SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_011736503.1 NCBI__GCF_000015045.1:WP_011736503.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
37% identity, 86% coverage: 1:128/149 of query aligns to 5:134/168 of 6addA

query
sites
6addA
M
 
V
L
 
V
R
 
R
K
 
R
A
 
A
Q
 
R
I
 
T
S
 
S
D
 
D
V
 
V
K
 
P
N
 
A
I
 
I
Q
 
K
K
 
Q
L
 
L
V
 
V
M
 
D
A
 
T
Y
|
Y
A
 
A
T
 
G
K
|
K
G
 
-
D
x
I
M
 
L
L
|
L
S
 
E
R
 
K
S
 
N
L
 
L
S
 
V
E
 
T
L
 
L
Y
 
Y
E
 
E
S
 
A
L
 
V
R
 
Q
D
 
E
F
 
F
Y
 
W
V
 
V
Y
 
A
E
 
E
E
 
H
E
 
P
-
 
D
-
 
L
-
 
Y
G
 
G
K
 
K
L
 
V
L
 
V
G
 
G
T
 
C
T
 
G
A
 
A
L
 
L
H
 
H
I
 
V
V
 
L
W
 
W
E
 
S
D
 
D
L
 
L
A
 
G
E
 
E
V
 
I
R
 
R
S
x
T
T
x
V
A
|
A
V
|
V
A
 
D
E
 
P
E
 
A
A
 
M
T
|
T
R
x
G
R
x
H
G
|
G
I
 
I
G
|
G
G
x
H
K
 
A
L
 
I
V
 
V
Q
 
D
A
 
R
C
 
L
I
 
L
D
 
Q
E
 
V
G
 
A
R
 
R
K
 
D
L
 
L
G
 
Q
L
 
L
K
 
Q
R
 
R
L
 
V
F
 
F
C
 
V
L
|
L
T
|
T
Y
 
F
R
x
E
P
 
T
D
 
E
F
|
F
F
 
F
S
 
A
S
 
R
L
 
H
G
 
G
F
 
F
R
 
T
L
 
E
V
 
I
D
 
E
K
 
G
S
 
T
E
 
P
L
 
V
P
 
T
H
 
A
K
 
E
V
 
V
W
 
F

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
37% identity, 86% coverage: 1:128/149 of query aligns to 5:134/168 of 5yo2A

query
sites
5yo2A
M
 
V
L
 
V
R
 
R
K
 
R
A
 
A
Q
 
R
I
 
T
S
 
S
D
 
D
V
 
V
K
 
P
N
 
A
I
 
I
Q
 
K
K
 
Q
L
 
L
V
 
V
M
 
D
A
 
T
Y
|
Y
A
 
A
T
x
G
K
 
K
G
 
-
D
x
I
M
x
L
L
|
L
S
 
E
R
 
K
S
 
N
L
 
L
S
 
V
E
 
T
L
 
L
Y
 
Y
E
 
E
S
 
A
L
 
V
R
 
Q
D
 
E
F
 
F
Y
 
W
V
 
V
Y
 
A
E
 
E
E
 
H
E
 
P
-
 
D
-
 
L
-
 
Y
G
 
G
K
 
K
L
 
V
L
 
V
G
 
G
T
 
C
T
 
G
A
 
A
L
 
L
H
 
H
I
 
V
V
 
L
W
 
W
E
 
S
D
 
D
L
 
L
A
 
G
E
 
E
V
x
I
R
|
R
S
x
T
T
x
V
A
 
A
V
|
V
A
 
D
E
 
P
E
 
A
A
 
M
T
|
T
R
x
G
R
x
H
G
|
G
I
 
I
G
|
G
G
x
H
K
 
A
L
 
I
V
 
V
Q
 
D
A
 
R
C
 
L
I
 
L
D
 
Q
E
 
V
G
 
A
R
 
R
K
 
D
L
 
L
G
 
Q
L
 
L
K
 
Q
R
 
R
L
 
V
F
 
F
C
 
V
L
|
L
T
|
T
Y
 
F
R
x
E
P
 
T
D
 
E
F
|
F
F
|
F
S
 
A
S
 
R
L
 
H
G
 
G
F
 
F
R
 
T
L
 
E
V
 
I
D
 
E
K
 
G
S
 
T
E
 
P
L
 
V
P
 
T
H
 
A
K
 
E
V
 
V
W
 
F

Sites not aligning to the query:

5ygeA Arga complexed with acecoa and glutamate (see paper)
37% identity, 86% coverage: 1:128/149 of query aligns to 6:135/169 of 5ygeA

query
sites
5ygeA
M
 
V
L
 
V
R
 
R
K
 
R
A
 
A
Q
 
R
I
 
T
S
 
S
D
 
D
V
 
V
K
 
P
N
 
A
I
 
I
Q
 
K
K
 
Q
L
 
L
V
 
V
M
 
D
A
 
T
Y
|
Y
A
 
A
T
 
G
K
 
K
G
 
-
D
 
I
M
 
L
L
 
L
S
 
E
R
x
K
S
 
N
L
 
L
S
 
V
E
 
T
L
 
L
Y
 
Y
E
 
E
S
 
A
L
 
V
R
 
Q
D
x
E
F
 
F
Y
 
W
V
 
V
Y
 
A
E
 
E
E
 
H
E
 
P
-
 
D
-
 
L
-
 
Y
G
 
G
K
 
K
L
 
V
L
 
V
G
 
G
T
 
C
T
 
G
A
 
A
L
 
L
H
|
H
I
 
V
V
 
L
W
 
W
E
 
S
D
 
D
L
 
L
A
 
G
E
 
E
V
x
I
R
|
R
S
 
T
T
x
V
A
|
A
V
|
V
A
 
D
E
 
P
E
 
A
A
 
M
T
|
T
R
x
G
R
x
H
G
|
G
I
 
I
G
|
G
G
x
H
K
 
A
L
 
I
V
 
V
Q
 
D
A
 
R
C
 
L
I
 
L
D
 
Q
E
 
V
G
 
A
R
 
R
K
 
D
L
 
L
G
 
Q
L
 
L
K
 
Q
R
 
R
L
 
V
F
 
F
C
 
V
L
 
L
T
|
T
Y
 
F
R
x
E
P
 
T
D
 
E
F
 
F
F
|
F
S
 
A
S
 
R
L
 
H
G
 
G
F
 
F
R
 
T
L
 
E
V
 
I
D
 
E
K
 
G
S
 
T
E
 
P
L
 
V
P
 
T
H
 
A
K
 
E
V
 
V
W
 
F

Sites not aligning to the query:

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
33% identity, 83% coverage: 2:124/149 of query aligns to 277:401/424 of 4i49A

query
sites
4i49A
L
 
I
R
 
R
K
 
Q
A
 
A
Q
 
H
I
 
S
S
 
G
D
 
D
V
 
I
K
 
P
N
 
H
I
 
I
Q
 
A
K
 
A
L
 
L
V
 
I
M
 
R
A
 
P
Y
 
L
A
 
E
T
 
E
K
 
Q
G
 
G
D
x
I
M
x
L
L
|
L
S
 
H
R
|
R
S
 
S
L
 
R
S
 
E
E
 
Y
L
 
L
Y
 
E
E
 
N
S
 
H
L
 
I
R
 
S
D
 
E
F
 
F
Y
 
S
V
 
I
Y
 
L
E
 
E
E
 
H
E
 
D
G
 
G
K
 
N
L
 
L
L
 
Y
G
 
G
T
 
C
T
 
A
A
 
A
L
 
L
H
 
K
I
 
T
V
 
F
W
 
A
E
 
E
-
 
A
D
 
D
L
 
C
A
 
G
E
 
E
V
 
I
R
x
A
S
x
C
T
x
L
A
|
A
V
|
V
A
 
S
E
 
P
E
 
Q
A
 
A
T
x
Q
R
x
D
R
 
G
G
|
G
I
 
Y
G
|
G
G
x
E
K
 
R
L
 
L
V
 
L
Q
 
A
A
 
H
C
 
I
I
 
I
D
 
D
E
 
K
G
 
A
R
 
R
K
 
G
L
 
I
G
 
G
L
 
I
K
 
S
R
 
R
L
 
L
F
 
F
C
 
A
L
|
L
-
x
S
T
 
T
Y
 
N
R
x
T
P
 
G
D
 
E
F
x
W
F
|
F
S
 
A
S
 
E
L
 
R
G
 
G
F
 
F
R
 
Q
L
 
T
V
 
A
D
 
S
K
 
E
S
 
D
E
 
E
L
 
L
P
 
P

Sites not aligning to the query:

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
33% identity, 83% coverage: 2:124/149 of query aligns to 277:401/424 of 3d2mA

query
sites
3d2mA
L
 
I
R
 
R
K
 
Q
A
 
A
Q
 
H
I
 
S
S
 
G
D
 
D
V
 
I
K
 
P
N
 
H
I
 
I
Q
 
A
K
 
A
L
 
L
V
 
I
M
 
R
A
 
P
Y
 
L
A
 
E
T
 
E
K
 
Q
G
 
G
D
 
I
M
 
L
L
|
L
S
 
H
R
|
R
S
 
S
L
 
R
S
 
E
E
 
Y
L
 
L
Y
 
E
E
 
N
S
 
H
L
 
I
R
 
S
D
 
E
F
 
F
Y
 
S
V
 
I
Y
 
L
E
 
E
E
 
H
E
 
D
G
 
G
K
 
N
L
 
L
L
 
Y
G
 
G
T
 
C
T
 
A
A
 
A
L
 
L
H
 
K
I
 
T
V
 
F
W
 
A
E
 
E
-
 
A
D
 
D
L
 
C
A
 
G
E
 
E
V
 
I
R
x
A
S
x
C
T
x
L
A
 
A
V
|
V
A
 
S
E
 
P
E
 
Q
A
 
A
T
x
Q
R
x
D
R
 
G
G
|
G
I
 
Y
G
|
G
G
x
E
K
 
R
L
 
L
V
 
L
Q
 
A
A
 
H
C
 
I
I
 
I
D
 
D
E
 
K
G
 
A
R
 
R
K
 
G
L
 
I
G
 
G
L
 
I
K
 
S
R
 
R
L
 
L
F
 
F
C
 
A
L
|
L
-
x
S
T
 
T
Y
 
N
R
x
T
P
 
G
D
 
E
F
x
W
F
 
F
S
 
A
S
 
E
L
 
R
G
 
G
F
 
F
R
 
Q
L
 
T
V
 
A
D
 
S
K
 
E
S
 
D
E
 
E
L
 
L
P
 
P

Sites not aligning to the query:

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
33% identity, 83% coverage: 2:124/149 of query aligns to 277:401/424 of 3b8gA

query
sites
3b8gA
L
 
I
R
 
R
K
 
Q
A
 
A
Q
 
H
I
 
S
S
 
G
D
 
D
V
 
I
K
 
P
N
 
H
I
 
I
Q
 
A
K
 
A
L
 
L
V
 
I
M
 
R
A
 
P
Y
 
L
A
 
E
T
 
E
K
 
Q
G
 
G
D
x
I
M
 
L
L
|
L
S
 
H
R
|
R
S
 
S
L
 
R
S
 
E
E
 
Y
L
 
L
Y
 
E
E
 
N
S
 
H
L
 
I
R
 
S
D
 
E
F
 
F
Y
 
S
V
 
I
Y
 
L
E
 
E
E
 
H
E
 
D
G
 
G
K
 
N
L
 
L
L
 
Y
G
 
G
T
 
C
T
 
A
A
 
A
L
 
L
H
 
K
I
 
T
V
 
F
W
 
A
E
 
E
-
 
A
D
 
D
L
 
C
A
 
G
E
 
E
V
 
I
R
x
A
S
x
C
T
x
L
A
 
A
V
|
V
A
 
S
E
 
P
E
 
Q
A
 
A
T
x
Q
R
x
D
R
 
G
G
|
G
I
x
Y
G
|
G
G
x
E
K
 
R
L
 
L
V
 
L
Q
 
A
A
 
H
C
 
I
I
 
I
D
 
D
E
 
K
G
 
A
R
 
R
K
 
G
L
 
I
G
 
G
L
 
I
K
 
S
R
 
R
L
 
L
F
 
F
C
 
A
L
|
L
-
x
S
T
 
T
Y
 
N
R
x
T
P
 
G
D
x
E
F
x
W
F
 
F
S
 
A
S
 
E
L
 
R
G
 
G
F
 
F
R
 
Q
L
 
T
V
 
A
D
 
S
K
 
E
S
 
D
E
 
E
L
 
L
P
 
P

Sites not aligning to the query:

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
33% identity, 83% coverage: 2:124/149 of query aligns to 277:401/424 of 2r8vA

query
sites
2r8vA
L
 
I
R
 
R
K
 
Q
A
 
A
Q
 
H
I
 
S
S
 
G
D
 
D
V
 
I
K
 
P
N
 
H
I
 
I
Q
 
A
K
 
A
L
 
L
V
 
I
M
 
R
A
 
P
Y
 
L
A
 
E
T
 
E
K
 
Q
G
 
G
D
x
I
M
x
L
L
 
L
S
 
H
R
 
R
S
 
S
L
 
R
S
 
E
E
 
Y
L
 
L
Y
 
E
E
 
N
S
 
H
L
 
I
R
 
S
D
 
E
F
 
F
Y
 
S
V
 
I
Y
 
L
E
 
E
E
 
H
E
 
D
G
 
G
K
 
N
L
 
L
L
 
Y
G
 
G
T
 
C
T
 
A
A
 
A
L
 
L
H
 
K
I
 
T
V
 
F
W
 
A
E
 
E
-
 
A
D
 
D
L
 
C
A
 
G
E
 
E
V
 
I
R
 
A
S
 
C
T
x
L
A
 
A
V
|
V
A
 
S
E
 
P
E
 
Q
A
 
A
T
x
Q
R
x
D
R
 
G
G
|
G
I
 
Y
G
|
G
G
x
E
K
 
R
L
 
L
V
 
L
Q
 
A
A
 
H
C
 
I
I
 
I
D
 
D
E
 
K
G
 
A
R
 
R
K
 
G
L
 
I
G
 
G
L
 
I
K
 
S
R
 
R
L
 
L
F
 
F
C
 
A
L
 
L
-
 
S
T
 
T
Y
 
N
R
x
T
P
 
G
D
x
E
F
x
W
F
|
F
S
 
A
S
 
E
L
 
R
G
 
G
F
 
F
R
 
Q
L
 
T
V
 
A
D
 
S
K
 
E
S
 
D
E
 
E
L
 
L
P
 
P

Sites not aligning to the query:

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
32% identity, 83% coverage: 2:124/149 of query aligns to 285:405/424 of 3d2pA

query
sites
3d2pA
L
 
I
R
 
R
K
 
Q
A
 
A
Q
 
H
I
 
S
S
 
G
D
 
D
V
 
I
K
 
P
N
 
H
I
 
I
Q
 
A
K
 
A
L
 
L
V
 
I
M
 
R
A
 
P
Y
x
L
A
 
I
T
 
-
K
 
-
G
 
-
D
 
-
M
 
L
L
 
L
S
 
H
R
 
R
S
 
S
L
 
R
S
 
E
E
 
Y
L
 
L
Y
 
E
E
 
N
S
 
H
L
 
I
R
 
S
D
 
E
F
 
F
Y
 
S
V
 
I
Y
 
L
E
 
E
E
 
H
E
 
D
G
 
G
K
 
N
L
 
L
L
 
Y
G
 
G
T
 
C
T
 
A
A
 
A
L
 
L
H
 
K
I
 
T
V
 
F
W
 
A
E
 
E
-
 
A
D
 
D
L
 
C
A
 
G
E
 
E
V
 
I
R
 
A
S
 
C
T
x
L
A
 
A
V
|
V
A
 
S
E
 
P
E
 
Q
A
 
A
T
x
Q
R
x
D
R
 
G
G
|
G
I
 
Y
G
|
G
G
x
E
K
 
R
L
 
L
V
 
L
Q
 
A
A
 
H
C
 
I
I
 
I
D
 
D
E
 
K
G
 
A
R
 
R
K
 
G
L
 
I
G
 
G
L
 
I
K
 
S
R
 
R
L
 
L
F
 
F
C
 
A
L
 
L
-
 
S
T
 
T
Y
 
N
R
 
T
P
 
G
D
x
E
F
x
W
F
 
F
S
 
A
S
 
E
L
 
R
G
 
G
F
 
F
R
 
Q
L
 
T
V
 
A
D
 
S
K
 
E
S
 
D
E
 
E
L
 
L
P
 
P

Sites not aligning to the query:

P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12) (see paper)
26% identity, 83% coverage: 2:124/149 of query aligns to 298:422/443 of P0A6C5

query
sites
P0A6C5
L
 
I
R
 
R
K
 
R
A
 
A
Q
 
T
I
 
I
S
 
N
D
 
D
V
 
I
K
 
G
N
 
G
I
 
I
Q
 
L
K
 
E
L
 
L
V
 
I
M
 
R
A
 
P
Y
 
L
A
 
E
T
 
Q
K
 
Q
G
 
G
D
 
I
M
 
L
L
 
V
S
 
R
R
 
R
S
 
S
L
 
R
S
 
E
E
 
Q
L
 
L
Y
 
E
E
 
M
S
 
E
L
 
I
R
 
D
D
 
K
F
 
F
Y
 
T
V
 
I
Y
 
I
E
 
Q
E
 
R
E
 
D
G
 
N
K
 
T
L
 
T
L
 
I
G
 
A
T
 
C
T
 
A
A
 
A
L
 
L
H
 
Y
I
 
P
V
 
F
W
 
P
E
 
E
D
 
E
-
 
K
L
 
I
A
 
G
E
 
E
V
 
M
R
 
A
S
 
C
T
 
V
A
 
A
V
 
V
A
 
H
E
 
P
E
 
D
A
 
Y
T
 
R
R
 
S
R
 
S
G
 
S
I
 
R
G
 
G
G
 
E
K
 
V
L
 
L
V
 
L
Q
 
E
A
 
R
C
 
I
I
 
A
D
 
A
E
 
Q
G
 
A
R
 
K
K
 
Q
L
 
S
G
 
G
L
 
L
K
 
S
R
 
K
L
 
L
F
 
F
C
 
V
L
 
L
T
 
T
Y
 
T
R
 
R
P
 
S
-
 
I
D
 
H
F
 
W
F
 
F
S
 
Q
S
 
E
L
 
R
G
 
G
F
 
F
R
 
T
L
 
P
V
 
V
D
 
D
K
 
I
S
 
D
E
 
L
L
 
L
P
 
P

Sites not aligning to the query:

6ao7A Crystal structure of a gnat family acetyltransferase from elizabethkingia anophelis with acetyl-coa bound (see paper)
24% identity, 72% coverage: 28:134/149 of query aligns to 26:143/153 of 6ao7A

query
sites
6ao7A
S
 
S
R
 
K
S
 
E
L
 
L
S
 
I
E
 
D
L
 
Q
Y
 
Y
E
 
L
S
 
T
L
 
S
R
 
G
D
 
E
F
 
L
Y
 
Y
V
 
L
Y
 
A
E
 
K
E
 
Y
E
 
N
G
 
N
K
 
E
L
 
I
L
 
I
G
 
G
T
 
C
T
 
Y
A
 
V
L
 
L
H
 
Y
I
 
-
V
 
P
W
 
W
E
 
D
-
 
F
D
 
E
L
 
T
A
 
T
E
 
E
V
 
I
R
x
K
S
x
N
T
x
I
A
|
A
V
|
V
A
 
A
E
 
E
E
 
K
A
 
F
T
x
Q
R
x
N
R
 
Q
G
|
G
I
 
I
G
|
G
G
 
G
K
 
Q
L
 
L
V
 
L
Q
 
K
A
 
D
C
 
V
I
 
I
D
 
L
E
 
K
G
 
A
R
 
K
K
 
N
L
 
K
G
 
F
L
 
Y
K
 
K
R
 
K
L
 
L
F
 
V
C
 
I
L
x
G
T
 
T
Y
 
G
R
 
N
P
 
S
D
 
S
-
 
T
-
x
G
-
x
Q
-
 
L
-
x
Y
F
x
L
F
 
Y
S
 
Q
S
x
K
L
 
Y
G
 
G
F
 
F
R
 
R
L
 
I
V
 
T
D
 
D
-
 
I
-
 
R
-
 
K
-
 
N
-
 
F
-
 
F
K
 
K
S
 
D
E
 
N
L
 
Y
P
 
P
H
 
E
K
 
P
V
 
I
W
 
W
G
 
E
D
 
N
C
 
G
V
 
I
K
 
E
C
 
C

Sites not aligning to the query:

Query Sequence

>WP_011736503.1 NCBI__GCF_000015045.1:WP_011736503.1
MLRKAQISDVKNIQKLVMAYATKGDMLSRSLSELYESLRDFYVYEEEGKLLGTTALHIVW
EDLAEVRSTAVAEEATRRGIGGKLVQACIDEGRKLGLKRLFCLTYRPDFFSSLGFRLVDK
SELPHKVWGDCVKCPKFPDCDENAMILDL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory