SitesBLAST

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
44% identity, 79% coverage: 24:156/168 of query aligns to 32:157/191 of G3XD01

query
sites
G3XD01

G

G

C

A

T

R

I

I

G

G

A

A

G

G

T

V

R

S

I

L

G

G

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Q

F

N

V

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E

F

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K

N

N

K

V

V

V

I

G

G

R

D

D

R

C

C

K

K

I

I

S

Q

S

N

H

N

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F

S

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V

C

Y

E

D

G

N

V

V

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T

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L

E

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D

E

G

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V

F

F

I

C

G

G

H

P

G

S

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M

M

V

F

F

T

T

N

N

D

V

T

Y

Y

N

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P

R

R

A

S

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N

-

P

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D

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G

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D

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N

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T

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L

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V

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K

R

K

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G

A

A

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G

G

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A

N

N

A

C

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T

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C

G

G

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V

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T

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I

G

G

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Y

A

A

Q

F

V

V

G

G

A

A

G

G

A

A

V

V

V

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T

N

K
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K

D

N

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P

D

S

G

Y

A

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M

A

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A

R

R

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G

R

W

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N

S

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E

K136 (= K135) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
40% identity, 76% coverage: 11:137/168 of query aligns to 1:131/141 of 7s45A

query
sites
7s45A

G

G

S

H

V

M

I

I

H

H

H

-

R

-

D

K

L

L

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A

N

D

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G

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C

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N

I

I

G

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A

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R

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F

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A

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G

G

R

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D

N

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C

K

N

I

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S

S

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H

S

C

F
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F

L

I

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x
E

-

N

-

D

-

V

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K

-

I

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G

-

N

-

V

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T

-

I

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K

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-

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x
W

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D

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G

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L

I

E

E

D

D

G

D

V

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F

F

I

I

G

G

H

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G

N

V

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M

T

F

F

T
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T

N
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N

D
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D

T
x
K

Y
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Y

P
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P

R

R

A

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I

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N

Q

P

-

D

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G

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S

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L

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K

R

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H

G

A

A

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S

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G

G

S

A

N

N

A

A

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L

P

P

G

G

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I

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I

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G

G

E

E

A

N

A

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M

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A

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G

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T

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V

binding acetyl coenzyme *a: W64 (≠ C58), T82 (= T76), N83 (= N77), D84 (= D78), K85 (≠ T79), Y86 (= Y80), P87 (= P81), L110 (= L116), T128 (= T134), K129 (= K135)
binding thymidine-5'-diphosphate: F44 (= F56), E46 (vs. gap)

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
41% identity, 76% coverage: 11:137/168 of query aligns to 1:135/145 of 7s42A

query
sites
7s42A

G

G

S

H

V

M

I

I

H

H

H

-

R

-

D

K

L

L

V

A

N

D

L

V

Y

Q

G

S

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K

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N

I

I

G

G

A

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G

G

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R

I

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F

F

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A

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I

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G

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E

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N

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C

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N

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S

S

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S

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F
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F

L

I

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x
E

-

N

-

D

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K

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I

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G

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N

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x
W

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L

I

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E

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D

G

D

V

V

F

F

I

I

G

G

H

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G

N

V

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M

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F

F

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x
C

N
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N

D
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D

T
x
K

Y
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Y

P
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P

R

R

A

-

I

-

N

-

P

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D

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G

-

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L

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x
Y

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W

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I

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L

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V

I

K

K

R

K

H

G

A

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
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L

P

P

G

G

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I

I

T

I

I

G

G

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E

A

N

A

A

Q

M

V

I

G

G

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A

G

G

A

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T

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D

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V

binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (= F56), E46 (vs. gap), W64 (≠ C58), N83 (= N77), D84 (= D78), Y92 (≠ T92)
binding coenzyme a: C82 (≠ T76), D84 (= D78), K85 (≠ T79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (= K135)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
41% identity, 76% coverage: 11:137/168 of query aligns to 1:135/145 of 7s41A

query
sites
7s41A

G

G

S

H

V

M

I

I

H

H

H

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R

-

D

K

L

L

V

A

N

D

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Y

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G

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C

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N

I

I

G

G

A

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G

G

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T

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R

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I

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F

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N

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A

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G

G

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D

N

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N

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H

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F

L

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x
E

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N

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K

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N

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x
W

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G

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L

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G

D

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F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

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x
C

N
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N

D
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D

T
x
K

Y
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Y

P
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P

R

R

A

-

I

-

N

-

P

-

D

-

G

-

S

S

L

K

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Q

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x
Y

E

P

T

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D

E

W

-

I

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L

F

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K

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T

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I

V

I

K

K

R

K

H

G

A

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
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L

P

P

G

G

V

I

I

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I

I

G

G

E

E

A

N

A

A

Q

M

V

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G

G

A

A

G

G

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T

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K

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D

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V

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (= F56), E46 (vs. gap), W64 (≠ C58), N83 (= N77), D84 (= D78), Y92 (≠ T92)
binding coenzyme a: C82 (≠ T76), D84 (= D78), K85 (≠ T79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (= K135)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
41% identity, 76% coverage: 11:137/168 of query aligns to 1:135/145 of 7s3wA

query
sites
7s3wA

G

G

S

H

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I

I

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H

H

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R

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x
C

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N

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D

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Y
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Y

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P

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R

A

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I

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N

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K

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G

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S

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G

S

A

N

N

A

A

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L

P

P

G

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D

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V

binding oxidized coenzyme a: C82 (≠ T76), D84 (= D78), K85 (≠ T79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (= K135)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F56), E46 (vs. gap), D84 (= D78), Y92 (≠ T92)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
41% identity, 76% coverage: 11:137/168 of query aligns to 1:135/145 of 7s3uA

query
sites
7s3uA

G

G

S

H

V

M

I

I

H

H

H

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R

-

D

K

L

L

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A

N

D

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G

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N

I

I

G

G

A

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R

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F

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G

G

R

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D

N

C

C

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N

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H

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F
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F

L

I

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x
E

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N

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D

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K

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I

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N

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I

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E

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D

G

D

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V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N
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N

D
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D

T
x
K

Y
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Y

P
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P

R

R

A

-

I

-

N

-

P

-

D

-

G

-

S

S

L

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Q

T
x
Y

E

P

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K

D

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W

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I

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L

F

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K

T

T

L

I

V

I

K

K

R

K

H

G

A

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
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L

P

P

G

G

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I

T

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I

G

G

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E

A

N

A

A

Q

M

V

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G

G

A

A

G

G

A

A

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T

K
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K

D

D

V

V

binding coenzyme a: C82 (≠ T76), N83 (= N77), D84 (= D78), K85 (≠ T79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (= K135)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F56), E46 (vs. gap), D84 (= D78), Y92 (≠ T92)

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
41% identity, 77% coverage: 24:152/168 of query aligns to 32:153/191 of 3mqhA

query
sites
3mqhA

G

G

C

A

T

E

I

I

G

G

A

E

G

G

T

C

R

S

I

L

G

G

T

Q

F

N

V

V

E
x
F

I

V

Q

G

K

N

N

R

V

V

V

R

V

I

G

G

R

N

D

R

C

V

K

K

I

I

S
x
Q

S
x
N

H

N

S

V

F

S

L

V

C
x
Y

E

D

G

N

V

V

T

F

L

L

E

E

D

D

G

D

V

V

F
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F

I

C

G

G

H

P

G

S

V

M

M

V

F

F

T
|
T

N
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N

D

-

T

-

Y

-

P

-

R

-

A
x
V

I
x
Y

N

N

P
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P

D

R

G

A

S

A

L

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Q

E

T
x
R

E

K

T

S

D

E

W
x
Y

I

R

L

-

I

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P

D

T

T

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I

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V

K

R

R

Q

H

G

A

A

S

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I

L

G
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G

S
x
A

N

N

A

C

T

T

I

V

L

V

P

C

G

G

V

A

I

T

I

I

G

G

E

R

A

Y

A

A

Q

F

V

V

G

G

A
|
A

G

G

A

A

V

V

T
x
N

K

K

D

D

V

V

P

P

D

D

G

F

A

A

I

L

V

V

A
x
V

G

G

V

V

P
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P

A

A

R
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R

V

Q

I

I

G

G

binding coenzyme a: F76 (= F68), T84 (= T76), N85 (= N77), V86 (≠ A83), Y87 (≠ I84), P89 (= P86), G111 (= G110), A112 (≠ S111), A130 (= A129), N135 (≠ T134), V145 (≠ A144), P148 (= P147), R150 (= R149)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: F46 (≠ E38), Q60 (≠ S52), N61 (≠ S53), Y66 (≠ C58), F76 (= F68), N85 (= N77), V86 (≠ A83), R95 (≠ T92), Y99 (≠ W96)

Sites not aligning to the query:

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
41% identity, 77% coverage: 24:152/168 of query aligns to 33:154/192 of 3mqgC

query
sites
3mqgC

G

G

C

A

T

E

I

I

G

G

A

E

G

G

T

C

R
x
S

I

L

G

G

T

Q

F

N

V

V

E
x
F

I

V

Q

G

K

N

N

R

V

V

V

R

V

I

G

G

R

N

D

R

C

V

K

K

I

I

S

Q

S
x
N

H

N

S

V

F

S

L

V

C
x
Y

E

D

G

N

V

V

T

F

L

L

E

E

D

D

G

D

V

V

F
|
F

I

C

G

G

H
x
P

G

S

V

M

M

V

F

F

T
|
T

N
|
N

D

-

T

-

Y

-

P

-

R

-

A
x
V

I
x
Y

N

N

P
|
P

D

R

G

A

S

A

L

I

Q

E

T

R

E

K

T

S

D

E

W

Y

I

R

L

-

I

-

P

D

T

T

L

I

V

V

K

R

R

Q

H

G

A

A

S

T

I

L

G
|
G

S
x
A

N

N

A

C

T

T

I

V

L

V

P

C

G

G

V

A

I

T

I

I

G

G

E

R

A

Y

A

A

Q
x
F

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T
x
N

K

K

D

D

V

V

P

P

D

D

G

F

A

A

I

L

V

V

A
x
V

G

G

V

V

P
|
P

A

A

R
|
R

V

Q

I

I

G

G

binding acetyl coenzyme *a: Y67 (≠ C58), F77 (= F68), P80 (≠ H71), T85 (= T76), N86 (= N77), V87 (≠ A83), Y88 (≠ I84), P90 (= P86), G112 (= G110), A113 (≠ S111), F128 (≠ Q126), G130 (= G128), A131 (= A129), N136 (≠ T134), V146 (≠ A144), P149 (= P147), R151 (= R149)
binding uridine-5'-monophosphate: F47 (≠ E38)
binding uridine-5'-diphosphate: S41 (≠ R32), N62 (≠ S53)

Sites not aligning to the query:

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
39% identity, 74% coverage: 27:151/168 of query aligns to 107:219/260 of 3fsbA

query
sites
3fsbA

I

I

G

G

A

N

G

N

T

V

R

K

I

I

G
|
G

T

T

F

L

V

S

E

D

I

I

Q

Q

K

H

N

H

V

V

V

Y

V

I

G

G

R

N

D

Y

C

V

K

N

I

I

S
x
H

S

S

H

N

S

V

F

F

L

V

C

G

E

E

G

K

V

S

T

I

L

I

E

K

D

D

G

F

V

V

F

W

I

L

G
x
F

H

P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

T

P

Y

T

P

P

R

-

A

-

I

-

N

-

P

P

D

S

G

N

S

E

L

L

Q

L

T

G

E

V

T

T

D

-

W

-

I

-

L

-

I

-

P

-

T

-

L

-

V

I

K

E

R

L

H

F

A

A

S

V

I

I

G
x
A

S
x
A

N

R

A

S

T

V

I

V

L

L

P

P

G

G

V

I

I

H

I

I

G

N

E

E

A

D

A

A

Q

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

D

K

G

E

A

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

R

R

V

E

I

I

binding coenzyme a: F150 (≠ G70), A178 (≠ G110), A179 (≠ S111), G196 (= G128), A197 (= A129), V212 (≠ A144)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G34), H132 (≠ S52), F150 (≠ G70)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
38% identity, 76% coverage: 24:151/168 of query aligns to 104:219/259 of 3fscA

query
sites
3fscA

G

N

C

T

T

K

I

I

G

G

A

N

G

N

T

V

R
x
K

I

I

G
|
G

T
|
T

F

L

V

S

E

D

I

I

Q

Q

K

H

N

H

V

V

V

Y

V

I

G

G

R

N

D

Y

C

V

K

N

I

I

S
x
H

S
|
S

H

N

S

V

F

F

L

V

C

G

E

E

G

K

V

S

T

I

L

I

E

K

D

D

G

F

V

V

F

W

I

L

G
x
F

H

P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

T

P

Y

T

P

P

R

-

A

-

I

-

N

-

P

P

D

S

G

N

S

E

L

L

Q

L

T

G

E

V

T

T

D

-

W

-

I

-

L

-

I

-

P

-

T

-

L

-

V

I

K

E

R

L

H

F

A

A

S

V

I

I

G
x
A

S
x
A

N

R

A

S

T

V

I

V

L

L

P

P

G

G

V

I

I

H

I

I

G

N

E

E

A

D

A

A

Q

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

D

K

G

E

A

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

R

R

V

E

I

I

binding coenzyme a: F150 (≠ G70), A178 (≠ G110), A179 (≠ S111), G196 (= G128), A197 (= A129), V212 (≠ A144)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ R32), G114 (= G34), T115 (= T35), H132 (≠ S52), S133 (= S53)

Sites not aligning to the query:

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
38% identity, 76% coverage: 24:151/168 of query aligns to 104:219/259 of 3fs8A

query
sites
3fs8A

G

N

C

T

T

K

I

I

G

G

A

N

G

N

T

V

R

K

I

I

G

G

T

T

F

L

V

S

E

D

I

I

Q

Q

K

H

N

H

V

V

V

Y

V

I

G

G

R

N

D

Y

C

V

K

N

I

I

S

H

S

S

H

N

S

V

F

F

L

V

C

G

E

E

G

K

V

S

T

I

L

I

E

K

D

D

G

F

V

V

F

W

I

L

G
x
F

H
x
P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

T

P

Y

T

P

P

R

-

A

-

I

-

N

-

P

P

D

S

G

N

S

E

L

L

Q

L

T

G

E

V

T

T

D

-

W

-

I

-

L

-

I

-

P

-

T

-

L

-

V

I

K

E

R

L

H

F

A

A

S

V

I

I

G
x
A

S
x
A

N

R

A

S

T

V

I

V

L

L

P

P

G

G

V

I

I

H

I

I

G

N

E

E

A

D

A

A

Q

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

D

K

G

E

A

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

R

R

V

E

I

I

binding acetyl coenzyme *a: F150 (≠ G70), P151 (≠ H71), A178 (≠ G110), A179 (≠ S111), G196 (= G128), A197 (= A129), V212 (≠ A144)

Sites not aligning to the query:

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
32% identity, 70% coverage: 42:158/168 of query aligns to 74:189/190 of 5u2kA

query
sites
5u2kA

N

N

V

V

V

K

V

L

G

G

R

K

D

N

C

V

K

Y

I

V

S

N

S

T

H

N

S

C

F

Y

L

F

C
x
M

E

D

G

G

-

G

-

Q

V

I

T

T

L

I

E

G

D

D

G

N

V

V

F

F

I

I

G

G

H

P

G

N

V

C

M

G

F

F

T
x
Y

N

T

D
x
A

T

T

Y
x
H

P

P

R

L

A

N

I

F

N

H

P

-

D

-

G

-

S

-

L

H

Q

R

T

N

E

E

T

G

D

F

W

E

I

K

L

A

I

G

P

P

T

I

L

H

V

I

K

G

R

S

H

N

A

T

S

W

I

F

G

G

S

G

N

H

A

V

T

A

I

V

L
|
L

P

P

G

G

V

V

I

T

I

I

G

G

E

E

A

G

A

S

Q

V

V

I

G

G

A

A

G

G

A

S

V

V

T
|
T

K
|
K

D

D

V

I

P

P

D

P

G

H

A

S

I

L

V

A

A

V

G

G

V

N

P

P

A

C

R

K

V

V

I

V

G

R

R

K

V

I

-

D

N

N

D

D

G

L

P

P

binding coenzyme a: M90 (≠ C58), Y110 (≠ T76), A112 (≠ D78), H114 (≠ Y80), L146 (= L116), T164 (= T134), K165 (= K135)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
36% identity, 57% coverage: 61:156/168 of query aligns to 95:186/201 of 1krvA

query
sites
1krvA

V

V

T

T

L

I

E

G

D

D

G

N

V

V

F

L

I

I

G
x
A

H

P

G

N

V

V

M

T

F

L

T
x
S

N

V

D
x
T

T

G

Y

H

P

P

R

V

A

-

I

-

N

-

P

-

D

H

G

H

S

E

L

L

Q

R

T

K

E

N

T

G

D

E

W
x
M

I

Y

L

S

I

F

P

P

T

I

L

T

V

I

K

G

R

N

H

N

A

V

S
x
W

I

I

G
|
G

S
|
S

N

H

A

V

T

V

I

I

L
x
N

P

P

G

G

V

V

I

T

I

I

G

G

E

D

A

N

A

S

Q

V

V

I

G

G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

D

P

G

N

A

V

I

V

V

A

A

A

G

G

V

V

P
|
P

A

C

R
|
R

V

V

I

I

G
x
R

R

E

V

I

N

N

D

D

binding 4-nitrophenyl beta-D-galactopyranoside: M126 (≠ W96)
binding coenzyme a: A104 (≠ G70), S110 (≠ T76), T112 (≠ D78), W138 (≠ S108), G140 (= G110), S141 (= S111), N146 (≠ L116), A159 (= A129), P177 (= P147), R179 (= R149), R182 (≠ G152)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70, 82, 84, 90, 92

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
36% identity, 57% coverage: 61:156/168 of query aligns to 95:186/201 of 1kruA

query
sites
1kruA

V

V

T

T

L

I

E

G

D

D

G

N

V

V

F
x
L

I

I

G

A

H

P

G

N

V

V

M

T

F

L

T

S

N

V

D

T

T

G

Y
x
H

P

P

R

V

A

-

I

-

N

-

P

-

D

H

G

H

S

E

L

L

Q

R

T

K

E

N

T

G

D

E

W
x
M

I

Y

L

S

I

F

P

P

T

I

L

T

V

I

K

G

R

N

H

N

A

V

S
x
W

I

I

G
|
G

S
|
S

N

H

A

V

T

V

I

I

L

N

P

P

G

G

V

V

I

T

I

I

G

G

E

D

A

N

A

S

Q

V

V

I

G
|
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

D

P

G

N

A

V

I

V

V

A

A

A

G

G

V

V

P
|
P

A

C

R

R

V

V

I

I

G
x
R

R

E

V

I

N

N

D

D

binding coenzyme a: H114 (≠ Y80), W138 (≠ S108), G140 (= G110), S141 (= S111), G158 (= G128), A159 (= A129), P177 (= P147), R182 (≠ G152)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: L102 (≠ F68), H114 (≠ Y80), M126 (≠ W96)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70, 82, 82, 84, 90, 92

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 57% coverage: 61:156/168 of query aligns to 96:187/203 of P07464

query
sites
P07464

V

V

T

T

L

I

E

G

D

D

G

N

V

V

F

L

I

I

G

A

H

P

G

N

V

V

M

T

F

L

T

S

N

V

D

T

T

G

Y
x
H

P

P

R

V

A

-

I

-

N

-

P

-

D

H

G

H

S

E

L

L

Q

R

T

K

E

N

T

G

D

E

W

M

I

Y

L

S

I

F

P

P

T

I

L

T

V

I

K

G

R

N

H

N

A

V

S

W

I

I

G

G

S
|
S

N

H

A

V

T

V

I

I

L

N

P

P

G

G

V

V

I

T

I

I

G

G

E

D

A

N

A

S

Q

V

V

I

G

G

A
|
A

G

G

A

S

V

I

V

V

T
|
T

K
|
K

D

D

V

I

P

P

D

P

G

N

A

V

I

V

V

A

A

A

G

G

V

V

P

P

A

C

R
|
R

V

V

I

I

G
x
R

R

E

V

I

N

N

D

D

H115 (≠ Y80) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S111) binding in other chain
A160 (= A129) binding in other chain
TK 165:166 (= TK 134:135) binding acetyl-CoA
R180 (= R149) binding acetyl-CoA
R183 (≠ G152) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate
85 binding in other chain; binding in other chain
93 binding substrate

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
36% identity, 57% coverage: 61:156/168 of query aligns to 95:186/200 of 1krrA

query
sites
1krrA

V

V

T

T

L

I

E

G

D

D

G

N

V

V

F

L

I
|
I

G
x
A

H
x
P

G

N

V

V

M

T

F

L

T

S

N

V

D
x
T

T

G

Y
x
H

P

P

R

V

A

-

I

-

N

-

P

-

D

H

G

H

S

E

L

L

Q

R

T

K

E

N

T

G

D

E

W

M

I

Y

L

S

I

F

P

P

T

I

L

T

V

I

K

G

R

N

H

N

A

V

S

W

I

I

G
|
G

S
|
S

N

H

A

V

T

V

I

I

L
x
N

P

P

G

G

V

V

I

T

I

I

G

G

E

D

A

N

A

S

Q

V

V

I

G
|
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

D

P

G

N

A

V

I

V

V

A

A
|
A

G

G

V

V

P
|
P

A

C

R

R

V

V

I

I

G
x
R

R

E

V

I

N

N

D

D

binding acetyl coenzyme *a: I103 (= I69), A104 (≠ G70), P105 (≠ H71), T112 (≠ D78), H114 (≠ Y80), G140 (= G110), S141 (= S111), N146 (≠ L116), G158 (= G128), A159 (= A129), A174 (= A144), P177 (= P147), R182 (≠ G152)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

7l82AAA Putative acetyl transferase protein (see paper)
35% identity, 75% coverage: 26:151/168 of query aligns to 108:216/216 of 7l82AAA

query
sites
7l82AAA

T

S

I

I

G

D

A

A

G

G

T

A

R

A

I

L

G

S

T

P

F

F

V

V

E

T

I

I

Q

A

K

A

N

N

V

V

V

T

V

I

G

G

R

K

D

C

C

F

K

H

I

A

S

N

S

L

H

Y

S

S

F

Y

L

V

C

-

E

-

G

-

V

-

T

-

L

-

E

E

D

H

G

D

V

C

F

I

I

I

G

G

H

D

G

Y

V

V

M

T

F

F

T

A

N

-

D

-

T

-

Y

-

P

P

R

R

A

-

I

V

N

S

P

C

D

N

G

G

S

N

L

I

Q

H

T

-

E

-

T

-

D

-

W

-

I

-

L

-

I

-

P

-

T

-

L

-

V

I

K

H

R

D

H

H

A

A

S
x
Y

I

I

G

G

S
x
T

N

G

A

A

T

V

I

I

L

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

I

I

G

G

E

K

A

G

A

A

Q

I

V

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

G

G

A

A

I

V

V

V

A
x
I

G

G

V

N

P

P

A

A

R

R

V

L

I

L

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y168 (≠ S108), T171 (≠ S111), G193 (= G128), M194 (≠ A129), I209 (≠ A144)

Sites not aligning to the query:

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 10, 12, 39, 40, 73, 74

7l81AAA Putative acetyl transferase protein (see paper)
35% identity, 75% coverage: 26:151/168 of query aligns to 108:216/216 of 7l81AAA

query
sites
7l81AAA

T

S

I

I

G

D

A

A

G

G

T

A

R

A

I

L

G

S

T

P

F

F

V

V

E

T

I

I

Q

A

K

A

N

N

V

V

V

T

V

I

G

G

R

K

D

C

C

F

K

H

I

A

S

N

S

L

H

Y

S

S

F

Y

L

V

C

-

E

-

G

-

V

-

T

-

L

-

E

E

D

H

G

D

V

C

F

I

I

I

G

G

H

D

G

Y

V

V

M

T

F

F

T
x
A

N

-

D

-

T

-

Y

-

P

P

R

R

A

-

I

V

N

S

P

C

D

N

G

G

S

N

L

I

Q

H

T

-

E

-

T

-

D

-

W

-

I

-

L

-

I

-

P

-

T

-

L

-

V

I

K

H

R

D

H

H

A

A

S
x
Y

I

I

G

G

S
x
T

N

G

A

A

T

V

I

I

L

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

I

I

G

G

E

K

A

G

A

A

Q

I

V

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

G

G

A

A

I

V

V

V

A
x
I

G

G

V

N

P

P

A

A

R

R

V

L

I

L

binding coenzyme a: A152 (≠ T76), Y168 (≠ S108), T171 (≠ S111), G193 (= G128), M194 (≠ A129), I209 (≠ A144)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 10, 12, 39, 40, 73, 74

Query Sequence

>WP_011424699.1 NCBI__GCF_000092045.1:WP_011424699.1
MIASNVKLGDGSVIHHRDLVNLYGCTIGAGTRIGTFVEIQKNVVVGRDCKISSHSFLCEG
VTLEDGVFIGHGVMFTNDTYPRAINPDGSLQTETDWILIPTLVKRHASIGSNATILPGVI
IGEAAQVGAGAVVTKDVPDGAIVAGVPARVIGRVNDGPVDMFALGEME

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory