SitesBLAST
Comparing WP_011735248.1 NCBI__GCF_000015045.1:WP_011735248.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
50% identity, 92% coverage: 25:353/357 of query aligns to 26:359/363 of 3dtgA
- active site: K199 (= K197)
- binding O-benzylhydroxylamine: F72 (= F71), Y171 (= Y169), Y204 (= Y202)
- binding pyridoxal-5'-phosphate: R96 (= R95), R189 (= R187), K199 (= K197), Y204 (= Y202), E235 (= E233), G238 (≠ S236), L263 (= L257), I266 (= I260), T267 (= T261), T309 (= T303)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
50% identity, 92% coverage: 25:353/357 of query aligns to 26:359/363 of 3dtfA
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
50% identity, 92% coverage: 25:353/357 of query aligns to 31:364/368 of A0R066
- R101 (= R95) binding pyridoxal 5'-phosphate
- Y209 (= Y202) binding pyridoxal 5'-phosphate
- K299 (≠ D288) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T303) binding pyridoxal 5'-phosphate
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
49% identity, 91% coverage: 30:353/357 of query aligns to 3:331/335 of 3ht5A
- active site: K171 (= K197)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R95), K171 (= K197), Y176 (= Y202), E207 (= E233), G210 (≠ S236), M211 (= M237), N212 (= N238), L235 (= L257), G237 (= G259), I238 (= I260), T239 (= T261), T281 (= T303)
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
47% identity, 91% coverage: 30:353/357 of query aligns to 2:322/326 of 5u3fA
- active site: K162 (= K197)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G73), R67 (= R95), Y110 (= Y137), K162 (= K197), Y167 (= Y202), E198 (= E233), G201 (≠ S236), M202 (= M237), N203 (= N238), L226 (= L257), G228 (= G259), I229 (= I260), T230 (= T261), T272 (= T303)
7nwmA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 12 (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 13:355/365 of 7nwmA
- binding pyridoxal-5'-phosphate: R98 (= R95), R191 (= R187), K201 (= K197), Y206 (= Y202), E236 (= E233), T239 (≠ S236), M240 (= M237), L265 (= L257), G267 (= G259), V268 (≠ I260), T269 (= T261), G311 (= G302), T312 (= T303)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methoxy-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F25), Y69 (= Y66), F74 (= F71), Y140 (= Y137), G153 (= G150), V154 (= V151), Y172 (= Y169), Q213 (≠ A209), Q223 (= Q219), T239 (≠ S236), A313 (= A304)
7nweA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 10 (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 13:355/365 of 7nweA
- binding pyridoxal-5'-phosphate: R98 (= R95), K201 (= K197), Y206 (= Y202), E236 (= E233), T239 (≠ S236), M240 (= M237), L265 (= L257), G267 (= G259), V268 (≠ I260), T269 (= T261), G311 (= G302), T312 (= T303)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F25), Y69 (= Y66), F74 (= F71), Y140 (= Y137), G153 (= G150), V154 (= V151), Y172 (= Y169), Q213 (≠ A209), Q223 (= Q219), T239 (≠ S236), A313 (= A304)
7nwcA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 2 (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 13:355/365 of 7nwcA
- binding pyridoxal-5'-phosphate: R98 (= R95), R191 (= R187), K201 (= K197), Y206 (= Y202), E236 (= E233), T239 (≠ S236), M240 (= M237), N241 (= N238), L265 (= L257), G267 (= G259), V268 (≠ I260), T269 (= T261), G311 (= G302), T312 (= T303)
- binding 3-(5-chloranyl-2,4-dimethoxy-phenyl)-6-(trifluoromethyl)-1H-pyrimidine-2,4-dione: F28 (= F25), Y172 (= Y169), Q223 (= Q219), T239 (≠ S236), A313 (= A304)
7nwbA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 1 (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 13:355/365 of 7nwbA
- binding pyridoxal-5'-phosphate: R98 (= R95), R191 (= R187), K201 (= K197), Y206 (= Y202), E236 (= E233), T239 (≠ S236), M240 (= M237), L265 (= L257), G267 (= G259), V268 (≠ I260), T269 (= T261), G311 (= G302), T312 (= T303)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F25), Y69 (= Y66), F74 (= F71), Y140 (= Y137), G153 (= G150), V154 (= V151), Y172 (= Y169), Q213 (≠ A209), Q223 (= Q219), A313 (= A304)
7nwaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and compound a (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 13:355/365 of 7nwaA
- binding pyridoxal-5'-phosphate: R98 (= R95), K201 (= K197), Y206 (= Y202), E236 (= E233), T239 (≠ S236), N241 (= N238), L265 (= L257), G267 (= G259), V268 (≠ I260), T269 (= T261), T312 (= T303)
- binding 2-[[4-chloranyl-2,6-bis(fluoranyl)phenyl]methylamino]-7-oxidanylidene-5-propyl-4H-pyrazolo[1,5-a]pyrimidine-3-carbonitrile: R142 (= R139), G153 (= G150), V154 (= V151), Y172 (= Y169), F173 (≠ Y170), V181 (= V177), Q223 (= Q219), V237 (= V234), T239 (≠ S236), M240 (= M237), G311 (= G302), A313 (= A304), C317 (≠ S308)
7nyaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor bay-069 (compound 36) (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 12:354/364 of 7nyaA
- binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R187), K200 (= K197), Y205 (= Y202), E235 (= E233), T238 (≠ S236), M239 (= M237), L264 (= L257), G266 (= G259), V267 (≠ I260), T268 (= T261), G310 (= G302), T311 (= T303)
- binding 3-[4-chloranyl-3-(2-methylphenoxy)naphthalen-1-yl]-6-(trifluoromethyl)-5~{H}-pyrimidine-2,4-dione: F27 (= F25), Y68 (= Y66), F73 (= F71), Y139 (= Y137), G152 (= G150), V153 (= V151), Y171 (= Y169), Q212 (≠ A209), Q222 (= Q219), T238 (≠ S236), A312 (= A304)
7ny9A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 38 (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 12:354/364 of 7ny9A
- binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R187), K200 (= K197), Y205 (= Y202), E235 (= E233), T238 (≠ S236), M239 (= M237), N240 (= N238), L264 (= L257), G266 (= G259), V267 (≠ I260), T268 (= T261), T311 (= T303)
- binding 3-[2,6-bis(oxidanylidene)-4-(trifluoromethyl)-5~{H}-pyrimidin-1-yl]-~{N}-methyl-2-pyridin-2-yl-imidazo[1,2-a]pyridine-7-carboxamide: F27 (= F25), Y139 (= Y137), G152 (= G150), V153 (= V151), Y171 (= Y169), Q212 (≠ A209), Q221 (≠ D218), Q222 (= Q219), V223 (= V220), T238 (≠ S236)
7ny2A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 35 (see paper)
43% identity, 94% coverage: 9:345/357 of query aligns to 12:354/364 of 7ny2A
- binding pyridoxal-5'-phosphate: R97 (= R95), K200 (= K197), Y205 (= Y202), E235 (= E233), T238 (≠ S236), M239 (= M237), L264 (= L257), G266 (= G259), V267 (≠ I260), T268 (= T261), T311 (= T303)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-2-(2-methylphenoxy)naphthalene-1-carbonitrile: F27 (= F25), Y139 (= Y137), G152 (= G150), V153 (= V151), Y171 (= Y169), Q212 (≠ A209), Q222 (= Q219), A312 (= A304)
2abjA Crystal structure of human branched chain amino acid transaminase in a complex with an inhibitor, c16h10n2o4f3scl, and pyridoxal 5' phosphate. (see paper)
42% identity, 97% coverage: 9:353/357 of query aligns to 14:362/366 of 2abjA
- active site: K202 (= K197)
- binding n'-(5-chlorobenzofuran-2-carbonyl)-2-(trifluoromethyl)benzenesulfonohydrazide: F29 (= F25), F75 (= F71), Y141 (= Y137), Y173 (= Y169), K202 (= K197), Q224 (= Q219), T240 (≠ S236), M241 (= M237), G312 (= G302), A314 (= A304), C315 (≠ A305)
- binding pyridoxal-5'-phosphate: R99 (= R95), R192 (= R187), K202 (= K197), Y207 (= Y202), E237 (= E233), T240 (≠ S236), M241 (= M237), N242 (= N238), L266 (= L257), G268 (= G259), V269 (≠ I260), T270 (= T261), T313 (= T303)
7nxoA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 24(5-f) (see paper)
42% identity, 94% coverage: 9:345/357 of query aligns to 12:351/361 of 7nxoA
- binding pyridoxal-5'-phosphate: R97 (= R95), K197 (= K197), Y202 (= Y202), E232 (= E233), T235 (≠ S236), M236 (= M237), N237 (= N238), L261 (= L257), G263 (= G259), V264 (≠ I260), T265 (= T261), T308 (= T303)
- binding 4-[2,4-bis(oxidanylidene)-6-(phenylsulfonyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F27 (= F25), Y68 (= Y66), F73 (= F71), G152 (= G150), V153 (= V151), Y171 (= Y169), F172 (≠ Y170), Q209 (≠ A209), Q218 (≠ D218), Q219 (= Q219), T235 (≠ S236)
7nxnA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 21(5-f) (see paper)
42% identity, 94% coverage: 9:345/357 of query aligns to 13:352/362 of 7nxnA
- binding pyridoxal-5'-phosphate: R98 (= R95), R188 (= R187), K198 (= K197), Y203 (= Y202), E233 (= E233), T236 (≠ S236), M237 (= M237), N238 (= N238), L262 (= L257), G264 (= G259), V265 (≠ I260), T266 (= T261), T309 (= T303)
- binding 4-[6-[bis(fluoranyl)-phenyl-methyl]-2,4-bis(oxidanylidene)-1~{H}-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F25), Y69 (= Y66), F74 (= F71), G153 (= G150), V154 (= V151), Y172 (= Y169), Q210 (≠ A209), Q219 (≠ D218), Q220 (= Q219), T236 (≠ S236)
7ntrA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and substrate mimic 3-phenylpropionate (see paper)
42% identity, 94% coverage: 9:345/357 of query aligns to 13:352/362 of 7ntrA
- binding hydrocinnamic acid: F28 (= F25), Y69 (= Y66), V154 (= V151), T236 (≠ S236), G308 (= G302), T309 (= T303), A310 (= A304)
- binding pyridoxal-5'-phosphate: R98 (= R95), R188 (= R187), K198 (= K197), Y203 (= Y202), E233 (= E233), T236 (≠ S236), M237 (= M237), L262 (= L257), G264 (= G259), V265 (≠ I260), T266 (= T261), G308 (= G302), T309 (= T303)
5i60A X-ray crystal structure at 2.12a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biarl amide compound and an internal aldimine linked plp cofactor. (see paper)
42% identity, 87% coverage: 12:322/357 of query aligns to 11:326/359 of 5i60A
- active site: K196 (= K197)
- binding (2-{[4-(1H-pyrazol-3-yl)phenyl]carbamoyl}phenyl)acetic acid: F24 (= F25), Y135 (= Y137), G165 (= G167), Y167 (= Y169), T234 (≠ S236), G306 (= G302), A308 (= A304)
- binding pyridoxal-5'-phosphate: R93 (= R95), R186 (= R187), K196 (= K197), Y201 (= Y202), E231 (= E233), T234 (≠ S236), M235 (= M237), L260 (= L257), G262 (= G259), V263 (≠ I260), V264 (≠ T261), T307 (= T303)
5hneA X-ray crystal structure of human mitochondrial branched chain aminotransferase (bcatm) complexed with a 2-aryl benzimidazole compound and an internal aldimine linked plp cofactor (see paper)
42% identity, 87% coverage: 12:322/357 of query aligns to 11:326/359 of 5hneA
- active site: K196 (= K197)
- binding 1-[(1R,3S)-3-{[(5-bromothiophen-2-yl)carbonyl]amino}cyclohexyl]-N-methyl-2-(pyridin-2-yl)-1H-benzimidazole-5-carboxamide: F24 (= F25), F69 (= F71), K73 (= K75), Y167 (= Y169), Q218 (= Q219), V232 (= V234), G233 (= G235), T234 (≠ S236), M235 (= M237), A308 (= A304), C312 (≠ S308)
- binding pyridoxal-5'-phosphate: R93 (= R95), K196 (= K197), Y201 (= Y202), E231 (= E233), T234 (≠ S236), N236 (= N238), L260 (= L257), G262 (= G259), V263 (≠ I260), V264 (≠ T261), G306 (= G302), T307 (= T303)
2coiA Crystal structure of oxidized human cytosolic branched-chain aminotransferase complexed with gabapentin (see paper)
42% identity, 94% coverage: 9:345/357 of query aligns to 13:349/358 of 2coiA
- active site: K195 (= K197)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: Y68 (= Y66), R141 (= R139), V153 (= V151), T233 (≠ S236), G305 (= G302), T306 (= T303), A307 (= A304)
- binding pyridoxal-5'-phosphate: R97 (= R95), R185 (= R187), K195 (= K197), Y200 (= Y202), E230 (= E233), T233 (≠ S236), L259 (= L257), G261 (= G259), V262 (≠ I260), T263 (= T261), T306 (= T303)
Query Sequence
>WP_011735248.1 NCBI__GCF_000015045.1:WP_011735248.1
MDIQVLPLPAEKMKQKAQDETKLSFGRQFTDRMLLVEWKADQGWCDARIQPYAPFVLDPS
CLVFHYAQEIFEGLKAYKWADGRIALFRPEMNARRFNQSADRLCMPDVPEELFLGGIEKL
LSLEKEWIPGAPGTSLYIRPAMIAVEPVLGVKPSSHYYFFVILSPVGAYYSAGFNPVSIM
VEDHYVRAVPGGTGEAKTGGNYASSLKGALEAKNKGYDQVLWLDGREQRYVEEVGSMNMF
FVYGDTIVTAAMNGSILSGITRDSILKLAVSLGYRAEERSIEINELMDDIRGGKVTEAFG
SGTAAVVSPVGKLCFKDEVVHLSGGTVGAITQKLYDTLTGIQTGKIKDEFGWVRFVA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory