SitesBLAST
Comparing WP_011736419.1 NCBI__GCF_000015045.1:WP_011736419.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
26% identity, 98% coverage: 3:566/573 of query aligns to 6:523/539 of 6lpiB
- active site: I27 (≠ V24), G29 (= G26), G30 (≠ S27), S31 (≠ P28), I32 (≠ L29), E53 (= E53), C76 (≠ T76), F115 (= F115), Q116 (= Q116), E117 (≠ D117), K165 (≠ I170), M256 (≠ V268), A283 (≠ E297), V375 (≠ N414), G401 (≠ A440), M403 (= M442), D428 (= D467), N455 (≠ D494), A457 (= A496), L458 (≠ Y497), L460 (= L499), V461 (≠ I500), Q464 (≠ G503)
- binding flavin-adenine dinucleotide: R155 (≠ P160), G212 (= G224), G213 (≠ A225), G214 (≠ N226), T236 (= T248), L237 (≠ P249), M238 (≠ R250), L254 (≠ F266), M256 (≠ V268), H257 (≠ S269), G276 (= G290), A277 (≠ S291), R278 (≠ N292), D280 (≠ G294), R282 (≠ W296), A283 (≠ E297), D300 (= D314), I301 (≠ S315), D319 (≠ G333), V320 (≠ I334), M380 (≠ P419), G398 (≠ I437)
- binding magnesium ion: D428 (= D467), N455 (≠ D494)
- binding thiamine diphosphate: E53 (= E53), C76 (≠ T76), P79 (= P79), G376 (≠ S415), Q377 (≠ N416), H378 (≠ S417), G401 (≠ A440), M403 (= M442), G427 (= G466), D428 (= D467), G429 (= G468), S430 (≠ C469), M433 (= M472), N455 (≠ D494), A457 (= A496), L458 (≠ Y497), G459 (= G498), L460 (= L499), V461 (≠ I500)
P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
27% identity, 98% coverage: 6:566/573 of query aligns to 94:639/664 of P09114
- P191 (≠ R106) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
- W568 (vs. gap) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.
P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
27% identity, 98% coverage: 6:566/573 of query aligns to 97:642/667 of P09342
- C161 (= C73) modified: Disulfide link with 307
- P194 (≠ R106) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
- C307 (≠ A227) modified: Disulfide link with 161
3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
26% identity, 97% coverage: 6:560/573 of query aligns to 14:553/582 of 3ea4A
- active site: Y32 (≠ V24), G34 (= G26), G35 (≠ S27), A36 (≠ P28), S37 (≠ L29), E58 (= E53), T81 (= T76), F120 (= F115), Q121 (= Q116), E122 (≠ D117), K170 (≠ I170), M265 (≠ V268), V292 (≠ E297), V399 (vs. gap), G425 (≠ A440), M427 (= M442), D452 (= D467), N479 (≠ D494), H481 (≠ A496), L482 (≠ Y497), M484 (≠ L499), V485 (≠ I500), W488 (vs. gap)
- binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ E295), R291 (≠ W296), W488 (vs. gap)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (≠ P160), G221 (= G224), G222 (≠ A225), G223 (≠ N226), T245 (= T248), L246 (≠ P249), M247 (≠ R250), L263 (≠ F266), G264 (= G267), M265 (≠ V268), H266 (= H271), G285 (= G290), R287 (≠ N292), D289 (≠ G294), R291 (≠ W296), D309 (= D314), I310 (≠ S315), G327 (= G332), D328 (≠ G333), V329 (≠ I334), M404 (≠ P419), G422 (≠ I437)
- binding magnesium ion: D452 (= D467), N479 (≠ D494), H481 (≠ A496)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (vs. gap), G400 (≠ S415), Q401 (≠ N416), H402 (≠ S417), M427 (= M442), G451 (= G466), D452 (= D467), G453 (= G468), S454 (≠ C469), N479 (≠ D494), H481 (≠ A496), L482 (≠ Y497), G483 (= G498), M484 (≠ L499), V485 (≠ I500)
Sites not aligning to the query:
3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
26% identity, 97% coverage: 6:560/573 of query aligns to 14:553/582 of 3e9yA
- active site: Y32 (≠ V24), G34 (= G26), G35 (≠ S27), A36 (≠ P28), S37 (≠ L29), E58 (= E53), T81 (= T76), F120 (= F115), Q121 (= Q116), E122 (≠ D117), K170 (≠ I170), M265 (≠ V268), V292 (≠ E297), V399 (vs. gap), G425 (≠ A440), M427 (= M442), D452 (= D467), N479 (≠ D494), H481 (≠ A496), L482 (≠ Y497), M484 (≠ L499), V485 (≠ I500), W488 (vs. gap)
- binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ E295), R291 (≠ W296), W488 (vs. gap)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (≠ P160), G221 (= G224), G222 (≠ A225), G223 (≠ N226), T245 (= T248), L246 (≠ P249), M247 (≠ R250), L263 (≠ F266), G285 (= G290), R287 (≠ N292), D289 (≠ G294), R291 (≠ W296), D309 (= D314), I310 (≠ S315), G327 (= G332), D328 (≠ G333), V329 (≠ I334), M404 (≠ P419), G422 (≠ I437)
- binding magnesium ion: D452 (= D467), N479 (≠ D494), H481 (≠ A496)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (vs. gap), G400 (≠ S415), Q401 (≠ N416), H402 (≠ S417), M427 (= M442), G451 (= G466), G453 (= G468), S454 (≠ C469), N479 (≠ D494), H481 (≠ A496), L482 (≠ Y497), G483 (= G498), M484 (≠ L499), V485 (≠ I500)
Sites not aligning to the query:
7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
26% identity, 97% coverage: 6:560/573 of query aligns to 15:554/582 of 7tzzA
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ V268), R292 (≠ W296), W489 (vs. gap)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (vs. gap), G401 (≠ S415), Q402 (≠ N416), H403 (≠ S417), G426 (≠ A440), M428 (= M442), G452 (= G466), D453 (= D467), G454 (= G468), S455 (≠ C469), L483 (≠ Y497), G484 (= G498), M485 (≠ L499), V486 (≠ I500)
- binding flavin-adenine dinucleotide: R161 (≠ P160), G222 (= G224), G223 (≠ A225), G224 (≠ N226), T246 (= T248), L247 (≠ P249), M248 (≠ R250), M263 (≠ V265), L264 (≠ F266), M266 (≠ V268), H267 (= H271), G286 (= G290), R288 (≠ N292), V293 (≠ E297), D310 (= D314), I311 (≠ S315), D329 (≠ G333), V330 (≠ I334), M405 (≠ P419), G423 (≠ I437)
- binding magnesium ion: A37 (≠ P28), T82 (= T76), S83 (≠ T77), Q122 (= Q116), Y381 (≠ V396), D453 (= D467), M458 (= M472), Q461 (= Q475), N480 (≠ D494), H482 (≠ A496), K533 (≠ I538)
Sites not aligning to the query:
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
26% identity, 97% coverage: 6:560/573 of query aligns to 15:554/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (vs. gap), G401 (≠ S415), Q402 (≠ N416), H403 (≠ S417), G426 (≠ A440), M428 (= M442), G452 (= G466), D453 (= D467), G454 (= G468), S455 (≠ C469), M458 (= M472), N480 (≠ D494), H482 (≠ A496), L483 (≠ Y497), G484 (= G498), M485 (≠ L499), V486 (≠ I500)
- binding flavin-adenine dinucleotide: R161 (≠ P160), G222 (= G224), G223 (≠ A225), G224 (≠ N226), T246 (= T248), L247 (≠ P249), M248 (≠ R250), L264 (≠ F266), M266 (≠ V268), H267 (= H271), G286 (= G290), V287 (≠ S291), R288 (≠ N292), D290 (≠ G294), R292 (≠ W296), V293 (≠ E297), D310 (= D314), I311 (≠ S315), D329 (≠ G333), V330 (≠ I334), M405 (≠ P419), G423 (≠ I437)
- binding magnesium ion: F370 (≠ Y369), D453 (= D467), M458 (= M472), Q461 (= Q475), N480 (≠ D494), H482 (≠ A496), K533 (≠ I538)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ V268), R292 (≠ W296), M485 (≠ L499), W489 (vs. gap)
Sites not aligning to the query:
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
26% identity, 97% coverage: 6:560/573 of query aligns to 15:554/582 of 5wj1A