SitesBLAST
Comparing WP_011426360.1 NCBI__GCF_000092045.1:WP_011426360.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2w54A Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus in complex with bound inhibitor pterin-6-aldehyde (see paper)
48% identity, 97% coverage: 5:476/488 of query aligns to 3:440/450 of 2w54A
- binding flavin-adenine dinucleotide: G42 (= G45), L189 (≠ V222), A191 (= A224), G192 (= G225), G193 (≠ S226), T194 (= T227), D195 (= D228), V196 (= V229), W199 (= W232), L213 (≠ I246), F258 (≠ I291), A259 (= A292), A267 (≠ G300), T268 (= T301), G271 (= G304), N272 (= N305), A274 (= A307), N275 (= N308), G280 (= G313), D281 (= D314), R318 (= R351), V324 (= V357), Q347 (≠ E382)
- binding fe2/s2 (inorganic) cluster: G38 (= G41), C39 (= C42), N40 (≠ A43), G42 (= G45), C44 (= C47), G45 (= G48), C47 (= C50), C63 (= C70), C103 (= C110), G104 (= G111), C106 (= C113), C134 (= C145), R135 (= R146), C136 (= C147)
- binding {[(5aR,8R,9aR)-2-amino-4-oxo-6,7-di(sulfanyl-kappaS)-3,5,5a,8,9a,10-hexahydro-4H-pyrano[3,2-g]pteridin-8-yl]methyl dihydrogenato(2-) phosphate}(hydroxy)oxo(thioxo)molybdenum: Q102 (= Q109), C136 (= C147)
1jroA Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus (see paper)
48% identity, 97% coverage: 5:476/488 of query aligns to 3:440/450 of 1jroA
- binding flavin-adenine dinucleotide: G42 (= G45), D43 (= D46), L189 (≠ V222), A191 (= A224), G192 (= G225), G193 (≠ S226), T194 (= T227), D195 (= D228), V196 (= V229), F258 (≠ I291), A259 (= A292), T268 (= T301), G271 (= G304), N272 (= N305), A274 (= A307), G280 (= G313), D281 (= D314), R318 (= R351), V324 (= V357)
- binding fe2/s2 (inorganic) cluster: E37 (= E40), G38 (= G41), C39 (= C42), N40 (≠ A43), G42 (= G45), C44 (= C47), G45 (= G48), C47 (= C50), C63 (= C70), C103 (= C110), C106 (= C113), C134 (= C145), C136 (= C147)
2e3tA Crystal structure of rat xanthine oxidoreductase mutant (w335a and f336l) (see paper)
34% identity, 96% coverage: 3:472/488 of query aligns to 2:493/1291 of 2e3tA
- binding flavin-adenine dinucleotide: G44 (= G45), K228 (≠ I221), L229 (≠ V222), V230 (≠ I223), V231 (≠ A224), G232 (= G225), N233 (≠ S226), T234 (= T227), E235 (≠ D228), I236 (≠ V229), L309 (≠ I291), A318 (≠ G300), S319 (≠ T301), G322 (= G304), N323 (= N305), I325 (≠ A307), T326 (≠ N308), S331 (≠ G313), D332 (= D314), I375 (≠ F356), L376 (≠ V357)
- binding fe2/s2 (inorganic) cluster: G40 (= G41), C41 (= C42), G42 (≠ A43), G44 (= G45), C46 (= C47), G47 (= G48), C49 (= C50), N69 (= N68), C71 (= C70), Q109 (= Q109), C110 (= C110), C113 (= C113), C145 (= C145), C147 (= C147)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 882, 883, 887, 888, 891
- binding calcium ion: 713, 714, 716, 809, 810, 840, 843, 844, 847, 880, 881
- binding fe2/s2 (inorganic) cluster: 717
- binding uric acid: 775, 853, 887, 982, 983, 1051, 1052, 1234
4yswA Structure of rat xanthine oxidoreductase, c-terminal deletion protein variant, nadh bound form (see paper)
34% identity, 96% coverage: 3:472/488 of query aligns to 2:491/1286 of 4yswA
- binding flavin-adenine dinucleotide: G44 (= G45), K226 (≠ I221), L227 (≠ V222), V228 (≠ I223), V229 (≠ A224), G230 (= G225), N231 (≠ S226), T232 (= T227), E233 (≠ D228), I234 (≠ V229), F307 (vs. gap), A308 (= A292), V312 (= V296), A316 (≠ G300), S317 (≠ T301), G320 (= G304), N321 (= N305), I323 (≠ A307), T324 (≠ N308), S329 (≠ G313), D330 (= D314), I373 (≠ F356), L374 (≠ V357), D399 (≠ E382)
- binding fe2/s2 (inorganic) cluster: G40 (= G41), C41 (= C42), G42 (≠ A43), G44 (= G45), C46 (= C47), G47 (= G48), C49 (= C50), C71 (= C70), C110 (= C110), G111 (= G111), C113 (= C113), C145 (= C145), C147 (= C147)
- binding 1,4-dihydronicotinamide adenine dinucleotide: E233 (≠ D228), S326 (= S310), P327 (= P311), I328 (= I312), Y363 (= Y346), R364 (≠ G347), D400 (= D383), I401 (= I384), G428 (= G410), A430 (= A412), D431 (≠ A413), P471 (≠ R453), R478 (= R459)
Sites not aligning to the query:
- active site: 738, 773, 851, 855, 883, 1231, 1232
- binding bicarbonate ion: 810, 811, 848, 880, 881, 882, 885, 886, 889
- binding calcium ion: 838, 841, 842, 845, 878, 879
- binding fe2/s2 (inorganic) cluster: 715
- binding 1,4-dihydronicotinamide adenine dinucleotide: 1196
- binding uric acid: 773, 851, 885, 980, 981, 1050, 1232
6a7xA Rat xanthine oxidoreductase, d428a variant, NAD bound form
34% identity, 96% coverage: 3:472/488 of query aligns to 2:493/1295 of 6a7xA
- binding flavin-adenine dinucleotide: G44 (= G45), K228 (≠ I221), L229 (≠ V222), V230 (≠ I223), V231 (≠ A224), G232 (= G225), N233 (≠ S226), T234 (= T227), E235 (≠ D228), I236 (≠ V229), F309 (vs. gap), V314 (= V296), S319 (≠ T301), G322 (= G304), N323 (= N305), I325 (≠ A307), T326 (≠ N308), D332 (= D314), L376 (≠ V357)
- binding fe2/s2 (inorganic) cluster: G40 (= G41), C41 (= C42), G42 (≠ A43), G44 (= G45), C46 (= C47), G47 (= G48), C49 (= C50), N69 (= N68), C71 (= C70), Q109 (= Q109), C110 (= C110), G111 (= G111), C113 (= C113), C145 (= C145), C147 (= C147)
- binding nicotinamide-adenine-dinucleotide: P329 (= P311), Y365 (= Y346), R366 (≠ G347), A432 (= A412), D433 (≠ A413), P473 (≠ R453), R480 (= R459)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 883, 884, 887, 888, 891
- binding uric acid: 775, 853, 887, 982, 983, 1052, 1234
6a7xB Rat xanthine oxidoreductase, d428a variant, NAD bound form
34% identity, 96% coverage: 3:472/488 of query aligns to 2:491/1291 of 6a7xB
- binding flavin-adenine dinucleotide: G44 (= G45), L227 (≠ V222), V228 (≠ I223), V229 (≠ A224), G230 (= G225), N231 (≠ S226), T232 (= T227), E233 (≠ D228), I234 (≠ V229), F307 (vs. gap), V312 (= V296), S317 (≠ T301), G320 (= G304), N321 (= N305), I323 (≠ A307), T324 (≠ N308), D330 (= D314), I373 (≠ F356), L374 (≠ V357)
- binding fe2/s2 (inorganic) cluster: G40 (= G41), C41 (= C42), G42 (≠ A43), G44 (= G45), C46 (= C47), G47 (= G48), C49 (= C50), N69 (= N68), C71 (= C70), Q109 (= Q109), C110 (= C110), G111 (= G111), C113 (= C113), C145 (= C145), C147 (= C147)
- binding nicotinamide-adenine-dinucleotide: P327 (= P311), Y363 (= Y346), R364 (≠ G347), G428 (= G410), A430 (= A412), D431 (≠ A413), P471 (≠ R453), R478 (= R459)
Sites not aligning to the query:
- active site: 738, 773, 851, 855, 883, 1231, 1232
- binding bicarbonate ion: 810, 811, 880, 881, 885, 886, 889
- binding fe2/s2 (inorganic) cluster: 715
- binding uric acid: 773, 851, 885, 980, 981, 1049, 1050, 1232
3zyvB Crystal structure of the mouse liver aldehyde oxidase 3 (maox3) (see paper)
33% identity, 97% coverage: 2:475/488 of query aligns to 1:485/1262 of 3zyvB
- binding flavin-adenine dinucleotide: L227 (≠ V222), I229 (≠ A224), G230 (= G225), N231 (≠ S226), T232 (= T227), Y233 (≠ D228), L234 (≠ V229), T267 (≠ A266), L303 (≠ I291), A304 (= A292), A312 (≠ G300), S313 (≠ T301), G316 (= G304), H317 (≠ N305), I319 (≠ A307), S325 (≠ G313), D326 (= D314), L366 (≠ V357), A392 (≠ D383), F393 (≠ I384)
- binding fe2/s2 (inorganic) cluster: Y39 (≠ E40), C41 (= C42), G42 (≠ A43), G44 (= G45), C46 (= C47), C49 (= C50), T69 (≠ N68), C71 (= C70), C111 (= C110), G112 (= G111), C114 (= C113), C146 (= C145), C148 (= C147)
Sites not aligning to the query:
P22985 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Rattus norvegicus (Rat) (see 2 papers)
32% identity, 96% coverage: 3:472/488 of query aligns to 4:520/1331 of P22985
- C43 (= C42) binding [2Fe-2S] cluster
- C48 (= C47) binding [2Fe-2S] cluster
- C51 (= C50) binding [2Fe-2S] cluster
- C73 (= C70) binding [2Fe-2S] cluster
- C112 (= C110) binding [2Fe-2S] cluster
- C115 (= C113) binding [2Fe-2S] cluster
- C147 (= C145) binding [2Fe-2S] cluster
- C149 (= C147) binding [2Fe-2S] cluster
- 256:263 (vs. 222:229, 25% identical) binding FAD
- WF 335:336 (vs. gap) mutation to AL: Converts the enzyme to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
- SIGGN 346:350 (≠ TIGGN 301:305) binding FAD
- D359 (= D314) binding FAD
- L403 (≠ V357) binding FAD
Sites not aligning to the query:
- 535 C→A: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with R-992. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with R-992 and S-1316.
- 992 C→R: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with A-535. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and S-1316.
- 1316 C→S: Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and R-992.
2e1qA Crystal structure of human xanthine oxidoreductase mutant, glu803val (see paper)
34% identity, 89% coverage: 3:436/488 of query aligns to 2:458/1307 of 2e1qA