SitesBLAST
Comparing WP_011607176.1 NCBI__GCF_000058485.1:WP_011607176.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3jtxB Crystal structure of aminotransferase (np_283882.1) from neisseria meningitidis z2491 at 1.91 a resolution
34% identity, 56% coverage: 26:354/592 of query aligns to 32:391/393 of 3jtxB
2x5dD Crystal structure of a probable aminotransferase from pseudomonas aeruginosa (see paper)
32% identity, 55% coverage: 15:338/592 of query aligns to 9:358/380 of 2x5dD
- active site: Y113 (= Y116), D191 (= D176), A193 (≠ C178), K225 (= K212)
- binding pyridoxal-5'-phosphate: G87 (= G89), S88 (≠ T90), K89 (= K91), Y113 (= Y116), D191 (= D176), A193 (≠ C178), Y194 (= Y179), T222 (≠ S209), S224 (= S211), K225 (= K212), R233 (= R220)
2o1bA Structure of aminotransferase from staphylococcus aureus
28% identity, 56% coverage: 22:355/592 of query aligns to 18:372/376 of 2o1bA
- active site: Y115 (= Y116), D192 (= D176), A194 (≠ C178), K225 (= K212)
- binding pyridoxal-5'-phosphate: T90 (= T90), K91 (= K91), Y115 (= Y116), N164 (= N148), D192 (= D176), A194 (≠ C178), Y195 (= Y179), S222 (= S209), S224 (= S211), K225 (= K212), R233 (= R220)
6l1oB Product bound bacf structure from bacillus subtillis (see paper)
26% identity, 58% coverage: 10:354/592 of query aligns to 22:387/392 of 6l1oB
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G103 (≠ K91), K104 (≠ E92), Y128 (= Y116), N174 (= N144), D206 (= D176), Y209 (= Y179), S236 (= S209), S238 (= S211), K239 (= K212), R247 (= R220)
- binding tyrosine: Q39 (≠ V30), G40 (= G31), K104 (≠ E92), Y128 (= Y116), E130 (≠ T118), Y325 (= Y296), R367 (= R334)
Sites not aligning to the query:
6l1lB Apo-bacf structure from bacillus subtillis (see paper)
26% identity, 58% coverage: 10:354/592 of query aligns to 22:387/393 of 6l1lB
6l1nA Substrate bound bacf structure from bacillus subtillis (see paper)
26% identity, 58% coverage: 11:354/592 of query aligns to 19:386/393 of 6l1nA
- binding glycine: Y127 (= Y116), N177 (= N148), Y324 (= Y296), R366 (= R334)
- binding pyridoxal-5'-phosphate: G102 (≠ K91), Y127 (= Y116), N173 (= N144), N177 (= N148), D205 (= D176), Y208 (= Y179), S235 (= S209), S237 (= S211), R246 (= R220)
Sites not aligning to the query:
1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
27% identity, 55% coverage: 25:351/592 of query aligns to 27:378/388 of 1gdeA
- active site: K232 (= K212)
- binding glutamic acid: F120 (≠ Y116), N170 (= N148), R361 (= R334)
- binding pyridoxal-5'-phosphate: G94 (= G89), A95 (≠ T90), N96 (≠ K91), F120 (≠ Y116), N166 (= N144), D198 (= D176), Y201 (= Y179), S231 (= S211), K232 (= K212), R240 (= R220)
1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
27% identity, 55% coverage: 25:351/592 of query aligns to 27:378/388 of 1gd9A
- active site: K232 (= K212)
- binding pyridoxal-5'-phosphate: G94 (= G89), A95 (≠ T90), N96 (≠ K91), F120 (≠ Y116), N170 (= N148), D198 (= D176), V200 (≠ C178), Y201 (= Y179), S231 (= S211), K232 (= K212), R240 (= R220)
5yhvB Crystal structure of an aminotransferase from mycobacterium tuberculosis
30% identity, 59% coverage: 4:351/592 of query aligns to 12:380/387 of 5yhvB
- binding 2-oxoglutaric acid: Y80 (≠ L71), H84 (≠ L75), H205 (≠ I180), T215 (≠ P194), N229 (≠ H208)
- binding pyridoxal-5'-phosphate: Y63 (= Y54), G98 (= G89), S99 (≠ T90), S100 (≠ K91), Y124 (= Y116), N173 (= N148), D201 (= D176), V203 (≠ C178), Y204 (= Y179), S230 (= S209), S232 (= S211), K233 (= K212), R241 (= R220), I264 (≠ M245)
5yhvA Crystal structure of an aminotransferase from mycobacterium tuberculosis
30% identity, 59% coverage: 4:351/592 of query aligns to 19:387/394 of 5yhvA
- binding glutamic acid: H91 (≠ L75), H212 (≠ I180)
- binding pyridoxal-5'-phosphate: Y70 (= Y54), G105 (= G89), S106 (≠ T90), S107 (≠ K91), Y131 (= Y116), N180 (= N148), Y211 (= Y179), S237 (= S209), S239 (= S211), K240 (= K212), R248 (= R220), I271 (≠ M245)
P96847 Valine--pyruvate aminotransferase; Alanine--valine transaminase; EC 2.6.1.66 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
30% identity, 59% coverage: 4:351/592 of query aligns to 13:381/388 of P96847
- K234 (= K212) modified: N6-(pyridoxal phosphate)lysine
1j32A Aspartate aminotransferase from phormidium lapideum
27% identity, 53% coverage: 11:326/592 of query aligns to 17:357/388 of 1j32A
- active site: W124 (≠ Y116), D202 (= D176), I204 (≠ C178), K237 (= K212)
- binding pyridoxal-5'-phosphate: G98 (= G89), G99 (≠ T90), K100 (= K91), W124 (≠ Y116), N174 (= N148), D202 (= D176), I204 (≠ C178), Y205 (= Y179), K237 (= K212), R245 (= R220)
1v2fA Crystal structure of t.Th hb8 glutamine aminotransferase complex with 3-phenylpropionate (see paper)
29% identity, 55% coverage: 24:351/592 of query aligns to 26:364/368 of 1v2fA
- active site: F112 (≠ Y116), A181 (= A166), A183 (≠ E168), K222 (= K212)
- binding hydrocinnamic acid: Q32 (≠ V30), G33 (= G31), Y57 (= Y54), F112 (≠ Y116), F253 (= F243), R347 (= R334)
- binding pyridoxal-5'-phosphate: G86 (= G89), A87 (vs. gap), T88 (= T90), F112 (≠ Y116), N163 (= N148), D191 (= D176), V193 (≠ C178), Y194 (= Y179), S219 (= S209), K222 (= K212), R230 (= R220)
Sites not aligning to the query:
1v2eA Crystal structure of t.Th hb8 glutamine aminotransferase complex with a-keto-g-methylthiobutyrate (see paper)
29% identity, 55% coverage: 24:351/592 of query aligns to 26:364/368 of 1v2eA
- active site: F112 (≠ Y116), A181 (= A166), A183 (≠ E168), K222 (= K212)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: Q32 (≠ V30), G33 (= G31), F112 (≠ Y116), K222 (= K212), F309 (≠ Y296), R347 (= R334)
- binding pyridoxal-5'-phosphate: G86 (= G89), A87 (vs. gap), T88 (= T90), F112 (≠ Y116), D191 (= D176), V193 (≠ C178), Y194 (= Y179), S219 (= S209), K222 (= K212), R230 (= R220)
Sites not aligning to the query:
2o0rA The three-dimensional structure of n-succinyldiaminopimelate aminotransferase from mycobacterium tuberculosis (see paper)
28% identity, 59% coverage: 7:355/592 of query aligns to 8:384/385 of 2o0rA
P17139 Collagen alpha-1(IV) chain from Caenorhabditis elegans (see 3 papers)
39% identity, 20% coverage: 425:544/592 of query aligns to 1112:1233/1759 of P17139
Sites not aligning to the query:
- 141 P→L: In ju1197; suppresses the lethal phenotypes of the pxn-2 tm3464 mutant.
- 213 G→E: In b189; temperature-sensitive mutant. At the subrestrictive temperature of 22 degrees Celsius, causes the formation of ectopic presynaptic boutons on the ventral cord axons. Enhances the lethality of the pxn-2 ju436 mutant at 20 degrees Celsius.
- 258 G→E: In xd51; formation of ectopic presynaptic boutons on the ventral cord axons associated with a disruption of synapse basement membrane.
- 403 G→E: In allele g34; temperature-sensitive lethality during late embryogenesis.
- 409 G→E: In allele g23/hc70; temperature-sensitive lethality during late embryogenesis.
Q7SIB2 Collagen alpha-1(IV) chain from Bos taurus (Bovine) (see 3 papers)
40% identity, 14% coverage: 430:511/592 of query aligns to 490:584/1669 of Q7SIB2
Sites not aligning to the query:
- 204 modified: 3-hydroxyproline
- 207 modified: 3-hydroxyproline
- 210 modified: 3-hydroxyproline
- 587 modified: 3-hydroxyproline
- 602 modified: 3-hydroxyproline
- 605 modified: 3-hydroxyproline
- 647 modified: 3-hydroxyproline
- 1214 modified: 3-hydroxyproline
- 1424 modified: 3-hydroxyproline
- 1460 modified: Disulfide link with 1551
- 1493 modified: Disulfide link with 1548
- 1505 modified: Disulfide link with 1511
- 1511 modified: Disulfide link with 1505
- 1533 modified: S-Lysyl-methionine sulfilimine (Met-Lys) (interchain with K-1651)
- 1548 modified: Disulfide link with 1493
- 1551 modified: Disulfide link with 1460
- 1570 modified: Disulfide link with 1665
- 1604 modified: Disulfide link with 1662
- 1616 modified: Disulfide link with 1622
- 1622 modified: Disulfide link with 1616
- 1651 modified: S-Lysyl-methionine sulfilimine (Lys-Met) (interchain with M-1533)
- 1662 modified: Disulfide link with 1604
- 1665 modified: Disulfide link with 1570
P17140 Collagen alpha-2(IV) chain; Lethal protein 2 from Caenorhabditis elegans (see 4 papers)
42% identity, 17% coverage: 418:515/592 of query aligns to 812:909/1758 of P17140
- G877 (≠ N483) mutation to R: In g30; temperature-sensitive mutant. At the restrictive temperature, causes 90 percent lethality. At the permissive temperature of 16 degrees Celsius, causes the formation of ectopic presynaptic boutons on the ventral cord axons.
- G904 (= G510) mutation to R: In E1470; 94% lethal.
Sites not aligning to the query:
- 48 G→E: In MN114; 73% lethal.
- 366 A→T: In MN126; 100% lethal.
- 479 E→K: In ju1166; suppresses the lethal phenotypes of the pxn-2 tm3464 mutant.
- 570 G→E: In MN109; 37% lethal.
- 587 P→L: In ju1180; suppresses the lethal phenotypes of the pxn-2 tm3464 mutant.
- 588 G→R: In MN103 and MN151; 96% lethal.
- 597 G→R: In MN152; 50% lethal.
- 690 G→E: In MN129; 100% lethal.; G→R: In MN101; 100% lethal.
- 737 G→E: In MN143; 100% lethal.
- 1003 G→E: In MN139; 20% lethal.
- 1125 G→D: In g25; temperature-sensitive mutant. At the restrictive temperature of 25 degrees Celsius, causes 96 percent embryonic lethality. At the permissive temperature of 22.5 degrees Celsius, partially restores normal distal tip cell migration in a mig-17 (k174) mutant background.
- 1152 G→D: In MN147; 7% lethal.
- 1286 G→D: In g37 and b246; 9% lethal. Moderate reduction in basement membrane localization associated with a moderate accumulation in muscle cell cytoplasm. At the restrictive temperature of 25 degrees Celsius, causes a reduction of nid-1 recruitment to the gonad basement membrane. At the permissive temperature of 22.5 degrees Celsius, partially restores normal distal tip cell migration in a mig-17 (k174) mutant background.
- 1465 G→R: In k196; temperature-sensitive mutant. At the restrictive temperature of 25 degrees Celsius, causes 85 percent larval lethality. Slight reduction in basement membrane localization associated with a slight intracellular accumulation in muscle and distal tip cells (DTC). In a mig-17 (k174) mutant background, restores normal DTC migration and nid-1 basement membrane localization.
- 1610 S→L: In k193; temperature-sensitive mutant. At both the restrictive and permissive temperatures, causes 16-20 percent embryonic lethality. Slight reduction in basement membrane localization associated with a slight intracellular accumulation in muscle and distal tip cells (DTC). In a mig-17 (k174) mutant background, restores normal DTC migration and nid-1 basement membrane localization. Enhances the lethality of the pxn-2 mutant (ju432).
P02463 Collagen alpha-1(IV) chain from Mus musculus (Mouse) (see 2 papers)
39% identity, 14% coverage: 429:511/592 of query aligns to 489:584/1669 of P02463
Sites not aligning to the query:
- 602 modified: 3-hydroxyproline
- 603 modified: 4-hydroxyproline
- 605 modified: 3-hydroxyproline
- 606 modified: 4-hydroxyproline
- 623 modified: 4-hydroxyproline
- 626 modified: 4-hydroxyproline
- 629 modified: 4-hydroxyproline
- 632 modified: 4-hydroxyproline
- 1533 modified: S-Lysyl-methionine sulfilimine (Met-Lys) (interchain with K-1651)
- 1651 modified: S-Lysyl-methionine sulfilimine (Lys-Met) (interchain with M-1533)
P25940 Collagen alpha-3(V) chain from Homo sapiens (Human) (see 4 papers)
40% identity, 16% coverage: 426:518/592 of query aligns to 752:850/1745 of P25940
Sites not aligning to the query:
- 102 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 141 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 322 R → G: in dbSNP:rs2287803
- 349 modified: carbohydrate, O-linked (Xyl...) (chondroitin sulfate) serine
Query Sequence
>WP_011607176.1 NCBI__GCF_000058485.1:WP_011607176.1
MRLPDFPWDQLVSYKEKARTHPYGLVDLSVGTPVDSTPAVVQQALAGAADAPGYPLTVGT
PDLREAAAGWLARRLGVLVDPGAVLPVLGTKELVAQLPGQLGLEPGDRVWVPTPAYPTYE
VGALLARCEPVVGPADGVTLVWLNSPANPTGRVLTVDEMRAVVTWARERGVIVASDECYI
ELGWEARPVSVLHPDVCGGSHEGLLAVHSLSKRSNLAGYRAGFVTGDPQLVEGLTGVRKH
AGFMMPTPVAAAMAAAYADDIHVADQRARYANRRAVLAAALAVAGFTIDHSEAGLYLWAT
RGEDAWATVDALAGVGVLVAPGTFYGEGGRHHVRVALTAPDSQVATVPERMTMLAPAVVA
GGAGSHPTGPAGGGYPQPGPPGGYAAQQGGYAAAAPGYPAGPARVPYESAAGYETRPGYE
PRPGEGQPGYDGQPGYEGARGYEGQPRYEGQPTYQGQPDYLGQPDYQGQPTYQGQPDYQG
QPNYQGRAGYEGQPGYEGRRGFEGQRGYEGQPGYERAPRHARPSGYGARPGDHDDPARRD
GQAGYDHAGDYDSPAGYDRPPGPDDPPTGHGQPSGAGRHAAGGPWNSEPDIR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory