (= H259), R315 (= R330), E332 (= E347), R334 (= R349)
binding adenosine-5'-diphosphate: K43 (≠ R40), M50 (≠ L47), F198 (= F213), Y200 (≠ H215), N246 (≠ H261), S248 (= S263), S324 (= S339), S328 (≠ N343), R330 (= R345)
binding glutamic acid: E133 (= E140), E188 (= E203), V189 (≠ A204), N239 (≠ P254), G240 (= G255), G242 (≠ A257), E303 (≠ S318)
binding magnesium ion: E131 (= E138), E188 (= E203), E195 (= E210), H244 (= H259), E332 (= E347)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
28% identity, 99% coverage: 4:447/450 of query aligns to 7:440/443 of 4lniA

query
sites
4lniA

E

D

F

I

E

E

A

K

W

L

I

V

N

K

E

E

H

E

G

N

I

V

A

K

E

Y

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

G

R

K

S

A

L

L

E

D

D

N

N

K

S

V

L

M

R

F

I
x
D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N

F

T

V

V

R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

Y

P

P

D

D

F

L

R

N

T

T

L

F

C

V

V

I

A

F

P

P

-

W

-

T

G

A

Y

E

R

K

T

G

P

K

T

V

A

A

F

R

V

F

V

I

V

C

D

D

A

I

Y

Y

K

N

S

P

N

D

G

G

I

T

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

N

V

N

L

L

K

K

R

R

V

I

L

L

A

K

L

E

Y

M

S

E

E

D

Q

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

-

E

-

K

A

G

D

E

P

P

D

T

L

L

P

E

L

L

S

N

P

-

A

-

G

D

R

K

S

G

G

G

R

Y

P

F

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F

F

L

K

H
x
Y

G

A

D

G

P

A

I

V

T

R

L

S

S

C

D

D

Q

D

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

L

L

V

F

E

K

I

-

G

-

T

N

G

G

K

V

N

N

L

A

F

F

V

F

R

-

D

D

D

E

G

N

S

A

D

D

-

L

-

Q

-

L

S

S

P

E

M

T

F

A

R

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

A

K

L

H

L

A

A

T

Q

S

V

F

T

T

P

A

L

V

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

C

W

G

S

Y

A

D

Q

N

N

R
|
R

S

S

C

P

G

L

L

I

R
|
R

V

I

P

P

L

A

S

S

D

R

P

G

A

I

N

S

R

T

R
|
R

L

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

L

E

E

P

A

S

P

I

A

M

P

V

I

E

D

G

-

G

R

N

N

A

I

Y

Y

R

V

R

M

S

S

R

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

T

T

L

L

D

A

E

E

A

A

L

L

D

E

R

E

L

F

E

K

K

S

C

N

T

E

V

V

I

M

K

V

N

K

V

A

L

L

G

G

E

E

H

H

F

L

F

F

E

E

A

H

F

F

T

I

M

E

I

A

K

K

R

E

V

I

E

E

L

W

D

D

A

M

F

F

Q

R

D

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

N

E

H

Q

L

Y

L

M

active site: D52 (≠ I49), E131 (= E138), E133 (= E140), E188 (= E203), E195 (= E210), H244 (= H259), R315 (= R330), E332 (= E347), R334 (= R349)
binding adenosine-5'-diphosphate: E131 (= E138), E183 (≠ D198), D197 (≠ N212), Y200 (≠ H215), N246 (≠ H261), S248 (= S263), R320 (= R335), R330 (= R345)
binding magnesium ion: E131 (= E138), E131 (= E138), E133 (= E140), E188 (= E203), E195 (= E210), E195 (= E210), H244 (= H259), E332 (= E347)
binding l-methionine-s-sulfoximine phosphate: E133 (= E140), E188 (= E203), H244 (= H259), R297 (= R312), E303 (≠ S318), R315 (= R330), R334 (= R349)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
28% identity, 99% coverage: 4:447/450 of query aligns to 8:441/444 of P12425

query
sites
P12425

E

D

F

I

E

E

A

K

W

L

I

V

N

K

E

E

H

E

G

N

I

V

A

K

E

Y

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

G

R

K

S

A

L

L

E

D

D

N

N

K

S

V

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N
x
G

N

F

T

V

V
x
R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

Y

P

P

D

D

F

L

R

N

T

T

L

F

C

V

V

I

A

F

P

P

-

W

-

T

G

A

Y

E

R

K

T

G

P

K

T

V

A

A

F

R

V

F

V

I

V

C

D

D

A

I

Y

Y

K

N

S

P

N

D

G

G

I

T

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

N

V

N

L

L

K

K

R

R

V

I

L

L

A

K

L

E

Y

M

S

E

E

D

Q

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

-

E

-

K

A

G

D

E

P

P

D

T

L

L

P

E

L

L

S

N

P

-

A

-

G

D

R

K

S

G

G

G

R

Y

P

F

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A
x
V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

G

P

A

I

V

T

R

L

S

S

C

D

D

Q

D

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

L

L

V

F

E

K

I

-

G

-

T

N

G

G

K

V

N

N

L

A

F

F

V

F

R

-

D

D

D

E

G

N

S

A

D

D

-

L

-

Q

-

L

S

S

P

E

M

T

F

A

R

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

A

K

L

H

L

A

A

T

Q

S

V

F

T

T

P

A

L

V

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H
x
G

H

Y

S
x
E

A

A

P
|
P

I

C

N

Y

L

V

E

A

C

W

G

S

Y

A

D

Q

N

N

R

R

S

S

C

P

G

L

L

I

R

R

V

I

P

P

L

A

S

S

D

R

P

G

A

I

N

S

R

T

R

R

L

V

E
|
E

N

V

R

R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

L

E

E

P

A

S

P

I

A

M

P

V

I

E

D

G

-

G

R

N

N

A

I

Y

Y

R

V

R

M

S

S

R

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

T

T

L

L

D

A

E

E

A

A

L

L

D

E

R

E

L

F

E

K

K

S

C

N

T

E

V

V

I

M

K

V

N

K

V

A

L

L

G

G

E

E

H

H

F

L

F

F

E

E

A

H

F

F

T

I

M

E

I

A

K

K

R

E

V

I

E
|
E

L

W

D

D

A

M

F

F

Q

R

D

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

N

E

H

Q

L

Y

L

M

G59 (≠ N68) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ V71) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E138) binding Mg(2+)
E134 (= E140) binding Mg(2+)
E189 (= E203) binding Mg(2+)
V190 (≠ A204) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E210) binding Mg(2+)
G241 (= G255) binding L-glutamate
H245 (= H259) binding Mg(2+)
G302 (≠ H316) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ S318) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P320) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E347) binding Mg(2+)
E424 (= E430) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
28% identity, 99% coverage: 4:447/450 of query aligns to 11:444/447 of 4s0rD

query
sites
4s0rD

E

D

F

I

E

E

A

K

W

L

I

V

N

K

E

E

H

E

G

N

I

V

A

K

E

Y

V

I

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

I

P

P

A

V

R

S

K

Q

F

L

L

G

R

K

S

A

L

L

E

D

D

N

N

K

S

V

L

M

R

F

I
x
D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N
x
F

T
x
V

V
x
R

V
x
I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

Y

P

P

D

D

F

L

R

N

T

T

L

F

C

V

V

I

A

F

P

P

-

W

-

T

G

A

Y

E

R

K

T

G

P

K

T

V

A

A

F

R

V

F

V

I

V

C

D

D

A

I

Y

Y

K

N

S

P

N

D

G

G

I

T

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

N

V

N

L

L

K

K

R

R

V

I

L

L

A

K

L

E

Y

M

S

E

E

D

Q

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

-

E

-

K

A

G

D

E

P

P

D

T

L

L

P

E

L

L

S

N

P

-

A

-

G

D

R

K

S

G

G

G

R

Y

P

F

E
x
D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

G

P

A

I

V

T

R

L

S

S

C

D

D

Q

D

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D
x
G

Q
x
V

P
x
N

G

G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

L

L

V

F

E

K

I

-

G

-

T

N

G

G

K

V

N

N

L

A

F

F

V

F

R

-

D

D

D

E

G

N

S

A

D

D

-

L

-

Q

-

L

S

S

P

E

M

T

F

A

R

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

A

K

L

H

L

A

A

T

Q

S

V

F

T

T

P

A

L

V

F

T

A

N

P

P

N

T

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H
x
G

H
x
Y

S
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

C

W

G

S

Y

A

D

Q

N

N

R
|
R

S

S

C

P

G

L

L

I

R

R

V

I

P

P

L

A

S

S

D

R

P

G

A

I

N

S

R

T

R

R

L

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

L

E

E

P

A

S

P

I

A

M

P

V

I

E

D

G

-

G

R

N

N

A

I

Y
|
Y

R

V

R

M

S

S

R

K

T

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

T

T

L

L

D

A

E

E

A

A

L

L

D

E

R

E

L

F

E

K

K

S

C

N

T

E

V

V

I

M

K

V

N

K

V

A

L

L

G

G

E

E

H

H

F

L

F

F

E

E

A
x
H

F

F

T

I

M

E

I

A

K

K

R

E

V
x
I

E

E

L

W

D

D

A
x
M

F

F

Q

R

D

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

N

E

H

Q

L

Y

L

M

active site: D56 (≠ I49), E135 (= E138), E137 (= E140), E192 (= E203), E199 (= E210), H248 (= H259), R319 (= R330), E336 (= E347), R338 (= R349)
binding glutamine: E137 (= E140), E192 (= E203), R301 (= R312), E307 (≠ S318)
binding magnesium ion: I66 (≠ V72), E135 (= E138), E135 (= E138), E199 (= E210), H248 (= H259), H248 (= H259), E336 (= E347), H419 (≠ A422)
binding : F63 (≠ N69), V64 (≠ T70), R65 (≠ V71), I66 (≠ V72), D161 (≠ E165), G241 (≠ D252), V242 (≠ Q253), N243 (≠ P254), G305 (≠ H316), Y306 (≠ H317), Y376 (= Y388), I426 (≠ V429), M430 (≠ A433)

8oozA Glutamine synthetase (see paper)
31% identity, 83% coverage: 76:447/450 of query aligns to 58:427/430 of 8oozA

query
sites
8oozA

D

D

A

M

D

V

L

L

R

R

P

P

D

D

F

P

R

D

T

T

L

F

C

R

V

V

A

L

P

P

G

-

Y

W

R

S

T

T

P

A

T

E

A

A

F

R

V

L

V

I

V

C

D

D

A

I

Y

E

K

L

S

P

N

D

G

G

I

K

P

P

L

F

D

M

L

G

S

C

P

P

R

R

Q

Q

V

V

L

L

K

K

R

K

V

N

L

M

A

E

L

E

Y

A

S

A

E

K

Q

L

G

G

W

Y

K

V

P

M

V

N

V

T

A
x
G

P

P

E
|
E

I

M

E

E

F

F

Y

F

L

L

T

F

Q

K

V

-

N

-

A

R

D

Q

P

D

D

G

L

M

P

P

L

T

S

N

P

I

P

P

A

Q

G

D

R

R

S

G

G

G

R

Y

P

F

E

D

T

L

S

A

P

P

Q

-

P

-

Y

-

G

-

L

-

E

-

A

-

I

I

T

D

E

L

F

A

E

E

D

E

L

I

I

K

E

R

N

E

I

I

Y

V

E

L

H

V

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

V

D
x
E

T

A

L

A

I

H

H

H

E

E

A

V

G

A

A

F

A

G

Q

Q

L

H

E

E

I

I

N

D

F
|
F

L
x
K

H
x
Y

G

D

D

N

P

A

I

L

T

A

L

T

S

A

D

D

Q

N

V

V

V

I

V

T

F

L

K

K

R

Y

L

V

V

A

R

K

Q

T

V

L

A

A

L

L

K

Q

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

I

A

F

D

G

Q

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

T

H
x
N

Q

T

S
|
S

L

L

V

F

E

K

I

-

G

-

T

D

G

G

K

K

N

N

L

A

F

F

V

Y

R

D

D

P

D

D

G

A

S

P

D

D

-

Q

-

I

S

S

P

D

M

T

F

L

R

R

H

Y

Y

F

I

V

G

G

L

V

Q

L

A

K

L

H

L

I

A

R

Q

A

V

I

T

T

P

A

L

I

F

T

A

N

P

P

N

L

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H

G

H

Y

S

E

A

A

P

P

I

V

N

Y

L

I

E

T

C

W

G

S

Y

G

D

P

N

N

R

R

S

S

C

S

G

L

L

I

R

R

V

V

P

P

L

A

S

P

D

R

P

G

A

N

N
x
S

R

T

R
|
R

L

I

E
|
E

N

I

R

R

L

S

P

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

S

I

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

V

M

K

E

N

R

K

I

I

E

E

P

P

S

P

I

E

M

R

V

V

E

E

G

-

G

K

N

N

A

I

Y

Y

R

K

R

L

S

T

R

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

T

M

L

L

P

P

R

G

T

T

L

L

D

K

E

E

A

A

L

I

D

E

R

C

L

F

E

K

K

E

C

D

T

E

V

L

I

L

K

V

N

S

V

A

L

L

G

G

E

E

H

H

F

V

F

S

E

Q

A

S

F

I

T

I

M

N

I

V

K

A

R

M

V

A

E

D

L

W

D

D

A

S

F

Y

Q

R

D

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

L

N

D

H

R

L

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ A136), E170 (≠ D198), F185 (= F213), K186 (≠ L214), Y187 (≠ H215), N233 (≠ H261), S235 (= S263), S315 (≠ N343), R317 (= R345)
binding magnesium ion: E119 (= E138), H231 (= H259), E319 (= E347)

8ooxB Glutamine synthetase (see paper)
31% identity, 83% coverage: 74:447/450 of query aligns to 62:435/438 of 8ooxB

query
sites
8ooxB

D

E

P

S

D

D

A

M

D

V

L

L

R

R

P

P

D

D

F

P

R

D

T

T

L

F

C

R

V

V

A

L

P

P

-

W

G

S

Y

G

R

N

T

T

P

A

T

E

A

A

F

R

V

L

V

I

V

C

D

D

A

I

Y

E

K

L

S

P

N

D

G

G

I

K

P

P

L

F

D

M

L

G

S

C

P

P

R

R

Q

Q

V

V

L

L

K

K

R

K

V

N

L

M

A

E

L

E

Y

A

S

A

E

K

Q

L

G

G

W

Y

K

V

P

M

V

N

V

T

A

G

P

P

E

E

I

M

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

-

N

-

A

R

D

Q

P

D

D

G

L

M

P

P

L

T

S

N

P

I

P

P

A

Q

G

D

R

R

S

G

G

G

R

Y

P

F

E

D

T

L

S

A

P

P

Q

-

P

-

Y

-

G

-

L

-

E

-

A

-

I

I

T

D

E

L

F

A

E

E

D

E

L

I

I

K

E

R

N

E

I

I

Y

V

E

L

H

V

A

L

E

E

I

E

A

M

G

G

L

F

Q

E

I

V

D

E

T

A

L

A

I

H

H

H

E
|
E

A

V

G

A

A

F

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

D

D

N

P

A

I

L

T

A

L

T

S

A

D

D

Q

N

V

V

V

I

V

T

F

L

K

K

R

Y

L

V

V

A

R

K

Q

T

V

L

A

A

L

L

K

Q

H

H

G

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

I

A

F

D

G

Q

V

P

N

G

G

S

S

A

G

M

M

H

H

I

T

H

N

Q

T

S

S

L

L

V

F

E

K

I

-

G

-

T

D

G

G

K

K

N

N

L

A

F

F

V

Y

R

D

D

P

D

D

G

A

S

P

D

D

-

Q

-

I

S

S

P

D

M

T

F

L

R

R

H

Y

Y

F

I

V

G

G

L

V

Q

L

A

K

L

H

L

I

A

R

Q

A

V

I

T

T

P

A

L

I

F

T

A

N

P

P

N

L

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

P

H

G

H

Y

S

E

A

A

P

P

I

V

N

Y

L

I

E

T

C

W

G

S

Y

G

D

P

N

N

R

R

S

S

C

S

G

L

L

I

R

R

V

V

P

P

L

A

S

P

D

R

P

G

A

N

N

S

R

T

R

R

L

I

E

E

N

I

R

R

L

S

P

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

S

I

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

I

V

M

K

E

N

R

K

I

I

E

E

P

P

S

P

I

E

M

R

V

V

E

E

G

-

G

K

N

N

A

I

Y

Y

R

K

R

L

S

T

R

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

T

M

L

L

P

P

R

G

T

T

L

L

D

K

E

E

A

A

L

I

D

E

R

C

L

F

E

K

K

E

C

D

T

E

V

L

I

L

K

V

N

S

V

A

L

L

G

G

E

E

H

H

F

V

F

S

E

Q

A

S

F

I

T

I

M

N

I

V

K

A

R

M

V

A

E

D

L

W

D

D

A

S

F

Y

Q

R

D

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

L

N

D

H

R

L

Y

L

L

binding magnesium ion: E129 (= E140), E183 (= E203), E190 (= E210)

8wwvA Glutamine synthetase
29% identity, 95% coverage: 9:437/450 of query aligns to 24:466/490 of 8wwvA

query
sites
8wwvA

I

I

N

D

E

S

H

L

G

G

I

L

A

E

E

M

V

I

E

R

C

L

L

S

L

W

S

A

D

D

M

Q

N

Y

G

G

I

L

V

L

R

R

G

G

K

K

V

S

L

L

P

T

A

V

R

A

K

S

F

L

L

K

R

S

S

A

L

F

E

K

D

E

N

G

S

S

L

E

-

V

-

A

-

I

-

G

-

P

-

F

-

D

R

L

I

V

P

S

S

S

S

M

V

V

F

F

-

N

L

L

V

F

T

T

V

T

T

A

G

G

E

D

Y

F

P

E

F

D

A

E

D

L

D

S

N

G

N

N

T

P

V

T

V

V

I

V

D

-

P

-

D

-

A

-

D

-

L

M

R

V

P

P

D

D

F

P

R

T

T

T

L

F

C

K

V

V

A

L

P

P

G

-

Y

W

R

A

T

D

P

K

T

T

A

G

F

W

V

M

V

L

V

A

D

D

A

L

Y

H

K

W

S

K

N

S

G

G

I

E

P

P

L

F

D

P

L

L

S

C

P

P

R

R

Q

G

V

I

L

M

K

K

R

K

V

A

L

V

A

K

L

S

Y

L

S

S

E

D

Q

E

G

G

W

Y

K

L

P

F

V

K

V

C

A
x
G

P

I

E
|
E

I

L

E
|
E

F

W

Y

Y

L

L

T

T

Q

K

V

I

N

-

A

-

D

-

P

V

D

D

L

R

P

S

L

L

S

S

P

P

-

E

-

S

-

L

-

G

-

A

P

P

A

G

G

V

R

Q

S

P

G

D

R

A

P

I

E

Q

T

V

S

Q

P

P

Q

V

P

A

Y

Q

G

G

L

Y

E

S

A

V

I

L

T

L

E

E

F

H

E

H

-

L

D

D

L

Q

I

V

E

D

N

D

I

I

Y

M

E

S

H

K

A

V

E

R

I

K

A

G

G

L

L

L

Q

E

I

L

D

N

-

L

-

P

-

L

-
x
R

T

S

L

I

I

E

H

D

E
|
E

A

W

G

A

A

P

A

S

Q

Q

L

M

E
|
E

I

T

N

T

F
|
F

L
x
D

H
x
V

G

M

D

E

P

G

I

L

T

E

L

A

S

A

D

D

Q

A

V

A

V

L

F

I

K

K

R

S

L

A

V

I

R

K

Q

Q

V

I

A

C

L

S

K

R

H

H

G

G

V

Y

Y

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

M

A

A

I

D

N

Q

G

-

F

P

F

G
x
A

S

S

A

G

M

W

H
|
H

I

M

H
|
H

Q

Q

S
|
S

L

L

V

V

E

D

I

K

G

D

T

T

G

R

K

K

N

N

L

L

F

F

V

I

R

P

D

S

D

E

G

G

S

E

D

V

-

L

S

S

P

P

M

L

F

G

R

R

H

A

Y

Y

I

A

G

G

L

L

Q

L

A

A

L

N

L

G

A

S

Q

A

V

A

T

S

P

S

L

F

F

T

A

T

P

P

N

T

I

V

N

N

S

G

F

Y

R

R

R
|
R

M

R

R

Q

P

P

H

Y

H

S

S

L

A

A

P

P

I

D

N

R

L

R

E

A

C

W

G

A

Y

K

D

D

N

N

R
x
K

S

A

C

A

G

M

L

V

R

R

V

V

-

V

-

S

P

A

L

T

S

G

D

D

P

P

A

A

N

S

R

-

R
|
R

L

I

E
|
E

N

N

R

R

L

I

P

G

G

E

A

P

D

G

A

A

N

N

P

P

Y

Y

L

L

A

Y

M

M

A

A

A

S

S

Q

L

I

A

V

C

S

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

K

E

D

P

P

S

G

I

E

M

L

V

Q

E

E

G

-

N

S

A

P

Y

Y

-

D

R

A

R

Q

S

V

R

P

T

M

L

L

P

P

R

T

T

T

L

L

D

A

E

E

A

A

L

L

D

D

R

A

L

L

E

E

-

H

K

D

C

S

T

E

V

L

I

F

K

R

N

S

V

C

L

F

G

G

E

D

H

T

F

F

F

I

E

K

A

Y

F

W

T

L

M

Q

I

L

K

R

R

R

V

S

E

E

L

W

D

A

A

R

F

F

Q

L

D

D

V

A

binding adenosine-5'-diphosphate: G155 (≠ A136), E157 (= E138), R224 (vs. gap), F239 (= F213), D240 (≠ L214), V241 (≠ H215), H288 (= H261), S290 (= S263), R374 (= R345), E376 (= E347)
binding magnesium ion: E157 (= E138), E236 (= E210)
binding manganese (ii) ion: E157 (= E138), E159 (= E140), E229 (= E203), E236 (= E210), H286 (= H259), E376 (= E347)
binding l-methionine-s-sulfoximine phosphate: E157 (= E138), E159 (= E140), E229 (= E203), E236 (= E210), A282 (≠ G255), H286 (= H259), R340 (= R312), K358 (≠ R330)

8wwuB Glutamine synthetase
29% identity, 95% coverage: 9:437/450 of query aligns to 26:468/492 of 8wwuB

query
sites
8wwuB

I

I

N

D

E

S

H

L

G

G

I

L

A

E

E

M

V

I

E

R

C

L

L

S

L

W

S

A

D

D

M

Q

N

Y

G

G

I

L

V

L

R

R

G

G

K

K

V

S

L

L

P

T

A

V

R

A

K

S

F

L

L

K

R

S

S

A

L

F

E

K

D

E

N

G

S

S

L

E

-

V

-

A

-

I

-

G

-

P

-

F

-

D

R

L

I

V

P

S

S

S

S

M

V

V

F

F

-

N

L

L

V

F

T

T

V

T

T

A

G

G

E

D

Y

F

P

E

F

D

A

E

D

L

D

S

N

G

N

N

T

P

V

T

V

V

I

V

D

-

P

-

D

-

A

-

D

-

L

M

R

V

P

P

D

D

F

P

R

T

T

T

L

F

C

K

V

V

A

L

P

P

G

-

Y

W

R

A

T

D

P

K

T

T

A

G

F

W

V

M

V

L

V

A

D

D

A

L

Y

H

K

W

S

K

N

S

G

G

I

E

P

P

L

F

D

P

L

L

S

C

P

P

R

R

Q

G

V

I

L

M

K

K

R

K

V

A

L

V

A

K

L

S

Y

L

S

S

E

D

Q

E

G

G

W

Y

K

L

P

F

V

K

V

C

A
x
G

P

I

E
|
E

I

L

E
|
E

F

W

Y

Y

L

L

T

T

Q

K

V

I

N

-

A

-

D

-

P

V

D

D

L

R

P

S

L

L

S

S

P

P

-

E

-

S

-

L

-

G

-

A

P

P

A

G

G

V

R

Q

S

P

G

D

R

A

P

I

E

Q

T

V

S

Q

P

P

Q

V

P

A

Y

Q

G

G

L

Y

E

S

A

V

I

L

T

L

E

E

F

H

E

H

-

L

D

D

L

Q

I

V

E

D

N

D

I

I

Y

M

E

S

H

K

A

V

E

R

I

K

A

G

G

L

L

L

Q

E

I

L

D

N

-

L

-

P

-

L

-
x
R

T

S

L

I

I

E

H

D

E
|
E

A

W

G

A

A

P

A

S

Q

Q

L

M

E
|
E

I

T

N

T

F
|
F

L

D

H
x
V

G

M

D

E

P

G

I

L

T

E

L

A

S

A

D

D

Q

A

V

A

V

L

F

I

K

K

R

S

L

A

V

I

R

K

Q

Q

V

I

A

C

L

S

K

R

H

H

G

G

V

Y

Y

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

M

A

A

I

D

N

Q

G

-

F

P

F

G

A

S

S

A

G

M

W

H
|
H

I

M

H
|
H

Q

Q

S
|
S

L

L

V

V

E

D

I

K

G

D

T

T

G

R

K

K

N

N

L

L

F

F

V

I

R

P

D

S

D

E

G

G

S

E

D

V

-

L

S

S

P

P

M

L

F

G

R

R

H

A

Y

Y

I

A

G

G

L

L

Q

L

A

A

L

N

L

G

A

S

Q

A

V

A

T

S

P

S

L

F

F

T

A

T

P

P

N

T

I

V

N

N

S

G

F

Y

R

R

M

R

R

Q

P

P

H

Y

H

S

S

L

A

A

P

P

I

D

N

R

L

R

E

A

C

W

G

A

Y

K

D

D

N

N

R
x
K

S

A

C

A

G

M

L

V

R
|
R

V

V

-

V

-

S

P

A

L

T

S

G

D

D

P

P

A

A

N

S

R

-

R
|
R

L

I

E
|
E

N

N

R

R

L

I

P

G

G

E

A

P

D

G

A

A

N

N

P

P

Y

Y

L

L

A

Y

M

M

A

A

A

S

S

Q

L

I

A

V

C

S

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

K

E

D

P

P

S

G

I

E

M

L

V

Q

E

E

G

-

N

S

A

P

Y

Y

-

D

R

A

R

Q

S

V

R

P

T

M

L

L

P

P

R

T

T

T

L

L

D

A

E

E

A

A

L

L

D

D

R

A

L

L

E

E

-

H

K

D

C

S

T

E

V

L

I

F

K

R

N

S

V

C

L

F

G

G

E

D

H

T

F

F

F

I

E

K

A

Y

F

W

T

L

M

Q

I

L

K

R

R

R

V

S

E

E

L

W

D

A

A

R

F

F

Q

L

D

D

V

A

binding phosphoaminophosphonic acid-adenylate ester: G157 (≠ A136), E159 (= E138), R226 (vs. gap), F241 (= F213), V243 (≠ H215), H290 (= H261), S292 (= S263), K360 (≠ R330), R365 (= R335), R376 (= R345)
binding magnesium ion: E159 (= E138), E238 (= E210)
binding manganese (ii) ion: E159 (= E138), E161 (= E140), E231 (= E203), E238 (= E210), H288 (= H259), E378 (= E347)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 84% coverage: 72:448/450 of query aligns to 62:446/446 of A0R083

query
sites
A0R083

V

V

I

F

D

E

P

S

D

D

A

T

D

V

L

A

R

R

P

P

D

D

F

P

R

S

T

T

L

F

C

Q

V

V

A

L

P

P

G

-

Y

W

R

K

T

T

P

S

-

D

-

G

-

N

-

H

-

Y

T

S

A

A

F

R

V

M

V

F

V

C

D

D

A

I

Y

T

K

M

S

P

N

D

G

G

I

S

P

P

L

S

D

W

L

A

S

D

P

S

R

R

Q

H

V

V

L

L

K

R

R

R

V

Q

L

L

A

A

L

K

Y

A

S

S

E

D

Q

L

G

G

W

F

K

T

P

C

V

Y

V

V

A

H

P

P

E

E

I

I

E

E

F

F

Y

F

L

L

T

L

Q

K

V

P

N

G

A

P

D

N

P

D

D

G

L

T

P

P

L

-

S

P

P

E

P

P

A

A

G

D

R

N

S

G

G

G

R

-

P

-

E

-

T

-

S

-

P

-

Q

-

P

-

Y

Y

G

F

L

D

E

Q

A

A

I

V

T

-

E

-

F

-

E

H

D

D

L

A

I

A

E

P

N

N

I

F

Y

R

E

R

H

H

A

A

-

I

-

E

-

A

-

L

E

E

I

Q

A

M

G

G

L

I

Q

S

I

V

D

E

T

F

L

S

I

H

H

H

E

E

A

G

G

A

A

P

A

G

Q

Q

L

Q

E

E

I

I

N

D

F

L

L

R

H

Y

G

A

D

D

P

A

I

L

T

S

L

M

S

A

D

D

Q

N

V

V

V

M

V

T

F

F

K

R

R

Y

L

L

V

V

R

K

Q

E

V

V

A

A

L

L

K

A

H

D

G

G

V

V

Y

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

F

A

A

D

E

Q

H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

Q

M

S

S

L

L

V

F

E

E

I

G

G

D

T

T

G

N

K

A

N

F

L

H

F

S

V

P

R

D

D

D

D

P

G

L

S

Q

D

L

S

S

P

D

M

V

F

A

R

K

H

S

Y

F

I

I

G

A

G

G

L

I

Q

L

A

E

L

H

L

A

A

N

Q

E

V

I

T

S

P

A

L

V

F

T

A

N

P

Q

N

W

I

V

N

N

S

S

F

Y

R

K

R

R

M

L

R

V

P

H

H

G

S

E

A

A

P

P

I

T

N

A

L

A

E

S

C

W

G

G

Y

A

D

A

N

N

R

R

S

S

C

A

G

L

L

V

R

R

V

V

P

P

L

M

S

Y

D

T

P

P

-

H

-

K

-

V

A

S

N

S

R

R

L

V

E

E

N

V

R

R

L

S

P

P

G

D

A

S

D

A

A

C

N

N

P

P

Y

Y

L

L

A

T

M

F

A

A

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

R

G

G

I

V

M

E

E
x
K

R

G

I

Y

E

V

P

L

S

G

I

P

M

Q

V

A

E

E

G

D

G

N

-

V

-

W

-

S

-

L

-

T

N

Q

A

E

Y

E

R

R

S

A

-

M

-

G

-

Y

R

R

T

E

L

L

P

P

R

T

T

S

L

L

D

G

E

N

A

A

L

L

D

E

R

S

L

M

E

E

K

N

C

S

T

E

V

L

I

V

K

A

N

E

V

A

L

L

G

G

E

E

H

H

F

V

F

F

E

D

A

Y

F

F

T

L

M

R

I

N

K

K

R

R

V

S

E

E

L

W

D

E

A

N

F

Y

Q

R

D

S

V

H

I

V

S

T

S

P

W

Y

E

E

-

L

R

K

N

N

H

Y

L

L

L

S

L

L

K363 (≠ E374) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tdvC Glutamine synthetase (see paper)
28% identity, 98% coverage: 8:447/450 of query aligns to 11:440/443 of 7tdvC

query
sites
7tdvC

W

F

I

V

N

E

E

E

H

E

G

N

I

V

A

R

E

Y

V

L

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

V

P

P

A

V

R

S

K

Q

F

L

L

E

R

K

S

V

L

L

E

D

D

N

N

E

S

M

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N

F

T

V

V

R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

H

P

P

D

D

F

L

R

D

T

T

L

W

C

V

V

I

A

F

P

P

-

W

-

T

G

A

Y

G

R

Q

T

G

P

K

T

V

A

A

F

R

V

L

V

I

V

C

D

D

A

V

Y

Y

K

K

S

T

N

D

G

G

I

T

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

A

V

N

L

L

K

K

R

R

V

V

L

L

A

K

L

E

Y

M

S

E

E

D

Q

L

G

G

W

F

K

T

P

D

V

F

-

N

V

L

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

-

E

-

K

A

G

D

E

P

P

D

T

L

L

P

E

L

L

S

N

P

D

A

G

G

G

R

Y

S

F

G

-

R

-

P

-

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

D

A

M

G

G

L

F

Q

D

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

F
|
F

L
x
K

H
x
Y

G

A

D

D

P

A

I

V

T

T

L

A

S

C

D

D

Q

N

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

N

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H
x
N

Q
x
V

S
|
S

L

L

V

F

E

K

I

-

G

-

T

-

G

G

K

K

N

E

L

N

F

A

V

F

R

F

D

D

D

P

G

N

S

T

D

E

S

M

P

G

M

L

F

T

R

E

-

T

-

A

-

Y

H

Q

Y

F

I

T

G

A

G

G

L

V

Q

L

A

K

L

N

L

A

A

R

Q

G

V

F

T

T

P

A

L

V

F

C

A

N

P

P

N

L

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H

G

H

Y

S
x
E

A

A

P

P

I

C

N

Y

L

I

E

A

C

W

G

S

Y

G

D

K

N

N

R
|
R

S

S

C

P

G

L

L

I

R
|
R

V

V

P

P

L

S

S

S

D

R

P

G

A

L

N
x
S

R

T

R
|
R

L

I

E
|
E

N

V

R

R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

M

L

A

A

S

I

L

L

A

E

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

L

E

K

P

V

S

P

I

E

M

P

V

V

E

-

G

N

G

Q

N

N

A

I

Y

Y

R

E

R

M

S

N

R

R

T

E

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

R

S

T

T

L

L

D

Y

E

T

A

A

L

L

D

K

R

A

L

M

E

R

K

E

C

N

T

E

V

V

I

I

K

K

N

K

V

A

L

L

G

G

E

N

H

H

F

I

F

Y

E

N

A

Q

F

F

T

I

M

N

I

S

K

K

R

S

V

I

E

E

L

W

D

D

A

Y

F

Y

Q

R

D

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

N

D

H

Q

L

Y

L

M

binding adenosine-5'-diphosphate: G129 (≠ A136), E131 (= E138), E183 (≠ D198), D197 (≠ N212), F198 (= F213), K199 (≠ L214), Y200 (≠ H215), N246 (≠ H261), V247 (≠ Q262), S248 (= S263), R320 (= R335), S328 (≠ N343), R330 (= R345)
binding magnesium ion: E131 (= E138), E131 (= E138), E133 (= E140), E188 (= E203), E195 (= E210), E195 (= E210), H244 (= H259), E332 (= E347)
binding l-methionine-s-sulfoximine phosphate: E131 (= E138), E133 (= E140), E188 (= E203), E195 (= E210), G240 (= G255), H244 (= H259), R297 (= R312), E303 (≠ S318), R315 (= R330)

7tf6A Glutamine synthetase (see paper)
28% identity, 97% coverage: 11:447/450 of query aligns to 13:435/438 of 7tf6A

query
sites
7tf6A

E

E

H

E

G

N

I

V

A

R

E

Y

V

L

E

R

C

L

L

Q

L

F

S

T

D

D

M

I

N

L

G

G

I

T

V

I

R

K

G

N

K

V

V

E

L

V

P

P

A

V

R

S

K

Q

F

L

L

E

R

K

S

V

L

L

E

D

D

N

N

E

S

M

L

M

R

F

I

D

P

G

S

S

V

I

F

-

L

-

V

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

F

-

A

-

D

-

D

E

N

G

N
x
F

T

V

V
x
R

V

I

I

E

D

E

P

S

D

D

A

M

D

Y

L

L

R

H

P

P

D

D

F

L

R

D

T

T

L

W

C

V

V

I

A

F

P

P

G

W

Y

G

R

K

T

V

P

-

T

-

A

A

F

R

V

L

V

I

V

C

D

D

A

V

Y

Y

K

K

S

T

N

D

G

G

I

T

P

P

L

F

D

E

L

G

S

D

P

P

R

R

Q

A

V

N

L

L

K

K

R

R

V

V

L

L

A

K

L

E

Y

M

S

E

E

D

Q

L

G

G

W

F

K

T

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

V

L

N

D

-

E

-

K

A

G

D

E

P

P

D

T

L

L

P

E

L

L

S

N

P

D

A

G

G

G

R

Y

S

F

G

-

R

-

P

-

E

D

T

L

S

A

P

P

Q

T

P

D

Y

L

G

G

L

-

E

-

A

-

I

-

T

-

E

-

F

-

E

E

D

N

L

C

I

R

E

R

N

D

I

I

Y

V

E

L

H

E

A

L

E

E

I

D

A

M

G

G

L

F

Q

D

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

F

F

L

K

H

Y

G

A

D

D

P

A

I

V

T

T

L

A

S

C

D

D

Q

N

V

I

V

Q

V

T

F

F

K

K

R

L

L

V

V

V

R

K

Q

T

V

I

A

A

L

R

K

K

H

H

G

N

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

A

F

D
x
G

Q

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H

N

Q

V

S

S

L

L

V

F

E

K

I

-

G

-

T

-

G

G

K

K

N

E

L

N

F

A

V

F

R

F

D

D

D

P

G

N

S

T

D

E

S

M

P

G

M

L

F

T

R

E

-

T

-

A

-

Y

H

Q

Y

F

I

T

G

A

G

G

L

V

Q

L

A

K

L

N

L

A

A

R

Q

G

V

F

T

T

P

A

L

V

F

C

A

N

P

P

N

L

I

V

N

N

S

S

F

Y

R

K

R
|
R

M

L

R

V

P

P

H
x
G

H
x
Y

S
x
E

A

A

P

P

I

C

N

Y

L

I

E

A

C

W

G

S

Y

G

D

K

N

N

R
|
R

S

S

C

P

G

L

L

I

R

R

V

V

P

P

L

S

S

S

D

R

P

G

A

L

N

S

R

T

R

R

L

I

E
|
E

N

V

R

R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

M

L

A

A

S

I

L

L

A

E

C

A

G

G

Y

L

I

D

G

G

I

I

M

K

E

N

R

K

I

L

E

K

P

V

S

P

I

E

M

P

V

V

E

-

G

N

G

Q

N

N

A

I

Y
|
Y

R

E

R

M

S

N

R

R

T

E

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

R

S

T

T

L

L

D

Y

E

T

A

A

L

L

D

K

R

A

L

M

E

R

K

E

C

N

T

E

V

V

I

I

K

K

N

K

V

A

L

L

G

G

E

N

H

H

F

I

F

Y

E

N

A

Q

F

F

T

I

M

N

I

S

K

K

R

S

V

I

E

E

L

W

D

D

A
x
Y

F

Y

Q

R

D

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

N

D

H
x
Q

L

Y

L

M

binding glutamine: E128 (= E140), E183 (= E203), G235 (= G255), H239 (= H259), R292 (= R312), E298 (≠ S318)
binding magnesium ion: E126 (= E138), E128 (= E140), E183 (= E203), E190 (= E210), H239 (= H259), E327 (= E347)
binding : F58 (≠ N69), R60 (≠ V71), G232 (≠ D252), N234 (≠ P254), G296 (≠ H316), Y297 (≠ H317), R310 (= R330), Y367 (= Y388), Y421 (≠ A433), Q433 (≠ H445)

Sites not aligning to the query:

binding : 437

Query Sequence

>WP_011389007.1 NCBI__GCF_000013085.1:WP_011389007.1
MINEFEAWINEHGIAEVECLLSDMNGIVRGKVLPARKFLRSLEDNSLRIPSSVFLVTVTG
EYPFADDNNTVVIDPDADLRPDFRTLCVAPGYRTPTAFVVVDAYKSNGIPLDLSPRQVLK
RVLALYSEQGWKPVVAPEIEFYLTQVNADPDLPLSPPAGRSGRPETSPQPYGLEAITEFE
DLIENIYEHAEIAGLQIDTLIHEAGAAQLEINFLHGDPITLSDQVVVFKRLVRQVALKHG
VYATFMAKPMADQPGSAMHIHQSLVEIGTGKNLFVRDDGSDSPMFRHYIGGLQALLAQVT
PLFAPNINSFRRMRPHHSAPINLECGYDNRSCGLRVPLSDPANRRLENRLPGADANPYLA
MAASLACGYIGIMERIEPSIMVEGGNAYRRSRTLPRTLDEALDRLEKCTVIKNVLGEHFF
EAFTMIKRVELDAFQDVISSWERNHLLLKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory