SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011735584.1 NCBI__GCF_000015045.1:WP_011735584.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
53% identity, 99% coverage: 1:396/399 of query aligns to 3:396/398 of 1vpeA

query
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1vpeA

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active site: R35 (= R38), K196 (= K200), G353 (= G353), G376 (= G376)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G198), A195 (= A199), K196 (= K200), K200 (= K204), G218 (= G222), A219 (≠ G223), N316 (= N319), P318 (= P321), G320 (= G323), V321 (= V324), E323 (= E326), G352 (= G352), G353 (= G353), D354 (= D354), S355 (≠ T355)

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
54% identity, 97% coverage: 11:396/399 of query aligns to 10:394/394 of P40924

query
sites
P40924

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S183 (≠ Q186) modified: Phosphoserine
T299 (≠ S302) modified: Phosphothreonine

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
53% identity, 97% coverage: 11:398/399 of query aligns to 10:399/654 of P36204

query
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P36204

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R36 (= R38) binding substrate
R118 (= R120) binding substrate
R151 (= R153) binding substrate

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
55% identity, 97% coverage: 11:396/399 of query aligns to 10:394/394 of 1phpA

query
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1phpA

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P

A

A

V

A

A

K

K

R

R

L

L

S

G

E

E

L

L

L

L

G

E

R

R

K

P

V

V

K

A

Q

K

A

T

S

N

D

E

C

A

F

V

G

G

E

D

E

E

V

V

T

K

R

A

E

A

I

V

D

D

A

R

M

L

K

N

S

E

G

G

E

D

V

V

L

L

M

L

L

L

E

E

N

N

V

V

R

R

F

F

Y

Y

P

P

G

G

E

E

E

E

K

K

N

N

D

D

P

P

K

E

F

L

A

A

Q

K

Q

A

L

F

A

A

N

E

G

L

C

A

Q

D

V

L

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

A

S

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

E

E

A

G

I

I

T

A

R

H

V

Y

I

L

P

P

T

A

V

V

V

-

A

A

G

G

F

F

L

L

M

M

R

E

N

K

E

E

M

L

T

E

F

V

F

L

D

G

K

K

A

A

M

L

Q

S

N

N

P

P

V

D

R

R

P

P

L

F

V

T

A

A

I

I

L

I

G

G

G
|
G

A

A

K
|
K

V

V

S

K

G

D

K
|
K

L

I

E

G

V

V

L

I

E

D

K

N

L

L

M

L

N

E

K

K

V

V

D

D

K

N

V

L

V

I

I

I

G

G

G
|
G

G
|
G

M

L

A

A

F

Y

T

T

F

F

L

V

K

K

S

A

M

L

G

G

Y

H

S

D

V

V

G

G

A

K

S

S

K
x
L

V

L

E

E

D

E

E

D

L

K

L

I

P

E

T

L

A

A

K

K

K

S

I

F

M

M

D

E

K

K

A

A

R

K

K

E

K

K

G

G

I

V

M

R

F

F

Y

Y

L

M

P

P

V

V

D

D

C

V

V

V

V

V

A

A

N

D

A

R

F

F

E

A

A

N

S

D

A

A

T

N

N

T

F

K

I

V

T

V

T

P

V

I

Q

D

E

A

I

I

P

P

E

A

G

D

W

W

L

S

A

A

L

L

D

D

I

I

G

G

P

P

A

K

S

T

A

R

T

E

L

L

F

Y

T

R

E

D

T

V

L

I

R

R

D

E

A

S

K

K

T

L

V

V

I

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

D

D

A

A

F

F

A

A

R

H

G

G

T

T

F

K

A

A

V

I

A

A

E

E

A

A

V

L

A

A

S

E

A

A

F

L

A

D

T

T

-

Y

T

S

I

V

I

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

D

A

S

A

A

A

V

V

R

E

K

K

A

F

G

G

V

L

E

A

S

D

K

K

V

M

S

D

Y

H

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

E

E

L

F

L

M

E

E

G

G

K

K

V

Q

L

L

P

P

G

G

V

V

K

V

A

A

L

L

D

E

I

D

K

K

active site: R36 (= R38), K197 (= K200), G351 (= G353), G374 (= G376)
binding adenosine-5'-diphosphate: G195 (= G198), K201 (= K204), G219 (= G222), G220 (= G223), L237 (≠ K240), N316 (= N319), P318 (= P321), G320 (= G323), V321 (= V324), E323 (= E326), G350 (= G352), D352 (= D354), S353 (≠ T355)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
55% identity, 97% coverage: 11:396/399 of query aligns to 10:394/394 of P18912

query
sites
P18912

D

D

L

V

K

R

D

G

K

K

K

R

V

V

F

F

I

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

M

D

E

S

-

K

Q

G

G

N

A

I

I

T

T

E

D

D

D

T

T

R

R

I

I

T

R

G

A

A

A

L

L

P

P

T

T

I

I

R

R

Y

Y

A

L

I

I

E

E

Q

H

G

G

A

A

R

K

V

V

I

I

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

E

K

K

V

N

V

E

E

K

E

Y

L

S

R

M

L

V

D

P

A

A

V

A

A

K

K

R

R

L

L

S

G

E

E

L

L

L

L

G

E

R

R

K

P

V

V

K

A

Q

K

A

T

S

N

D

E

C

A

F

V

G

G

E

D

E

E

V

V

T

K

R

A

E

A

I

V

D

D

A

R

M

L

K

N

S

E

G

G

E

D

V

V

L

L

M

L

L

L

E

E

N

N

V

V

R

R

F

F

Y

Y

P

P

G

G

E

E

E

E

K

K

N

N

D

D

P

P

K

E

F

L

A

A

Q

K

Q

A

L

F

A

A

N

E

G

L

C

A

Q

D

V

L

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

A

S

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

E

E

A

G

I

I

T

A

R

H

V

Y

I

L

P

P

T

A

V

V

V

-

A

A

G

G

F

F

L

L

M

M

R

E

N

K

E

E

M

L

T

E

F

V

F

L

D

G

K

K

A

A

M

L

Q

S

N

N

P

P

V

D

R

R

P

P

L

F

V

T

A

A

I

I

L

I

G

G

G

G

A

A

K

K

V

V

S

K

G

D

K
|
K

L

I

E

G

V

V

L

I

E

D

K

N

L

L

M

L

N

E

K

K

V

V

D

D

K

N

V

L

V

I

I

I

G

G

G

G

G

G

M

L

A

A

F

Y

T

T

F

F

L

V

K

K

S

A

M

L

G

G

Y

H

S

D

V

V

G

G

A

K

S

S

K

L

V

L

E

E

D

E

E

D

L

K

L

I

P

E

T

L

A

A

K

K

K

S

I

F

M

M

D

E

K

K

A

A

R

K

K

E

K

K

G

G

I

V

M

R

F

F

Y

Y

L

M

P

P

V

V

D

D

C

V

V

V

V

V

A

A

N

D

A

R

F

F

E

A

A

N

S

D

A

A

T

N

N

T

F

K

I

V

T

V

T

P

V

I

Q

D

E

A

I

I

P

P

E

A

G

D

W

W

L

S

A

A

L

L

D

D

I

I

G

G

P

P

A

K

S

T

A

R

T

E

L

L

F

Y

T

R

E

D

T

V

L

I

R

R

D

E

A

S

K

K

T

L

V

V

I

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

M

M

D

D

A

A

F

F

A

A

R

H

G

G

T

T

F

K

A

A

V

I

A

A

E

E

A

A

V

L

A

A

S

E

A

A

F

L

A

D

T

T

-

Y

T

S

I

V

I

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

D

A

S

A

A

A

V

V

R

E

K

K

A

F

G

G

V

L

E

A

S

D

K

K

V

M

S

D

Y

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

E

E

L

F

L

M

E

E

G

G

K

K

V

Q

L

L

P

P

G

G

V

V

K

V

A

A

L

L

D

E

I

D

K

K

K201 (= K204) binding ATP
N316 (= N319) binding ATP
E323 (= E326) binding ATP
GGDS 350:353 (≠ GGDT 352:355) binding ATP

3pgkA The structure of yeast phosphoglycerate kinase at 0.25 nm resolution (see paper)
49% identity, 97% coverage: 8:396/399 of query aligns to 9:414/415 of 3pgkA

query
sites
3pgkA

Q

Q

L

D

A

L

D

D

L

L

K

K

D

D

K

K

K

R

V

V

F

F

I

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

L

D

D

S

G

K

K

G

-

N

K

I

I

T

T

E

S

D

N

T

Q

R
|
R

I

I

T

V

G

A

A

A

L

L

P

P

T

T

I

I

R

K

Y

Y

A

V

I

L

E

E

Q

H

G

H

A

P

R

R

-

Y

V

V

I

V

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

E

E

K

R

N

N

E

E

K

K

Y

Y

S

S

M

L

V

A

P

P

A

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

L

L

G

G

R

K

K

D

V

V

K

T

Q

F

A

L

S

N

D

D

C

C

F

V

G

G

E

P

E

E

V

V

T

E

R

A

E

A

I

V

D

K

A

A

M

S

K

A

S

P

G

G

E

S

V

V

L

I

M

L

L

L

E

E

N

N

V

L

R

R

F

Y

Y

H

P

I

G

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

K

K

N

E

D

D

-

V

P

Q

K

K

F

F

A

R

Q

H

Q

E

L

L

A

S

N

S

G

L

C

A

Q

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

F

T

D

R

-

V

-

I

L

P

P

T

Q

V

R

V

A

A

A

G

G

F

F

L

L

M

L

R

E

N

K

E

E

M

L

T

K

F

Y

F

F

D

G

K

K

A

A

M

L

Q

E

N

N

P

P

V

T

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

K

N

L

L

M

L

N

D

K

K

V

V

D

D

K

S

V

I

V

I

I

I

G

G

G

G

G

G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

S

V

M

L

-

E

G

N

Y

T

S

E

V

I

G

G

A

D

S

S

K

I

V

F

E

D

D

K

E

A

L

V

L

G

P

P

T

E

A

I

K

A

K

K

I

L

M

M

D

E

K

K

A

A

R

K

K

A

K

K

G

G

I

V

M

E

F

V

Y

V

L

L

P

P

V

V

D

D

C

F

V

I

V

I

A

A

N

D

A

A

F
|
F

E

S

A

A

S

S

A

A

-

N

T

T

N

K

F

T

I

V

T

T

T

D

V

K

Q

E

E

G

I

I

P

P

E

A

G

G

W

W

L

Q

A

G

L
|
L

D

D

I

N

G

G

P

P

A

E

S

S

A

R

T

K

L

L

F

F

T

A

E

A

T

T

L

V

R

A

D

K

A

A

K

T

T

V

V

I

I

L

W

W

N
|
N

G
|
G

P
|
P

M

P

G
|
G

V
|
V

F

F

E

E

M

F

D

E

A

K

F

F

A

A

R

A

G

G

T

T

F

K

A

A

V

L

A

L

E

D

A

E

V

V

A

V

S

K

A

S

F

S

A

A

-

A

-

G

-

N

T

T

T

V

I

I

I

I

G

G

G

G

G
|
G

D
|
D

T

T

D

A

S

T

A

V

V

A

R

K

K

K

A

Y

G

G

V

V

E

T

S

D

K

K

V

I

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

L

L

E

E

G

G

K

K

V

E

L

L

P

P

G

G

V

V

K

A

A

F

L

L

D

S

I

E

K

K

active site: R38 (= R38), K213 (= K200), G371 (= G353), G394 (= G376)
binding adenosine-5'-triphosphate: G211 (= G198), A212 (= A199), K213 (= K200), F289 (= F275), L311 (= L296), N334 (= N319), G335 (= G320), P336 (= P321), G338 (= G323), V339 (= V324), D372 (= D354)

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
50% identity, 97% coverage: 8:396/399 of query aligns to 10:415/416 of P00560

query
sites
P00560

Q

Q

L

D

A

L

D

D

L

L

K

K

D

D

K

K

K

R

V

V

F

F

I

I

R
|
R

V

V

D

D

F

F

N

N

V

V

P

P

Q

L

D

D

S

G

K

K

G

-

N

K

I

I

T

T

E

S

D

N

T

Q

R
|
R

I

I

T

V

G

A

A

A

L

L

P

P

T

T

I

I

R

K

Y

Y

A

V

I

L

E

E

Q

H

G

H

A

P

R

R

-

Y

V

V

I

V

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

E

E

K

R

N

N

E

E

K

K

Y

Y

S

S

M

L

V

A

P

P

A

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

L

L

G

G

R

K

K

D

V

V

K

T

Q

F

A

L

S

N

D

D

C

C

F

V

G

G

E

P

E

E

V

V

T

E

R

A

E

A

I

V

D

K

A

A

M

S

K

A

S

P

G

G

E

S

V

V

L

I

M

L

L

L

E

E

N

N

V

L

R
|
R

F

Y

Y

H

P

I

G

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

K

K

N

E

D

D

-

V

P

Q

K

K

F

F

A

R

Q

H

Q

E

L

L

A

S

N

S

G

L

C

A

Q

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R
|
R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

F

T

D

R

-

V

-

I

L

P

P

T

Q

V

R

V

A

A

A

G

G

F

F

L

L

M

L

R

E

N

K

E

E

M

L

T

K

F

Y

F

F

D

G

K

K

A

A

M

L

Q

E

N

N

P

P

V

T

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G

G

A

A

K

K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

K

N

L

L

M

L

N

D

K

K

V

V

D

D

K

S

V

I

V

I

I

I

G

G

G

G

G

G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

S

V

M

L

-

E

G

N

Y

T

S

E

V

I

G

G

A

D

S

S

-

I

-

F

-

D

K

K

V

A

E

G

D

A

E

E

L

I

L

V

P

P

T

-

A

-

K

-

K

K

I

L

M

M

D

E

K

K

A

A

R

K

K

A

K

K

G

G

I

V

M

E

F

V

Y

V

L

L

P

P

V

V

D

D

C

F

V

I

V

I

A

A

N

D

A

A

F

F

E

S

A

A

S

D

A

A

-

N

T

T

N

K

F

T

I

V

T

T

T

D

V

K

Q

E

E

G

I

I

P

P

E

A

G

G

W

W

L

Q

A

G

L

L

D

D

I

N

G

G

P

P

A

E

S

S

A

R

T

K

L

L

F

F

T

A

E

A

T

T

L

V

R

A

D

K

A

A

K

K

T

T

V

I

I

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

M

F

D

E

A

K

F

F

A

A

R

A

G

G

T

T

F

K

A

A

V

L

A

L

E

D

A

E

V

V

A

V

S

K

A

S

F

S

A

A

-

A

-

G

-

N

T

T

T

V

I

I

I

I

G

G

G

G

G

G

D

D

T

T

D

A

S

T

A

V

V

A

R

K

K

K

A

Y

G

G

V

V

E

T

S

D

K

K

V

I

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

E

E

L

L

L

L

E

E

G

G

K

K

V

E

L

L

P

P

G

G

V

V

K

A

A

F

L

L

D

S

I

E

K

K

R22 (= R20) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R38) binding substrate
R122 (= R120) binding substrate
R169 (= R153) binding substrate

1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
49% identity, 97% coverage: 8:396/399 of query aligns to 9:414/415 of 1qpgA

query
sites
1qpgA

Q

Q

L

D

A

L

D

D

L

L

K

K

D

D

K

K

K

R

V

V

F

F

I

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

L

D

D

S

G

K

K

G

-

N

K

I

I

T

T

E

S

D

N

T

Q

R
|
R

I

I

T

V

G

A

A

A

L

L

P

P

T

T

I

I

R

K

Y

Y

A

V

I

L

E

E

Q

H

G

H

A

P

R

R

-

Y

V

V

I

V

L

L

A

A

S

S

H

H

L

L

G

G

R

Q

P

P

K

N

G

G

E

E

K

R

N

N

E

E

K

K

Y

Y

S

S

M

L

V

A

P

P

A

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

L

L

G

G

R

K

K

D

V

V

K

T

Q

F

A

L

S

N

D

D

C

C

F

V

G

G

E

P

E

E

V

V

T

E

R

A

E

A

I

V

D

K

A

A

M

S

K

A

S

P

G

G

E

S

V

V

L

I

M

L

L

L

E

E

N

N

V

L

R

R

F

Y

Y

H

P

I

G

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

K

K

N

E

D

D

-

V

P

Q

K

K

F

F

A

R

Q

H

Q

E

L

L

A

S

N

S

G

L

C

A

Q

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

F

T

D

R

-

V

-

I

L

P

P

T

Q

V

R

V

A

A

A

G

G

F

F

L

L

M

L

R

E

N

K

E

E

M

L

T

K

F

Y

F

F

D

G

K

K

A

A

M

L

Q

E

N

N

P

P

V

T

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G

G

A

A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

K

N

L

L

M

L

N

D

K

K

V

V

D

D

K

S

V

I

V

I

I

I

G

G

G
|
G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

S

V

M

L

-

E

G

N

Y

T

S

E

V

I

G

G

A

D

S

S

-

I

-

F

-

D

K

K

V

A

E

G

D

A

E

E

L

I

L

V

P

P

T

-

A

-

K

-

K

K

I

L

M

M

D

E

K

K

A

A

R

K

K

A

K

K

G

G

I

V

M

E

F

V

Y

V

L

L

P

P

V

V

D

D

C

F

V

I

V

I

A

A

N

D

A

A

F

F

E

S

A

A

S

D

A

A

-

N

T

T

N

K

F

T

I

V

T

T

T

D

V

K

Q

E

E

G

I

I

P

P

E

A

G

G

W

W

L

Q

A

G

L

L

D

D

I

N

G

G

P

P

A

E

S

S

A

R

T

K

L

L

F

F

T

A

E

A

T

T

L

V

R

A

D

K

A

A

K

K

T

T

V

I

I

V

W

W

N
|
N

G

G

P
|
P

M

P

G
|
G

V
|
V

F
|
F

E
|
E

M

F

D

E

A

K

F

F

A

A

R

A

G

G

T

T

F

K

A

A

V

L

A

L

E

D

A

E

V

V

A

V

S

K

A

S

F

S

A

A

-

A

-

G

-

N

T

T

T

V

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

T
|
T

D

A

S

T

A

V

V

A

R

K

K

K

A

Y

G

G

V

V

E

T

S

D

K

K

V

I

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

L

L

E

E

G

G

K

K

V

E

L

L

P

P

G

G

V

V

K

A

A

F

L

L

D

S

I

E

K

K

active site: R38 (= R38), K213 (= K200), G371 (= G353), G394 (= G376)
binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G222), G236 (= G223), N334 (= N319), P336 (= P321), G338 (= G323), V339 (= V324), F340 (= F325), E341 (= E326), G370 (= G352), G371 (= G353), D372 (= D354), T373 (= T355)

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
47% identity, 97% coverage: 11:396/399 of query aligns to 13:414/414 of O60101

query
sites
O60101

D

D

L

L

K

K

D

G

K

K

K

N

V

V

F

L

I

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

L

D

D

S

G

K

D

G

-

N

R

I

I

T

T

E

N

D

N

T

A

R

R

I

I

T

V

G

G

A

A

L

L

P

P

T

T

I

I

R

K

Y

Y

A

A

I

L

E

E

Q

Q

G

Q

A

P

R

K

-

A

V

V

I

I

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

E

A

K

R

N

V

E

A

K

K

Y
|
Y

S
|
S

M

L

V

K

P

P

A

V

A

A

K

A

R

E

L

L

S

S

E

K

L

L

L

L

G

G

R

K

K

P

V

V

K

K

Q

F

A

L

S

D

D

D

C

C

F

V

G

G

E

P

E

E

V

V

T

E

R

K

E

A

I

C

D

K

A

E

M

A

K

K

S

G

G

G

E

E

V

V

L

I

M

L

L

L

E

E

N

N

V

L

R

R

F

F

Y

H

P

I

G

E

E

E

E

E

-

G

-

S

-

A

K

K

N

V

D

D

P

G

K

K

-

K

-

V

-

K

-

A

-

D

-

A

-
x
S

-

A

-

V

-

E

-

A

F

F

A

R

Q

K

Q

S

L

L

A

T

N

S

G

L

C

G

Q

D

V

I

Y

F

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A
x
S

S
|
S

V

M

E

V

A

G

I

V

T

D

R

-

V

-

I

L

P

P

T

R

V

V

V

-

A
x
S

G

G

F

F

L

L

M

M

R

K

N

K

E

E

M

L

T

D

F

Y

F

F

D

S

K

K

A

A

M

L

Q

E

N

N

P

P

V

A

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G

G

A

A

K

K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

K

N

L

L

M

L

N

D

K

K

V

V

D

N

K

R

V

L

V

I

I

I

G

C

G

G

G

G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

S

V

M

L

-

N

G

G

Y

M

S

K

V

I

G

G

A

D

S
|
S

K

L

V

F

E

D

D

E

E

A

L

G

L
x
S

P

K

T

N

A

V

K

E

K

S

I

M

M

M

D

A

K

K

A

A

R

K

K

K

K

N

G

N

I

V

M

E

F

V

Y

F

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

N

D

A

K

F

F

E

D

A

K

S

D

A

A

T

K

N

V

F

G

I

S

T

A

T
|
T

V

A

Q

E

E

E

-

G

I

I

P

P

E

D

G

G

W

W

L

M

A

G

L

L

D

D

I

C

G

G

P

P

A

K

S

S

A

S

T

A

L

K

F

F

T

A

E

E

T

V

L

I

R

T

D

T

A
x
S

K

K

T

T

V

I

I

V

W

W

N

N

G

G

P

P

M

A

G

G

V

V

F

F

E

E

M

F

D

D

A

N

F

F

A

A

R

K

G

G

T

T

F

K

A
x
S

V

M

A

L

E

D

A

A

V

C

-

V

-

K

-

T

A

C

S

E

A

A

F

G

A

N

T

V

T

V

I

I

I

V

G

G

G

G

G

G

D

D

T
|
T

D

A

S

T

A

V

V

A

R

K

K

K

A

Y

G

G

V

K

E

E

S

D

K

A

V

L

S
|
S

Y

H

I

V

S
|
S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

E

E

L

L

L

L

E

E

G

G

K

K

V

A

L

L

P

P

G

G

V

V

K

V

A

A

L

L

D
x
S

I
x
S

K

K

Y75 (= Y73) modified: Phosphotyrosine
S76 (= S74) modified: Phosphoserine
S143 (vs. gap) modified: Phosphoserine
S172 (≠ A156) modified: Phosphoserine
S173 (= S157) modified: Phosphoserine
S183 (≠ A170) modified: Phosphoserine
S253 (= S239) modified: Phosphoserine
S260 (≠ L246) modified: Phosphoserine
T299 (= T285) modified: Phosphothreonine
S328 (≠ A313) modified: Phosphoserine
S351 (≠ A336) modified: Phosphoserine
T373 (= T355) modified: Phosphothreonine
S387 (= S369) modified: Phosphoserine
S390 (= S372) modified: Phosphoserine
S412 (≠ D394) modified: Phosphoserine
S413 (≠ I395) modified: Phosphoserine

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
45% identity, 97% coverage: 10:396/399 of query aligns to 8:415/415 of 16pkA

query
sites
16pkA

A

C

D

D

L

L

K

K

D

G

K

K

K

K

V

V

F

L

I

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

V

D

K

S

N

K

-

G

G

N

K

I

I

T

T

E

N

D

D

T

Y

R
|
R

I

I

T

R

G

S

A

A

L

L

P

P

T

T

I

L

R

K

Y

K

A

V

I

L

E

T

Q

E

G

G

A

G

R

S

V

C

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

E

I

K

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

N

Q

E

Q

K

K

Y

A

S

T

M

L

V

K

P

P

A

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

L

L

G

L

R

R

K

P

V

V

K

T

Q

F

A

A

S

P

D

D

C

C

F

L

G

-

E

-

E

N

V

A

T

A

R

D

E

V

I

V

D

S

A

K

M

M

K

S

S

P

G

G

E

D

V

V

L

V

M

L

L

L

E

E

N

N

V

V

R

R

F

F

Y

Y

P

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

D

R

P

E

K

A

F

M

A

A

Q

K

Q

I

L

L

A

A

N

S

G

Y

C

G

Q

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

D

H

S

A

A

S

T

V

M

E

T

A

G

I

I

T

P

R

K

V

I

I

L

P

G

T

N

V

G

V

A

A

A

G

G

F

Y

L

L

M

M

R

E

N

K

E

E

M

I

T

S

F

Y

F

F

D

A

K

K

A

V

M

L

Q

G

N

N

P

P

V

P

R

R

P

P

L

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

G

D

K
|
K

L

I

E

Q

V

L

L

L

E

D

K

N

L

M

M

L

N

Q

K

R

V

I

D

D

K

Y

V

L

V

L

I

I

G

G

G

G

G
x
A

M

M

A

A

F
x
Y

T

T

F

F

L

L

K

K

S

A

M

Q

G

G

Y

Y

S

S

V

I

G

G

A

K

S

S

K

K

V

C

E

E

D

E

E

S

L

K

L

L

P

E

T

F

A

A

K

R

K

S

I

L

M

L

D

K

K

K

A

A

R

E

K

D

K

R

G

K

I

V

M

Q

F

V

Y

I

L

L

P

P

V

I

D

D

C

H

V

V

V

C

A

H

N

T

A

E

F

F

E

K

A

A

S

V

A

D

T

S

N

P

F

L

I

I

T

T

T

E

V

D

Q

Q

E

N

I

I

P

P

E

E

G

G

W

H

L

M

A

A

L
|
L

D

D

I

I

G

G

P

P

A

K

S

T

A

I

T

E

L

K

F

Y

T

V

E

Q

T

T

L

I

R

G

D

K

A

C

K

K

T

S

V

A

I

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

D

V

A

P

F

Y

A

S

R

K

G

G

T

T

F

F

A

A

V

I

A

A

E

K

A

A

V

M

A

G

S

R

A

G

F

T

A

H

-

E

-

H

-

G

-

L

T

M

T

S

I

I

I

I

G

G

G

G

G
|
G

D

D

T

S

D

A

S

S

A

A

V

A

R

E

K

L

A

S

G

G

V

E

E

A

S

K

K

R

V

M

S

S

Y

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

F

S

L

L

E

E

L

L

L

L

E

E

G

G

K

K

V

T

L

L

P

P

G

G

V

V

K

T

A

V

L

L

D

D

I

D

K

K

active site: R35 (= R38), K215 (= K200), G372 (= G353), G395 (= G376)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G198), A214 (= A199), K219 (= K204), A238 (≠ G223), Y241 (≠ F226), L311 (= L296), P336 (= P321), G338 (= G323), V339 (= V324), E341 (= E326), G393 (= G374), G394 (= G375), G395 (= G376)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
45% identity, 97% coverage: 10:396/399 of query aligns to 8:415/415 of 13pkA

query
sites
13pkA

A

C

D

D

L

L

K

K

D

G

K

K

K

K

V

V

F

L

I

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

V

D

K

S

N

K

-

G

G

N

K

I

I

T

T

E

N

D

D

T

Y

R
|
R

I

I

T

R

G

S

A

A

L

L

P

P

T

T

I

L

R

K

Y

K

A

V

I

L

E

T

Q

E

G

G

A

G

R

S

V

C

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

E

I

K

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

N

Q

E

Q

K

K

Y

A

S

T

M

L

V

K

P

P

A

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

L

L

G

L

R

R

K

P

V

V

K

T

Q

F

A

A

S

P

D

D

C

C

F

L

G

-

E

-

E

N

V

A

T

A

R

D

E

V

I

V

D

S

A

K

M

M

K

S

S

P

G

G

E

D

V

V

L

V

M

L

L

L

E

E

N

N

V

V

R

R

F

F

Y

Y

P

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

D

R

P

E

K

A

F

M

A

A

Q

K

Q

I

L

L

A

A

N

S

G

Y

C

G

Q

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

D

H

S

A

A

S

T

V

M

E

T

A

G

I

I

T

P

R

K

V

I

I

L

P

G

T

N

V

G

V

A

A

A

G

G

F

Y

L

L

M

M

R

E

N

K

E

E

M

I

T

S

F

Y

F

F

D

A

K

K

A

V

M

L

Q

G

N

N

P

P

V

P

R

R

P

P

L

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

G

D

K
|
K

L

I

E

Q

V

L

L

L

E

D

K

N

L

M

M

L

N

Q

K

R

V

I

D

D

K

Y

V

L

V

L

I

I

G

G

G

G

G

A

M

M

A

A

F

Y

T

T

F

F

L

L

K

K

S

A

M

Q

G

G

Y

Y

S

S

V

I

G

G

A

K

S

S

K

K

V

C

E

E

D

E

E

S

L

K

L

L

P

E

T

F

A

A

K

R

K

S

I

L

M

L

D

K

K

K

A

A

R

E

K

D

K

R

G

K

I

V

M

Q

F

V

Y

I

L

L

P

P

V

I

D

D

C

H

V

V

V

C

A

H

N

T

A

E

F

F

E

K

A

A

S

V

A

D

T

S

N

P

F

L

I

I

T

T

T

E

V

D

Q

Q

E

N

I

I

P

P

E

E

G

G

W

H

L

M

A

A

L
|
L

D

D

I

I

G

G

P

P

A

K

S

T

A

I

T

E

L

K

F

Y

T

V

E

Q

T

T

L

I

R

G

D

K

A

C

K

K

T

S

V

A

I

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

D

V

A

P

F

Y

A

S

R

K

G

G

T

T

F

F

A

A

V

I

A

A

E

K

A

A

V

M

A

G

S

R

A

G

F

T

A

H

-

E

-

H

-

G

-

L

T

M

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

D

A

S

S

A

A

V

A

R

E

K

L

A

S

G

G

V

E

E

A

S

K

K

R

V

M

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

L

L

E

E

G

G

K

K

V

T

L

L

P

P

G

G

V

V

K

T

A

V

L

L

D

D

I

D

K

K

active site: R35 (= R38), K215 (= K200), G372 (= G353), G395 (= G376)
binding adenosine-5'-diphosphate: G213 (= G198), A214 (= A199), K219 (= K204), L311 (= L296), P336 (= P321), G338 (= G323), V339 (= V324), E341 (= E326), G371 (= G352), D373 (= D354), S374 (≠ T355)

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
46% identity, 96% coverage: 11:393/399 of query aligns to 10:386/392 of 4feyA

query
sites
4feyA

D

D

L

L

K

K

D

D

K

K

K

K

V

V

F

L

I

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

Q

V

D

-

S

K

K

D

G

G

N

K

I

V

T

T

E

S

D

K

T

V

R
|
R

I

I

T

E

G

A

A

A

L

I

P

P

T

T

I

I

R

Q

Y

Y

A

I

I

L

E

D

Q

Q

G

G

A

G

R

A

V

V

I

I

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

-

T

K

E

G

G

E

E

K

Y

N

D

E

S

K

Q

Y

F

S

S

M

L

V

E

P

P

A

V

A

A

K

K

R

A

L

L

S

S

E

E

L

I

L

I

G

N

R

K

K

P

V

V

K

K

Q

F

A

A

S

K

D

D

C

W

F

L

-

D

G

G

E

V

E

D

V

V

T

-

R

-

E

-

I

-

D

-

A

-

M

-

K

K

S

A

G

G

E

E

V

I

L

V

M

M

L

C

E

E

N

N

V

V

R

R

F

F

Y

N

P

S

G

G

E

E

E

K

K

K

N

S

D

T

P

D

K

D

F

L

A

S

Q

K

Q

K

L

I

A

A

N

S

G

L

C

G

Q

D

V

V

Y

F

V

V

N

M

D

D

A

A

F

F

A

A

V

T

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