SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011735721.1 NCBI__GCF_000015045.1:WP_011735721.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
68% identity, 99% coverage: 1:314/317 of query aligns to 1:312/323 of P0ABK5

query
sites
P0ABK5

M
|
M

S

S

R

K

I

I

F

F

S

E

D

D

N

N

S

S

Q

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

V

I

T

G

D

N

G

G

A

R

R

-

G

-

I

-

V

I

L

L

A

A

K

K

M

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

E

K

R

R

G

G

I

V

L

L

K

K

P

P

G

G

M

V

E

E

I

L

V

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

V

L

A

K

A

A

L

L

G

G

A

A

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

Q

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

N

P

P

E

E

R

K

Y

Y

F

L

L

L

P

L

Q

Q

F

F

K

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

A

Q

I

V

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D

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A

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V

G

G

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T

G

G

T

T

I

L

S

T

G

G

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V

S

S

R

R

Y

Y

I

I

K

K

N

G

S

T

R

K

G

G

K

K

-

T

Q

D

I

L

V

I

S

S

V

V

A

A

V

V

E

E

P

P

Q

T

E

D

S

S

P

P

V

V

I

I

T

A

Q

Q

Q

A

L

L

A

A

G

G

R

E

P

E

L

I

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

A

T

N

L

L

D

D

L

L

S

K

I

L

I

V

D

D

R

K

V

V

E

I

L

G

V

I

E

T

S

N

G

E

E

E

A

A

V

I

D

S

F

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S

S

G

G

A

A

A

V

A

A

V

L

R

K

L

L

A

Q

S

E

L

D

D

E

E

S

F

F

A

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P

P

D

S

L

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

M1 (= M1) modified: Initiator methionine, Removed
K42 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
68% identity, 99% coverage: 1:314/317 of query aligns to 1:312/323 of P0A1E3

query
sites
P0A1E3

M
|
M

S

S

R

K

I

I

F

Y

S

E

D

D

N

N

S

S

Q

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

V

I

T

G

D

N

G

G

A

R

R

-

G

-

I

-

V

I

L

L

A

A

K

K

M

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

I

V

K

K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

E

K

R

R

G

G

I

V

L

L

K

K

P

P

G

G

M

V

E

E

I

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

V

L

A

K

A

A

L

L

G

G

A

A

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

Q

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

Q

R

K

Y

Y

F

L

L

L

P

L

Q

Q

F

F

K

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

A

Q

I

V

D

D

V

V

L

F

V

I

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S

G

G

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G

G

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T

T

I

L

S

T

G

G

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V

S

T

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R

Y

Y

I

I

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K

N

G

S

T

R

K

G

G

K

K

-

T

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V

A

A

V

V

E

E

P

P

Q

T

E

D

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S

P

P

V

V

I

I

T

A

Q

Q

Q

A

L

L

A

A

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E

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E

L

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K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

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P

E

G

T

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L

L

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D

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K

I

L

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K

V

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S

N

G

E

E

E

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A

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I

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T

A

A

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R

L

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

A

V

A

A

V

L

R

K

L

L

A

Q

S

E

L

D

D

E

E

S

F

F

A

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P

P

D

S

L

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

M1 (= M1) modified: Initiator methionine, Removed
N72 (= N75) binding pyridoxal 5'-phosphate
S273 (= S275) binding pyridoxal 5'-phosphate

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
68% identity, 99% coverage: 1:314/317 of query aligns to 2:313/321 of 6z4nAAA

query
sites
6z4nAAA

M

M

S

S

R

K

I

I

F

Y

S

E

D

D

N

N

S

S

Q

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

V

I

T

G

D

N

G

G

A

R

R

-

G

-

I

-

V

I

L

L

A

A

K

K

M

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

E

K

R

R

G

G

I

V

L

L

K

K

P

P

G

G

M

V

E

E

I

L

V

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

V

L

A

K

A

A

L

L

G

G

A

A

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

Q

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

Q

R

K

Y

Y

F

L

L

L

P

L

Q

Q

Q
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Q

F
|
F

K

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

A

Q

I

V

D

D

V

V

L

F

V

I

S

S

G

G

V
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V

G
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G

T
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T

G
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G

G

G

T
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T

I

L

S

T

G

G

I

V

S

T

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R

Y

Y

I

I

K

K

N

G

S

T

R

K

G

G

K

K

-

T

Q

D

I

L

V

I

S

T

V

V

A

A

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V

E

E

P

P

Q

T

E

D

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S

P

P

V

V

I

I

T

A

Q

Q

Q

A

L

L

A

A

G

G

R

E

P

E

L

I

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q
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Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

G

T

N

L

L

D

D

L

L

S

K

I

L

I

I

D

D

R

K

V

V

E

V

L

G

V

I

E

T

S

N

G

E

E

E

A

A

V

I

D

S

F

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
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S

S

S

G

G

A

A

A

V

A

A

V

L

R

K

L

L

A

Q

S

E

L

D

D

E

E

S

F

F

A

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

D

S

L

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

binding pyridoxal-5'-phosphate: K43 (= K45), N73 (= N75), V177 (= V179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G230 (= G231), S274 (= S275), P301 (= P302)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K45), T70 (= T72), G72 (= G74), N73 (= N75), T74 (= T76), Q144 (= Q146), F145 (= F147), Q229 (= Q230), G230 (= G231), I231 (= I232), A233 (= A234)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
67% identity, 99% coverage: 2:314/317 of query aligns to 1:311/322 of 1d6sA

query
sites
1d6sA

S

S

R

K

I

I

F

Y

S

E

D

D

N

N

S

S

Q

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

V

I

T

G

D

N

G

G

A

R

R

-

G

-

I

-

V

I

L

L

A

A

K

K

M

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

I

V

K
x
A

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

E

K

R

R

G

G

I

V

L

L

K

K

P

P

G

G

M

V

E

E

I

L

V

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

V

L

A

K

A

A

L

L

G

G

A

A

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

Q

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

Q

R

K

Y

Y

F

L

L

L

P

L

Q

Q

Q
|
Q

F
|
F

K

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

A

Q

I

V

D

D

V

V

L

F

V

I

S

S

G

G

V

V

G
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G

T
|
T

G
|
G

G

G

T
|
T

I

L

S

T

G

G

I

V

S

T

R

R

Y

Y

I

I

K

K

N

G

S

T

R

K

G

G

K

K

-

T

Q

D

I

L

V

I

S

T

V

V

A

A

V

V

E

E

P

P

Q

T

E

D

S

S

P

P

V

V

I

I

T

A

Q

Q

Q

A

L

L

A

A

G

G

R

E

P

E

L

I

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

G

T

N

L

L

D

D

L

L

S

K

I

L

I

I

D

D

R

K

V

V

E

V

L

G

V

I

E

T

S

N

G

E

E

E

A

A

V

I

D

S

F

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
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S

S

S

G

G

A

A

A

V

A

A

V

L

R

K

L

L

A

Q

S

E

L

D

D

E

E

S

F

F

A

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

D

S

L

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

active site: A41 (≠ K45), G228 (= G231)
binding methionine: T68 (= T72), N69 (≠ S73), N71 (= N75), T72 (= T76), Q142 (= Q146), F143 (= F147), G176 (= G180), G228 (= G231)
binding pyridoxal-5'-phosphate: N71 (= N75), G176 (= G180), T177 (= T181), G178 (= G182), T180 (= T184), G228 (= G231), S272 (= S275), P299 (= P302)

1fcjA Crystal structure of oass complexed with chloride and sulfate (see paper)
67% identity, 95% coverage: 2:302/317 of query aligns to 1:299/302 of 1fcjA

query
sites
1fcjA

S

S

R

K

I

I

F

Y

S

E

D

D

N

N

S

S

Q

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

V

I

T

G

D

N

G

G

A

R

R

-

G

-

I

-

V

I

L

L

A

A

K

K

M

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

E

K

R

R

G

G

I

V

L

L

K

K

P

P

G

G

M

V

E

E

I

L

V

V

E

E

P

P

T

T

S

N

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

V

L

A

K

A

A

L

L

G

G

A

A

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

Q

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

Q

R

K

Y

Y

F

L

L

L

P

L

Q

Q

Q
|
Q

F

F

K

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

A

Q

I

V

D

D

V

V

L

F

V

I

S

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

S

T

G

G

I

V

S

T

R

R

Y

Y

I

I

K

K

N

G

S

T

R

K

G

G

K

K

-

T

Q

D

I

L

V

I

S

T

V

V

A

A

V

V

E

E

P

P

Q

T

E

D

S

S

P

P

V

V

I

I

T

A

Q

Q

Q

A

L

L

A

A

G

G

R

E

P

E

L

I

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

G

T

N

L

L

D

D

L

L

S

K

I

L

I

I

D

D

R

K

V

V

E

V

L

G

V

I

E

T

S

N

G

E

E

E

A

A

V

I

D

S

F

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

A

V

A

A

V

L

R

K

L

L

A

Q

S

E

L

D

D

E

E

S

F

F

A

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

active site: K41 (= K45), G228 (= G231), S272 (= S275)
binding pyridoxal-5'-phosphate: K41 (= K45), N71 (= N75), V175 (= V179), G176 (= G180), T177 (= T181), G178 (= G182), T180 (= T184), G228 (= G231), S272 (= S275), P299 (= P302)
binding sulfate ion: G70 (= G74), T72 (= T76), Q142 (= Q146)

5dbhX Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with reaction intermediate alpha-aminoacrylate
69% identity, 99% coverage: 4:316/317 of query aligns to 2:312/312 of 5dbhX

query
sites
5dbhX

I

I

F

Y

S

A

D

D

N

N

S

S

Q

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

V

F

T

-

D

-

G

G

A

H

R

N

G

G

I

N

V

V

L

V

A

V

K

K

M

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

E

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

M

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

I

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

R

K

R

R

V

L

A

C

A

G

L

L

G

G

A

V

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

S

R

R

Y

Y

F

V

L

M

P

L

Q

K

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

K

D

D

T

T

D

D

G

G

A

K

I

V

D

D

V

V

L

V

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
x
S

I

I

S

T

G

G

I

I

S

S

R

R

Y

A

I

I

K

K

N

L

S

D

R

F

G

G

K

K

Q

Q

I

I

V

T

S

S

V

V

A

A

V

V

E

E

P

P

Q

V

E

E

S

S

P

P

V

V

I

I

T

S

Q

Q

Q

T

L

L

A

A

G

G

R

E

P

E

L

V

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

T

N

L

L

D

D

L

L

S

S

I

I

D

D

R

R

V

V

E

E

L

T

V

V

E

D

S

S

G

D

E

T

A

A

V

L

D

A

F

T

A

A

R

R

L

L

A

M

R

A

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

A

V

A

A

V

D

R

R

L

L

A

A

S

K

L

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

L

A

A

S

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

D

E

G

G

binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: K41 (= K45), T68 (= T72), S69 (= S73), N71 (= N75), T72 (= T76), Q142 (= Q146), G176 (= G180), T177 (= T181), G178 (= G182), S180 (≠ T184), G227 (= G231), S271 (= S275), P298 (= P302)

1y7lA O-acetylserine sulfhydrylase complex (see paper)
69% identity, 98% coverage: 4:314/317 of query aligns to 2:310/310 of 1y7lA

query
sites
1y7lA

I

I

F

Y

S

A

D

D

N

N

S

S

Q

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

V

F

T

-

D

-

G

G

A

H

R

N

G

G

I

N

V

V

L

V

A

V

K

K

M

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

E

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

M

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

I

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

R

K

R

R

V

L

A

C

A

G

L

L

G

G

A

V

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E

K

G

G

M
|
M

K

K

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

S

R

R

Y

Y

F

V

L

M

P

L

Q

K

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

K

D

D

T

T

D

D

G

G

A

K

I

V

D

D

V

V

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

S

T

G

G

I

I

S

S

R

R

Y

A

I

I

K

K

N

L

S

D

R

F

G

G

K

K

Q

Q

I

I

V

T

S

S

V

V

A

A

V

V

E

E

P

P

Q

V

E

E

S

S

P

P

V

V

I

I

T

S

Q

Q

Q

T

L

L

A

A

G

G

R

E

P

E

L

V

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

T

N

L

L

D

D

L

L

S

S

I

I

D

D

R

R

V

V

E

E

L

T

V

V

E

D

S

S

G

D

E

T

A

A

V

L

D

A

F

T

A

A

R

R

L

L

A

M

R

A

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

A

V

A

A

V

D

R

R

L

L

A

A

S

K

L

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

L

A

A

S

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

active site: K41 (= K45), S271 (= S275), P298 (= P302)
binding : K41 (= K45), T68 (= T72), S69 (= S73), T72 (= T76), M119 (= M123), Q142 (= Q146)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
64% identity, 99% coverage: 4:317/317 of query aligns to 4:309/310 of 5xoqA

query
sites
5xoqA

I

I

F

F

S

K

D

D

N

N

S

S

Q

E

S

S

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

L

I

N

N

R

R

V

L

T

T

D

A

G

G

A

L

R

S

G

S

I

R

V

V

L

L

A

A

K

K

M

I

E

E

G

G

R

R

N

N

P

P

A

A

Y

Y

S

S

I

V

K

K

C

C

R

R

I

I

G

G

A

A

N

A

M

M

I

I

W

W

D

D

A

A

E

E

E

Q

R

S

G

G

I

K

L

L

K

K

P

P

G

G

M

M

E

H

I

V

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

F

V

V

A

C

A

A

R

R

G

G

Y

Y

N

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

R

M

V

M

V

L

A

K

A

S

L

F

G

G

A

A

N

D

L

L

I

V

L

L

T

T

P

P

G

G

N

A

E

D

G

G

M
|
M

K

K

G

G

A

A

I

I

R

S

R

K

A

A

E

E

L

L

A

A

S

Q

D

-

P

-

E

P

R

G

Y

W

F

F

L

I

P

P

Q

Q

Q
|
Q

F

F

K

K

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

V

K

K

T

T

G

G

P

P

E

E

I

I

W

W

D

N

D

D

T

T

D

E

G

G

A

Q

I

V

D

D

V

V

L

F

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

S

T

G

G

I

V

S

A

R

R

Y

F

I

L

K

K

N

H

S

E

R

K

G

K

K

H

Q

P

I

V

V

H

S

V

V

V

A

A

V

V

E

E

P

P

Q

A

E

A

S

S

P

P

V

V

I

L

T

A

Q

G

L

P

A

A

G

G

R
|
R

P

-

L

-

Q

-

P

-

G

-

L

-

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

E

D

T

T

L

F

D

D

L

R

S

S

I

V

D

D

R

E

V

I

E

L

L

S

V

V

E

T

S

D

G

D

E

E

A

A

V

I

D

E

F

T

A

A

R

R

R

K

L

L

A

A

R

M

E

E

G

G

M

I

L

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

A

M

A

A

A

G

A

A

V

L

R

K

L

V

A

A

S

A

L

R

D

P

E

E

F

F

A

A

G

G

K

K

T

T

I

I

V

V

V

T

I

V

L

L

P

P

D

D

L

A

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

D

E

G

N

V

L

binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), M123 (= M123), Q144 (= Q146), R218 (= R220), H219 (= H227), Q222 (= Q230), G223 (= G231), A226 (= A234)

4zu6X Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with pre-reactive o-acetyl serine, alpha- aminoacrylate reaction intermediate and peptide inhibitor at the resolution of 2.25a
69% identity, 98% coverage: 4:314/317 of query aligns to 3:309/309 of 4zu6X

query
sites
4zu6X

I

I

F

Y

S

A

D

D

N

N

S

S

Q

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

V

F

T

-

D

-

G

G

A

H

R

N

G

G

I

N

V

V

L

V

A

V

K

K

M

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

E

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

M

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

I

T

T

L

L

T

T

M

M

P

P

E

E

T
|
T

M
|
M

S

S

V

L

E

E

R

R

R

K

R

R

V

L

A

C

A

G

L

L

G

G

A

V

N

N

L

L

I

V

L

L

T

T

P

E

G

G

N

A

E
x
K

G
|
G

M

-

K

-

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

S

R

R

Y

Y

F

V

L

M

P

L

Q

K

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

K

D

D

T

T

D

D

G

G

A

K

I

V

D

D

V

V

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

T
x
S

I

I

S

T

G

G

I

I

S

S

R

R

Y

A

I

I

K

K

N

L

S

D

R

F

G

G

K

K

Q

Q

I

I

V

T

S

S

V

V

A

A

V

V

E

E

P

P

Q

V

E

E

S

S

P

P

V

V

I

I

T

S

Q

Q

Q

T

L

L

A

A

G

G

R

E

P

E

L

V

Q

K

P

P

G

G

L
x
P

H
|
H

K
|
K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

K

T

N

L

L

D

D

L

L

S

S

I

I

D

D

R

R

V

V

E

E

L

T

V

V

E

D

S

S

G

D

E

T

A

A

V

L

D

A

F

T

A

A

R

R

L

L

A

M

R

A

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

A

V

A

A

V

D

R

R

L

L

A

A

S

K

L

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

L

A

A

S

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

binding o-acetylserine: T69 (= T72), S70 (= S73), T73 (= T76), Q141 (= Q146), G175 (= G180), G226 (= G231)
binding pyridoxal-5'-phosphate: K42 (= K45), N72 (= N75), V174 (= V179), G175 (= G180), T176 (= T181), G177 (= G182), G178 (= G183), S179 (≠ T184), G226 (= G231), S270 (= S275), P297 (= P302)
binding : S70 (= S73), T95 (= T98), M96 (= M99), K118 (≠ E121), G119 (= G122), P221 (≠ L226), H222 (= H227), K223 (= K228), A229 (= A234)

4oreX Cyrstal structure of o-acetylserine sulfhydrylase ternary complex from haemophilus influenzae at 2.2 a
69% identity, 98% coverage: 4:314/317 of query aligns to 2:308/308 of 4oreX

query
sites
4oreX

I

I

F

Y

S

A

D

D

N

N

S

S

Q

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

V

F

T

-

D

-

G

G

A

H

R

N

G

G

I

N

V

V

L

V

A

V

K

K

M

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

I

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

E

K

R

D

G

G

I

T

L

L

K

T

P

K

G

G

M

K

E

E

I

I

V

V

E

D

P

A

T
|
T

S

-

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

Y

V

V

A

A

R

R

G

G

Y

Y

N

K

L

I

T

T

L

L

T

T

M

M

P
|
P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

R

K

R

R

V

L

A

C

A

G

L

L

G

G

A

V

N

N

L

L

I

V

L

L

T

T

P

E

G

A

N

-

E
x
K

G
|
G

M
|
M

K

K

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

I

A

V

A

A

S

S

D

D

P

P

E

S

R

R

Y

Y

F

V

L

M

P

L

Q

K

Q

Q

F
|
F

K

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

K

D

D

T

T

D

D

G

G

A

K

I

V

D

D

V

V

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

S

T

G

G

I

I

S

S

R

R

Y

A

I

I

K

K

N

L

S

D

R

F

G

G

K

K

Q

Q

I

I

V

T

S

S

V

V

A

A

V

V

E

E

P

P

Q

V

E

E

S

S

P

P

V

V

I

I

T

S

Q

Q

Q

T

L

L

A

A

G

G

R

E

P

E

L

V

Q

K

P

P

G
|
G

L
x
P

H
|
H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

K

T

N

L

L

D

D

L

L

S

S

I

I

D

D

R

R

V

V

E

E

L

T

V

V

E

D

S

S

G

D

E

T

A

A

V

L

D

A

F

T

A

A

R

R

L

L

A

M

R

A

E

E

G

G

M

I

L

L

V

A

G

G

I

I

S

S

G

G

A

A

A

V

A

A

V

D

R

R

L

L

A

A

S

K

L

L

D

P

E

E

F

F

A

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P

P

D

S

L

A

A

S

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

F

F

binding o-acetylserine: K115 (≠ E121), G116 (= G122), M117 (= M123)
binding : K41 (= K45), T68 (= T72), G69 (= G74), T71 (= T76), P91 (= P96), M117 (= M123), F141 (= F147), G219 (= G225), P220 (≠ L226), H221 (= H227), G225 (= G231), A228 (= A234)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
60% identity, 98% coverage: 4:314/317 of query aligns to 3:305/306 of 2q3dA

query
sites
2q3dA

I

I

F

A

S

E

D

D

N

I

S

T

Q

Q

S

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

N

R

R

R

V

V

T

T

D

D

G

G

A

A

R

V

G

A

I

D

V

I

L

V

A

A

K

K

M

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

I

V

K
|
K

C

D

R

R

I

I

G

G

A

V

N

A

M

M

I

L

W

Q

D

A

A

A

E

E

E

Q

R

A

G

G

I

L

L

I

K

K

P

P

G

D

M

T

E

I

I

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

C

A

A

R

R

G

G

Y

Y

N

R

L

C

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

R

M

V

L

A

R

A

A

L

Y

G

G

A

A

N

E

L

L

I

I

L

L

T

T

P

P

G

G

N

A

E

D

G

G

M

M

K

S

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

L

A

A

A

K

S

T

D

D

P

-

E

Q

R

R

Y

Y

F

F

L

V

P

P

Q

Q

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

D

R

D

D

T

T

D

D

G

G

A

K

I

V

D

D

V

I

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

I

V

S

A

R

Q

Y

V

I

I

K

K

N

E

S

-

R

R

G

K

K

P

Q

S

I

A

V

R

S

F

V

V

A

A

V

V

E

E

P

P

Q

A

E

A

S

S

P

P

V

V

I

L

T

S

Q

G

L

-

A

-

G

-

R

-

P

-

L

-

Q

Q

P

K

G

G

L

P

H

H

K

P

I

I

Q

Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

I

V

P

P

E

P

T

V

L

L

D

D

L

Q

S

D

I

L

I

V

D

D

R

E

V

I

E

I

L

T

V

V

E

G

S

N

G

E

E

D

A

A

V

L

D

N

F

V

A

A

R

R

L

L

A

A

R

R

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

A

T

A

V

A

A

V

L

R

Q

L

V

A

A

S

R

L

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P
|
P

D
|
D

L

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

active site: K44 (= K45), S266 (= S275), P293 (= P302)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K45), T71 (= T72), S72 (= S73), N74 (= N75), T75 (= T76), Q144 (= Q146), V177 (= V179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G222 (= G231), I223 (= I232), S266 (= S275), P293 (= P302), D294 (= D303)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
60% identity, 99% coverage: 4:317/317 of query aligns to 3:308/310 of P9WP55

query
sites
P9WP55

I

I

F

A

S

E

D

D

N

I

S

T

Q

Q

S

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

N

R

R

R

V

V

T

T

D

D

G

G

A

A

R

V

G

A

I

D

V

I

L

V

A

A

K

K

M

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

I

V

K
|
K

C

D

R

R

I

I

G

G

A

V

N

A

M

M

I

L

W

Q

D

A

A

A

E

E

E

Q

R

A

G

G

I

L

L

I

K

K

P

P

G

D

M

T

E

I

I

I

V

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

Y

M

V

V

A

C

A

A

R

R

G

G

Y

Y

N

R

L

C

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

R

M

V

L

A

R

A

A

L

Y

G

G

A

A

N

E

L

L

I

I

L

L

T

T

P

P

G

G

N

A

E

D

G

G

M

M

K

S

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

L

A

A

A

K

S

T

D

D

P

-

E

Q

R

R

Y

Y

F

F

L

V

P

P

Q

Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

D

R

D

D

T

T

D

D

G

G

A

K

I

V

D

D

V

I

L

V

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

T

G

G

I

V

S

A

R

Q

Y

V

I

I

K

K

N

E

S

-

R

R

G

K

K

P

Q

S

I

A

V

R

S

F

V

V

A

A

V

V

E

E

P

P

Q

A

E

A

S

S

P

P

V

V

I

L

T

S

Q

G

L

-

A

-

G

-

R

-

P

-

L

-

Q

Q

P

K

G

G

L

P

H

H

K

P

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

E

P

T

V

L

L

D

D

L

Q

S

D

I

L

I

V

D

D

R

E

V

I

E

I

L

T

V

V

E

G

S

N

G

E

E

D

A

A

V

L

D

N

F

V

A

A

R

R

L

L

A

A

R

R

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

A

T

A

V

A

A

V

L

R

Q

L

V

A

A

S

R

L

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P

P

D

D

L

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

D

A

G

D

V

V

K44 (= K45) modified: N6-(pyridoxal phosphate)lysine
N74 (= N75) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
S266 (= S275) binding pyridoxal 5'-phosphate

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
59% identity, 97% coverage: 4:309/317 of query aligns to 3:300/300 of 3zeiA

query
sites
3zeiA

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active site: K44 (= K45), S266 (= S275), P293 (= P302)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T72), S72 (= S73), I126 (= I127), Q144 (= Q146), F145 (= F147), K215 (≠ P224), G222 (= G231), A225 (= A234), F227 (= F236)
binding pyridoxal-5'-phosphate: K44 (= K45), N74 (= N75), V177 (= V179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G222 (= G231), S266 (= S275), P293 (= P302), D294 (= D303)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
59% identity, 97% coverage: 4:309/317 of query aligns to 3:300/300 of 2q3cA

query
sites
2q3cA

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active site: K44 (= K45), S266 (= S275), P293 (= P302)
binding : T71 (= T72), S72 (= S73), G73 (= G74), T75 (= T76), M122 (= M123), Q144 (= Q146), K215 (≠ P224), G222 (= G231), A225 (= A234)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
56% identity, 99% coverage: 3:317/317 of query aligns to 2:311/318 of 4lmaA

query
sites
4lmaA

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active site: K46 (= K45), S269 (= S275)
binding pyridoxal-5'-phosphate: K46 (= K45), N77 (= N75), G181 (= G180), T182 (= T181), G183 (= G182), T185 (= T184), G225 (= G231), S269 (= S275), P296 (= P302)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
55% identity, 99% coverage: 2:315/317 of query aligns to 1:307/320 of 2isqA

query
sites
2isqA

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D

active site: K44 (= K45), S267 (= S275)
binding pyridoxal-5'-phosphate: K44 (= K45), N75 (= N75), G177 (= G178), G179 (= G180), T180 (= T181), G181 (= G182), T183 (= T184), G223 (= G231), S267 (= S275), P294 (= P302)
binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), G122 (= G122), M123 (= M123), K124 (= K124), G217 (= G225), P218 (≠ L226), H219 (= H227), Q222 (= Q230), G223 (= G231)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
55% identity, 99% coverage: 2:315/317 of query aligns to 3:309/322 of P47998

query
sites
P47998

S

S

R

R

I

I

F

A

S

K

D

D

N

V

S

T

Q

E

S

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

N

N

R

N

V

V

T

A

D

E

G

G

A

C

R

V

G

G

I

R

V

V

L

A

A

A

K

K

M

L

E

E

G

M

R

M

N

E

P

P

A

C

Y

S

S

S

I

V

K
|
K

C

D

R

R

I

I

G

G

A

F

N

S

M

M

I

I

W

S

D

D

A

A

E

E

E

K

R

K

G

G

I

L

L

I

K

K

P

P

G

G

M

E

E

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Y

F

V

T

A

A

R

K

G

G

Y

Y

N

K

L

L

T

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

R

I

V

I

V

L

A

L

A

A

L

F

G

G

A

V

N

E

L

L

I

V

L

L

T

T

P

D

G

P

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

I

A

L

A

A

S

K

D

T

P

P

E

N

R

G

Y

Y

F

M

L

L

P

-

Q

Q

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H
|
H

E

Y

K

E

T

T

G
|
G

P

P

E

E

I

I

W

W

D

K

D

G

T

T

D

G

G

G

A

K

I

I

D

D

V

G

L

F

V

V

S

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

T

G

G

I

A

S

G

R

K

Y

Y

I

L

K

K

N

E

S

-

R

Q

G

N

K

A

Q

N

I

V

V

K

S

L

V

Y

A

G

V

V

E

E

P

P

Q

V

E

E

S

S

P

A

V

I

I

L

T

S

Q

G

L

-

A

-

G

-

R

-

P

-

L

-

Q
x
K

P

P

G

G

L

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

S

T

V

L

L

D

N

L

V

S

D

I

L

I

I

D

D

R

E

V

V

E

V

L

Q

V

V

E

S

S

S

G

D

E

E

A

S

V

I

D

D

F

M

A

A

R

R

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

I

R

K

L

L

A

A

S

Q

L

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P

P

D

S

L

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

D

D

K46 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T72) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S73) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N75) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T76) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q146) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H156) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G161) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
T182 (= T181) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T184) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ Q223) mutation to A: Impaired interaction with SAT1.
H221 (= H227) mutation to A: Impaired interaction with SAT1.
K222 (= K228) mutation to A: Impaired interaction with SAT1.
S269 (= S275) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
54% identity, 99% coverage: 5:317/317 of query aligns to 13:319/323 of 4aecA

query
sites
4aecA

F

I

S

A

D

D

N

N

-

V

S

S

Q

Q

S

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

N

N

R

S

V

I

T

A

D

K

G

G

A

C

R

V

G

A

I

N

V

I

L

A

A

A

K

K

M

L

E

E

G

I

R

M

N

E

P

P

A

C

Y

C

S

S

I

V

K
|
K

C

D

R

R

I

I

G

G

A

Y

N

S

M

M

I

V

W

T

D

D

A

A

E

E

E

Q

R

K

G

G

I

F

L

I

K

S

P

P

G

G

M

K

E

S

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

Y

F

V

I

A

A

A

S

R

R

G

G

Y

Y

N

R

L

L

T

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

M

E

E

R

R

R

V

V

L

A

K

A

A

L

F

G

G

A

A

N

E

L

L

I

V

L

L

T

T

P

D

G

P

N

A

E

K

G

G

M

M

K

T

G

G

A

A

I

V

R

Q

R

K

A

A

E

E

L

I

A

L

A

K

S

N

D

T

P

P

E

D

R

A

Y

Y

F

M

L

L

P

-

Q

Q

F

F

K

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

D

T

T

D

K

G

G

A

K

I

V

D

D

V

I

L

F

V

V

S

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

T

G

G

I

V

S

G

R

R

Y

F

I

I

K

K

N

E

S

K

R

N

G

P

K

K

Q

T

I

Q

V

V

S

-

V

I

A

G

V

V

E

E

P

P

Q

T

E

E

S

S

P

D

V

I

I

L

T

S

Q

-

L

-

A

G

G

G

R

K

P

P

L

-

Q

-

P

-

G

G

L

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

T

N

L

L

D

D

L

Q

S

K

I

I

I

M

D

D

R

E

V

V

E

I

L

A

V

I

E

S

S

S

G

E

E

E

A

A

V

I

D

E

F

T

A

A

R

K

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

M

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

I

R

K

L

V

A

A

S

K

L

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

A

V

V

I

V

L

F

P
|
P

D

S

L

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

F

F

D

Q

G

S

V

I

active site: K54 (= K45), S277 (= S275)
binding pyridoxal-5'-phosphate: K54 (= K45), N85 (= N75), I188 (≠ V179), G189 (= G180), T190 (= T181), G191 (= G182), G192 (= G183), T193 (= T184), G233 (= G231), S277 (= S275), P304 (= P302)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
55% identity, 99% coverage: 2:315/317 of query aligns to 1:307/320 of 1z7yA

query
sites
1z7yA

S

S

R

R

I

I

F

A

S

K

D

D

N

V

S

T

Q

E

S

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

N

N

R

N

V

V

T

A

D

E

G

G

A

C

R

V

G

G

I

R

V

V

L

A

A

A

K

K

M

L

E

E

G

M

R

M

N

E

P

P

A

C

Y

S

S

S

I

V

K
x
A

C

D

R

R

I

I

G

G

A

F

N

S

M

M

I

I

W

S

D

D

A

A

E

E

E

K

R

K

G

G

I

L

L

I

K

K

P

P

G

G

M

E

E

S

I

V

-

L

V

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

Y

F

V

T

A

A

R

K

G

G

Y

Y

N

K

L

L

T

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

R

I

V

I

V

L

A

L

A

A

L

F

G

G

A

V

N

E

L

L

I

V

L

L

T

T

P

D

G

P

N

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

R

A

R

K

A

A

E

E

L

I

A

L

A

A

S

K

D

T

P

P

E

N

R

G

Y

Y

F

M

L

L

P

-

Q

Q

Q
|
Q

F

F

K

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

K

D

G

T

T

D

G

G

G

A

K

I

I

D

D

V

G

L

F

V

V

S

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

I

A

S

G

R

K

Y

Y

I

L

K

K

N

E

S

-

R

Q

G

N

K

A

Q

N

I

V

V

K

S

L

V

Y

A

G

V

V

E

E

P

P

Q

V

E

E

S

S

P

A

V

I

I

L

T

S

Q

G

L

-

A

-

G

-

R

-

P

-

L

-

Q

K

P

P

G

G

L

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

S

T

V

L

L

D

N

L

V

S

D

I

L

I

I

D

D

R

E

V

V

E

V

L

Q

V

V

E

S

S

S

G

D

E

E

A

S

V

I

D

D

F

M

A

A

R

R

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

I

R

K

L

L

A

A

S

Q

L

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P
|
P

D
x
S

L

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

F

F

D

D

active site: A44 (≠ K45), S267 (= S275)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G74), N75 (= N75), T76 (= T76), Q145 (= Q146), I178 (≠ V179), G179 (= G180), T180 (= T181), G181 (= G182), T183 (= T184), G223 (= G231), S267 (= S275), P294 (= P302), S295 (≠ D303)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
54% identity, 98% coverage: 6:317/317 of query aligns to 76:381/392 of P47999

query
sites
P47999

S

A

D

D

N

N

S

A

-

A

Q

Q

S

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

N

N

R

N

V

V

T

V

D

K

G

G

A

C

R

V

G

A

I

S

V

V

L

A

A

A

K

K

M

L

E

E

G

I

R

M

N

E

P

P

A

C

Y

C

S

S

I

V

K

K

C

D

R

R

I

I

G

G

A

Y

N

S

M

M

I

I

W

T

D

D

A

A

E

E

R

K

G

G

I

L

L

I

K

T

P

P

G

G

M

K

E

S

I

V

-

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

Y

F

V

I

A

A

A

S

R

K

G

G

Y

Y

N

K

L

L

T

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

L

E

E

R

R

R

V

V

L

A

R

A

A

L

F

G

G

A

A

N

E

L

L

I

V

L

L

T

T

P

E

G

P

N

A

E

K

G

G

M

M

K

T

G

G

A

A

I

I

R

Q

R

K

A

A

E

E

L

I

A

L

A

K

S

K

D

T

P

P

E

N

R

S

Y

Y

F

M

L

L

P

-

Q

Q

F

F

K

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

D

E

D

D

T

T

D

R

G

G

A

K

I

I

D

D

V

I

L

L

V

V

S

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

S

T

G

G

I

V

S

G

R

R

Y

F

I

I

K

K

N

E

S

-

R

R

G

K

K

P

Q

E

I

L

V

K

S

V

V

I

A

G

V

V

E

E

P

P

Q

T

E

E

S

S

P

A

V

I

I

L

T

S

Q

-

L

-

A

G

G

G

R

K

P

P

L

-

Q

-

P

-

G

G

L

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

E

K

T

N

L

L

D

D

L

L

S

A

I

I

I

V

D

D

R

E

V

Y

E

I

L

A

V

I

E

S

S

S

G

E

E

E

A

A

V

I

D

E

F

T

A

S

R

K

R

Q

L

L

A

A

R

L

E

Q

E

E

G

G

M

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

V

I

R

Q

L

V

A

A

S

K

L

R

D

P

E

E

F

N

A

A

G

G

K

K

T

L

I

I

V

A

V

V

I

V

L

F

P

P

D

S

L

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

Q

L

L

F

F

D

Q

G

S

V

I

Sites not aligning to the query:

61 modified: N-acetylalanine

Query Sequence

>WP_011735721.1 NCBI__GCF_000015045.1:WP_011735721.1
MSRIFSDNSQSIGNTPLVRLNRVTDGARGIVLAKMEGRNPAYSIKCRIGANMIWDAEERG
ILKPGMEIVEPTSGNTGIALAYVAAARGYNLTLTMPETMSVERRRVVAALGANLILTPGN
EGMKGAIRRAEELAASDPERYFLPQQFKNPANPAIHEKTTGPEIWDDTDGAIDVLVSGVG
TGGTISGISRYIKNSRGKQIVSVAVEPQESPVITQQLAGRPLQPGLHKIQGIGAGFIPET
LDLSIIDRVELVESGEAVDFARRLAREEGMLVGISSGAAAAAAVRLASLDEFAGKTIVVI
LPDLAERYLSTPLFDGV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory