SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011763917.1 NCBI__GCF_000061505.1:WP_011763917.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
36% identity, 92% coverage: 14:275/285 of query aligns to 14:274/283 of P19938

query
sites
P19938

D

E

E

E

A

V

R

K

V

I

S

D

P

K

M

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

A

V

Y
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Y

E

E

V

V

P

K

V

V

Y

Y

S

N

R

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E

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A

N

E

E

H

H

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R

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Y

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A

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A

A

E

A

K

M

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L

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I

P

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H

Y

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D

K

E

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K

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F

A

H

D

Q

R

L

V

L

T

H

T

E

L

L

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V

A

E

G

K

N

N

P

E

W

L

E

N

D

T

Q

G

S

H

I

I

Y

Y

L

F

Q

Q

V

V

T

T

R

R

G

G

A

T

D

S

A

P

V

-

R
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R

N

A

H
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H

A

Q

F

F

P

P

K

E

D

N

-

T

V

V

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K

P

P

T

V

V

I

F

I

M

G

L

Y

S

T

E

K

P

E

L

-

T

N

P

P

A

R

P

P

-

L

E

E

L

N

L

L

A

E

S

K

G

G

V

V

A

K

A

A

V

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S

F

A

V

A

E

D

D

F

I

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R

W

W

L

L

R

R

C

C

D

D

L

I

K
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K

S

S

V

L

S

N

L

L

A

G

N

A

C

V

L

L

L

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Q

Q

Y

E

G

A

F

H

D

E

Q

K

G

G

C

C

A

Y

E

E

T

A

V

I

L

L

F

H

R

R

D

N

G

N

F

T

L

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T

T

E
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E

G

G

S

S

A

S

S

S

N

N

I

V

F

F

V

G

V

I

R

K

D

D

G

G

R

I

L

L

L

Y

A

T

P

H

P

P

K

A

S

N

H

N

L

M

M

I

L
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L

A

K

G

G

I

I

T

T

Y

R

D

D

V

V

L

I

E

A

L

C

A

A

A

N

A

E

H

I

G

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M

P

P

L

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E

K

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L

I

D

D

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D

G

G

A

K

P

V

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Q

E

Y32 (= Y32) binding substrate
R51 (= R51) binding pyridoxal 5'-phosphate
R99 (= R100) binding substrate
H101 (= H102) binding substrate
K146 (= K147) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
E178 (= E179) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
L202 (= L203) mutation to A: Inactivates enzyme.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
36% identity, 92% coverage: 14:275/285 of query aligns to 13:273/277 of 3daaA

query
sites
3daaA

D

E

E

E

A

V

R

K

V

I

S

D

P

K

M

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

A

V

Y
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Y

E

E

V
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V

V

V

P

K

V

V

Y

Y

S

N

R

G

R

E

P

M

F

F

R

T

L

V

A

N

E

E

H

H

L

I

G

D

R
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R

L

L

A

Y

R

A

T

S

L

A

A

E

A

K

M

I

R

R

L

I

P

T

N

I

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H

Y

S

T

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K

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D

E

K

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F

A

H

D

Q

R

L

V

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H

T

E

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L

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V

A

E

G

K

N

N

P

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Y

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Q

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S

A

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-

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-

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T

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K

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E

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-

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N

P

P

A

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P

P

-

L

E

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L

N

L

L

A

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S

K

G

G

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A

K

A

A

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S

F

A

V

A

E

D

D

F

I

R

R

W

W

L

L

R

R

C

C

D

D

L

I

K
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K

S

S

V

L

S

N

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L

A

G

N

A

C

V

L

L

L

A

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K

Q

Q

Y

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A

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K

G

G

C

C

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Y

E

E

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R

D

N

G

N

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L

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T

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E
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E

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G

S

S

A
x
S

S
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S

N

N

I

V

F

F

V

G

V

I

R

K

D

D

G

G

R

I

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A

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A

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L

A

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G
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G

I
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I

T
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Y

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D

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C

A

A

A

N

A

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K

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K

A

M

D

D

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S

S
x
T

T

T

K

S

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E

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E

L

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D

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Q

E

active site: Y31 (= Y32), V33 (= V34), K145 (= K147), E177 (= E179), L201 (= L203)
binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (= Y32), R50 (= R51), K145 (= K147), E177 (= E179), S180 (≠ A182), S181 (= S183), L201 (= L203), G203 (= G205), I204 (= I206), T205 (= T207), S240 (= S242), T241 (≠ S243)

2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
36% identity, 92% coverage: 14:275/285 of query aligns to 13:273/277 of 2daaA

query
sites
2daaA

D

E

E

E

A

V

R

K

V

I

S

D

P

K

M

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

A

V

Y
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Y

E

E

V
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V

V

V

P

K

V

V

Y

Y

S

N

R

G

R

E

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M

F

F

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A

N

E

E

H

H

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R
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R

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L

A

Y

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A

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L

A

A

E

A

K

M

I

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I

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K

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F

A

H

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Q

R

L

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L

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H

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E

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L

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V

A

E

G

K

N

N

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E

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Y

L

F

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Q

V

V

T

T

R

R

G

G

A

T

D

S

A

P

V

-

R
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R

N

A

H
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H

A

Q

F

F

P

P

K

E

D

N

-

T

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K

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V

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G

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K

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E

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N

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P

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L

E

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A

A

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D

F

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W

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L

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R

C

C

D

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K

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L

L

A

G

N

A

C

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L

L

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C

A

Y

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H

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R

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G

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E
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E

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G

S

S

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x
S

S
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S

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N

I

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F

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K

D

D

G

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A

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N

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M

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L
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L

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G

I
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I

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Y

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D

D

V

V

L

I

E

A

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C

A

A

A

N

A

E

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K

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K

A

M

D

D

E

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L

L

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F

M

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S
x
T

T

T

K

S

E

E

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T

A

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E

L

I

D

D

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G

A

K

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K

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Q

E

active site: Y31 (= Y32), V33 (= V34), K145 (= K147), E177 (= E179), L201 (= L203)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (= Y32), V33 (= V34), R50 (= R51), R98 (= R100), H100 (= H102), K145 (= K147), E177 (= E179), S180 (≠ A182), S181 (= S183), N182 (= N184), L201 (= L203), G203 (= G205), I204 (= I206), T205 (= T207), T241 (≠ S243)

1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
36% identity, 92% coverage: 14:275/285 of query aligns to 13:273/277 of 1daaA

query
sites
1daaA

D

E

E

E

A

V

R

K

V

I

S

D

P

K

M

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

A

V

Y
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Y

E

E

V
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V

V

V

P

K

V

V

Y

Y

S

N

R

G

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M

F

F

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A

N

E

E

H

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L

A

A

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A

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H

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N

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Y

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F

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Q

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V

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T

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R

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G

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S

A

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-

R

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N

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H

H

A

Q

F

F

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K

E

D

N

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T

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V

I

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P

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L

E

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L

L

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G

G

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K

A

A

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F

A

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A

E

D

D

F

I

R

R

W

W

L

L

R

R

C

C

D

D

L

I

K
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K

S

S

V

L

S

N

L

L

A

G

N

A

C

V

L

L

L

A

R

K

Q

Q

Y

E

G

A

F

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D

E

Q

K

G

G

C

C

A

Y

E

E

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A

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L

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H

R

R

D

N

G

N

F

T

L

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T

T

E
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E

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G

S

S

A
x
S

S
|
S

N

N

I

V

F

F

V

G

V

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R

K

D

D

G

G

R

I

L

L

L

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A

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P

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A

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N

H

N

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M

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L
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L

A

K

G
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G

I
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I

T
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T

Y

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D

D

V

V

L

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E

A

L

C

A

A

A

N

A

E

H

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P

L

V

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K

V

E

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I

E

P

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D

T

A

H

E

E

V

A

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K

A

M

D

D

E

E

L

L

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T

S
|
S

S
x
T

T

T

K

S

E

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A

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I

V

T

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R

E

L

I

D

D

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Q

A

K

W

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Q

E

active site: Y31 (= Y32), V33 (= V34), K145 (= K147), E177 (= E179), L201 (= L203)
binding pyridoxal-5'-phosphate: R50 (= R51), K145 (= K147), E177 (= E179), S180 (≠ A182), S181 (= S183), L201 (= L203), G203 (= G205), I204 (= I206), T205 (= T207), S240 (= S242), T241 (≠ S243)

3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
36% identity, 92% coverage: 14:275/285 of query aligns to 13:273/280 of 3lqsA

query
sites
3lqsA

D

E

E

E

A

V

R

K

V

I

S

D

P

K

M

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

A

V

Y
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Y

E

E

V
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V

V

V

P

K

V

V

Y

Y

S

N

R

G

R

E

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M

F

F

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A

N

E

E

H

H

L

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G

D

R
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R

L

L

A

Y

R

A

T

S

L

A

A

E

A

K

M

I

R

R

L

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P

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I

P

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H

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D

K

E

D

E

K

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F

A

H

D

Q

R

L

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L

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H

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E

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L

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V

A

E

G

K

N

N

P

E

W

L

E

N

D

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G

S

H

I

I

Y

Y

L

F

Q

Q

V

V

T

T

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R

G

G

A

T

D

S

A

P

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-

R

R

N

A

H

H

A

Q

F

F

P

P

K

E

D

N

-

T

V

V

R

K

P

P

T

V

V

I

F

I

M

G

L

Y

S

T

E

K

P

E

L

-

T

N

P

P

A

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P

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L

E

E

L

N

L

L

A

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S

K

G

G

V

V

A

K

A

A

V

T

S

F

A

V

A

E

D

D

F

I

R

R

W

W

L

L

R

R

C

C

D

D

L

I

K
|
K

S

S

V

L

S

N

L

L

A

G

N

A

C

V

L

L

L

A

R

K

Q

Q

Y

E

G

A

F

H

D

E

Q

K

G

G

C

C

A

Y

E

E

T

A

V

I

L

L

F

H

R

R

D

N

G

N

F

T

L

V

T

T

E
|
E

G

G

S

S

A
x
S

S
|
S

N
|
N

I

V

F

F

V

G

V

I

R

K

D

D

G

G

R

I

L

L

L

Y

A

T

P

H

P

P

K

A

S

N

H

N

L

M

M

I

L
|
L

A

K

G
|
G

I
|
I

T
|
T

Y

R

D

D

V

V

L

I

E

A

L

C

A

A

A

N

A

E

H

I

G

N

L

M

P

P

L

V

E

K

V

E

R

I

E

P

I

F

L

T

D

T

A

H

E

E

V

A

R

L

S

K

A

M

D

D

E

E

L

L

W

F

M

V

T

T

S
|
S

S
x
T

T
|
T

K

S

E

E

V

I

L

T

A

P

I

V

T

I

R

E

L

I

D

D

G

G

R

K

P

L

V

I

G

R

S

D

G

G

A

K

P

V

G

G

P

E

L

W

G

T

R

R

Q

K

M

L

Y

Q

A

K

W

Q

Y

F

Q

E

active site: Y31 (= Y32), V33 (= V34), K145 (= K147), E177 (= E179), L201 (= L203)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (= V34), R50 (= R51), E177 (= E179), S180 (≠ A182), S181 (= S183), N182 (= N184), L201 (= L203), G203 (= G205), I204 (= I206), T205 (= T207), S240 (= S242), T241 (≠ S243), T242 (= T244)

1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
36% identity, 92% coverage: 14:275/285 of query aligns to 13:273/282 of 1a0gB

query
sites
1a0gB

D

E

E

E

A

V

R

K

V

I

S

D

P

K

M

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

A

V

Y
|
Y

E

E

V
|
V

V

V

P

K

V

V

Y

Y

S

N

R

G

R

E

P

M

F

F

R

T

L

V

A

N

E

E

H

H

L

I

G

D

R
|
R

L

L

A

Y

R

A

T

S

L

A

A

E

A

K

M

I

R

R

L

I

P

T

N

I

P

P

H

Y

S

T

D

K

E

D

E

K

W

F

A

H

D

Q

R

L

V

L

T

H

T

E

L

L

V

V

A

E

G

K

N

N

P

E

W

L

E

N

D

T

Q

G

S

H

I

I

Y

Y

L

F

Q

Q

V

V

T

T

R

R

G

G

A

T

D

S

A

P

V

-

R

R

N

A

H

H

A

Q

F

F

P

P

K

E

D

N

-

T

V

V

R

K

P

P

T

V

V

I

F

I

M

G

L

Y

S

T

E

K

P

E

L

-

T

N

P

P

A

R

P

P

-

L

E

E

L

N

L

L

A

E

S

K

G

G

V

V

A

K

A

A

V

T

S

F

A

V

A

E

D

D

F

I

R

R

W

W

L

L

R

R

C

C

D

D

L

I

K
|
K

S

S

V

L

S

N

L

L

A

G

N

A

C

V

L

L

L

A

R

K

Q

Q

Y

E

G

A

F

H

D

E

Q

K

G

G

C

C

A

Y

E

E

T

A

V

I

L

L

F

H

R

R

D

N

G

N

F

T

L

V

T

T

E
|
E

G

G

S

S

A
x
S

S
|
S

N

N

I

V

F

F

V

G

V

I

R

K

D

D

G

G

R

I

L

L

L

Y

A

T

P

H

P

P

K

A

S

N

H

N

L

M

M

I

L
x
A

A

K

G
|
G

I
|
I

T
|
T

Y

R

D

D

V

V

L

I

E

A

L

C

A

A

A

N

A

E

H

I

G

N

L

M

P

P

L

V

E

K

V

E

R

I

E

P

I

F

L

T

D

T

A

H

E

E

V

A

R

L

S

K

A

M

D

D

E

E

L

L

W

F

M

V

T

T

S
|
S

S
x
T

T

T

K

S

E

E

V

I

L

T

A

P

I

V

T

I

R

E

L

I

D

D

G

G

R

K

P

L

V

I

G

R

S

D

G

G

A

K

P

V

G

G

P

E

L

W

G

T

R

R

Q

K

M

L

Y

Q

A

K

W

Q

Y

F

Q

E

active site: Y31 (= Y32), V33 (= V34), K145 (= K147), E177 (= E179), A201 (≠ L203)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R51), K145 (= K147), E177 (= E179), S180 (≠ A182), S181 (= S183), G203 (= G205), I204 (= I206), T205 (= T207), S240 (= S242), T241 (≠ S243)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
32% identity, 97% coverage: 1:277/285 of query aligns to 1:280/290 of 5mr0D

query
sites
5mr0D

M

M

N

L

L

Y

C

V

Y

Y

L

M

D

D

G

G

R

E

Y

F

L

V

P

P

L

E

D

N

E

E

A

A

R

K

V

V

S

S

P

I

M

F

D

D

R

H

G

G

F

F

L

L

F

Y

G

G

D

D

G

G

A

V

Y
x
F

E

E

V
x
G

V

I

P

R

V

A

Y

Y

S

N

R

G

R

R

P

V

F

F

R

R

L

L

A

K

E

E

H

H

L

I

G

D

R
|
R

L

L

A

Y

R

D

T

S

L

A

A

K

A

A

M

I

R

D

L

L

P

E

N

I

P

P

H

I

S

T

D

K

E

E

W

F

A

M

D

E

R

I

V

I

T

L

T

E

L

T

V

L

A

R

G

K

N

N

P

N

W

L

E

R

D

D

Q

A

S

Y

I

I

Y

R

L

P

Q

I

V

V

T

T

R

R

G

G

A

I

D

G

A

D

V

L

R

-

N

-

H
x
G

A
x
L

F

D

P

P

K

R

D

K

V

C

R

Q

-

N

P

P

T

S

V

I

F

I

M

V

L

I

S

T

E

K

P

P

L

W

L

G

T

K

P

L

A

Y

P

G

E

D

L

L

L

Y

A

E

S

K

G

G

V

L

A

T

A

A

V

I

S

T

A

V

A

A

-

V

-

R

-

N

D

S

F

F

R

D

W

A

L

L

R

P

C

P

D

N

L

I

K
|
K

S

S

V

L

S

N

L
x
Y

L

L

A

N

N

N

C

I

L

L

L

A

R

K

Q

I

Y

E

G

A

F

N

D

A

Q

K

G

G

C

G

A

D

E

E

T

A

V

I

L

F

F

L

-

D

R

R

D

N

G

G

F

Y

L

V

T

S

E
|
E

G

G

S

S

A
x
G

S
x
D

N

N

I

I

F

F

V

V

R

K

D

N

G

G

R

A

L

I

L

T

A

T

P

P

K

T

S

I

H

N

L

-

M

N

L
|
L

A

R

G

G

I
|
I

T
|
T

Y

R

D

E

V

A

V

V

L

I

E

E

L

I

A

I

A

N

A

R

H

L

G

G

L

I

P

P

L

F

E

K

V

E

R

T

E

N

I

I

L

G

D

L

A

Y

E

D

V

L

R

Y

S

T

A

A

D

D

E

E

L

V

W

F

M

V

T

T

S
x
G

S
x
T

T

A

K

A

E

E

V

I

L

A

A

P

I

I

T

V

R

V

L

I

D

D

G

G

R

R

P

K

V

I

G

G

S

D

G

G

A

K

P

P

G

G

P

E

L

I

G

T

R

R

Q

K

M

L

Y

M

A

E

W

E

Y

F

Q

S

E

K

F

L

active site: F32 (≠ Y32), G34 (≠ V34), K150 (= K147), E183 (= E179), L206 (= L203)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R51), G100 (≠ H102), L101 (≠ A103), K150 (= K147), Y154 (≠ L151), E183 (= E179), G186 (≠ A182), D187 (≠ S183), L206 (= L203), I209 (= I206), T210 (= T207), G245 (≠ S242), T246 (≠ S243)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
32% identity, 96% coverage: 3:276/285 of query aligns to 4:280/290 of 5e25A

query
sites
5e25A

L

L

C

V

Y

Y

L

M

D

N

G

G

R

E

Y

F

L

V

P

P

L

E

D

S

E

Q

A

A

R

K

V

V

S

S

P

V

M

F

D

D

R

H

G

G

F

F

L

L

F

Y

G

G

D

D

G

G

A

V

Y
x
F

E

E

V
x
G

V

I

P

R

V

A

Y

Y

S

N

R

G

R

K

P

V

F

F

R

K

L

L

A

Y

E

E

H

H

L

I

G

D

R
|
R

L

L

A

Y

R

D

T

C

L

A

A

R

A

V

M

I

R

D

L

L

P

K

N

I

P

P

H

L

S

S

D

K

E

E

W

F

A

A

D

E

R

A

V

I

T

L

T

E

L

T

V

L

A

R

G

R

N

N

P

N

W

L

E

R

D

D

Q

A

S
x
Y

I

I

Y

R

L

P

Q

I

V

V

T

T

R

R

G

G

A

A

D

G

A

D

V

L

R

-

N

-

H

G

A

L

F

D

P

P

K

R

D

K

V

C

-

P

R

S

P

P

T

N

V

V

F

I

M

I

L

I

S

T

E

K

P

P

L

W

L

G

T

K

P

L

A

Y

P

G

E

D

L

L

L

Y

A

E

S

K

G

G

V

L

A

K

A

A

V

I

S

T

A

V

A

A

D

I

F

R

R

R

-

N

-

A

-

I

-

D

W

S

L

L

R

P

C

P

D

N

L

I

K
|
K

S

S

V

L

S

N

L
x
Y

L

L

A

N

N

N

C

I

L

L

L

A

R

K

Q

I

Y

E

G

A

F

N

D

A

Q

K

G

G

C

G

A

D

E

E

T

A

V

I

L

F

F

L

-

D

R

H

D

N

G

G

F

Y

L

I

T

S

E
|
E

G

G

S

S

A
x
G

S
x
D

N

N

I

I

F

F

V

I

V

V

R

K

D

N

G

G

R

T

L

I

L

T

A

T

P

P

K

T

S

L

H

N

L

-

M

N

L
|
L

A

K

G
|
G

I
|
I

T
|
T

Y

R

D

Q

V

V

L

I

E

E

L

L

A

I

A

N

A

E

H

L

G

E

L

I

P

P

L

F

E

R

V

E

R

A

E

N

I

I

L

G

D

L

A

F

E

D

V

L

R

Y

S

S

A

A

D

D

E

E

L

I

W

F

M

V

T

T

S
x
G

S
x
T

T
x
A

K

A

E

E

V

I

L

A

A

P

I

V

T

T

R

Y

L

I

D

D

G

G

R

R

P

T

V

V

G

G

S

N

G

G

A

K

P

P

G

G

P

K

L

V

G

T

R

K

Q

M

M

L

Y

M

A

E

W

K

Y

F

Q

R

E

E

active site: F33 (≠ Y32), G35 (≠ V34), K151 (= K147), E184 (= E179), L207 (= L203)
binding 2-oxoglutaric acid: Y88 (≠ S87), K151 (= K147), T247 (≠ S243), A248 (≠ T244)
binding pyridoxal-5'-phosphate: R52 (= R51), K151 (= K147), Y155 (≠ L151), E184 (= E179), G187 (≠ A182), D188 (≠ S183), L207 (= L203), G209 (= G205), I210 (= I206), T211 (= T207), G246 (≠ S242), T247 (≠ S243)

7p3tB Transaminase of gamma-proteobacterium (see paper)
31% identity, 98% coverage: 3:280/285 of query aligns to 5:285/299 of 7p3tB

query
sites
7p3tB

L

I

C

I

Y

Y

L

I

D

N

G

G

R

D

Y

Y

L

L

P

P

L

L

D

S

E

Q

A

A

R

R

V

V

S

S

P

P

M

V

D

D

R

Q

G

G

F

F

L

L

F

L

G

G

D

D

G

G

A

V

Y

F

E

D

V

V

P

S

V

A

Y

W

S

K

R

G

R

N

P

I

F

F

R

K

L

L

A

D

E

A

H

H

L

L

G

D

R
|
R

L

F

A

F

R

D

T

S

L

I

A

Q

A

A

M

A

R

R

L

L

P

N

N

H

P

D

H

M

S

S

D

R

E

D

E

A

W

W

A

K

D

E

R

A

V

I

T

I

T

E

L

T

V

T

A

R

G

R

N

N

P

G

W

L

E

D

D

D

Q

A

S

S

I

I

Y

R

L

F

Q

I

V

V

T

T

R

R

G

G

A

-

D

E

A

P

V

K

R

G

N

V

H

V

A

A

F

D

P

P

K

R

D

D

V

F

R

K

P

P

T

T

V

C

F

I

M

V

L

W

S

V

E

A

P

P

L

Y

L

I

T

F

P

L

A

A

P

D

E

E

L

E

L

K

A

R

S

R

G

N

V

G

A

I

A

R

V

L

S

M

A

I

A

S

D

A

F

T

R

R

W

G

L

F

R

P

C

A

D

D

-

T

-

L

-

D

-

P

-

R

L

Y

K
|
K

S

C

V

L

S

D

L
x
R

L

L

A

H

N

S

C

Q

L

L

L

I

R

R

Q

L

Y

E

G

A

F

L

D

E

Q

A

G

G

C

Y

A

D

E

D

T

A

V

L

-

W

L

L

F

D

R

H

D

S

G

G

F

H

L

V

T

S

E
|
E

G
x
S

S
x
A

A
|
A

S
|
S

N

N

I

L

F

F

V

I

V

V

R

K

D

N

G

G

R

V

L

L

L

Y

A

T

P

P

P

-

K

-

S

S

H

A

L

G

M

I

L

L

A

R

G
|
G

I
|
I

T
|
T

Y

R

D

D

V

T

V

I

L

L

E

E

L

L

A

A

A

T

A

E

H

L

G

D

L

I

P

P

L

W

E

K

V

E

R

R

E

Q

I

L

L

S

D

A

A

F

E

D

V

V

R

Y

S

I

A

A

D

D

E

E

L

V

W

F

M

T

T

C

S

S

S
x
T

T

A

K

G

E

G

V

A

L

L

A

P

I

V

T

R

R

E

L

V

D

A

G

G

R

R

P

T

V

I

G

R

S

G

G

T

A

T

P

P

G

G

P

P

L

I

G

T

R

Q

Q

A

M

I

Y

D

A

N

W

A

Y

Y

Q

W

E

A

F

M

K

R

N

E

T

T

binding pyridoxal-5'-phosphate: R53 (= R51), K153 (= K147), R157 (≠ L151), E186 (= E179), S187 (≠ G180), A188 (≠ S181), A189 (= A182), S190 (= S183), G210 (= G205), I211 (= I206), T212 (= T207), T248 (≠ S243)

6xu3C (R)-selective amine transaminase from shinella sp. (see paper)
32% identity, 95% coverage: 3:274/285 of query aligns to 29:304/322 of 6xu3C

query
sites
6xu3C

L

I

C

A

Y

Y

L

I

D

A

G

G

R

E

Y

Y

L

V

P

P

L

L

D

H

E

E

A

A

R

R

V

I

S

P

P

I

M

L

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

A

T

Y
|
Y

E

D

V
|
V

V

P

P

A

V

V

Y

W

S

N

R

G

R

R

P

F

F

F

R

R

L

L

A

E

E

D

H

H

L

L

G

D

R
|
R

L

F

A

E

R

K

T

S

L

C

A

A

A

Q

M

L

R

R

L

L

P

K

N

S

P

P

H

L

S

S

D

R

E

E

W

I

A

R

D

D

R

R

V

L

T

V

T

E

L

M

V

T

A

V

G

K

N

S

P

G

W

I

E

R

D

D

Q

A

S

Y

I

V

Y

M

L

M

Q

I

V

V

T

T

R

R

G

G

A

L

D

R

A

F

V

V

R

R

N

Q

H

Y

A

A

F

-

P

P

K

E

D

E

V

C

R

D

P

N

T

F

V

C

F

Y

M

L

L

M

S

V

E

M

P

P

L

Y

L

L

T

W

P

V

A

M

P

D

E

E

L

A

L

T

-

Q

A

K

S

N

G

G

V

G

A

S

A

A

V

V

S

I

A

T

A

R

D

T

F

V

R

R

W

-

L

-

R

R

C

V

D

P

L

P

K

G

S

A

V

I

S

D

L

P

L

T

A

V

N
x
K

C

N

L

L

L

-

R

-

Q

Q

Y

W

G

G

-

D

F
|
F

D

T

Q

R

G

G

C

L

A

M

E

E

T

A

-

R

-

D

-

R

-

G

-

A

-

M

-

Y

-

P

-

I

V

L

L

T

F

D

R

G

D

D

G

A

F

N

L

L

T

T

E
|
E

G

G

S

S

A
x
G

S

F

N
|
N

I

V

F

I

V

L

V

I

R

K

D

D

G

G

R

K

L

L

L

Y

A

T

P

P

P

R

K

K

S

G

H

-

L

-

M

V

L
|
L

A

E

G
|
G

I
x
V

T
|
T

Y

R

D

K

V

S

V

V

L

L

E

A

L

V

A

A

A

E

A

K

H

L

G

G

L

Y

P

P

L

Y

E

T

V

I

R

D

E

D

I

V

L

P

D

V

A

E

E

L

V

A

R

Y

S

Q

A

C

D

D

E

E

L

I

W

L

M

F

T

V

S
x
T

T
x
A

K

G

E

G

V

V

L

M

A

P

I

I

T

T

R

T

L

L

D

D

G

G

R

Q

P

P

V

V

G

G

S

D

G

G

A

Q

P

V

G

G

P

P

L

I

G

S

R

K

Q

A

M

L

Y

W

A

K

W

G

Y

Y

active site: Y58 (= Y32), K178 (≠ N154), E211 (= E179), L233 (= L203)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: V60 (= V34), R77 (= R51), K178 (≠ N154), F185 (= F162), E211 (= E179), G214 (≠ A182), N216 (= N184), L233 (= L203), G235 (= G205), V236 (≠ I206), T237 (= T207), T272 (≠ S242), T273 (≠ S243), A274 (≠ T244)

6xu3A (R)-selective amine transaminase from shinella sp. (see paper)
32% identity, 95% coverage: 3:274/285 of query aligns to 27:302/320 of 6xu3A

query
sites
6xu3A

L

I

C

A

Y

Y

L

I

D

A

G

G

R

E

Y

Y

L

V

P

P

L

L

D

H

E

E

A

A

R

R

V

I

S

P

P

I

M

L

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

A

T

Y
|
Y

E

D

V

V

V

P

P

A

V

V

Y

W

S

N

R

G

R

R

P

F

F

F

R

R

L

L

A

E

E

D

H

H

L

L

G

D

R
|
R

L

F

A

E

R

K

T

S

L

C

A

A

A

Q

M

L

R

R

L

L

P

K

N

S

P

P

H

L

S

S

D

R

E

E

W

I

A

R

D

D

R

R

V

L

T

V

T

E

L

M

V

T

A

V

G

K

N

S

P

G

W

I

E

R

D

D

Q

A

S

Y

I

V

Y

M

L

M

Q

I

V

V

T

T

R

R

G

G

A

L

D

R

A

F

V

V

R

R

N

Q

H

Y

A

A

F

-

P

P

K

E

D

E

V

C

R

D

P

N

T

F

V

C

F

Y

M

L

L

M

S

V

E

M

P

P

L

Y

L

L

T

W

P

V

A

M

P

D

E

E

L

A

L

T

-

Q

A

K

S

N

G

G

V

G

A

S

A

A

V

V

S

I

A

T

A

R

D

T

F

V

R

R

W

-

L

-

R

R

C

V

D

P

L

P

K

G

S

A

V

I

S

D

L

P

L

T

A

V

N
x
K

C

N

L

L

L

-

R

-

Q

Q

Y

W

G

G

-

D

F
|
F

D

T

Q

R

G

G

C

L

A

M

E

E

T

A

-

R

-

D

-

R

-

G

-

A

-

M

-

Y

-

P

-

I

V

L

L

T

F

D

R

G

D

D

G

A

F

N

L

L

T

T

E
|
E

G

G

S

S

A
x
G

S
x
F

N

N

I

V

F

I

V

L

V

I

R

K

D

D

G

G

R

K

L

L

L

Y

A

T

P

P

P

R

K

K

S

G

H

-

L

-

M

V

L
|
L

A

E

G
|
G

I
x
V

T
|
T

Y

R

D

K

V

S

V

V

L

L

E

A

L

V

A

A

A

E

A

K

H

L

G

G

L

Y

P

P

L

Y

E

T

V

I

R

D

E

D

I

V

L

P

D

V

A

E

E

L

V

A

R

Y

S

Q

A

C

D

D

E

E

L

I

W

L

M

F

T

V

S

T

S
x
T

T

A

K

G

E

G

V

V

L

M

A

P

I

I

T

T

R

T

L

L

D

D

G

G

R

Q

P

P

V

V

G

G

S

D

G

G

A

Q

P

V

G

G

P

P

L

I

G

S

R

K

Q

A

M

L

Y

W

A

K

W

G

Y

Y

active site: Y56 (= Y32), K176 (≠ N154), E209 (= E179), L231 (= L203)
binding pyridoxal-5'-phosphate: R75 (= R51), K176 (≠ N154), F183 (= F162), E209 (= E179), G212 (≠ A182), F213 (≠ S183), L231 (= L203), G233 (= G205), V234 (≠ I206), T235 (= T207), T271 (≠ S243)

6xu3B (R)-selective amine transaminase from shinella sp. (see paper)
32% identity, 95% coverage: 3:274/285 of query aligns to 28:303/321 of 6xu3B

query
sites
6xu3B

L

I

C

A

Y

Y

L

I

D

A

G

G

R

E

Y

Y

L

V

P

P

L

L

D

H

E

E

A

A

R

R

V

I

S

P

P

I

M

L

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

A

T

Y
|
Y

E

D

V

V

V

P

P

A

V

V

Y

W

S

N

R

G

R

R

P

F

F

F

R

R

L

L

A

E

E

D

H

H

L

L

G

D

R
|
R

L

F

A

E

R

K

T

S

L

C

A

A

A

Q

M

L

R

R

L

L

P

K

N

S

P

P

H

L

S

S

D

R

E

E

W

I

A

R

D

D

R

R

V

L

T

V

T

E

L

M

V

T

A

V

G

K

N

S

P

G

W

I

E

R

D

D

Q

A

S
x
Y

I

V

Y

M

L

M

Q

I

V

V

T

T

R

R

G

G

A

L

D

R

A

F

V

V

R

R

N

Q

H

Y

A

A

F

-

P

P

K

E

D

E

V

C

R

D

P

N

T

F

V

C

F

Y

M

L

L

M

S

V

E

M

P

P

L

Y

L

L

T

W

P

V

A

M

P

D

E

E

L

A

L

T

-

Q

A

K

S

N

G

G

V

G

A

S

A

A

V

V

S

I

A

T

A

R

D

T

F

V

R

R

W

-

L

-

R

R

C

V

D

P

L
x
P

K

G

S

A

V

I

S
x
D

L

P

L

T

A

V

N
x
K

C

N

L

L

L

-

R

-

Q

Q

Y

W

G

G

-

D

F
|
F

D

T

Q

R

G

G

C

L

A

M

E

E

T

A

-

R

-

D

-

R

-

G

-

A

-

M

-

Y

-

P

-

I

V

L

L

T

F

D

R

G

D

D

G

A

F

N

L

L

T

T

E
|
E

G

G

S

S

A
x
G

S
x
F

N
|
N

I

V

F

I

V

L

V

I

R

K

D

D

G

G

R

K

L

L

L

Y

A

T

P

P

P

R

K
|
K

S

G

H

-

L

-

M

V

L
|
L

A

E

G
|
G

I
x
V

T
|
T

Y

R

D

K

V

S

V

V

L

L

E

A

L

V

A

A

A

E

A

K

H

L

G

G

L

Y

P

P

L

Y

E

T

V

I

R

D

E

D

I

V

L

P

D

V

A

E

E

L

V

A

R

Y

S

Q

A

C

D

D

E

E

L

I

W

L

M

F

T

V

S

T

S
x
T

T

A

K

G

E

G

V

V

L

M

A

P

I

I

T

T

R

T

L

L

D

D

G

G

R

Q

P

P

V

V

G

G

S

D

G

G

A

Q

P

V

G

G

P

P

L

I

G

S

R

K

Q

A

M

L

Y

W

A

K

W

G

Y

Y

active site: Y57 (= Y32), K177 (≠ N154), E210 (= E179), L232 (= L203)
binding 3-aminobenzoic acid: P169 (≠ L146), D173 (≠ S150), K229 (= K198)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: R76 (= R51), Y112 (≠ S87), K177 (≠ N154), F184 (= F162), E210 (= E179), G213 (≠ A182), F214 (≠ S183), N215 (= N184), L232 (= L203), G234 (= G205), V235 (≠ I206), T236 (= T207), T272 (≠ S243)

8onjA Crystal structure of d-amino acid aminotransferase from aminobacterium colombiense point mutant r88l (see paper)
31% identity, 96% coverage: 1:274/285 of query aligns to 3:268/277 of 8onjA

query
sites
8onjA

M

M

N

N

L

L

C

C

Y

Y

L

I

D

D

G

G

R

K

Y

F

L

L

P

P

L

L

D

E

E

E

A

A

R

K

V

L

S

P

P

V

M

T

D

D

R

L

G

I

F

I

L

Q

F

R

G

G

D

V

G

G

A

V

Y

F

E

E

V

T

V

I

P

S

V

T

Y

H

S

S

R

R

P

P

F

L

R

M

L

L

A

T

E

P

H

H

L

L

G

K

R
|
R

L

L

A

E

R

G

T

S

L

A

A

T

A

A

M

S

R

S

L

I

P

V

N

M

P

P

H

A

S

T

D

L

E

D

E

E

W

M

A

A

D

R

R

I

V

I

T

R

T

E

L

G

V

I

A

K

G

K

N

M

P

G

W

C

E

E

D

-

Q

-

S

T

I

M

Y

V

L

L

Q

P

V

Y

T

I

R

T

G

G

A

G

D

D

A

S

V

F

-

G

R

K

N

D

H

H

A

L

F

F

P

S

K

S

D

S

V

-

R

-

P

-

T

R

V

Y

F

F

M

V

L

I

S

F

E

E

P

E

L

I

L

R

T

K

P

P

A

D

P

P

E

I

L

L

L

Y

A

E

S

K

G

G

V

V

A

A

A

L

V

H

S

P

A

I

A

N

D

A

F

E

R
|
R

W

Y

L

L

R

P

C

S

D

T

L

-

K
|
K

S

S

V

I

S

-

L

-

A

-

N

N

C

Y

L

M

L

L

R

S

Q

F

Y

T

G

G

F

Q

-

R

-

D

D

S

Q

K

G

G

C

A

A

Y

E

E

T

I

V

L

L

Y

F

C

R

P

D

E

G

G

F

E

L

I

T

V

E
|
E

G

G

S

S

A
x
H

S
|
S

N

T

I

F

F

F

V

L

V

I

R

K

D

N

G

G

R

H

L

L

L

I

A

T

P

A

P

P

K

T

S

S

H

R

L

-

M

A

L
|
L

A

S

G

G

I
x
T

T
|
T

Y

R

D

Q

V

I

V

V

L

L

E

E

L

L

A

A

A

R

H

G

G

N

L

I

P

Q

L

V

E

E

V

E

R

R

E

C

I

P

L

L

D

L

A

T

E

E

V

L

R

P

S

E

A

A

D

E

E

E

L

A

W

F

M

I

T

T

S
x
G

S
x
T

T

V

K

K

E

E

V

L

L

L

A

P

I

V

T

V

R

R

L

I

D

G

G

D

R

Q

P

I

V

I

G

G

S

N

G

G

A

V

P

P

G

G

P

K

L

L

G

T

R

K

Q

H

M

L

Y

H

A

Q

W

V

Y

Y

binding pyridoxal-5'-phosphate: R53 (= R51), R138 (= R140), K144 (= K147), E174 (= E179), H177 (≠ A182), S178 (= S183), L197 (= L203), T200 (≠ I206), T201 (= T207), G236 (≠ S242), T237 (≠ S243)

8aykA Crystal structure of d-amino acid aminotrensferase from aminobacterium colombiense complexed with d-glutamate (see paper)
31% identity, 96% coverage: 1:274/285 of query aligns to 2:267/276 of 8aykA

query
sites
8aykA

M

M

N

N

L

L

C

C

Y

Y

L

I

D

D

G

G

R

K

Y

F

L

L

P

P

L

L

D

E

E

E

A

A

R

K

V

L

S

P

P

V

M

T

D

D

R

L

G

I

F

I

L

Q

F

R

G

G

D

V

G

G

A

V

Y

F

E

E

V
x
T

V

I

P

S

V

T

Y

H

S

S

R

R

P

P

F

L

R

M

L

L

A

T

E

P

H

H

L

L

G

K

R
|
R

L

L

A

E

R

G

T

S

L

A

A

T

A

A

M

S

R

S

L

I

P

V

N

M

P

P

H

A

S

T

D

L

E

D

E

E

W

M

A

A

D

R

R

I

V

I

T

R

T

E

L

G

V

I

A

K

G

K

N

M

P

G

W

C

E

E

D

T

Q

M

S

-

I

V

Y

R

L

P

Q

Y

V

I

T

T

R

G

G

G

A

D

D

S

A

F

V

G

R

K

N

D

H

H

A

L

F

F

P

S

K

S

D

S

V

-

R

-

P

-

T

R

V

Y

F

F

M

V

L

I

S

F

E

E

P

E

L

I

L

R

T

K

P

P

A

D

P

P

E

I

L

L

L

Y

A

E

S

K

G

G

V

V

A

A

A

L

V

H

S

P

A

I

A

N

D

A

F

E

R
|
R

W

Y

L

L

R

P

C

S

D

T

L

-

K
|
K

S

S

V

I

S

-

L

-

A

-

N

N

C

Y

L

M

L

L

R

S

Q

F

Y

T

G

G

F

Q

-

R

-

D

D

S

Q

K

G

G

C

A

A

Y

E

E

T

I

V

L

L

Y

F

C

R

P

D

E

G

G

F

E

L

I

T

V

E
|
E

G

G

S
|
S

A
x
H

S
|
S

N

T

I

F

F

F

V

L

V

I

R

K

D

N

G

G

R

H

L

L

L

I

A

T

P

A

P

P

K

T

S

S

H

R

L

-

M

A

L
|
L

A

S

G
|
G

I
x
T

T
|
T

Y

R

D

Q

V

I

V

V

L

L

E

E

L

L

A

A

A

R

H

G

G

N

L

I

P

Q

L

V

E

E

V

E

R

R

E

C

I

P

L

L

D

L

A

T

E

E

V

L

R

P

S

E

A

A

D

E

E

E

L

A

W

F

M

I

T

T

S
x
G

S
x
T

T
x
V

K
|
K

E

E

V

L

L

L

A

P

I

V

T

V

R

R

L

I

D

G

G

D

R

Q

P

I

V

I

G

G

S

N

G

G

A

V

P

P

G

G

P

K

L

L

G

T

R

K

Q

H

M

L

Y

H

A

Q

W

V

Y

Y

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R52 (= R51), R137 (= R140), K143 (= K147), E173 (= E179), S175 (= S181), H176 (≠ A182), S177 (= S183), L196 (= L203), T199 (≠ I206), T200 (= T207), G235 (≠ S242), T236 (≠ S243)
binding (~{Z})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pent-2-enedioic acid: T35 (≠ V34), R52 (= R51), R137 (= R140), K143 (= K147), E173 (= E179), H176 (≠ A182), S177 (= S183), L196 (= L203), G198 (= G205), T199 (≠ I206), T200 (= T207), G235 (≠ S242), T236 (≠ S243), V237 (≠ T244), K238 (= K245)

8ayjA Crystal structure of d-amino acid aminotransferase from aminobacterium colombiens complexed with 3-aminooxypropionic acid (see paper)
31% identity, 96% coverage: 1:274/285 of query aligns to 2:267/276 of 8ayjA

query
sites
8ayjA

M

M

N

N

L

L

C

C

Y

Y

L

I

D

D

G

G

R

K

Y

F

L

L

P

P

L

L

D

E

E

E

A

A

R

K

V

L

S

P

P

V

M

T

D

D

R

L

G

I

F

I

L

Q

F

R

G

G

D

V

G

G

A

V

Y

F

E

E

V
x
T

V

I

P

S

V

T

Y

H

S

S

R

R

P

P

F

L

R

M

L

L

A

T

E

P

H

H

L

L

G

K

R
|
R

L

L

A

E

R

G

T

S

L

A

A

T

A

A

M

S

R

S

L

I

P

V

N

M

P

P

H

A

S

T

D

L

E

D

E

E

W

M

A

A

D

R

R

I

V

I

T

R

T

E

L

G

V

I

A

K

G

K

N

M

P

G

W

C

E

E

D

T

Q
x
M

S

-

I

V

Y
x
R

L

P

Q

Y

V

I

T

T

R

G

G

G

A

D

D

S

A

F

V

G

R

K

N

D

H

H

A

L

F

F

P

S

K

S

D

S

V

-

R

-

P

-

T

R

V

Y

F

F

M

V

L

I

S

F

E

E

P

E

L

I

L

R

T

K

P

P

A

D

P

P

E

I

L

L

L

Y

A

E

S

K

G

G

V

V

A

A

A

L

V

H

S

P

A

I

A

N

D

A

F

E

R
|
R

W

Y

L

L

R

P

C

S

D

T

L

-

K
|
K

S

S

V

I

S

-

L

-

A

-

N

N

C
x
Y

L

M

L

L

R

S

Q

F

Y

T

G

G

F

Q

-

R

-

D

D

S

Q

K

G

G

C

A

A

Y

E

E

T

I

V

L

L

Y

F

C

R

P

D

E

G

G

F

E

L

I

T

V

E
|
E

G

G

S
|
S

A
x
H

S
|
S

N

T

I

F

F

F

V

L

V

I

R

K

D

N

G

G

R

H

L

L

L

I

A

T

P

A

P

P

K

T

S

S

H

R

L

-

M

A

L
|
L

A

S

G
|
G

I
x
T

T
|
T

Y

R

D

Q

V

I

V

V

L

L

E

E

L

L

A

A

A

R

H

G

G

N

L

I

P

Q

L

V

E

E

V

E

R

R

E

C

I

P

L

L

D

L

A

T

E

E

V

L

R

P

S

E

A

A

D

E

E

E

L

A

W

F

M

I

T

T

S
x
G

S
x
T

T
x
V

K
|
K

E

E

V

L

L

L

A

P

I

V

T

V

R

R

L

I

D

G

G

D

R

Q

P

I

V

I

G

G

S

N

G

G

A

V

P

P

G

G

P

K

L

L

G

T

R

K

Q

H

M

L

Y

H

A

Q

W

V

Y

Y

binding 3-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxypropanoic acid: T35 (≠ V34), T35 (≠ V34), R52 (= R51), M87 (≠ Q86), R89 (≠ Y89), R137 (= R140), K143 (= K147), Y147 (≠ C155), Y147 (≠ C155), E173 (= E179), H176 (≠ A182), H176 (≠ A182), S177 (= S183), L196 (= L203), G198 (= G205), T199 (≠ I206), T200 (= T207), G235 (≠ S242), T236 (≠ S243), T236 (≠ S243), V237 (≠ T244), K238 (= K245)
binding pyridoxal-5'-phosphate: R52 (= R51), R137 (= R140), Y147 (≠ C155), E173 (= E179), S175 (= S181), H176 (≠ A182), S177 (= S183), L196 (= L203), G198 (= G205), T199 (≠ I206), T200 (= T207), T236 (≠ S243)

8aieA Crystal structure of d-amino acid aminotransferase from aminobacterium colombiense complexed with d-cycloserine
31% identity, 96% coverage: 1:274/285 of query aligns to 2:267/276 of 8aieA

query
sites
8aieA

M

M

N

N

L

L

C

C

Y

Y

L

I

D

D

G

G

R

K

Y

F

L

L

P

P

L

L

D

E

E

E

A

A

R

K

V

L

S

P

P

V

M

T

D

D

R

L

G

I

F

I

L

Q

F

R

G

G

D

V

G

G

A

V

Y

F

E

E

V
x
T

V

I

P

S

V

T

Y

H

S

S

R

R

P

P

F

L

R

M

L

L

A

T

E

P

H

H

L

L

G

K

R
|
R

L

L

A

E

R

G

T

S

L

A

A

T

A

A

M

S

R

S

L

I

P

V

N

M

P

P

H

A

S

T

D

L

E

D

E

E

W

M

A

A

D

R

R

I

V

I

T

R

T

E

L

G

V

I

A

K

G

K

N

M

P

G

W

C

E

E

D

T

Q

M

S

-

I

V

Y

R

L

P

Q

Y

V

I

T

T

R

G

G

G

A

D

D

S

A

F

V

G

R

K

N

D

H

H

A

L

F

F

P

S

K

S

D

S

V

-

R

-

P

-

T

R

V

Y

F

F

M

V

L

I

S

F

E

E

P

E

L

I

L

R

T

K

P

P

A

D

P

P

E

I

L

L

L

Y

A

E

S

K

G

G

V

V

A

A

A

L

V

H

S

P

A

I

A

N

D

A

F

E

R
|
R

W

Y

L

L

R

P

C

S

D

T

L

-

K
|
K

S

S

V

I

S

-

L

-

A

-

N

N

C
x
Y

L

M

L

L

R

S

Q

F

Y

T

G

G

F

Q

-

R

-

D

D

S

Q

K

G

G

C

A

A

Y

E

E

T

I

V

L

L

Y

F

C

R

P

D

E

G

G

F

E

L

I

T

V

E
|
E

G

G

S
|
S

A
x
H

S
|
S

N

T

I

F

F

F

V

L

V

I

R

K

D

N

G

G

R

H

L

L

L

I

A

T

P

A

P

P

K

T

S

S

H

R

L

-

M

A

L
|
L

A

S

G
|
G

I
x
T

T
|
T

Y

R

D

Q

V

I

V

V

L

L

E

E

L

L

A

A

A

R

H

G

G

N

L

I

P

Q

L

V

E

E

V

E

R

R

E

C

I

P

L

L

D

L

A

T

E

E

V

L

R

P

S

E

A

A

D

E

E

E

L

A

W

F

M

I

T

T

S
x
G

S
x
T

T
x
V

K
|
K

E

E

V

L

L

L

A

P

I

V

T

V

R

R

L

I

D

G

G

D

R

Q

P

I

V

I

G

G

S

N

G

G

A

V

P

P

G

G

P

K

L

L

G

T

R

K

Q

H

M

L

Y

H

A

Q

W

V

Y

Y

binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: T35 (≠ V34), R52 (= R51), K143 (= K147), Y147 (≠ C155), S175 (= S181), H176 (≠ A182), S177 (= S183), T199 (≠ I206), T200 (= T207), G235 (≠ S242), T236 (≠ S243), V237 (≠ T244), K238 (= K245)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R52 (= R51), R137 (= R140), K143 (= K147), Y147 (≠ C155), E173 (= E179), S177 (= S183), L196 (= L203), G198 (= G205), T199 (≠ I206), T200 (= T207), G235 (≠ S242), T236 (≠ S243)

8aieB Crystal structure of d-amino acid aminotransferase from aminobacterium colombiense complexed with d-cycloserine
31% identity, 96% coverage: 1:274/285 of query aligns to 1:266/275 of 8aieB

query
sites
8aieB

M

M

N

N

L

L

C

C

Y

Y

L

I

D

D

G

G

R

K

Y

F

L

L

P

P

L

L

D

E

E

E

A

A

R

K

V

L

S

P

P

V

M

T

D

D

R

L

G

I

F

I

L

Q

F

R

G

G

D

V

G

G

A

V

Y

F

E

E

V
x
T

V

I

P

S

V

T

Y

H

S

S

R

R

P

P

F

L

R

M

L

L

A

T

E

P

H

H

L

L

G

K

R
|
R

L

L

A

E

R

G

T

S

L

A

A

T

A

A

M

S

R

S

L

I

P

V

N

M

P

P

H

A

S

T

D

L

E

D

E

E

W

M

A

A

D

R

R

I

V

I

T

R

T

E

L

G

V

I

A

K

G

K

N

M

P

G

W

C

E

E

D

T

Q

M

S

-

I

V

Y

R

L

P

Q

Y

V

I

T

T

R

G

G

G

A

D

D

S

A

F

V

G

R

K

N

D

H

H

A

L

F

F

P

S

K

S

D

S

V

-

R

-

P

-

T

R

V

Y

F

F

M

V

L

I

S

F

E

E

P

E

L

I

L

R

T

K

P

P

A

D

P

P

E

I

L

L

L

Y

A

E

S

K

G

G

V

V

A

A

A

L

V

H

S

P

A

I

A

N

D

A

F

E

R

R

W

Y

L

L

R

P

C

S

D

T

L

-

K
|
K

S

S

V

I

S

-

L

-

A

-

N

N

C
x
Y

L

M

L

L

R

S

Q

F

Y

T

G

G

F

Q

-

R

-

D

D

S

Q

K

G

G

C

A

A

Y

E

E

T

I

V

L

L

Y

F

C

R

P

D

E

G

G

F

E

L

I

T

V

E
|
E

G

G

S
|
S

A
x
H

S
|
S

N

T

I

F

F

F

V

L

V

I

R

K

D

N

G

G

R

H

L

L

L

I

A

T

P

A

P

P

K

T

S

S

H

R

L

-

M

A

L
|
L

A

S

G

G

I
x
T

T
|
T

Y

R

D

Q

V

I

V

V

L

L

E

E

L

L

A

A

A

R

H

G

G

N

L

I

P

Q

L

V

E

E

V

E

R

R

E

C

I

P

L

L

D

L

A

T

E

E

V

L

R

P

S

E

A

A

D

E

E

E

L

A

W

F

M

I

T

T

S
x
G

S
x
T

T
x
V

K
|
K

E

E

V

L

L

L

A

P

I

V

T

V

R

R

L

I

D

G

G

D

R

Q

P

I

V

I

G

G

S

N

G

G

A

V

P

P

G

G

P

K

L

L

G

T

R

K

Q

H

M

L

Y

H

A

Q

W

V

Y

Y

binding 3-azanyloxy-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]propanoic acid: T34 (≠ V34), R51 (= R51), K142 (= K147), Y146 (≠ C155), E172 (= E179), H175 (≠ A182), S176 (= S183), T198 (≠ I206), T199 (= T207), G234 (≠ S242), T235 (≠ S243), V236 (≠ T244), K237 (= K245)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R51 (= R51), Y146 (≠ C155), E172 (= E179), S174 (= S181), H175 (≠ A182), S176 (= S183), L195 (= L203), T198 (≠ I206), T199 (= T207), G234 (≠ S242), T235 (≠ S243)

6snlD (R)-selective amine transaminase from exophiala sideris (see paper)
30% identity, 96% coverage: 2:274/285 of query aligns to 27:304/320 of 6snlD

query
sites
6snlD

N

G

L

I

C

A

Y

W

L

V

D

F

G

G

R

E

Y

L

L

V

P

P

L

L

D

A

E

E

A

A

R

R

V

I

S

P

P

L

M

M

D

D

R

Q

G

G

F

F

L

M

F

H

G

S

D

D

G

L

A

T

Y
|
Y

E

D

V

V

V

P

P

S

V

V

Y

W

S

D

R

G

R

R

P

F

F

F

R

R

L

L

A

D

E

D

H

H

L

I

G

A

R
|
R

L

L

A

E

R

A

T

S

L

C

A

A

A

K

M

M

R

R

L

L

P

Q

N

L

P

P

H

L

S

P

D

R

E

E

W

V

A

K

D

Q

R

I

V

L

T

V

T

D

L

M

V

V

A

A

G

K

N

S

P

E

W

I

E

K

D

D

Q

A

S

F

I

V

Y

E

L

L

Q

I

V

V

T

T

R

R

G

G

A

L

D

K

A

G

V

V

R

R

N

G

H

H

A

T

F

P

P

G

K

E

D

T

V

F

R

K

P

N

T

H

V

L

F

Y

M

M

L

F

S

V

E

Q

P

P

L

Y

L

V

-

W

T

V

P

M

A

D

P

P

E

D

L

I

L

Q

A

K

S

T

G

G

V

G

A

K

A

A

V

I

S

I

A

A

A

R

D

T

F

V

R

R

W

R

L

I

-

P

-

G

R

S

C

I

D

D

L

P

K

T

S

V

V
x
K

S

N

L

L

L

Q

A

W

N

G

C

D

L

L

L

T

R

R

Q

G

Y

L

-

F

-

E

G

A

F

A

D

D

Q

R

G

G

C

A

A

T

E

Y

T

P

V

F

L

L

F

T

R

D

-

G

D

D

G

A

F

N

L

L

T

T

E
|
E

G

G

S

S

A
x
G

S
x
F

N

N

I

V

F

L

V

L

V

V

R

K

D

D

G

G

R

V

L

I

L

Y

A

T

P

P

P

D

K

R

S

G

H

-

L

-

M

V

L
|
L

A

Q

G
|
G

I
x
V

T
|
T

Y

R

D

K

V

S

V

C

L

I

E

D

L

V

A

A

A

K

A

S

H

L

G

G

L

I

P

E

L

V

E

R

V

V

R

Q

E

F

I

V

L

P

D

I

A

Q

E

M

V

A

R

Y

S

D

A

A

D

D

E

E

L

I

W

F

M

M

T

A

S

T

S
x
T

T

A

K

G

E

G

V

I

L

M

A

P

I

I

T

T

R

T

L

L

D

D

G

D

R

K

P

P

V

I

G

Q

S

S

G

G

A

K

P

V

G

G

P

P

L

I

G

T

R

K

Q

K

M

I

Y

W

A

D

W

G

Y

Y

active site: Y57 (= Y32), K178 (≠ V149), E211 (= E179), L233 (= L203)
binding pyridoxal-5'-phosphate: R76 (= R51), K178 (≠ V149), E211 (= E179), G214 (≠ A182), F215 (≠ S183), L233 (= L203), G235 (= G205), V236 (≠ I206), T237 (= T207), T273 (≠ S243)

6xwbA Crystal structure of an r-selective transaminase from thermomyces stellatus. (see paper)
32% identity, 97% coverage: 2:277/285 of query aligns to 28:307/319 of 6xwbA

query
sites
6xwbA

N

G

L

I

C

A

Y

W

L

V

D

E

G

G

R

E

Y

M

L

V

P

P

L

L

D

H

E

E

A

A

R

R

V

I

S

P

P

M

M

L

D

D

R

E

G

G

F

F

L

M

F

R

G

S

D

D

G

L

A

T

Y

Y

E

D

V

V

V

P

P

S

V

V

Y

W

S

D

R

G

R

R

P

F

F

F

R

R

L

L

A

D

E

D

H

H

L

L

G

S

R
|
R

L

L

A

E

R

A

T

S

L

C

A

A

A

K

M

L

R

R

L

L

P

K

N

L

P

P

H

L

S

P

D

R

E

E

W

V

A

K

D

K

R

I

V

L

T

V

T

E

L

M

V

V

A

A

G

K

N

S

P

G

W

I

E

R

D

D

Q

A

S

F

I

V

Y

E

L

I

Q

I

V

V

T

T

R

R

G

G

A

L

D

K

A

G

V

V

R

R

N

G

H

-

A

S

F

R

P

P

K

E

D

E

V

I

R

V

P

N

T

R

V

L

F

Y

M

M

L

L

S

V

E

Q

P

P

L

Y

L

V

-

W

T

V

P

M

A

E

P

P

E

E

L

V

L

Q

A

P

S

V

G

G

V

G

A

D

A

A

V

V

S

I

A

A

A

R

D

T

F

V

R

R

W

-

L

-

R

R

C

V

D

P

L

P

K

G

S

S

V

I

S

D

L

P

L

T

A

V

N
x
K

C

N

L

L

L

-

R

-

Q

Q

Y

W

G

G

-

D

-
x
F

-

V

-

R

-

G

-

L

-

F

-

E

-

A

F

S

D

D

Q

R

G

G

C

A

A

T

E

Y

T

P

V

F

L

L

F

T

R

D

-

G

D

D

G

A

F

N

L

L

T

T

E
|
E

G

G

S

S

A
x
G

S
x
F

N
|
N

I

I

F

V

V

L

V

V

R

K

D

D

G

G

R

V

L

L

L

Y

A

T

P

P

P

D

K

R

S

G

H

-

L

-

M

V

L
|
L

A

Q

G
|
G

I
x
V

T
|
T

Y

R

D

K

V

S

V

V

L

I

E

D

L

V

A

A

A

N

A

A

H

K

G

G

L

F

P

E

L

V

E

R

V

V

R

E

E

Y

I

V

L

P

D

V

A

E

E

A

V

A

R

Y

S

H

A

A

D

D

E

E

L

I

W

F

M

M

T

C

S

T

S
x
T

T

A

K

G

E

G

V

I

L

M

A

P

I

I

T

R

R

S

L

L

D

D

G

G

R

K

P

P

V

V

G

N

S

D

G

G

A

K

P

V

G

G

P

P

L

I

G

T

R

K

Q

A

M

I

Y

W

A

D

W

G

Y

Y

Q

W

E

E

F

M

binding pyridoxal-5'-phosphate: R77 (= R51), K178 (≠ N154), F185 (vs. gap), E211 (= E179), G214 (≠ A182), F215 (≠ S183), N216 (= N184), L233 (= L203), G235 (= G205), V236 (≠ I206), T237 (= T207), T273 (≠ S243)

6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
29% identity, 95% coverage: 3:274/285 of query aligns to 29:305/321 of 6fteB

query
sites
6fteB

L

V

C

A

Y

W

L

V

D

E

G

G

R

K

Y

L

L

V

P

P

L

V

D

N

E

E

A

A

R

R

V

I

S

P

P

L

M

M

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

A

T

Y
|
Y

E

D

V

V

V

P

P

S

V

V

Y

W

S

D

R

G

R

R

P

F

F

F

R

R

L

L

A

D

E

D

H

H

L

L

G

D

R
|
R

L

F

A

E

R

L

T

S

L

C

A

S

A

K

M

M

R

R

L

F

P

K

N

M

P

P

H

L

S

P

D

R

E

Q

E

E

W

V

A

K

D

R

R

I

V

L

T

V

T

D

L

M

V

V

A

A

G

K

N

S

P

G

W

I

E

K

D

D

Q

A

S

F

I

V

Y

E

L

I

Q

I

V

V

T

T

R

R

G

G

A

L

D

K

A

G

V

V

R

R

N

G

H

L

A

K

F

A

P

G

K

E

D

S

V

L

R

T

P

N

T

N

V

L

F

Y

M

M

L

W

S

I

E

Q

P

P

L

Y

L

I

-

W

T

V

P

M

A

E

P

P

E

E

L

M

L

Q

A

R

S

T

G

G

V

G

A

S

A

A

V

I

S

I

A

A

A

R

D

T

F

V

R

K

W

-

L

-

R

R

C

T

D

S

L

P

K

G

S

S

V

M

S

D

L

P

L

T

A

V

N
x
K

C

N

L

L

L

-

R

-

Q

Q

Y

W

G

G

-

D

-

L

-

T

-

R

-

G

-

M

-

L

-

E

-

A

F

Q

D

D

Q

R

G

G

C

A

A

D

E

Y

T

P

V

F

L

L

F

T

R

D

-

G

D

D

G

G

F

N

L

I

T

T

E
|
E

G

G

S

S

A

G

S
x
F

N
|
N

I

I

F

V

V

F

V

I

R

K

D

D

G

G

R

V

L

L

L

Y

A

T

P

P

P

D

K

R

S

G

H

-

L

-

M

V

L
|
L

A

K

G
|
G

I
x
V

T
|
T

Y

R

D

K

V

S

V

V

L

A

E

D

L

A

A

A

A

K

A

A

H

N

G

G

L

I

P

E

L

M

E

R

V

I

R

E

E

F

I

V

L

P

D

T

A

E

E

M

V

A

R

Y

S

Q

A

C

D

D

E

E

L

C

W

F

M

M

T

C

S

T

S
x
T

T

A

K

G

E

G

V

V

L

M

A

P

I

I

T

T

R

S

L

M

D

D

G

G

R

Q

P

P

V

I

G

G

S

D

G

G

A

K

P

V

G

G

P

P

L

V

G

T

R

K

Q

E

M

I

Y

W

A

D

W

G

Y

Y

active site: Y58 (= Y32), K179 (≠ N154), E212 (= E179), L234 (= L203)
binding pyridoxal-5'-phosphate: R77 (= R51), K179 (≠ N154), E212 (= E179), F216 (≠ S183), N217 (= N184), L234 (= L203), G236 (= G205), V237 (≠ I206), T238 (= T207), T274 (≠ S243)

Query Sequence

>WP_011763917.1 NCBI__GCF_000061505.1:WP_011763917.1
MNLCYLDGRYLPLDEARVSPMDRGFLFGDGAYEVVPVYSRRPFRLAEHLGRLARTLAAMR
LPNPHSDEEWADRVTTLVAGNPWEDQSIYLQVTRGADAVRNHAFPKDVRPTVFMLSEPLL
TPAPELLASGVAAVSAADFRWLRCDLKSVSLLANCLLRQYGFDQGCAETVLFRDGFLTEG
SASNIFVVRDGRLLAPPKSHLMLAGITYDVVLELAAAHGLPLEVREILDAEVRSADELWM
TSSTKEVLAITRLDGRPVGSGAPGPLGRQMYAWYQEFKNTVMRSG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory