SitesBLAST
Comparing WP_011445679.1 NCBI__GCF_000013325.1:WP_011445679.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
44% identity, 96% coverage: 17:482/487 of query aligns to 2:457/471 of P04805
- C98 (= C113) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (≠ L115) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (≠ W140) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ D142) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ T144) mutation to Q: No change in activity or in zinc content.
- H131 (≠ Q147) mutation to Q: No change in activity or in zinc content.
- H132 (≠ W148) mutation to Q: No change in activity or in zinc content.
- C138 (≠ Y154) mutation to S: No change in activity or in zinc content.
- S239 (= S260) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
44% identity, 96% coverage: 17:482/487 of query aligns to 2:457/468 of 8i9iA
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
47% identity, 77% coverage: 18:390/487 of query aligns to 3:353/380 of 4g6zA
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of 1g59A
- binding : D44 (= D60), R45 (= R61), A46 (= A62), R47 (= R63), P109 (≠ Q117), V145 (= V155), R163 (≠ Q173), V166 (≠ I176), E172 (= E182), V177 (≠ I187), K180 (≠ R190), S181 (= S191), D182 (= D192), E207 (≠ D217), E208 (≠ D218), R237 (≠ H252), K241 (≠ G256), T242 (≠ A257), K243 (= K258), M273 (≠ L289), G274 (= G290), E282 (= E297), S299 (≠ N314), L300 (≠ K315), P303 (≠ S318), V304 (≠ R319), K309 (= K324), W312 (≠ N327), R319 (= R334), P357 (≠ V374), R358 (= R375), R417 (≠ K436), K426 (≠ G445), L427 (= L446), Q432 (= Q451), R435 (= R454), L442 (≠ T461), E443 (≠ T462), T444 (≠ S463), P445 (= P464), G446 (= G465), L447 (≠ I466), F448 (= F467)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of 2cv2A
- active site: K246 (= K261)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R21), A7 (= A23), S9 (= S25), G17 (= G33), I21 (≠ T37), E41 (= E57), Y187 (= Y197), R205 (= R215), A206 (≠ G216), E208 (≠ D218), W209 (≠ H219), L235 (= L250), L236 (≠ I251)
- binding : S9 (= S25), T43 (= T59), D44 (= D60), R47 (= R63), V145 (= V155), R163 (≠ Q173), Y168 (≠ V178), E172 (= E182), V177 (≠ I187), K180 (≠ R190), S181 (= S191), Y187 (= Y197), E207 (≠ D217), E208 (≠ D218), W209 (≠ H219), V211 (≠ N221), R237 (≠ H252), K241 (≠ G256), L272 (≠ R288), M273 (≠ L289), G274 (= G290), E282 (= E297), S299 (≠ N314), P303 (≠ S318), V304 (≠ R319), K309 (= K324), W312 (≠ N327), R319 (= R334), P357 (≠ V374), R358 (= R375), R417 (≠ K436), Q432 (= Q451), R435 (= R454), L442 (≠ T461), E443 (≠ T462), T444 (≠ S463), G446 (= G465), L447 (≠ I466), F448 (= F467)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of 2cv1A
- active site: K246 (= K261)
- binding adenosine-5'-triphosphate: P8 (= P24), S9 (= S25), G17 (= G33), T18 (≠ G34), I21 (≠ T37), R47 (= R63), A206 (≠ G216), W209 (≠ H219), L235 (= L250), L236 (≠ I251)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R21), A7 (= A23), E41 (= E57), Y187 (= Y197), R205 (= R215), W209 (≠ H219)
- binding : S9 (= S25), E41 (= E57), T43 (= T59), D44 (= D60), R47 (= R63), V145 (= V155), R163 (≠ Q173), V166 (≠ I176), E172 (= E182), V177 (≠ I187), K180 (≠ R190), S181 (= S191), Y187 (= Y197), E207 (≠ D217), E208 (≠ D218), W209 (≠ H219), V211 (≠ N221), R237 (≠ H252), K241 (≠ G256), K243 (= K258), M273 (≠ L289), G274 (= G290), S276 (≠ G292), E282 (= E297), S299 (≠ N314), P303 (≠ S318), V304 (≠ R319), K309 (= K324), W312 (≠ N327), R319 (= R334), P357 (≠ V374), R358 (= R375), R417 (≠ K436), L427 (= L446), Q432 (= Q451), R435 (= R454), L442 (≠ T461), E443 (≠ T462), T444 (≠ S463), G446 (= G465), L447 (≠ I466), F448 (= F467)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of 1n78A
- active site: K246 (= K261)
- binding glutamol-amp: R5 (= R21), A7 (= A23), P8 (= P24), S9 (= S25), G17 (= G33), T18 (≠ G34), I21 (≠ T37), E41 (= E57), Y187 (= Y197), N191 (≠ V201), R205 (= R215), A206 (≠ G216), E208 (≠ D218), W209 (≠ H219), L235 (= L250), L236 (≠ I251)
- binding : S9 (= S25), T43 (= T59), D44 (= D60), R47 (= R63), V145 (= V155), R163 (≠ Q173), V166 (≠ I176), Y168 (≠ V178), E172 (= E182), V177 (≠ I187), K180 (≠ R190), S181 (= S191), Y187 (= Y197), E207 (≠ D217), E208 (≠ D218), W209 (≠ H219), L210 (≠ I220), V211 (≠ N221), R237 (≠ H252), K241 (≠ G256), M273 (≠ L289), G274 (= G290), E282 (= E297), R297 (≠ D312), P303 (≠ S318), V304 (≠ R319), K309 (= K324), W312 (≠ N327), R319 (= R334), P357 (≠ V374), R358 (= R375), R417 (≠ K436), L427 (= L446), Q432 (= Q451), R435 (= R454), L442 (≠ T461), E443 (≠ T462), T444 (≠ S463), G446 (= G465), L447 (≠ I466), F448 (= F467)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of 1j09A
- active site: K246 (= K261)
- binding adenosine-5'-triphosphate: H15 (= H31), E208 (≠ D218), L235 (= L250), L236 (≠ I251), K243 (= K258), I244 (≠ L259), S245 (= S260), K246 (= K261), R247 (= R262)
- binding glutamic acid: R5 (= R21), A7 (= A23), S9 (= S25), E41 (= E57), Y187 (= Y197), N191 (≠ V201), R205 (= R215), W209 (≠ H219)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
38% identity, 95% coverage: 18:482/487 of query aligns to 2:463/468 of P27000
- R358 (= R375) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
34% identity, 95% coverage: 20:483/487 of query aligns to 4:476/485 of 4griB
- active site: S9 (= S25), K253 (= K261)
- binding glutamic acid: R5 (= R21), A7 (= A23), S9 (= S25), E41 (= E57), Y194 (= Y197), R212 (= R215), W216 (≠ H219)
- binding zinc ion: C105 (= C113), C107 (≠ L115), Y128 (≠ I136), C132 (≠ W140)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
34% identity, 95% coverage: 18:482/487 of query aligns to 103:555/564 of 3al0C
- active site: S110 (= S25), K335 (= K261)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R21), A108 (= A23), P109 (= P24), G118 (= G33), T122 (= T37), E142 (= E57), Y276 (= Y197), R294 (= R215), G295 (= G216), D297 (= D218), H298 (= H219), L324 (= L250), I325 (= I251), L333 (= L259)
- binding : T144 (= T59), D145 (= D60), R148 (= R63), Y208 (≠ Q117), P213 (≠ A122), K252 (≠ Q173), M255 (≠ I176), I266 (= I187), K269 (≠ R190), S270 (= S191), Y276 (= Y197), D297 (= D218), H298 (= H219), L299 (≠ I220), S300 (≠ N221), N301 (= N222), K304 (≠ R225), R330 (≠ G256), P332 (≠ K258), G363 (= G290), W364 (= W291), R365 (≠ G292), E370 (= E297), S387 (≠ N314), K389 (≠ G316), V391 (≠ S318), I392 (≠ R319), K397 (= K324), W400 (≠ N327), R407 (= R334), E446 (≠ V374), K447 (≠ R375), Q453 (≠ E381), I457 (≠ S386), R509 (≠ K436), K520 (= K448), Q524 (= Q451), R527 (= R454), V535 (≠ T462), T536 (≠ S463), G538 (= G465), L539 (≠ I466)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
33% identity, 96% coverage: 18:484/487 of query aligns to 4:464/488 of 8vc5A
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
37% identity, 94% coverage: 18:477/487 of query aligns to 2:470/484 of 2cfoA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
37% identity, 94% coverage: 18:477/487 of query aligns to 3:471/485 of Q8DLI5
- R6 (= R21) binding L-glutamate
- Y192 (= Y197) binding L-glutamate
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
31% identity, 96% coverage: 16:482/487 of query aligns to 1:494/502 of 6brlA
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
35% identity, 51% coverage: 17:266/487 of query aligns to 3:234/290 of 4a91A
- active site: S11 (= S25), K229 (= K261)
- binding glutamic acid: R7 (= R21), A9 (= A23), S11 (= S25), E43 (= E57), Y170 (= Y197), R188 (= R215), L192 (≠ H219)
- binding zinc ion: C99 (= C113), C101 (≠ L115), Y113 (≠ A127), C117 (≠ R131)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
35% identity, 51% coverage: 17:265/487 of query aligns to 15:245/308 of P27305
- E55 (= E57) binding L-glutamate
- Y182 (= Y197) binding L-glutamate
- R200 (= R215) binding L-glutamate
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
32% identity, 57% coverage: 15:290/487 of query aligns to 8:278/455 of 3aiiA
8uk6A Candida albicans glutaminyl tRNA synthetase (gln4) in complex with n- pyrimidinyl-beta-thiophenylacrylamide (see paper)
36% identity, 23% coverage: 16:128/487 of query aligns to 37:148/573 of 8uk6A
Sites not aligning to the query:
Query Sequence
>WP_011445679.1 NCBI__GCF_000013325.1:WP_011445679.1
MASATDTVSAGSTPRDKVVTRFAPSPTGFLHIGGARTALFNWLYARHHGGTYLLRIEDTD
RARSTDAAIDAIFDGLEWLGLGGDEPAVFQFARSDRHAEVAHKLLEAGHAYRCYLTQEEL
AARRELAQAERRPFRIDSEWRDATPDQWPADQSYVVRMKAPREGETTIVDKVQGSITVQN
SELDDFIILRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHINNAFRQLVIIRGMHAIEGGW
PDPVYAHIPLIHGADGAKLSKRHGALGVDAYRDEMGLLPEAVFNYLLRLGWGHGDEEIIS
REQAVAWFDIGDVNKGASRFDLKKLLNLNGHYIREADDARLAALVAPRLATLAPGFAPDK
GLDLLTRAMPVLKVRAADINELAAGSVFLFAQRPLAMAEKAASLLTDDARAILTKVAGVL
EAENVWTTGVLEATVKQMAEELGIGLGKIAQPLRASLTGQTTSPGIFDVLALLGKDESLS
RIRDQAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory