SitesBLAST

W22 (≠ D17) mutation to A: Decreased heat stability.
E26 (≠ A21) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ E25) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ E29) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (≠ N220) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (≠ Q233) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E270) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8qeuA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with ornithine (see paper)
48% identity, 99% coverage: 3:303/305 of query aligns to 2:303/304 of 8qeuA

query
sites
8qeuA

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K

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D

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G

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binding L-ornithine: L125 (= L125), N161 (= N161), D222 (= D222), S226 (= S226), M227 (= M227), L263 (= L263)

P9WIT9 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 99% coverage: 1:302/305 of query aligns to 1:304/307 of P9WIT9

query
sites
P9WIT9

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A

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L

STRT 50:53 (= STRT 52:55) binding carbamoyl phosphate
Q77 (= Q79) binding carbamoyl phosphate
R101 (= R103) binding carbamoyl phosphate
HPCQ 128:131 (= HPCQ 130:133) binding carbamoyl phosphate
N160 (= N161) binding L-ornithine
D224 (= D222) binding L-ornithine
SM 228:229 (= SM 226:227) binding L-ornithine
CL 264:265 (= CL 262:263) binding carbamoyl phosphate
R292 (= R290) binding carbamoyl phosphate

8qevA Crystal structure of ornithine transcarbamylase from arabidopsis thaliana (atotc) in complex with carbamoyl phosphate (see paper)
46% identity, 99% coverage: 3:303/305 of query aligns to 2:296/297 of 8qevA

query
sites
8qevA

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W

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A

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G

-

M

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-

A

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N

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L

G

G

binding phosphoric acid mono(formamide)ester: S52 (= S52), M53 (≠ T53), R54 (= R54), T55 (= T55), R103 (= R103), C255 (= C262), L256 (= L263), R283 (= R290)

7nouA Crystal structure of mycobacterium tuberculosis argf in complex with (3,5-dichlorophenyl)boronic acid.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:305/308 of 7nouA

query
sites
7nouA

R

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A

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L

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R

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A

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K

A

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M

L

active site: R102 (= R103), H129 (= H130), Q132 (= Q133), D225 (= D222), C265 (= C262), R293 (= R290)
binding [3,5-bis(chloranyl)phenyl]-oxidanyl-oxidanylidene-boron: I46 (= I47), T52 (= T53), R53 (= R54), R53 (= R54), F56 (≠ I57), F56 (≠ I57), L79 (≠ M80), D82 (≠ G83), E83 (= E84), V91 (= V92), Y95 (= Y96), L266 (= L263), R293 (= R290)

7nosA Crystal structure of mycobacterium tuberculosis argf in complex with 4-bromo-6-(trifluoromethyl)-1h-benzo[d]imidazole.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:305/308 of 7nosA

query
sites
7nosA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I
|
I

F

F

D

D

K

K

S

N

S

S

T

T

R

R

T

T

R

R

I

F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S

T

Q

Q

M

L

G

G

R

R

G
x
D

E
|
E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L
|
L

S

S

R

R

Y

Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R102 (= R103), H129 (= H130), Q132 (= Q133), D225 (= D222), C265 (= C262), R293 (= R290)
binding 4-bromanyl-6-(trifluoromethyl)-1~{H}-benzimidazole: I46 (= I47), D82 (≠ G83), E83 (= E84), V91 (= V92), L92 (= L93)

7norA Crystal structure of mycobacterium tuberculosis argf in complex with 2-fluoro-4-hydroxybenzonitrile.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:305/308 of 7norA

query
sites
7norA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S

S

T
|
T

R

R

T

T

R

R

I
x
F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S
x
T

Q

Q

M
x
L

G

G

R

R

G

D

E

E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

Y

Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R102 (= R103), H129 (= H130), Q132 (= Q133), D225 (= D222), C265 (= C262), R293 (= R290)
binding 2-fluoro-4-hydroxybenzonitrile: T52 (= T53), F56 (≠ I57), T77 (≠ S78), L79 (≠ M80)

7nnyA Crystal structure of mycobacterium tuberculosis argf in complex with naphthalen-1-ol.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:305/308 of 7nnyA

query
sites
7nnyA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S

S

T
|
T

R
|
R

T

T

R

R

I
x
F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S

T

Q

Q

M

L

G

G

R

R

G

D

E
|
E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

Y
|
Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R102 (= R103), H129 (= H130), Q132 (= Q133), D225 (= D222), C265 (= C262), R293 (= R290)
binding 1-naphthol: T52 (= T53), R53 (= R54), F56 (≠ I57), E83 (= E84), V91 (= V92), Y95 (= Y96)

7nnwA Crystal structure of mycobacterium tuberculosis argf in complex with methyl 4-hydroxy-3-iodobenzoate.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:305/308 of 7nnwA

query
sites
7nnwA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I
|
I

F

F

D

D

K

K

S

N

S

S

T
|
T

R
|
R

T

T

R

R

I
x
F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S

T

Q

Q

M
x
L

G

G

R

R

G

D

E

E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V

V

L
|
L

S

S

R

R

Y
|
Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R102 (= R103), H129 (= H130), Q132 (= Q133), D225 (= D222), C265 (= C262), R293 (= R290)
binding methyl 3-iodanyl-4-oxidanyl-benzoate: I46 (= I47), T52 (= T53), R53 (= R54), F56 (≠ I57), L79 (≠ M80), L92 (= L93), Y95 (= Y96)

7nnvA Crystal structure of mycobacterium tuberculosis argf in complex with carbamoyl phosphate.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:305/308 of 7nnvA

query
sites
7nnvA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

I

F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S

T

Q

Q

M

L

G

G

R

R

G

D

E

E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

Y

Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R102 (= R103), H129 (= H130), Q132 (= Q133), D225 (= D222), C265 (= C262), R293 (= R290)
binding phosphoric acid mono(formamide)ester: S51 (= S52), T52 (= T53), R53 (= R54), T54 (= T55), R102 (= R103), H129 (= H130), C265 (= C262), L266 (= L263), R293 (= R290)

2i6uA Crystal structure of ornithine carbamoyltransferase complexed with carbamoyl phosphate and l-norvaline from mycobacterium tuberculosis (rv1656) at 2.2 a (see paper)
50% identity, 98% coverage: 3:302/305 of query aligns to 3:304/307 of 2i6uA

query
sites
2i6uA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S
|
S

T
|
T

R
|
R

T
|
T

R

R

I

F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S

T

Q

Q

M

L

G

G

R
|
R

G

D

E

E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

Y

Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L
|
L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

N
|
N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S
|
S

M
|
M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R52 (= R54), T53 (= T55), R80 (= R82), R101 (= R103), H128 (= H130), Q131 (= Q133), D224 (= D222), C264 (= C262), R292 (= R290)
binding phosphoric acid mono(formamide)ester: S50 (= S52), T51 (= T53), R52 (= R54), T53 (= T55), R101 (= R103), C264 (= C262), L265 (= L263), R292 (= R290)
binding norvaline: L123 (= L125), N160 (= N161), D224 (= D222), S228 (= S226), M229 (= M227)

7np0A Crystal structure of mycobacterium tuberculosis argf in complex with (4-nitrophenyl)boronic acid.
50% identity, 98% coverage: 3:302/305 of query aligns to 4:302/305 of 7np0A

query
sites
7np0A

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S

S

T

T

R

R

T

T

R

R

I

F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

-

S

-

Q

-

M

L

G

G

R

R

G

D

E
|
E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

Y
|
Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R99 (= R103), H126 (= H130), Q129 (= Q133), D222 (= D222), C262 (= C262), R290 (= R290)
binding p-nitrophenylboronic acid: E80 (= E84), V88 (= V92), Y92 (= Y96)

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
45% identity, 99% coverage: 2:302/305 of query aligns to 11:309/316 of Q81M99

query
sites
Q81M99

A

T

R

K

H

D

F

L

L

L

S

T

L

L

H

E

D

E

Y

L

T

T

K

Q

E

E

L

I

D

I

R

S

L

L

L

I

A

E

L

F

A

A

I

I

E

Y

L

L

K

K

Q

K

E

N

Q

K

K

Q

Q

E

G

P

G

-

E

-

H

-

L

L

K

Q

G

G

K

K

T

I

L

L

A

G

M

L

I

I

F

F

D

D

K

K

S

H

S
|
S

T
|
T

R
|
R

T
|
T

R

R

I

V

S

S

F

F

E

E

V

A

G

G

I

M

Y

V

Q

Q

L

L

G

G

H

H

G

G

L

M

F

F

I

L

S

N

T

G

G

K

T

E

S

M

Q
|
Q

M

M

G

G

R

R

G

G

E

E

P

T

L

V

K

S

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

H

Y

Y

C

I

D

D

G

G

I

I

M

M

I

I

R
|
R

T

T

F

F

G

S

Q

H

E

A

I

D

V

V

D

E

E

E

L

L

A

A

A

K

Y

E

A

S

T

S

V

I

P

P

V

V

I

I

N

N

G

G

L

L

T

T

D

D

L

D

F

H

H
|
H

P
|
P

C
|
C

Q
|
Q

I

A

M

L

A

A

D

D

V

L

M

M

T

T

V

I

M

Y

E

E

H

E

K

T

G

N

G

T

Y

F

A

K

G

G

L

I

K

K

F

L

A

A

W

Y

V

V

G

G

D

D

G

G

N

N

N
|
N

M

V

A

C

N

H

T

S

W

L

I

L

E

L

A

A

A

S

A

A

I

K

F

V

G

G

F

M

D

H

L

M

A

T

L

V

A

A

C

T

P

P

Q

V

G

G

Y

Y

E

K

P

P

D

N

A

E

K

E

V

I

M

V

A

K

W

K

A

A

-

L

-

A

-

I

A

A

E

K

R

E

A

T

P

G

G

A

R

E

V

I

T

E

L

I

T

L

A

H

D

N

P

P

K

E

E

L

A

A

V

V

S

N

G

E

A

A

H

D

V

F

I

I

N

Y

T

T

D
|
D

V

V

W

W

A

M

S
|
S

M
|
M

G

G

M

Q

E

E

A

G

E

E

Q

E

K

E

V

-

R

K

E

Y

Q

T

A

L

F

F

G

Q

G

P

F

Y

C

Q

L

I

D

N

D

K

A

E

L

L

A

V

G

K

L

H

A

A

H

K

P

Q

D

T

H

Y

I

H

V

F

L

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

E

E

E

I

V

S

T

D

G

S

E

V

I

I

I

E

D

G

G

S

P

N

Q

S

S

R

I

V

V

W

F

D

E

E

Q

A

A

E

G

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

S

L

L

M

F

STRT 57:60 (= STRT 52:55) binding carbamoyl phosphate
Q84 (= Q79) binding carbamoyl phosphate
R108 (= R103) binding carbamoyl phosphate
HPCQ 135:138 (= HPCQ 130:133) binding carbamoyl phosphate
N166 (= N161) binding L-ornithine
D230 (= D222) binding L-ornithine
SM 234:235 (= SM 226:227) binding L-ornithine
CL 269:270 (= CL 262:263) binding carbamoyl phosphate
R297 (= R290) binding carbamoyl phosphate

4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
45% identity, 99% coverage: 2:302/305 of query aligns to 7:305/307 of 4nf2A

query
sites
4nf2A

A

T

R

K

H

D

F

L

L

L

S

T

L

L

H

E

D

E

Y

L

T

T

K

Q

E

E

L

I

D

I

R

S

L

L

L

I

A

E

L

F

A

A

I

I

E

Y

L

L

K

K

Q

K

E

N

Q

K

K

Q

Q

E

G

P

G

-

E

-

H

-

L

L

K

Q

G

G

K

K

T

I

L

L

A

G

M

L

I

I

F

F

D

D

K

K

S

H

S
|
S

T
|
T

R
|
R

T
|
T

R

R

I

V

S

S

F

F

E

E

V

A

G

G

I

M

Y

V

Q

Q

L

L

G

G

H

H

G

G

L

M

F

F

I

L

S

N

T

G

G

K

T

E

S

M

Q

Q

M

M

G

Q

R
|
R

G

G

E

E

P

T

L

V

K

S

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

H

Y

Y

C

I

D

D

G

G

I

I

M

M

I

I

R
|
R

T

T

F

F

G

S

Q

H

E

A

I

D

V

V

D

E

E

E

L

L

A

A

A

K

Y

E

A

S

T

S

V

I

P

P

V

V

I

I

N

N

G

G

L
|
L

T

T

D

D

L

D

F

H

H
|
H

P

P

C

C

Q
|
Q

I

A

M

L

A

A

D

D

V

L

M

M

T

T

V

I

M

Y

E

E

H

E

K

T

G

N

G

T

Y

F

A

K

G

G

L

I

K

K

F

L

A

A

W

Y

V

V

G

G

D

D

G

G

N

N

N
|
N

M

V

A

C

N

H

T

S

W

L

I

L

E

L

A

A

A

S

A

A

I

K

F

V

G

G

F

M

D

H

L

M

A

T

L

V

A

A

C

T

P

P

Q

V

G

G

Y

Y

E

R

P

P

D

N

A

E

K

E

V

I

M

V

A

K

W

K

A

A

-

L

-

A

-

I

A

A

E

K

R

E

A

T

P

G

G

A

R

E

V

I

T

E

L

I

T

L

A

H

D

N

P

P

K

E

E

L

A

A

V

V

S

N

G

E

A

A

H

D

V

F

I

I

N

Y

T

T

D
|
D

V

V

W

W

A

M

S
|
S

M
|
M

G

G

M

Q

E

E

A

G

E

E

Q

E

K

E

V

-

R

K

E

Y

Q

T

A

L

F

F

G

Q

G

P

F

Y

C

Q

L

I

D

N

D

K

A

E

L

L

A

V

G

K

L

H

A

A

H

K

P

Q

D

T

H

Y

I

H

V

F

L

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

E

E

E

I

V

S

T

D

G

S

E

V

I

I

I

E

D

G

G

S

P

N

Q

S

S

R

I

V

V

W

F

D

E

E

Q

A

A

E

G

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

S

L

L

M

F

active site: R55 (= R54), T56 (= T55), R83 (= R82), R104 (= R103), H131 (= H130), Q134 (= Q133), D226 (= D222), C265 (= C262), R293 (= R290)
binding phosphoric acid mono(formamide)ester: S53 (= S52), T54 (= T53), R55 (= R54), T56 (= T55), R104 (= R103), H131 (= H130), Q134 (= Q133), C265 (= C262), L266 (= L263), R293 (= R290)
binding norvaline: L126 (= L125), N162 (= N161), D226 (= D222), S230 (= S226), M231 (= M227)

7nnzB Crystal structure of mycobacterium tuberculosis argf in complex with 5-methyl-4-phenylthiazol-2-amine.
49% identity, 98% coverage: 3:302/305 of query aligns to 3:294/297 of 7nnzB

query
sites
7nnzB

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S

S

T
|
T

R
|
R

T

T

R

R

I
x
F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

G

T

S

S

T

Q

Q

M

L

G

G

R

R

G

D

E

E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V

V

L

L

S

S

R

R

Y

Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

-

S

-

M

-

G

-

M

-

E

-

A

-

E

-

Q

-

K

-

V

T

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L

L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A

A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R101 (= R103), H128 (= H130), Q131 (= Q133), D224 (= D222), C254 (= C262), R282 (= R290)
binding 5-methyl-4-phenyl-1,3-thiazol-2-amine: T51 (= T53), R52 (= R54), F55 (≠ I57)

7novA Crystal structure of mycobacterium tuberculosis argf in complex with (4-methyl-3-nitrophenyl)boronic acid.
49% identity, 98% coverage: 3:302/305 of query aligns to 4:299/302 of 7novA

query
sites
7novA

R

R

H

H

F

F

L

L

S

R

L

D

H

D

D

D

Y

L

T

S

K

P

E

A

E

E

L

Q

D

A

R

E

L

V

L

L

A

E

L

L

A

A

I

A

E

E

L

L

K

K

Q

K

E

D

Q

P

K

V

Q

-

G

-

G

S

E

R

H

R

H

P

L

L

L

Q

K

G

G

P

K

R

T

G

L

V

A

A

M

V

I

I

F

F

D

D

K

K

S

N

S

S

T

T

R
|
R

T

T

R

R

I
x
F

S

S

F

F

E

E

V

L

G

G

I

I

Y

A

Q

Q

L

L

G

G

H

H

G

A

L

V

F

V

I

V

S

D

T

S

G

-

T

-

S

-

Q

-

M

-

G

-

R

R

G

D

E
|
E

P

T

L

L

K

Q

D

D

T

T

A

A

R

K

V
|
V

L

L

S

S

R

R

Y
|
Y

C

V

D

D

G

A

I

I

M

V

I

W

R
|
R

T

T

F

F

G

G

Q

Q

E

E

I

R

V

L

D

D

E

A

L

M

A

A

A

S

Y

V

A

A

T

T

V

V

P

P

V

V

I

I

N

N

G

A

L

L

T

S

D

D

L

E

F

F

H
|
H

P

P

C

C

Q
|
Q

I

V

M

L

A

A

D

D

V

L

M

Q

T

T

V

I

M

A

E

E

H

R

K

K

G

G

G

A

Y

L

A

R

G

G

L

L

K

R

F

L

A

S

W

Y

V

F

G

G

D

D

G

G

-

A

N

N

M

M

A

A

N

H

T

S

W

L

I

L

E

L

A

G

A

V

I

T

F

A

G

G

F

I

D

H

L

V

A

T

L

V

A

A

C

A

P

P

Q

E

G

G

Y

F

E

L

P

P

D

D

A

P

K

S

V

V

M

R

A

A

W

A

A

A

A

E

E

R

R

R

A

A

P

Q

-

D

-

T

-

G

G

A

R

S

V

V

T

T

L

V

T

T

A

A

D

D

P

A

K

H

E

A

A

A

V

A

S

A

G

G

A

A

H

D

V

V

I

L

N

V

T

T

D
|
D

V

T

W

W

A

T

S

S

M

M

G

G

M

Q

E

E

A

N

E

D

Q

G

K

L

V

D

R

R

E

V

Q

K

A

P

F

F

G

R

G

P

F

F

C

Q

L

L

D

N

D

S

A

R

L

L

A

L

G

A

L

L

A

A

H

D

P

S

D

D

H

A

I

I

V

V

L

L

H

H

C
|
C

L
|
L

P

P

A

A

H

H

R

R

G

G

E

D

E

E

I

I

S

T

D

D

S

A

V

V

I

M

E

D

G

G

S

P

N

A

S

S

R

A

V

V

W

W

D

D

E

E

A
|
A

E

E

N

N

R
|
R

L

L

H

H

A

A

Q

Q

K

K

A

A

I

L

M

L

A

V

T

W

L

L

M

L

active site: R96 (= R103), H123 (= H130), Q126 (= Q133), D219 (= D222), C259 (= C262), R287 (= R290)
binding (4-methyl-3-nitro-phenyl)-oxidanyl-oxidanylidene-boron: R53 (= R54), F56 (≠ I57), E77 (= E84), V85 (= V92), Y89 (= Y96), L260 (= L263), A284 (= A287), R287 (= R290)

P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
45% identity, 98% coverage: 3:302/305 of query aligns to 40:342/354 of P00481

query
sites
P00481

R

R

H

D

F

L

L

L

S

T

L

L

H

K

D

N

Y

F

T

T

K

G

E

E

L

I

D

Q

R

Y

L

M

L

L

A

W

L

L

A

S

I

A

E

D

L

L

K

K

Q

F

E

R

Q

I

K

K

Q

Q

G

K

G

G

E

E

H

Y

-

L

H

P

L

L

K

Q

G

G

K

K

T

S

L

L

A

G

M

M

I

I

F

F

D

E

K

K

S

R

S

S

T

T

R
|
R

T

T

R

R

I

L

S

S

F

T

E

E

V

T

G

G

I

F

Y

A

Q

L

L

L

G

G

H

H

G

P

L

S

F

F

I

L

S

T

T

T

G

Q

T

D

S

I

Q

H

M

L

G

G

R

V

G

N

E

E

P

S

L

L

K

T

D

D

T

T

A

A

R

R

V

V

L

L

S

S

R

S

Y

M

C

T

D

D

G

A

I

V

M

L

I

A

R

R

T

V

F

Y

G

K

Q

Q

E

S

I

D

V

L

D

D

E

I

L

L

A

A

A

K

Y

E

A

A

T

T

V

I

P

P

V

I

I

V

N

N

G

G

L

L

T

S

D

D

L

L

F

Y

H

H

P

P

C

I

Q

Q

I

I

M

L

A

A

D

D

V

Y

M

L

T

T

V

L

M

Q

E

E

H

H

K

Y

G

G

G

S

Y

L

A

K

G

G

L

L

K

T

F

L

A

S

W

W

V

I

G

G

D

D

G

G

N

N

M

I

A

L

N

H

T

S

W

I

I

M

E

M

A

S

A

A

I

K

F

F

G

G

F

M

D

H

L

L

A

Q

L

A

A

A

C

T

P

P

Q

K

G

G

Y

Y

E

E

P

P

D

D

A

P

K

N

V

I

M

V

A

K

W

L

A

A

A

E

E

Q

R

Y

A

A

P

K

-

E

-

N

-

G

G

T

R

R

V

L

T

S

L

M

T

T

A

N

D

D

P

P

K

L

E

E

A

A

V

A

S

R

G

G

A

G

H

N

V

V

I

L

N

I

T

T

D

D

V

T

W

W

A

I

S

S

M

M

G

G

M

Q

E

E

A

D

E

E

Q

K

K

K

V

K

R

R

E

L

Q

Q

A

A

F

F

G

Q

G

G

F

Y

C

Q

L

V

D

T

D

M

A

K

L

T

A

A

G

K

L

V

A

A

H

A

P

S

D

D

H

W

I

T

V

F

L

L

H

H

C
|
C

L

L

P

P

A

-

H

R

R

K

G

P

E

E

I

V

S

D

D

D

S

E

V

V

I

F

E

Y

G

S

S

P

N

R

S

S

R

L

V

V

W

F

D

P

E

E

A

A

E

E

N

N

R

R

L

K

H

W

A

T

Q

I

K

M

A

A

I

V

M

M

A

V

T

S

L

L

M

L

R92 (= R54) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
C303 (= C262) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
45% identity, 98% coverage: 3:302/305 of query aligns to 40:342/354 of P00480

query
sites
P00480

R
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L

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-

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L

R40 (= R3) to H: in OTCD; late onset; dbSNP:rs72554308
L43 (= L6) to F: in dbSNP:rs72554309
K46 (≠ H9) to R: in dbSNP:rs1800321
Y55 (≠ R18) to D: in OTCD; late onset; dbSNP:rs72554319
L63 (= L26) to P: in OTCD; late onset; dbSNP:rs72554324
K88 (= K50) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
STRT 90:93 (= STRT 52:55) binding carbamoyl phosphate
G100 (= G62) to D: in OTCD; late onset; dbSNP:rs72554349
F101 (≠ I63) to L: in dbSNP:rs1133135
L111 (≠ I73) to P: in dbSNP:rs1800324
T125 (≠ K87) to M: in OTCD; neonatal; dbSNP:rs72554356
D126 (= D88) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
R129 (= R91) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
A140 (≠ I102) to P: in OTCD; late onset; dbSNP:rs72556260
R141 (= R103) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
H168 (= H130) binding carbamoyl phosphate
Q171 (= Q133) binding carbamoyl phosphate
I172 (= I134) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
Y176 (≠ V138) to C: in OTCD; late onset; dbSNP:rs72556283
TL 178:179 (≠ TV 140:141) natural variant: Missing (in OTCD; neonatal)
Y183 (≠ K145) to D: in OTCD; late onset; dbSNP:rs72556292
G188 (= G150) to R: in OTCD; neonatal; dbSNP:rs72556294
G195 (= G157) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
D196 (= D158) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
L201 (≠ A163) to P: in OTCD; neonatal; dbSNP:rs72558407
S207 (≠ A169) to R: in OTCD; neonatal; dbSNP:rs72558415
A209 (= A171) to V: in OTCD; neonatal; dbSNP:rs72558417
M213 (≠ F175) to K: in OTCD; late onset
H214 (≠ D176) to Y: in OTCD; neonatal; dbSNP:rs72558420
P220 (= P182) to A: in OTCD; late onset; dbSNP:rs72558425
P225 (= P187) to T: in OTCD; late onset; dbSNP:rs72558428
L244 (≠ V203) to Q: in OTCD; late onset; dbSNP:rs72558436
T262 (= T221) to K: in OTCD; mild; dbSNP:rs67333670
T264 (≠ V223) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
W265 (= W224) to L: in OTCD; mild; dbSNP:rs72558446
G269 (= G228) to E: in OTCD; neonatal; dbSNP:rs72558450
Q270 (≠ M229) to R: in dbSNP:rs1800328
E272 (≠ A231) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
R277 (= R236) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
H302 (= H261) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
C303 (= C262) to R: in OTCD; neonatal; dbSNP:rs67468335
CL 303:304 (= CL 262:263) binding carbamoyl phosphate
E309 (= E269) natural variant: Missing (in OTCD; late onset)
R330 (= R290) binding carbamoyl phosphate
T333 (≠ A293) natural variant: T -> A
S340 (≠ T300) to P: in OTCD; late onset; dbSNP:rs72558489

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.
39 G → C: in OTCD; late onset; dbSNP:rs72554306
343 T → K: in OTCD; late onset; dbSNP:rs72558491

1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
45% identity, 98% coverage: 3:302/305 of query aligns to 7:309/321 of 1othA

query
sites
1othA

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-

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active site: R59 (= R54), T60 (= T55), V87 (≠ R82), R108 (= R103), H135 (= H130), Q138 (= Q133), D230 (= D222), C270 (= C262), R297 (= R290)
binding n-(phosphonoacetyl)-l-ornithine: S57 (= S52), T58 (= T53), R59 (= R54), T60 (= T55), R108 (= R103), L130 (= L125), H135 (= H130), N166 (= N161), D230 (= D222), S234 (= S226), M235 (= M227), C270 (= C262), L271 (= L263), R297 (= R290)

1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
45% identity, 98% coverage: 3:302/305 of query aligns to 7:309/321 of 1c9yA

query
sites
1c9yA

R

R

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A

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G

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D

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A

A

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-

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-

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-

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C

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L

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R

K

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Y

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R

S

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A

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M

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active site: R59 (= R54), T60 (= T55), V87 (≠ R82), R108 (= R103), H135 (= H130), Q138 (= Q133), D230 (= D222), C270 (= C262), R297 (= R290)
binding phosphoric acid mono(formamide)ester: S57 (= S52), T58 (= T53), R59 (= R54), T60 (= T55), R108 (= R103), C270 (= C262), L271 (= L263), R297 (= R290)
binding norvaline: L130 (= L125), N166 (= N161), D230 (= D222), S234 (= S226), M235 (= M227)

Query Sequence

>WP_011736997.1 NCBI__GCF_000015045.1:WP_011736997.1
MARHFLSLHDYTKEELDRLLALAIELKQEQKQGGEHHLLKGKTLAMIFDKSSTRTRISFE
VGIYQLGGHGLFISTGTSQMGRGEPLKDTARVLSRYCDGIMIRTFGQEIVDELAAYATVP
VINGLTDLFHPCQIMADVMTVMEHKGGYAGLKFAWVGDGNNMANTWIEAAAIFGFDLALA
CPQGYEPDAKVMAWAAERAPGRVTLTADPKEAVSGAHVINTDVWASMGMEAEQKVREQAF
GGFCLDDALAGLAHPDHIVLHCLPAHRGEEISDSVIEGSNSRVWDEAENRLHAQKAIMAT
LMGGE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory