SitesBLAST
Comparing WP_011607008.1 NCBI__GCF_000058485.1:WP_011607008.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
68% identity, 81% coverage: 54:382/405 of query aligns to 9:339/360 of A0R220
- K151 (≠ E196) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
64% identity, 87% coverage: 50:402/405 of query aligns to 5:359/360 of P9WG59
- K69 (= K114) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N140) binding pyridoxal 5'-phosphate
- K151 (≠ E196) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (= GNAGN 240:244) binding pyridoxal 5'-phosphate
- T326 (= T369) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
67% identity, 81% coverage: 55:381/405 of query aligns to 1:329/349 of 2d1fA
- active site: K60 (= K114), T84 (= T138), D209 (≠ A261), R213 (= R265), L215 (≠ C267), A240 (= A292), T317 (= T369)
- binding pyridoxal-5'-phosphate: F59 (= F113), K60 (= K114), N86 (= N140), V186 (= V239), G187 (= G240), N188 (= N241), A189 (= A242), G190 (= G243), N191 (= N244), A240 (= A292), T317 (= T369)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
58% identity, 80% coverage: 57:380/405 of query aligns to 3:326/350 of 6nmxA
- active site: K60 (= K114), T84 (= T138), E216 (≠ Q270), S220 (≠ A274), A238 (= A292), T315 (= T369)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K114), S83 (= S137), T84 (= T138), N86 (= N140), T87 (= T141), F133 (= F187), N153 (= N207), S154 (= S208), R159 (= R213), V185 (= V239), G186 (= G240), N187 (= N241), A188 (= A242), G189 (= G243), N190 (= N244), A238 (= A292), I239 (= I293), E285 (= E339), T315 (= T369)
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
58% identity, 85% coverage: 57:402/405 of query aligns to 2:350/350 of 2zsjA
- active site: K61 (= K114), T85 (= T138), Q218 (= Q270), A222 (= A274), A240 (= A292), T317 (= T369)
- binding pyridoxal-5'-phosphate: F60 (= F113), K61 (= K114), N87 (= N140), V186 (= V239), G187 (= G240), N188 (= N241), A189 (= A242), G190 (= G243), N191 (= N244), A240 (= A292), T317 (= T369), G318 (= G370)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
58% identity, 80% coverage: 57:380/405 of query aligns to 1:324/345 of 6cgqB
- active site: K58 (= K114), T82 (= T138), E214 (≠ Q270), S218 (≠ A274), A236 (= A292), T313 (= T369)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K114), S81 (= S137), T82 (= T138), N84 (= N140), T85 (= T141), V183 (= V239), G184 (= G240), N185 (= N241), A186 (= A242), N188 (= N244), A236 (= A292), I237 (= I293), E283 (= E339), T313 (= T369)
- binding phosphate ion: K58 (= K114), T85 (= T141), N151 (= N207), S152 (= S208), R157 (= R213), N185 (= N241)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
57% identity, 80% coverage: 59:380/405 of query aligns to 1:316/339 of 6cgqA
- active site: K56 (= K114), T80 (= T138), E206 (≠ Q270), S210 (≠ A274), A228 (= A292), T305 (= T369)
- binding pyridoxal-5'-phosphate: F55 (= F113), K56 (= K114), N82 (= N140), V175 (= V239), G176 (= G240), N177 (= N241), A178 (= A242), G179 (= G243), N180 (= N244), A228 (= A292), E275 (= E339), T305 (= T369), G306 (= G370)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
61% identity, 78% coverage: 60:376/405 of query aligns to 5:324/350 of 1uimA
- active site: K61 (= K114), T85 (= T138), P212 (= P264), G216 (= G268), Q218 (= Q270), A240 (= A292), T317 (= T369)
- binding pyridoxal-5'-phosphate: F60 (= F113), K61 (= K114), N87 (= N140), G187 (= G240), N188 (= N241), A189 (= A242), G190 (= G243), N191 (= N244), A240 (= A292), E287 (= E339), T317 (= T369), G318 (= G370)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
61% identity, 78% coverage: 60:376/405 of query aligns to 4:323/350 of 3aeyA
- active site: K60 (= K114), T84 (= T138), P211 (= P264), G215 (= G268), Q217 (= Q270), A239 (= A292), T316 (= T369)
- binding sulfate ion: K60 (= K114), K60 (= K114), G85 (= G139), N86 (= N140), T87 (= T141), T87 (= T141), S154 (= S208), R159 (= R213), N187 (= N241), R228 (≠ E281), V230 (= V283), E231 (≠ T284), R232 (≠ S285), A239 (= A292)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
61% identity, 78% coverage: 60:376/405 of query aligns to 5:324/351 of 3aexA
- active site: K61 (= K114), T85 (= T138), P212 (= P264), G216 (= G268), Q218 (= Q270), A240 (= A292), T317 (= T369)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K114), S84 (= S137), T85 (= T138), N87 (= N140), T88 (= T141), V186 (= V239), G187 (= G240), N188 (= N241), A189 (= A242), G190 (= G243), N191 (= N244), A240 (= A292), I241 (= I293), E287 (= E339), T317 (= T369)
- binding phosphate ion: K61 (= K114), T88 (= T141), N154 (= N207), S155 (= S208), R160 (= R213), N188 (= N241)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
61% identity, 78% coverage: 60:376/405 of query aligns to 5:324/351 of 1v7cA
- active site: K61 (= K114), T85 (= T138), P212 (= P264), G216 (= G268), Q218 (= Q270), A240 (= A292), T317 (= T369)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K114), S84 (= S137), T85 (= T138), N87 (= N140), T88 (= T141), F134 (= F187), N154 (= N207), S155 (= S208), R160 (= R213), V186 (= V239), G187 (= G240), N188 (= N241), A189 (= A242), G190 (= G243), N191 (= N244), A240 (= A292), I241 (= I293), E287 (= E339), T317 (= T369)
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 76% coverage: 68:374/405 of query aligns to 155:477/526 of Q9S7B5
- N172 (≠ P85) binding S-adenosyl-L-methionine
- L173 (= L86) binding S-adenosyl-L-methionine
- K181 (≠ E94) binding in monomer B; binding in monomer A
- N187 (≠ C98) binding in monomer B
- L205 (≠ R116) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
36% identity, 76% coverage: 68:374/405 of query aligns to 80:402/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F113), K128 (= K114), D159 (≠ N140), G259 (≠ V239), G260 (= G240), N261 (= N241), L262 (≠ A242), G263 (= G243), N264 (= N244), A321 (= A292), H369 (≠ S341), T397 (= T369)
- binding s-adenosylmethionine: S90 (≠ T78), F92 (≠ R80), N97 (≠ P85), L98 (= L86), W100 (≠ L88), W115 (≠ H101), W115 (≠ H101), Q246 (≠ A227), F247 (≠ L228)
Sites not aligning to the query:
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
34% identity, 76% coverage: 68:374/405 of query aligns to 98:404/448 of 2c2gA
8y1jA Structure of the pyridoxal 5'-phosphate-dependent (plp) threonine deaminase ilva1 from pseudomonas aeruginosa pao1 (see paper)
30% identity, 71% coverage: 84:370/405 of query aligns to 20:305/502 of 8y1jA
Sites not aligning to the query:
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
28% identity, 73% coverage: 84:379/405 of query aligns to 23:314/319 of A4F2N8
- K53 (= K114) mutation to A: Loss of enzymatic activity.
Q8VBT2 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Mus musculus (Mouse)
27% identity, 72% coverage: 84:376/405 of query aligns to 11:294/327 of Q8VBT2
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
27% identity, 72% coverage: 84:375/405 of query aligns to 24:311/322 of 3l6bA
- active site: K54 (= K114), S77 (≠ T138), E203 (≠ R263), A207 (≠ C267), D209 (vs. gap), G232 (≠ A292), T278 (≠ S341), L305 (≠ T369), S306 (≠ G370)
- binding malonate ion: K54 (= K114), S76 (= S137), S77 (≠ T138), N79 (= N140), H80 (≠ S142), R128 (≠ C190), G232 (≠ A292)
- binding manganese (ii) ion: E203 (≠ R263), A207 (≠ C267), D209 (vs. gap)
- binding pyridoxal-5'-phosphate: F53 (= F113), K54 (= K114), N79 (= N140), G178 (= G240), G179 (≠ N241), G180 (≠ A242), G181 (= G243), M182 (≠ N244), V233 (≠ I293), E276 (= E339), T278 (≠ S341), S306 (≠ G370), G307 (≠ N371)
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
28% identity, 66% coverage: 84:350/405 of query aligns to 23:286/310 of 7nbgDDD
- active site: K53 (= K114), S76 (≠ T138), E202 (≠ R263), A206 (≠ C267), D208 (vs. gap), G231 (≠ A292)
- binding calcium ion: E202 (≠ R263), A206 (≠ C267), D208 (vs. gap)
- binding magnesium ion: N239 (≠ A299)
- binding ortho-xylene: S76 (≠ T138), Q81 (≠ S144), I96 (≠ V158), Y113 (≠ H176)
- binding pyridoxal-5'-phosphate: F52 (= F113), K53 (= K114), N78 (= N140), G177 (= G240), G178 (≠ N241), G179 (≠ A242), G180 (= G243), M181 (≠ N244), G231 (≠ A292), V232 (≠ I293), E275 (= E339), T277 (≠ S341)
Sites not aligning to the query:
6zspAAA serine racemase bound to atp and malonate. (see paper)
27% identity, 75% coverage: 84:387/405 of query aligns to 23:320/320 of 6zspAAA
- active site: K53 (= K114), S74 (≠ T138), E200 (≠ R263), A204 (≠ C267), D206 (vs. gap), G229 (≠ A292), L302 (≠ T369), S303 (≠ G370)
- binding adenosine-5'-triphosphate: S28 (≠ A89), S29 (≠ E90), I30 (≠ H91), K48 (≠ P109), T49 (= T110), Q79 (≠ S144), Y111 (≠ H176), E266 (≠ R332), R267 (= R333), K269 (≠ G335), N306 (≠ L373)
- binding magnesium ion: E200 (≠ R263), A204 (≠ C267), D206 (vs. gap)
- binding malonate ion: K53 (= K114), S73 (= S137), S74 (≠ T138), N76 (= N140), H77 (≠ S142), R125 (≠ C190), G229 (≠ A292), S232 (vs. gap)
Query Sequence
>WP_011607008.1 NCBI__GCF_000058485.1:WP_011607008.1
MTTTSTARAESSAVGASAAAGSSTAAESSARAQSGSFPGGPAAPAASTQPDRSHRPWRGI
IEEYRDRLPVDDDTPVITLREGGTPLLLAEHLSELTGCEVHLKVEGANPTGSFKDRGMTM
AISRAVGAGSQAVICASTGNTSASAAAYAARAGITCAVLVPSGKIALGKLAQALVHGARL
LQLDGSFDDCLRVARELADTSPVTLVNSVNPYRLEGQKTASFEIVEALGRAPDVHCLPVG
NAGNITAYWRGYLEEDARLDAGRPRMCGFQAAGAAPIVRGEIVTSPQTIATAIRIGNPAS
WDFAVDARDSSGGLIDAVNDRQILAAYRLLARREGVFVEPSSAASVAGLLMARADGRIEA
GQRVVCTVTGNGLKDPDWAISGAARPETIRPTVAGAAEALGLQTS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory