SitesBLAST
Comparing WP_011605777.1 NCBI__GCF_000058485.1:WP_011605777.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
32% identity, 84% coverage: 25:311/342 of query aligns to 14:294/301 of 6thqB
- active site: F37 (= F48), K156 (= K170), E190 (= E204), L214 (= L228)
- binding pyridoxal-5'-phosphate: R60 (= R74), K156 (= K170), Y161 (= Y175), E190 (= E204), N195 (≠ T209), L214 (= L228), G216 (= G230), I217 (= I231), T218 (≠ S232), T254 (= T268)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R74), Y97 (= Y110), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), E194 (≠ A208), N195 (≠ T209), G216 (= G230), I217 (= I231), T218 (≠ S232), G253 (= G267), T254 (= T268), A255 (≠ G269)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
31% identity, 84% coverage: 25:310/342 of query aligns to 14:299/306 of 4whxA
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
29% identity, 84% coverage: 20:305/342 of query aligns to 6:292/307 of 6q8eA
- active site: F34 (= F48), K156 (= K170), E190 (= E204), L214 (= L228)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R74), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), S194 (≠ A208), C195 (≠ T209), L214 (= L228), S216 (≠ G230), I217 (= I231), T218 (≠ S232), G254 (= G267), T255 (= T268)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
29% identity, 82% coverage: 20:300/342 of query aligns to 5:286/304 of 1iyeA
- active site: F33 (= F48), G35 (= G50), K156 (= K170), A157 (≠ N171), E190 (= E204), L214 (= L228)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R74), Y92 (= Y110), Y126 (≠ K141), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), E194 (≠ A208), N195 (≠ T209), L214 (= L228), G216 (= G230), I217 (= I231), T218 (≠ S232), G253 (= G267), T254 (= T268), A255 (≠ G269)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
29% identity, 82% coverage: 20:300/342 of query aligns to 5:286/304 of 1iydA
- active site: F33 (= F48), G35 (= G50), K156 (= K170), A157 (≠ N171), E190 (= E204), L214 (= L228)
- binding glutaric acid: Y92 (= Y110), Y126 (≠ K141), A255 (≠ G269)
- binding pyridoxal-5'-phosphate: R56 (= R74), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), E194 (≠ A208), L214 (= L228), G216 (= G230), I217 (= I231), T218 (≠ S232), T254 (= T268)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
29% identity, 82% coverage: 20:300/342 of query aligns to 5:286/304 of 1i1mA
- active site: K156 (= K170)
- binding 4-methyl valeric acid: Y92 (= Y110), K156 (= K170), T254 (= T268), A255 (≠ G269)
- binding pyridoxal-5'-phosphate: R56 (= R74), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), E194 (≠ A208), L214 (= L228), G216 (= G230), I217 (= I231), T218 (≠ S232), G253 (= G267), T254 (= T268)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
29% identity, 82% coverage: 20:300/342 of query aligns to 5:286/304 of 1i1lA
- active site: K156 (= K170)
- binding 2-methylleucine: Y92 (= Y110), K156 (= K170), T254 (= T268), A255 (≠ G269)
- binding pyridoxal-5'-phosphate: R56 (= R74), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), G216 (= G230), I217 (= I231), T218 (≠ S232), T254 (= T268)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
32% identity, 84% coverage: 19:305/342 of query aligns to 6:284/297 of 2ej3A
- active site: F35 (= F48), G37 (= G50), K150 (= K170), E184 (= E204), L207 (= L228)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (≠ T207), G246 (= G267), T247 (= T268), A248 (≠ G269)
- binding pyridoxal-5'-phosphate: R58 (= R74), K150 (= K170), Y155 (= Y175), E184 (= E204), G187 (≠ T207), L207 (= L228), G209 (= G230), I210 (= I231), T211 (≠ S232), G246 (= G267), T247 (= T268)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
32% identity, 84% coverage: 19:305/342 of query aligns to 6:284/297 of 2eiyA
- active site: F35 (= F48), G37 (= G50), K150 (= K170), E184 (= E204), L207 (= L228)
- binding 4-methyl valeric acid: F35 (= F48), Y94 (= Y110), T247 (= T268), A248 (≠ G269)
- binding pyridoxal-5'-phosphate: R58 (= R74), K150 (= K170), Y155 (= Y175), E184 (= E204), G187 (≠ T207), E188 (≠ A208), L207 (= L228), G209 (= G230), I210 (= I231), T211 (≠ S232), G246 (= G267), T247 (= T268)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
32% identity, 84% coverage: 19:305/342 of query aligns to 6:284/297 of 1wrvA
- active site: F35 (= F48), G37 (= G50), K150 (= K170), E184 (= E204), L207 (= L228)
- binding pyridoxal-5'-phosphate: R58 (= R74), K150 (= K170), Y155 (= Y175), E184 (= E204), G187 (≠ T207), L207 (= L228), G209 (= G230), I210 (= I231), T211 (≠ S232), T247 (= T268)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
27% identity, 84% coverage: 20:305/342 of query aligns to 6:292/309 of 7neaA
- active site: F34 (= F48), K156 (= K170), E190 (= E204), L214 (= L228)
- binding pyridoxal-5'-phosphate: R59 (= R74), K156 (= K170), Y161 (= Y175), E190 (= E204), G193 (≠ T207), S194 (≠ A208), L214 (= L228), S216 (≠ G230), I217 (= I231), T218 (≠ S232), T255 (= T268)
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
31% identity, 84% coverage: 19:305/342 of query aligns to 6:292/305 of 2ej0B
- active site: F35 (= F48), G37 (= G50), K158 (= K170), E192 (= E204), L215 (= L228)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R74), Y163 (= Y175), E192 (= E204), G195 (≠ T207), E196 (≠ A208), L215 (= L228), G217 (= G230), I218 (= I231), T219 (≠ S232), G254 (= G267), T255 (= T268)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
32% identity, 84% coverage: 19:305/342 of query aligns to 6:281/294 of 2ej2A
- active site: F35 (= F48), G37 (= G50), K147 (= K170), E181 (= E204), L204 (= L228)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R74), Y94 (= Y110), Y152 (= Y175), E181 (= E204), G184 (≠ T207), E185 (≠ A208), L204 (= L228), G206 (= G230), I207 (= I231), T208 (≠ S232), T244 (= T268), A245 (≠ G269)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
28% identity, 81% coverage: 25:300/342 of query aligns to 12:289/308 of 6h65C
- active site: F35 (= F48), K158 (= K170), E192 (= E204), L216 (= L228)
- binding pyridoxal-5'-phosphate: R60 (= R74), K158 (= K170), Y163 (= Y175), E192 (= E204), A196 (= A208), L216 (= L228), S218 (≠ G230), V219 (≠ I231), T220 (≠ S232), G256 (= G267), T257 (= T268)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
28% identity, 76% coverage: 20:280/342 of query aligns to 4:258/290 of 5mr0D
- active site: F32 (= F48), G34 (= G50), K150 (= K170), E183 (= E204), L206 (= L228)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R74), G100 (vs. gap), L101 (vs. gap), K150 (= K170), Y154 (= Y175), E183 (= E204), G186 (≠ T207), D187 (≠ A208), L206 (= L228), I209 (= I231), T210 (≠ S232), G245 (= G267), T246 (= T268)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
25% identity, 82% coverage: 19:299/342 of query aligns to 4:278/290 of 5e25A
- active site: F33 (= F48), G35 (= G50), K151 (= K170), E184 (= E204), L207 (= L228)
- binding 2-oxoglutaric acid: Y88 (= Y110), K151 (= K170), T247 (= T268), A248 (≠ G269)
- binding pyridoxal-5'-phosphate: R52 (= R74), K151 (= K170), Y155 (= Y175), E184 (= E204), G187 (≠ T207), D188 (≠ A208), L207 (= L228), G209 (= G230), I210 (= I231), T211 (≠ S232), G246 (= G267), T247 (= T268)
7z79B Crystal structure of aminotransferase-like protein from variovorax paradoxus
28% identity, 82% coverage: 19:299/342 of query aligns to 17:291/306 of 7z79B
- binding pyridoxal-5'-phosphate: W46 (≠ F48), R65 (= R74), N166 (≠ Y175), S202 (≠ T207), T203 (≠ A208), F222 (≠ L228), G224 (= G230), I225 (= I231), T226 (≠ S232), G261 (= G267), T262 (= T268)
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
28% identity, 76% coverage: 21:281/342 of query aligns to 50:327/368 of A0R066
- R101 (= R74) binding pyridoxal 5'-phosphate
- Y209 (= Y175) binding pyridoxal 5'-phosphate
- K299 (≠ Q259) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T268) binding pyridoxal 5'-phosphate
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
28% identity, 76% coverage: 21:281/342 of query aligns to 45:322/363 of 3dtgA
- active site: K199 (= K170)
- binding O-benzylhydroxylamine: F72 (= F48), Y171 (≠ W143), Y204 (= Y175)
- binding pyridoxal-5'-phosphate: R96 (= R74), R189 (≠ P160), K199 (= K170), Y204 (= Y175), E235 (= E204), G238 (≠ T207), L263 (= L228), I266 (= I231), T267 (≠ S232), T309 (= T268)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
28% identity, 76% coverage: 21:281/342 of query aligns to 45:322/363 of 3dtfA
Query Sequence
>WP_011605777.1 NCBI__GCF_000058485.1:WP_011605777.1
MGSEAGPAGAGGPAPAGPLVYHREQLVPAQAATLPVGSIALRYGISVFEGVRLYRQEAPG
LGLRPWLLDQHLDRLRNSCRLMLLDDTCCDDVPRIIDELVAVNDTQVDSYARIAVSAVDA
GGIGDGSEAALTVSVTPSGRKRWLRDGEGLRLTVSSWQRPSSAVFPSAAKNISAYAGPRL
ALAQAVAAGFDSCLLCTAEGLVSEAPTATVFLAEGTRLVTPRLGDAVLPGISRAWVLATA
PRLGLSVAEEAVTPDRIRQADEVFLCGTGIEFGPVREVEGYTPARWPQRPATAQLVAAYF
DQVRGTAASNLVTWSVKDGPDGLVTGPDEQADPVGQPGYGRC
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory