SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011613632.1 NCBI__GCF_000014265.1:WP_011613632.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8xd5A Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction (see paper)
53% identity, 97% coverage: 6:420/428 of query aligns to 7:415/419 of 8xd5A

query
sites
8xd5A

F

F

D

E

D

M

A

A

S

V

R

K

R

Q

L

L

E

E

R

R

A

A

L

A

Q

Q

Y

Y

V

M

S

D

L

I

S

S

E

E

D

E

T

A

K

L

E

E

R

W

L

L

K

K

Y

K

P

P

K

M

A

R

T

I

L

V

I

E

V

V

S

S

I

V

P

P

V

I

R

E

M

M

D

D

G

G

S

S

L

V

R

K

V

V

F

F

Q

T

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

N

D

W

T

A

R

R

G

G

P

P

T

T

K
|
K

G

G

I

I

R
|
R

Y

W

H

H

P

P

N

A

V

E

S

T

I

L

D

S

E

T

V

V

K

K

S

A

L

L

A

A

F

T

W

W

M
|
M

T

T

F

W

K

K

C

V

A

A

V

V

S

D

L

L

P

P

F

Y

G

G

A

G

K
|
K

G

G

I

I

T

I

V

V

N

N

P

P

K

K

E

E

L

L

S

S

R

E

M

R

E

E

L

Q

E

E

R

R

L

L

S

A

R

R

G

A

Y

Y

I

I

D

R

A

A

I

V

A

Y

D

D

F

V

I

I

G

G

P

P

D

W

T

T

D

D

I

I

P

P

A
|
A

P

P

D
|
D

M
x
V

Y
|
Y

T

T

N

N

P

P

M

K

I

I

M

M

G

G

W

W

M

M

D

D

E

E

Y

Y

S

E

I

T

I

I

R

M

R

R

Q

R

L

K

S

G

P

P

A

A

-

F

-

G

V

V

I

I

T

T

G

G

K

K

P

P

V

L

S

S

I

I

G

G

S

S

L

L

G

G

R

R

N

G

T

T

A

A

T
|
T

A

A

M

Q

G

G

A

A

F

I

F

F

A

T

I

I

E

R

T

E

I

A

M

A

P

K

K

A

F

L

E

G

Y

I

T

D

P

L

E

K

K

G

T

K

V

I

A

A

I

V

Q

Q

G

G

F
x
Y

G
|
G

N
|
N

A
|
A

G

G

A

Y

F

Y

L

T

A

A

E

K

L

L

L

A

C

K

L

E

H

Q

-

L

G

G

Y

M

K

T

V

V

A

A

V

V

S

S

D
|
D

S
|
S

Q

R

G

G

I

I

Y

Y

S

N

A

P

Q

D

G

G

L

L

D

D

I

P

P

D

S

E

I

V

R

L

Q

K

Y

W

K
|
K

E

R

V

E

N

H

K

-

Q

-

I

-

Q

-

A

-

V

-

Y

-

C

-

K

-

D

G

S

S

V

V

C

K

N

D

I

F

V

P

E

G

H

A

T

T

V

N

L

I

T

T

N
|
N

E

E

L

L

A

E

L

L

D

E

V

V

D

D

I

V

L

L

I

A

P

P

A
|
A

L
x
I

E

E

N

E

Q

V

I

I

T

T

E

E

E

K

N

N

V

A

K

D

D

N

I

I

Q

K

A

A

K

K

F

I

I

V

F

A

E

E

A

V

A

A

N
|
N

G

G

P

P

T

V

T

T

S

P

G

E

A

A

D

D

K

D

I

I

L

L

E

R

D

E

R

K

G

G

V

I

Y

L

V

Q

F

I

P

P

D

D

I

F

L

L

I

C

N
|
N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

N

G

G

L

Y

Y

Y

W

W

T

T

L

E

D

E

E

E

V

V

N

R

Q

E

K

K

L

L

K

D

A

K

K

K

M

M

V

T

A

K

E

A

T

F

E

W

N

E

I

V

W

Y

Q

N

I

T

S

H

Q

K

E

D

L

K

S

N

I

I

S

H

M

M

R

R

T

D

A

A

Y

Y

V

V

H

V

G

A

L

V

D

S

R

R

L

V

G

Y

Q

Q

A

A

I

M

N

K

A

D

K

R

G

G

binding gamma-l-glutamic acid: K69 (= K68), M90 (= M89), K105 (= K104), A143 (= A142), D145 (= D144), S351 (= S356)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R73 (= R72), D145 (= D144), V146 (≠ M145), Y147 (= Y146), T191 (= T188), Y220 (≠ F217), G221 (= G218), N222 (= N219), A223 (= A220), D244 (= D240), S245 (= S241), K264 (= K260), N281 (= N286), A295 (= A300), A296 (= A301), I297 (≠ L302), N319 (= N324), N344 (= N349)

8xcsA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADPH, akg and nh4 in the initial stage of reaction (see paper)
53% identity, 97% coverage: 6:420/428 of query aligns to 6:414/418 of 8xcsA

query
sites
8xcsA

F

F

D

E

D

M

A

A

S

V

R

K

R

Q

L

L

E

E

R

R

A

A

L

A

Q

Q

Y

Y

V

M

S

D

L

I

S

S

E

E

D

E

T

A

K

L

E

E

R

W

L

L

K

K

Y

K

P

P

K

M

A

R

T

I

L

V

I

E

V

V

S

S

I

V

P

P

V

I

R

E

M

M

D

D

G

G

S

S

L

V

R

K

V

V

F

F

Q

T

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

N

D

W

T

A

R

R

G

G

P

P

T

T

K
|
K

G

G

G
|
G

I

I

R
|
R

Y

W

H

H

P

P

N

A

V

E

S

T

I

L

D

S

E

T

V

V

K

K

S

A

L

L

A

A

F

T

W

W

M
|
M

T

T

F

W

K
|
K

C

V

A

A

V

V

S

D

L

L

P

P

F

Y

G

G

A

G

K
|
K

G

G

I

I

T

I

V

V

N

N

P

P

K
|
K

E

E

L

L

S

S

R

E

M

R

E

E

L

Q

E

E

R

R

L

L

S

A

R

R

G

A

Y

Y

I

I

D

R

A

A

I

V

A

Y

D

D

F

V

I

I

G

G

P

P

D

W

T

T

D

D

I

I

P

P

A
|
A

P
|
P

D
|
D

M
x
V

Y
|
Y

T

T

N

N

P

P

M

K

I

I

M

M

G

G

W

W

M

M

D

D

E

E

Y

Y

S

E

I

T

I

I

R

M

R

R

Q

R

L

K

S

G

P

P

A

A

-

F

-

G

V

V

I

I

T

T

G

G

K

K

P

P

V

L

S

S

I

I

G

G

S

S

L

L

G

G

R

R

N

G

T

T

A

A

T
|
T

A

A

M

Q

G

G

A

A

F

I

F

F

A

T

I

I

E

R

T

E

I

A

M

A

P

K

K

A

F

L

E

G

Y

I

T

D

P

L

E

K

K

G

T

K

V

I

A

A

I

V

Q

Q

G

G

F
x
Y

G
|
G

N
|
N

A
|
A

G

G

A

Y

F

Y

L

T

A

A

E

K

L

L

L

A

C

K

L

E

H

Q

-

L

G

G

Y

M

K

T

V

V

A

A

V

V

S

S

D
|
D

S
|
S

Q

R

G

G

I

I

Y

Y

S

N

A

P

Q

D

G

G

L

L

D

D

I

P

P

D

S

E

I

V

R

L

Q

K

Y

W

K
|
K

E

R

V

E

N

H

K

-

Q

-

I

-

Q

-

A

-

V

-

Y

-

C

-

K

-

D

G

S

S

V

V

C

K

N

D

I

F

V

P

E

G

H

A

T

T

V

N

L

I

T

T

N

N

E

E

L

L

A

E

L

L

D

E

V

V

D

D

I

V

L

L

I

A

P

P

A

A

A
|
A

L
x
I

E

E

N

E

Q

V

I

I

T

T

E

E

E

K

N

N

V

A

K

D

D

N

I

I

Q

K

A

A

K

K

F

I

I

V

F

A

E

E

A

V

A

A

N
|
N

G

G

P

P

T

V

T

T

S

P

G

E

A

A

D

D

K

D

I

I

L

L

E

R

D

E

R

K

G

G

V

I

Y

L

V

Q

F

I

P

P

D

D

I

F

L

L

I

C

N

N

A

A

G

G

G
|
G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

N

G

G

L

Y

Y

Y

W

W

T

T

L

E

D

E

E

E

V

V

N

R

Q

E

K

K

L

L

K

D

A

K

K

K

M

M

V

T

A

K

E

A

T

F

E

W

N

E

I

V

W

Y

Q

N

I

T

S

H

Q

K

E

D

L

K

S

N

I

I

S

H

M

M

R

R

T

D

A

A

Y

Y

V

V

H

V

G

A

L

V

D

S

R

R

L

V

G

Y

Q

Q

A

A

I

M

N

K

A

D

K

R

G

G

binding 2-oxoglutaric acid: K68 (= K68), G70 (= G70), M89 (= M89), K92 (= K92), K104 (= K104), A142 (= A142), D144 (= D144), G346 (= G352), S350 (= S356)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R72 (= R72), K112 (= K112), P143 (= P143), D144 (= D144), V145 (≠ M145), Y146 (= Y146), T190 (= T188), Y219 (≠ F217), G220 (= G218), N221 (= N219), A222 (= A220), D243 (= D240), S244 (= S241), K263 (= K260), A295 (= A301), I296 (≠ L302), N318 (= N324)

8xcoA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus incorporating NADPH in the initial stage of reaction (see paper)
53% identity, 97% coverage: 6:420/428 of query aligns to 4:412/416 of 8xcoA

query
sites
8xcoA

F

F

D

E

D

M

A

A

S

V

R

K

R

Q

L

L

E

E

R

R

A

A

L

A

Q

Q

Y

Y

V

M

S

D

L

I

S

S

E

E

D

E

T

A

K

L

E

E

R

W

L

L

K

K

Y

K

P

P

K

M

A

R

T

I

L

V

I

E

V

V

S

S

I

V

P

P

V

I

R

E

M

M

D

D

G

G

S

S

L

V

R

K

V

V

F

F

Q

T

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

N

D

W

T

A

R

R

G

G

P

P

T

T

K

K

G

G

I

I

R

R

Y

W

H

H

P

P

N

A

V

E

S

T

I

L

D

S

E

T

V

V

K

K

S

A

L

L

A

A

F

T

W

W

M

M

T

T

F

W

K

K

C

V

A

A

V

V

S

D

L

L

P

P

F

Y

G

G

A

G

K

K

G

G

I

I

T

I

V

V

N

N

P

P

K

K

E

E

L

L

S

S

R

E

M

R

E

E

L

Q

E

E

R

R

L

L

S

A

R

R

G

A

Y

Y

I

I

D

R

A

A

I

V

A

Y

D

D

F

V

I

I

G

G

P

P

D

W

T

T

D

D

I

I

P

P

A

A

P

P

D

D

M

V

Y

Y

T

T

N

N

P

P

M

K

I

I

M

M

G

G

W

W

M

M

D

D

E

E

Y

Y

S

E

I

T

I

I

R

M

R

R

Q

R

L

K

S

G

P

P

A

A

-

F

-

G

V

V

I

I

T

T

G

G

K

K

P

P

V

L

S

S

I

I

G

G

S

S

L

L

G

G

R

R

N

G

T

T

A

A

T
|
T

A

A

M

Q

G

G

A

A

F

I

F

F

A

T

I

I

E

R

T

E

I

A

M

A

P

K

K

A

F

L

E

G

Y

I

T

D

P

L

E

K

K

G

T

K

V

I

A

A

I

V

Q

Q

G

G

F
x
Y

G
|
G

N
|
N

A
|
A

G

G

A

Y

F

Y

L

T

A

A

E

K

L

L

L

A

C

K

L

E

H

Q

-

L

G

G

Y

M

K

T

V

V

A

A

V

V

S

S

D
|
D

S
|
S

Q

R

G

G

I

I

Y

Y

S

N

A

P

Q

D

G

G

L

L

D

D

I

P

P

D

S

E

I

V

R

L

Q

K

Y

W

K
|
K

E

R

V

E

N

H

K

-

Q

-

I

-

Q

-

A

-

V

-

Y

-

C

-

K

-

D

G

S

S

V

V

C

K

N

D

I

F

V

P

E

G

H

A

T

T

V

N

L

I

T

T

N

N

E

E

L

L

A

E

L

L

D

E

V

V

D

D

I

V

L

L

I

A

P

P

A

A

L
x
I

E

E

N

E

Q

V

I

I

T

T

E

E

E

K

N

N

V

A

K

D

D

N

I

I

Q

K

A

A

K

K

F

I

I

V

F

A

E

E

A

V

A

A

N

N

G

G

P

P

T

V

T

T

S

P

G

E

A

A

D

D

K

D

I

I

L

L

E

R

D

E

R

K

G

G

V

I

Y

L

V

Q

F

I

P

P

D

D

I

F

L

L

I

C

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

N

G

G

L

Y

Y

Y

W

W

T

T

L

E

D

E

E

E

V

V

N

R

Q

E

K

K

L

L

K

D

A

K

K

K

M

M

V

T

A

K

E

A

T

F

E

W

N

E

I

V

W

Y

Q

N

I

T

S

H

Q

K

E

D

L

K

S

N

I

I

S

H

M

M

R

R

T

D

A

A

Y

Y

V

V

H

V

G

A

L

V

D

S

R

R

L

V

G

Y

Q

Q

A

A

I

M

N

K

A

D

K

R

G

G

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T188 (= T188), Y217 (≠ F217), G218 (= G218), N219 (= N219), A220 (= A220), D241 (= D240), S242 (= S241), K261 (= K260), I294 (≠ L302)

8zneA Cryo-em structure of w89f mutated glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction
53% identity, 97% coverage: 6:420/428 of query aligns to 4:412/416 of 8zneA

query
sites
8zneA

F

F

D

E

D

M

A

A

S

V

R

K

R

Q

L

L

E

E

R

R

A

A

L

A

Q

Q

Y

Y

V

M

S

D

L

I

S

S

E

E

D

E

T

A

K

L

E

E

R

W

L

L

K

K

Y

K

P

P

K

M

A

R

T

I

L

V

I

E

V

V

S

S

I

V

P

P

V

I

R

E

M

M

D

D

G

G

S

S

L

V

R

K

V

V

F

F

Q

T

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

N

D

W

T

A

R

R

G

G

P

P

T

T

K

K

G

G

I

I

R

R

Y

W

H

H

P

P

N

A

V

E

S

T

I

L

D

S

E

T

V

V

K

K

S

A

L

L

A

A

F

T

W

F

M
|
M

T

T

F

W

K
|
K

C

V

A

A

V

V

S

D

L

L

P

P

F

Y

G

G

A

G

K

K

G

G

I

I

T

I

V

V

N

N

P

P

K

K

E

E

L

L

S

S

R

E

M

R

E

E

L

Q

E

E

R

R

L

L

S

A

R

R

G

A

Y

Y

I

I

D

R

A

A

I

V

A

Y

D

D

F

V

I

I

G

G

P

P

D

W

T

T

D

D

I

I

P

P

A
|
A

P

P

D
|
D

M

V

Y

Y

T

T

N

N

P

P

M

K

I

I

M

M

G

G

W

W

M

M

D

D

E

E

Y

Y

S

E

I

T

I

I

R

M

R

R

Q

R

L

K

S

G

P

P

A

A

-

F

-

G

V

V

I

I

T

T

G

G

K

K

P

P

V

L

S

S

I

I

G

G

S

S

L

L

G

G

R

R

N

G

T

T

A

A

T

T

A

A

M

Q

G

G

A

A

F

I

F

F

A

T

I

I

E

R

T

E

I

A

M

A

P

K

K

A

F

L

E

G

Y

I

T

D

P

L

E

K

K

G

T

K

V

I

A

A

I

V

Q

Q

G
|
G

F
x
Y

G
|
G

N
|
N

A
|
A

G

G

A

Y

F

Y

L

T

A

A

E

K

L

L

L

A

C

K

L

E

H

Q

-

L

G

G

Y

M

K

T

V

V

A

A

V

V

S

S

D
|
D

S
|
S

Q

R

G

G

I

I

Y

Y

S

N

A

P

Q

D

G

G

L

L

D

D

I

P

P

D

S

E

I

V

R

L

Q

K

Y

W

K

K

E

R

V

E

N

H

K

-

Q

-

I

-

Q

-

A

-

V

-

Y

-

C

-

K

-

D

G

S

S

V

V

C

K

N

D

I

F

V

P

E

G

H

A

T

T

V

N

L

I

T

T

N

N

E

E

L

L

A

E

L

L

D

E

V

V

D

D

I

V

L

L

I

A

P

P

A

A

A
|
A

L
x
I

E

E

N

E

Q

V

I

I

T

T

E

E

E

K

N

N

V

A

K

D

D

N

I

I

Q

K

A

A

K

K

F

I

I

V

F

A

E

E

A

V

A

A

N

N

G

G

P

P

T

V

T

T

S

P

G

E

A

A

D

D

K

D

I

I

L

L

E

R

D

E

R

K

G

G

V

I

Y

L

V

Q

F

I

P

P

D

D

I

F

L

L

I

C

N

N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

N

G

G

L

Y

Y

Y

W

W

T

T

L

E

D

E

E

E

V

V

N

R

Q

E

K

K

L

L

K

D

A

K

K

K

M

M

V

T

A

K

E

A

T

F

E

W

N

E

I

V

W

Y

Q

N

I

T

S

H

Q

K

E

D

L

K

S

N

I

I

S

H

M

M

R

R

T

D

A

A

Y

Y

V

V

H

V

G

A

L

V

D

S

R

R

L

V

G

Y

Q

Q

A

A

I

M

N

K

A

D

K

R

G

G

binding glutamic acid: M87 (= M89), K90 (= K92), A140 (= A142), D142 (= D144), S348 (= S356)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G216 (= G216), Y217 (≠ F217), G218 (= G218), N219 (= N219), A220 (= A220), D241 (= D240), S242 (= S241), A293 (= A301), I294 (≠ L302)

P39633 Catabolic NAD-specific glutamate dehydrogenase RocG; NAD-GDH; Glutamate dehydrogenase; GlutDH; Trigger enzyme RocG; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see 2 papers)
49% identity, 97% coverage: 4:420/428 of query aligns to 16:422/424 of P39633

query
sites
P39633

S

N

L

L

F

F

D

L

D

S

A

T

S

Q

R

T

R

I

L

I

E

K

R
x
E

A

A

L

L

Q

R

Y

K

V

L

S

G

L

Y

S

P

E

G

D

D

T

M

K

Y

E

E

R

L

L

M

K

K

Y

E

P

P

K

Q

A

R

T

M

L

L

I

T

V

V

S

R

I

I

P

P

V

V

R

K

M

M

D

D

D

N

G

G

S

S

L

V

R

K

V

V

F

F

Q

T

G

G

Y

Y

R

R

V

S

R

Q

Y

H

D

N

D

D

T

A

R

V

G

G

P

P

T

T

K

K

G

G

I

V

R

R

Y

F

H

H

P

P

N

E

V

V

S

N

I

E

D

E

E
|
E

V

V

K

K

S

A

L

L

A

S

F

I

W

W

M

M

T

T

F

L

K

K

C

C

A

G

V

I

V

A

S

N

L

L

P

P

F

Y

G

G

A

G

K

K

G

G

I

I

T

I

V

C

N
x
D

P

P

K

R

E

T

L

M

S

S

R

F

M

G

E

E

L

L

E

E

R

R

L

L

S

S

R

R

G

G

Y

Y

I

V

D

R

A

A

I

I

A

S

D
x
Q

F

I

I

V

G

G

P

P

D

T

T

K

D

D

I

I

P

P

A

A

P

P

D

D

M

V

Y
|
Y

T

T

N

N

P

S

M

Q

I

I

M

M

G

A

W

W

M

M

D

D

E

E

Y

Y

S

S

I

R

I

L

R

R

R

E

Q

F

L

D

S

S

P

P

A

G

V

F

I

I

T

T

G

G

K

K

P

P

V

L

S

V

I

L

G

G

S

S

L

Q

G

G

R

R

N

E

T

T

A

A

T

T

A

A

M

Q

G

G

A

V

F

T

F

I

A

C

I

I

E

E

T

E

I

A

M

V

P

K

K

K

F

K

E

G

Y

I

T

K

P

L

E

Q

K

N

T

A

T

R

V

I

A

I

I

I

Q

Q

G

G

F

F

G

G

N

N

A

A

G

G

A
x
S

F

F

L

L

A

A

E

K

L

F

L

M

C

H

L

D

H

A

G

G

Y

A

K

K

V

V

V

I

A

G

V

I

S

S

D

D

S

A

Q

N

G

G

I

L

Y

Y

S

N

A

P

Q

D

G

G

L

L

D

D

I

I

P

P

S

Y

I

L

R

L

Q

D

Y

K

K

R

E

D

V

S

N

F

K

G

Q

M

I

V

Q

T

A

N

V

L

Y

F

C

T

K

D

D

-

S

-

V

-

C

-

N

-

I

-

V

-

E

-

H

-

T

-

V

V

L

I

T

T

N

N

E

E

L

L

A

E

L

K

D

D

V

C

D

D

I

I

L

L

I

V

P

P

A

A

L

I

E

S

N

N

Q

Q

I

I

T

T

E

A

E

K

N

N

V

A

K

H

D

N

I

I

Q

Q

A

A

K

S

F

I

I

V

F

V

E

E

A
|
A

A

A

N

N

G

G

P

P

T

T

S

I

G

D

A

A

D

T

K

K

I

I

L

L

E

N

D

E

R

R

G

G

V

V

Y

L

V

L

F

V

P

P

D

D

I

I

L

L

I

A

N

S

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

N

S

Q

G

G

L

Y

Y

Y

W

W

T

S

L

E

D

E

E

E

V

V

N

A

Q

E

K

K

L

L

K

R

A

S

K

V

M

M

V

V

A

S

E

S

T

F

E

E

N

T

I

I

W

Y

Q

Q

I

T

S

A

Q

A

E

T

L

H

S

K

I

V

S

D

M

M

R

R

T

L

A

A

Y

Y

V

M

H

T

G

G

L

I

D

R

R

K

L

S

G

A

Q

E

A

A

I

S

N

R

A

F

K

R

G

G

E27 (≠ R15) mutation to F: Increase of thermostability 8 degrees Celsius higher than that of the wild-type.
E93 (= E81) mutation to K: Reduces the affinity for glutamate and ammonium.
D122 (≠ N110) mutation to N: Unable to control gltAB expression via an inhibitory interactions with the transcriptional regulator GltC. Reduces the affinity for glutamate and ammonium.
Q144 (≠ D132) mutation to R: Increase of thermostability 20 degrees Celsius higher than that of the wild-type.
Y158 (= Y146) mutation to H: Reduces the affinity for glutamate and ammonium.
S234 (≠ A222) mutation to R: Reduces the affinity for glutamate and ammonium.
A324 (= A322) mutation to R: No effect.

P50735 Cryptic catabolic NAD-specific glutamate dehydrogenase GudB; NAD-GDH; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see paper)
48% identity, 95% coverage: 13:420/428 of query aligns to 25:425/427 of P50735

query
sites
P50735

L

I

E

H

R

K

A

A

L

L

Q

E

Y

K

V

L

S

G

L

Y

S

P

E

E

D

E

T

V

K

Y

E

E

R

L

L

L

K

K

Y

E

P

P

K

M

A

R

T

L

L

L

I

T

V

V

S

K

I

I

P

P

V

V

R

R

M

M

D

D

G

G

S

S

L

V

R

K

V

I

F

F

Q

T

G

G

Y

Y

R

R

V

A

R

Q

Y

H

D

N

D

D

T

S

R

V

G

G

P

P

T

T

K

K

G

G

I

I

R

R

Y

F

H

H

P

P

N

N

V

V

S

T

I

E

D

K

E

E

V

V

K

K

S

A

-
x
V

-
x
K

-
x
A

L

L

A

S

F

I

W

W

M

M

T

S

F

L

K

K

C

C

A

G

V

I

S

D

L

L

P

P

F

Y

G

G

A

G

K

K

G

G

I

I

T

V

V

C

N

D

P

P

K

R

E

D

L

M

S

S

R

F

M

R

E

E

L

L

E

E

R

R

L

L

S

S

R

R

G

G

Y

Y

I

V

D

R

A

A

I

I

A

S

D

Q

F

I

I

V

G

G

P

P

D

T

T

K

D

D

I

V

P

P

A

A

P

P

D

D

M

V

Y

F

T

T

N

N

P

S

M

Q

I

I

M

M

G

A

W

W

M

M

D

D

E

E

Y

Y

S

S

I

R

I

I

R

D

R

E

Q

F

L

N

S

S

P

P

A

G

V

F

I

I

T

T

G

G

K

K

P

P

V

L

S

V

I

L

G

G

S

S

L

H

G

G

R

R

N

E

T

S

A

A

T

T

A

A

M

K

G

G

A

V

F

T

F

I

A

C

I

I

E

K

T

E

I

A

M

A

P

K

K

K

F

R

E

G

Y

I

T

D

P

I

E

K

K

G

T

A

T

R

V

V

A

V

I

V

Q

Q

G

G

F

F

G

G

N

N

A

A

G

G

A

S

F

Y

L

L

A

A

E

K

L

F

L

M

C

H

L

D

H

A

G

G

Y

A

K

K

V

V

A

G

V

I

S

S

D

D

S

A

Q

Y

G

G

I

L

Y

Y

S

D

A

P

Q

E

G

G

L

L

D

D

I

I

P

D

S

Y

I

L

R

L

Q

D

Y

R

K

R

E

D

V

S

N

F

K

G

Q

T

I

V

Q

T

A

K

V

L

Y

F

C

-

K

-

D

-

S

-

V

-

C

-

N

-

I

-

V

-

E

-

H

N

T

D

V

T

L

I

T

T

N

N

E

Q

E

E

L

L

A

E

L

L

D

D

V

C

D

D

I

I

L

L

I

V

P

P

A

A

L

I

E

E

N

N

Q

Q

I

I

T

T

E

E

N

N

V

A

K

H

D

N

I

I

Q

R

A

A

K

K

F

I

I

V

F

V

E

E

A

A

N

N

G

G

P

P

T

T

S

L

G

E

A

G

D

T

K

K

I

I

L

L

E

S

D

D

R

R

G

D

V

I

Y

L

V

L

F

V

P

P

D

D

I

V

L

L

I

A

N

S

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

N

S

Q

G

G

L

F

Y

Y

W

W

T

S

L

E

D

E

E

E

V

V

N

E

Q

E

K

K

L

L

K

E

A

K

K

M

M

M

V

V

A

K

E

S

T

F

E

N

N

N

I

I

W

Y

Q

E

I

M

S

A

Q

N

E

N

L

R

S

R

I

I

S

D

M

M

R

R

T

L

A

A

Y

Y

V

M

H

V

G

G

L

V

D

R

R

K

L

M

G

A

Q

E

A

A

I

S

N

R

A

F

K

R

G

G

VKA 97:99 (vs. gap) mutation Missing: In gudB1; gains glutamate dehydrogenase activity, restores growth on proline, arginine, ornithine.

4xgiA Crystal structure of glutamate dehydrogenase from burkholderia thailandensis
44% identity, 97% coverage: 11:425/428 of query aligns to 19:415/416 of 4xgiA

query
sites
4xgiA

R

Q

L

V

E

D

R

R

A

V

L

A

Q

P

Y

Y

V

L

S

G

L

S

S

L

E

S

D

R

T

W

K

I

E

E

R

T

L

L

K

K

Y

R

P

P

K

K

A

R

T

I

L

L

I

I

V

V

S

D

I

V

P

P

V

I

R

E

M

L

D

D

D

N

G

G

S

T

L

V

R

A

V

H

F

F

Q

E

G

G

Y

Y

R

R

V

V

R

Q

Y

H

D

N

D

V

T

S

R

R

G

G

P

P

T

G

K
|
K

G

G

G
|
G

I

V

R
|
R

Y

Y

H

H

P

Q

N

D

V

V

S

T

I

L

D

S

E

E

V

V

K

M

S

A

L

L

A

S

F

A

W

W

M
|
M

T

S

F

V

K
|
K

C

N

A

A

V

A

V

V

S

N

L

V

P

P

F

Y

G

G

A

A

K
|
K

G

G

I

I

T

R

V

V

N

D

P

P

K

R

E

K

L

L

S

S

R

R

M

G

E

E

L

L

E

E

R

R

L

V

S

T

R

R

G

R

Y

Y

I

T

D

S

A

E

I

I

A

G

D

I

F

I

I

I

G

G

P

P

D

N

T

T

D

D

I

I

P

P

A
|
A

P

P

D
|
D

M
x
V

Y

N

T

T

N

N

P

E

M

Q

I

I

M

M

G

A

W

W

M

M

D

D

E

T

Y

Y

S

S

I

M

I

N

R

Q

R

G

Q

Q

L

T

S

A

P

T

A

G

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

S

I

L

G

G

S

S

L

L

G

G

R
|
R

N

K

T

E

A

A

T
|
T

A

G

M

R

G

G

A

V

F

F

F

V

A

V

I

G

E

C

T

E

I

A

M

A

P

K

K

K

F

K

E

G

Y

V

T

E

P

I

E

E

K

G

T

A

T

R

V

I

A

A

I

V

Q

Q

G
|
G

F

F

G
|
G

N
|
N

A
x
V

G

G

A

G

F

I

L

A

A

A

E

K

L

L

L

F

C

Q

L

E

H

A

G

G

Y

A

K

K

V

V

V

I

A

A

V

V

S

Q

D
|
D

S
x
H

Q

T

G

G

G

T

I

I

Y

H

S

Q

A

P

Q

A

G

G

L

V

D

D

I

-

P

-

S

-

I

-

R

-

Q

-

Y

-

K

-

E

-

V

T

N

A

K

K

Q

L

I

L

Q

D

A

H

V

V

Y

G

C

R

K

T

D

G

S

G

V

V

C

A

N

G

I

F

V

E

E

G

H

A

T

E

V

P

L

M

T

P

N

N

E

D

E

E

L

F

L

W

A

T

L

V

D

E

V

T

D

E

I

I

L

L

I

I

P

P

A
|
A

L

L

E

E

N

N

Q

Q

I

I

T

T

E

E

E

K

N

N

V

A

K

S

D

K

I

I

Q

R

A

T

K

K

F

I

I

I

F

V

E

E

A

G

A
|
A

N
|
N

G

G

P

P

T

T

S

T

G

A

A

A

D

D

K

D

I

I

L

L

E

S

D

A

R

N

G

G

V

V

Y

L

V

V

F

I

P

P

D

D

I

V

L

I

I

A

N
|
N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

-

R

-

S

-

G

-

L

-

Y

-

W

W

T

T

L

E

D

D

E

E

V

I

N

N

Q

H

K

R

L

L

K

E

A

R

K

V

M

M

V

R

A

E

E

A

T

F

E

A

N

G

I

V

W

W

Q

A

I

V

S

A

Q

E

E

E

L

H

S

K

I

V

S

S

M

V

R

R

T

T

A

A

Y

F

V

I

H

V

G

A

L

C

D

K

R

R

L

I

G

-

Q

-

A

-

I

L

N

M

A

A

K

R

G

E

T

M

R

R

A

G

F

L

Y

Y

active site: K112 (= K104), D152 (= D144)
binding 2-oxoglutaric acid: K76 (= K68), G78 (= G70), M97 (= M89), K100 (= K92), K112 (= K104), A150 (= A142), R192 (= R184), S355 (= S356)
binding nicotinamide-adenine-dinucleotide: R80 (= R72), D152 (= D144), V153 (≠ M145), T196 (= T188), G224 (= G216), G226 (= G218), N227 (= N219), V228 (≠ A220), D248 (= D240), H249 (≠ S241), A299 (= A300), A300 (= A301), A322 (= A323), N323 (= N324), N348 (= N349)

3aogA Crystal structure of glutamate dehydrogenase (gdhb) from thermus thermophilus (glu bound form)
45% identity, 97% coverage: 10:425/428 of query aligns to 17:420/421 of 3aogA

query
sites
3aogA

S

T

R

E

R

Q

L

V

E

D

R

R

A

V

L

V

Q

P

Y

Y

V

L

S

G

L

R

S

L

E

A

D

P

T

L

K

A

E

E

R

S

L

L

K

K

Y

R

P

P

K

K

A

R

T

V

L

L

I

I

V

V

S

D

I

V

P

P

V

V

R

R

M

L

D

D

G

G

S

S

L

V

R

A

V

Y

F

F

Q

E

G

G

Y

Y

R

R

V

V

R

H

Y

H

D

N

D

T

T
x
A

R

R

G

G

P

P

T

A

K

K

G

G

G
|
G

I

V

R

R

Y

Y

H

H

P

P

N

E

V

V

S

T

I

L

D

S

E

E

V

V

K

M

S

A

L

L

A

A

F

G

W

W

M
|
M

T

T

F

I

K

K

C

N

A

A

V

A

V

V

S

G

L

L

P

P

F

Y

G

G

A

G

K
|
K

G

G

I

I

T

R

V

V

N

D

P

P

K

R

E

K

L

L

S

S

R

P

M

G

E

E

L

L

E

E

R

R

L

L

S

T

R

R

G

R

Y

Y

I

T

D

S

A

E

I

I

A

G

D

I

F

L

I

L

G

G

P

P

D

D

T

R

D

D

I

I

P

P

A

A

P
|
P

D
|
D

M

V

Y

N

T

T

N

G

P

E

M

R

I

E

M

M

G

A

W

W

M

M

D
|
D

E

T

Y

Y

S

S

I
x
M

I

N

R

V

R

G

Q

R

L

T

S

V

P

P

A

G

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

A

I

L

G

G

S

S

L

L

G

G

R

R

N

R

T

D

A

A

T

T

A

G

M

R

G

G

A

V

F

F

F

I

A

T

I

A

E

A

T

A

I

A

M

A

P

E

K

K

F

I

E

G

Y

L

T

Q

P

V

E

E

K

G

T

A

T

R

V

V

A

A

I

I

Q

Q

G

G

F

F

G

G

N

N

A

V

G

G

A

N

F

A

L

A

A

A

E

R

L

A

L

F

C

H

L

D

H

H

G

G

Y

A

K

R

V

V

A

A

V

V

S

Q

D

D

S

H

Q

T

G

G

G

T

I

V

Y

Y

S

N

A

E

Q

A

G

G

L

I

D

D

-

P

I

Y

P

D

S

L

I

L

R

R

Q

H

Y

V

K

Q

E

E

V

F

N

G

K

-

Q

-

I

-

Q

-

A

-

V

-

Y

-

C

-

K

-

D

-

S

G

V

V

C

R

N

G

I

Y

V

P

E

K

H

A

T

E

V

P

L

L

T

P

N

A

E

A

E

D

L

F

L

W

A

G

L

L

D

P

V

V

D

E

I

F

L

L

I

V

P

P

A

A

L

L

E

E

N

K

Q

Q

I

I

T

T

E

E

E

Q

N

N

V

A

K

W

D

R

I

I

Q

R

A

A

K

R

F

I

I

V

F

A

E

E

A

G

A

A

N

N

G

G

P

P

T

T

S

P

G

A

A

A

D

D

K

D

I

I

L

L

E

L

D

E

R

K

G

G

V

V

Y

L

V

V

F

V

P

P

D

D

I

V

L

I

I

A

N

N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

D

R

F

S

N

G

S

L

Y

Y

F

W

W

T

T

L

E

D

E

E

E

V

I

N

N

Q

A

K

R

L

L

K

E

A

R

K

V

M

L

V

R

A

N

E

A

T

F

E

E

N

A

I

V

W

W

Q

Q

I

V

S

A

Q

Q

E

E

L

K

S

K

I

I

S

P

M

L

R

R

T

T

A

A

Y

Y

V

V

H

V

G

A

L

A

D

T

R

R

L

V

G

-

Q

-

A

-

I

L

N

E

A

A

K

R

G

A

T

L

R
|
R

A
x
G

F
x
L

Y
|
Y

active site: K111 (= K104), D151 (= D144)
binding glutamic acid: A70 (≠ T63), G77 (= G70), M96 (= M89), K111 (= K104), P150 (= P143), D151 (= D144), D164 (= D157), M168 (≠ I161), S354 (= S356), R417 (= R422), G418 (≠ A423), L419 (≠ F424), Y420 (= Y425)

3aoeB Crystal structure of hetero-hexameric glutamate dehydrogenase from thermus thermophilus (leu bound form)
45% identity, 97% coverage: 10:425/428 of query aligns to 20:423/424 of 3aoeB

query
sites
3aoeB

S

T

R

E

R

Q

L

V

E

D

R

R

A

V

L

V

Q

P

Y

Y

V

L

S

G

L

R

S

L

E

A

D

P

T

L

K

A

E

E

R

S

L

L

K

K

Y

R

P

P

K

K

A

R

T

V

L

L

I

I

V

V

S

D

I

V

P

P

V

V

R

R

M

L

D

D

G

G

S

S

L

V

R

A

V

Y

F

F

Q

E

G

G

Y

Y

R

R

V

V

R

H

Y

H

D

N

D

T

T
x
A

R

R

G

G

P

P

T

A

K

K

G

G

I

V

R

R

Y

Y

H

H

P

P

N

E

V

V

S

T

I

L

D

S

E

E

V

V

K

M

S

A

L

L

A

A

F

G

W

W

M

M

T

T

F

I

K

K

C

N

A

A

V

A

V

V

S

G

L

L

P

P

F

Y

G

G

A

G

K
|
K

G

G

I

I

T

R

V

V

N

D

P

P

K

R

E

K

L

L

S

S

R

P

M

G

E

E

L

L

E

E

R

R

L

L

S

T

R

R

G

R

Y

Y

I

T

D

S

A

E

I

I

A

G

D

I

F

L

I

L

G

G

P

P

D

D

T

R

D

D

I

I

P

P

A

A

P

P

D
|
D

M

V

Y

N

T

T

N

G

P

E

M

R

I

E

M

M

G

A

W

W

M

M

D

D

E

T

Y

Y

S

S

I

M

I

N

R

V

R

G

Q

R

L

T

S

V

P

P

A

G

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

A

I

L

G

G

S

S

L

L

G

G

R

R

N

R

T

D

A

A

T

T

A

G

M

R

G

G

A

V

F

F

F

I

A

T

I

A

E

A

T

A

I

A

M

A

P

E

K

K

F

I

E

G

Y

L

T

Q

P

V

E

E

K

G

T

A

T

R

V

V

A

A

I

I

Q

Q

G

G

F

F

G

G

N

N

A

V

G

G

A

N

F

A

L

A

A

A

E

R

L

A

L

F

C

H

L

D

H

H

G

G

Y

A

K

R

V

V

A

A

V

V

S

Q

D

D

S

H

Q

T

G

G

G

T

I

V

Y

Y

S

N

A

E

Q

A

G

G

L

I

D

D

-

P

I

Y

P

D

S

L

I

L

R

R

Q

H

Y

V

K

Q

E

E

V

F

N

G

K

-

Q

-

I

-

Q

-

A

-

V

-

Y

-

C

-

K

-

D

-

S

G

V

V

C

R

N

G

I

Y

V

P

E

K

H

A

T

E

V

P

L

L

T

P

N

A

E

A

E

D

L

F

L

W

A

G

L

L

D

P

V

V

D

E

I

F

L

L

I

V

P

P

A

A

L

L

E

E

N

K

Q

Q

I

I

T

T

E

E

E

Q

N

N

V

A

K

W

D

R

I

I

Q

R

A

A

K

R

F

I

I

V

F

A

E

E

A

G

A

A

N

N

G

G

P

P

T

T

S

P

G

A

A

A

D

D

K

D

I

I

L

L

E

L

D

E

R

K

G

G

V

V

Y

L

V

V

F

V

P

P

D

D

I

V

L

I

I

A

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

D

R

F

S

N

G

S

L

Y

Y

F

W

W

T

T

L

E

D

E

E

E

V

I

N

N

Q

A

K

R

L

L

K

E

A

R

K

V

M

L

V

R

A

N

E

A

T

F

E

E

N

A

I

V

W

W

Q

Q

I

V

S

A

Q

Q

E

E

L

K

S

K

I

I

S

P

M

L

R

R

T

T

A

A

Y

Y

V

V

H

V

G

A

L

A

D

T

R

R

L

V

G

-

Q

-

A

-

I

L

N

E

A

A

K
x
R

G

A

T

L

R
|
R

A
x
G

F

L

Y
|
Y

active site: K114 (= K104), D154 (= D144)
binding leucine: A73 (≠ T63), R417 (≠ K419), R420 (= R422), G421 (≠ A423), Y423 (= Y425)

8owmC Crystal structure of glutamate dehydrogenase 2 from arabidopsis thaliana binding ca, NAD and 2,2-dihydroxyglutarate (see paper)
43% identity, 97% coverage: 10:423/428 of query aligns to 10:413/413 of 8owmC

query
sites
8owmC

S

N

R

R

R

N

L

F

E

R

R

H

A

A

L

S

Q

R

Y

I

V

L

S

G

L

L

S

D

E

S

D

K

T

I

K

E

E

R

R
x
S

L

L

K

M

Y
x
I

P

P

K

F

A

R

T

E

L

I

I

K

V

V

S

E

I

C

P

T

V

I

R

P

M

K

D

D

G

G

S

T

L

L

R

V

V

S

F

Y

Q

I

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

D

D

N

T

A

R

R

G

G

P

P

T

M

K
|
K

G

G

G
|
G

I

I

R

R

Y

Y

H

H

P

P

N

E

V

V

S

D

I

P

D

D

E

E

V

V

K

N

S

A

L

L

A

A

F

Q

W

L

M
|
M

T

T

F

W

K
|
K

C

T

A

A

V

V

V

A

S

D

L

I

P

P

F

Y

G

G

A

A

K
|
K

G

G

I

I

T

G

V

C

N

S

P

P

K

R

E

D

L

L

S

S

R

L

M

S

E

E

L

L

E

E

R

R

L

L

S

T

R

R

G

V

Y

F

I

T

D

Q

A

K

I

I

A

H

D

D

F

L

I

I

G

G

P

I

D

H

T

T

D

D

I

V

P

P

A
|
A

P

P

D
|
D

M
|
M

Y

G

T

T

N

N

P

A

M

Q

I

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

Y

Y

S

S

I

K

I

F

R

H

R

G

Q

H

L

-

S

S

P

P

A

A

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

D

I

L

G

G

S

S

L

L

G

G

R
|
R

N

E

T

A

A

A

T
|
T

A

G

M

R

G

G

A

V

F

V

F

F

A

A

I

T

E

E

T

A

I

L

M

L

P

A

K

E

F

Y

E

G

Y

K

T

S

P

I

E

Q

K

G

T

L

T

T

V

F

A

V

I

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

A
x
V

G

G

A

T

F

W

L

A

A

A

E

K

L

L

L

I

C

H

L

E

H

K

G

G

Y

G

K

K

V

V

A

A

V

V

S

S

D
|
D

S
x
I

Q

T

G

G

G

A

I

I

Y

R

S

N

A

P

Q

E

G

G

L

I

D

D

I

I

P

N

S

A

I

L

R

I

Q

K

Y

H

K

K

E

D

V

A

N

T

K

G

Q

S

I

L

Q

N

A

D

V

F

Y

N

C

G

K

G

D

D

S

A

V

-

C

-

N

-

I

-

V

-

E

-

H

-

T

-

V

-

L

M

T

N

N

S

E

D

E

E

L

L

A

I

L

H

D

E

V

C

D

D

I

V

L

L

I

I

P

P

A
x
C

A
|
A

L

L

E

G

N

G

Q

V

I

L

T

N

E

K

E

E

N

N

V

A

K

G

D

D

I

V

Q

K

A

A

K

K

F

F

I

I

F

V

E

E

A

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

S

P

G

D

A

A

D

D

K

E

I

I

L

L

E

S

D

K

R

K

G

G

V

V

Y

I

V

I

F

L

P

P

D

D

I

I

L

Y

I

A

N
|
N

A

A

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

Q

G

G

L

F

Y

M

W

W

T

E

L

E

D

E

E

K

V

V

N

N

Q

L

K

E

L

L

K

Q

A

K

K

Y

M

M

V

T

A

R

E

A

T

F

E

H

N

N

I

I

W

K

Q

T

I

M

S

C

Q

H

E

T

L

H

S

S

I

C

S

N

M

L

R

R

T

M

A

G

A

A

Y

F

V

T

H

L

G

G

L

V

D

N

R

R

L

V

G

A

Q

R

A

A

I

T

N

Q

A

L

K

R

G

G

T

W

R

E

A

A

binding calcium ion: S29 (≠ R29), I32 (≠ Y32)
binding nicotinamide-adenine-dinucleotide: D144 (= D144), M145 (= M145), R183 (= R184), T187 (= T188), F216 (= F217), G217 (= G218), N218 (= N219), V219 (≠ A220), D239 (= D240), I240 (≠ S241), C290 (≠ A300), A291 (= A301), A313 (= A323), N314 (= N324), N339 (= N349)
binding 2,2-bis(oxidanyl)pentanedioic acid: K68 (= K68), G70 (= G70), M89 (= M89), K92 (= K92), K104 (= K104), A142 (= A142), R183 (= R184), N314 (= N324), V343 (= V353), S346 (= S356)

6yehA Arabidopsis thaliana glutamate dehydrogenase isoform 1 in apo form (see paper)
43% identity, 96% coverage: 10:420/428 of query aligns to 7:407/410 of 6yehA

query
sites
6yehA

S

N

R

R

R

N

L

F

E

K

R

L

A

A

L

A

Q

R

Y

L

V

L

S

G

L

L

S

D

E

S

D

K

T

L

K

E

E

K

R
x
S

L
|
L

K

L

Y
x
I

P
|
P

K

F

A

R

T

E

L

I

I

K

V

V

S

E

I

C

P

T

V

I

R

P

M

K

D

D

G

G

S

T

L

L

R

A

V

S

F

F

Q

V

G

G

Y

F

R

R

V

V

R

Q

Y

H

D
|
D

D

N

T

A

R

R

G

G

P

P

T

M

K

K

G

G

I

I

R

R

Y

Y

H

H

P

P

N

E

V

V

S

D

I

P

D

D

E

E

V

V

K

N

S

A

L

L

A

A

F

Q

W

L

M

M

T

T

F

W

K

K

C

T

A

A

V

V

V

A

S

K

L

I

P

P

F

Y

G

G

A

A

K

K

G

G

I

I

T

G

V

C

N

D

P

P

K

S

E

K

L

L

S

S

R

I

M

S

E

E

L

L

E

E

R

R

L

L

S

T

R

R

G

V

Y

F

I

T

D

Q

A

K

I

I

A

H

D

D

F

L

I

I

G

G

P

I

D

H

T

T

D

D

I

V

P

P

A

A

P

P

D

D

M

M

Y

G

T

T

N

G

P

P

M

Q

I

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

Y

Y

S

S

I

K

I

F

R

-

R

H

Q

G

L

Y

S

S

P

P

A

A

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

D

I

L

G

G

S

S

L

L

G

G

R

R

N

D

T

A

A

A

T

T

A

G

M

R

G

G

A

V

F

M

F

F

A

G

I

T

E

E

T

A

I

L

M

L

P

N

K

E

F

H

E

G

Y

K

T

T

P

I

E

S

K

G

T

Q

T

R

V

F

A

V

I

I

Q

Q

G

G

F

F

G

G

N

N

A

V

G

G

A

S

F

W

L

A

A

A

E

K

L

L

L

I

C

S

L

E

H

K

G

G

Y

G

K

K

V

I

V

V

A

A

V

V

S

S

D

D

S

I

Q

T

G

G

G

A

I

I

Y

K

S

N

A

K

Q

D

G

G

L

I

D

D

I

I

P

P

S

A

I

L

R

L

Q

K

Y

H

K

T

E

K

V

E

N

H

K

R

Q

G

I

V

Q

K

A

G

V

F

Y

D

C

G

K

A

D

D

S

P

V

I

C

-

N

-

I

-

V

-

E

-

H

-

T

-

V

-

L

-

T

D

N

P

E

N

E

S

L

I

L

L

A

V

L

E

D

D

V

C

D

D

I

I

L

L

I

V

P

P

A

A

L

L

E

G

N

G

Q

V

I

I

T

N

E

R

E

E

N

N

V

A

K

N

D

E

I

I

Q

K

A

A

K

K

F

F

I

I

F

I

E

E

A

A

N

N

G

H

P

P

T

T

T

D

S

P

G

D

A

A

D

D

K

E

I

I

L

L

E

S

D

K

R

K

G

G

V

V

Y

V

V

I

F

L

P

P

D

D

I

I

L

Y

I

A

N

N

A

S

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

Q

G

G

L

F

Y

M

W

W

T

E

L

E

D

E

E

K

V

V

N

N

Q

D

K

E

L

L

K

K

A

T

K

Y

M

M

V

T

A

R

E

S

T

F

E

K

N

D

I

L

W

K

Q

E

I

M

S

C

Q

K

E

T

L

H

S

S

I

C

S

D

M

L

R

R

T

M

A

G

A

A

Y

F

V

T

H

L

G

G

L

V

D

N

R

R

L

V

G

A

Q

Q

A

A

I

T

N

I

A

L

K

R

G

G

binding potassium ion: S26 (≠ R29), L27 (= L30), I29 (≠ Y32), P30 (= P33), D58 (= D61)

6yeiF Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD (see paper)
43% identity, 96% coverage: 10:420/428 of query aligns to 8:408/410 of 6yeiF

query
sites
6yeiF

S

N

R

R

R

N

L

F

E

K

R

L

A

A

L

A

Q

R

Y

L

V

L

S

G

L

L

S

D

E

S

D

K

T

L

K

E

E

K

R
x
S

L
|
L

K

L

Y
x
I

P
|
P

K
x
F

A

R

T

E

L

I

I

K

V

V

S

E

I

C

P

T

V

I

R

P

M

K

D

D

G

G

S

T

L

L

R

A

V

S

F

F

Q

V

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

D

D

N

T

A

R

R

G

G

P

P

T

M

K
|
K

G

G

G
|
G

I

I

R
|
R

Y

Y

H

H

P

P

N

E

V

V

S

D

I

P

D

D

E

E

V

V

K

N

S

A

L

L

A

A

F

Q

W

L

M
|
M

T

T

F

W

K
|
K

C

T

A

A

V

V

V

A

S

K

L

I

P

P

F

Y

G

G

A

A

K
|
K

G

G

I

I

T

G

V

C

N

D

P

P

K

S

E

K

L

L

S

S

R

I

M

S

E

E

L

L

E

E

R

R

L

L

S

T

R

R

G

V

Y

F

I

T

D

Q

A

K

I

I

A

H

D

D

F

L

I

I

G

G

P

I

D

H

T

T

D

D

I

V

P

P

A
|
A

P

P

D
|
D

M
|
M

Y

G

T

T

N

G

P

P

M

Q

I

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

Y

Y

S

S

I

K

I

F

R

-

R

H

Q

G

L

Y

S

S

P

P

A

A

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

D

I

L

G

G

S

S

L

L

G

G

R

R

N

D

T

A

A

A

T
|
T

A

G

M

R

G

G

A

V

F

M

F

F

A

G

I

T

E

E

T

A

I

L

M

L

P

N

K

E

F

H

E

G

Y

K

T

T

P

I

E

S

K

G

T

Q

T

R

V

F

A

V

I

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

A
x
V

G

G

A

S

F

W

L

A

A

A

E

K

L

L

L

I

C

S

L

E

H

K

G

G

Y

G

K

K

V

I

V

V

A

A

V

V

S

S

D
|
D

S
x
I

Q

T

G

G

G

A

I

I

Y

K

S

N

A

K

Q

D

G

G

L

I

D

D

I

I

P

P

S

A

I

L

R

L

Q

K

Y

H

K

T

E

K

V

E

N

H

K

R

Q

G

I

V

Q

K

A

G

V

F

Y

D

C

G

K

A

D

D

S

P

V

I

C

-

N

-

I

-

V

-

E

-

H

-

T

-

V

-

L

-

T

D

N

P

E

N

E

S

L

I

L

L

A

V

L

E

D

D

V

C

D

D

I

I

L

L

I

V

P

P

A
|
A

L

L

E

G

N

G

Q

V

I

I

T

N

E

R

E

E

N

N

V

A

K

N

D

E

I

I

Q

K

A

A

K

K

F

F

I

I

F

I

E

E

A

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

S

P

G

D

A

A

D

D

K

E

I

I

L

L

E

S

D

K

R

K

G

G

V

V

Y

V

V

I

F

L

P

P

D

D

I

I

L

Y

I

A

N
|
N

A

S

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

Q

G

G

L

F

Y

M

W

W

T

E

L

E

D

E

E

K

V

V

N

N

Q

D

K

E

L

L

K

K

A

T

K

Y

M

M

V

T

A

R

E

S

T

F

E

K

N

D

I

L

W

K

Q

E

I

M

S

C

Q

K

E

T

L

H

S

S

I

C

S

D

M

L

R

R

T

M

A

G

A

A

Y

F

V

T

H

L

G

G

L

V

D

N

R

R

L

V

G

A

Q

Q

A

A

I

T

N

I

A

L

K

R

G

G

binding 2-oxoglutaric acid: K66 (= K68), G68 (= G70), M87 (= M89), K90 (= K92), K102 (= K104), A140 (= A142), V341 (= V353), S344 (= S356)
binding potassium ion: S27 (≠ R29), L28 (= L30), I30 (≠ Y32), P31 (= P33), F32 (≠ K34)
binding nicotinamide-adenine-dinucleotide: R70 (= R72), D142 (= D144), M143 (= M145), T185 (= T188), F214 (= F217), G215 (= G218), N216 (= N219), V217 (≠ A220), D237 (= D240), I238 (≠ S241), A288 (= A300), A289 (= A301), A311 (= A323), N312 (= N324), N337 (= N349)

6yeiA Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD (see paper)
43% identity, 96% coverage: 10:420/428 of query aligns to 7:407/409 of 6yeiA

query
sites
6yeiA

S

N

R

R

R

N

L

F

E

K

R

L

A

A

L

A

Q

R

Y

L

V

L

S

G

L

L

S

D

E

S

D

K

T

L

K

E

E

K

R
x
S

L
|
L

K

L

Y
x
I

P
|
P

K

F

A

R

T

E

L

I

I

K

V

V

S

E

I

C

P

T

V

I

R

P

M

K

D

D

G

G

S

T

L

L

R

A

V

S

F

F

Q

V

G

G

Y

F

R

R

V

V

R

Q

Y

H

D

D

D

N

T

A

R

R

G

G

P

P

T

M

K

K

G

G

I

I

R

R

Y

Y

H

H

P

P

N

E

V

V

S

D

I

P

D

D

E

E

V

V

K

N

S

A

L

L

A

A

F

Q

W

L

M

M

T

T

F

W

K

K

C

T

A

A

V

V

V

A

S

K

L

I

P

P

F

Y

G

G

A

A

K

K

G

G

I

I

T

G

V

C

N

D

P

P

K

S

E

K

L

L

S

S

R

I

M

S

E

E

L

L

E

E

R

R

L

L

S

T

R

R

G

V

Y

F

I

T

D

Q

A

K

I

I

A

H

D

D

F

L

I

I

G

G

P

I

D

H

T

T

D

D

I

V

P

P

A

A

P

P

D

D

M

M

Y

G

T

T

N

G

P

P

M

Q

I

T

M

M

G

A

W

W

M

I

M

L

D

D

E

E

Y

Y

S

S

I

K

I

F

R

-

R

H

Q

G

L

Y

S

S

P

P

A

A

V

V

I

V

T

T

G

G

K

K

P

P

V

I

S

D

I

L

G

G

S

S

L

L

G

G

R

R

N

D

T

A

A

A

T
|
T

A

G

M

R

G

G

A

V

F

M

F

F

A

G

I

T

E

E

T

A

I

L

M

L

P

N

K

E

F

H

E

G

Y

K

T

T

P

I

E

S

K

G

T

Q

T

R

V

F

A

V

I

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

A
x
V

G

G

A

S

F

W

L

A

A

A

E

K

L

L

L

I

C

S

L

E

H

K

G

G

Y

G

K

K

V

I

V

V

A

A

V

V

S

S

D
|
D

S
x
I

Q

T

G

G

G

A

I

I

Y

K

S

N

A

K

Q

D

G

G

L

I

D

D

I

I

P

P

S

A

I

L

R

L

Q

K

Y

H

K

T

E

K

V

E

N

H

K

R

Q

G

I

V

Q

K

A

G

V

F

Y

D

C

G

K

A

D

D

S

P

V

I

C

-

N

-

I

-

V

-

E

-

H

-

T

-

V

-

L

-

T

D

N

P

E

N

E

S

L

I

L

L

A

V

L

E

D

D

V

C

D

D

I

I

L

L

I

V

P

P

A

A

A
|
A

L
|
L

E

G

N

G

Q

V

I

I

T

N

E

R

E

E

N

N

V

A

K

N

D

E

I

I

Q

K

A

A

K

K

F

F

I

I

F

I

E

E

A

A

A
|
A

N
|
N

G

H

P

P

T

T

T

D

S

P

G

D

A

A

D

D

K

E

I

I

L

L

E

S

D

K

R

K

G

G

V

V

Y

V

V

I

F

L

P

P

D

D

I

I

L

Y

I

A

N
|
N

A

S

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

S

Q

G

G

L

F

Y

M

W

W

T

E

L

E

D

E

E

K

V

V

N

N

Q

D

K

E

L

L

K

K

A

T

K

Y

M

M

V

T

A

R

E

S

T

F

E

K

N

D

I

L

W

K

Q

E

I

M

S

C

Q

K

E

T

L

H

S

S

I

C

S

D

M

L

R

R

T

M

A

G

A

A

Y

F

V

T

H

L

G

G

L

V

D

N

R

R

L

V

G

A

Q

Q

A

A

I

T

N

I

A

L

K

R

G

G

binding potassium ion: S26 (≠ R29), L27 (= L30), I29 (≠ Y32), P30 (= P33)
binding nicotinamide-adenine-dinucleotide: T184 (= T188), F213 (= F217), G214 (= G218), N215 (= N219), V216 (≠ A220), D236 (= D240), I237 (≠ S241), A288 (= A301), L289 (= L302), A310 (= A323), N311 (= N324), N336 (= N349)

P28997 NAD-specific glutamate dehydrogenase; NAD-GDH; EC 1.4.1.2 from Peptoniphilus asaccharolyticus (Peptostreptococcus asaccharolyticus) (see 2 papers)
41% identity, 98% coverage: 1:420/428 of query aligns to 1:419/421 of P28997

query
sites
P28997

M

M

S

T

D

D

S

T

L

L

-

N

-

P

F

L

D

V

D

A

A

A

S

Q

R

E

R

K

L

V

E

R

R

I

A

A

L

C

Q

E

Y

K

V

L

S

G

L

C

S

D

E

P

D

A

T

V

K

Y

E

E

R

L

L

L

K

K

Y

E

P

P

K

Q

A

R

T

V

L

I

I

E

V

I

S

S

I

I

P

P

V

V

R

K

M

M

D

D

G

G

S

T

L

V

R

K

V

V

F

F

Q

K

G

G

Y

W

R

R

V

S

R

A

Y

H

D

S

T

A

R

V

G

G

P

P

T

S

K

K

G

G

I

V

R

R

Y

F

H

H

P

P

N

N

V

V

S

N

I

M

D

D

E

E

V

V

K

K

S

A

L

L

A

S

F

L

W

W

M

M

T

T

F

F

K

K

C

G

A

G

V

A

V

L

S

G

L

L

P

P

F

Y

G

G

A

G

K

K

G

G

I

I

T

C

V

V

N

D

P

P

K

A

E

E

L

L

S

S

R

E

M

R

E

E

L

L

E

E

R

Q

L

L

S

S

R

R

G

G

Y

W

I

V

D

R

A

G

I

L

A

Y

D

K

F

Y

I

L

G

G

P

D

D

R

T

I

D

D

I

I

P

P

A

A

P

P

D

D

M

V

Y

N

T

T

N

N

P

G

M

Q

I

I

M

M

G

S

W

W

M

F

M

V

D

D

E

E

Y

Y

S

V

I

K

I

L

R

N

-

G

R

E

Q

R

L

M

S

D

P

I

A

G

V

T

I

F

T

T

G

G

K

K

P

P

V

V

S

A

I

F

G

G

S

S

L

E

G

G

R

R

N

N

T

E

A

A

T

T

A

G

M

F

G

G

A

V

F

A

F

V

A

V

I

V

E

R

T

E

I

S

M

A

P

K

K

R

F

F

E

G

Y

I

T

K

P

M

E

E

K

D

T

A

T

K

V

I

A

A

I

V

Q

Q

G

G

F

F

G

G

N

N

A

V

G

G

A

T

F

F

L

T

A

V

E

K

L

N

L

I

C

E

L

R

H

Q

G

G

Y

G

K

K

V

V

V

C

A

A

V

I

S

A

D
x
E

-
x
W

-
x
D

-

R

S

N

Q

E

G

G

-

N

-

Y

G

A

I

L

Y

Y

S

N

A

E

Q

N

G

G

L

I

D

D

I

F

P

K

S

E

I

L

R

L

Q

A

Y

Y

K

K

E

E

V

A

N

N

K

K

Q

T

I

L

Q

I

A

G

V

F

Y

P

C

G

K

A

D

E

S

R

V

I

C

-

N

-

I

-

V

-

E

-

H

-

T

-

V

-

L

-

T

T

N

D

E

E

L

F

L

W

A

T

L

K

D

E

V

Y

D

D

I

I

L

I

I

V

P

P

A

A

L

L

E

E

N

N

Q

V

I

I

T

T

E

G

E

E

N

R

V

A

K

K

D

T

I

I

Q

N

A

A

K

K

F

L

I

V

F

C

E

E

A

A

N

N

G

G

P

P

T

T

S

P

G

E

A

G

D

D

K

K

I

V

L

L

E

T

D

E

R

R

G

G

V

I

Y

N

V

L

F

T

P

P

D

D

I

I

L

L

I

T

N

N

A

S

G

G

V

V

T

L

V

V

S

S

Y

Y

F

Y

E

E

W

W

V

V

Q

Q

N

N

R

Q

S

Y

G

G

L

Y

Y

Y

W

W

T

T

L

E

D

A

E

E

V

V

N

E

Q

E

K

K

L

Q

K

E

A

A

K

D

M

M

V

M

A

K

E

A

T

I

E

K

N

G

I

V

W

F

Q

A

I

V

S

A

Q

D

E

E

L

Y

S

N

I

V

S

T

M

L

R

R

T

E

A

A

A

V

Y

Y

V

M

H

Y

G

A

L

I

D

K

R

S

L

I

G

D

Q

V

A

A

I

M

N

K

A

L

K

R

G

G

E243 (≠ D240) Important for nucleotide recognition; mutation to D: Shows a 9-fold relaxation of the strong discrimination against NADPH due to the decrease of binding affinity for NADH and the increase for NADPH.; mutation to K: Severely crippled in its ability to bind to NADH. Decrease of binding affinity for NADH and increase for NADPH.; mutation to R: Decrease of binding affinity for NADH and increase for NADPH.
W244 (vs. gap) mutation to S: Decrease of binding affinity for NADH and increase for NADPH.
D245 (vs. gap) mutation to K: Decrease of binding affinity for NADH and increase for NADPH.

P80053 Glutamate dehydrogenase 2; GDH-2; EC 1.4.1.3 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
41% identity, 98% coverage: 1:420/428 of query aligns to 6:418/420 of P80053

query
sites
P80053

M

L

S

S

D

S

S

S

L

L

F

Y

D

T

D

Q

A

Q

S

V

R

K

L

L

E

Y

R

K

A

V

L

G

Q

E

Y

L

V

L

S

G

L

L

S

D

E

N

D

E

T

T

K

L

E

E

R

T

L

L

K

S

Y

Q

P

P

K

E

A

R

T

I

L

I

I

Q

V

V

S

K

I

I

P

Q

V

I

R

R

M

G

D

S

D

D

G

G

S

K

L

L

R

K

V

T

F

F

Q

M

G

G

Y

W

R

R

V

S

R

Q

Y

H

D

N

D

S

T

A

R

L

G

G

P

P

T

Y

K

K

G

G

I

V

R

R

Y

Y

H

H

P

P

N

N

V

V

S

T

I

Q

D

D

E

E

V

V

K

E

S

A

L

L

A

S

F

M

W

I

M

M

T

T

F

W

K

K

C

N

A

S

V

L

S

L

L

L

P

P

F

Y

G

G

A

G

K

K

G

G

I

V

T

R

V

V

N

D

P

P

K

K

E

K

L

L

S

T

R

R

M

E

E

E

L

L

E

E

R

Q

L

L

S

S

R

R

G

K

Y

Y

I

I

D

Q

A

A

I

I

A

Y

D

K

F

Y

I

L

G

G

P

S

D

E

T

L

D

D

I

I

P

P

A

A

P

P

D

D

M

V

Y

N

T

T

N

D

P

S

M

Q

I

T

M

M

G

A

W

W

M

F

M

L

D

D

E

E

Y

Y

S

I

I

K

I

I

R

T

R

G

Q

K

L

V

S

D

P

F

A

A

V

V

I

F

T

T

G

G

K

K

P

P

V

V

S

E

I

L

G

G

S

I

L

G

G

V

R

R

N

L

T

Y

A

S

T

T

A

G

M

L

G

G

A

V

F

A

F

T

A

I

I

A

E

K

T

E

I

A

M

A

P

N

K

K

F

F

E

I

Y

G

T

G

P

V

E

E

K

E

T

A

T

R

V

V

A

I

I

I

Q

Q

G

G

F

F

G

G

N

N

A

V

G

G

A

Y

F

Y

L

A

A

G

E

K

L

F

L

L

C

S

L

E

H

M

G

G

Y

A

K

K

V

I

V

V

A

G

V

V

S

S

D

D

S

S

Q

K

G

G

I

V

Y

I

S

N

A

E

Q
x
K

G

G

L

I

D

D

I

-

P

-

S

-

I

-

R

-

Q

-

Y

-

K

-

E

-

V

V

N

G

K
|
K

Q

A

I

I

Q

E

A

I

V

K

Y

E

C

K

K

T

D

G

S

S

V

V

C

I

N

N

I

Y

V

P

E

E

H

G

T

R

V

K

L

V

T

T

N

N

E

E

L

L

A

I

L

S

D

D

V

C

D

D

I

I

L

L

I

I

P

P

A

A

L

L

E

E

N

N

Q

V

I

I

T

N

E

K

E

F

N

N

V

A

K

P

D

K

I

V

Q

K

A

A

K

K

F

L

I

I

F

V

E

E

A

G

A

A

N

N

G

G

P

P

T

L

T

T

S

A

G

D

A

A

D

D

K

E

I

I

L

M

E

R

D

Q

R

R

G

G

V

I

Y

A

V

V

F

V

P

P

D

D

I

I

L

L

I

A

N

N

A

A

G

G

V

V

T

V

V

G

S

S

Y

Y

F

V

E

E

W

W

V

A

Q

N

N

N

R

K

S

M

G

G

L

E

Y

I

W

I

T

S

L

D

D

E

E

E

V

A

N

K

Q
x
K

K

L

L

I

K

V

A

D

K

R

M

M

V

N

A

N

E

A

T

F

E

N

N

T

I

L

W

Y

Q

D

I

Y

S

H

Q

Q

E
x
K

L
x
K

-

L

-

E

S

D

I

H

S

D

M

L

R

R

T

T

A

A

Y

M

V

A

H

L

G

A

L

V

D

D

R

R

L

V

G

V

Q

R

A

A

I

M

N

K

A

A

K

R

G

G

K254 (≠ Q249) modified: N6-methyllysine
K260 (= K264) modified: N6-methyllysine
K372 (≠ Q376) modified: N6-methyllysine
K391 (≠ E395) modified: N6-methyllysine
K392 (≠ L396) modified: N6-methyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylmethionine

1v9lA L-glutamate dehydrogenase from pyrobaculum islandicum complexed with NAD (see paper)
43% identity, 95% coverage: 13:420/428 of query aligns to 4:416/418 of 1v9lA

query
sites
1v9lA

L

L

E

E

R

Y

A

V

L

L

Q

N

Y

Y

V

V

-

K

-

G

-

V

-

E

-

L

-

G

S

G

L

F

S

P

E

E

D

D

T

F

K

Y

E

K

R

I

L

L

K

S

Y

R

P

P

K

R

A

R

T

V

L

L

I

I

V

V

S

N

I

I

P

P

V

V

R

R

M

L

D

D

D

G

G

G

S

G

L

F

R

E

V

V

F

F

Q

E

G

G

Y

Y

R

R

V

V

R

Q

Y

H

D

C

D

D

T

V

R

L

G

G

P

P

T

Y

K

K

G

G

I

V

R

R

Y

F

H

H

P

P

N

E

V

V

S

T

I

L

D

A

E

D

V

D

K

V

S

A

L

L

A

A

F

I

W

L

M

M

T

T

F

L

K

K

C

N

A

S

V

L

V

A

S

G

L

L

P

P

F

Y

G

G

A

A

K
|
K

G

G

G

A

I

V

T

R

V

V

N

D

P

P

K

K

E

K

L

L

S

S

R

Q

M

R

E

E

L

L

E

E

R

E

L

L

S

S

R

R

G

G

Y

Y

I

A

D

R

A

A

I

I

A

A

D

P

F

L

I

I

G

G

P

D

D

V

T

V

D

D

I

I

P

P

A

A

P

P

D
|
D

M

V

Y

G

T

T

N

N

P

A

M

Q

I

I

M

M

G

A

W

W

M

M

M

V

D

D

E

E

Y

Y

S

S

I

K

I

I

R

K

R

G

Q

Y

L

N

S

V

P

P

A

G

V

V

I

F

T

T

G

S

K

K

P

P

V

P

S

E

I

L

G

W

G

G

S

N

L

P

G

V

R

R

N

E

T

Y

A

A

T
|
T

A

G

M

F

G

G

A

V

F

A

F

V

A

A

I

T

E

R

T

E

I

M

M

A

P

K

K

K

F

L

E

W

Y

G

T

G

P

I

E

E

K

G

T

K

T

T

V

V

A

A

I

I

Q
|
Q

G

G

F

M

G
|
G

N
|
N

A
x
V

G

G

A

R

F

W

L

T

A

A

E

Y

L

W

L

L

C

E

L

K

H

M

G

G

Y

A

K

K

V

V

V

I

A

A

V

V

S

S

D
|
D

S
x
I

Q

N

G

G

G

V

I

A

Y

Y

S

R

A

K

Q

E

G

G

L

L

D

N

I

V

P

E

S

L

I

I

R

Q

Q

K

Y

N

K

K

E

G

V

L

N

T

K

G

Q

P

-

A

-

L

I

V

Q

E

A

L

V

F

Y

T

C

T

K

K

D

D

S

N

V

A

C

-

N

-

I

-

V

-

E

-

H

E

T

F

V

V

L

K

T

N

N

P

E

D

E

A

L

I

L

F

A

K

L

L

D

D

V

V

D

D

I

I

L

F

I

V

P

P

A

A

A
|
A

L
x
I

E

E

N

N

Q

V

I

I

T

R

E

G

E

D

N

N

V

A

K

G

D

L

I

V

Q

K

A

A

K

R

F

L

I

V

F

V

E

E

A

G

A
|
A

N
|
N

G

G

P

P

T

T

S

P

G

E

A

A

D

E

K

R

I

I

L

L

E

Y

D

E

R

R

G

G

V

V

Y

V

V

V

F

V

P

P

D

D

I

I

L

L

I

A

N
|
N

A

A

G

G

V

V

T

I

V

M

S

S

Y

Y

F

L

E

E

W

W

V

V

Q

E

N

N

R

L

S

Q

G

W

L

Y

Y

I

W

W

T

D

L

E

D

E

E

E

V

T

N

R

Q

K

K

R

L

L

K

E

A

N

K

I

M

M

V

V

A

N

E

N

T

V

E

E

N

R

I

V

W

Y

Q

K

I

R

S

W

Q

Q

-

R

E

E

L

K

S

G

I

W

S

T

M

M

R

R

T

D

A

A

Y

I

V

V

H

T

G

A

L

L

D

E

R

R

L

I

G

Y

Q

N

A

A

I

M

N

K

A

I

K

R

G

G

active site: K102 (= K104), D142 (= D144)
binding nicotinamide-adenine-dinucleotide: T186 (= T188), Q213 (= Q215), G216 (= G218), N217 (= N219), V218 (≠ A220), D238 (= D240), I239 (≠ S241), A296 (= A301), I297 (≠ L302), A318 (= A323), N319 (= N324), N344 (= N349)

8karA Glutamate dehydrogenase-akg
41% identity, 98% coverage: 2:420/428 of query aligns to 1:412/414 of 8karA

query
sites
8karA

S

T

D

S

S

N

L

L

F

L

D

V

D

Q

A

Q

S

V

R

K

L

L

E

Y

R

K

A

V

L

G

Q

E

Y

L

V

L

S

G

L

L

S

D

E

S

D

E

T

T

K

I

E

D

R

V

L

L

K

S

Y

Q

P

P

K

E

A

R

T

V

L

M

I

Q

V

V

S

K

I

I

P

Q

V

I

R

R

M

S

D

S

D

D

G

G

S

K

L

L

R

R

V

T

F

F

Q

I

G

G

Y

W

R

R

V

S

R

Q

Y

H

D

N

D

S

T

A

R

L

G

G

P

P

T

Y

K
|
K

G

G

G
|
G

I

V

R
|
R

Y

Y

H

G

P

P

N

N

V

V

S

T

I

Q

D

D

E

E

V

V

K

I

S

A

L

L

A

S

F

M

W

I

M
|
M

T

T

F

W

K
|
K

C

N

A

S

V

L

S

L

L

L

P

P

F

Y

G

G

A

G

K
|
K

G

G

I

I

T

R

V

V

N

D

P

P

K

K

E

K

L

L

S

T

R

L

M

K

E

E

L

L

E

E

R

D

L

L

S

S

R

R

G

K

Y

Y

I

V

D

Q

A

L

I

I

A

H

D

K

F

Y

I

L

G

G

P

S

D

D

T

V

D

D

I

I

P

P

A
|
A

P

P

D
|
D

M
x
I

Y

N

T

T

N

N

P

P

M

Q

I

T

M

M

G

A

W

W

M

F

M

L

D

D

E

E

Y

Y

S

I

I

K

I

I

R

T

R

G

Q

K

L

V

S

D

P

F

A

A

V

V

I

F

T

T

G

G

K

K

P

P

V

S

S

E

I

L

G

G

S

I

L

G

G

V

R
|
R

N

L

T

Y

A

S

T
|
T

A

G

M

L

G

G

A

V

F

A

F

T

A

I

I

A

E

R

T

D

I

A

M

A

P

N

K

K

F

F

E

I

Y

G

T

G

P

I

E

E

K

G

T

S

T

R

V

V

A

I

I

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

A
x
V

G

G

A

F

F

F

L

T

A

A

E

K

L

F

L

L

C

S

L

E

H

M

G

G

Y

A

K

K

V

I

A

G

V

V

S

S

D
|
D

S
x
I

Q

G

G

G

I

V

Y

I

S

N

A

E

Q

N

G

G

L

I

D

D

I

-

P

-

S

-

I

-

R

-

Q

-

Y

-

K

-

E

-

V

V

N

N

K

R

Q

A

I

L

Q

E

A

V

V

V

Y

Q

C

S

K

T

D

G

S

S

V

V

C

V

N

N

I

Y

V

L

E

E

H

G

T

K

V

K

L

V

T

T

N
|
N

E

E

L

L

A

I

L

S

D

D

V

C

D

D

I

I

L

L

I

I

P

P

A

A

A
|
A

L

V

E

E

N

N

Q

V

I

I

T

N

E

K

E

F

N

N

V

A

K

P

D

K

I

V

Q

R

A

A

K

K

F

L

I

I

F

V

E

E

A

G

A
|
A

N
|
N

G

G

P

P

T

L

T

T

S

A

G

D

A

A

D

D

K

E

I

I

L

M

E

K

D

Q

R

R

G

G

V

V

Y

I

V

V

F

I

P

P

D

D

I

I

L

L

I

A

N
|
N

A

A

G

G

G
|
G

V
|
V

T

V

V

G

S
|
S

Y

Y

F

V

E

E

W

W

V

A

Q

N

N

N

R

R

S

S

G

G

L

G

Y

I

W

I

T

S

L

D

D

E

E

E

V

A

N

K

Q

K

K

L

L

I

K

V

A

D

K

R

M

M

V

I

A

N

E

A

T

F

E

N

N

T

I

L

W

Y

Q

D

I

F

S

H

Q

K

E

R

-

K

-

F

L

A

S

D

I

Q

S

D

M

L

R

R

T

T

A

V

A

A

Y

M

V

A

H

L

G

A

L

V

D

D

R

R

L

V

G

V

Q

G

A

A

I

M

N

K

A

A

K

R

G

G

binding 2-oxoglutaric acid: K67 (= K68), G69 (= G70), M88 (= M89), K91 (= K92), K103 (= K104), A141 (= A142), N314 (= N324), G342 (= G352), V343 (= V353), S346 (= S356)
binding nicotinamide-adenine-dinucleotide: R71 (= R72), D143 (= D144), I144 (≠ M145), R183 (= R184), T187 (= T188), F216 (= F217), G217 (= G218), N218 (= N219), V219 (≠ A220), D239 (= D240), I240 (≠ S241), N276 (= N286), A291 (= A301), A313 (= A323), N314 (= N324), N339 (= N349)

8kaoC Glutamate dehydrogenase-69o
42% identity, 96% coverage: 11:420/428 of query aligns to 8:410/412 of 8kaoC

query
sites
8kaoC

R

K

L

L

E

Y

R

K

A

V

L

G

Q

E

Y

L

V

L

S

G

L

L

S

D

E

S

D

E

T

T

K

I

E

D

R

V

L

L

K

S

Y

Q

P

P

K

E

A

R

T

V

L

M

I

Q

V

V

S

K

I

I

P

Q

V

I

R

R

M

S

D

S

D

D

G

G

S

K

L

L

R

R

V

T

F

F

Q

I

G

G

Y

W

R

R

V

S

R

Q

Y

H

D

N

D

S

T

A

R

L

G

G

P

P

T

Y

K

K

G
|
G

I

V

R

R

Y

Y

H

G

P

P

N

N

V

V

S

T

I

Q

D

D

E

E

V

V

K

I

S

A

L

L

A

S

F

M

W

I

M
|
M

T

T

F

W

K
|
K

C

N

A

S

V

L

S

L

L

L

P

P

F

Y

G

G

A

G

K
|
K

G

G

I

I

T

R

V

V

N

D

P

P

K

K

E

K

L

L

S

T

R

L

M

K

E

E

L

L

E

E

R

D

L

L

S

S

R

R

G

K

Y

Y

I

V

D

Q

A

L

I

I

A

H

D

K

F

Y

I

L

G

G

P

S

D

D

T

V

D

D

I

I

P

P

A
|
A

P
|
P

D
|
D

M
x
I

Y

N

T

T

N

N

P

P

M

Q

I

T

M

M

G

A

W

W

M

F

M

L

D

D

E

E

Y

Y

S

I

I

K

I

I

R

T

R

G

Q

K

L

V

S

D

P

F

A

A

V

V

I

F

T

T

G

G

K

K

P

P

V

S

S

E

I

L

G

G

S

I

L

G

G

V

R

R

N

L

T

Y

A

S

T
|
T

A

G

M

L

G

G

A

V

F

A

F

T

A

I

I

A

E

R

T

D

I

A

M

A

P

N

K

K

F

F

E

I

Y

G

T

G

P

I

E

E

K

G

T

S

T

R

V

V

A

I

I

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

A
x
V

G

G

A

F

F

F

L

T

A

A

E

K

L

F

L

L

C

S

L

E

H

M

G

G

Y

A

K

K

V

I

A

G

V

V

S

S

D
|
D

S
x
I

Q

G

G

G

I

V

Y

I

S

N

A

E

Q

N

G

G

L

I

D

D

I

-

P

-

S

-

I

-

R

-

Q

-

Y

-

K

-

E

-

V

V

N

N

K

R

Q

A

I

L

Q

E

A

V

V

V

Y

Q

C

S

K

T

D

G

S

S

V

V

C

V

N

N

I

Y

V

L

E

E

H

G

T

K

V

K

L

V

T

T

N
|
N

E

E

L

L

A

I

L

S

D

D

V

C

D

D

I

I

L

L

I

I

P

P

A

A

A
|
A

L

V

E

E

N

N

Q

V

I

I

T

N

E

K

E

F

N

N

V

A

K

P

D

K

I

V

Q

R

A

A

K

K

F

L

I

I

F

V

E

E

A

G

A
|
A

N
|
N

G

G

P

P

T

L

T

T

S

A

G

D

A

A

D

D

K

E

I

I

L

M

E

K

D

Q

R

R

G

G

V

V

Y

I

V

V

F

I

P

P

D

D

I

I

L

L

I

A

N
|
N

A

A

G

G

V
|
V

T

V

V

G

S
|
S

Y

Y

F

V

E

E

W

W

V

A

Q

N

N

N

R

R

S

S

G

G

L

G

Y

I

W

I

T

S

L

D

D

E

E

E

V

A

N

K

Q

K

K

L

L

I

K

V

A

D

K

R

M

M

V

I

A

N

E

A

T

F

E

N

N

T

I

L

W

Y

Q

D

I

F

S

H

Q

K

E

R

-

K

-

F

L

A

S

D

I

Q

S

D

M

L

R

R

T

T

A

V

A

A

Y

M

V

A

H

L

G

A

L

V

D

D

R

R

L

V

G

V

Q

G

A

A

I

M

N

K

A

A

K

R

G

G

binding 2-oxopentanoic acid: G66 (= G69), G67 (= G70), M86 (= M89), K89 (= K92), K101 (= K104), A139 (= A142), N312 (= N324), N337 (= N349), V341 (= V353), S344 (= S356)
binding nicotinamide-adenine-dinucleotide: P140 (= P143), D141 (= D144), I142 (≠ M145), T185 (= T188), F214 (= F217), G215 (= G218), N216 (= N219), V217 (≠ A220), D237 (= D240), I238 (≠ S241), N274 (= N286), A289 (= A301), A311 (= A323), N312 (= N324)

3aoeF Crystal structure of hetero-hexameric glutamate dehydrogenase from thermus thermophilus (leu bound form)
36% identity, 98% coverage: 4:421/428 of query aligns to 11:415/417 of 3aoeF

query
sites
3aoeF

S

G

L

L

F

W

D

D

D

T

A

Y

S

L

R

E

R

W

L

L

E

E

R

R

A

A

L

L

Q

K

Y

V

V

A

S

G

L

V

S

H

E

P

D

T

T

T

K

L

E

E

R
x
Y

L

L

K

A

Y

H

P

P

K

K

A

R

T

L

L

V

I

T

V

L

S

S

I

L

P

P

V

V

R

V

M

M

D

D

G

G

S

K

L

V

R

R

V

I

F

F

Q

Q

G

G

Y

Y

R

R

V

V

R

V

Y

H

D

D

D

I

T

A

R

R

G

G

P

P

T

A

K

K

G

G

I

V

R

R

Y

L

H

D

P

P

N

G

V

V

S

T

I

L

D

G

E

Q

V

T

K

A

S

G

L

L

A

A

F

A

W

W

M

M

T

T

F

L

K

K

C

A

A

A

V

V

V

Y

S

D

L

L

P

P

F

F

G

G

A

A

K
x
A

G

G

I

I

T

A

V

V

N

D

P

P

K

K

E

G

L

L

S

S

R

P

M

Q

E

E

L

L

E

E

R

R

L

L

S

V

R

R

G

R

Y

Y

I

T

D

A

A

E

I

L

A

V

D

G

F

L

I

I

G

G

P

P

D

D

T

S

D

D

I

I

P

L

A

G

P

P

D
|
D

M

L

Y

G

T

A

N

D

P

Q

M

Q

I

V

M

M

G

A

W

W

M

I

M

M

D

D

E

T

Y

Y

S

S

I

M

I

T

R

V

R

G

Q

S

L

T

S

V

P

P

A

G

V

V

I

V

T

T

G

G

K

K

P

P

V

H

S

A

I

L

G

G

S

S

L

E

G

G

R

R

N

D

T

D

A

A

T

A

A

G

M

L

G

G

A

A

F

L

A

V

I

L

E

E

T

A

I

L

M

A

P

K

K

R

F

R

E

G

Y

L

T

D

P

L

E

R

K

G

T

A

T

R

V

V

A

V

I

V

Q

Q

G

G

F

L

G

G

N

Q

A

V

G

G

A

A

F

A

L

V

A

A

E

L

L

H

L

A

C

E

L

R

H

L

G

G

Y

M

K

R

V

V

A

A

V

V

S

A

D

T

S

S

Q

M

G

G

I

M

Y

Y

S

A

A

P

Q

E

G

G

L

L

D

D

I

V

P

A

S

E

I

V

R

L

Q

S

Y

A

K

Y

E

E

V

A

N

T

K

G

Q

S

I

L

Q

P

A

-

V

-

Y

-

C

-

K

-

D

-

S

-

V

-

C

-

N

-

I

-

V

-

E

-

H

R

T

L

V

D

L

L

T

A

N

P

E

E

L

V

L

F

A

G

L

L

D

E

V

A

D

E

I

V

L

L

I

V

P

L

A

A

L

R

E

E

N

G

Q

A

I

L

T

D

E

G

E

D

N

R

V

A

K

R

D

Q

I

V

Q

Q

A

A

K

Q

F

A

I

V

F

V

E

E

A

V

A

A

N

N

G

F

P

G

T

L

T

N

S

P

G

E

A

A

D

E

K

A

I

Y

L

L

E

L

D

G

R

K

G

G

V

A

Y

L

V

V

F

V

P

P

D

D

I

L

L

L

I

S

N

G

A

G

G

G

V

L

T

L

V

A

S

S

Y

Y

F

L

E

E

W

W

V

V

Q

Q

N

D

R

L

S

N

G

M

L

F

Y

F

W

W

T

S

L

P

D

E

E

E

V

V

N

R

Q

E

K

R

L

F

K

E

A

T

K

R

M

V

V

A

A

R

E

V

T

V

E

D

N

A

I

V

W

C

Q

R

I

R

S

A

Q

E

E

R

L

G

S

G

I

L

S

D

M

L

R

R

T

M

A

G

A

A

Y

L

V

A

H

L

G

A

L

L

D

E

R

R

L

L

G

D

Q

E

A

A

I

T

N

R

A

L

K

R

G
|
G

T
x
V

active site: A111 (≠ K104), D151 (= D144)
binding leucine: Y36 (≠ R29), G414 (= G420), V415 (≠ T421)

Sites not aligning to the query:

binding leucine: 416

6dhlA Bovine glutamate dehydrogenase complexed with epicatechin-3-gallate (ecg) (see paper)
41% identity, 85% coverage: 1:363/428 of query aligns to 7:383/496 of 6dhlA

query
sites
6dhlA

M

M

S

V

D

E

S

G

L

F

F

F

D

D

D

R

A

G

S

A

R

S

R

I

L

V

E

E

R

D

A

K

L

L

Q

V

Y

E

V

D

S

L

L

K

S

T

E

R

D

E

T

T

K

E

E

E

R

Q

-

K

-

R

-

N

-

R

-

V

-

R

-

G

-

I

-

L

-

R

-

I

L

I

K

K

Y

P

P

C

K

N

A

H

T

V

L

L

I

S

V

L

S

S

I

F

P

P

V

I

R

R

M

R

D

D

G

G

S

S

L

W

R

E

V

V

F

I

Q

E

G

G

Y

Y

R

R

V

A

R

Q

Y

H

D

S

D

Q

T
x
H

R
|
R

G

T

P

P

T

C

K

K

G

G

I

I

R

R

Y

Y

H

S

P

T

N

D

V

V

S

S

I

V

D

D

E

E

V

V

K

K

S

A

L

L

A

A

F

S

W

L

M

M

T

T

F

Y

K

K

C
|
C

A

A

V

V

S
x
D

L
x
V

P

P

F

F

G

G

A

A

K

K

G

A

G

G

I

V

T

K

V

I

N

N

P

P

K

K

E

N

L

Y

S

T

R

D

M

N

E

E

L

L

E

E

R

K

L

I

S

T

R

R

G

R

Y

F

I

T

D

M

A

E

I

L

A

A

D

K

-

K

-

G

F

F

I

I

G

G

P

P

D

G

T

V

D

D

I

V

P

P

A

A

P

P

D

D

M

M

Y

S

T

T

N

G

P

E

M

R

I

E

M

M

G

S

W

W

M

I

M

A

D

D

E

T

Y

Y

S

A

I

S

I

T

R

I

R

G

Q

H

L

Y

-

D

-

I

-

N

S

A

P

H

A

A

V

C

I

V

T

T

G

G

K

K

P

P

V

I

S

S

I

Q

G

G

S

I

L

H

G

G

R

R

N

I

T

S

A

A

T

T

A

G

M

R

G

G

A

V

F

F

F

H

A

G

I

I

E

E

T

N

I

F

M

I

P

N

K

E

F

A

E

S

Y

Y

-

M

-

S

-

I

-

L

-

G

-

M

T

T

P

P

-

G

-

F

E

G

K

D

T

K

T

T

V

F

A

A

I

V

Q

Q

G

G

F

F

G

G

N

N

A

V

G

G

A

L

F

H

L

S

A

M

E

R

L

Y

L

L

C

H

L

R

H

F

G

G

Y

A

K

K

V

C

V

V

A

A

V

V

S

G

D

E

S

S

Q

D

G

G

G

S

I

I

Y

W

S

N

A

P

Q

D

G

G

L

I

D

D

I

P

P

K

S

E

I

L

R

E

Q

D

Y

F

K

K

E

L

V

Q

N

H

K

G

Q

T

I

I

Q

L

A

G

V

F

Y

-

C

-

K

-

D

-

S

P

V

K

C

A

N

K

I

I

V

Y

E

E

H

G

T

S

V

I

L

-

T

-

N

-

E

-

L

-

L

L

A

E

L

V

D

D

V

C

D

D

I

I

L

L

I

I

P

P

A

A

L

S

E

E

N

K

Q

Q

I

L

T

T

E

K

E

S

N

N

V

A

K

P

D

R

I

V

Q

K

A

A

K

K

F

I

I

I

F

A

E

E

A

G

A

A

N

N

G

G

P

P

T

T

S

P

G

E

A

A

D

D

K

K

I

I

L

F

E

L

D

E

R

R

G

N

V

I

Y

M

V

V

F

I

P

P

D

D

I

L

L

Y

I

L

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

L

Q
x
K

N

N

binding (2R,3S)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate: H80 (≠ T63), R81 (= R64), C110 (= C93), D114 (≠ S97), V115 (≠ L98), K382 (≠ Q362)

Sites not aligning to the query:

binding (2R,3S)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate: 388, 391, 454, 483

Query Sequence

>WP_011613632.1 NCBI__GCF_000014265.1:WP_011613632.1
MSDSLFDDASRRLERALQYVSLSEDTKERLKYPKATLIVSIPVRMDDGSLRVFQGYRVRY
DDTRGPTKGGIRYHPNVSIDEVKSLAFWMTFKCAVVSLPFGGAKGGITVNPKELSRMELE
RLSRGYIDAIADFIGPDTDIPAPDMYTNPMIMGWMMDEYSIIRRQLSPAVITGKPVSIGG
SLGRNTATAMGAFFAIETIMPKFEYTPEKTTVAIQGFGNAGAFLAELLCLHGYKVVAVSD
SQGGIYSAQGLDIPSIRQYKEVNKQIQAVYCKDSVCNIVEHTVLTNEELLALDVDILIPA
ALENQITEENVKDIQAKFIFEAANGPTTSGADKILEDRGVYVFPDILINAGGVTVSYFEW
VQNRSGLYWTLDEVNQKLKAKMVAETENIWQISQELSISMRTAAYVHGLDRLGQAINAKG
TRAFYAKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory