SitesBLAST
Comparing WP_011650428.1 NCBI__GCF_000009265.1:WP_011650428.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
52% identity, 100% coverage: 1:336/336 of query aligns to 4:342/343 of 4ej6A
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H61 (= H58), E62 (= E59), C91 (= C88), C94 (= C91), C97 (= C94), C105 (= C102), R109 (≠ T106), P147 (= P144), C151 (= C148), K337 (= K331)
- binding zinc ion: C91 (= C88), C94 (= C91), C97 (= C94), C105 (= C102)
4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
52% identity, 100% coverage: 1:336/336 of query aligns to 4:342/342 of 4ejmA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H61 (= H58), E62 (= E59), C91 (= C88), C94 (= C91), C97 (= C94), C105 (= C102), R109 (≠ T106), P147 (= P144), C151 (= C148), K337 (= K331)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G167), G172 (= G169), V173 (= V170), I174 (= I171), T194 (= T191), R195 (= R192), Q196 (= Q193), K199 (≠ R196), C240 (= C234), E245 (≠ D239), T246 (= T240), L263 (≠ F257), V265 (= V259), I291 (≠ L285)
- binding zinc ion: C91 (= C88), C94 (= C91), C97 (= C94), C105 (= C102)
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
31% identity, 93% coverage: 6:317/336 of query aligns to 8:324/347 of 5vm2A
- active site: C39 (= C37), G40 (= G38), S41 (= S39), H44 (= H42), H65 (= H58), E66 (= E59), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102), D113 (≠ T106), P153 (= P144), G157 (≠ C148)
- binding magnesium ion: H65 (= H58), E66 (= E59), E152 (= E143)
- binding zinc ion: C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102)
Sites not aligning to the query:
4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
35% identity, 94% coverage: 1:316/336 of query aligns to 1:325/346 of 4cpdA
- active site: C38 (= C37), G39 (= G38), S40 (= S39), H43 (= H42), H59 (= H58), E60 (= E59), C89 (= C88), C92 (= C91), C95 (= C94), C103 (= C102), G107 (≠ T106), D152 (≠ E143), T156 (≠ C147)
- binding nicotinamide-adenine-dinucleotide: G39 (= G38), S40 (= S39), T156 (≠ C147), G178 (= G169), P179 (≠ V170), V180 (≠ I171), D200 (≠ T191), R201 (= R192), R205 (= R196), A243 (≠ C234), V244 (≠ A235), V266 (≠ F257), V268 (= V259), L292 (≠ Y284), A293 (≠ L285)
- binding zinc ion: C38 (= C37), H59 (= H58), C89 (= C88), C92 (= C91), C95 (= C94), C103 (= C102), D152 (≠ E143)
Sites not aligning to the query:
4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
30% identity, 96% coverage: 1:321/336 of query aligns to 4:320/337 of 4ilkA
- active site: C40 (= C37), G41 (= G38), S42 (= S39), H45 (= H42), H59 (= H58), E60 (= E59), C89 (= C88), C92 (= C91), C95 (= C94), C103 (= C102), A107 (≠ T106), P145 (= P144), A149 (≠ C148)
- binding manganese (ii) ion: C40 (= C37), H59 (= H58), E60 (= E59), E144 (= E143)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G167), G170 (= G169), P171 (≠ V170), I172 (= I171), D193 (≠ T191), R194 (= R192), R198 (= R196), N213 (≠ P211), A235 (≠ C234), A236 (= A235), C237 (≠ G236), I241 (≠ T240), M258 (≠ F257), F260 (≠ V259)
- binding zinc ion: C89 (= C88), C92 (= C91), C95 (= C94), C103 (= C102)
Sites not aligning to the query:
3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
32% identity, 90% coverage: 16:319/336 of query aligns to 19:328/347 of 3gfbA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H65 (= H58), E66 (= E59), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102), K113 (≠ T106), P151 (= P144), A155 (= A151)
- binding nicotinamide-adenine-dinucleotide: G173 (= G167), G175 (= G169), P176 (≠ V170), L177 (≠ I171), S196 (≠ I190), E197 (≠ T191), P198 (≠ R192), R202 (= R196), F241 (≠ C234), S242 (≠ A235), A244 (≠ V237), L264 (≠ F257), G265 (= G258), L266 (≠ V259), I289 (≠ V278), T290 (≠ D279)
Sites not aligning to the query:
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
32% identity, 90% coverage: 16:319/336 of query aligns to 21:330/350 of Q5JI69
- L179 (≠ I171) binding NAD(+)
- E199 (≠ T191) binding NAD(+)
- R204 (= R196) binding NAD(+)
- LGL 266:268 (≠ FGV 257:259) binding NAD(+)
- IT 291:292 (≠ VD 278:279) binding NAD(+)
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
33% identity, 100% coverage: 1:336/336 of query aligns to 1:341/343 of 2ejvA
- active site: C38 (= C37), G39 (= G38), T40 (≠ S39), H43 (= H42), H63 (= H58), E64 (= E59), C93 (= C88), C96 (= C91), C99 (= C94), C107 (= C102), Q111 (≠ T106), P149 (= P144), A153 (≠ C148), K336 (= K331)
- binding nicotinamide-adenine-dinucleotide: G172 (= G167), G174 (= G169), P175 (≠ V170), I176 (= I171), S195 (≠ T191), D196 (≠ R192), P197 (≠ Q193), R201 (= R197), F238 (≠ C234), S239 (≠ A235), N241 (≠ V237), A244 (≠ T240), L261 (≠ F257), G262 (= G258), I263 (≠ V259)
- binding zinc ion: C38 (= C37), H63 (= H58), E64 (= E59), C96 (= C91), C99 (= C94), C107 (= C102)
2dq4A Crystal structure of threonine 3-dehydrogenase
33% identity, 100% coverage: 1:336/336 of query aligns to 1:341/343 of 2dq4A
- active site: C38 (= C37), G39 (= G38), T40 (≠ S39), H43 (= H42), H63 (= H58), E64 (= E59), C93 (= C88), C96 (= C91), C99 (= C94), C107 (= C102), Q111 (≠ T106), P149 (= P144), A153 (≠ C148), K336 (= K331)
- binding zinc ion: C38 (= C37), H63 (= H58), E64 (= E59), C93 (= C88), C96 (= C91), C107 (= C102)
Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
33% identity, 100% coverage: 1:336/336 of query aligns to 1:341/343 of Q5SKS4
- C38 (= C37) binding Zn(2+)
- H63 (= H58) binding Zn(2+)
- E64 (= E59) binding Zn(2+)
- C93 (= C88) binding Zn(2+)
- C96 (= C91) binding Zn(2+)
- C99 (= C94) binding Zn(2+)
- C107 (= C102) binding Zn(2+)
- I176 (= I171) binding NAD(+)
- D196 (≠ R192) binding NAD(+)
- R201 (= R197) binding NAD(+)
- LGI 261:263 (≠ FGV 257:259) binding NAD(+)
- IAG 286:288 (vs. gap) binding NAD(+)
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
32% identity, 88% coverage: 16:309/336 of query aligns to 19:317/346 of 2dfvA
- active site: C40 (= C37), G41 (= G38), T42 (≠ S39), H45 (= H42), H65 (= H58), E66 (= E59), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102), K113 (≠ T106), P151 (= P144), A155 (≠ C148)
- binding nicotinamide-adenine-dinucleotide: G175 (= G169), P176 (≠ V170), L177 (≠ I171), E197 (≠ T191), P198 (≠ R192), R202 (= R196), F241 (≠ C234), S242 (≠ A235), A244 (≠ V237), L264 (≠ F257), G265 (= G258), L266 (≠ V259), I289 (≠ V278), T290 (≠ D279)
- binding zinc ion: C95 (= C88), C101 (= C94), C109 (= C102)
Sites not aligning to the query:
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
32% identity, 88% coverage: 16:309/336 of query aligns to 21:319/348 of O58389
- C42 (= C37) binding Zn(2+)
- T44 (≠ S39) mutation to A: Total loss of enzymatic activity.
- H67 (= H58) binding Zn(2+)
- E68 (= E59) binding Zn(2+)
- C97 (= C88) binding Zn(2+)
- C100 (= C91) binding Zn(2+)
- C103 (= C94) binding Zn(2+)
- C111 (= C102) binding Zn(2+)
- E152 (= E143) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I171) binding NAD(+)
- E199 (≠ T191) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- R204 (= R196) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (≠ FGV 257:259) binding NAD(+)
- IT 291:292 (≠ VD 278:279) binding NAD(+)
- R294 (= R281) mutation to A: 4000-fold decrease in catalytic efficiency.
2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
29% identity, 96% coverage: 1:323/336 of query aligns to 1:333/347 of 2eerB
- active site: C38 (= C37), H39 (≠ G38), S40 (= S39), H43 (= H42), H68 (= H58), E69 (= E59), E98 (≠ C88), C101 (= C91), C104 (= C94), C112 (= C102), R116 (≠ T106), C154 (= C147), T158 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: C38 (= C37), H39 (≠ G38), S40 (= S39), H43 (= H42), C154 (= C147), T158 (vs. gap), G178 (= G167), G181 (= G169), G182 (≠ V170), L183 (≠ I171), D203 (≠ T191), V204 (≠ R192), R205 (≠ Q193), L247 (≠ C234), N248 (≠ A235), V270 (≠ F257), G271 (= G258), L272 (≠ V259), F273 (≠ T260), L295 (≠ F288), V296 (≠ T289)
- binding zinc ion: E98 (≠ C88), C101 (= C91), C104 (= C94), C112 (= C102)
Sites not aligning to the query:
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
31% identity, 91% coverage: 14:319/336 of query aligns to 13:327/348 of 1e3jA
- active site: C38 (= C37), G39 (= G38), S40 (= S39), H43 (= H42), H63 (= H58), E64 (= E59), C93 (= C88), C96 (= C91), C99 (= C94), C107 (= C102), T111 (= T106), P150 (= P144), G154 (≠ C148)
- binding phosphate ion: A174 (≠ G168), A196 (≠ T191), R197 (= R192), S198 (≠ Q193), R201 (= R196)
- binding zinc ion: C38 (= C37), H63 (= H58), E64 (= E59), C93 (= C88), C96 (= C91), C99 (= C94), C107 (= C102)
Sites not aligning to the query:
5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
31% identity, 91% coverage: 12:316/336 of query aligns to 12:318/339 of 5kiaA
- active site: C37 (= C37), G38 (= G38), T39 (≠ S39), H42 (= H42), H61 (= H58), E62 (= E59), C91 (= C88), C94 (= C91), C97 (= C94), C105 (= C102), V109 (≠ T106), P147 (= P144), A151 (≠ C148)
- binding calcium ion: D146 (≠ E143), N150 (≠ C147), E288 (≠ P287)
- binding zinc ion: C91 (= C88), C94 (= C91), C97 (= C94), C105 (= C102)
Sites not aligning to the query:
4oh1A Crystal structure of a putative zinc-binding dehydrogenase (gutb) from clostridium scindens atcc 35704 at 2.00 a resolution
30% identity, 81% coverage: 1:273/336 of query aligns to 10:284/351 of 4oh1A
- active site: C46 (= C37), G47 (= G38), S48 (= S39), H51 (= H42), H74 (= H58), E75 (= E59), C106 (= C88), C109 (= C91), C112 (= C94), C120 (= C102), P158 (= P144), A162 (≠ C148)
- binding zinc ion: C46 (= C37), H74 (= H58), E75 (= E59), C106 (= C88), C109 (= C91), C112 (= C94), C120 (= C102), E157 (= E143)
Sites not aligning to the query:
6ie0B X-ray crystal structure of 2r,3r-butanediol dehydrogenase from bacillus subtilis
31% identity, 100% coverage: 1:336/336 of query aligns to 2:346/347 of 6ie0B
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
28% identity, 94% coverage: 1:316/336 of query aligns to 1:320/341 of P07913
- C38 (= C37) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
28% identity, 97% coverage: 10:335/336 of query aligns to 18:349/357 of P27867
- C45 (= C37) binding Zn(2+)
- H70 (= H58) binding Zn(2+)
- E71 (= E59) binding Zn(2+)
- E156 (= E143) binding Zn(2+)
- D204 (≠ T191) binding NAD(+)
- R209 (= R196) binding NAD(+)
- VGM 273:275 (≠ FGV 257:259) binding NAD(+)
- VFR 297:299 (≠ AYL 283:285) binding NAD(+)
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
30% identity, 91% coverage: 10:316/336 of query aligns to 18:330/357 of Q00796
- C45 (= C37) binding Zn(2+)
- H70 (= H58) binding Zn(2+)
- E71 (= E59) binding Zn(2+)
- R110 (= R98) to P: in HMNR8; results in protein aggregation
- H135 (≠ V122) to R: in HMNR8; results in protein aggregation
- A153 (≠ V140) to D: in HMNR8; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I171) binding NAD(+)
- D204 (≠ T191) binding NAD(+)
- R209 (= R196) binding NAD(+)
- Q239 (≠ G226) to L: in dbSNP:rs1042079
- N269 (≠ S253) to T: in dbSNP:rs930337
- VGL 273:275 (≠ FGV 257:259) binding NAD(+)
- VFR 297:299 (≠ AYL 283:285) binding NAD(+)
- V322 (= V308) to I: in HMNR8; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Query Sequence
>WP_011650428.1 NCBI__GCF_000009265.1:WP_011650428.1
MRAVRLESIGSLTMRSVEKPVAGPGELLVRVSVAGICGSDRHMYKGEYPTAIPVTLGHEF
CGIVEAIGDTVTRFTGGELVTVDPNIACGTCRACTQARPNLCESLTAIGVTRDGGFAEYV
AVPQAQAFVLPADLDPVHGVFSEPLACCIHAIDKARIRPGDSVAILGGGVIGLLMVQLAR
LAGAGEVVLITRQQSRRQTALRLGATHAFDPAASDTIASVREVTKGGADVVIECAGVSDT
LQSGLKMARRGGSFVLFGVTPAGVEVPVLPFDLLVNEVDIRPAYLNPFTHSRAAAMVASG
ALELDALVTKTIGLEEVADVVGNAPLPGEIKVIVRP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory