SitesBLAST
Comparing WP_011650388.1 NCBI__GCF_000009265.1:WP_011650388.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
40% identity, 96% coverage: 8:346/354 of query aligns to 5:346/362 of 3bptA
- active site: G67 (= G70), P84 (≠ A87), R88 (= R91), G115 (= G118), G118 (= G121), E138 (= E141), D146 (= D149)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G69), G67 (= G70), I69 (= I72), E90 (= E93), G114 (= G117), G115 (= G118), E138 (= E141), D146 (= D149), V147 (= V150)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A28), L26 (= L29), A28 (≠ S31), G66 (= G69), G67 (= G70), I69 (= I72), P137 (= P140), I141 (= I144), L319 (≠ V319)
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
40% identity, 98% coverage: 7:354/354 of query aligns to 1:337/340 of 4hdtA
- active site: G64 (= G70), I69 (≠ L75), W84 (= W90), Y88 (≠ F94), G112 (= G118), G115 (= G121), E135 (= E141), P142 (= P148), D143 (= D149), R283 (≠ G296)
- binding zinc ion: H28 (≠ L34), E42 (≠ A48), E57 (= E63), E79 (≠ L85), H93 (≠ L99), H185 (≠ S191)
Sites not aligning to the query:
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 97% coverage: 7:349/354 of query aligns to 8:357/378 of Q9LKJ1
- G70 (= G70) mutation to S: Loss of activity.
- E142 (= E141) mutation to A: Loss of activity.
- D150 (= D149) mutation to G: Reduced activity.
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
35% identity, 49% coverage: 10:184/354 of query aligns to 6:178/260 of 2hw5C
- active site: A68 (≠ G70), M73 (≠ L75), S83 (≠ A86), L87 (≠ W90), G111 (= G118), E114 (≠ G121), P133 (= P140), E134 (= E141), T139 (≠ Y146), P141 (= P148), G142 (≠ D149)
- binding crotonyl coenzyme a: K26 (≠ R27), A27 (= A28), L28 (= L29), A30 (≠ S31), K62 (≠ R64), I70 (= I72), F109 (≠ M116)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
33% identity, 49% coverage: 10:184/354 of query aligns to 6:176/258 of 1mj3A
- active site: A68 (≠ G70), M73 (≠ L75), S83 (≠ L85), L85 (≠ A87), G109 (= G118), E112 (≠ G121), P131 (= P140), E132 (= E141), T137 (≠ Y146), P139 (= P148), G140 (≠ D149)
- binding hexanoyl-coenzyme a: K26 (≠ R27), A27 (= A28), L28 (= L29), A30 (≠ S31), A66 (= A68), G67 (= G69), A68 (≠ G70), D69 (= D71), I70 (= I72), G109 (= G118), P131 (= P140), E132 (= E141), L135 (≠ I144), G140 (≠ D149)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
33% identity, 49% coverage: 10:184/354 of query aligns to 6:178/260 of 1dubA
- active site: A68 (≠ G70), M73 (≠ L75), S83 (≠ A81), L87 (= L85), G111 (= G118), E114 (≠ G121), P133 (= P140), E134 (= E141), T139 (≠ Y146), P141 (= P148), G142 (≠ D149)
- binding acetoacetyl-coenzyme a: K26 (≠ R27), A27 (= A28), L28 (= L29), A30 (≠ S31), A66 (= A68), A68 (≠ G70), D69 (= D71), I70 (= I72), Y107 (≠ I114), G110 (= G117), G111 (= G118), E114 (≠ G121), P133 (= P140), E134 (= E141), L137 (≠ I144), G142 (≠ D149)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
33% identity, 49% coverage: 10:184/354 of query aligns to 36:208/290 of P14604
- E144 (≠ G121) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E141) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
33% identity, 49% coverage: 10:184/354 of query aligns to 4:176/258 of 1ey3A
- active site: A66 (≠ G70), M71 (≠ L75), S81 (≠ A81), L85 (= L85), G109 (= G118), E112 (≠ G121), P131 (= P140), E132 (= E141), T137 (≠ Y146), P139 (= P148), G140 (≠ D149)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ R27), L26 (= L29), A28 (≠ S31), A64 (= A68), G65 (= G69), A66 (≠ G70), D67 (= D71), I68 (= I72), L85 (= L85), W88 (= W90), G109 (= G118), P131 (= P140), L135 (≠ I144), G140 (≠ D149)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
33% identity, 49% coverage: 10:184/354 of query aligns to 5:172/254 of 2dubA
- active site: A67 (≠ G70), M72 (≠ L75), S82 (= S88), G105 (= G118), E108 (≠ G121), P127 (= P140), E128 (= E141), T133 (≠ Y146), P135 (= P148), G136 (≠ D149)
- binding octanoyl-coenzyme a: K25 (≠ R27), A26 (= A28), L27 (= L29), A29 (≠ S31), A65 (= A68), A67 (≠ G70), D68 (= D71), I69 (= I72), K70 (≠ R73), G105 (= G118), E108 (≠ G121), P127 (= P140), E128 (= E141), G136 (≠ D149), A137 (≠ V150)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
29% identity, 50% coverage: 10:185/354 of query aligns to 24:201/1413 of 6z1pBI
- active site: T85 (≠ G70), S134 (≠ G118), E157 (= E141), D165 (= D149)
- binding : Y41 (≠ R27), K42 (≠ A28), Q43 (≠ L29), T45 (≠ S31), D47 (≠ T33), H49 (≠ P35), K83 (≠ A68), T85 (≠ G70), D86 (= D71), F87 (≠ I72), K88 (≠ R73), K92 (≠ E77), L130 (≠ I114), K152 (≠ R136)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
29% identity, 49% coverage: 8:179/354 of query aligns to 4:170/255 of 3q0jC
- active site: A65 (≠ G70), M70 (≠ L75), T80 (≠ A86), F84 (≠ W90), G108 (= G118), E111 (≠ G121), P130 (= P140), E131 (= E141), V136 (≠ Y146), P138 (= P148), G139 (≠ D149)
- binding acetoacetyl-coenzyme a: Q23 (≠ R27), A24 (= A28), L25 (= L29), A27 (≠ S31), A63 (= A68), G64 (= G69), A65 (≠ G70), D66 (= D71), I67 (= I72), K68 (≠ R73), M70 (≠ L75), F84 (≠ W90), G107 (= G117), G108 (= G118), E111 (≠ G121), P130 (= P140), E131 (= E141), P138 (= P148), G139 (≠ D149), M140 (≠ V150)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 49% coverage: 8:179/354 of query aligns to 4:170/255 of 3q0gC
- active site: A65 (≠ G70), M70 (≠ L75), T80 (≠ A86), F84 (≠ W90), G108 (= G118), E111 (≠ G121), P130 (= P140), E131 (= E141), V136 (≠ Y146), P138 (= P148), G139 (≠ D149)
- binding coenzyme a: L25 (= L29), A63 (= A68), I67 (= I72), K68 (≠ R73), Y104 (≠ I114), P130 (= P140), E131 (= E141), L134 (≠ I144)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 49% coverage: 8:179/354 of query aligns to 3:165/250 of 3q0gD
- active site: A64 (≠ G70), M69 (≠ L75), T75 (≠ A81), F79 (≠ L85), G103 (= G118), E106 (≠ G121), P125 (= P140), E126 (= E141), V131 (≠ Y146), P133 (= P148), G134 (≠ D149)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
29% identity, 49% coverage: 8:179/354 of query aligns to 3:169/256 of 3h81A
- active site: A64 (≠ G70), M69 (≠ L75), T79 (≠ A86), F83 (≠ W90), G107 (= G118), E110 (≠ G121), P129 (= P140), E130 (= E141), V135 (≠ Y146), P137 (= P148), G138 (≠ D149)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
31% identity, 47% coverage: 13:179/354 of query aligns to 9:172/259 of 5zaiC
- active site: A65 (≠ G70), F70 (≠ L75), S82 (≠ W90), R86 (≠ F94), G110 (= G118), E113 (≠ G121), P132 (= P140), E133 (= E141), I138 (≠ Y146), P140 (= P148), G141 (≠ D149)
- binding coenzyme a: K24 (≠ A28), L25 (= L29), A63 (= A68), G64 (= G69), A65 (≠ G70), D66 (= D71), I67 (= I72), P132 (= P140), R166 (≠ D173)
Sites not aligning to the query:
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
28% identity, 64% coverage: 29:253/354 of query aligns to 32:245/707 of 1wdmA
Sites not aligning to the query:
- active site: 430, 451, 463, 501
- binding acetyl coenzyme *a: 297, 459, 501, 534, 652, 658
- binding nicotinamide-adenine-dinucleotide: 321, 322, 324, 325, 344, 401, 403, 428, 430, 454
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
28% identity, 64% coverage: 29:253/354 of query aligns to 32:245/715 of 1wdlA
Sites not aligning to the query:
- active site: 430, 451, 463, 501
- binding nicotinamide-adenine-dinucleotide: 322, 324, 325, 344, 345, 400, 401, 403, 428, 429, 430
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
28% identity, 64% coverage: 29:253/354 of query aligns to 32:245/715 of P28793
Sites not aligning to the query:
- 297 binding substrate
- 325 binding NAD(+)
- 344 binding NAD(+)
- 401:403 binding NAD(+)
- 408 binding NAD(+)
- 430 binding NAD(+)
- 454 binding NAD(+)
- 501 binding substrate
- 660 binding substrate
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 45% coverage: 5:163/354 of query aligns to 8:153/257 of 6slbAAA
- active site: Q64 (≠ G70), F69 (≠ L75), L80 (≠ W90), N84 (≠ F94), A108 (≠ G118), S111 (≠ G121), A130 (≠ P140), F131 (≠ E141), L136 (≠ Y146), P138 (= P148), D139 (= D149)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R64), A62 (= A68), Q64 (≠ G70), D65 (= D71), L66 (≠ I72), Y76 (≠ A86), A108 (≠ G118), F131 (≠ E141), D139 (= D149)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
30% identity, 45% coverage: 5:163/354 of query aligns to 5:141/245 of 6slaAAA
- active site: Q61 (≠ G70), L68 (≠ W90), N72 (≠ F94), A96 (≠ G118), S99 (≠ G121), A118 (≠ P140), F119 (≠ E141), L124 (≠ Y146), P126 (= P148), N127 (≠ D149)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L29), A59 (= A68), Q61 (≠ G70), D62 (= D71), L63 (≠ I72), L68 (≠ W90), Y71 (≠ E93), A94 (≠ M116), G95 (= G117), A96 (≠ G118), F119 (≠ E141), I122 (= I144), L124 (≠ Y146), N127 (≠ D149)
Sites not aligning to the query:
Query Sequence
>WP_011650388.1 NCBI__GCF_000009265.1:WP_011650388.1
MQDGERQDEVIIERRGTAGVIRLNRPRALNSLTLPMIRTITGALHAFARDSEVASVVMMG
EGERGFCAGGDIRALHESARAGDGLAASFWREEFRLNHLIASYPKPYVALMDGITMGGGV
GLSSHGRHRIVTERTRLAMPETGIGYVPDVGATWLLPRGPGEAGTWLGLTGLDIGAADAI
HARLADLQIASSLLGETIDALSDLPRASSSGDVDAVLQALSEPQGESRLKQNAATIDRAF
RFDSVEEILAALAEEEGEFAAETCRVMLTRSPTSLKLALRLLRAGRRSASLAECLGRELG
ACLQMLDNPDFFEGIRAAVIDKDRNPQWSPASVEAVGASTVEQFLKPAEPPLSL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory