SitesBLAST
Comparing WP_011735589.1 NCBI__GCF_000015045.1:WP_011735589.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
38% identity, 96% coverage: 3:520/539 of query aligns to 4:517/526 of 3dc2A
- active site: N96 (= N97), R230 (= R231), D254 (= D255), E259 (= E260), H277 (= H281)
- binding serine: Y458 (= Y461), D460 (= D463), R461 (≠ L464), P462 (= P465), G463 (= G466), A464 (≠ M467), L465 (≠ I468), L484 (= L487)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
38% identity, 96% coverage: 3:520/539 of query aligns to 5:516/525 of 3ddnB
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
34% identity, 81% coverage: 19:453/539 of query aligns to 24:456/533 of O43175
- T78 (≠ V73) binding NAD(+)
- R135 (= R130) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ KV 150:151) binding NAD(+)
- D175 (= D170) binding NAD(+)
- T207 (≠ I202) binding NAD(+)
- CAR 234:236 (≠ AAR 229:231) binding NAD(+)
- D260 (= D255) binding NAD(+)
- V261 (= V256) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (= HLGA 281:284) binding NAD(+)
- A373 (≠ L368) to T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- G377 (= G372) to S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- V425 (≠ I419) to M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
7dkmA Phgdh covalently linked to oridonin (see paper)
40% identity, 56% coverage: 2:305/539 of query aligns to 4:303/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V73), A102 (= A101), G148 (= G147), R151 (≠ K150), I152 (≠ V151), Y170 (≠ C169), D171 (= D170), P172 (= P171), I173 (≠ Y172), H202 (= H201), T203 (≠ I202), P204 (= P203), T209 (= T208), C230 (≠ A229), A231 (= A230), R232 (= R231), H279 (= H281), G281 (= G283)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ E12), K17 (≠ A15), I18 (≠ L16), E293 (≠ V295)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
41% identity, 53% coverage: 19:305/539 of query aligns to 16:299/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G149), I148 (≠ V151), Y166 (≠ C169), D167 (= D170), P168 (= P171), I169 (≠ Y172), I170 (≠ V173), H198 (= H201), T199 (≠ I202), L208 (≠ M211), R228 (= R231)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
40% identity, 56% coverage: 2:305/539 of query aligns to 3:302/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I146), G147 (= G147), L148 (= L148), G149 (= G149), R150 (≠ K150), I151 (≠ V151), G152 (= G152), D170 (= D170), H201 (= H201), T202 (≠ I202), P203 (= P203)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
40% identity, 56% coverage: 2:305/539 of query aligns to 3:302/302 of 6rihA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
40% identity, 56% coverage: 2:305/539 of query aligns to 3:302/303 of 6plgA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
40% identity, 56% coverage: 2:305/539 of query aligns to 4:303/305 of 6plfA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
40% identity, 56% coverage: 2:303/539 of query aligns to 2:299/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N97), A100 (= A101), R149 (≠ K150), I150 (≠ V151), Y168 (≠ C169), D169 (= D170), P170 (= P171), I171 (≠ Y172), H200 (= H201), T201 (≠ I202), P202 (= P203), T207 (= T208), C228 (≠ A229), A229 (= A230), R230 (= R231), H277 (= H281), G279 (= G283)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
40% identity, 56% coverage: 2:303/539 of query aligns to 3:300/301 of 6rj5A
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
40% identity, 55% coverage: 2:298/539 of query aligns to 2:294/297 of 6rj3A
2eklA Structure of st1218 protein from sulfolobus tokodaii
38% identity, 58% coverage: 1:310/539 of query aligns to 5:311/312 of 2eklA
- active site: S100 (≠ N97), R232 (= R231), D256 (= D255), E261 (= E260), H282 (= H281)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ V73), S100 (≠ N97), G148 (= G147), G150 (= G149), R151 (≠ K150), I152 (≠ V151), Y170 (≠ C169), D171 (= D170), I172 (≠ P171), L173 (≠ Y172), H202 (= H201), V203 (≠ I202), T204 (≠ P203), I212 (≠ M211), T230 (≠ A229), S231 (≠ A230), D256 (= D255), G284 (= G283)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
41% identity, 50% coverage: 36:303/539 of query aligns to 31:291/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ K150), Y160 (≠ C169), D161 (= D170), P162 (= P171), I164 (≠ V173), L179 (≠ H188), T193 (≠ I202), P194 (= P203), S198 (≠ E207), L202 (≠ M211)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
38% identity, 51% coverage: 33:305/539 of query aligns to 32:302/304 of 1wwkA
- active site: S96 (≠ N97), R230 (= R231), D254 (= D255), E259 (= E260), H278 (= H281)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ A101), G146 (= G147), F147 (≠ L148), G148 (= G149), R149 (≠ K150), I150 (≠ V151), Y168 (≠ C169), D169 (= D170), P170 (= P171), V201 (≠ I202), P202 (= P203), T207 (= T208), T228 (≠ A229), S229 (≠ A230), D254 (= D255), H278 (= H281), G280 (= G283)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 51% coverage: 31:304/539 of query aligns to 34:311/334 of 5aovA
- active site: L100 (≠ N97), R241 (= R231), D265 (= D255), E270 (= E260), H288 (= H281)
- binding glyoxylic acid: M52 (≠ R49), L53 (≠ S50), L53 (≠ S50), Y74 (≠ A71), A75 (≠ G72), V76 (= V73), G77 (= G74), R241 (= R231), H288 (= H281)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V73), T104 (≠ A101), F158 (≠ L148), G159 (= G149), R160 (≠ K150), I161 (≠ V151), S180 (≠ D170), R181 (≠ P171), A211 (≠ H201), V212 (≠ I202), P213 (= P203), T218 (= T208), I239 (≠ A229), A240 (= A230), R241 (= R231), H288 (= H281), G290 (= G283)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 70% coverage: 1:377/539 of query aligns to 56:453/466 of P87228
- S87 (= S32) modified: Phosphoserine
- S258 (≠ N205) modified: Phosphoserine
7cvpA The crystal structure of human phgdh from biortus.
41% identity, 42% coverage: 77:303/539 of query aligns to 31:254/254 of 7cvpA
- binding nicotinamide-adenine-dinucleotide: G101 (= G147), G103 (= G149), R104 (≠ K150), I105 (≠ V151), Y123 (≠ C169), D124 (= D170), P125 (= P171), I126 (≠ Y172), H155 (= H201), T156 (≠ I202), P157 (= P203), T162 (= T208), C183 (≠ A229), A184 (= A230), R185 (= R231), H232 (= H281), G234 (= G283)
5ofwA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 3-chloro-4-fluorobenzamide (see paper)
44% identity, 36% coverage: 96:290/539 of query aligns to 2:193/195 of 5ofwA
- active site: N3 (= N97), R137 (= R231), D161 (= D255), E166 (= E260), H184 (= H281)
- binding 3-chloranyl-4-fluoranyl-benzamide: G53 (= G147), Y75 (≠ C169), P77 (= P171), T108 (≠ I202), S113 (≠ E207), T114 (= T208), L117 (≠ M211)
5ofvA Crystal structure of human 3-phosphoglycerate dehydrogenase in complex with 5-fluoro-2-methylbenzoic acid (see paper)
44% identity, 36% coverage: 96:290/539 of query aligns to 2:193/195 of 5ofvA
Query Sequence
>WP_011735589.1 NCBI__GCF_000015045.1:WP_011735589.1
MRIIVTDEVSAEGLALLTQEPRIQLDVKLGLSKEQLLEILGEYDVIITRSGTSVDRDVLD
AGKRLKIVARAGVGIDNVDVDYASSKGVIVINAPFGNTNSAAEHALALLMTFCRKITIAN
ASLKSGEWKRAPFTGYELRGKVAGVIGLGKVGGRVATRLKAFECEVLACDPYVSVKRAHD
LGVKLVSHDEIYKNCDIITLHIPLNDETRDMISAREFGLMKSGVIVINAARGGIINEQAL
LDNLNSGKVLGGGVDVWSEEPPKSDLLRQLIGHERLVVTPHLGANTHEAQINVAVDVSRE
IVNYLDDQPMESAVNIPRFDMALMDQMRPFLNLMNVICEIGVQLLDNNVDKVSFGYSGSI
AHYDCTPLTVTGLSSLLNRVVDQDVNMVNATLIADQMGIVVEENKSTSCDAFSNVITLII
EGGGKKRTVSGTLFEGAPRIVRLRDFSIDFSPEEHMLLLHYIDLPGMIGRIGTIMGTHDI
NIASMNLGRREKKGEAMVILSLDSAVPLQVVEEIRTATDASFIKPLHLKSVSCSRGRGL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory