SitesBLAST
Comparing WP_011426140.1 NCBI__GCF_000092045.1:WP_011426140.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6jw6A The crystal structure of kand2 in complex with NAD (see paper)
29% identity, 71% coverage: 6:255/353 of query aligns to 1:251/341 of 6jw6A
- binding nicotinamide-adenine-dinucleotide: G10 (= G15), F11 (≠ N16), M12 (= M17), D33 (≠ L36), L34 (≠ V37), T70 (≠ N72), T71 (= T73), P72 (≠ Y74), N73 (≠ S75), L75 (≠ S77), H76 (= H78), Q79 (≠ Y81), E93 (= E95), K94 (= K96), N122 (≠ I124), W161 (= W160), H179 (= H179)
Sites not aligning to the query:
6jw8A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin b (see paper)
29% identity, 71% coverage: 6:255/353 of query aligns to 1:251/342 of 6jw8A
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: F11 (≠ N16), F152 (≠ L151), N154 (≠ Q153), D175 (= D175), L176 (≠ C176), H179 (= H179), E236 (= E238)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G10 (= G15), F11 (≠ N16), M12 (= M17), D33 (≠ L36), L34 (≠ V37), T70 (≠ N72), T71 (= T73), P72 (≠ Y74), N73 (≠ S75), L75 (≠ S77), H76 (= H78), Q79 (≠ Y81), E93 (= E95), K94 (= K96), N122 (≠ I124), W161 (= W160), H179 (= H179)
Sites not aligning to the query:
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 290
6jw7A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin a (see paper)
29% identity, 71% coverage: 6:255/353 of query aligns to 1:251/342 of 6jw7A
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: F11 (≠ N16), N154 (≠ Q153), D175 (= D175), H179 (= H179), E236 (= E238)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G13), G10 (= G15), F11 (≠ N16), M12 (= M17), D33 (≠ L36), L34 (≠ V37), T70 (≠ N72), T71 (= T73), P72 (≠ Y74), N73 (≠ S75), L75 (≠ S77), H76 (= H78), Q79 (≠ Y81), E93 (= E95), K94 (= K96), N122 (≠ I124), W161 (= W160)
Sites not aligning to the query:
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 290
3ec7A Crystal structure of putative dehydrogenase from salmonella typhimurium lt2
27% identity, 49% coverage: 11:184/353 of query aligns to 7:181/336 of 3ec7A
- active site: K97 (= K96), H176 (= H179)
- binding nicotinamide-adenine-dinucleotide: G11 (= G15), M12 (≠ N16), I13 (≠ M17), D35 (≠ N38), I36 (≠ R39), R40 (≠ K43), T73 (≠ N72), A74 (≠ T73), S75 (≠ Y74), N76 (≠ S75), H79 (= H78), E96 (= E95), K97 (= K96), M126 (≠ I124)
Sites not aligning to the query:
7xr9A Crystal structure of dgpa with glucose (see paper)
31% identity, 37% coverage: 53:183/353 of query aligns to 48:181/344 of 7xr9A
- binding beta-D-glucopyranose: K91 (= K96), R150 (≠ Q153), W157 (= W160), F160 (≠ H163), D173 (= D175), H177 (= H179)
- binding nicotinamide-adenine-dinucleotide: D51 (= D56), Y52 (≠ F57), C67 (≠ N72), T68 (= T73), P69 (≠ Y74), H73 (= H78), E90 (= E95), K91 (= K96), P92 (= P97), Q119 (≠ I124), V159 (≠ T162), F160 (≠ H163), Q166 (≠ R168)
Sites not aligning to the query:
7xreC Crystal structure of dgpa (see paper)
31% identity, 37% coverage: 53:183/353 of query aligns to 58:191/363 of 7xreC
Sites not aligning to the query:
1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
30% identity, 56% coverage: 1:196/353 of query aligns to 28:236/383 of 1h6dA
- active site: K131 (= K96), Y219 (≠ H179)
- binding glycerol: K131 (= K96), R202 (≠ A161), D215 (= D175), Y219 (≠ H179)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G13), L41 (= L14), G42 (= G15), K43 (≠ N16), Y44 (≠ M17), S66 (≠ N38), G67 (≠ R39), K71 (= K43), Y89 (vs. gap), I107 (≠ N72), L108 (≠ T73), P109 (≠ Y74), N110 (≠ S75), H113 (= H78), E130 (= E95), K131 (= K96), R159 (≠ I124), A198 (≠ G157), W201 (= W160), R202 (≠ A161), Y219 (≠ H179)
Sites not aligning to the query:
1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
30% identity, 56% coverage: 1:196/353 of query aligns to 26:234/381 of 1rydA
- active site: K129 (= K96), Y217 (≠ H179)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (= L14), G40 (= G15), K41 (≠ N16), Y42 (≠ M17), S64 (≠ N38), G65 (≠ R39), K69 (= K43), Y87 (vs. gap), L106 (≠ T73), P107 (≠ Y74), N108 (≠ S75), L110 (≠ S77), H111 (= H78), E128 (= E95), K129 (= K96), R157 (≠ I124), A196 (≠ G157), W199 (= W160), R200 (≠ A161), Y217 (≠ H179)
Sites not aligning to the query:
1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
30% identity, 53% coverage: 11:196/353 of query aligns to 7:205/340 of 1evjA
- active site: K100 (= K96), Y188 (≠ H179)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), L10 (= L14), G11 (= G15), K12 (≠ N16), Y13 (≠ M17), D35 (≠ N38), L77 (≠ T73), P78 (≠ Y74), N79 (≠ S75), H82 (= H78), E99 (= E95), K100 (= K96), R128 (≠ I124), W170 (= W160), R171 (≠ A161), Y188 (≠ H179)
Sites not aligning to the query:
9azoA Crystal structure of chms dehydrogenase pmdc from comamonas testosteroni bound to cofactor NADP (see paper)
31% identity, 45% coverage: 5:163/353 of query aligns to 1:171/315 of 9azoA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G13), G11 (= G15), A12 (≠ N16), F13 (≠ M17), G34 (≠ N38), R35 (= R39), C71 (≠ N72), T72 (= T73), T74 (≠ S75), H77 (= H78), E94 (= E95), I95 (≠ K96), N160 (= N152), R166 (≠ P158), W168 (= W160)
Sites not aligning to the query:
4l8vA Crystal structure of a12k/d35s mutant myo-inositol dehydrogenase from bacillus subtilis with bound cofactor NADP (see paper)
28% identity, 50% coverage: 7:184/353 of query aligns to 3:181/337 of 4l8vA
Sites not aligning to the query:
3e18A Crystal structure of NAD-binding protein from listeria innocua
24% identity, 68% coverage: 5:244/353 of query aligns to 2:240/348 of 3e18A
- active site: K94 (= K96), H178 (= H179)
- binding nicotinamide-adenine-dinucleotide: G12 (= G15), G13 (≠ N16), M14 (= M17), F34 (≠ V37), D35 (≠ N38), I36 (≠ R39), K40 (= K43), A70 (≠ N72), T71 (= T73), P72 (≠ Y74), N73 (≠ S75), H76 (= H78), E93 (= E95), K94 (= K96), N122 (≠ I124), W161 (= W160), R162 (≠ A161), H178 (= H179)
3nt5A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor and product inosose (see paper)
26% identity, 50% coverage: 7:184/353 of query aligns to 3:181/337 of 3nt5A
- active site: K97 (= K96), H176 (= H179)
- binding (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone: K97 (= K96), H155 (≠ L151), H176 (= H179)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), G11 (= G15), A12 (≠ N16), I13 (≠ M17), D35 (≠ N38), V36 (≠ R39), S74 (≠ T73), W75 (≠ Y74), G76 (≠ S75), E96 (= E95), K97 (= K96)
Sites not aligning to the query:
3nt4A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor nadh and inositol (see paper)
26% identity, 50% coverage: 7:184/353 of query aligns to 3:181/337 of 3nt4A
- active site: K97 (= K96), H176 (= H179)
- binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H155 (≠ L151), H176 (= H179)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G15), A12 (≠ N16), I13 (≠ M17), D35 (≠ N38), V36 (≠ R39), S74 (≠ T73), W75 (≠ Y74), G76 (≠ S75), E96 (= E95), K97 (= K96), H176 (= H179)
Sites not aligning to the query:
3nt2B Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
26% identity, 50% coverage: 7:184/353 of query aligns to 3:181/337 of 3nt2B
- active site: K97 (= K96), H176 (= H179)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G13), G11 (= G15), A12 (≠ N16), D35 (≠ N38), V36 (≠ R39), S74 (≠ T73), W75 (≠ Y74), A78 (≠ S77), K97 (= K96)
Sites not aligning to the query:
3nt2A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
26% identity, 50% coverage: 7:184/353 of query aligns to 3:181/337 of 3nt2A
- active site: K97 (= K96), H176 (= H179)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), G11 (= G15), A12 (≠ N16), I13 (≠ M17), D35 (≠ N38), V36 (≠ R39), S74 (≠ T73), W75 (≠ Y74), G76 (≠ S75), A78 (≠ S77), H79 (= H78), E96 (= E95), K97 (= K96), H176 (= H179)
Sites not aligning to the query:
5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
31% identity, 45% coverage: 67:225/353 of query aligns to 72:233/336 of 5a06A
- active site: K101 (= K96), Y186 (≠ H179)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I77 (≠ N72), T78 (= T73), P79 (≠ Y74), N80 (≠ S75), L82 (≠ S77), H83 (= H78), E100 (= E95), K101 (= K96), R129 (≠ I124), W168 (= W160), R169 (≠ A161), Y186 (≠ H179)
- binding sorbitol: D72 (= D67), H96 (= H91), K101 (= K96), R122 (= R117), R122 (= R117), L124 (= L119), F160 (≠ Q153), R169 (≠ A161), D182 (= D175), Y186 (≠ H179)
Sites not aligning to the query:
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 11, 12, 13, 14, 36, 37, 38, 41, 59, 264
- binding sorbitol: 2, 287, 296, 299, 306, 310, 311
5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
31% identity, 45% coverage: 67:225/353 of query aligns to 72:233/336 of 5a03C
- active site: K101 (= K96), Y186 (≠ H179)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I77 (≠ N72), T78 (= T73), P79 (≠ Y74), N80 (≠ S75), L82 (≠ S77), H83 (= H78), E100 (= E95), K101 (= K96), R129 (≠ I124), W168 (= W160), R169 (≠ A161), Y186 (≠ H179)
- binding beta-D-xylopyranose: K101 (= K96), F160 (≠ Q153), R169 (≠ A161), D182 (= D175), Y186 (≠ H179)
Sites not aligning to the query:
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 14, 36, 37, 38, 41, 59, 264
5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
31% identity, 45% coverage: 67:225/353 of query aligns to 71:232/335 of 5a05A
- active site: K100 (= K96), Y185 (≠ H179)
- binding beta-D-glucopyranose: K100 (= K96), F159 (≠ Q153), D181 (= D175), Y185 (≠ H179)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I76 (≠ N72), T77 (= T73), P78 (≠ Y74), N79 (≠ S75), L81 (≠ S77), H82 (= H78), E99 (= E95), K100 (= K96), R128 (≠ I124), W167 (= W160), R168 (≠ A161), Y185 (≠ H179)
Sites not aligning to the query:
- binding alpha-D-glucopyranose: 259, 262
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 35, 36, 37, 40, 58, 263
5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
31% identity, 45% coverage: 67:225/353 of query aligns to 71:232/335 of 5a04A
- active site: K100 (= K96), Y185 (≠ H179)
- binding beta-D-glucopyranose: K100 (= K96), F159 (≠ Q153), R168 (≠ A161), D181 (= D175), Y185 (≠ H179)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I76 (≠ N72), T77 (= T73), P78 (≠ Y74), N79 (≠ S75), L81 (≠ S77), H82 (= H78), E99 (= E95), K100 (= K96), R128 (≠ I124), W167 (= W160), R168 (≠ A161), Y185 (≠ H179)
Sites not aligning to the query:
Query Sequence
>WP_011426140.1 NCBI__GCF_000092045.1:WP_011426140.1
MSQEKPIRVLVAGLGNMGRSHALAYHNDPGFEIIGLVNRSLAKLEPELRAYTVHPDFMTA
LAELKPDLCSINTYSDSHADYAVAAFEAGCHVFVEKPLATTVADAERVVAAAKKAGRKLV
IGYILRHHPSWTKLIEEARKLGPPYVFRMNLNQQSSGPTWATHKSLMRTTSPIVDCGVHY
VDVMCQITDARAVEVRGMGLRLSDEIAADMYNYGQLQVLFEDGSVGWYEAGWGPMISETA
FFVKDVISPKGAVSIVMDPDAKSDDIDTHTKTSVIRLHTAETGPDGKFIRPDQDMRMTGE
PGHQALCDLEQAFMLRAIREDLNLDRHMADAVASLRICLAADESVRTGQPVRL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory