SitesBLAST
Comparing WP_011736893.1 NCBI__GCF_000015045.1:WP_011736893.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
55% identity, 100% coverage: 1:267/267 of query aligns to 1:268/268 of 4ywjA
- active site: H156 (= H155), K160 (= K159)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), R12 (= R12), M13 (= M13), D35 (≠ E34), R36 (= R35), F76 (= F75), T77 (= T76), V81 (= V80), G99 (= G98), T101 (= T100), A124 (≠ P123), N125 (= N124), F126 (≠ M125), R237 (= R236), F240 (= F239)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
51% identity, 100% coverage: 1:267/267 of query aligns to 1:267/269 of 5tejB
- active site: H155 (= H155), K159 (= K159)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T100), H156 (= H156), K159 (= K159), S164 (= S164), G165 (= G165), T166 (= T166)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (= R35), F75 (= F75), T76 (= T76), S80 (≠ V80), G98 (= G98), T100 (= T100), P123 (= P123), N124 (= N124), Y125 (≠ M125), F239 (= F239)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
51% identity, 100% coverage: 1:267/267 of query aligns to 1:267/269 of 5tejA
- active site: H155 (= H155), K159 (= K159)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (= R35), F75 (= F75), T76 (= T76), S80 (≠ V80), G98 (= G98), T100 (= T100), P123 (= P123)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
51% identity, 100% coverage: 1:266/267 of query aligns to 1:266/266 of 5temA
- active site: H155 (= H155), K159 (= K159)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (= R35), F75 (= F75), T76 (= T76), S80 (≠ V80), G98 (= G98), T100 (= T100), P123 (= P123), N124 (= N124), Y125 (≠ M125), F239 (= F239)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T100), P123 (= P123), H156 (= H156), K159 (= K159), S164 (= S164), G165 (= G165), T166 (= T166)
1drvA Escherichia coli dhpr/acnadh complex (see paper)
48% identity, 100% coverage: 2:267/267 of query aligns to 3:268/270 of 1drvA
- active site: H156 (= H155), K160 (= K159)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (= E34), F76 (= F75), T77 (= T76), R78 (≠ A77), G81 (≠ V80), G99 (= G98), A124 (≠ P123), F126 (≠ M125), R237 (= R236)
1druA Escherichia coli dhpr/nadh complex (see paper)
48% identity, 100% coverage: 2:267/267 of query aligns to 3:268/270 of 1druA
- active site: H156 (= H155), K160 (= K159)
- binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (= E34), R36 (= R35), F76 (= F75), T77 (= T76), R78 (≠ A77), G81 (≠ V80), G99 (= G98), T100 (≠ S99), T101 (= T100), A124 (≠ P123), N125 (= N124), F126 (≠ M125), F240 (= F239)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
48% identity, 100% coverage: 2:267/267 of query aligns to 3:268/270 of 1arzA
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
48% identity, 100% coverage: 2:267/267 of query aligns to 2:267/269 of 1arzB
- active site: H155 (= H155), K159 (= K159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G8), G10 (≠ A10), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), F75 (= F75), T76 (= T76), R77 (≠ A77), G80 (≠ V80), H84 (≠ N84), G98 (= G98), T100 (= T100), A123 (≠ P123), N124 (= N124), F125 (≠ M125), F239 (= F239)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T100), H156 (= H156), K159 (= K159), S164 (= S164), G165 (= G165), T166 (= T166), F239 (= F239)
1drwA Escherichia coli dhpr/nhdh complex (see paper)
48% identity, 100% coverage: 2:267/267 of query aligns to 5:270/272 of 1drwA
- active site: H158 (= H155), K162 (= K159)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), E37 (= E34), R38 (= R35), F78 (= F75), T79 (= T76), R80 (≠ A77), G101 (= G98), T102 (≠ S99), T103 (= T100), A126 (≠ P123), N127 (= N124), F128 (≠ M125), F242 (= F239)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
48% identity, 100% coverage: 2:267/267 of query aligns to 5:270/272 of 1dihA
- active site: H158 (= H155), K162 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), R38 (= R35), F78 (= F75), T79 (= T76), R80 (≠ A77), G83 (≠ V80), G101 (= G98), T103 (= T100), N127 (= N124), F128 (≠ M125), R239 (= R236), F242 (= F239)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
48% identity, 100% coverage: 2:267/267 of query aligns to 6:271/273 of P04036
- G12 (= G8) binding NADP(+)
- GRM 15:17 (= GRM 11:13) binding NAD(+)
- RM 16:17 (= RM 12:13) binding NADP(+)
- E38 (= E34) binding NAD(+)
- R39 (= R35) binding NADP(+)
- TR 80:81 (≠ TA 76:77) binding NAD(+)
- GTT 102:104 (≠ GST 98:100) binding NAD(+); binding NADP(+)
- AANF 126:129 (≠ APNM 122:125) binding NAD(+)
- F129 (≠ M125) binding NADP(+)
- H159 (= H155) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K159) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R236) binding NADP(+)
- F243 (= F239) binding NAD(+)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
40% identity, 100% coverage: 2:267/267 of query aligns to 2:267/267 of 3ijpB
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
40% identity, 99% coverage: 2:266/267 of query aligns to 2:266/266 of 3ijpA
- active site: H155 (= H155), K159 (= K159)
- binding sodium ion: I21 (≠ V21), Q22 (= Q22), R24 (≠ A24), V27 (= V27)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), N10 (≠ A10), G11 (= G11), R12 (= R12), M13 (= M13), R35 (= R35), F75 (= F75), S76 (≠ T76), Q77 (≠ A77), A80 (≠ V80), G98 (= G98), T100 (= T100), G123 (≠ P123), N124 (= N124), M125 (= M125), F239 (= F239)
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
32% identity, 99% coverage: 2:264/267 of query aligns to 6:217/218 of 1vm6B
- active site: H132 (= H155), K136 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G8), S14 (≠ A10), G15 (= G11), R16 (= R12), M17 (= M13), D37 (= D61), V38 (≠ L62), F53 (= F75), S54 (≠ T76), S55 (≠ A77), E57 (≠ K79), A58 (≠ V80), G76 (= G98), T78 (= T100), Y101 (≠ P123), N102 (= N124), F103 (≠ M125), F192 (= F239)
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
32% identity, 99% coverage: 2:264/267 of query aligns to 1:212/216 of Q9X1K8
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
29% identity, 99% coverage: 1:264/267 of query aligns to 2:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ A10), G12 (= G11), K13 (≠ R12), M14 (= M13), D35 (≠ E34), H36 (vs. gap), K37 (≠ R35), L76 (≠ F75), T77 (= T76), G99 (= G98), T100 (≠ S99), T101 (= T100), P126 (= P123), N127 (= N124), F128 (≠ M125)
- binding pyridine-2,6-dicarboxylic acid: P126 (= P123), H155 (= H155), H156 (= H156), K159 (= K159), S164 (= S164), G165 (= G165), T166 (= T166), A215 (≠ G214)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
29% identity, 99% coverage: 1:264/267 of query aligns to 2:265/265 of 5z2eA
5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
29% identity, 99% coverage: 2:266/267 of query aligns to 2:247/247 of 5eesA
- active site: H133 (= H155), K137 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G11 (= G11), R12 (= R12), V13 (≠ M13), G34 (= G52), V35 (≠ I58), F53 (= F75), T54 (= T76), G76 (= G98), T78 (= T100), P104 (= P123), N105 (= N124), F106 (≠ M125), F220 (= F239)
- binding sulfate ion: H134 (= H156), K137 (= K159), K137 (= K159), G143 (= G165), T144 (= T166)
5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
29% identity, 99% coverage: 2:266/267 of query aligns to 2:247/247 of 5eerA
1yl5A Crystal structure of mycobacterium tuberculosis dihydrodipicolinate reductase (rv2773c) (crystal form a) (see paper)
31% identity, 96% coverage: 1:257/267 of query aligns to 2:236/247 of 1yl5A
Query Sequence
>WP_011736893.1 NCBI__GCF_000015045.1:WP_011736893.1
MIKIAVCGAAGRMGQRLINSVQEAEGVCLSGVLERPAHPLVGQDAGLVAGCGALGVSISD
DLNAVIAGCDVLIDFTAPKVSLKNLEACGLQRKSIVIGSTGFTPEERQLAMELTRNISAI
LAPNMSVGVNVCFKILKDIAATLGDDFDVEIVESHHRMKVDAPSGTAVRMGQVVAEALGR
DYDRVANFHREGITGSRTKDEIGMQTIRGGDIVGEHTVYFIGMGERIELSHRAMNRDMFS
RGAVRAATWVVGQAPGLYDMQDVLGLK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory