SitesBLAST

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
39% identity, 97% coverage: 1:180/185 of query aligns to 2:179/185 of 3nz2J

query
sites
3nz2J

M

M

P

K

A

M

S

S

E

E

R

L

E

E

K

K

M

M

A

L

A

K

G

G

E

E

W

H

Y

F

C

D

C

G

L

A

D

S

H

A

E

E

L

I

D

E

I

A

L

L

R

R

R

S

Q

Q

A

A

-

G

R

R

V

L

A

K

V

L

H

E

A

I

H

N

N

Q

S

S

L

L

P

D

P

E

D

A

E

E

R

R

G

Y

A

A

V

L

A

Q

P

-

A

-

L

-

R

R

R

E

L

L

F

F

A

G

E

H

A

L

A

G

P

H

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K

V

S

F

C

I

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E

Q

A

P

P

P

F

F

H

H

C

C

S

E

Y

F

G

G

I

K

N

T

I

I

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R

L

I

G

G

E

D

R

H

V

T

Y

F

F

I

N

N

A

M

G

N

C

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T

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I

M

L

L

D

D

S

G

G

A

S

P

V

I

T

T

I

I

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G

D

D

R

H

T

V

M

L

L

I

G
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G

P

P

G

S

V

T

Q

Q

I

F

Y
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Y

C

T

A

A

E

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H
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H

H

S

K

L

D

D

P

Y

A

R

L

R

R

R

S

Q

E

A

G

W

I

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I

I

A

C

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K

P

P

V

I

A

V

I

I

G

E

S

D

D

D

V

V

W
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W

I

I

G
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G

G

G

G

N

T

V

I

V

I

I

L

N

G

Q

G

G

V

V

T

T

I

I

G

G

D

A

G

R

A

S

I

V

V

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
N

K

Q

D

D

V

V

P

P

A

P

G

D

A

T

R

L

V

V

V
x
G

G

G

N
x
T

P
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P

A

A

R

R

binding acetyl coenzyme *a: G104 (= G105), Y110 (= Y111), H114 (= H115), W138 (= W139), G140 (= G141), A158 (≠ G159), A159 (= A160), N164 (≠ T165), G174 (≠ V175), T176 (≠ N177), P177 (= P178)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
39% identity, 96% coverage: 4:180/185 of query aligns to 2:176/183 of 3nz2C

query
sites
3nz2C

S

S

E

E

R

L

E

E

K

K

M

M

A

L

A

K

G

G

E

E

W

H

Y

F

C

D

C

G

L

A

D

S

H

A

E

E

L

I

D

E

I

A

L

L

R

R

R

S

Q

Q

A

A

-

G

R

R

V

L

A

K

V

L

H

E

A

I

H

N

N

Q

S

S

L

L

P

D

P

E

D

A

E

E

R

R

G

Y

A

A

V

L

A

Q

P

-

A

-

L

-

R

R

R

E

L

L

F

F

A

G

E

H

A

L

A

G

P

H

E

K

V

S

F

C

I

V

E

Q

A

P

P

P

F

F

H

H

C

C

S

E

Y

F

G

G

I

K

N

T

I

I

V

R

L

I

G

G

E

D

R

H

V

T

Y

F

F

I

N

N

A

M

G

N

C

V

T

V

I

M

L

L

D

D

S

G

G

A

S

P

V

I

T

T

I

I

G

G

D

D

R

H

T

V

M

L

L

I

G

G

P

P

G

S

V

T

Q

Q

I

F

Y
|
Y

C

T

A

A

E

S

H
|
H

H

S

K

L

D

D

P

Y

A

R

L

R

R

R

S

Q

E

A

G

W

I

E

E

T

I

I

A

C

R

K

P

P

V

I

A

V

I

I

G

E

S

D

D

D

V

V

W

W

I

I

G
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G

G

G

G

N

T

V

I

V

I

I

L

N

G

Q

G

G

V

V

T

T

I

I

G

G

D

A

G

R

A

S

I

V

V

V

G
x
A

A
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A

G
x
N

A

S

V

V

T
x
N

K

Q

D

D

V

V

P

P

A

P

G

D

A

T

R

L

V

V

V
x
G

G

G

N
x
T

P
|
P

A

A

R

R

binding acetyl coenzyme *a: Y107 (= Y111), H111 (= H115), G137 (= G141), A155 (≠ G159), A156 (= A160), N161 (≠ T165), G171 (≠ V175), T173 (≠ N177), P174 (= P178)
binding magnesium ion: N157 (≠ G161), T173 (≠ N177)

Sites not aligning to the query:

binding acetyl coenzyme *a: 178, 179

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
38% identity, 94% coverage: 7:180/185 of query aligns to 2:169/176 of 3ectA

query
sites
3ectA

E

E

K

K

M

M

A

L

A

K

G

G

E

H

W

A

Y

S

C

A

C

-

L

-

D

-

H

-

E

E

L

I

D

E

I

A

L

L

R

R

R

S

Q

Q

A

A

-

G

R

R

V

L

A

K

V

L

H

E

A

I

H

N

N

Q

S

S

L

L

P

D

P

E

D

A

E

E

R

R

G

Y

A

A

V

L

A

Q

P

-

A

-

L

-

R

R

R

E

L

L

F

F

A

G

E

H

A

L

A

G

P

H

E

K

V

S

F

C

I

V

E

Q

A

P

P

P

F

F

H

H

C

C

S

E

Y

F

G

G

I

K

N

T

I

I

V

R

L

I

G

G

E

D

R

H

V

T

Y

F

F

I

N

N

A

M

G

N

C

V

T

V

I

M

L

L

D

D

S

G

G

A

S

P

V

I

T

T

I

I

G

G

D

D

R

H

T

V

M

L

L

I

G

G

P

P

G

S

V

T

Q

Q

I

F

Y

Y

C

T

A

A

E

S

H

H

H

S

K

L

D

D

P

Y

A

R

L

R

R

R

S

Q

E

A

G

W

I

E

E

T

I

I

A

C

R

K

P

P

V

I

A

V

I

I

G

E

S

D

D

D

V

V

W

W

I

I

G

G

G

N

T

V

I

V

I

I

L

N

G

Q

G
|
G

V

V

T

T

I

I

G

G

D

A

G

R

A

S

I

V

V

V

G

A

A

A

G

N

A

S

V

V

T

N

K

Q

D
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D

V

V

P

P

A

P

G

D

A

T

R

L

V

V

V

G

G

G

N

T

P

P

A

A

R

R

binding calcium ion: G138 (= G149), D156 (= D167)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 100% coverage: 1:185/185 of query aligns to 3:185/203 of P07464

query
sites
P07464

M

M

P

P

A

M

S

T

E

E

R

R

E

-

K

-

M

I

A

R

A

A

G

G

E

K

W

L

Y

F

C

T

C
x
D

L

M

D

C

H

E

E

G

L

L

D

P

I

E

L

K

R

R

R

L

Q

R

A

G

R

K

V

T

A

L

V

M

H

Y

A

E

H

F

N

N

S

H

L

S

P

H

P

P

D

S

E

E

R

V

G

E

A

K

V

R

A

E

P

S

A

L

L

I

R

K

R

E

L

M

F

F

A

A

E

T

A

V

A

G

P

E

E

N

V

A

F

W

I

V

E

E

A

P

P

P

F

V

H

Y

C

F

S
|
S

Y

Y

G

G

I

S

N

N

I

I

V

H

L

I

G

G

E

R

R

N

V

F

Y

Y

F

A

N
|
N

A

F

G

N

C

L

T

T

I

I

L

V

D

D

S
x
D

G

Y

S

T

V

V

T

T

I

I

G

G

D

D

R

N

T

V

M

L

L

I

G

A

P

P

G

N

V

V

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T

I

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Y

S

C

V

A

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E

G

H
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H

H

P

K

V

D

H

P

H

A

E

L

L

R

R

S

K

E

N

G

G

I

E

E

M

I

Y

A

S

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F

P

P

V

I

A

T

I

I

G

G

S

N

D

N

V

V

W

W

I

I

G

G

G
x
S

G

H

T

V

I

V

I

I

L

N

G

P

G

G

V

V

T

T

I

I

G

G

D

D

G

N

A

S

I

V

V

I

G

G

A
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A

G

G

A

S

V

I

V

V

T
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T

K
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K

D

D

V

I

P

P

A

P

G

N

A

V

R

V

V

A

G

G

N

V

P

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C

R
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R

P

V

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N
x
R

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E

V

I

D17 (≠ C17) binding substrate
S71 (= S71) binding substrate
N85 (= N85) binding in other chain; binding in other chain
D93 (≠ S93) binding substrate
H115 (= H115) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ G142) binding in other chain
A160 (= A160) binding in other chain
TK 165:166 (= TK 165:166) binding acetyl-CoA
R180 (= R180) binding acetyl-CoA
R183 (≠ N183) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
34% identity, 100% coverage: 1:185/185 of query aligns to 2:184/201 of 1krvA

query
sites
1krvA

M

M

P

P

A

M

S

T

E

E

R

R

E

-

K

-

M

I

A

R

A

A

G

G

E

K

W

L

Y

F

C

T

C
x
D

L

M

D

C

H

E

E

G

L

L

D

P

I

E

L

K

R
|
R

R

L

Q

R

A

G

R

K

V

T

A

L

V

M

H

Y

A

E

H

F

N

N

S

H

L

S

P

H

P

P

D

S

E

E

R

V

G

E

A

K

V

R

A

E

P

S

A

L

L

I

R

K

R

E

L

M

F

F

A

A

E

T

A

V

A

G

P

E

E

N

V

A

F

W

I

V

E

E

A

P

P

P

F

V

H

Y

C

F

S
|
S

Y

Y

G

G

I

S

N

N

I

I

V

H

L

I

G

G

E

R

R

N

V

F

Y
|
Y

F

A

N
|
N

A

F

G

N

C

L

T

T

I

I

L
x
V

D

D

S
x
D

G

Y

S

T

V

V

T

T

I

I

G

G

D

D

R

N

T

V

M

L

L

I

G
x
A

P

P

G

N

V

V

Q

T

I

L

Y
x
S

C

V

A
x
T

E

G

H

H

H

P

K

V

D

H

P

H

A

E

L

L

R

R

S

K

E

N

G

G

I

E

E
x
M

I

Y

A

S

R

F

P

P

V

I

A

T

I

I

G

G

S

N

D

N

V

V

W
|
W

I

I

G
|
G

G
x
S

G

H

T

V

I

V

I

I

L
x
N

G

P

G

G

V

V

T

T

I

I

G

G

D

D

G

N

A

S

I

V

V

I

G

G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

G

N

A

V

R

V

V

A

G

G

N

V

P
|
P

A

C

R
|
R

P

V

I

I

N
x
R

R

E

V

I

binding 4-nitrophenyl beta-D-galactopyranoside: D16 (≠ C17), R25 (= R26), S70 (= S71), Y82 (= Y83), N84 (= N85), V90 (≠ L91), D92 (≠ S93), M126 (≠ E127)
binding coenzyme a: A104 (≠ G105), S110 (≠ Y111), T112 (≠ A113), W138 (= W139), G140 (= G141), S141 (≠ G142), N146 (≠ L147), A159 (= A160), P177 (= P178), R179 (= R180), R182 (≠ N183)

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
34% identity, 100% coverage: 1:185/185 of query aligns to 2:184/201 of 1kruA

query
sites
1kruA

M

M

P

P

A

M

S

T

E

E

R

R

E

-

K

-

M

I

A

R

A

A

G

G

E

K

W

L

Y

F

C

T

C
x
D

L

M

D

C

H

E

E

G

L

L

D
x
P

I

E

L

K

R
|
R

R

L

Q

R

A

G

R

K

V

T

A

L

V

M

H

Y

A

E

H

F

N

N

S

H

L

S

P

H

P

P

D

S

E

E

R

V

G

E

A

K

V

R

A

E

P

S

A

L

L

I

R

K

R

E

L

M

F

F

A

A

E

T

A

V

A

G

P

E

E

N

V

A

F
x
W

I

V

E

E

A

P

P

P

F

V

H

Y

C

F

S
|
S

Y

Y

G

G

I

S

N

N

I

I

V

H

L

I

G

G

E

R

R

N

V

F

Y
|
Y

F

A

N
|
N

A

F

G

N

C

L

T

T

I

I

L
x
V

D

D

S
x
D

G

Y

S

T

V

V

T

T

I

I

G

G

D

D

R

N

T

V

M
x
L

L

I

G

A

P

P

G

N

V

V

Q

T

I

L

Y

S

C

V

A

T

E

G

H
|
H

H

P

K

V

D

H

P

H

A

E

L

L

R

R

S

K

E

N

G

G

I

E

E
x
M

I

Y

A

S

R

F

P

P

V

I

A

T

I

I

G

G

S

N

D

N

V

V

W
|
W

I

I

G
|
G

G
x
S

G

H

T

V

I

V

I

I

L

N

G

P

G

G

V

V

T

T

I

I

G

G

D

D

G

N

A

S

I

V

V

I

G
|
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

G

N

A

V

R

V

V

A

G

G

N

V

P
|
P

A

C

R

R

P

V

I

I

N
x
R

R

E

V

I

binding coenzyme a: H114 (= H115), W138 (= W139), G140 (= G141), S141 (≠ G142), G158 (= G159), A159 (= A160), P177 (= P178), R182 (≠ N183)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (≠ C17), P22 (≠ D23), R25 (= R26), W62 (≠ F63), S70 (= S71), Y82 (= Y83), Y82 (= Y83), N84 (= N85), V90 (≠ L91), D92 (≠ S93), L102 (≠ M103), H114 (= H115), M126 (≠ E127)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
34% identity, 100% coverage: 1:185/185 of query aligns to 2:184/200 of 1krrA

query
sites
1krrA

M

M

P

P

A

M

S

T

E

E

R

R

E

-

K

-

M

I

A

R

A

A

G

G

E

K

W

L

Y

F

C

T

C

D

L

M

D

C

H

E

E

G

L

L

D

P

I

E

L

K

R

R

R

L

Q

R

A

G

R

K

V

T

A

L

V

M

H

Y

A

E

H

F

N

N

S

H

L

S

P

H

P

P

D

S

E

E

R

V

G

E

A

K

V

R

A

E

P

S

A

L

L

I

R

K

R

E

L

M

F

F

A

A

E

T

A

V

A

G

P

E

E

N

V

A

F

W

I

V

E

E

A

P

P

P

F

V

H

Y

C

F

S

S

Y

Y

G

G

I

S

N

N

I

I

V

H

L

I

G

G

E

R

R

N

V

F

Y

Y

F

A

N
|
N

A

F

G

N

C

L

T

T

I

I

L

V

D

D

S

D

G

Y

S

T

V

V

T

T

I

I

G

G

D

D

R

N

T

V

M

L

L
x
I

G
x
A

P
|
P

G

N

V

V

Q

T

I

L

Y

S

C

V

A
x
T

E

G

H
|
H

H

P

K

V

D

H

P

H

A

E

L

L

R

R

S

K

E

N

G

G

I

E

E

M

I

Y

A

S

R

F

P

P

V

I

A

T

I

I

G

G

S

N

D

N

V

V

W

W

I

I

G
|
G

G
x
S

G

H

T

V

I

V

I

I

L
x
N

G

P

G

G

V

V

T

T

I

I

G

G

D

D

G

N

A

S

I

V

V

I

G
|
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

G

N

A

V

R

V

V

A

V
x
A

G

G

N

V

P
|
P

A

C

R

R

P

V

I

I

N
x
R

R

E

V

I

binding acetyl coenzyme *a: N84 (= N85), I103 (≠ L104), A104 (≠ G105), P105 (= P106), T112 (≠ A113), H114 (= H115), G140 (= G141), S141 (≠ G142), N146 (≠ L147), G158 (= G159), A159 (= A160), A174 (≠ V175), P177 (= P178), R182 (≠ N183)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
33% identity, 65% coverage: 64:184/185 of query aligns to 157:279/307 of A1ADJ6

query
sites
A1ADJ6

I

I

E

K

A

G

P

D

F

I

H

V

C

A

S

T

Y

K

G

G

I

S

N

K

I

V

V

I

L

I

G

G

E

R

R

R

V

T

Y

T

F

I

N

G

A

A

G

G

C

F

T

E

I

V

L

V

-

T

D

D

S

K

G

C

S

N

V

V

T

T

I

I

G

G

D

H

R

D

T

C

M

M

L

I

G

A

P

R

G

D

V

V

Q

I

I

L

Y

R

C

A

A

S

E

D

H

G

H
|
H

K

P

D

I

P

F

A

D

L

I

R

H

S

S

E

K

G

K

-

R

I

I

E

N

I

W

A

A

R

K

P

D

V

I

A

I

I

I

G

S

S

S

D

Y

V

V

W
|
W

I

V

G

G

G

R

G

N

T

V

I

S

I

I

L

M

G

K

G

G

V

V

T

S

I

V

G

G

D

S

G

G

A

S

I

V

V

I

G

G

A

Y

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

V

P

P

A

S

G

M

A

C

R

A

V

A

G

G

N

N

P

P

A

A

R

K

P

I

I

I

N

K

R

R

H210 (= H116) mutation to A: Loss of activity.
W234 (= W139) mutation to A: Loss of activity.

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
38% identity, 57% coverage: 75:180/185 of query aligns to 46:139/294 of 4mzuF

query
sites
4mzuF

N

D

I

V

V

V

L

I

G

G

E

D

R

N

V

V

Y

T

F

I

N

K

A

S

G

G

C

V

T

Q

I

I

L

W

D

D

S

G

G

-

S

-

V

I

T

H

I

I

G

Q

D

D

R

D

T

V

M

F

L

I

G

G

P

P

G

N

V

V

Q

T

I

F

Y

T

C

-

A

-

E

-

H

-

K
x
N

D
|
D

P
x
K

A

Q

L

P

R

R

S

S

E

K

G

I

I

K

E

T

I

I

A

V

R

K

P

K

V

G

A

A

I

S

G

-

S

-

D

-

V

-

W

-

I

I

G
|
G

G
x
A

G

N

T

S

I

T

I

I

L
|
L

G

P

G

G

V

I

T

L

I

I

G

G

D

E

G

N

A

A

I

M

V

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

A

D

G

N

A

A

R

I

V

V

V
x
I

G

G

N
|
N

P

P

A

G

R
|
R

binding coenzyme a: N81 (≠ K117), D82 (= D118), K83 (≠ P119), G100 (= G141), A101 (≠ G142), L106 (= L147), G118 (= G159), A119 (= A160), T124 (= T165), K125 (= K166), I134 (≠ V175), N136 (= N177), R139 (= R180)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
38% identity, 49% coverage: 91:180/185 of query aligns to 42:138/290 of 4mzuB

query
sites
4mzuB

L

L

D

I

S

E

G

N

S

D

V

V

T

V

I

I

G

G

D

D

R

N

T

V

M

T

L

I

G

K

P

S

G
|
G

V

V

Q

Q

I

I

Y

W

C

D

A

G

E

I

H

H

H

I

K

Q

D

D

P

D

A

V

L
x
F

R

I

S

G

E

P

G
x
N

I

V

E

T

I

F

A
x
T

-
x
N

-
x
D

-
x
K

R

Q

P
|
P

V

R

A

S

I

L

G

K

S

T

D

I

V

V

W

K

-

K

-

G

-

A

-

S

I

I

G
|
G

G
x
A

G

N

T

S

I

T

I

I

L

L

G

P

G

G

V

I

T

L

I

I

G

G

D

E

G

N

A

A

I

M

V

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

A

D

G

N

A

A

R

I

V

V

V
x
I

G

G

N

N

P

P

A

G

R
|
R

binding coenzyme a: F72 (≠ L121), T80 (≠ A129), N81 (vs. gap), D82 (vs. gap), K83 (vs. gap), P85 (= P131), G99 (= G141), A100 (≠ G142), G117 (= G159), A118 (= A160), T123 (= T165), K124 (= K166), I133 (≠ V175), R138 (= R180)
binding magnesium ion: G58 (= G107), N76 (≠ G125)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
41% identity, 37% coverage: 116:184/185 of query aligns to 92:162/209 of 8j40A

query
sites
8j40A

H

Q

K

E

D

E

P

P

A

A

L

F

R

S

S

R

-

A

-

L

E

D

G

A

I

F

E

Q

I

R

A

A

R

G

P

D

V

T

A

V

I

I

G

G

S

N

D

D

V

V

W
|
W

I

I

G

G

G

S

G

E

T

A

I

M

I

I

L

M

G

P

G

G

V

I

T

K

I

I

G

G

D

D

G

G

A

A

I
x
V

V

I

G

G

A
x
S

G

R

A

S

V

L

V

V

T

T

K

K

D

D

V

V

P

E

A

P

G

Y

A

A

R

I

V

I

V

G

G

G

N

N

P

P

A

A

R

K

P

Q

I

I

N

K

R

K

binding chloramphenicol: W117 (= W139), V135 (≠ I157), S138 (≠ A160)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 90, 187, 190, 191, 202, 203, 206

8vr6A Crystal structure of the pcryo_0619 n-acetryltransferase from psychrobacter cryohalolentis k5 in the presence of coa-disulfide
34% identity, 52% coverage: 84:179/185 of query aligns to 45:165/177 of 8vr6A

query
sites
8vr6A

F

F

N

G

A

E

G

G

C

T

T

S

I

I

L

Y

D

D

S

S

-

S

-

I

-

V

-

L

G

G

S

E

V

V

T

K

I

V

G

G

D

K

R

D

T

T

M

W

L

I

G

G

P

P

-

N

-

T

-

I

-

L

-

D

-

G

-

S

-

G

-

L

-

I

-

G

-

S

-

N

-

C

-

S

-

I

-

S

-

A

G

G

V

V

Q

Q

I

I

Y
|
Y

C

T

A
x
H

E
x
D

H
x
T

H

V

K

R

D

-

P

K

A

S

L

L

R

S

S

G

E

G

G

K

I

A

E

D

I

I

A

D

R

K

-

A

P

S

V

T

A

R

I

I

G

G

S

S

D

D

V

C

W

Y

I

L

G

G

G

P

G

N

T

T

I

I

L
x
V

G

K

G

G

V

V

T

K

I

I

G

G

D

D

G

R

A

V

I

V

V

V

G

G

A

A

G

N

A

S

V
x
L

V

V

T
x
L

K

K

D

D

V

I

P

P

A

S

G

D

A

C

R

K

V

V

V

F

G

G

N
x
S

P
|
P

A

A

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: Y97 (= Y111), H99 (≠ A113), D100 (≠ E114), T101 (≠ H115), V133 (≠ L147), L149 (≠ V163), L151 (≠ T165), S163 (≠ N177), P164 (= P178)

8vr7B Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
34% identity, 52% coverage: 84:179/185 of query aligns to 46:166/177 of 8vr7B

query
sites
8vr7B

F

F

N

G

A

E

G

G

C

T

T

S

I

I

L

Y

D

D

S

S

-

S

-

I

-

V

-

L

G

G

S

E

V

V

T

K

I

V

G

G

D

K

R

D

T

T

M

W

L

I

G

G

P

P

-

N

-

T

-

I

-

L

-

D

-

G

-

S

-

G

-

L

-
x
I

-

I

-

G

-

S

-

N

-

C

-

S

-

I

-

S

-

A

G

G

V

V

Q

Q

I

I

Y
|
Y

C

T

A

H

E
x
D

H
x
T

H

V

K

R

D

-

P

K

A

S

L

L

R

S

S

G

E

G

G

K

I

A

E

D

I

I

A

D

R

K

-

A

P

S

V

T

A
x
R

I

I

G

G

S

S

D

D

V

C

W
x
Y

I

L

G

G

G
x
P

G

N

T

T

I

I

L

V

G

K

G

G

V

V

T

K

I

I

G

G

D

D

G

R

A

V

I

V

V

V

G
|
G

A
|
A

G

N

A

S

V
x
L

V

V

T
x
L

K

K

D

D

V

I

P

P

A

S

G

D

A

C

R
x
K

V

V

V
x
F

G

G

N

S

P
|
P

A

A

binding acetyl coenzyme *a: Y98 (= Y111), D101 (≠ E114), T102 (≠ H115), L150 (≠ V163), L152 (≠ T165), P165 (= P178)
binding coenzyme a: Y126 (≠ W139), P129 (≠ G142), G146 (= G159), A147 (= A160), K160 (≠ R173), F162 (≠ V175)
binding 3'-phosphate-adenosine-5'-diphosphate: I84 (vs. gap), R120 (≠ A133)

Sites not aligning to the query:

8vr7E Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
34% identity, 52% coverage: 84:179/185 of query aligns to 46:166/180 of 8vr7E

query
sites
8vr7E

F

F

N

G

A

E

G

G

C

T

T

S

I

I

L

Y

D

D

S

S

-

S

-

I

-

V

-

L

G

G

S

E

V

V

T

K

I

V

G

G

D

K

R

D

T

T

M
x
W

L

I

G

G

P

P

-

N

-

T

-

I

-

L

-

D

-

G

-

S

-

G

-

L

-

I

-

G

-

S

-

N

-

C

-

S

-

I

-
x
S

-

A

G

G

V

V

Q

Q

I

I

Y
|
Y

C

T

A

H

E
x
D

H
x
T

H

V

K

R

D

-

P

K

A

S

L

L

R

S

S

G

E

G

G

K

I

A

E

D

I

I

A

D

R

K

-

A

P

S

V

T

A

R

I

I

G

G

S

S

D

D

V

C

W
x
Y

I

L

G

G

G

P

G

N

T

T

I

I

L
x
V

G

K

G

G

V

V

T

K

I

I

G

G

D

D

G

R

A

V

I

V

V

V

G
|
G

A
|
A

G

N

A

S

V
x
L

V

V

T
x
L

K

K

D

D

V

I

P

P

A

S

G

D

A

C

R
x
K

V

V

V
x
F

G

G

N
x
S

P
|
P

A

A

binding acetyl coenzyme *a: W69 (≠ M103), S92 (vs. gap), Y98 (= Y111), D101 (≠ E114), T102 (≠ H115), Y126 (≠ W139), V134 (≠ L147), G146 (= G159), A147 (= A160), L150 (≠ V163), L152 (≠ T165), K160 (≠ R173), F162 (≠ V175), S164 (≠ N177), P165 (= P178)

Sites not aligning to the query:

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
34% identity, 59% coverage: 75:184/185 of query aligns to 29:166/205 of 1kk4A

query
sites
1kk4A

N

N

I

V

V

E

L

V

G

G

E

E

R

Y

V

S

Y

Y

F

Y

N

D

A

S

-

K

-

N

G

G

C

E

T

T

I

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-

Y

-

P

L

I

D

L

S

N

G

D

S

K

V

L

T

K

I

I

G

G

D

K

R

F

T

C

M

S

L
x
I

G
|
G

P

P

G

G

V

V

Q

T

I

I

Y

I

C

M

-

N

-

G

A

A

E

N

H

H

H

R

K

M

D

D

P

G

A

S

-

T

-

Y

-

P

-

F

-

N

L

L

R

F

S

G

E

N

G

G

I

W

E

E

I

K

A

H

R

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-
x
K

-

G

-

D

V

T

A

I

I

I

G

G

S

N

D

D

V

V

W
|
W

I

I

G
|
G

G
x
K

G

D

T

V

I

V

I

I

L

M

G

P

G

G

V

V

T

K

I

I

G

G

D

D

G

G

A

A

I

I

V

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
V

K
|
K

D

D

V

I

P

A

A

P

G

Y

A

M

R
x
L

V

A

V
x
G

G
|
G

N

N

P
|
P

A

A

R

N

P

E

I
|
I

N

K

R

Q

binding acetyl coenzyme *a: I69 (≠ L104), G70 (= G105), K111 (vs. gap), W121 (= W139), G123 (= G141), K124 (≠ G142), A141 (≠ G159), A142 (= A160), V147 (≠ T165), K148 (= K166), L155 (≠ R173), G157 (≠ V175), G158 (= G176), P160 (= P178), I164 (= I182)

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
34% identity, 59% coverage: 75:184/185 of query aligns to 29:166/204 of 1mrlA

query
sites
1mrlA

N

N

I

V

V

E

L

V

G

G

E

E

R

Y

V

S

Y
|
Y

F

Y

N
x
D

A

S

-

K

-

N

G

G

C

E

T

T

I

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-
x
Y

-

P

L
x
I

D

L

S

N

G

D

S

K

V

L

T

K

I

I

G

G

D

K

R

F

T

C

M

S

L

I

G

G

P

P

G

G

V

V

Q

T

I

I

Y

I

C

M

-

N

-

G

A

A

E
x
N

H
|
H

H

R

K

M

D

D

P

G

A

S

-

T

-

Y

-

P

-

F

-

N

L
|
L

R

F

S

G

E

N

G

G

I

W

E

E

I

K

A

H

R
x
M

P

P

-

K

-

L

-

D

-

Q

-
x
L

-

P

-

I

-

K

-

G

-

D

V

T

A

I

I

I

G

G

S

N

D

D

V

V

W

W

I

I

G

G

G

K

G

D

T

V

I

V

I

I

L

M

G

P

G

G

V

V

T

K

I

I

G

G

D

D

G

G

A

A

I

I

V

V

G

A

A

A

G

N

A

S

V

V

T

V

K

K

D

D

V

I

P

A

A

P

G

Y

A

M

R

L

V

A

V

G

G

G

N

N

P

P

A

A

R

N

P

E

I

I

N

K

R

Q

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: Y37 (= Y83), D39 (≠ N85), Y54 (vs. gap), I56 (≠ L91), N81 (≠ E114), H82 (= H115), L93 (= L121), M102 (≠ R130), L108 (vs. gap)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
34% identity, 59% coverage: 75:184/185 of query aligns to 29:166/206 of 1khrA

query
sites
1khrA

N

N

I

V

V

E

L

V

G

G

E

E

R

Y

V

S

Y
|
Y

F

Y

N
x
D

A

S

-

K

-

N

G

G

C

E

T

T

I

F

-

D

-

K

-

Q

-

I

-

L

-

Y

-

H

-
x
Y

-

P

L

I

D

L

S

N

G

D

S

K

V

L

T

K

I

I

G

G

D

K

R

F

T

C

M

S

L

I

G

G

P

P

G

G

V

V

Q

T

I

I

Y

I

C

M

-

N

-

G

A
|
A

E
x
N

H
|
H

H

R

K
x
M

D

D

P

G

A

S

-

T

-

Y

-

P

-

F

-

N

L
|
L

R

F

S

G

E

N

G

G

I

W

E

E

I

K

A

H

R

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-

K

-

G

-

D

V

T

A

I

I

I

G

G

S

N

D

D

V

V

W
|
W

I

I

G

G

G

K

G

D

T

V

I

V

I

I

L

M

G

P

G

G

V

V

T

K

I

I

G

G

D

D

G

G

A

A

I

I

V

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
V

K

K

D

D

V

I

P

A

A

P

G

Y

A

M

R
x
L

V

A

V
x
G

G

G

N

N

P
|
P

A

A

R

N

P

E

I
|
I

N

K

R

Q

binding coenzyme a: H82 (= H115), M84 (≠ K117), W121 (= W139), A141 (≠ G159), A142 (= A160), V147 (≠ T165), L155 (≠ R173), G157 (≠ V175), P160 (= P178), I164 (= I182)
binding virginiamycin m1: Y37 (= Y83), D39 (≠ N85), Y54 (vs. gap), A80 (= A113), N81 (≠ E114), H82 (= H115), L93 (= L121)

Query Sequence

>WP_011423631.1 NCBI__GCF_000092045.1:WP_011423631.1
MPASEREKMAAGEWYCCLDHELDILRRQARVAVHAHNSLPPDERGAVAPALRRLFAEAAP
EVFIEAPFHCSYGINIVLGERVYFNAGCTILDSGSVTIGDRTMLGPGVQIYCAEHHKDPA
LRSEGIEIARPVAIGSDVWIGGGTIILGGVTIGDGAIVGAGAVVTKDVPAGARVVGNPAR
PINRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory