SitesBLAST
Comparing WP_011611983.1 NCBI__GCF_000014265.1:WP_011611983.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8Z0C8 L-lactate oxidase; LOX; Glyoxylate oxidase; No-LOX; EC 1.1.3.-; EC 1.2.3.5 from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
68% identity, 100% coverage: 1:359/359 of query aligns to 4:364/365 of Q8Z0C8
- M82 (= M79) mutation to T: Increases oxidation activity with both L-lactate and glycolate. Shows a 6-fold decrease in the L-lactate/glycolate oxidase activity ratio.
- L112 (≠ M109) mutation to W: Impairs oxidation of L-lactate. Shows a 2-fold decrease in the L-lactate/glycolate oxidase activity ratio.
- F212 (≠ V207) mutation to V: Impairs oxidation of L-lactate. Shows a 27-fold decrease in the L-lactate/glycolate oxidase activity ratio.
6gmcA 1.2 a resolution structure of human hydroxyacid oxidase 1 bound with fmn and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole
52% identity, 99% coverage: 5:359/359 of query aligns to 6:359/360 of 6gmcA
- active site: Y132 (= Y132), D160 (= D160), H258 (= H258)
- binding 5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole-4-carboxylate: Y26 (= Y25), M82 (≠ F81), W110 (≠ M109), Y132 (= Y132), R167 (= R167), F191 (≠ N191), L203 (= L203), Y206 (= Y206), H258 (= H258), R261 (= R261)
- binding flavin mononucleotide: Y26 (= Y25), Y27 (= Y26), A79 (≠ P78), T80 (≠ M79), A81 (= A80), S108 (= S107), Q130 (= Q130), Y132 (= Y132), T158 (= T158), K234 (= K234), H258 (= H258), G259 (= G259), R261 (= R261), D289 (= D289), G290 (= G290), R293 (= R293), G312 (= G312), R313 (= R313)
5qigA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1407672867
52% identity, 99% coverage: 5:359/359 of query aligns to 3:358/359 of 5qigA
- active site: Y129 (= Y132), D157 (= D160), H257 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), T155 (= T158), K233 (= K234), H257 (= H258), G258 (= G259), R260 (= R261), D288 (= D289), G289 (= G290), G290 (= G291), R292 (= R293), G311 (= G312), R312 (= R313)
- binding 2-cyclopropyl-1~{H}-imidazole-4-carboxamide: R223 (≠ Q224), G248 (= G249), F320 (≠ V321), K324 (≠ T325), D328 (≠ H329)
Sites not aligning to the query:
5qifA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z31792168
52% identity, 99% coverage: 5:359/359 of query aligns to 3:358/359 of 5qifA
- active site: Y129 (= Y132), D157 (= D160), H257 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), K233 (= K234), H257 (= H258), G258 (= G259), R260 (= R261), D288 (= D289), G289 (= G290), R292 (= R293), G311 (= G312), R312 (= R313)
- binding 2-cyclohexyl-~{N}-pyridin-3-yl-ethanamide: R223 (≠ Q224), R224 (≠ S225), S227 (= S228)
2rdwA Crystal structure of human glycolate oxidase in complex with sulfate (see paper)
52% identity, 99% coverage: 5:359/359 of query aligns to 3:358/359 of 2rdwA
- active site: S105 (= S107), Y129 (= Y132), T155 (= T158), D157 (= D160), K233 (= K234), H257 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), Q127 (= Q130), Y129 (= Y132), T155 (= T158), K233 (= K234), H257 (= H258), G258 (= G259), R260 (= R261), D288 (= D289), G289 (= G290), R292 (= R293), G311 (= G312), R312 (= R313)
- binding sulfate ion: Y23 (= Y25), W107 (≠ M109), R164 (= R167), H257 (= H258), R260 (= R261)
6gmbA Structure of human hydroxyacid oxidase 1 bound with fmn and glycolate
52% identity, 99% coverage: 5:359/359 of query aligns to 6:361/362 of 6gmbA
- active site: Y132 (= Y132), D160 (= D160), H260 (= H258)
- binding flavin mononucleotide: Y26 (= Y25), Y27 (= Y26), A79 (≠ P78), T80 (≠ M79), A81 (= A80), S108 (= S107), Q130 (= Q130), Y132 (= Y132), K236 (= K234), H260 (= H258), G261 (= G259), R263 (= R261), D291 (= D289), G292 (= G290), G293 (= G291), R295 (= R293), G314 (= G312), R315 (= R313)
- binding glycolic acid: Y26 (= Y25), W110 (≠ M109), Y132 (= Y132), R167 (= R167), H260 (= H258), R263 (= R261)
2rduA Crystal structure of human glycolate oxidase in complex with glyoxylate (see paper)
52% identity, 99% coverage: 5:359/359 of query aligns to 4:359/360 of 2rduA
- active site: S106 (= S107), Y130 (= Y132), T156 (= T158), D158 (= D160), K234 (= K234), H258 (= H258)
- binding flavin mononucleotide: Y24 (= Y25), Y25 (= Y26), A77 (≠ P78), T78 (≠ M79), A79 (= A80), S106 (= S107), Q128 (= Q130), Y130 (= Y132), T156 (= T158), K234 (= K234), H258 (= H258), G259 (= G259), R261 (= R261), D289 (= D289), G290 (= G290), G291 (= G291), R293 (= R293), G312 (= G312), R313 (= R313)
- binding glyoxylic acid: Y24 (= Y25), W108 (≠ M109), Y130 (= Y132), R165 (= R167), H258 (= H258), R261 (= R261)
Q9UJM8 2-Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GO; GOX; Glyoxylate oxidase; EC 1.1.3.15; EC 1.2.3.5 from Homo sapiens (Human) (see 2 papers)
52% identity, 99% coverage: 5:359/359 of query aligns to 6:361/370 of Q9UJM8
- Y26 (= Y25) binding glyoxylate
- ATA 79:81 (≠ PMA 78:80) binding FMN
- S108 (= S107) binding FMN
- Q130 (= Q130) binding FMN
- Y132 (= Y132) binding glyoxylate
- T158 (= T158) binding FMN
- R167 (= R167) binding glyoxylate
- K236 (= K234) binding FMN
- S258 (= S256) binding FMN
- H260 (= H258) binding glyoxylate
- R263 (= R261) binding glyoxylate
- DGGVR 291:295 (≠ DGGIR 289:293) binding FMN
- GR 314:315 (= GR 312:313) binding FMN
7r4nA Structure of human hydroxyacid oxidase 1 bound with 5-bromo-n-methyl- 1h-indazole-3-carboxamide
52% identity, 99% coverage: 5:359/359 of query aligns to 5:351/352 of 7r4nA
- binding 5-bromanyl-N-methyl-1H-indazole-3-carboxamide: Y25 (= Y25), A80 (= A80), M81 (≠ F81), W109 (≠ M109), Y131 (= Y132), R166 (= R167), M182 (≠ L183), H250 (= H258), R253 (= R261)
- binding flavin mononucleotide: Y25 (= Y25), Y26 (= Y26), A78 (≠ P78), T79 (≠ M79), A80 (= A80), S107 (= S107), W109 (≠ M109), Q129 (= Q130), Y131 (= Y132), T157 (= T158), K226 (= K234), H250 (= H258), G251 (= G259), R253 (= R261), D281 (= D289), G282 (= G290), R285 (= R293), G304 (= G312), R305 (= R313)
5qieA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z2856434894
52% identity, 99% coverage: 5:359/359 of query aligns to 3:352/353 of 5qieA
- active site: Y129 (= Y132), D157 (= D160), H251 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), K227 (= K234), H251 (= H258), G252 (= G259), R254 (= R261), D282 (= D289), G283 (= G290), R286 (= R293), G305 (= G312), R306 (= R313)
- binding ~{N},~{N}-dimethyl-4-[(propan-2-ylamino)methyl]aniline: I3 (= I5), D322 (≠ H329)
5qihA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z2697514548
52% identity, 99% coverage: 5:359/359 of query aligns to 3:343/344 of 5qihA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), K218 (= K234), H242 (= H258), G243 (= G259), R245 (= R261), D273 (= D289), G274 (= G290), R277 (= R293), G296 (= G312), R297 (= R313)
- binding 1-methylindazole-3-carboxamide: Y23 (= Y25), A78 (= A80), M79 (≠ F81), W107 (≠ M109), Y129 (= Y132), M180 (≠ L183), L187 (= L203), H242 (= H258)
5qidA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1787627869
52% identity, 99% coverage: 5:359/359 of query aligns to 3:343/344 of 5qidA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), K218 (= K234), H242 (= H258), G243 (= G259), R245 (= R261), D273 (= D289), G274 (= G290), R277 (= R293), G296 (= G312), R297 (= R313)
- binding 5-chloranyl-~{N}-methyl-~{N}-[[(3~{S})-oxolan-3-yl]methyl]pyrimidin-4-amine: T57 (≠ R59), E320 (≠ N336), R323 (≠ D339)
5qicA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z30620520
52% identity, 99% coverage: 5:359/359 of query aligns to 3:343/344 of 5qicA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), K218 (= K234), H242 (= H258), G243 (= G259), R245 (= R261), D273 (= D289), G274 (= G290), R277 (= R293), G296 (= G312), R297 (= R313)
- binding cyclopropyl-[4-(4-fluorophenyl)piperazin-1-yl]methanone: Y131 (≠ H134), K132 (≠ R135), V136 (≠ L139), M180 (≠ L183), F183 (≠ L186), Y190 (= Y206), K193 (≠ N209), A194 (≠ Q210)
5qibA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with fmopl000388a
52% identity, 99% coverage: 5:359/359 of query aligns to 3:343/344 of 5qibA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H258)
- binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: Y23 (= Y25), Y24 (= Y26), A76 (≠ P78), T77 (≠ M79), A78 (= A80), S105 (= S107), Q127 (= Q130), Y129 (= Y132), K218 (= K234), H242 (= H258), G243 (= G259), R245 (= R261), D273 (= D289), G274 (= G290), G275 (= G291), R277 (= R293), G296 (= G312), R297 (= R313)
- binding 5-(cyclohexylamino)pyrimidine-2,4(1H,3H)-dione: Y23 (= Y25), M79 (≠ F81), W107 (≠ M109), Y129 (= Y132), R164 (= R167), M180 (≠ L183), L187 (= L203), H242 (= H258), R245 (= R261)
7r4oA Structure of human hydroxyacid oxidase 1 bound with 2-((4h-1,2,4- triazol-3-yl)thio)-1-(4-(3-chlorophenyl)piperazin-1-yl)ethan-1-one
51% identity, 99% coverage: 5:359/359 of query aligns to 4:340/341 of 7r4oA
- binding flavin mononucleotide: Y25 (= Y26), A77 (≠ P78), T78 (≠ M79), A79 (= A80), S106 (= S107), Q128 (= Q130), Y130 (= Y132), K215 (= K234), H239 (= H258), G240 (= G259), R242 (= R261), D270 (= D289), G271 (= G290), R274 (= R293), G293 (= G312), R294 (= R313)
- binding 2-((4H-1,2,4-triazol-3-yl)thio)-1-(4-(3-chlorophenyl)piperazin-1-yl)ethan-1-one: Y132 (≠ H134), K133 (≠ R135), D134 (= D136), N183 (= N185), K190 (≠ N209)
2w0uA Crystal structure of human glycolate oxidase in complex with the inhibitor 5-[(4-chlorophenyl)sulfanyl]- 1,2,3-thiadiazole-4- carboxylate. (see paper)
51% identity, 99% coverage: 5:359/359 of query aligns to 2:332/334 of 2w0uA
- active site: S104 (= S107), Y128 (= Y132), T154 (= T158), D156 (= D160), K207 (= K234), H231 (= H258)
- binding 5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole-4-carboxylate: Y22 (= Y25), M78 (≠ F81), W106 (≠ M109), Y128 (= Y132), R163 (= R167), L176 (= L203), Y179 (= Y206), V180 (= V207), H231 (= H258), R234 (= R261)
- binding flavin mononucleotide: Y23 (= Y26), A75 (≠ P78), T76 (≠ M79), A77 (= A80), S104 (= S107), Q126 (= Q130), T154 (= T158), K207 (= K234), H231 (= H258), G232 (= G259), R234 (= R261), D262 (= D289), G263 (= G290), R266 (= R293), G285 (= G312), R286 (= R313)
7r4pA Structure of human hydroxyacid oxidase 1 bound with 6-amino-1-benzyl- 5-(methylamino)pyrimidine-2,4(1h,3h)-dione
51% identity, 99% coverage: 5:359/359 of query aligns to 4:336/337 of 7r4pA
- binding flavin mononucleotide: Y25 (= Y26), A77 (≠ P78), T78 (≠ M79), A79 (= A80), S106 (= S107), Q128 (= Q130), Y130 (= Y132), T156 (= T158), K211 (= K234), H235 (= H258), G236 (= G259), R238 (= R261), D266 (= D289), G267 (= G290), R270 (= R293), G289 (= G312), R290 (= R313)
- binding 6-amino-1-benzyl-5-(methylamino)pyrimidine-2,4(1H,3H)-dione: Y24 (= Y25), A79 (= A80), M80 (≠ F81), W108 (≠ M109), Y130 (= Y132), R165 (= R167), L180 (= L203), H235 (= H258), R238 (= R261)
6w4cA Crystal structure of hao1 in complex with indazole acid inhibitor - compound 5 (see paper)
50% identity, 100% coverage: 1:359/359 of query aligns to 1:335/336 of 6w4cA
- active site: Y131 (= Y132), D159 (= D160), H234 (= H258)
- binding flavin mononucleotide: Y25 (= Y25), Y26 (= Y26), A78 (≠ P78), T79 (≠ M79), A80 (= A80), S107 (= S107), Q129 (= Q130), Y131 (= Y132), T157 (= T158), K210 (= K234), H234 (= H258), G235 (= G259), R237 (= R261), D265 (= D289), G266 (= G290), R269 (= R293), G288 (= G312), R289 (= R313)
- binding 5-[[3-[3-(dimethylamino)-1,2,4-oxadiazol-5-yl]-2-oxidanyl-phenyl]methylamino]-2~{H}-indazole-3-carboxylic acid: Y25 (= Y25), A80 (= A80), M81 (≠ F81), M84 (≠ L84), W109 (≠ M109), Y131 (= Y132), R166 (= R167), A180 (≠ F204), V183 (= V207), H234 (= H258), R237 (= R261)
P05414 Glycolate oxidase; GAO; GOX; Short chain alpha-hydroxy acid oxidase; EC 1.1.3.15 from Spinacia oleracea (Spinach) (see 4 papers)
51% identity, 98% coverage: 6:356/359 of query aligns to 5:352/369 of P05414
- Y24 (= Y25) mutation to F: 10-fold decrease in affinity for glycolate.
- PTA 77:79 (≠ PMA 78:80) binding FMN
- S106 (= S107) binding FMN
- W108 (≠ M109) mutation to S: 100-fold decrease in affinity for glycolate and 500-fold decrease in activity.
- QLY 127:129 (= QLY 130:132) binding FMN
- T155 (= T158) binding FMN
- K230 (= K234) binding FMN
- S252 (= S256) binding FMN
- DGGVR 285:289 (≠ DGGIR 289:293) binding FMN
- GR 308:309 (= GR 312:313) binding FMN
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
5zbmB Structure of glycolate oxidase containing fmn from nicotiana benthamiana (see paper)
51% identity, 97% coverage: 6:354/359 of query aligns to 4:344/353 of 5zbmB
- active site: Y128 (= Y132), D156 (= D160), H248 (= H258)
- binding flavin mononucleotide: Y23 (= Y25), Y24 (= Y26), P76 (= P78), T77 (≠ M79), W107 (≠ M109), Q126 (= Q130), Y128 (= Y132), T154 (= T158), K224 (= K234), H248 (= H258), G249 (= G259), R251 (= R261), D279 (= D289), G280 (= G290), R283 (= R293), G302 (= G312), R303 (= R313)
Query Sequence
>WP_011611983.1 NCBI__GCF_000014265.1:WP_011611983.1
MNKPINIFEYESLAPKYLSQMALDYYASGAWDEVTLRDNRTAYEKYKLRPRMLVDVSQRN
LSTKILGQLMKMPILIAPMAFQCLAHPEGELATARVAADHGITMVLSTMSTKSLEDVALA
TNVPQSLWFQLYVHRDRFLTRTLVERAKAAGYQALCLTVDAPVLGVRERDRRNQFTLPSG
LELANLTSMANLEIPETEEESGLFAYVANQFDPALTWQDLEWLQSLTSLPVIVKGILRGD
DAVRAVEHGAKGIIVSNHGGRQLDGAIATIDALPEVVAAVGNKVDVLMDGGIRRGTDILK
ALALGAKAVLIGRPVLWALAVNGETGVHHLLELLRNELDVAMALSGCAKVENINPSLVR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory