SitesBLAST
Comparing WP_011737292.1 NCBI__GCF_000015045.1:WP_011737292.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
52% identity, 99% coverage: 4:466/469 of query aligns to 3:464/471 of P04805
- C98 (= C99) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C101) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C126) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ D128) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ T130) mutation to Q: No change in activity or in zinc content.
- H131 (≠ Q132) mutation to Q: No change in activity or in zinc content.
- H132 (≠ P133) mutation to Q: No change in activity or in zinc content.
- C138 (≠ F138) mutation to S: No change in activity or in zinc content.
- S239 (= S240) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
52% identity, 99% coverage: 4:469/469 of query aligns to 3:467/468 of 8i9iA
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
51% identity, 78% coverage: 4:370/469 of query aligns to 3:356/380 of 4g6zA
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
41% identity, 94% coverage: 4:443/469 of query aligns to 2:456/484 of 2cfoA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
41% identity, 94% coverage: 4:443/469 of query aligns to 3:457/485 of Q8DLI5
- R6 (= R7) binding L-glutamate
- Y192 (= Y182) binding L-glutamate
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
38% identity, 99% coverage: 4:468/469 of query aligns to 103:564/564 of 3al0C
- active site: S110 (= S11), K335 (= K241)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (= T23), E142 (= E43), Y276 (= Y182), R294 (= R200), G295 (= G201), D297 (= D203), H298 (= H204), L324 (≠ M230), I325 (= I231), L333 (= L239)
- binding : T144 (= T45), D145 (= D46), R148 (= R49), Y208 (≠ A103), P213 (≠ A108), K252 (= K158), M255 (≠ I161), I266 (= I172), K269 (≠ R175), S270 (= S176), Y276 (= Y182), D297 (= D203), H298 (= H204), L299 (≠ V205), S300 (≠ N206), N301 (= N207), K304 (≠ R210), R330 (≠ K236), P332 (≠ R238), G363 (= G269), W364 (= W270), R365 (≠ S271), E370 (= E276), S387 (≠ G293), K389 (≠ S295), V391 (≠ S297), I392 (≠ V298), K397 (= K303), W400 (= W306), R407 (≠ K313), E446 (= E351), K447 (≠ R352), Q453 (≠ E358), I457 (≠ S362), R509 (≠ K413), K520 (≠ Q425), Q524 (= Q428), R527 (= R431), V535 (≠ H439), T536 (≠ A440), G538 (= G442), L539 (≠ I443)
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
33% identity, 99% coverage: 5:466/469 of query aligns to 3:482/485 of 4griB
- active site: S9 (= S11), K253 (= K241)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y194 (= Y182), R212 (= R200), W216 (≠ H204)
- binding zinc ion: C105 (= C99), C107 (= C101), Y128 (= Y122), C132 (= C126)
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of 1g59A
- binding : D44 (= D46), R45 (≠ V47), A46 (≠ E48), R47 (= R49), P109 (≠ A103), V145 (= V139), R163 (≠ K158), V166 (≠ I161), E172 (= E167), V177 (≠ I172), K180 (≠ R175), S181 (= S176), D182 (= D177), E207 (≠ D202), E208 (≠ D203), R237 (≠ L232), K241 (= K236), T242 (≠ A237), K243 (≠ R238), M273 (≠ L268), G274 (= G269), E282 (= E276), S299 (≠ G293), L300 (≠ R294), P303 (≠ S297), V304 (= V298), K309 (= K303), W312 (= W306), R319 (≠ K313), P357 (≠ E351), R358 (= R352), R417 (≠ K413), K426 (= K422), L427 (≠ M423), Q432 (= Q428), R435 (= R431), L442 (≠ T438), E443 (≠ H439), T444 (≠ A440), P445 (= P441), G446 (= G442), L447 (≠ I443), F448 (≠ G444)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of 2cv2A
- active site: K246 (= K241)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (= S11), G17 (= G19), I21 (≠ T23), E41 (= E43), Y187 (= Y182), R205 (= R200), A206 (≠ G201), E208 (≠ D203), W209 (≠ H204), L235 (≠ M230), L236 (≠ I231)
- binding : S9 (= S11), T43 (= T45), D44 (= D46), R47 (= R49), V145 (= V139), R163 (≠ K158), Y168 (≠ F163), E172 (= E167), V177 (≠ I172), K180 (≠ R175), S181 (= S176), Y187 (= Y182), E207 (≠ D202), E208 (≠ D203), W209 (≠ H204), V211 (≠ N206), R237 (≠ L232), K241 (= K236), L272 (≠ R267), M273 (≠ L268), G274 (= G269), E282 (= E276), S299 (≠ G293), P303 (≠ S297), V304 (= V298), K309 (= K303), W312 (= W306), R319 (≠ K313), P357 (≠ E351), R358 (= R352), R417 (≠ K413), Q432 (= Q428), R435 (= R431), L442 (≠ T438), E443 (≠ H439), T444 (≠ A440), G446 (= G442), L447 (≠ I443), F448 (≠ G444)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of 2cv1A
- active site: K246 (= K241)
- binding adenosine-5'-triphosphate: P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ G20), I21 (≠ T23), R47 (= R49), A206 (≠ G201), W209 (≠ H204), L235 (≠ M230), L236 (≠ I231)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E43), Y187 (= Y182), R205 (= R200), W209 (≠ H204)
- binding : S9 (= S11), E41 (= E43), T43 (= T45), D44 (= D46), R47 (= R49), V145 (= V139), R163 (≠ K158), V166 (≠ I161), E172 (= E167), V177 (≠ I172), K180 (≠ R175), S181 (= S176), Y187 (= Y182), E207 (≠ D202), E208 (≠ D203), W209 (≠ H204), V211 (≠ N206), R237 (≠ L232), K241 (= K236), K243 (≠ R238), M273 (≠ L268), G274 (= G269), S276 (= S271), E282 (= E276), S299 (≠ G293), P303 (≠ S297), V304 (= V298), K309 (= K303), W312 (= W306), R319 (≠ K313), P357 (≠ E351), R358 (= R352), R417 (≠ K413), L427 (≠ M423), Q432 (= Q428), R435 (= R431), L442 (≠ T438), E443 (≠ H439), T444 (≠ A440), G446 (= G442), L447 (≠ I443), F448 (≠ G444)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of 1n78A
- active site: K246 (= K241)
- binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ G20), I21 (≠ T23), E41 (= E43), Y187 (= Y182), N191 (≠ V186), R205 (= R200), A206 (≠ G201), E208 (≠ D203), W209 (≠ H204), L235 (≠ M230), L236 (≠ I231)
- binding : S9 (= S11), T43 (= T45), D44 (= D46), R47 (= R49), V145 (= V139), R163 (≠ K158), V166 (≠ I161), Y168 (≠ F163), E172 (= E167), V177 (≠ I172), K180 (≠ R175), S181 (= S176), Y187 (= Y182), E207 (≠ D202), E208 (≠ D203), W209 (≠ H204), L210 (≠ V205), V211 (≠ N206), R237 (≠ L232), K241 (= K236), M273 (≠ L268), G274 (= G269), E282 (= E276), R297 (≠ N291), P303 (≠ S297), V304 (= V298), K309 (= K303), W312 (= W306), R319 (≠ K313), P357 (≠ E351), R358 (= R352), R417 (≠ K413), L427 (≠ M423), Q432 (= Q428), R435 (= R431), L442 (≠ T438), E443 (≠ H439), T444 (≠ A440), G446 (= G442), L447 (≠ I443), F448 (≠ G444)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of 1j09A
- active site: K246 (= K241)
- binding adenosine-5'-triphosphate: H15 (= H17), E208 (≠ D203), L235 (≠ M230), L236 (≠ I231), K243 (≠ R238), I244 (≠ L239), S245 (= S240), K246 (= K241), R247 (= R242)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y187 (= Y182), N191 (≠ V186), R205 (= R200), W209 (≠ H204)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
38% identity, 97% coverage: 6:462/469 of query aligns to 4:466/468 of P27000
- R358 (= R352) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
34% identity, 98% coverage: 4:464/469 of query aligns to 3:499/502 of 6brlA
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
33% identity, 99% coverage: 1:466/469 of query aligns to 1:469/488 of 8vc5A
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
35% identity, 52% coverage: 7:248/469 of query aligns to 19:248/308 of P27305
- E55 (= E43) binding L-glutamate
- Y182 (= Y182) binding L-glutamate
- R200 (= R200) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
36% identity, 50% coverage: 7:242/469 of query aligns to 7:230/290 of 4a91A
- active site: S11 (= S11), K229 (= K241)
- binding glutamic acid: R7 (= R7), A9 (= A9), S11 (= S11), E43 (= E43), Y170 (= Y182), R188 (= R200), L192 (≠ H204)
- binding zinc ion: C99 (= C99), C101 (= C101), Y113 (= Y122), C117 (= C126)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
33% identity, 57% coverage: 2:269/469 of query aligns to 9:278/455 of 3aiiA
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 46% coverage: 1:218/469 of query aligns to 199:418/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
Query Sequence
>WP_011737292.1 NCBI__GCF_000015045.1:WP_011737292.1
MSDIRVRFAPSPTGYLHVGGARTALFNWLLARKQGGTFILRIEDTDVERSTQESVDAILQ
GMEWLGLDWDEGPFYQTDNFPLYREYIQKLLDEGKAYRCYCTAEELEAKRELAMKEGRKP
KYDGTCRDLTEQPADRPFVVRFRAPQDGGATSFNDLIKGTITFPNDELDDLIVQRSDGTP
TYNFCVVIDDAIMKITTVIRGDDHVNNTPRQVQLYQALGFPVPQFAHVPMILGSDKARLS
KRHGATSVIAYRDMGFLPEALMNYLVRLGWSHGDDEIFSRDEMVAKFDISNVGRSPSVFN
PDKLLWLNAHYIKHGDPQRLADLLVPFLKMRGVDPANGGPQLAVAIKTLQERARTMLEMA
DSALFYYHAPESYDRTALAKFEKEHLLAVYATVAEKLSAATAVTAAEFDALFKEICAEKG
WKMPQVGQPVRIALSGGTHAPGIGEIIVTLGKEETIQRIQRAREFVEKQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory