SitesBLAST
Comparing WP_011514206.1 NCBI__GCF_000013905.1:WP_011514206.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
25% identity, 70% coverage: 5:252/352 of query aligns to 55:305/466 of 6lpxA
- binding flavin-adenine dinucleotide: P75 (≠ I24), Q76 (≠ M25), G77 (= G26), G78 (= G27), N79 (≠ G28), T80 (≠ S29), G81 (≠ K30), M82 (≠ Q31), G85 (= G34), S86 (≠ R35), L139 (≠ P86), G140 (≠ P87), A141 (≠ T88), C145 (≠ A93), G149 (= G97), N150 (≠ T98), A152 (= A100), T153 (≠ C101), G157 (= G105), G207 (= G155), I212 (= I160)
Sites not aligning to the query:
- binding 2-oxoglutaric acid: 333, 337, 348, 379, 381, 388, 423
- binding flavin-adenine dinucleotide: 39, 422, 459
- binding zinc ion: 381, 388, 422
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
25% identity, 70% coverage: 5:252/352 of query aligns to 55:305/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (≠ I24), G77 (= G26), G78 (= G27), N79 (≠ G28), T80 (≠ S29), G81 (≠ K30), G85 (= G34), S86 (≠ R35), L139 (≠ P86), G140 (≠ P87), A141 (≠ T88), C145 (≠ A93), H146 (≠ T94), G148 (= G96), G149 (= G97), N150 (≠ T98), A152 (= A100), T153 (≠ C101), A155 (≠ L103), E206 (≠ M154), G207 (= G155), I211 (= I159), I212 (= I160)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 39, 422, 459
- binding (2s)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 423
- binding zinc ion: 381, 388, 422
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
25% identity, 70% coverage: 5:252/352 of query aligns to 55:305/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (≠ I24), G77 (= G26), G78 (= G27), N79 (≠ G28), T80 (≠ S29), G81 (≠ K30), G85 (= G34), S86 (≠ R35), L139 (≠ P86), G140 (≠ P87), A141 (≠ T88), C145 (≠ A93), H146 (≠ T94), G149 (= G97), N150 (≠ T98), A152 (= A100), T153 (≠ C101), A155 (≠ L103), G157 (= G105), E206 (≠ M154), G207 (= G155), I211 (= I159), I212 (= I160)
- binding d-malate: M82 (≠ Q31)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 39, 422, 459
- binding d-malate: 333, 337, 348, 379, 381, 388, 422, 423
- binding zinc ion: 381, 388, 422
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
25% identity, 70% coverage: 5:252/352 of query aligns to 55:305/466 of 6lppA
- binding flavin-adenine dinucleotide: P75 (≠ I24), G77 (= G26), G78 (= G27), N79 (≠ G28), T80 (≠ S29), G81 (≠ K30), M82 (≠ Q31), G85 (= G34), S86 (≠ R35), L139 (≠ P86), G140 (≠ P87), A141 (≠ T88), C145 (≠ A93), G149 (= G97), N150 (≠ T98), A152 (= A100), T153 (≠ C101), A155 (≠ L103), G157 (= G105), G207 (= G155), I212 (= I160)
Sites not aligning to the query:
- binding (2R)-2-hydroxypentanedioic acid: 333, 337, 348, 379, 381, 388, 390, 423
- binding flavin-adenine dinucleotide: 39, 422, 423
- binding zinc ion: 381, 388, 422
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
25% identity, 70% coverage: 5:252/352 of query aligns to 56:306/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (≠ I24), G78 (= G26), G79 (= G27), N80 (≠ G28), T81 (≠ S29), G82 (≠ K30), M83 (≠ Q31), G86 (= G34), S87 (≠ R35), L140 (≠ P86), A142 (≠ T88), C146 (≠ A93), H147 (≠ T94), G150 (= G97), N151 (≠ T98), A153 (= A100), T154 (≠ C101), G208 (= G155), I212 (= I159), I213 (= I160)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
25% identity, 70% coverage: 5:252/352 of query aligns to 108:358/521 of Q8N465
- S109 (≠ Q6) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ Q23) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G27) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ S43) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (vs. gap) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ T66) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ A83) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A100) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ G126) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (≠ A128) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 375 D → Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- 386 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; R→A: Loss of catalytic activity.
- 390 binding (R)-2-hydroxyglutarate; binding (R)-malate; T→A: Significantly reduced catalytic activity.
- 399 V → M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- 401 binding (R)-2-hydroxyglutarate; binding (R)-malate; K→A: Loss of catalytic activity.
- 419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- 426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- 434 binding Zn(2+); H→A: Loss of catalytic activity.
- 436 G → V: slight reduction in catalytic activity
- 439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- 441 binding Zn(2+); H→A: Loss of catalytic activity.
- 443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
- 444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- 446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- 475 binding Zn(2+); E→A: Loss of catalytic activity.
- 476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
- 477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
24% identity, 49% coverage: 5:177/352 of query aligns to 53:226/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ I24), G75 (= G26), S76 (≠ G27), G77 (= G28), T78 (≠ S29), G79 (≠ K30), L80 (≠ Q31), A83 (≠ G34), C84 (≠ R35), P137 (≠ T88), G138 (≠ F89), E139 (≠ D90), A142 (= A93), T143 (= T94), G146 (= G97), N147 (≠ T98), S149 (≠ A100), T150 (≠ C101), A152 (≠ L103), G153 (≠ S104), E203 (≠ M154), G204 (= G155), I209 (= I160)
Sites not aligning to the query:
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 48% coverage: 5:173/352 of query aligns to 76:246/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
29% identity, 49% coverage: 5:176/352 of query aligns to 50:222/465 of 3pm9A
- active site: A149 (≠ L103), L159 (≠ S113)
- binding flavin-adenine dinucleotide: P69 (≠ I24), Q70 (≠ M25), G71 (= G26), G72 (= G27), N73 (≠ G28), T74 (≠ S29), G75 (≠ K30), L76 (≠ Q31), G79 (= G34), Q80 (≠ R35), L91 (= L44), L133 (≠ P86), G134 (≠ P87), A135 (≠ T88), C139 (≠ A93), T140 (= T94), G142 (= G96), G143 (= G97), S146 (≠ A100), T147 (≠ C101), A149 (≠ L103), G150 (≠ S104), E200 (≠ M154), G201 (= G155), I205 (= I159), I206 (= I160)
Sites not aligning to the query:
O96759 Alkyldihydroxyacetonephosphate synthase; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Dictyostelium discoideum (Social amoeba) (see paper)
27% identity, 57% coverage: 5:205/352 of query aligns to 149:365/611 of O96759
- 169:175 (vs. 25:30, 71% identical) binding FAD
- 237:243 (vs. 87:94, 25% identical) binding FAD
- TCSSGH 250:255 (≠ CHLSGP 101:106) binding FAD
- 301:307 (vs. 154:160, 86% identical) binding FAD
- R352 (= R192) mutation to H: 30-fold reduction in activity.
Sites not aligning to the query:
- 447 R→L: Loss of activity. The FAD binds poorly to the mutant enzyme.
- 508 active site, Proton donor/acceptor; Y→F: Loss of activity.
- 544 H→I: Loss of activity.
- 545 H→I: Loss of activity.
- 546 H→I: Loss of activity.
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
25% identity, 47% coverage: 5:170/352 of query aligns to 48:214/459 of P9WIT1
Sites not aligning to the query:
- 354 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
2uuuA Alkyldihydroxyacetonephosphate synthase in p212121 (see paper)
26% identity, 57% coverage: 5:205/352 of query aligns to 138:347/550 of 2uuuA
- binding flavin-adenine dinucleotide: P158 (vs. gap), G160 (= G26), G161 (= G27), G162 (= G28), S163 (= S29), N164 (≠ K30), I165 (≠ Q31), A168 (≠ G34), I169 (≠ R35), P225 (= P86), D226 (≠ P87), S227 (≠ T88), S231 (≠ A93), T232 (= T94), G235 (= G97), W236 (≠ T98), A238 (= A100), T239 (≠ C101), S241 (≠ L103), S242 (= S104), E283 (≠ M154), G284 (= G155), I288 (= I159), I289 (= I160)
- binding hexadecan-1-ol: I165 (≠ Q31)
Sites not aligning to the query:
O00116 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Homo sapiens (Human) (see 2 papers)
28% identity, 37% coverage: 42:171/352 of query aligns to 258:385/658 of O00116
Sites not aligning to the query:
- 182 R → Q: in RCDP3; severely reduced protein levels
- 419 Important for enzyme activity; R → H: in RCDP3; loss of enzyme activity; dbSNP:rs121434411
- 471 E → K: in RCDP3; severely reduced protein levels
- 568 T → M: in RCDP3; does not affect protein levels; dbSNP:rs387907214
5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
27% identity, 37% coverage: 42:171/352 of query aligns to 169:296/561 of 5ae2A
- binding flavin-adenine dinucleotide: P213 (= P86), D214 (≠ P87), S215 (≠ T88), S219 (≠ A93), T220 (= T94), G223 (= G97), W224 (≠ T98), S226 (≠ A100), T227 (≠ C101), A229 (≠ L103), S230 (= S104), E279 (≠ M154), V284 (≠ I159), I285 (= I160)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 16, 145, 146, 147, 148, 149, 150, 151, 155, 156, 519
- binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 415, 418, 428, 429, 430, 481, 483, 519
5ae3A Ether lipid-generating enzyme agps in complex with antimycin a (see paper)
27% identity, 37% coverage: 42:171/352 of query aligns to 164:291/539 of 5ae3A
- binding flavin-adenine dinucleotide: P208 (= P86), D209 (≠ P87), S210 (≠ T88), S214 (≠ A93), T215 (= T94), G218 (= G97), W219 (≠ T98), S221 (≠ A100), T222 (≠ C101), A224 (≠ L103), S225 (= S104), E274 (≠ M154), V279 (≠ I159), I280 (= I160)
Sites not aligning to the query:
- binding [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 3-methylbutanoate: 45, 49, 53, 325, 327, 331, 334, 349, 386, 393, 396, 400, 447, 461
- binding flavin-adenine dinucleotide: 140, 141, 142, 143, 144, 145, 146, 147, 151, 497
6gouA Development of alkyl glycerone phosphate synthase inhibitors: complex with inhibitor 2i (see paper)
27% identity, 37% coverage: 42:171/352 of query aligns to 178:305/554 of 6gouA
- binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: D223 (≠ P87)
- binding flavin-adenine dinucleotide: P222 (= P86), D223 (≠ P87), S224 (≠ T88), S228 (≠ A93), T229 (= T94), G232 (= G97), W233 (≠ T98), S235 (≠ A100), T236 (≠ C101), A238 (≠ L103), S239 (= S104), E288 (≠ M154), V293 (≠ I159), I294 (= I160)
Sites not aligning to the query:
- binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: 411, 421, 422, 423, 474, 476, 478
- binding flavin-adenine dinucleotide: 16, 154, 155, 156, 157, 158, 159, 160, 161, 164, 512
P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
27% identity, 37% coverage: 42:171/352 of query aligns to 258:385/658 of P97275
- H300 (≠ F84) mutation to A: Loss of activity.
- 303:309 (vs. 87:94, 13% identical) binding FAD
- T309 (= T94) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
- TRAS 316:319 (≠ CHLS 101:104) binding FAD
- S367 (≠ A153) mutation to A: Strongly reduced activity.
- 368:374 (vs. 154:160, 71% identical) binding FAD
Sites not aligning to the query:
- 1:58 modified: transit peptide, Peroxisome
- 234:240 binding FAD
- 419 Important for enzyme activity; R→H: Loss of activity.; R→K: Strongly reduced activity.
- 469 L→P: Impaired FAD binding and protein stability. Loss of activity.
- 515 binding substrate; R→L: Impaired FAD binding and protein stability. Loss of activity.
- 576 C→A: No effect on activity.
- 578 active site, Proton donor/acceptor; Y→F: Loss of activity.
- 615 H→A: Loss of activity.
- 615:617 Important for enzyme activity
- 616 H→A: Loss of activity.
- 617 H→A: Loss of activity.
- 654:658 Important for enzyme activity
5adzC Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
27% identity, 37% coverage: 42:171/352 of query aligns to 178:305/557 of 5adzC
- binding flavin-adenine dinucleotide: P222 (= P86), D223 (≠ P87), S224 (≠ T88), S228 (≠ A93), T229 (= T94), G232 (= G97), W233 (≠ T98), S235 (≠ A100), T236 (≠ C101), A238 (≠ L103), S239 (= S104), E288 (≠ M154), G289 (= G155), I294 (= I160)
- binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D223 (≠ P87)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 16, 154, 155, 156, 157, 158, 159, 160, 515
- binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): 410, 414, 424, 425, 426, 477, 515, 516
4bc9A Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
27% identity, 37% coverage: 42:171/352 of query aligns to 169:296/555 of 4bc9A
- binding propanenitrile: D214 (≠ P87)
- binding flavin-adenine dinucleotide: P213 (= P86), D214 (≠ P87), S215 (≠ T88), S219 (≠ A93), T220 (= T94), G223 (= G97), W224 (≠ T98), S226 (≠ A100), T227 (≠ C101), A229 (≠ L103), S230 (= S104), E279 (≠ M154), V284 (≠ I159), I285 (= I160)
Sites not aligning to the query:
- binding propanenitrile: 475, 513, 514
- binding flavin-adenine dinucleotide: 145, 146, 147, 148, 149, 150, 151, 152, 155, 156, 513
5ae1A Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
27% identity, 37% coverage: 42:171/352 of query aligns to 163:290/560 of 5ae1A
- binding flavin-adenine dinucleotide: P207 (= P86), D208 (≠ P87), S209 (≠ T88), S213 (≠ A93), T214 (= T94), G216 (= G96), G217 (= G97), W218 (≠ T98), S220 (≠ A100), T221 (≠ C101), A223 (≠ L103), S224 (= S104), E273 (≠ M154),