SitesBLAST
Comparing WP_011650233.1 NCBI__GCF_000009265.1:WP_011650233.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6gmcA 1.2 a resolution structure of human hydroxyacid oxidase 1 bound with fmn and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole
38% identity, 98% coverage: 6:379/382 of query aligns to 7:359/360 of 6gmcA
- active site: Y132 (= Y132), D160 (= D160), H258 (= H278)
- binding 5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole-4-carboxylate: Y26 (= Y27), M82 (≠ L83), W110 (≠ L111), Y132 (= Y132), R167 (= R167), F191 (≠ V191), L203 (≠ I223), Y206 (= Y226), H258 (= H278), R261 (= R281)
- binding flavin mononucleotide: Y26 (= Y27), Y27 (≠ I28), A79 (≠ P80), T80 (= T81), A81 (= A82), S108 (= S109), Q130 (= Q130), Y132 (= Y132), T158 (= T158), K234 (= K254), H258 (= H278), G259 (= G279), R261 (= R281), D289 (= D309), G290 (= G310), R293 (≠ Q313), G312 (= G332), R313 (= R333)
1al7A Three-dimensional structures of glycolate oxidase with bound active- site inhibitors (see paper)
37% identity, 98% coverage: 5:378/382 of query aligns to 2:345/350 of 1al7A
- active site: S106 (= S109), Y129 (= Y132), T155 (= T158), D157 (= D160), K221 (= K254), H245 (= H278)
- binding flavin mononucleotide: Y24 (= Y27), Y25 (≠ I28), P77 (= P80), T78 (= T81), A79 (= A82), S106 (= S109), Q127 (= Q130), T155 (= T158), K221 (= K254), H245 (= H278), R248 (= R281), D276 (= D309), G277 (= G310), R280 (≠ Q313), G299 (= G332), R300 (= R333)
- binding 4-carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole: Y24 (= Y27), W108 (≠ L111), Y129 (= Y132), R164 (= R167), F172 (= F175), I198 (≠ L231), H245 (= H278), R248 (= R281)
P05414 Glycolate oxidase; GAO; GOX; Short chain alpha-hydroxy acid oxidase; EC 1.1.3.15 from Spinacia oleracea (Spinach) (see 4 papers)
37% identity, 98% coverage: 5:378/382 of query aligns to 2:354/369 of P05414
- Y24 (= Y27) mutation to F: 10-fold decrease in affinity for glycolate.
- PTA 77:79 (= PTA 80:82) binding FMN
- S106 (= S109) binding FMN
- W108 (≠ L111) mutation to S: 100-fold decrease in affinity for glycolate and 500-fold decrease in activity.
- QLY 127:129 (≠ QFY 130:132) binding FMN
- T155 (= T158) binding FMN
- K230 (= K254) binding FMN
- S252 (= S276) binding FMN
- DGGVR 285:289 (≠ DGGVQ 309:313) binding FMN
- GR 308:309 (= GR 332:333) binding FMN
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
5zbmB Structure of glycolate oxidase containing fmn from nicotiana benthamiana (see paper)
37% identity, 98% coverage: 5:378/382 of query aligns to 1:348/353 of 5zbmB
- active site: Y128 (= Y132), D156 (= D160), H248 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), P76 (= P80), T77 (= T81), W107 (≠ L111), Q126 (= Q130), Y128 (= Y132), T154 (= T158), K224 (= K254), H248 (= H278), G249 (= G279), R251 (= R281), D279 (= D309), G280 (= G310), R283 (≠ Q313), G302 (= G332), R303 (= R333)
Q9UJM8 2-Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GO; GOX; Glyoxylate oxidase; EC 1.1.3.15; EC 1.2.3.5 from Homo sapiens (Human) (see 2 papers)
38% identity, 97% coverage: 6:377/382 of query aligns to 7:363/370 of Q9UJM8
- Y26 (= Y27) binding glyoxylate
- ATA 79:81 (≠ PTA 80:82) binding FMN
- S108 (= S109) binding FMN
- Q130 (= Q130) binding FMN
- Y132 (= Y132) binding glyoxylate
- T158 (= T158) binding FMN
- R167 (= R167) binding glyoxylate
- K236 (= K254) binding FMN
- S258 (= S276) binding FMN
- H260 (= H278) binding glyoxylate
- R263 (= R281) binding glyoxylate
- DGGVR 291:295 (≠ DGGVQ 309:313) binding FMN
- GR 314:315 (= GR 332:333) binding FMN
2rduA Crystal structure of human glycolate oxidase in complex with glyoxylate (see paper)
38% identity, 98% coverage: 6:379/382 of query aligns to 5:359/360 of 2rduA
- active site: S106 (= S109), Y130 (= Y132), T156 (= T158), D158 (= D160), K234 (= K254), H258 (= H278)
- binding flavin mononucleotide: Y24 (= Y27), Y25 (≠ I28), A77 (≠ P80), T78 (= T81), A79 (= A82), S106 (= S109), Q128 (= Q130), Y130 (= Y132), T156 (= T158), K234 (= K254), H258 (= H278), G259 (= G279), R261 (= R281), D289 (= D309), G290 (= G310), G291 (= G311), R293 (≠ Q313), G312 (= G332), R313 (= R333)
- binding glyoxylic acid: Y24 (= Y27), W108 (≠ L111), Y130 (= Y132), R165 (= R167), H258 (= H278), R261 (= R281)
6gmbA Structure of human hydroxyacid oxidase 1 bound with fmn and glycolate
38% identity, 98% coverage: 6:379/382 of query aligns to 7:361/362 of 6gmbA
- active site: Y132 (= Y132), D160 (= D160), H260 (= H278)
- binding flavin mononucleotide: Y26 (= Y27), Y27 (≠ I28), A79 (≠ P80), T80 (= T81), A81 (= A82), S108 (= S109), Q130 (= Q130), Y132 (= Y132), K236 (= K254), H260 (= H278), G261 (= G279), R263 (= R281), D291 (= D309), G292 (= G310), G293 (= G311), R295 (≠ Q313), G314 (= G332), R315 (= R333)
- binding glycolic acid: Y26 (= Y27), W110 (≠ L111), Y132 (= Y132), R167 (= R167), H260 (= H278), R263 (= R281)
5qigA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1407672867
38% identity, 98% coverage: 6:379/382 of query aligns to 4:358/359 of 5qigA
- active site: Y129 (= Y132), D157 (= D160), H257 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), T155 (= T158), K233 (= K254), H257 (= H278), G258 (= G279), R260 (= R281), D288 (= D309), G289 (= G310), G290 (= G311), R292 (≠ Q313), G311 (= G332), R312 (= R333)
- binding 2-cyclopropyl-1~{H}-imidazole-4-carboxamide: R223 (≠ V244), G248 (= G269), F320 (≠ A341), K324 (≠ P345), D328 (≠ R349)
Sites not aligning to the query:
5qifA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z31792168
38% identity, 98% coverage: 6:379/382 of query aligns to 4:358/359 of 5qifA
- active site: Y129 (= Y132), D157 (= D160), H257 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), K233 (= K254), H257 (= H278), G258 (= G279), R260 (= R281), D288 (= D309), G289 (= G310), R292 (≠ Q313), G311 (= G332), R312 (= R333)
- binding 2-cyclohexyl-~{N}-pyridin-3-yl-ethanamide: R223 (≠ V244), R224 (= R245), S227 (≠ G248)
2rdwA Crystal structure of human glycolate oxidase in complex with sulfate (see paper)
38% identity, 98% coverage: 6:379/382 of query aligns to 4:358/359 of 2rdwA
- active site: S105 (= S109), Y129 (= Y132), T155 (= T158), D157 (= D160), K233 (= K254), H257 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), Q127 (= Q130), Y129 (= Y132), T155 (= T158), K233 (= K254), H257 (= H278), G258 (= G279), R260 (= R281), D288 (= D309), G289 (= G310), R292 (≠ Q313), G311 (= G332), R312 (= R333)
- binding sulfate ion: Y23 (= Y27), W107 (≠ L111), R164 (= R167), H257 (= H278), R260 (= R281)
Q8Z0C8 L-lactate oxidase; LOX; Glyoxylate oxidase; No-LOX; EC 1.1.3.-; EC 1.2.3.5 from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
39% identity, 97% coverage: 3:373/382 of query aligns to 4:358/365 of Q8Z0C8
- M82 (≠ T81) mutation to T: Increases oxidation activity with both L-lactate and glycolate. Shows a 6-fold decrease in the L-lactate/glycolate oxidase activity ratio.
- L112 (= L111) mutation to W: Impairs oxidation of L-lactate. Shows a 2-fold decrease in the L-lactate/glycolate oxidase activity ratio.
- F212 (= F227) mutation to V: Impairs oxidation of L-lactate. Shows a 27-fold decrease in the L-lactate/glycolate oxidase activity ratio.
7r4nA Structure of human hydroxyacid oxidase 1 bound with 5-bromo-n-methyl- 1h-indazole-3-carboxamide
37% identity, 98% coverage: 6:379/382 of query aligns to 6:351/352 of 7r4nA
- binding 5-bromanyl-N-methyl-1H-indazole-3-carboxamide: Y25 (= Y27), A80 (= A82), M81 (≠ L83), W109 (≠ L111), Y131 (= Y132), R166 (= R167), M182 (≠ L183), H250 (= H278), R253 (= R281)
- binding flavin mononucleotide: Y25 (= Y27), Y26 (≠ I28), A78 (≠ P80), T79 (= T81), A80 (= A82), S107 (= S109), W109 (≠ L111), Q129 (= Q130), Y131 (= Y132), T157 (= T158), K226 (= K254), H250 (= H278), G251 (= G279), R253 (= R281), D281 (= D309), G282 (= G310), R285 (≠ Q313), G304 (= G332), R305 (= R333)
5qieA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z2856434894
37% identity, 98% coverage: 6:379/382 of query aligns to 4:352/353 of 5qieA
- active site: Y129 (= Y132), D157 (= D160), H251 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), K227 (= K254), H251 (= H278), G252 (= G279), R254 (= R281), D282 (= D309), G283 (= G310), R286 (≠ Q313), G305 (= G332), R306 (= R333)
- binding ~{N},~{N}-dimethyl-4-[(propan-2-ylamino)methyl]aniline: D322 (≠ R349)
Sites not aligning to the query:
5qihA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z2697514548
37% identity, 98% coverage: 6:379/382 of query aligns to 4:343/344 of 5qihA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), K218 (= K254), H242 (= H278), G243 (= G279), R245 (= R281), D273 (= D309), G274 (= G310), R277 (≠ Q313), G296 (= G332), R297 (= R333)
- binding 1-methylindazole-3-carboxamide: Y23 (= Y27), A78 (= A82), M79 (≠ L83), W107 (≠ L111), Y129 (= Y132), M180 (= M216), L187 (≠ I223), H242 (= H278)
5qidA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1787627869
37% identity, 98% coverage: 6:379/382 of query aligns to 4:343/344 of 5qidA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), K218 (= K254), H242 (= H278), G243 (= G279), R245 (= R281), D273 (= D309), G274 (= G310), R277 (≠ Q313), G296 (= G332), R297 (= R333)
- binding 5-chloranyl-~{N}-methyl-~{N}-[[(3~{S})-oxolan-3-yl]methyl]pyrimidin-4-amine: T57 (≠ V61), E320 (≠ T356), R323 (≠ E359)
5qicA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z30620520
37% identity, 98% coverage: 6:379/382 of query aligns to 4:343/344 of 5qicA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H278)
- binding flavin mononucleotide: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), K218 (= K254), H242 (= H278), G243 (= G279), R245 (= R281), D273 (= D309), G274 (= G310), R277 (≠ Q313), G296 (= G332), R297 (= R333)
- binding cyclopropyl-[4-(4-fluorophenyl)piperazin-1-yl]methanone: Y131 (≠ H134), K132 (= K135), V136 (≠ L139), M180 (= M216), F183 (≠ G219), Y190 (= Y226), K193 (≠ E229), A194 (≠ M230)
5qibA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with fmopl000388a
37% identity, 98% coverage: 6:379/382 of query aligns to 4:343/344 of 5qibA
- active site: Y129 (= Y132), D157 (= D160), H242 (= H278)
- binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: Y23 (= Y27), Y24 (≠ I28), A76 (≠ P80), T77 (= T81), A78 (= A82), S105 (= S109), Q127 (= Q130), Y129 (= Y132), K218 (= K254), H242 (= H278), G243 (= G279), R245 (= R281), D273 (= D309), G274 (= G310), G275 (= G311), R277 (≠ Q313), G296 (= G332), R297 (= R333)
- binding 5-(cyclohexylamino)pyrimidine-2,4(1H,3H)-dione: Y23 (= Y27), M79 (≠ L83), W107 (≠ L111), Y129 (= Y132), R164 (= R167), M180 (= M216), L187 (≠ I223), H242 (= H278), R245 (= R281)
1al8A Three-dimensional structure of glycolate oxidase with bound active- site inhibitors (see paper)
36% identity, 98% coverage: 5:378/382 of query aligns to 2:339/344 of 1al8A
- active site: S106 (= S109), Y129 (= Y132), T155 (= T158), D157 (= D160), K215 (= K254), H239 (= H278)
- binding 3-decyl-2,5-dioxo-4-hydroxy-3-pyrroline: Y24 (= Y27), W108 (≠ L111), Y129 (= Y132), F166 (= F205), H239 (= H278), R242 (= R281)
- binding flavin mononucleotide: Y25 (≠ I28), P77 (= P80), T78 (= T81), A79 (= A82), S106 (= S109), Q127 (= Q130), Y129 (= Y132), T155 (= T158), K215 (= K254), H239 (= H278), R242 (= R281), D270 (= D309), G271 (= G310), R274 (≠ Q313), G293 (= G332), R294 (= R333)
6a24A The crystal structure of mandelate oxidase with 3-fluoropyruvate (see paper)
37% identity, 98% coverage: 8:380/382 of query aligns to 3:352/354 of 6a24A
- active site: Y126 (= Y132), D154 (= D160), H250 (= H278)
- binding flavin mononucleotide: P75 (= P80), V76 (≠ T81), A77 (= A82), Q124 (= Q130), Y126 (= Y132), T152 (= T158), K226 (= K254), H250 (= H278), G251 (= G279), R253 (= R281), D281 (= D309), G282 (= G310), G283 (= G311), R285 (≠ Q313), G304 (= G332), R305 (= R333)
- binding pyruvic acid: R161 (= R167), H250 (= H278), R253 (= R281)
5zzqA The crystal structure of mandelate oxidase with (s)-4-hydroxymandelic acid (see paper)
37% identity, 98% coverage: 8:380/382 of query aligns to 3:352/355 of 5zzqA
- active site: Y126 (= Y132), D154 (= D160), H250 (= H278)
- binding flavin mononucleotide: P75 (= P80), V76 (≠ T81), A77 (= A82), Q124 (= Q130), Y126 (= Y132), T152 (= T158), K226 (= K254), H250 (= H278), R253 (= R281), D281 (= D309), G283 (= G311), R285 (≠ Q313), G304 (= G332), R305 (= R333)
- binding (2S)-hydroxy(4-hydroxyphenyl)ethanoic acid: L106 (= L111), Y126 (= Y132), M158 (≠ G164), R161 (= R167), T200 (≠ F227), F204 (≠ L231), H250 (= H278), R253 (= R281)
Query Sequence
>WP_011650233.1 NCBI__GCF_000009265.1:WP_011650233.1
MRLTDCYNFHDFRRMAKRRLPGPIFDYIDGGADDEVTYRRNTAAFEACDLVPDVLRGVAD
VDMSVTVMGQKLAMPVYCSPTALQRLFHHQGERAVAAAAAKHGTMFGVSSLGTISLEEAR
QISNGPQVYQFYFHKDRGLNREMMARAKNAGVQAMMLTVDSITGGNRERDKRTGFAIPFK
LNLAGMTQFAVKPSWAIDWLTHERFRLPQLENHVKMDGGALSISRYFTEMLDPSMSWDDV
AEMVREWGGPFCLKGIMSVEDAKRAAEIGCSGIVLSNHGGRQLDGSRSAFDQLAEIVDAV
GDRIDVMMDGGVQRGTHVLKALSLGAKAVGLGRYYLFPLAAAGQPGVERALETMRTEIER
GMKLMGCTSVSQLTRRNLRFRS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory