SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011443665.1 NCBI__GCF_000013325.1:WP_011443665.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
39% identity, 98% coverage: 5:262/264 of query aligns to 59:311/453 of Q9VLJ8

query
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Q9VLJ8

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T62 (≠ P8) modified: Phosphothreonine

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
42% identity, 95% coverage: 11:260/264 of query aligns to 4:252/271 of Q72J02

query
sites
Q72J02

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C192 (= C196) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
41% identity, 97% coverage: 9:263/264 of query aligns to 4:251/270 of D4GSF3

query
sites
D4GSF3

A

S

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C188 (= C196) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 95% coverage: 10:260/264 of query aligns to 4:247/249 of P12282

query
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P12282

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R14 (= R20) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S50) mutation to A: No effect.
R73 (= R79) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G134) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D136) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C148) mutation to A: No effect.
C172 (= C179) mutation to A: No zinc bound and no enzyme activity.
C175 (= C182) mutation to A: No zinc bound and no enzyme activity.
C187 (= C196) mutation to A: No effect.
C231 (≠ P244) mutation to A: No effect.
C244 (= C257) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C260) mutation to A: No zinc bound and almost no enzyme activity.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
43% identity, 89% coverage: 9:243/264 of query aligns to 54:282/460 of O95396

query
sites
O95396

A

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x
L

V
x
A

G
|
G

V
x
R

A
x
P

L
|
L

S
x
V

S
|
S

V
x
A

A
x
S

L
x
A

G
x
L

R
|
R

F
|
F

Q
x
E

G
|
G

Q
|
Q

V
x
I

A
x
T

N
x
V

F
x
Y

A

-

G

-

H
|
H

R
x
Y

P
x
D

G
|
G

E
x
G

A
x
P

C
|
C

Y
|
Y

R
|
R

C
|
C

F
x
I

V
x
F

G
x
P

D
x
Q

A
x
P

F
x
P

D
x
P

A
|
A

E
|
E

D
x
T

C
x
V

D
x
T

T
x
N

C
|
C

A

A

D

D

L

G

G

G

V

V

L

L

G

G

A

V

M

V

V

T

G

G

M

V

I

L

G

G

A

C

F

L

G

Q

A

A

M

L

A

E

A

V

M

L

R

K

V

I

L

-

E

-

G

-

V

A

S

A

T

G

L

L

G

G

D

P

P

S

Q

Y

W

S

G

G

Q

S

L

L

H

L

V

L

F

F

D

D

G

A

L

L

K

R

158:238 (vs. 113:195, 39% identical) Interaction with NFS1
C239 (= C196) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
36% identity, 93% coverage: 17:262/264 of query aligns to 68:320/482 of O59954

query
sites
O59954

R

R

F

Y

A

G

R

R

H

Q

I

M

V

I

L

V

P

P

E

Q

V

F

G

G

A

L

I

Q

G
|
G

Q

Q

A

L

R

K

L

L

A

R

A

D

S

A

H

K

V

V

V

L

L

I

V

V

G

G

M

A

G

G

I

L

G
|
G

S

C

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

T

L

I

T

G

L

L

I

V

D

D

D

G

D

D

V

T

V

V

E

E

A

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

T

V

I

L

Y

H

T

R

E

S

E

R

D

N

L

V

G

G

K

K

P

L

K

K

A

V

Q

D

A

S

A

A

A

I

A

E

W

Y

V

L

A

R

R

E

F

L

D

N

P

P

A

H

L

P

E

T

V

Y

V

I

H

A

H

H

V

Q

T

A

R

H

I

L

G

T

R

P

E

R

N

E

A

A

A

P

D

D

L

I

I

F

A

K

G

D

A

Y

D

D

V

L

V

I

L

L

D

D

G
x
C

S

T

D

D

N

N

F

P

A

A

T

T

R

R

L

Y

S

L

V

I

S

S

D

D

A

T

C

A

V

V

S

L

V

L

G

G

V

K

A

P

L

L

S

V

S

S

V

A

A

S

L

A

G

L

R

R

F

T

Q
x
E

G

G

Q

Q

-

L

-

M

V

V

A

L

N

N

F

N

A

P

G

P

H

Q

R

P

P

P

G

G

E

D

A

K

-

T

-

G

-

P

C

C

Y

Y

R

R

C

C

F

V

V

F

G

P

D

K

A

P

F

P

D

P

A

A

E

N

D

S

C

V

D

T

T

S

C

C

A

A

D

D

L

G

G

G

V

I

L

L

G

G

A

P

M

V

V

V

G
|
G

M

T

I

M

G

G

A

V

F

L

G

Q

A

A

M

S

A

E

A

A

M

I

R

K

V

V

L

L

L

T

E

S

-

A

G

G

V

E

S

S

T

V

L

E

G

A

D

T

P

P

Q

P

W

-

G

-

Q

S

L

L

H

L

V

I

F

F

D

S

G

A

L

Y

-

S

K

S

P

P

S

Q

L

F

R

R

T

T

M

I

R

K

I

L

-

R

A

S

R

R

D

R

P

P

E

N

C

C

R

A

G

V

C

C

T

S

S

A

G82 (= G31) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G49) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R79) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G134) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ Q164) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G207) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
36% identity, 95% coverage: 10:260/264 of query aligns to 3:239/240 of 1jwbB

query
sites
1jwbB

L

L

T

S

P

D

D

Q

R

E

L

M

D

L

R

R

F

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

V

F

G

D

A

F

I

D

G

G

Q

Q

A

E

R

A

L

L

A

K

A

D

S

S

H

R

V

V

V

L

L

I

V

V

G
|
G

M

L

G
|
G

I

L

G

G

S

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

G

S

A

A

G

G

V

V

G

G

R

N

L

L

T

T

L

L

I

L

D
|
D

D
x
F

D

D

V

T

V

V

E

S

A

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

T

T

I

L

Y

H

T

S

E

D

E

A

D

T

L

V

G

G

K

Q

P

P

K
|
K

A

V

Q

E

A

S

A

A

A

R

A

D

W

A

V

L

A

T

R

R

F

I

D

N

P

P

A

H

L

I

E

A

V

I

V

T

H

P

H

V

V

N

T

A

R
x
L

I

L

G

D

R

D

E

A

N

E

A

L

A

A

D

A

L

L

I

I

A

A

G

E

A

H

D

D

V

L

V

V

L

L

D

D

G
x
C

S
x
T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

L

N

S

Q

V

L

S

N

D

A

A

G

C

C

V

F

S

A

V

A

G

K

V

V

A

P

L

L

S

V

S

S

V

G

A

A

L

A

G

I

R

R

F

M

Q

E

G

G

Q

Q

V

I

A

T

N

V

F

F

A

T

G

-

H

Y

R

Q

P

D

G

G

E

E

A

P

C
|
C

Y

Y

R

R

C

C

-

L

-

S

-

R

F

L

V

F

G

G

D

E

A

A

F

-

D

-

A

-

E

-

D

-

C

-

D

-

T

-

C

-

A

-

D

-

L

-

G

G

V

V

L

M

G

A

A

P

M

L

V

I

G

G

M

V

I

I

G

G

A

S

F

L

G

Q

A

A

M

M

A

E

A

A

M

I

R

K

V

M

L

L

L

-

E

-

G

-

V

-

S

A

T

G

L

Y

G

G

D

K

P

P

Q

A

W

S

G

G

Q

K

L

I

H

V

V

M

F

Y

D

D

G

A

L

M

K

T

P

C

S

Q

L

F

R

R

T

E

M

M

R

K

I

L

A

M

R

R

D

N

P

P

E

G

C
|
C

R

E

G

V

C
|
C

active site: R13 (= R20), D129 (= D136)
binding adenosine monophosphate: G37 (= G44), G39 (= G46), G40 (= G47), D61 (= D68), F62 (≠ D69), K85 (= K92), L108 (≠ R115), C127 (≠ G134), T128 (≠ S135), D129 (= D136), N130 (= N137), V133 (≠ T140)
binding zinc ion: C171 (= C179), C236 (= C257), C239 (= C260)

1jw9B Structure of the native moeb-moad protein complex (see paper)
36% identity, 95% coverage: 10:260/264 of query aligns to 3:239/240 of 1jw9B

query
sites
1jw9B

L

L

T

S

P

D

D

Q

R

E

L

M

D

L

R

R

F

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

V

F

G

D

A

F

I

D

G

G

Q

Q

A

E

R

A

L

L

A

K

A

D

S

S

H

R

V

V

V

L

L

I

V

V

G

G

M

L

G

G

I

L

G

G

S

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

G

S

A

A

G

G

V

V

G

G

R

N

L

L

T

T

L

L

I

L

D

D

D

F

D

D

V

T

V

V

E

S

A

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

T

T

I

L

Y

H

T

S

E

D

E

A

D

T

L

V

G

G

K

Q

P

P

K

K

A

V

Q

E

A

S

A

A

A

R

A

D

W

A

V

L

A

T

R

R

F

I

D

N

P

P

A

H

L

I

E

A

V

I

V

T

H

P

H

V

V

N

T

A

R

L

I

L

G

D

R

D

E

A

N

E

A

L

A

A

D

A

L

L

I

I

A

A

G

E

A

H

D

D

V

L

V

V

L

L

D

D

G

C

S

T

D
|
D

N

N

F

V

A

A

T

V

R

R

L

N

S

Q

V

L

S

N

D

A

A

G

C

C

V

F

S

A

V

A

G

K

V

V

A

P

L

L

S

V

S

S

V

G

A

A

L

A

G

I

R

R

F

M

Q

E

G

G

Q

Q

V

I

A

T

N

V

F

F

A

T

G

-

H

Y

R

Q

P

D

G

G

E

E

A

P

C
|
C

Y

Y

R

R

C

C

-

L

-

S

-

R

F

L

V

F

G

G

D

E

A

A

F

-

D

-

A

-

E

-

D

-

C

-

D

-

T

-

C

-

A

-

D

-

L

-

G

G

V

V

L

M

G

A

A

P

M

L

V

I

G

G

M

V

I

I

G

G

A

S

F

L

G

Q

A

A

M

M

A

E

A

A

M

I

R

K

V

M

L

L

L

-

E

-

G

-

V

-

S

A

T

G

L

Y

G

G

D

K

P

P

Q

A

W

S

G

G

Q

K

L

I

H

V

V

M

F

Y

D

D

G

A

L

M

K

T

P

C

S

Q

L

F

R

R

T

E

M

M

R

K

I

L

A

M

R

R

D

N

P

P

E

G

C
|
C

R

E

G

V

C
|
C

active site: R13 (= R20), D129 (= D136)
binding zinc ion: C171 (= C179), C236 (= C257), C239 (= C260)

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
34% identity, 95% coverage: 10:260/264 of query aligns to 39:289/440 of P38820

query
sites
P38820

L

L

T

S

P

L

D

E

R

E

L

Y

D

Q

R

R

F

Y

A

G

R

R

H

Q

I

M

V

I

L

V

P

E

E

E

V

T

G

G

A

G

I

V

-

A

G

G

Q

Q

A

V

R

K

L

L

A

K

A

N

S

T

H

K

V

V

V

L

L

V

V

V

G

G

M

A

G

G

I

L

G

G

S

C

P

P

A

A

L

L

Q

P

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

Q

L

I

T

G

L

I

I

V

D

D

D

N

D

D

V

V

E

E

A

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

T

V

I

L

Y

H

T

D

E

S

D

R

L

V

G

G

K

M

P

L

K

K

A

C

Q

E

A

S

A

A

A

R

A

Q

W

Y

V

I

A

T

R

K

F

L

D

N

P

P

A

H

L

I

E

N

V

V

H

T

H

Y

V

P

T

V

R

R

I

L

G

N

R

S

E

S

N

N

A

A

A

F

D

D

L

I

I

F

A

K

G

G

A

Y

D

N

V

Y

V

I

L

L

D

D

G

C

S

T

D

D

N

S

F

P

A

L

T

T

R

R

L

Y

S

L

V

V

S

S

D

D

A

V

C

A

V

V

S

N

V

L

G

G

V

I

A

T

L

V

S

V

S

S

V

A

A

S

L

G

G

L

R

G

F

T

Q

E

G

G

Q

Q

-

L

-

T

V

I

A

L

N

N

F

F

A

N

G

N

H

I

R

G

P

P

G

-

E

-

A

-

C

C

Y

Y

R

R

C

C

F

F

V

Y

G

P

D

T

A

P

F

P

D

P

A

P

E

N

D

A

C

V

D

T

T

S

C
|
C

A

Q

D

E

L

G

G

G

V

V

L

I

G

G

A

P

M

C

V

I

G

G

M

L

I

V

G

G

A

T

F

M

G

M

A

A

M

V

A

E

A

T

M

L

R

K

V

L

L

I

L

L

E

-

G

G

V

I

S

Y

T

T

L

-

G

N

D

E

P

N

Q

F

W

S

G

P

Q

F

L

L

H

M

V

L

F

Y

D

S

G

G

L

F

-

P

K

Q

P

Q

S

S

L

L

R

R

T

T

M

F

R

K

I

M

-

R

A

G

R

R

D

Q

P

E

E

K

C

C

R

L

G

C

C

C

C225 (= C196) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 92% coverage: 17:260/264 of query aligns to 8:243/251 of P30138

query
sites
P30138

R

R

F

Y

A

S

R

R

H

Q

I

I

V

L

L

L

P

D

E

D

V

I

G

A

A

L

I

D

G

G

Q

Q

A

Q

R

K

L

L

A

L

A

D

S

S

H

Q

V

V

V

L

L

I

V

I

G

G

M

L

G

G

I

L

G

G

S

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

T

L

L

T

V

L

L

I

A

D

D

V

D

V

V

E

H

A

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

T

I

I

L

Y

F

T

T

E

T

E

E

D

D

L

I

G

D

K

R

P

P

K

K

A

S

Q

Q

A

V

A

S

A

Q

W

R

V

L

A

T

R

Q

F

L

D

N

P

P

A

D

L

I

E

Q

V

L

V

T

H

A

H

L

V

Q

T

Q

R

R

I

L

G

T

R

G

E

E

N

A

A

L

A

K

D

D

L

A

I

V

A

A

G

R

A

A

D

D

V

V

L

L

D

D

G

C

S

T

D

D

N

N

F

M

A

A

T

T

R

R

L

Q

S

E

V

I

S

N

D

A

A

A

C

C

V

V

S

A

V

L

G

N

V

T

A

P

L

L

S

I

S

T

V

A

A

S

L

A

G

V

R

G

F

F

Q

G

G

G

Q

Q

V

L

A

M

N

V

F

L

A

T

G

P

H

-

R

-

P

P

G

W

E

E

A

Q

-

G

C
|
C

Y

Y

R

R

C
|
C

F

L

V
x
W

G

P

D

D

A

N

F

Q

D

E

A

P

E

E

-

R

D

N

C
|
C

D

R

T

T

C

A

A

G

D

V

L

V

G

G

V

P

L

V

G

V

A

G

M

V

V

M

G

G

M

T

I

L

G

Q

A

A

F

L

G

E

A

A

M

I

A

K

A

-

M

-

R

-

V

-

L

L

E

S

G

G

V

I

S

E

T

T

L

P

G

A

D

-

P

-

Q

-

W

-

G

G

Q

E

L

L

H

R

V

L

F

F

D

D

G

G

L

K

K

S

P

S

S

Q

L

W

R

R

T

S

M

L

R

A

I

L

A

R

R

R

D

A

P

S

E

G

C
|
C

R

P

G

V

C
|
C

C169 (= C179) binding Zn(2+)
C172 (= C182) binding Zn(2+)
W174 (≠ V184) mutation to A: No adenylation of ThiS.
C184 (= C193) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C257) binding Zn(2+)
C243 (= C260) binding Zn(2+)

1zfnA Structural analysis of escherichia coli thif (see paper)
37% identity, 92% coverage: 17:260/264 of query aligns to 8:243/244 of 1zfnA

query
sites
1zfnA

R

R

F

Y

A

S

R
|
R

H

Q

I

I

V

L

L

L

P

D

E

D

V

I

G

A

A

L

I

D

G

G

Q

Q

A

Q

R

K

L

L

A

L

A

D

S

S

H

Q

V

V

V

L

L

I

V
x
I

G
|
G

M

L

G
|
G

I

L

G

G

S

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

T

L

L

T

V

L

L

I

A

D
|
D

D

D

V

D

V

V

E

H

A

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

T

I

I

L

Y

F

T

T

E

T

E

E

D

D

L

I

G

D

K

R

P

P

K
|
K

A

S

Q

Q

A

V

A

S

A

Q

W

R

V

L

A

T

R

Q

F

L

D

N

P

P

A

D

L

I

E

Q

V

L

V

T

H

A

H

L

V

Q

T

Q

R

R

I

L

G

T

R

G

E

E

N

A

A

L

A

K

D

D

L

A

I

V

A

A

G

R

A

A

D

D

V

V

L

L

D

D

G

C

S
x
T

D
|
D

N

N

F

M

A

A

T
|
T

R

R

L

Q

S

E

V

I

S

N

D

A

A

A

C

C

V

V

S

A

V

L

G

N

V

T

A

P

L

L

S

I

S

T

V

A

A

S

L

A

G

V

R

G

F

F

Q

G

G

G

Q

Q

V

L

A

M

N

V

F

L

A

T

G

P

H

-

R

-

P

P

G

W

E

E

A

Q

-

G

C
|
C

Y

Y

R

R

C
|
C

F

L

V

W

G

P

D

D

A

N

F

Q

D

E

A

P

E

E

-

R

D

N

C

C

D

R

T

T

C

A

A

G

D

V

L

V

G

G

V

P

L

V

G

V

A

G

M

V

V

M

G

G

M

T

I

L

G

Q

A

A

F

L

G

E

A

A

M

I

A

K

A

-

M

-

R

-

V

-

L

L

E

S

G

G

V

I

S

E

T

T

L

P

G

A

D

-

P

-

Q

-

W

-

G

G

Q

E

L

L

H

R

V

L

F

F

D

D

G

G

L

K

K

S

P

S

S

Q

L

W

R

R

T

S

M

L

R

A

I

L

A

R

R

R

D

A

P

S

E

G

C
|
C

R

P

G

V

C
|
C

active site: R11 (= R20), D127 (= D136)
binding adenosine-5'-triphosphate: I34 (≠ V43), G35 (= G44), G37 (= G46), G38 (= G47), D59 (= D68), R70 (= R79), Q71 (= Q80), K83 (= K92), T126 (≠ S135), D127 (= D136), T131 (= T140)
binding zinc ion: C169 (= C179), C172 (= C182), C240 (= C257), C243 (= C260)

1zud3 Structure of this-thif protein complex (see paper)
36% identity, 92% coverage: 17:260/264 of query aligns to 8:238/240 of 1zud3

query
sites
1zud3

R

R

F

Y

A

S

R
|
R

H

Q

I

I

V
x
L

L

L

P
x
D

E

D

V

I

G

A

A

L

I

D

G

G

Q

Q

A

Q

R

K

L

L

A

L

A

D

S

S

H

Q

V

V

V

L

L

I

V

I

G

G

M

L

G

G

I

L

G

G

S

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

T

L

L

T

V

L

L

I

A

D

D

V

D

V

V

E

H

A

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

T

I

I

L

Y

F

T

T

E

T

E

E

D

D

L

I

G

D

K

R

P

P

K

K

A

S

Q

Q

A

V

A

S

A

Q

W

R

V

L

A

T

R

Q

F

L

D

N

P

P

A

D

L

I

E

Q

V

L

V

T

H

A

H

L

V

Q

T

Q

R

R

I

L

G

T

R

G

E

E

N

A

A

L

A

K

D

D

L

A

I

V

A

A

G

R

A

A

D

D

V

V

L

L

D

D

G

C

S

T

D
|
D

N

N

F

M

A

A

T

T

R

R

L

Q

S

E

V

I

S

N

D

A

A

A

C

C

V

V

S

A

V

L

G

N

V

T

A

P

L

L

S

I

S

T

V

A

A

S

L

A

G

V

R

G

F

F

Q

G

G

G

Q

Q

V

L

A

M

N

V

F

L

A

T

G

P

H

-

R

-

P

P

G

W

E

E

-

Q

A

G

C
|
C

Y

Y

R

R

C

C

F

L

V

W

G

P

D

D

A

N

F

Q

D

E

A

P

E

-

D

-

C

-

D

-

T

T

C

A

A

G

D

V

L

V

G

G

V

P

L

V

G

V

A

G

M

V

V

M

G

G

M

T

I

L

G

Q

A

A

F

L

G

E

A

A

M

I

A

K

A

-

M

-

R

-

V

-

L

L

E

S

G

G

V

I

S

E

T

T

L

P

G

A

D

-

P

-

Q

-

W

-

G

G

Q

E

L

L

H

R

V

L

F

F

D

D

G

G

L

K

K

S

P

S

S

Q

L

W

R

R

T

S

M

L

R

A

I

L

A

R

R

R

D

A

P

S

E

G

C
|
C

R

P

G

V

C
|
C

active site: R11 (= R20), D127 (= D136)
binding calcium ion: L14 (≠ V23), D16 (≠ P25)
binding zinc ion: C169 (= C179), C235 (= C257), C238 (= C260)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
33% identity, 92% coverage: 10:253/264 of query aligns to 3:217/217 of 1jwaB

query
sites
1jwaB

L

L

T

S

P

D

D

Q

R

E

L

M

D

L

R

R

F

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

V

F

G

D

A

F

I

D

G

G

Q

Q

A

E

R

A

L

L

A

K

A

D

S

S

H

R

V

V

V

L

L

I

V

V

G

G

M

L

G
|
G

I

L

G

G

S

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

G

S

A

A

G

G

V

V

G

G

R

N

L

L

T

T

L

L

I

L

D
|
D

D
x
F

D

D

V

T

V

V

E

S

A

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

T

T

I

L

Y

H

T

S

E

D

E

A

D

T

L

V

G

G

K

Q

P

P

K
|
K

A

V

Q

E

A

S

A

A

A

R

A

D

W

A

V

L

A

T

R

R

F

I

D

N

P

P

A

H

L

I

E

A

V

I

V

T

H

P

H

V

V

N

T

A

R
x
L

I

L

G

D

R

D

E

A

N

E

A

L

A

A

D

A

L

L

I

I

A

A

G

E

A

H

D

D

V

L

V

V

L

L

D

D

G

C

S

T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

L

N

S

Q

V

L

S

N

D

A

A

G

C

C

V

F

S

A

V

A

G

K

V

V

A

P

L

L

S

V

S

S

V

G

A

A

L

A

G

I

R

R

F

M

Q

E

G

G

Q

Q

V

I

A

T

N

V

F

F

A

-

G

-

H

-

R

-

P

-

G

-

E

-

A

-

C

-

Y

-

R

-

C

-

F

-

V

-

G

-

D

-

A

T

F

Y

D

E

A

A

E

-

D

-

C

-

D

-

T

-

C

-

A

-

D

-

L

-

G

G

V

V

L

M

G

A

A

P

M

L

V

I

G

G

M

V

I

I

G

G

A

S

F

L

G

Q

A

A

M

M

A

E

A

A

M

I

R

K

V

M

L

L

L

-

E

-

G

-

V

-

S

A

T

G

L

Y

G

G

D

K

P

P

Q

A

W

S

G

G

Q

K

L

I

H

V

V

M

F

Y

D

D

G

A

L

M

K

T

P

C

S

Q

L

F

R

R

T

E

M

M

R

K

I

L

A

M

R

R

active site: R13 (= R20), D129 (= D136)
binding adenosine-5'-triphosphate: G39 (= G46), G40 (= G47), D61 (= D68), F62 (≠ D69), R72 (= R79), K85 (= K92), L108 (≠ R115), D129 (= D136), N130 (= N137), V133 (≠ T140)

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
33% identity, 81% coverage: 9:222/264 of query aligns to 5:214/423 of 6yubA

query
sites
6yubA

A

S

L

L

T

S

P

K

D

E

R

E

L

I

D

E

R

R

F

Y

A

S

R

R

H

Q

I

M

V

I

L

V

P

P

E

G

V

M

G

G

A

K

I

E

G

G

Q

Q

A

L

R

R

L

L

A

M

A

N

S

A

H

K

V

V

V

L

L

I

V

I

G

G

M

A

G

G

I

L

G

G

S

C

P

P

A

A

L

A

Q

Q

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

T

L

I

T

G

L

I

I

V

D

D

D

G

D

D

V

S

V

V

E

E

A

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

T

V

I

A

Y

H

T

A

E

T

E

K

D

R

L

V

G

G

K

M

P

L

K

K

A

V

Q

D

A

S

A

L

A

I

A

T

W

H

V

L

A

I

R

E

F

I

D

N

P

P

A

L

L

P

E

V

V

Y

V

V

H

P

H

Y

V

R

T

F

R

D

I

L

G

T

R

P

E

Q

N

N

A

A

D

Q

L

I

I

I

A

K

G

P

A

W

D

D

V

V

V

I

L

L

D

D

G

C

S

T

D

D

N

N

F

P

A

A

T

T

R

R

L

Y

S

L

V

I

S

S

D

D

A

V

C

C

V

V

S

L

V

L

G

G

V

K

A

P

L

L

S

V

S

S

V

A

A

A

L

S

G

V

R

Q

F

K

Q

S

G

G

Q

Q

V

L

A

I

N

V

F

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

A

V

G

V

H

N

R

K

P

K

G

A

E

A

A

P

C
|
C

Y

Y

R

R

C
|
C

F

C

V

F

G

K

D

K

A

P

F

-

D

-

A

-

E

-

D

-

C

-

D

-

T

-

C

-

A

-

D

-

L

-

G

G

V

I

L

M

G

G

A

P

M

V

V

V

G

G

M

M

I

M

G

G

A

V

F

A

G

Q

A

A

M

G

A

E

A

A

M

I

R

K

V

I

L

L

L

V

binding zinc ion: C183 (= C179), C186 (= C182)

Sites not aligning to the query:

binding zinc ion: 266, 269

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
35% identity, 81% coverage: 9:222/264 of query aligns to 6:213/289 of 6yubB

query
sites
6yubB

A

S

L

L

T

S

P

K

D

E

R

E

L

I

D

E

R

R

F

Y

A

S

R

R

H

Q

I

M

V

I

L

V

P

P

E

G

V

M

G

G

A

K

I

E

G

G

Q

Q

A

L

R

R

L

L

A

M

A

N

S

A

H

K

V

V

V

L

L

I

V

I

G

G

M

A

G

G

I

L

G

G

S

C

P

P

A

A

L

A

Q

Q

Y

Y

L

L

A

A

G

G

A

A

G

G

V

V

G

G

R

T

L

I

T

G

L

I

I

V

D

D

D

G

D

D

V

S

V

V

E

E

A

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

T

V

I

A

Y

H

T

A

E

T

E

K

D

R

L

V

G

G

K

M

P

L

K

K

A

V

Q

D

A

S

A

L

A

I

A

T

W

H

V

L

A

I

R

E

F

I

D

N

P

P

A

L

L

P

E

V

V

Y

V

V

H

P

H

Y

V

R

T

F

R

D

I

L

G

T

R

P

E

Q

N

N

A

A

D

Q

L

I

I

I

A

K

G

P

A

W

D

D

V

V

V

I

L

L

D

D

G

C

S

T

D

D

N

N

F

P

A

A

T

T

R

R

L

Y

S

L

V

I

S

S

D

D

A

V

C

C

V

V

S

L

V

L

G

G

V

K

A

P

L

L

S

V

S

S

V

A

A

A

L

S

G

V

R

Q

F

K

Q

S

G

G

Q

Q

-

L

-

I

V

V

A

L

N

N

F

C

A

P

G

P

H

T

R

P

P

Q

G

G

E

V

A

V

C

N

Y

K

R

K

C

-

F

-

V

-

G

-

D

-

A

-

F

-

D

-

A

A

E

A

D

P

C
|
C

D

Y

T

R

C
|
C

A

C

D

F

L

K

G

G

V

I

L

M

G

G

A

P

M

V

V

V

G

G

M

M

I

M

G

G

A

V

F

A

G

Q

A

A

M

G

A

E

A

A

M

I

R

K

V

I

L

L

L

V

binding zinc ion: C184 (= C193), C187 (= C196)

Sites not aligning to the query:

binding zinc ion: 265

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
30% identity, 56% coverage: 35:182/264 of query aligns to 16:162/650 of Q7SXG4

query
sites
Q7SXG4

L

L

A

S

S

C

H

R

V

V

V

L

L

V

V

V

G

G

M

A

G

G

I

I

G

G

S

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

G

L

A

T

G

G

V

F

G

K

R

N

L

I

T

E

L

V

I

I

D

D

D

L

D

D

V

T

V

I

E

D

A

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

T

F

I

L

Y

F

T

Q

E

K

D

H

L

V

G

G

K

K

P

S

K

K

A

A

Q

Q

A

V

A

A

A

K

A

E

W

S

V

V

A

L

R

R

F

F

D

C

P

P

A

S

L

A

E

N

V

I

V

T

-

A

H

Y

H

H

V

D

T

S

R

I

I

M

G

N

R

P

E

D

N

Y

A

N

A

V

D

E

L

F

I

F

A

R

G

N

A

F

D

Q

V

L

V

V

L

M

D

N

G

A

S

L

D

D

N

N

F

R

A

A

T

A

R

R

L

N

S

H

V

V

S

N

D

R

A

M

C

C

V

L

S

A

V

A

G

D

V

I

A

P

L

L

S

I

S

E

V

S

A

G

L

T

G

A

R

G

F

Y

Q

L

G

G

Q

Q

V

V

A

T

N

V

F

I

A

-

G

-

H

-

R

K

P

K

G

G

E

Q

A

T

-

E

C

C

Y

Y

R

E

C

C

Sites not aligning to the query:

599 modified: Phosphoserine

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 56% coverage: 35:182/264 of query aligns to 23:169/628 of O42939

query
sites
O42939

L

F

A

K

A

S

S

A

H

K

V

V

V

L

L

L

V

V

G

G

M

A

G

G

I

I

G

G

S

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

L

G

M

A

S

G

G

V

V

G

K

R

E

L

V

T

H

L

I

I

I

D

D

D

L

D

D

V

T

V

I

E

D

A

L

S

S

N

N

L

L

Q

N

R

R

Q

Q

T

F

I

L

Y

F

T

R

E

K

D

H

L

V

G

K

K

Q

P

P

K

K

A

A

Q

I

A

V

A

A

A

K

W

T

V

A

A

S

R

S

F

F

D

N

P

P

-

N

-

V

A

K

L

L

E

E

V

A

V

Y

H

H

H

A

V

N

T

I

R

K

I

E

G

D

R

R

E

F

N

N

A

V

A

A

D

-

L

W

I

F

A

R

G

Q

A

F

D

D

V

L

V

V

L

F

D

N

G

A

S

L

D

D

N

N

F

L

A

D

T

A

R

R

L

R

S

H

V

V

S

N

D

K

A

Q

C

C

V

L

S

L

V

A

G

S

V

V

A

P

L

L

S

I

S

E

V

S

A

G

L

T

G

T

R

G

F

F

Q

L

G

G

Q

Q

V

V

A

Q

N

-

F

V

A

I

G

I

H

H

R

G

P

K

G

T

E

E

A

-

C

C

Y

Y

R

D

C

C

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

6xohB Structure of sumo1-ml00789344 adduct bound to sae (see paper)
30% identity, 58% coverage: 35:188/264 of query aligns to 10:162/490 of 6xohB

query
sites
6xohB

L

V

A

A

A

G

S

G

H

R

V

V

V

L

L

V

V

V

G

G

M

A

G

G

I

I

G

G

S

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

G

L

A

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

D
x
L

D

D

V

T

V

I

E

D

A

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

T

F

I

L

Y

F

T

Q

E

K

D

H

L

V

G

G

K

R

P

S

K
|
K

A

A

Q

Q

A

V

A

A

A

K

A

E

W

S

V

V

A

L

R

Q

F

F

D

Y

P

P

A

K

L

A

E

N

V

I

V

V

-

A

H

Y

H

H

V

D

T
x
S

R
x
I

I
x
M

G

N

R

P

E

D

N

Y

A

N

A

V

D

E

L

F

I

F

A

R

G

Q

A

F

D

I

V

L

V

V

L

M

D

N

G

A

S
x
L

D
|
D

N
|
N

F

R

A

A

T

A

R

R

L

N

S

H

V

V

S

N

D

R

A

M

C

C

V

L

S

A

V

A

G

D

V

V

A

P

L

L

S

I

S

E

V

S

A

G

L

T

G

A

R

G

F

Y

Q

L

G

G

Q

Q

V

V

A

T

N

T

F

I

A

K

G

-

H

-

R

K

P

G

G

V

E

T

A

E

C
|
C

Y

Y

R

E

C
|
C

F

H

V

P

G

K

D

R

A

T

F

F

active site: D112 (= D136)
binding {(1R,2S,4R)-4-[(5-{4-[(1R)-3,4-dihydro-1H-2-benzopyran-1-yl]thiophene-2-carbonyl}pyrimidin-4-yl)amino]-2-hydroxycyclopentyl}methyl sulfamate: D43 (= D68), L44 (≠ D69), K67 (= K92), S90 (≠ T114), I91 (≠ R115), M92 (≠ I116), L111 (≠ S135), D112 (= D136), N113 (= N137)
binding zinc ion: C153 (= C179), C156 (= C182)

Sites not aligning to the query:

active site: 165, 166, 168
binding zinc ion: 391, 394

6xogB Structure of sumo1-ml786519 adduct bound to sae (see paper)
30% identity, 58% coverage: 35:188/264 of query aligns to 8:160/500 of 6xogB

query
sites
6xogB

L

V

A

A

A

G

S

G

H

R

V

V

V

L

L

V

V

V

G

G

M

A

G

G

I

I

G

G

S

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

G

L

A

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

D
x
L

D

D

V

T

V

I

E

D

A

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

T

F

I

L

Y

F

T

Q

E

K

D

H

L

V

G

G

K

R

P

S

K
|
K

A

A

Q

Q

A

V

A

A

A

K

A

E

W

S

V

V

A

L

R

Q

F

F

D

Y

P

P

A

K

L

A

E

N

V

I

V

V

-

A

H

Y

H

H

V

D

T
x
S

R
x
I

I
x
M

G

N

R

P

E

D

N

Y

A

N

A

V

D

E

L

F

I

F

A

R

G

Q

A

F

D

I

V

L

V

V

L

M

D

N

G
x
A

S

L

D
|
D

N
|
N

F

R

A

A

T

A

R

R

L

N

S

H

V

V

S

N

D

R

A

M

C

C

V

L

S

A

V

A

G

D

V

V

A

P

L

L

S

I

S

E

V

S

A

G

L

T

G

A

R

G

F

Y

Q

L

G

G

Q

Q

V

V

A

T

N

T

F

I

A

K

G

-

H

-

R

K

P

G

G

V

E

T

A

E

C
|
C

Y

Y

R

E

C
|
C

F

H

V

P

G

K

D

P

A

T

F

F

active site: D110 (= D136)
binding {(1R,2R,3S,4R)-4-[(5-{4-[(1S)-1-(6-bromopyridin-2-yl)-1-hydroxyethyl]thiophene-2-carbonyl}pyrimidin-4-yl)amino]-2,3-dihydroxycyclopentyl}methyl sulfamate: D41 (= D68), L42 (≠ D69), K65 (= K92), S88 (≠ T114), I89 (≠ R115), M90 (≠ I116), A108 (≠ G134), D110 (= D136), N111 (= N137)
binding zinc ion: C151 (= C179), C154 (= C182)

Sites not aligning to the query:

active site: 163, 164, 166
binding zinc ion: 398, 401

6xoiB Structure of sumo1-ml00752641 adduct bound to sae (see paper)
30% identity, 56% coverage: 35:182/264 of query aligns to 8:154/403 of 6xoiB

query
sites
6xoiB

L

V

A

A

A

G

S

G

H

R

V

V

V

L

L

V

V

V

G

G

M

A

G

G

G
|
G

I

I

G

G

S

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

G

L

A

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

D
x
L

D

D

V

T

V

I

E

D

A

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

T

F

I

L

Y

F

T

Q

E

K

D

H

L

V

G

G

K

R

P

S

K
|
K

A

A

Q

Q

A

V

A

A

A

K

A

E

W

S

V

V

A

L

R

Q

F

F

D

Y

P

P

A

K

L

A

E

N

V

I

V

V

-

A

H

Y

H

H

V

D

T
x
S

R
x
I

I

M

G

N

R

P

E

D

N

Y

A

N

A

V

D

E

L

F

I

F

A

R

G

Q

A

F

D

I

V

L

V

V

L

M

D

N

G
x
A

S
x
L

D
|
D

N
|
N

F

R

A

A

T

A

R

R

L

N

S

H

V

V

S

N

D

R

A

M

C

C

V

L

S

A

V

A

G

D

V

V

A

P

L

L

S

I

S

E

V

S

A

G

L

T

G

A

R

G

F

Y

Q

L

G

G

Q

Q

V

V

A

T

N

T

F

I

A

-

G

-

H

K

R

K

P

G

G

V

E

T

A

E

C
|
C

Y

Y

R

E

C
|
C

active site: D110 (= D136)
binding [(1R,2R,3S,4R)-4-{[5-(1-benzyl-1H-pyrazole-3-carbonyl)pyrimidin-4-yl]amino}-2,3-dihydroxycyclopentyl]methyl sulfamate: G20 (= G47), D41 (= D68), L42 (≠ D69), K65 (= K92), S88 (≠ T114), I89 (≠ R115), A108 (≠ G134), L109 (≠ S135), D110 (= D136), N111 (= N137)
binding zinc ion: C151 (= C179), C154 (= C182)

Sites not aligning to the query:

active site: 159, 160, 162
binding zinc ion: 353, 356

Query Sequence

>WP_011443665.1 NCBI__GCF_000013325.1:WP_011443665.1
MTTPALSPALTPDRLDRFARHIVLPEVGAIGQARLAASHVVLVGMGGIGSPALQYLAGAG
VGRLTLIDDDVVEASNLQRQTIYTEEDLGKPKAQAAAAWVARFDPALEVVHHVTRIGREN
AADLIAGADVVLDGSDNFATRLSVSDACVSVGVALSSVALGRFQGQVANFAGHRPGEACY
RCFVGDAFDAEDCDTCADLGVLGAMVGMIGAFGAMAAMRVLLEGVSTLGDPQWGQLHVFD
GLKPSLRTMRIARDPECRGCTSGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory