SitesBLAST
Comparing WP_011606633.1 NCBI__GCF_000058485.1:WP_011606633.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
27% identity, 62% coverage: 185:483/484 of query aligns to 97:400/404 of 3wx9A
- binding 2-oxoglutaric acid: D213 (= D296), P214 (≠ W297), Y215 (≠ V298), G216 (≠ A299), E217 (≠ P300), G241 (≠ L324), T242 (≠ S325), I246 (≠ S329)
- binding (2E)-pent-2-enedioic acid: Y130 (= Y218), N184 (= N267), R376 (= R459)
- binding glutamic acid: L131 (≠ P219), V360 (≠ G443), A364 (= A447), R369 (≠ E452)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G192), S105 (≠ G193), Q106 (= Q194), Y130 (= Y218), N184 (= N267), D212 (= D295), P214 (≠ W297), Y215 (≠ V298), T242 (≠ S325), S244 (= S327), K245 (= K328), R252 (= R335)
Sites not aligning to the query:
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
27% identity, 62% coverage: 185:483/484 of query aligns to 97:400/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: L131 (≠ P219), Q135 (≠ A223), A364 (= A447), R369 (≠ E452)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: Y130 (= Y218), L131 (≠ P219), A132 (≠ G220), N184 (= N267), R376 (= R459)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G192), S105 (≠ G193), Q106 (= Q194), Y130 (= Y218), V179 (≠ Q262), N184 (= N267), D212 (= D295), P214 (≠ W297), Y215 (≠ V298), T242 (≠ S325), S244 (= S327), K245 (= K328), R252 (= R335)
Sites not aligning to the query:
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
27% identity, 62% coverage: 185:483/484 of query aligns to 97:400/404 of 3aowC
- binding 2-oxoglutaric acid: Y130 (= Y218), L275 (= L358)
- binding pyridoxal-5'-phosphate: G104 (= G192), S105 (≠ G193), Q106 (= Q194), Y130 (= Y218), V179 (≠ Q262), N184 (= N267), D212 (= D295), P214 (≠ W297), Y215 (≠ V298), T242 (≠ S325), S244 (= S327), K245 (= K328), R252 (= R335)
Sites not aligning to the query:
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
27% identity, 62% coverage: 185:483/484 of query aligns to 97:400/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G192), S105 (≠ G193), Q106 (= Q194), Y130 (= Y218), V179 (≠ Q262), N184 (= N267), D212 (= D295), P214 (≠ W297), Y215 (≠ V298), T242 (≠ S325), S244 (= S327), K245 (= K328), R252 (= R335)
5tf5A Crystal structure of human kat-2 in complex with a reversible inhibitor
27% identity, 79% coverage: 103:484/484 of query aligns to 8:424/425 of 5tf5A
2r2nA The crystal structure of human kynurenine aminotransferase ii in complex with kynurenine (see paper)
27% identity, 79% coverage: 103:484/484 of query aligns to 8:424/425 of 2r2nA
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: I19 (vs. gap), R20 (≠ H114), Y142 (= Y218), R399 (= R459)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S117 (≠ G193), Q118 (= Q194), Y142 (= Y218), N202 (= N267), D230 (= D295), P232 (≠ W297), Y233 (≠ V298), S260 (= S325), S262 (= S327), K263 (= K328), R270 (= R335)
6t8qA Hkatii in complex with ligand (2r)-n-benzyl-1-[6-methyl-5-(oxan-4-yl)- 7-oxo-6h,7h-[1,3]thiazolo[5,4-d]pyrimidin-2-yl]pyrrolidine-2- carboxamide (see paper)
27% identity, 79% coverage: 103:484/484 of query aligns to 11:427/428 of 6t8qA
4gebA Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 79% coverage: 103:484/484 of query aligns to 8:424/428 of 4gebA
- binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: G39 (≠ A133), L40 (≠ Y134), Y74 (≠ R161), S117 (≠ G193), Q118 (= Q194), Y142 (= Y218), N202 (= N267), D230 (= D295), P232 (≠ W297), Y233 (≠ V298), S260 (= S325), S262 (= S327), K263 (= K328), R270 (= R335), R399 (= R459)
4ge9A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 79% coverage: 103:484/484 of query aligns to 8:424/428 of 4ge9A
- binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: I19 (vs. gap), R20 (≠ H114), T23 (= T117), G39 (≠ A133), L40 (≠ Y134), Y74 (≠ R161), S75 (≠ T162), S77 (≠ V164), S117 (≠ G193), Q118 (= Q194), Y142 (= Y218), N202 (= N267), D230 (= D295), P232 (≠ W297), Y233 (≠ V298), S260 (= S325), S262 (= S327), R270 (= R335), L293 (= L358), R399 (= R459)
4ge7A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 79% coverage: 103:484/484 of query aligns to 8:424/428 of 4ge7A
- binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-6-phenoxy-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: I19 (vs. gap), G39 (≠ A133), Y74 (≠ R161), S75 (≠ T162), S77 (≠ V164), S117 (≠ G193), Q118 (= Q194), Y142 (= Y218), N202 (= N267), D230 (= D295), P232 (≠ W297), Y233 (≠ V298), S260 (= S325), S262 (= S327), R270 (= R335), L293 (= L358), R399 (= R459)
4gdyB Kynurenine aminotransferase ii inhibitors
27% identity, 79% coverage: 103:484/484 of query aligns to 8:424/428 of 4gdyB
- binding (5-hydroxy-6-methyl-4-{[(1-oxo-7-phenoxy-1,2-dihydro[1,2,4]triazolo[4,3-a]quinolin-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: I19 (vs. gap), M33 (≠ V127), G39 (≠ A133), Y74 (≠ R161), S75 (≠ T162), G116 (= G192), S117 (≠ G193), Q118 (= Q194), Y142 (= Y218), N202 (= N267), D230 (= D295), P232 (≠ W297), S260 (= S325), S262 (= S327), R270 (= R335), R399 (= R459)
8tn3A Structure of s. Hygroscopicus aminotransferase mppq complexed with pyridoxamine 5'-phosphate (pmp) (see paper)
35% identity, 73% coverage: 124:477/484 of query aligns to 18:383/388 of 8tn3A
2vgzA Crystal structure of human kynurenine aminotransferase ii (see paper)
27% identity, 68% coverage: 158:484/484 of query aligns to 60:413/414 of 2vgzA
2xh1A Crystal structure of human kat ii-inhibitor complex (see paper)
27% identity, 68% coverage: 158:484/484 of query aligns to 58:411/412 of 2xh1A
- binding (3s)-10-(4-aminopiperazin-1-yl)-9-fluoro-7-hydroxy-3-methyl-2,3-dihydro-8h-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylate: Y61 (≠ R161), L280 (= L358)
- binding pyridoxal-5'-phosphate: S104 (≠ G193), Q105 (= Q194), Y129 (= Y218), D217 (= D295), P219 (≠ W297), S247 (= S325), S249 (= S327), R257 (= R335)
Sites not aligning to the query:
3ue8A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 68% coverage: 158:484/484 of query aligns to 54:407/410 of 3ue8A
- binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: Y57 (≠ R161), S100 (≠ G193), Q101 (= Q194), Y125 (= Y218), N185 (= N267), D213 (= D295), P215 (≠ W297), Y216 (≠ V298), S243 (= S325), S245 (= S327), K246 (= K328), R253 (= R335), R382 (= R459)
Sites not aligning to the query:
4ge4A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 79% coverage: 103:484/484 of query aligns to 8:418/422 of 4ge4A
- binding (5-hydroxy-4-{[(1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: I19 (vs. gap), G34 (≠ S132), G35 (≠ A133), L36 (≠ Y134), Y68 (≠ R161), G110 (= G192), S111 (≠ G193), Q112 (= Q194), Y136 (= Y218), N196 (= N267), D224 (= D295), P226 (≠ W297), Y227 (≠ V298), S254 (= S325), S256 (= S327), K257 (= K328), R264 (= R335), R393 (= R459)
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
32% identity, 74% coverage: 126:482/484 of query aligns to 29:387/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: G35 (≠ S132), G36 (≠ A133), G95 (= G192), S96 (≠ G193), Q97 (= Q194), Y121 (= Y218), N170 (= N267), D198 (= D295), Y201 (≠ V298), S231 (= S325), S233 (= S327), K234 (= K328), R241 (= R335), R364 (= R459)
Sites not aligning to the query:
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
32% identity, 74% coverage: 126:482/484 of query aligns to 29:387/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G192), S96 (≠ G193), Q97 (= Q194), Y121 (= Y218), N170 (= N267), D198 (= D295), A200 (≠ W297), Y201 (≠ V298), S231 (= S325), S233 (= S327), K234 (= K328), R241 (= R335)
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
32% identity, 74% coverage: 125:482/484 of query aligns to 24:383/389 of 2z1yA
- binding leucine: G32 (≠ A133), Y117 (= Y218), R360 (= R459)
- binding pyridoxal-5'-phosphate: G91 (= G192), S92 (≠ G193), Q93 (= Q194), Y117 (= Y218), N166 (= N267), D194 (= D295), Y197 (≠ V298), S227 (= S325), S229 (= S327), K230 (= K328), R237 (= R335)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
32% identity, 74% coverage: 126:482/484 of query aligns to 33:391/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: G39 (≠ S132), G40 (≠ A133), G99 (= G192), S100 (≠ G193), Q101 (= Q194), Y125 (= Y218), N174 (= N267), D202 (= D295), Y205 (≠ V298), S235 (= S325), S237 (= S327), K238 (= K328), R245 (= R335), R368 (= R459)
Sites not aligning to the query:
Query Sequence
>WP_011606633.1 NCBI__GCF_000058485.1:WP_011606633.1
MYEDIGGSADGPAAGPGRGGGPGGGGGPAGIVDELTALCARLPAGSALPSTRELQRRHRA
SALTVQRALAAVAARGLLLTTPGRGSVVAAPPASRSTDLSWQTAVLGARDDLPHDMTDLY
SPVPDGVLDLASAYLDPAAQPVGLLAAAAARAARRPGAWDRTPVAGLPDLRGLIAGLVSP
SHTASDVLVTSGGQAALALVFRALGRPGEALAVEHTTYPGALAAARAAGLVPVPVPADGA
GVRPDDLADVLGRSGARLVYLQPRHANPTGAVLAPDRRPAVLAALRDHRAFCVEDDWVAP
LDLTAVSPPPLAAADPDGHVVHVLSLSKSVSPGLRIAAVTARGPVAARLARARITDDLFV
APVLQQTAVELLSTPGWRRHLASLRRVLLARRAALLAAIARALPTLGPPQPPSGGVHVWL
PLPPGADDRLVTAAARRAGVAVGPGSAYTVGEVVAPHLRLTYSRTPGEGLVAAVDRLAAV
LHDG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory