SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (≠ R30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (= H83) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H108) mutation to H: Little effect on the kinetic properties.
E349 (= E383) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
28% identity, 88% coverage: 3:539/613 of query aligns to 2:433/497 of 1ct9A

query
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1ct9A

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active site: L50 (= L51), N74 (≠ S77), G75 (= G78), T305 (vs. gap), R308 (vs. gap), E332 (= E383), M366 (≠ G450)
binding adenosine monophosphate: L232 (= L262), L233 (= L263), S234 (= S264), S239 (= S269), A255 (= A294), V256 (= V295), D263 (= D314), M316 (≠ L368), S330 (= S381), G331 (= G382), E332 (= E383)
binding glutamine: R49 (= R50), L50 (= L51), I52 (≠ V53), V53 (≠ I54), N74 (≠ S77), G75 (= G78), E76 (= E79), D98 (= D102)

Sites not aligning to the query:

active site: 1
binding glutamine: 1

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 88% coverage: 1:539/613 of query aligns to 1:468/557 of P78753

query
sites
P78753

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S391 (≠ T467) modified: Phosphoserine

Sites not aligning to the query:

489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
26% identity, 65% coverage: 1:397/613 of query aligns to 1:379/561 of P08243

query
sites
P08243

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G

G

V

V

K

R

P

P

L

L

F

F

Y

K

A

A

E

M

I

T

P

E

G

D

G

G

V

F

V

L

-

A

F

V

G

C

S

S

E

E

P

A

K

K

A

G

L

L

F

V

C

T

-

L

H

K

P

H

L

S

V

A

R

T

P

P

R

F

V

L

N

K

A

V

D

E

G

P

L

F

R

L

E

P

A

G

Y

H

S

Y

L

E

L

V

F

L

N

D

T

L

G

K

P

P

T

N

V

G

W

K

T

V

G

A

V

S

R

V

E

E

L

M

A

V

P

K

G

Y

A

H

L

H

A

C

T

R

F

D

G
x
V

P

P

-

L

H

H

G

A

L

L

T

Y

E

D

R

N

T

V

Y

E

W

K

A

L

L

F

D

P

A

G

A

F

A

E

M

I

A

E

A

T

V

V

S

K

P

-

Q

-

A

-

V

-

E

N

Q

N

V

L

R

R

V

I

H

L

L

F

E

N

R

N

A

A

T

V

A

K

Q

R

L

L

E

M

A

T

D

D

V

R

P

R

L

I

C

G

S

C

L

L

S

S

G

G

L

L

D

D

S

S

T

S

V

L

L

V

T

A

A

A

L

T

L

L

A

L

D

K

E

Q

L

L

R

K

R

E

R

A

E

Q

G

V

P

Q

H

Y

A

P

R

-

I

L

R

Q

S

T

F

F

A

A

V

I

D

G

Y

M

S

E

D

D

Q

-

A

-

V

-

Q

-

F

-

T

S

G

P

D

D

V

L

L

L

R

A

T

-

G

-

H

-

D

-

A

-

P

-

Y

-

A

A

V

R

E

K

A

V

S

A

R

D

Y

H

I

I

G

G

T

S

D

E

H

H

S

Y

T

E

V

V

V

L

L

F

D

N

P

S

H

E

T

E

L

G

L

I

D

Q

P

A

E

L

H

D

R

E

L

V

A

-

V

I

V

F

A

S

A

L

R

E

D

T

S

Y

P

D

I

I

G

T

V

T

G

V

D

R

M

A

D

S

T

V

S

G

L

M

Y

Y

V

L

L

I

F

S

G

K

K

Y

I

I

A

R

Q

K

S

N

-

T

-

D

S

S

T

V

V

V

A

I

L
x
F

S

S

G

G

E

E

A

G

A

S

D

D

E

E

V

L

F

T

G

Q

G

G

Y

Y

P

I

W

Y

F

F

H

H

S

K

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ V8) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ G212) to E: in dbSNP:rs1049674
F362 (≠ L380) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
25% identity, 65% coverage: 2:397/613 of query aligns to 1:366/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

T

-

G

-

W
|
W

V

A

S

L

F

F

G

G

Q

S

D

D

Y

C

E

L

Q

S

K

V

T

Q

V

C

I

L

E

S

A

A

M

M

T

K

A

-

T

-

L

I

R

A

R

H

R

R

G

G

P

P

D

D

A

A

-

F

-

R

-

F

G

E

G

N

T

V

W

N

V

G

G

Y

A

T

H

N

A

C

I

F

G

G

H

F

R

H

R
|
R

L
|
L

A

A

V
|
V

I
x
V

D

D

L

P

V

L

G

F

G

G

V

M

Q

Q

P

P

M

-

V

I

S

R

D

V

Q

K

D

K

G

Y

A

P

E

Y

T

L

V

W

L

L

T

C

Y
|
Y

S
x
N

G
|
G

E
|
E

I

I

Y

Y

N

N

H

H

H

K

E

K

L

M

R

Q

G

Q

E

H

L

F

V

-

R

-

G

E

H

F

T

E

L

Y

R

Q

T

T

R

K

S
x
V

D
|
D

T

G

E

E

V

I

L

L

H

H

A

L

Y

Y

L

D

E

K

W

G

G

G

-

I

D

E

L

Q

L

T

V

I

D

C

R

M

L

L

E

D

G

G

M

V

Y

F

A

A

F

F

A

V

V

L

W

L

D

D

E

T

R

A

A

N

G

K

R

K

L

V

L

F

L

L

V

G

R

R

D

D

R

T

L

Y

G

G

V

V

K

R

P

P

L

L

F

F

Y

K

A

A

E

M

I

T

P

E

G

D

G

G

V

F

V

L

-

A

F

V

G

C

S

S

E

E

P

A

K

K

A

G

L

L

F

-

C

-

H

-

P

-

L

-

V

-

R

-

P

-

R

-

V

-

N

-

A

-

D

-

G

-

L

-

R

-

E

-

A

-

Y

-

S

-

L

V

L

T

F

L

N

K

T

H

G

S

P

A

T

T

V

P

W

F

T

L

G

K

V

V

R

E

E

P

L

F

A

L

P

P

G

G

-

H

-

Y

A

E

L

V

A

L

T

D

F

L

G

K

P

P

H

N

G

G

L

K

-

V

-

A

-

S

T

V

E

E

R

M

T

V

Y

K

W

Y

A

H

L

H

D

C

A

R

A

D

A

V

M

F

A

P

A

G

V

F

S

E

P

I

Q

E

A

T

A

V

V

K

E

N

Q

N

V

L

R

R

V

I

H

L

L

F

E

N

R

N

A

A

T

V

A

K

Q

R

L

L

E

M

A

T

D

D

V

R

P

R

L

I

C

G

S

C

L

L

S

S

G

G

L

L

D

D

S

S

T

S

V

L

L

V

T

A

A

A

L

T

L

L

A

L

D

K

E

Q

L

L

R

K

R

E

R

A

E

Q

G

V

P

Q

H

Y

A

P

R

-

I

L

R

Q

S

T

F

F

A

A

V

I

D

G

Y

M

S

E

D

D

Q

S

A

P

V

-

Q

-

F

-

T

-

G

-

D

-

V

-

L

-

R

-

T

-

G

-

H

-

D

D

A

L

P

L

Y

A

A

A

V

R

E

K

A

V

S

A

R

D

Y

H

I

I

G

G

T

S

D

E

H

H

S

Y

T

E

V

V

V

L

L

F

D

N

P

S

H

E

T

E

L

G

L

I

D

Q

P

A

E

L

H

D

R

E

L

V

A

-

V

I

V

F

A

S

A

L

R

E

D

T

S

Y

P

D

I

I

G
x
T

V

T

G

V

D
x
R

M

A

D

S

T

V

S

G

L

M

Y

Y

V

L

L

I

F

S

G

K

K

Y

I

I

A

R

Q

K

S

N

-

T

-

D

S

S

T

V

V

V

A

I

L

F

S

S

G

G

E

E

A

G

A

S

D

D

E

E

V

L

F

T

G

Q

G

G

Y

Y

P

I

W

Y

F

F

H

H

S

K

active site: W4 (= W7), L49 (= L51), N74 (≠ S77), G75 (= G78), T324 (≠ G357), R327 (≠ D360)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R50), V51 (= V53), V52 (≠ I54), Y73 (= Y76), N74 (≠ S77), G75 (= G78), E76 (= E79), V95 (≠ S101), D96 (= D102)

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
33% identity, 20% coverage: 45:167/613 of query aligns to 68:199/460 of 6lbpA

query
sites
6lbpA

A

A

I

I

G

G

H

H

R

V

R

R

L

Y

A

S

V

T

I

A

-

G

-

S

D

S

L

M

V

L

G

K

G

N

V

V

Q

Q

P

P

M

F

V

V

S

A

D

G

Q

Y

D

R

G

F

A

G

E

S

T

V

V

G

L

V

T

A

Y

H

S
x
N

G
|
G

E

N

I

L

Y

V

N

N

H

Y

H

T

E

K

L

L

R

R

G

A

E

D

L

L

V

E

R

E

R

N

G

G

H

S

T

I

L

F

R

N

T

T

R

S

S

S

D

D

T

T

E

E

V

V

L

L

H

H

A

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

-

F

Y

F

L

M

E

R

W

I

G

V

D

D

L

A

L

C

V

-

D

E

R

K

L

L

E

Q

G

G

M

A

Y

Y

A

S

F

M

A

V

V

F

W

V

D

T

E

E

R

-

A

-

G

D

R

K

L

L

L

V

L

A

V

V

R

R

D

D

R

P

L

H

G

G

V

F

K

R

P

P

L

L

F

V

Y

M

A

G

-

R

E

R

I

S

P

N

G

G

G

A

V

V

F

F

G

A

S

S

E

E

P

T

K

C

A

A

L

L

active site: N102 (≠ S77), G103 (= G78)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
33% identity, 20% coverage: 45:167/613 of query aligns to 154:285/561 of Q9STG9

query
sites
Q9STG9

A

A

I

I

G

G

H

H

R

V

R

R

L

Y

A

S

V

T

I

A

-

G

-

S

D

S

L

M

V

L

G

K

G

N

V

V

Q

Q

P

P

M

F

V

V

S

A

D

G

Q

Y

D

R

G

F

A

G

E

S

T

V

V

G

L

V

T

A

Y
x
H

S

N

G

G

E

N

I

L

Y

V

N

N

H

Y

H

T

E

K

L

L

R

R

G

A

E

D

L

L

V

E

R

E

R

N

G

G

H

S

T

I

L

F

R

N

T

T

R

S

S

S

D

D

T

T

E

E

V

V

L

L

H

H

A

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

-

F

Y

F

L

M

E

R

W

I

G

V

D

D

L

-

L

A

V

C

D

E

R

K

L

L

E

Q

G

G

M

A

Y

Y

A

S

F

M

A

V

V

F

W

V

D

T

E

E

R

-

A

-

G

D

R

K

L

L

L

V

L

A

V

V

R

R

D

D

R

P

L

H

G

G

V

F

K
x
R

P
|
P

L

L

F

V

Y

M

A

G

-

R

E

R

I

S

P

N

G

G

G

A

V

V

F

F

G

A

S

S

E

E

P

T

K

C

A

A

L

L

H187 (≠ Y76) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K147) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P148) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

1mb9A Beta-lactam synthetase complexed with atp (see paper)
31% identity, 47% coverage: 69:357/613 of query aligns to 62:343/485 of 1mb9A

query
sites
1mb9A

G

G

A

A

E

P

T

T

V

T

L

A

T

V

Y

L

S
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

H

D

E

E

L

L

R

L

G

S

E

V

L

L

V

-

R

-

G

P

H

A

T

G

L

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

V

V

L

L

H

R

A

-

Y

L

L

L

E

E

W

R

G

Y

D

D

L

L

L

H

V

A

D

F

R

R

L

L

-

V

E

N

G

G

M

R

Y

F

A

A

F

T

A

V

V

V

W

-

D

-

E

-

R

R

A

T

G

G

-

D

R

R

L

V

L

L

V

A

R

T

D

D

R

H

L

A

G

G

V

S

K

V

P

P

L

L

F

Y

Y

T

A

C

E

V

I

A

P

P

G

G

G

E

V

V

V

R

F

A

G

S

S

T

E

E

P

A

K

K

A

A

L

L

F

A

C

A

H

F

P

P

L

L

V

-

R

-

P

-

R

-

V

-

N

-

A

A

D

D

G

A

L

R

R

R

E

V

A

A

Y

G

S

-

L

-

F

-

N

-

T

-

G

-

P

-

T

-

V

-

W

L

T

T

G

G

V

V

R

Y

E

Q

L

V

A

P

P

A

G

G

A

A

L

V

A

M

T

D

F

I

G

D

P

L

H

G

G

S

L

G

T

T

E

A

R

V

T

T

Y

H

W

R

A

T

L

W

D

T

A

P

A

G

A

L

M

S

A

R

V

I

S

L

P

P

Q

E

A

G

-

E

A

A

V

V

E

A

Q

A

V

V

R

R

V

A

H

A

L

L

E

E

R

K

A

A

T

V

A

A

A

Q

Q

R

L

V

E

T

-

P

A

G

D

D

V

T

P

P

L

L

C

V

S

-

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

T

S

V

G

L

V

T

A

A

A

L

C

L

A

A

-

D

-

E

-

L

-

R

-

R

H

R

R

E

A

G

A

P

G

H

E

A

L

R

D

I

T

R

V

S
|
S

F
x
M

A

G

V

T

D

D

Y

T

S

S

D

N

Q

E

-

F

-

R

A

E

V

A

Q

R

F

A

T

V

G

V

D

D

V

H

L

L

R

R

T

T

G

R

H

H

-

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

D

Q

A

L

P

P

Y

Y

A

A

V

V

E

W

A

A

S

S

R

E

Y

S

I

V

G

D

T

P

D
|
D

H

I

S

I

T

E

V
x
Y

V

L

L

L

D

-

P

P

H
x
L

T

T

L

A

L

L

-

Y

-

R

-

A

-

L

-

D

D

G

P

P

E

E

H

R

R

R

L

I

A

L

V

T

V
x
G

A
x
Y

A

G

R

A

D
|
D

S

I

P

P

I

L

G

G

active site: A70 (≠ S77), G71 (= G78), D310 (= D328), Y336 (≠ A350)
binding adenosine monophosphate: V235 (≠ L262), L236 (= L263), S242 (= S269), S260 (= S292), M261 (≠ F293), Y314 (≠ V332), L318 (≠ H337), G335 (≠ V349), Y336 (≠ A350)
binding adenosine-5'-triphosphate: V235 (≠ L262), L236 (= L263), S237 (= S264), G239 (= G266), D241 (= D268), S242 (= S269), S260 (= S292), M261 (≠ F293), L318 (≠ H337), G335 (≠ V349), D339 (= D353)
binding magnesium ion: D241 (= D268), D339 (= D353)
binding pyrophosphate 2-: S237 (= S264), G239 (= G266), D241 (= D268), S242 (= S269), D339 (= D353)

Sites not aligning to the query:

active site: 370, 431
binding adenosine-5'-triphosphate: 411, 431
binding pyrophosphate 2-: 411, 431

1jgtB Crystal structure of beta-lactam synthetase (see paper)
31% identity, 47% coverage: 69:357/613 of query aligns to 65:352/500 of 1jgtB

query
sites
1jgtB

G

G

A

A

E

P

T

T

V

T

L

A

T

V

Y

L

S
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

H

D

E

E

L

L

R

L

G

S

E

V

L

L

V

P

R

-

G

-

H

A

T

G

L

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

V

V

L

L

H

R

A

-

Y

L

L

L

E

E

W

R

G

Y

D

D

L

L

L

H

V

A

D

F

R

R

L

L

-

V

E

N

G

G

M

R

Y

F

A

A

F

T

A

V

V

V

W

-

D

-

E

-

R

R

A

T

G

G

-

D

R

R

L

V

L

L

V

A

R

T

D

D

R

H

L

A

G

G

V

S

K

V

P

P

L

L

F

Y

Y

T

A

C

E

V

I

A

P

P

G

G

G

E

V

V

V

R

F

A

G

S

S

T

E

E

P

A

K

K

A

A

L

L

F

A

C

A

H

H

P

R

L

D

V

P

R

K

P

G

R

F

V

P

N

L

A

A

D

D

G

A

L

R

R

R

E

V

A

A

Y

G

S

-

L

-

F

-

N

-

T

-

G

-

P

-

T

-

V

-

W

L

T

T

G

G

V

V

R

Y

E

Q

L

V

A

P

P

A

G

G

A

A

L

V

A

M

T

D

F

I

G

D

P

L

H

G

G

S

L

G

T

T

E

A

R

V

T

T

Y

H

W

R

A

T

L

W

D

T

A

P

A

G

A

L

M

S

A

R

V

I

S

L

P

P

Q

E

A

G

-

E

A

A

V

V

E

A

Q

A

V

V

R

R

V

A

H

A

L

L

E

E

R

K

A

A

T

V

A

A

A

Q

Q

R

L

V

E

T

-

P

A

G

D

D

V

T

P

P

L

L

C

V

S

-

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

T

S

V

G

L

V

T

A

A

A

L

C

L

A

A

-

D

-

E

-

L

-

R

-

R

H

R

R

E

A

G

A

P

G

H

E

A

L

R

D

I

T

R

V

S
|
S

F
x
M

A

G

V

T

D

D

Y

T

S

S

D

N

Q

E

-

F

-

R

A

E

V

A

Q

R

F

A

T

V

G

V

D

D

V

H

L

L

R

R

T

T

G

R

H

H

-

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

D

Q

A

L

P

P

Y

Y

A

A

V

V

E

W

A

A

S

S

R

E

Y

S

I

V

G

D

T

P

D
|
D

H

I

S

I

T

E

V
x
Y

V

L

L

L

D

-

P

P

H
x
L

T

T

L

A

L

L

-

Y

-

R

-

A

-

L

-

D

D

G

P

P

E

E

H

R

R

R

L

I

A

L

V

T

V
x
G

A
x
Y

A
x
G

R

A

D
|
D

S
x
I

P

P

I

L

G

G

active site: A73 (≠ S77), G74 (= G78), D319 (= D328), Y345 (≠ A350)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ L262), L245 (= L263), S246 (= S264), G248 (= G266), I249 (≠ L267), D250 (= D268), S251 (= S269), S269 (= S292), M270 (≠ F293), L327 (≠ H337), G344 (≠ V349), Y345 (≠ A350), D348 (= D353)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ V332), Y345 (≠ A350), G346 (≠ A351), D348 (= D353), I349 (≠ S354)
binding magnesium ion: D250 (= D268), D348 (= D353)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 354, 370, 379

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
32% identity, 47% coverage: 69:357/613 of query aligns to 61:344/496 of 1mbzA

query
sites
1mbzA

G

G

A

A

E

P

T

T

V

T

L

A

T

V

Y

L

S
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

H

D

E

E

L

L

R

L

G

S

E

V

L

L

V

-

R

-

G

P

H

A

T

G

L

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

V

V

L

L

H

R

A

-

Y

L

L

L

E

E

W

R

G

Y

D

D

L

L

L

H

V

A

D

F

R

R

L

L

-

V

E

N

G

G

M

R

Y

F

A

A

F

T

A

V

V

V

W

-

D

-

E

-

R

R

A

T

G

G

-

D

R

R

L

V

L

L

V

A

R

T

D

D

R

H

L

A

G

G

V

S

K

V

P

P

L

L

F

Y

Y

T

A

C

E

V

I

A

P

P

G

G

G

E

V

V

V

R

F

A

G

S

S

T

E

E

P

A

K

K

A

A

L

L

F

A

C

A

H

H

-

G

-

F

P

P

L

L

V

-

R

-

P

-

R

-

V

-

N

-

A

A

D

D

G

A

L

R

R

R

E

V

A

A

Y

G

S

-

L

-

F

-

N

-

T

-

G

-

P

-

T

-

V

-

W

L

T

T

G

G

V

V

R

Y

E

Q

L

V

A

P

P

A

G

G

A

A

L

V

A

M

T

D

F

I

G

D

P

L

H

G

G

S

L

G

T

T

E

A

R

V

T

T

Y

H

W

R

A

T

L

W

D

T

A

P

A

G

A

L

M

S

A

R

V

I

S

L

P

P

Q

E

A

G

-

E

A

A

V

V

E

A

Q

A

V

V

R

R

V

A

H

A

L

L

E

E

R

K

A

A

T

V

A

A

A

Q

Q

R

L

V

E

T

-

P

A

G

D

D

V

T

P

P

L

L

C

V

S

-

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

T

S

V

G

L

V

T

A

A

A

L

C

L

A

A

-

D

-

E

-

L

-

R

-

R

H

R

R

E

A

G

A

P

G

H

E

A

L

R

D

I

T

R

V

S
|
S

F
x
M

A

G

V

T

D

D

Y

T

S

S

D

N

Q

E

-

F

-

R

A

E

V

A

Q

R

F

A

T

V

G

V

D

D

V

H

L

L

R

R

T

T

G

R

H

H

-

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

D

Q

A

L

P

P

Y

Y

A

A

V

V

E

W

A

A

S

S

R

E

Y

S

I

V

G

D

T

P

D
|
D

H

I

S

I

T

E

V
x
Y

V

L

L

L

D

-

P

P

H
x
L

T

T

L

A

L

L

-

Y

-

R

-

A

-

L

-

D

D

G

P

P

E

E

H

R

R

R

L

I

A

L

V

T

V
x
G

A
x
Y

A
x
G

R

A

D
|
D

S
x
I

P

P

I

L

G

G

active site: A69 (≠ S77), G70 (= G78), D311 (= D328), Y337 (≠ A350)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ L262), L237 (= L263), S238 (= S264), S243 (= S269), S261 (= S292), M262 (≠ F293), Y315 (≠ V332), L319 (≠ H337), G336 (≠ V349), Y337 (≠ A350), G338 (≠ A351), D340 (= D353), I341 (≠ S354)
binding magnesium ion: D242 (= D268), D340 (= D353)
binding pyrophosphate 2-: S238 (= S264), G240 (= G266), D242 (= D268), S243 (= S269), D340 (= D353)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 362, 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
31% identity, 47% coverage: 69:357/613 of query aligns to 57:339/491 of 1mc1A

query
sites
1mc1A

G

G

A

A

E

P

T

T

V

T

L

A

T

V

Y

L

S
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

H

D

E

E

L

L

R

L

G

S

E

V

L

L

V

-

R

-

G

P

H

A

T

G

L

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

V

V

L

L

H

R

A

-

Y

L

L

L

E

E

W

R

G

Y

D

D

L

L

L

H

V

A

D

F

R

R

L

L

-

V

E

N

G

G

M

R

Y

F

A

A

F

T

A

V

V

V

W

-

D

-

E

-

R

R

A

T

G

G

-

D

R

R

L

V

L

L

V

A

R

T

D

D

R

H

L

A

G

G

V

S

K

V

P

P

L

L

F

Y

Y

T

A

C

E

V

I

A

P

P

G

G

G

E

V

V

V

R

F

A

G

S

S

T

E

E

P

A

K

K

A

A

L

L

F

A

C

A

-

G

H

F

P

P

L

L

V

-

R

-

P

-

R

-

V

-

N

-

A

A

D

D

G

A

L

R

R

R

E

V

A

A

Y

G

S

-

L

-

F

-

N

-

T

-

G

-

P

-

T

-

V

-

W

L

T

T

G

G

V

V

R

Y

E

Q

L

V

A

P

P

A

G

G

A

A

L

V

A

M

T

D

F

I

G

D

P

L

H

G

G

S

L

G

T

T

E

A

R

V

T

T

Y

H

W

R

A

T

L

W

D

T

A

P

A

G

A

L

M

S

A

R

V

I

S

L

P

P

Q

E

A

G

-

E

A

A

V

V

E

A

Q

A

V

V

R

R

V

A

H

A

L

L

E

E

R

K

A

A

T

V

A

A

A

Q

Q

R

L

V

E

T

-

P

A

G

D

D

V

T

P

P

L

L

C

V

S

-

L
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

T

S

V

G

L

V

T

A

A

A

L

C

L

A

A

-

D

-

E

-

L

-

R

-

R

H

R

R

E

A

G

A

P

G

H

E

A

L

R

D

I

T

R

V

S
|
S

F
x
M

A

G

V

T

D

D

Y

T

S

S

D

N

Q

E

-

F

-

R

A

E

V

A

Q

R

F

A

T

V

G

V

D

D

V

H

L

L

R

R

T

T

G

R

H

H

-

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

D

Q

A

L

P

P

Y

Y

A

A

V

V

E

W

A

A

S

S

R

E

Y

S

I

V

G

D

T

P

D
|
D

H

I

S

I

T

E

V
x
Y

V

L

L

L

D

-

P

P

H

L

T

T

L

A

L

L

-

Y

-

R

-

A

-

L

-

D

D

G

P

P

E

E

H

R

R

R

L

I

A

L

V

T

V
x
G

A
x
Y

A
x
G

R

A

D
|
D

S
x
I

P

P

I

L

G

G

active site: A65 (≠ S77), G66 (= G78), D306 (= D328), Y332 (≠ A350)
binding adenosine monophosphate: V231 (≠ L262), S233 (= S264), S238 (= S269), S256 (= S292), M257 (≠ F293), G331 (≠ V349)
binding magnesium ion: D237 (= D268), D335 (= D353)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ V332), Y332 (≠ A350), G333 (≠ A351), I336 (≠ S354)
binding pyrophosphate 2-: S233 (= S264), G235 (= G266), D237 (= D268), S238 (= S269), D335 (= D353)

Sites not aligning to the query:

active site: 366, 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 357, 366, 427
binding pyrophosphate 2-: 407, 427, 428

Query Sequence

>WP_011606722.1 NCBI__GCF_000058485.1:WP_011606722.1
MCGITGWVSFGQDDYEQKTVIEAMTATLRRRGPDAGGTWVGAHAAIGHRRLAVIDLVGGV
QPMVSDQDGAETVLTYSGEIYNHHELRGELVRRGHTLRTRSDTEVVLHAYLEWGDLLVDR
LEGMYAFAVWDERAGRLLLVRDRLGVKPLFYAEIPGGVVFGSEPKALFCHPLVRPRVNAD
GLREAYSLLFNTGPTVWTGVRELAPGALATFGPHGLTERTYWALDAAAMAAVSPQAAVEQ
VRVHLERATAAQLEADVPLCSLLSGGLDSTVLTALLADELRRREGPHARIRSFAVDYSDQ
AVQFTGDVLRTGHDAPYAVEASRYIGTDHSTVVLDPHTLLDPEHRLAVVAARDSPIGVGD
MDTSLYVLFGKIAQSSTVALSGEAADEVFGGYPWFHSPAALSAPTFPWLLVTGDEAAMPL
HPDLAATLRIEEFRADTYRDALAAVPHLDGEDPTEHRQREMAHLSLTRWLRQLLHRKDRL
SMAQGIEVRVPYCDHRLVEYAFGVPWAVRSFDGREKSLLRAAGAGRCPESVLIREKNHYP
TTHHPDYNRGLQAMALDALDAHGGRVRDLADVSRLRPLIDGPPERLEWGQRLSLERVVDL
ALWLDHHHPELTL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory