SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011446953.1 NCBI__GCF_000013325.1:WP_011446953.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
44% identity, 96% coverage: 8:456/467 of query aligns to 12:454/463 of P26276

query
sites
P26276

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R15 (= R11) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
Y17 (= Y13) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
R20 (= R16) mutation to A: No phosphoglucomutase activity.
S108 (= S105) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
N110 (= N107) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
D242 (= D244) binding Mg(2+)
D244 (= D246) binding Mg(2+)
D246 (= D248) binding Mg(2+)
R247 (= R249) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
R262 (≠ Q264) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
K285 (= K287) binding alpha-D-glucose 1-phosphate
H308 (= H310) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
E325 (= E327) mutation to A: Reduces KM and Vmax approximately 2-fold.
EMSGH 325:329 (≠ EMTGH 327:331) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
H329 (= H331) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
P368 (= P370) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
R421 (= R423) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
RASNT 421:425 (= RASNT 423:427) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 12:454/463 of Q02E40

query
sites
Q02E40

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S108 (= S105) active site, Non-phosphorylated intermediate; modified: Phosphoserine

1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 7:449/458 of 1pcjX

query
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1pcjX

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T

G
|
G

H

H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E
|
E

M

M

T
x
S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: R15 (= R16), S103 (= S105), H104 (= H106), K113 (= K115), D237 (= D244), D239 (= D246), D241 (= D248), R242 (= R249), H324 (= H331), D335 (= D342)
binding 1-O-phosphono-alpha-D-mannopyranose: Y12 (= Y13), S103 (= S105), T301 (= T308), G302 (= G309), E320 (= E327), S322 (≠ T329), H324 (= H331), R416 (= R423), S418 (= S425), N419 (= N426), T420 (= T427)
binding zinc ion: S103 (= S105), D237 (= D244), D239 (= D246), D241 (= D248)

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 4:446/455 of 2h5aX

query
sites
2h5aX

S

S

I

I

L

F

R

R

E

A

Y
|
Y

D

D

I

I

R

R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K

K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E
|
E

M

M

T
x
S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: H101 (= H106), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), D332 (= D342)
binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (= Y13), T298 (= T308), G299 (= G309), H300 (= H310), E317 (= E327), S319 (≠ T329), H321 (= H331), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S105), D234 (= D244), D236 (= D246), D238 (= D248)

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 4:446/455 of 2h4lX

query
sites
2h4lX

S

S

I

I

L

F

R

R

E

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K

K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T
|
T

G

G

H

H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E
|
E

M

M

T

S

G

G

H

H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: H101 (= H106), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), D332 (= D342)
binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (= Y13), R12 (= R16), S100 (= S105), T298 (= T308), E317 (= E327), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S105), D234 (= D244), D236 (= D246), D238 (= D248)

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 4:446/455 of 2fkfA

query
sites
2fkfA

S

S

I

I

L

F

R
|
R

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K
|
K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E

E

M

M

T
x
S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: R12 (= R16), S100 (= S105), H101 (= H106), K110 (= K115), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), H321 (= H331), D332 (= D342)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (= R11), H101 (= H106), S319 (≠ T329), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S105), D234 (= D244), D236 (= D246), D238 (= D248)

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 4:446/455 of 1pcmX

query
sites
1pcmX

S

S

I

I

L

F

R

R

E

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K
|
K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E
|
E

M

M

T
x
S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N

N

T

T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: R12 (= R16), S100 (= S105), H101 (= H106), K110 (= K115), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), H321 (= H331), D332 (= D342)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y13), S100 (= S105), T298 (= T308), G299 (= G309), H300 (= H310), E317 (= E327), S319 (≠ T329), H321 (= H331), R413 (= R423), S415 (= S425)
binding zinc ion: S100 (= S105), D234 (= D244), D236 (= D246), D238 (= D248)

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 4:446/455 of 1p5gX

query
sites
1p5gX

S

S

I

I

L

F

R

R

E

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K
|
K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K
|
K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T

T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E
|
E

M

M

T
x
S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: R12 (= R16), S100 (= S105), H101 (= H106), K110 (= K115), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), H321 (= H331), D332 (= D342)
binding 6-O-phosphono-alpha-D-glucopyranose: Y9 (= Y13), S100 (= S105), K277 (= K287), G299 (= G309), H300 (= H310), E317 (= E327), S319 (≠ T329), H321 (= H331), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S105), D234 (= D244), D236 (= D246), D238 (= D248)

1p5dX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 4:446/455 of 1p5dX

query
sites
1p5dX

S

S

I

I

L

F

R

R

E

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K
|
K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E
|
E

M

M

T
x
S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R
|
R

A

A

S
|
S

N

N

T
|
T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: R12 (= R16), S100 (= S105), H101 (= H106), K110 (= K115), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), H321 (= H331), D332 (= D342)
binding 1-O-phosphono-alpha-D-glucopyranose: Y9 (= Y13), S100 (= S105), R239 (= R249), T298 (= T308), G299 (= G309), H300 (= H310), E317 (= E327), S319 (≠ T329), H321 (= H331), R413 (= R423), S415 (= S425), T417 (= T427)
binding zinc ion: S100 (= S105), D234 (= D244), D236 (= D246), D238 (= D248)

1k2yX Crystal structure of phosphomannomutase/phosphoglucomutase s108a mutant from p. Aeruginosa (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 8:450/459 of 1k2yX

query
sites
1k2yX

S

S

I

I

L

F

R

R

E

A

Y

Y

D

D

I

I

R

R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S

A

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K

K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E

E

M

M

T

S

G

G

H

H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R

R

A

A

S

S

N

N

T

T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: H105 (= H106), D238 (= D244), D240 (= D246), D242 (= D248), R243 (= R249), D336 (= D342)
binding zinc ion: D238 (= D244), D240 (= D246), D242 (= D248)

4il8A Crystal structure of an h329a mutant of p. Aeruginosa pmm/pgm (see paper)
44% identity, 96% coverage: 8:456/467 of query aligns to 8:450/459 of 4il8A

query
sites
4il8A

S

S

I

I

L

F

R

R

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

V

F

V

G

G

E

D

T

T

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K
|
K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E

E

M

M

T

S

G

G

H
x
A

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R

R

A

A

S

S

N

N

T

T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: R16 (= R16), S104 (= S105), H105 (= H106), K114 (= K115), D238 (= D244), D240 (= D246), D242 (= D248), R243 (= R249), A325 (≠ H331), D336 (= D342)
binding magnesium ion: S104 (= S105), D238 (= D244), D240 (= D246), D242 (= D248)

3uw2A X-ray crystal structure of phosphoglucomutase/phosphomannomutase family protein (bth_i1489)from burkholderia thailandensis (see paper)
43% identity, 94% coverage: 8:446/467 of query aligns to 5:439/458 of 3uw2A

query
sites
3uw2A

S

S

I

I

L

F

R

K

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

V

F

I

G

G

E

K

T

T

L

L

E

D

V

A

E

D

D

V

A

A

R

R

A

S

V

I

G

G

R

R

S

A

F

F

G

G

T

S

M

E

V

V

A

R

R

A

R

Q

G

G

H

D

R

A

V

V

G

A

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

G

A

A

L

L

V

A

E

D

G

G

L

L

V

R

A

A

S

A

G

G

I

V

D

D

V

V

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

G

Y

Y

Y

F

A

A

E

-

A

A

S

S

M

V

P

P

-

L

-

A

-

L

-

S

-

G

-

E

-

R

E

R

V

V

Q

D

G

S

G

C

V

I

Q

V

V

V

T

T

G

G

S
|
S

H
|
H

N

N

P

P

A

P

D

D

H

Y

N

N

G

G

F

F

K
|
K

L

M

V

V

F

L

E

R

G

G

S

A

A

A

L

I

F

Y

G

G

T

D

D

Q

I

I

F

Q

D

G

L

L

G

Y

R

K

L

R

A

I

G

V

E

D

G

A

D

R

W

F

I

E

S

T

G

G

S

S

G

G

R

S

V

Y

E

E

E

Q

A

Y

D

D

V

V

L

A

P

D

G

Q

Y

Y

V

V

E

E

R

R

L

I

L

V

Q

G

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

K

A

L

L

T

S

R

V

P

L

L

R

K

I

L

A

V

W

V

D

D

A

A

G

G

N

N

G

G

A

V

A

A

G

G

P

P

A

L

I

A

E

T

A

R

L

L

V

F

A

K

R

A

L

L

P

G

G

C

E

E

H

L

V

V

L

E

L

L

H

F

T

T

I

D

V

I

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

A

V

H

E

P

A

E

N

N

L

L

S

Q

D

D

L

V

R

I

E

A

A

K

V

L

A

K

R

A

G

T

S

D

L

A

D

E

F

I

G

G

L

F

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

L

G

G

A

V

I

V

D

T

S

K

T

D

G

G

R

Q

V

I

W

Y

G

P

D

D

Q

R

L

Q

L

L

A

M

I

L

F

F

A

A

E

E

D

E

L

V

L

L

R

S

N

R

R

N

P

P

G

G

A

A

T

Q

V

I

I

I

A

Y

D

D

V

V

K

K

A

C

S

T

S

R

A

N

L

L

F

A

H

R

R

W

I

V

A

R

E

E

L

K

G

G

R

E

P

P

E

L

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

V

K

K

S

A

R

K

M

L

K

R

Q

E

T

T

G

G

S

A

P

P

L

L

A

A

G

G

E

E

M

M

T

S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

D

P

R

Y

W

Y

Y

G

G

F

F

D

D

A

G

I

L

Y

Y

A

T

A

G

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

A

R

R

L

V

G

A

R

D

S

P

L

-

T

S

E

A

L

L

R

L

D

N

A

G

M

L

P

P

E

N

M

A

K

V

N

S

T

T

P

P

E

E

L

L

R

Q

F

L

A

-

P

-

G

-

R

-

R

N

L

V

A

K

V

L

V

I

E

D

E

K

V

L

L

R

G

A

R

D

L

A

R

K

A

F

A

D

G

G

A

A

-

D

R

E

V

V

T

V

D

T

I

I

D

D

G

G

A

L

R

R

V

V

D

E

T

Y

P

P

D

D

G

G

W

F

W

G

L

L

L

A

R

R

A

S

S

S

N

N

T

T

Q

T

D

P

M

V

L

V

V

V

V

L

R

R

A

F

E

E

S

A

R

T

S

S

E

D

E

A

G

A

L

L

A

A

R

R

L

I

active site: R13 (= R16), S109 (= S105), H110 (= H106), K119 (= K115), D243 (= D244), D245 (= D246), D247 (= D248), R248 (= R249), H330 (= H331)
binding zinc ion: D243 (= D244), D245 (= D246), D247 (= D248)

3rsmA Crystal structure of s108c mutant of pmm/pgm (see paper)
42% identity, 93% coverage: 23:456/467 of query aligns to 6:427/436 of 3rsmA

query
sites
3rsmA

L

L

E

T

V

A

E

E

D

T

A

A

R

Y

A

W

V

I

G

G

R

R

S

A

F

I

G

G

T

S

M

E

V

S

A

L

R

A

R

R

G

G

G

E

H

P

R

C

V

V

V

A

V

V

G

G

R

R

D

D

G

G

R

R

T

L

S

S

S

G

P

P

L

E

L

L

E

V

H

K

A

Q

L

L

V

I

E

Q

G

G

L

L

V

V

A

D

S

C

G

G

I

C

D

Q

V

V

V

S

R

D

I

V

G

G

V

M

G

V

P

P

T

T

P

P

M

V

L

L

Y

Y

A

A

E

-

A

A

S

N

M

V

P

L

E

E

V

G

Q

K

G

S

G

G

V

V

Q

M

V

L

T

T

G

G

S
x
C

H

-

N

-

P

-

A

-

D

-

H

-

N

N

G

G

F

F

K
|
K

L

I

V

V

F

V

E

A

G

G

S

E

A

T

L

L

F

A

G

N

T

E

D

Q

I

I

F

Q

D

A

L

L

G

R

R

E

L

R

A

I

G

E

E

K

G

N

D

D

W

L

I

A

S

S

G

G

S

V

G

G

R

S

V

V

E

E

E

Q

A

V

D

D

V

I

L

L

P

P

G

R

Y

Y

V

F

E

K

R

Q

L

I

L

R

Q

D

G

-

L

-

D

-

G

-

V

-

D

D

R

I

A

A

A

M

L

A

S

K

V

P

L

M

R

K

I

V

A

V

W

V

D

D

A

C

G

G

N

N

G

G

A

V

A

A

G

G

P

V

A

I

I

A

E

P

A

Q

L

L

V

I

A

E

R

A

L

L

P

G

G

C

E

S

H

V

V

I

L

P

L

L

H

Y

T

C

I

E

V

V

D

D

G

G

H

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

T

G

V

K

E

P

A

E

N

N

L

L

S

K

D

D

L

L

R

I

E

A

A

K

V

V

A

K

R

A

G

E

S

N

L

A

D

D

F

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

I

V

G

G

A

V

I

V

D

T

S

N

T

T

G

G

R

T

V

I

W

Y

G

P

D

D

Q

R

L

L

A

M

I

L

F

F

A

A

E

K

D

D

L

V

R

S

N

R

R

N

P

P

G

G

A

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

A

C

S

T

S

R

A

R

L

L

F

I

H

A

R

L

I

I

A

S

E

G

L

Y

G

G

R

R

P

P

E

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

S

K

R

K

M

M

K

K

Q

E

T

T

G

G

S

A

P

L

L

L

A

A

G

G

E

E

M

M

T

S

G

G

H
|
H

I

V

C

F

F

F

G

K

D

E

P

R

Y

W

Y

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

A

S

A

A

V

A

Q

R

L

L

M

L

A

E

A

I

T

L

V

S

R

Q

L

D

G

Q

R

R

S

D

L

S

T

E

E

H

L

V

R

F

D

S

A

A

M

F

P

P

E

S

M

D

K

I

N

S

T

T

P

P

E

E

L

I

R

N

F

I

A

T

A

V

P

T

P

E

G

D

R

S

R

K

L

F

A

A

V

I

E

E

E

A

V

L

L

Q

G

R

R

D

L

A

R

Q

A

W

A

G

G

E

A

G

R

N

V

I

T

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

D

D

T

Y

P

P

D

K

G

G

W

W

W

G

L

L

L

V

R

R

A

A

S

S

N

N

T

T

Q

T

D

P

M

V

L

L

V

V

V

L

R

R

A

F

E

E

S

A

R

D

S

T

E

E

G

E

L

L

A

E

R

R

L

I

L

K

A

T

Q

V

V

F

D

R

A

N

Q

Q

L

L

A

K

A

A

active site: C87 (≠ S105), K91 (= K115), D215 (= D244), D217 (= D246), D219 (= D248), R220 (= R249), H302 (= H331), D313 (= D342)
binding phosphate ion: C87 (≠ S105), D215 (= D244), D217 (= D246), D219 (= D248), R220 (= R249)
binding zinc ion: D215 (= D244), D217 (= D246), D219 (= D248)

1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
31% identity, 93% coverage: 13:447/467 of query aligns to 8:443/455 of 1wqaA

query
sites
1wqaA

Y

F

D

G

I

V

R
|
R

G

G

V

I

F

A

G

N

E

E

T

K

L

I

E

T

V

P

E

E

D

F

A

A

R

M

A

K

V

I

G

G

R

M

S

A

F

F

G

G

T

T

M

L

V

L

A

K

R

R

R

E

G

G

G

R

H

K

R

K

-

P

-

L

V

V

G

G

R

R

D

D

G

T

R

R

T

V

S

S

S

G

P

E

L

M

L

L

E

K

H

E

A

A

L

L

V

I

E

S

G

G

L

L

V

L

A

S

S

V

G

G

I

C

D

D

V

V

V

I

R

D

I

V

G

G

V

I

G

A

P

P

T

T

P

P

M

A

L

V

Y

Q

Y

W

A

A

E

T

A

K

S

H

M

F

P

-

E

N

V

A

Q

D

G

G

V

A

Q

V

V

I

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

P

D

E

H

Y

N

N

G

G

F

I

K
|
K

L

L

V

L

F

E

E

P

G

N

-

G

-

M

-

G

-

L

-

K

-

E

-

R

-

E

-

A

-

I

-

V

S

E

A

E

L

L

F

F

G

F

T

K

D

E

I

D

F

F

D

D

L

R

G

A

R

K

L

-

A

-

G

-

E

-

G

-

D

-

W

W

I

Y

S

E

G

-

S

I

G

G

R

E

V

V

E

R

A

E

D

D

V

I

L

I

P

K

G

P

Y

Y

V

I

E

E

R

A

L

I

L

K

Q

S

G

K

L

V

D

D

G

-

V

V

D

E

R

A

A

I

A

K

L

K

S

R

V

K

L

P

R

F

I

V

A

V

W

V

D

D

A

T

G

S

N

N

G

G

A

A

A

G

G

S

P

L

A

T

I

L

E

P

A

Y

L

L

V

L

A

R

R

E

L

L

P

G

G

C

E

K

H

V

V

I

L

T

L

V

H

N

T

A

I

Q

V

P

D

D

G

G

H

Y

F

F

P

P

N

A

H

R

H

N

P

P

D

E

P

P

T

N

V

-

E

E

A

E

N

N

L

L

S

K

D

E

L

F

R

M

E

E

A

I

V

V

A

K

R

A

G

L

S

G

L

A

D

D

F

F

G

G

L

V

A

A

F

Q

D
|
D

G

G

D
|
D

G

A

D
|
D

R
|
R

I

A

G

V

A

F

I

I

D

D

S

E

T

N

G

G

R

R

V

F

V

I

W

Q

G

G

D

D

Q

K

L

T

L

F

A

A

I

L

F

V

A

A

E

D

D

A

L

V

L

L

R

K

N

E

R

K

P

G

G

G

A

G

T

L

V

L

I

V

A

T

D

T

V

V

K

-

A

A

S

T

S

S

A

N

L

L

F

L

H

D

R

D

I

I

A

A

E

K

L

K

G

H

G

G

R

A

P

K

E

V

M

M

-

R

W

T

K

K

T

V

G

G

H

D

S

L

L

I

I

V

K

A

S

R

R

A

M

L

K

Y

Q

E

T

N

G

N

S

G

P

T

L

I

A

G

G

G

E

E

M

E

T

N

G

G

H
x
G

I

V

C

I

F

F

G

P

D

E

P

H

Y

V

Y

L

G

G

F
x
R

D

D

D

G

A

A

I

M

Y

T

A

V

A

A

V

-

Q

K

L

V

M

V

A

E

A

I

T

F

V

A

R

K

L

S

G

G

R

K

S

K

L

F

T

S

E

E

L

L

R

I

D

D

A

E

M

L

P

P

E

K

M

Y

K

Y

N

Q

T

I

P

K

E

T

L

K

R

R

F

H

A

V

A

E

P

-

G

G

R

D

R

R

L

H

A

A

V

I

V

V

E

N

E

K

V

V

L

A

G

E

R

M

L

A

R

R

A

E

A

R

G

G

A

Y

R

T

V

V

T

D

D

T

I

T

D

D

G

G

A

A

R

K

V

I

D

I

T

F

P

E

D

D

G

G

W

W

W

V

L

L

L

V

R

R

A

A

S

S

N

G

T

T

Q

E

D

P

M

I

L

I

V

R

V

I

R

F

A

S

E

E

S

A

R

K

S

S

E

K

E

E

G

K

L

A

A

Q

R

E

L

Y

L

L

active site: R11 (= R16), S101 (= S105), H102 (= H106), K111 (= K115), D243 (= D244), D245 (= D246), D247 (= D248), R248 (= R249), G330 (≠ H331), R340 (≠ F341)
binding magnesium ion: S101 (= S105), D243 (= D244), D245 (= D246), D247 (= D248)

6mlwA Crystal structure of x. Citri phosphoglucomutase in complex with 2- fluoro mannosyl-1-methyl-phosphonic acid (see paper)
32% identity, 97% coverage: 3:454/467 of query aligns to 1:446/449 of 6mlwA

query
sites
6mlwA

H

H

V

M

F

T

H

L

P

P

S

A

I

-

L

F

R

K

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

R

F

V

G

P

E

D

T

E

L

L

E

N

V

E

E

D

D

L

A

A

R

R

A

R

V

I

G

G

R

V

S

A

F

-

G

-

T

-

M

L

V

A

A

A

R

Q

R

L

G

D

G

Q

H

G

R

P

V

V

V

L

G

G

R

H

D

D

G

V

R

R

T

L

S

A

S

S

P

P

L

A

L

L

E

Q

H

E

A

A

L

L

V

S

E

A

G

G

L

L

V

R

A

A

S

S

G

G

I

R

D

D

V

V

V

I

R

D

I

I

G

G

V

L

G

C

P

G

T

T

P

E

M

E

L

V

Y

Y

Y

F

A

-

E

Q

A

T

S

D

M

Y

P

L

E

K

V

A

Q

A

G

G

V

V

Q

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

M

D

D

H

Y

N

N

G

G

F

M

K
|
K

L

L

V

V

F

R

E

E

G

Q

S

A

A

R

L

P

F

I

G

S

T

S

D

D

-

T

-

G

I

L

F

F

D

A

L

I

G

R

R

D

L

T

A

V

G

A

E

A

G

D

D

T

W

A

I

A

S

P

G

G

S

E

G

P

R

T

V

A

E

S

E

E

A

Q

D

S

V

R

L

T

-

D

-

K

P

T

G

A

Y

Y

V

L

E

E

R

H

L

L

Q

S

G

Y

L

-

D

-

G

-

V

V

D

D

R

R

A

S

A

T

L

L

S

K

V

P

L

L

R

K

I

L

A

V

W

V

D

N

A

A

G

G

N

N

G

G

A

G

A

A

G

G

P

L

A

I

I

V

E

D

A

L

L

L

V

A

A

P

R

H

L

L

P

P

G

F

E

E

H

F

V

V

L

R

L

V

H

F

T

H

I

E

V

P

D

D

G

G

H

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

T

L

V

L

E

P

A

E

N

N

L

R

S

D

D

A

L

T

R

A

E

K

A

A

V

V

A

K

R

D

G

N

S

G

L

A

D

D

F

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

I

C

G

F

A

F

I

F

D

D

S

H

T

T

G

G

R

R

V

F

V

I

W

E

G

G

D

Y

Q

Y

L

L

L

V

A

G

I

L

F

L

A

A

E

Q

D

A

L

I

L

L

R

A

N

K

R

Q

P

P

G

G

A

G

T

K

V

V

I

V

A

H

D

D

V

P

K

R

A

L

S

T

S

W

A

N

L

T

F

V

H

E

R

Q

I

V

A

E

E

E

L

A

G

G

R

I

P

P

E

V

M

L

W

C

K

K

T

S

G
|
G

H
|
H

S

A

L

F

I

I

K

K

S

E

R

K

M

M

K

R

Q

S

T

E

G

N

S

A

P

V

L

Y

A

G

G

G

E
|
E

M

M

T
x
S

G

A

H
|
H

I

H

C

Y

F

F

G

R

D

E

P

F

Y

A

Y

Y

G

A

F

-

D

D

D

S

A

G

I

M

Y

I

A

P

A

W

V

L

Q

L

L

I

M

A

A

E

A

L

T

V

V

S

R

Q

L

S

G

G

R

R

S

S

L

L

T

A

E

D

L

L

R

V

D

E

A

A

-

R

M

M

P

Q

E

K

M

F

K

P

N

C

T

S

P

G

E

E

L

I

R

N

F

F

A

K

A

V

P

A

P

D

G

A

R

K

R

-

L

-

A

A

V

S

V

V

E

A

E

R

V

V

L

M

G

E

R

H

L

Y

R

A

A

S

A

L

G

S

A

P

R

E

V

L

T

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

D

D

T

F

P

G

D

Q

G

W

W

R

W

F

L

N

L

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

D

P

M

L

L

L

V
x
R

V

L

R

N

A

V

E

E

S

T

R

R

S

G

E

D

E

A

G

A

L

L

A

L

R

E

L

T

L

R

A

T

Q

Q

V

E

D

I

A

S

Q

N

L

L

active site: R13 (= R16), S98 (= S105), H99 (= H106), K108 (= K115), D238 (= D244), D240 (= D246), D242 (= D248), R243 (= R249), H325 (= H331)
binding 2,6-anhydro-5,7-dideoxy-5-fluoro-7-phosphono-D-glycero-D-manno-heptitol: G303 (= G309), H304 (= H310), E321 (= E327), S323 (≠ T329), H325 (= H331), R415 (= R423), S417 (= S425), N418 (= N426), T419 (= T427), R424 (≠ V432)
binding magnesium ion: S98 (= S105), D238 (= D244), D240 (= D246), D242 (= D248)

5bmpA Crystal structure of phosphoglucomutase from xanthomonas citri complexed with glucose-1-phosphate (see paper)
32% identity, 97% coverage: 3:454/467 of query aligns to 1:446/449 of 5bmpA

query
sites
5bmpA

H

H

V

M

F

T

H

L

P

P

S

A

I

-

L

F

R

K

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

R

F

V

G

P

E

D

T

E

L

L

E

N

V

E

E

D

D

L

A

A

R

R

A

R

V

I

G

G

R

V

S

A

F

-

G

-

T

-

M

L

V

A

A

A

R

Q

R

L

G

D

G

Q

H

G

R

P

V

V

V

L

G

G

R

H

D

D

G

V

R

R

T

L

S

A

S

S

P

P

L

A

L

L

E

Q

H

E

A

A

L

L

V

S

E

A

G

G

L

L

V

R

A

A

S

S

G

G

I

R

D

D

V

V

V

I

R

D

I

I

G

G

V

L

G

C

P

G

T

T

P

E

M

E

L

V

Y

Y

Y

F

A

-

E

Q

A

T

S

D

M

Y

P

L

E

K

V

A

Q

A

G

G

V

V

Q

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

M

D

D

H

Y

N

N

G

G

F

M

K
|
K

L

L

V

V

F

R

E

E

G

Q

S

A

A

R

L

P

F

I

G

S

T

S

D

D

-

T

-

G

I

L

F

F

D

A

L

I

G

R

R

D

L

T

A

V

G

A

E

A

G

D

D

T

W

A

I

A

S

P

G

G

S

E

G

P

R

T

V

A

E

S

E

E

A

Q

D

S

V

R

L

T

-

D

-

K

P

T

G

A

Y

Y

V

L

E

E

R

H

L

L

Q

S

G

Y

L

-

D

-

G

-

V

V

D

D

R

R

A

S

A

T

L

L

S

K

V

P

L

L

R

K

I

L

A

V

W

V

D

N

A

A

G

G

N

N

G

G

A

G

A

A

G

G

P

L

A

I

I

V

E

D

A

L

L

L

V

A

A

P

R

H

L

L

P

P

G

F

E

E

H

F

V

V

L

R

L

V

H

F

T

H

I

E

V

P

D

D

G

G

H

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

T

L

V

L

E

P

A

E

N

N

L

R

S

D

D

A

L

T

R

A

E

K

A

A

V

V

A

K

R

D

G

N

S

G

L

A

D

D

F

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

I

C

G

F

A

F

I

F

D

D

S

H

T

T

G

G

R

R

V

F

V

I

W

E

G

G

D

Y

Q

Y

L

L

L

V

A

G

I

L

F

L

A

A

E

Q

D

A

L

I

L

L

R

A

N

K

R

Q

P

P

G

G

A

G

T

K

V

V

I

V

A

H

D

D

V

P

K
x
R

A

L

S

T

S

W

A

N

L

T

F

V

H

E

R

Q

I

V

A

E

E

E

L

A

G

G

R

I

P

P

E

V

M

L

W

C

K

K

T

S

G
|
G

H

H

S

A

L

F

I

I

K

K

S

E

R

K

M

M

K

R

Q

S

T

E

G

N

S

A

P

V

L

Y

A

G

G

G

E
|
E

M

M

T
x
S

G

A

H
|
H

I

H

C

Y

F

F

G

R

D

E

P

F

Y

A

Y

Y

G

A

F

-

D

D

D

S

A

G

I

M

Y

I

A

P

A

W

V

L

Q

L

L

I

M

A

A

E

A

L

T

V

V

S

R

Q

L

S

G

G

R

R

S

S

L

L

T

A

E

D

L

L

R

V

D

E

A

A

-

R

M

M

P

Q

E

K

M

F

K

P

N

C

T

S

P

G

E

E

L

I

R

N

F

F

A

K

A

V

P

A

P

D

G

A

R

K

R

-

L

-

A

A

V

S

V

V

E

A

E

R

V

V

L

M

G

E

R

H

L

Y

R

A

A

S

A

L

G

S

A

P

R

E

V

L

T

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

D

D

T

F

P

G

D

Q

G

W

W

R

W

F

L

N

L

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

D

P

M

L

L

L

V
x
R

V

L

R

N

A

V

E

E

S

T

R

R

S

G

E

D

E

A

G

A

L

L

A

L

R

E

L

T

L

R

A

T

Q

Q

V

E

D

I

A

S

Q

N

L

L

active site: R13 (= R16), S98 (= S105), H99 (= H106), K108 (= K115), D238 (= D244), D240 (= D246), D242 (= D248), R243 (= R249), H325 (= H331)
binding 1-O-phosphono-alpha-D-glucopyranose: R281 (≠ K287), G303 (= G309), E321 (= E327), S323 (≠ T329), H325 (= H331), R415 (= R423), S417 (= S425), N418 (= N426), T419 (= T427), R424 (≠ V432)
binding magnesium ion: S98 (= S105), D238 (= D244), D240 (= D246), D242 (= D248)

6nqhA Xanthomonas citri dephospho-pgm in complex with xylose-1-phosphate
32% identity, 95% coverage: 10:454/467 of query aligns to 6:445/448 of 6nqhA

query
sites
6nqhA

L

F

R

K

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

R

F

V

G

P

E

D

T

E

L

L

E

N

V

E

E

D

D

L

A

A

R

R

A

R

V

I

G

G

R

V

S

A

F

-

G

-

T

-

M

L

V

A

A

A

R

Q

R

L

G

D

G

Q

H

G

R

P

V

V

V

L

G

G

R

H

D

D

G

V

R

R

T

L

S

A

S

S

P

P

L

A

L

L

E

Q

H

E

A

A

L

L

V

S

E

A

G

G

L

L

V

R

A

A

S

S

G

G

I

R

D

D

V

V

V

I

R

D

I

I

G

G

V

L

G

C

P

G

T

T

P

E

M

E

L

V

Y

Y

Y

F

A

-

E

Q

A

T

S

D

M

Y

P

L

E

K

V

A

Q

A

G

G

V

V

Q

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

M

D

D

H

Y

N

N

G

G

F

M

K
|
K

L

L

V

V

F

R

E

E

G

Q

S

A

A

R

L

P

F

I

G

S

T

S

D

D

-

T

-

G

I

L

F

F

D

A

L

I

G

R

R

D

L

T

A

V

G

A

E

A

G

D

D

T

W

A

I

A

S

P

G

G

S

E

G

P

R

T

V

A

E

S

E

E

A

Q

D

S

V

R

L

T

-

D

-

K

P

T

G

A

Y

Y

V

L

E

E

R

H

L

L

Q

S

G

Y

L

-

D

-

G

-

V

V

D

D

R

R

A

S

A

T

L

L

S

K

V

P

L

L

R

K

I

L

A

V

W

V

D

N

A

A

G

G

N

N

G

G

A

G

A

A

G

G

P

L

A

I

I

V

E

D

A

L

L

L

V

A

A

P

R

H

L

L

P

P

G

F

E

E

H

F

V

V

L

R

L

V

H

F

T

H

I

E

V

P

D

D

G

G

H

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

T

L

V

L

E

P

A

E

N

N

L

R

S

D

D

A

L

T

R

A

E

K

A

A

V

V

A

K

R

D

G

N

S

G

L

A

D

D

F

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

I

C

G

F

A

F

I

F

D

D

S

H

T

T

G

G

R

R

V

F

V

I

W

E

G

G

D

Y

Q

Y

L

L

L

V

A

G

I

L

F

L

A

A

E

Q

D

A

L

I

L

L

R

A

N

K

R

Q

P

P

G

G

A

G

T

K

V

V

I

V

A

H

D

D

V

P

K
x
R

A

L

S

T

S

W

A

N

L

T

F

V

H

E

R

Q

I

V

A

E

E

E

L

A

G

G

R

I

P

P

E

V

M

L

W

C

K

K

T
x
S

G
|
G

H

H

S

A

L

F

I

I

K

K

S

E

R

K

M

M

K

R

Q

S

T

E

G

N

S

A

P

V

L

Y

A

G

G

G

E
|
E

M

M

T
x
S

G

A

H
|
H

I

H

C

Y

F

F

G

R

D

E

P

F

Y

A

Y

Y

G

A

F

-

D

D

D

S

A

G

I

M

Y

I

A

P

A

W

V

L

Q

L

L

I

M

A

A

E

A

L

T

V

V

S

R

Q

L

S

G

G

R

R

S

S

L

L

T

A

E

D

L

L

R

V

D

E

A

A

-

R

M

M

P

Q

E

K

M

F

K

P

N

C

T

S

P

G

E

E

L

I

R

N

F

F

A

K

A

V

P

A

P

D

G

A

R

K

R

-

L

-

A

A

V

S

V

V

E

A

E

R

V

V

L

M

G

E

R

H

L

Y

R

A

A

S

A

L

G

S

A

P

R

E

V

L

T

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

D

D

T

F

P

G

D

Q

G

W

W

R

W

F

L

N

L

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

D

P

M

L

L

L

V
x
R

V

L

R

N

A

V

E

E

S

T

R

R

S

G

E

D

E

A

G

A

L

L

A

L

R

E

L

T

L

R

A

T

Q

Q

V

E

D

I

A

S

Q

N

L

L

active site: R12 (= R16), S97 (= S105), H98 (= H106), K107 (= K115), D237 (= D244), D239 (= D246), D241 (= D248), R242 (= R249), H324 (= H331)
binding magnesium ion: D237 (= D244), D239 (= D246), D241 (= D248)
binding 1-O-phosphono-alpha-D-xylopyranose: R12 (= R16), S97 (= S105), H98 (= H106), K107 (= K115), D239 (= D246), R242 (= R249), R280 (≠ K287), S301 (≠ T308), G302 (= G309), E320 (= E327), S322 (≠ T329), H324 (= H331), R414 (= R423), S416 (= S425), N417 (= N426), T418 (= T427), R423 (≠ V432)

6np8A Xanthomonas citri phospho-pgm in complex with mannose-6-phosphate (see paper)
32% identity, 95% coverage: 10:454/467 of query aligns to 6:445/448 of 6np8A

query
sites
6np8A

L

F

R

K

E

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

R

F

V

G

P

E

D

T

E

L

L

E

N

V

E

E

D

D

L

A

A

R

R

A

R

V

I

G

G

R

V

S

A

F

-

G

-

T

-

M

L

V

A

A

A

R

Q

R

L

G

D

G

Q

H

G

R

P

V

V

V

L

G

G

R

H

D

D

G

V

R

R

T

L

S

A

S

S

P

P

L

A

L

L

E

Q

H

E

A

A

L

L

V

S

E

A

G

G

L

L

V

R

A

A

S

S

G

G

I

R

D

D

V

V

V

I

R

D

I

I

G

G

V

L

G

C

P

G

T

T

P

E

M

E

L

V

Y

Y

Y

F

A

-

E

Q

A

T

S

D

M

Y

P

L

E

K

V

A

Q

A

G

G

V

V

Q

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

M

D

D

H

Y

N

N

G

G

F

M

K
|
K

L

L

V

V

F

R

E

E

G

Q

S

A

A

R

L

P

F

I

G

S

T

S

D

D

-

T

-

G

I

L

F

F

D

A

L

I

G

R

R

D

L

T

A

V

G

A

E

A

G

D

D

T

W

A

I

A

S

P

G

G

S

E

G

P

R

T

V

A

E

S

E

E

A

Q

D

S

V

R

L

T

-

D

-

K

P

T

G

A

Y

Y

V

L

E

E

R

H

L

L

Q

S

G

Y

L

-

D

-

G

-

V

V

D

D

R

R

A

S

A

T

L

L

S

K

V

P

L

L

R

K

I

L

A

V

W

V

D

N

A

A

G

G

N

N

G

G

A

G

A

A

G

G

P

L

A

I

I

V

E

D

A

L

L

L

V

A

A

P

R

H

L

L

P

P

G

F

E

E

H

F

V

V

L

R

L

V

H

F

T

H

I

E

V

P

D

D

G

G

H

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

T

L

V

L

E

P

A

E

N

N

L

R

S

D

D

A

L

T

R

A

E

K

A

A

V

V

A

K

R

D

G

N

S

G

L

A

D

D

F

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

I

C

G

F

A

F

I

F

D

D

S

H

T

T

G

G

R

R

V

F

V

I

W

E

G

G

D

Y

Q

Y

L

L

L

V

A

G

I

L

F

L

A

A

E

Q

D

A

L

I

L

L

R

A

N

K

R

Q

P

P

G

G

A

G

T

K

V

V

I

V

A

H

D

D

V

P

K
x
R

A

L

S

T

S

W

A

N

L

T

F

V

H

E

R

Q

I

V

A

E

E

E

L

A

G

G

R

I

P

P

E

V

M

L

W

C

K

K

T

S

G
|
G

H
|
H

S

A

L

F

I

I

K

K

S

E

R

K

M

M

K

R

Q

S

T

E

G

N

S

A

P

V

L

Y

A

G

G

G

E
|
E

M

M

T
x
S

G

A

H
|
H

I

H

C

Y

F

F

G

R

D

E

P

F

Y

A

Y

Y

G

A

F

-

D

D

D

S

A

G

I

M

Y

I

A

P

A

W

V

L

Q

L

L

I

M

A

A

E

A

L

T

V

V

S

R

Q

L

S

G

G

R

R

S

S

L

L

T

A

E

D

L

L

R

V

D

E

A

A

-

R

M

M

P

Q

E

K

M

F

K

P

N

C

T

S

P

G

E

E

L

I

R

N

F

F

A

K

A

V

P

A

P

D

G

A

R

K

R

-

L

-

A

A

V

S

V

V

E

A

E

R

V

V

L

M

G

E

R

H

L

Y

R

A

A

S

A

L

G

S

A

P

R

E

V

L

T

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

D

D

T

F

P

G

D

Q

G

W

W

R

W

F

L

N

L

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

D

P

M

L

L

L

V
x
R

V

L

R

N

A

V

E

E

S

T

R

R

S

G

E

D

E

A

G

A

L

L

A

L

R

E

L

T

L

R

A

T

Q

Q

V

E

D

I

A

S

Q

N

L

L

active site: R12 (= R16), S97 (= S105), H98 (= H106), K107 (= K115), D237 (= D244), D239 (= D246), D241 (= D248), R242 (= R249), H324 (= H331)
binding calcium ion: S97 (= S105), D237 (= D244), D239 (= D246), D241 (= D248)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y13), R280 (≠ K287), G302 (= G309), H303 (= H310), E320 (= E327), S322 (≠ T329), H324 (= H331), R414 (= R423), S416 (= S425), N417 (= N426), T418 (= T427), R423 (≠ V432)

6nolA Xanthomonas citri dephospho-pgm in complex with mannose-1-phosphate (see paper)
32% identity, 95% coverage: 10:454/467 of query aligns to 6:445/448 of 6nolA

query
sites
6nolA

L

F

R

K

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

R

F

V

G

P

E

D

T

E

L

L

E

N

V

E

E

D

D

L

A

A

R

R

A

R

V

I

G

G

R

V

S

A

F

-

G

-

T

-

M

L

V

A

A

A

R

Q

R

L

G

D

G

Q

H

G

R

P

V

V

V

L

G

G

R

H

D

D

G

V

R

R

T

L

S

A

S

S

P

P

L

A

L

L

E

Q

H

E

A

A

L

L

V

S

E

A

G

G

L

L

V

R

A

A

S

S

G

G

I

R

D

D

V

V

V

I

R

D

I

I

G

G

V

L

G

C

P

G

T

T

P

E

M

E

L

V

Y

Y

Y

F

A

-

E

Q

A

T

S

D

M

Y

P

L

E

K

V

A

Q

A

G

G

V

V

Q

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

M

D

D

H

Y

N

N

G

G

F

M

K
|
K

L

L

V

V

F

R

E

E

G

Q

S

A

A

R

L

P

F

I

G

S

T

S

D

D

-

T

-

G

I

L

F

F

D

A

L

I

G

R

R

D

L

T

A

V

G

A

E

A

G

D

D

T

W

A

I

A

S

P

G

G

S

E

G

P

R

T

V

A

E

S

E

E

A

Q

D

S

V

R

L

T

-

D

-

K

P

T

G

A

Y

Y

V

L

E

E

R

H

L

L

Q

S

G

Y

L

-

D

-

G

-

V

V

D

D

R

R

A

S

A

T

L

L

S

K

V

P

L

L

R

K

I

L

A

V

W

V

D

N

A

A

G

G

N

N

G

G

A

G

A

A

G

G

P

L

A

I

I

V

E

D

A

L

L

L

V

A

A

P

R

H

L

L

P

P

G

F

E

E

H

F

V

V

L

R

L

V

H

F

T

H

I

E

V

P

D

D

G

G

H

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

T

L

V

L

E

P

A

E

N

N

L

R

S

D

D

A

L

T

R

A

E

K

A

A

V

V

A

K

R

D

G

N

S

G

L

A

D

D

F

F

G

G

L

I

A

A

F

W

D
|
D

G

G

D
|
D

G

F

D
|
D

R
|
R

I

C

G

F

A

F

I

F

D

D

S

H

T

T

G

G

R

R

V

F

V

I

W

E

G

G

D

Y

Q

Y

L

L

L

V

A

G

I

L

F

L

A

A

E

Q

D

A

L

I

L

L

R

A

N

K

R

Q

P

P

G

G

A

G

T

K

V

V

I

V

A

H

D

D

V

P

K

R

A

L

S

T

S

W

A

N

L

T

F

V

H

E

R

Q

I

V

A

E

E

E

L

A

G

G

R

I

P

P

E

V

M

L

W

C

K

K

T

S

G
|
G

H

H

S

A

L

F

I

I

K

K

S

E

R

K

M

M

K

R

Q

S

T

E

G

N

S

A

P

V

L

Y

A

G

G

G

E
|
E

M

M

T
x
S

G

A

H
|
H

I

H

C

Y

F

F

G

R

D

E

P

F

Y

A

Y

Y

G

A

F

-

D

D

D

S

A

G

I

M

Y

I

A

P

A

W

V

L

Q

L

L

I

M

A

A

E

A

L

T

V

V

S

R

Q

L

S

G

G

R

R

S

S

L

L

T

A

E

D

L

L

R

V

D

E

A

A

-

R

M

M

P

Q

E

K

M

F

K

P

N

C

T

S

P

G

E

E

L

I

R

N

F

F

A

K

A

V

P

A

P

D

G

A

R

K

R

-

L

-

A

A

V

S

V

V

E

A

E

R

V

V

L

M

G

E

R

H

L

Y

R

A

A

S

A

L

G

S

A

P

R

E

V

L

T

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

D

D

T

F

P

G

D

Q

G

W

W

R

W

F

L

N

L

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

D

P

M

L

L

L

V
x
R

V

L

R

N

A

V

E

E

S

T

R

R

S

G

E

D

E

A

G

A

L

L

A

L

R

E

L

T

L

R

A

T

Q

Q

V

E

D

I

A

S

Q

N

L

L

active site: R12 (= R16), S97 (= S105), H98 (= H106), K107 (= K115), D237 (= D244), D239 (= D246), D241 (= D248), R242 (= R249), H324 (= H331)
binding 1-O-phosphono-alpha-D-mannopyranose: G302 (= G309), E320 (= E327), S322 (≠ T329), H324 (= H331), R414 (= R423), S416 (= S425), N417 (= N426), T418 (= T427), R423 (≠ V432)
binding magnesium ion: S97 (= S105), D237 (= D244), D239 (= D246), D241 (= D248)

6nnpA Xanthomonas citri dephospho-pgm in complex with glucose-6-phosphate (see paper)
32% identity, 95% coverage: 10:454/467 of query aligns to 6:445/448 of 6nnpA

query
sites
6nnpA

L

F

R

K

E

A

Y

Y

D

D

I

I

R
|
R

G

G

V

R

F

V

G

P

E

D

T

E

L

L

E

N

V

E

E

D

D

L

A

A

R

R

A

R

V

I

G

G

R

V

S

A

F

-

G

-

T

-

M

L

V

A

A

A

R

Q

R

L

G

D

G

Q

H

G

R

P

V

V

V

L

G

G

R

H

D

D

G

V

R

R

T

L

S

A

S

S

P

P

L

A

L

L

E

Q

H

E

A

A

L

L

V

S

E

A

G

G

L

L

V

R

A

A

S

S

G

G

I

R

D

D

V

V

V

I

R

D

I

I

G

G

V

L

G

C

P

G

T

T

P

E

M

E

L

V

Y

Y

Y

F

A

-

E

Q

A

T

S

D

M

Y

P

L

E

K

V

A

Q

A

G

G

V

V

Q

M

V

V

T

T

G

A

S
|
S

H
|
H

N

N

P

P

A

M

D

D

H

Y

N

N

G

G

F

M

K
|
K

L

L

V

V

F

R

E

E

G

Q

S

A

A

R

L

P

F

I

G

S

T

S

D

D

-

T

-

G

I

L

F

F

D

A

L

I

G

R

R

D

L

T

A

V

G

A

E

A

G

D

D

T

W

A

I

A

S

P

G

G

S

E

G

P

R

T

V

A

E

S

E

E

A

Q

D

S

V

R

L

T

-

D

-

K

P

T

G

A

Y

Y

V

L

E

E

R

H

L

L

Q

S

G

Y

L

-

D

-

G

-

V

V

D

D

R

R

A

S

A

T

L

L

S

K

V

P

L

L

R

K

I

L

A

V

W

V

D

N

A

A

G

G

N

N

G

G

A

G

A

A

G

G

P

L

A

I

I

V

E

D

A

L

L

L

V

A

A

P

R

H

L

L

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L

active site: R12 (= R16), S97 (= S105), H98 (= H106), K107 (= K115), D237 (= D244), D239 (= D246), D241 (= D248), R242 (= R249), H324 (= H331)
binding 6-O-phosphono-alpha-D-glucopyranose: R280 (≠ K287), G302 (= G309), H303 (= H310), E320 (= E327), H324 (= H331), R414 (= R423), S416 (= S425), N417 (= N426), T418 (= T427), R423 (≠ V432)
binding magnesium ion: S97 (= S105), D237 (= D244), D239 (= D246), D241 (= D248)

Query Sequence

>WP_011446953.1 NCBI__GCF_000013325.1:WP_011446953.1
MSHVFHPSILREYDIRGVFGETLEVEDARAVGRSFGTMVARRGGHRVVVGRDGRTSSPLL
EHALVEGLVASGIDVVRIGVGPTPMLYYAEASMPEVQGGVQVTGSHNPADHNGFKLVFEG
SALFGTDIFDLGRLAGEGDWISGSGRVEEADVLPGYVERLLQGLDGVDRAALSVLRIAWD
AGNGAAGPAIEALVARLPGEHVLLHTIVDGHFPNHHPDPTVEANLSDLREAVARGSLDFG
LAFDGDGDRIGAIDSTGRVVWGDQLLAIFAEDLLRNRPGATVIADVKASSALFHRIAELG
GRPEMWKTGHSLIKSRMKQTGSPLAGEMTGHICFGDPYYGFDDAIYAAVQLMAATVRLGR
SLTELRDAMPEMKNTPELRFAAPPGRRLAVVEEVLGRLRAAGARVTDIDGARVDTPDGWW
LLRASNTQDMLVVRAESRSEEGLARLLAQVDAQLAASGCVRGPEAAH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory