SitesBLAST
Comparing WP_011423940.1 NCBI__GCF_000092045.1:WP_011423940.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
41% identity, 97% coverage: 5:342/350 of query aligns to 6:346/362 of 3bptA
- active site: G67 (= G66), P84 (≠ G83), R88 (= R87), G115 (= G114), G118 (= G117), E138 (= E137), D146 (= D145)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G65), G67 (= G66), I69 (= I68), E90 (= E89), G114 (= G113), G115 (= G114), E138 (= E137), D146 (= D145), V147 (= V146)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A24), L26 (= L25), A28 (≠ S27), G66 (= G65), G67 (= G66), I69 (= I68), P137 (= P136), I141 (= I140), L319 (≠ V315)
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
41% identity, 99% coverage: 3:350/350 of query aligns to 1:337/340 of 4hdtA
- active site: G64 (= G66), I69 (≠ L71), W84 (= W86), Y88 (≠ F90), G112 (= G114), G115 (= G117), E135 (= E137), P142 (= P144), D143 (= D145), R283 (≠ G292)
- binding zinc ion: H28 (≠ L30), E42 (≠ A44), E57 (= E59), E79 (≠ L81), H93 (≠ M95), H185 (≠ S187)
Sites not aligning to the query:
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 98% coverage: 3:345/350 of query aligns to 8:357/378 of Q9LKJ1
- G70 (= G66) mutation to S: Loss of activity.
- E142 (= E137) mutation to A: Loss of activity.
- D150 (= D145) mutation to G: Reduced activity.
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
35% identity, 50% coverage: 6:180/350 of query aligns to 6:178/260 of 2hw5C
- active site: A68 (≠ G66), M73 (≠ L71), S83 (≠ A82), L87 (≠ W86), G111 (= G114), E114 (≠ G117), P133 (= P136), E134 (= E137), T139 (≠ Y142), P141 (= P144), G142 (≠ D145)
- binding crotonyl coenzyme a: K26 (≠ R23), A27 (= A24), L28 (= L25), A30 (≠ S27), K62 (≠ R60), I70 (= I68), F109 (≠ M112)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
34% identity, 50% coverage: 6:180/350 of query aligns to 6:178/260 of 1dubA
- active site: A68 (≠ G66), M73 (≠ L71), S83 (≠ A82), L87 (= L92), G111 (= G114), E114 (≠ G117), P133 (= P136), E134 (= E137), T139 (≠ Y142), P141 (= P144), G142 (≠ D145)
- binding acetoacetyl-coenzyme a: K26 (≠ R23), A27 (= A24), L28 (= L25), A30 (≠ S27), A66 (= A64), A68 (≠ G66), D69 (= D67), I70 (= I68), Y107 (≠ I110), G110 (= G113), G111 (= G114), E114 (≠ G117), P133 (= P136), E134 (= E137), L137 (≠ I140), G142 (≠ D145)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
34% identity, 50% coverage: 6:180/350 of query aligns to 36:208/290 of P14604
- E144 (≠ G117) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E137) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
34% identity, 50% coverage: 6:180/350 of query aligns to 4:176/258 of 1ey3A
- active site: A66 (≠ G66), M71 (≠ L71), S81 (≠ A82), L85 (= L92), G109 (= G114), E112 (≠ G117), P131 (= P136), E132 (= E137), T137 (≠ Y142), P139 (= P144), G140 (≠ D145)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ R23), L26 (= L25), A28 (≠ S27), A64 (= A64), G65 (= G65), A66 (≠ G66), D67 (= D67), I68 (= I68), L85 (= L92), W88 (vs. gap), G109 (= G114), P131 (= P136), L135 (≠ I140), G140 (≠ D145)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
33% identity, 50% coverage: 6:180/350 of query aligns to 5:172/254 of 2dubA
- active site: A67 (≠ G66), M72 (≠ L71), S82 (≠ F90), G105 (= G114), E108 (≠ G117), P127 (= P136), E128 (= E137), T133 (≠ Y142), P135 (= P144), G136 (≠ D145)
- binding octanoyl-coenzyme a: K25 (≠ R23), A26 (= A24), L27 (= L25), A29 (≠ S27), A65 (= A64), A67 (≠ G66), D68 (= D67), I69 (= I68), K70 (≠ R69), G105 (= G114), E108 (≠ G117), P127 (= P136), E128 (= E137), G136 (≠ D145), A137 (≠ V146)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
34% identity, 50% coverage: 6:180/350 of query aligns to 6:176/258 of 1mj3A
- active site: A68 (≠ G66), M73 (≠ L71), S83 (≠ F90), L85 (= L92), G109 (= G114), E112 (≠ G117), P131 (= P136), E132 (= E137), T137 (≠ Y142), P139 (= P144), G140 (≠ D145)
- binding hexanoyl-coenzyme a: K26 (≠ R23), A27 (= A24), L28 (= L25), A30 (≠ S27), A66 (= A64), G67 (= G65), A68 (≠ G66), D69 (= D67), I70 (= I68), G109 (= G114), P131 (= P136), E132 (= E137), L135 (≠ I140), G140 (≠ D145)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
29% identity, 55% coverage: 1:194/350 of query aligns to 1:198/259 of 5zaiC
- active site: A65 (≠ G66), F70 (≠ L71), S82 (≠ W86), R86 (≠ F90), G110 (= G114), E113 (≠ G117), P132 (= P136), E133 (= E137), I138 (≠ Y142), P140 (= P144), G141 (≠ D145)
- binding coenzyme a: K24 (≠ A24), L25 (= L25), A63 (= A64), G64 (= G65), A65 (≠ G66), D66 (= D67), I67 (= I68), P132 (= P136), R166 (≠ D169)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 49% coverage: 6:175/350 of query aligns to 5:165/250 of 3q0gD
- active site: A64 (≠ G66), M69 (≠ L71), T75 (≠ A77), F79 (≠ L81), G103 (= G114), E106 (≠ G117), P125 (= P136), E126 (= E137), V131 (≠ Y142), P133 (= P144), G134 (≠ D145)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
30% identity, 50% coverage: 1:175/350 of query aligns to 1:170/255 of 3q0jC
- active site: A65 (≠ G66), M70 (≠ L71), T80 (≠ W86), F84 (= F90), G108 (= G114), E111 (≠ G117), P130 (= P136), E131 (= E137), V136 (≠ Y142), P138 (= P144), G139 (≠ D145)
- binding acetoacetyl-coenzyme a: Q23 (≠ R23), A24 (= A24), L25 (= L25), A27 (≠ S27), A63 (= A64), G64 (= G65), A65 (≠ G66), D66 (= D67), I67 (= I68), K68 (≠ R69), M70 (≠ L71), F84 (= F90), G107 (= G113), G108 (= G114), E111 (≠ G117), P130 (= P136), E131 (= E137), P138 (= P144), G139 (≠ D145), M140 (≠ V146)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 50% coverage: 1:175/350 of query aligns to 1:170/255 of 3q0gC
- active site: A65 (≠ G66), M70 (≠ L71), T80 (≠ W86), F84 (= F90), G108 (= G114), E111 (≠ G117), P130 (= P136), E131 (= E137), V136 (≠ Y142), P138 (= P144), G139 (≠ D145)
- binding coenzyme a: L25 (= L25), A63 (= A64), I67 (= I68), K68 (≠ R69), Y104 (≠ I110), P130 (= P136), E131 (= E137), L134 (≠ I140)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
29% identity, 48% coverage: 6:174/350 of query aligns to 24:194/1413 of 6z1pBI
- active site: T85 (≠ G66), S134 (≠ G114), E157 (= E137), D165 (= D145)
- binding : Y41 (≠ R23), K42 (≠ A24), Q43 (≠ L25), T45 (≠ S27), D47 (≠ T29), H49 (≠ A31), K83 (≠ A64), T85 (≠ G66), D86 (= D67), F87 (≠ I68), K88 (≠ R69), K92 (≠ E73), L130 (≠ I110), K152 (≠ R132)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
30% identity, 49% coverage: 6:175/350 of query aligns to 5:169/256 of 3h81A
- active site: A64 (≠ G66), M69 (≠ L71), T79 (≠ W86), F83 (= F90), G107 (= G114), E110 (≠ G117), P129 (= P136), E130 (= E137), V135 (≠ Y142), P137 (= P144), G138 (≠ D145)
Sites not aligning to the query:
7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
32% identity, 45% coverage: 4:159/350 of query aligns to 1:150/247 of 7borA
- active site: N63 (≠ G66), F68 (≠ A70), D77 (= D79), G81 (= G83), I105 (≠ G114), T108 (≠ G117), F128 (≠ E137), L133 (≠ Y142), P135 (= P144), E136 (≠ D145)
- binding coenzyme a: D21 (≠ R23), K22 (≠ A24), A25 (≠ S27), S61 (≠ A64), I65 (= I68), V103 (≠ M112), F128 (≠ E137), L131 (≠ I140)
Sites not aligning to the query:
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
31% identity, 51% coverage: 16:194/350 of query aligns to 13:182/723 of Q08426
- V40 (≠ A44) to G: in dbSNP:rs1062551
- I41 (≠ R45) to R: in dbSNP:rs1062552
- T75 (≠ A82) to I: in dbSNP:rs1062553
- K165 (≠ Y177) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ L183) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
- 274 A → T: in dbSNP:rs2302819
- 325 A → G: in dbSNP:rs1062555
- 346 modified: N6-acetyllysine; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- 584 modified: N6-acetyllysine; alternate; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- 598 K → T: in dbSNP:rs1042437
- 606 T → P: in dbSNP:rs1042438
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
28% identity, 49% coverage: 5:175/350 of query aligns to 9:179/266 of O53561
- K135 (≠ R132) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 132:139, 13% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ G139) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
29% identity, 44% coverage: 6:159/350 of query aligns to 2:141/245 of 6slaAAA
- active site: Q61 (≠ G66), L68 (≠ W86), N72 (≠ F90), A96 (≠ G114), S99 (≠ G117), A118 (≠ P136), F119 (≠ E137), L124 (≠ Y142), P126 (= P144), N127 (≠ D145)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L25), A59 (= A64), Q61 (≠ G66), D62 (= D67), L63 (≠ I68), L68 (≠ W86), Y71 (≠ E89), A94 (≠ M112), G95 (= G113), A96 (≠ G114), F119 (≠ E137), I122 (= I140), L124 (≠ Y142), N127 (≠ D145)
Sites not aligning to the query:
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 44% coverage: 6:159/350 of query aligns to 5:153/257 of 6slbAAA