SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011765343.1 NCBI__GCF_000061505.1:WP_011765343.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3fslA Crystal structure of tyrosine aminotransferase tripple mutant (p181q, r183g,a321k) from escherichia coli at 2.35 a resolution
53% identity, 99% coverage: 5:400/402 of query aligns to 1:397/397 of 3fslA

query
sites
3fslA

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active site: W131 (= W134), D212 (= D215), A214 (= A217), K247 (= K250)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G103 (= G106), G104 (= G107), S105 (≠ T108), W131 (= W134), N184 (= N187), D212 (= D215), A214 (= A217), Y215 (= Y218), S244 (= S247), S246 (= S249), K247 (= K250), R255 (= R258)

3tatA Tyrosine aminotransferase from e. Coli (see paper)
53% identity, 99% coverage: 5:400/402 of query aligns to 1:397/397 of 3tatA

query
sites
3tatA

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active site: W131 (= W134), D212 (= D215), A214 (= A217), K247 (= K250)
binding pyridoxal-5'-phosphate: G103 (= G106), G104 (= G107), S105 (≠ T108), H179 (= H182), N184 (= N187), A214 (= A217), S246 (= S249), K247 (= K250), R255 (= R258)

6zvgA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with substrate analog - l-indole-3-lactic acid (see paper)
47% identity, 99% coverage: 5:401/402 of query aligns to 1:397/398 of 6zvgA

query
sites
6zvgA

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binding 3-(indol-3-yl) lactate: D11 (= D15), I13 (= I17), L14 (= L18), I33 (≠ V37), G34 (= G38), W130 (= W134), K246 (= K250), R374 (= R378)
binding pyridoxal-5'-phosphate: G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), N183 (= N187), D211 (= D215), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)

6zurA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with substrate analog - l-p-hydroxyphenyllactic acid (see paper)
47% identity, 99% coverage: 5:401/402 of query aligns to 1:397/398 of 6zurA

query
sites
6zurA

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A

C

V

V

R

K

A

I

A

A

E

E

K

Q

A

R

R

I

L

A

E

D

A

P

M

I

P

S

P

P

R

R

G

P

Y

Y

Q

L

P

P

I

M

E

A

G

G

P

L

A

P

A

G

Y

H

N

R

N

K

A

G

V

C

Q

Q

N

E

L

L

L

F

G

G

K

K

D

D

S

A

A

P

L

V

I

L

A

K

N

D

G

G

Q

L

V

V

V

A

T

T

V

I

E

A

A

T

L

I

G
|
G

T
x
S

G

G

A

A

L

L

K

K

V

V

G

G

A

A

D

E

Y

F

L

I

K

H

R

E

L

W

L

F

P

P

G

Q

A

S

T

K

V

C

Y

Y

I

V

S

S

D

D

P

P

S

T

W
|
W

E

G

N

N

H

H

R

I

A

A

L

I

F

F

E

E

S

G

A

C

G

D

F

I

P

E

V

V

D

G

T

K

Y

Y

P

P

Y

Y

D

D

A

T

A

A

T

T

R

G

G

G

V

I

N

K

F

F

A

D

G

E

M

M

K

I

A

A

K

F

L

F

E

E

S

T

L

L

P

N

A

K

G

D

S

D

I

V

I

L

V

L

L

L

H

H

A

P

C

C

H

H

N

N

P

P

T

T

G

G

A

V

D

D

L

L

S

T

D

R

A

E

Q

Q

W

W

D

D

E

T

V

V

V

L

A

N

A

V

C

I

R

Q

A

E

R

R

G

E

L

L

V

I

P

P

F

F

L

M

D
|
D

M

I

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

G

D

E

G

D

I

M

D

D

A

S

D

D

A

A

V

Y

A

A

V

I

R

R

A

K

F

A

S

V

A

D

S

M

G

G

L

L

Q

P

F

L

F

F

V

V

S

S

S

N

S
|
S

F

F

S
|
S

K
|
K

S

N

F

L

S

S

L

L

Y

Y

G

G

E

E

R
|
R

V

V

G

G

A

G

L

L

S

S

I

V

T

C

A

P

S

T

K

V

E

D

E

E

S

T

G

E

R

R

V

V

L

F

S

G

Q

Q

V

L

K

N

R

S

V

T

I

V

R

R

T

R

N

I

Y

Y

S

S

N

S

P

P

I

S

H

H

G

G

A

R

I

V

V

V

A

D

A

I

V

V

L

M

N

N

S

D

A

A

E

A

L

L

R

H

Q

E

Q

Q

W

W

E

V

D

G

E

E

L

V

A

Y

G

A

M

M

R

R

D

D

R

R

I

I

R

K

A

S

M

M

R

R

T

T

G

K

L

L

V

K

E

S

Q

V

L

L

K

E

A

A

E

K

G

I

V

S

A

G

Q

R

D

N

F

F

S

D

F

Y

V

L

I

T

K

A

Q

Q

R

N

G

G

M

M

F

F

S

S

Y

F

T

T

G

G

L

L

T

T

A

P

A

E

Q

Q

V

V

E

E

K

R

L

L

K

Q

T

S

D

E

F

F

G

G

I

I

Y

Y

A

M

V

I

S

S

T

N

G

S

R
|
R

I

M

C

C

L

V

A

A

A

G

L

L

N

N

S

S

K

S

N

N

I

I

G

D

Y

Y

V

V

A

A

K

N

A

A

I

M

A

V

A

D

V

V

V

L

K

K

binding pyridoxal-5'-phosphate: G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), D211 (= D215), A213 (= A217), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)
binding (2S)-2-hydroxy-3-(4-hydroxyphenyl)propanoic acid: G34 (= G38), W130 (= W134), R374 (= R378)

6zupA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with substrate analog - l-(-)-3-phenyllactic acid (see paper)
47% identity, 99% coverage: 5:401/402 of query aligns to 1:397/398 of 6zupA

query
sites
6zupA

I

M

F

F

A

E

A

R

V

I

E

D

L

Y

A

Y

P

A

R

G

D

D

P

P

I
|
I

L

L

G

G

L

L

N

V

E

E

A

K

F

F

A

A

D

D

T

N

R

N

A

P

Q

D

K

K

V

V

N

N

L

L

G

G

V
x
I

G
|
G

V

I

Y

Y

D

D

D

E

N

S

G

G

K

V

I

M

P

P

L

V

L

L

A

D

A

C

V

V

R

K

A

I

A

A

E

E

K

Q

A

R

R

I

L

A

E

D

A

P

M

I

P

S

P

P

R

R

G

P

Y

Y

Q

L

P

P

I

M

E

A

G

G

P

L

A

P

A

G

Y

H

N

R

N

K

A

G

V

C

Q

Q

N

E

L

L

L

F

G

G

K

K

D

D

S

A

A

P

L

V

I

L

A

K

N

D

G

G

Q

L

V

V

V

A

T

T

V

I

E

A

A

T

L

I

G
|
G

T
x
S

G

G

A

A

L

L

K

K

V

V

G

G

A

A

D

E

Y

F

L

I

K

H

R

E

L

W

L

F

P

P

G

Q

A

S

T

K

V

C

Y

Y

I

V

S

S

D

D

P

P

S

T

W
|
W

E

G

N

N

H

H

R

I

A

A

L

I

F

F

E

E

S

G

A

C

G

D

F

I

P

E

V

V

D

G

T

K

Y

Y

P

P

Y

Y

D

D

A

T

A

A

T

T

R

G

G

G

V

I

N

K

F

F

A

D

G

E

M

M

K

I

A

A

K

F

L

F

E

E

S

T

L

L

P

N

A

K

G

D

S

D

I

V

I

L

V

L

L

L

H

H

A

P

C

C

H

H

N
|
N

P

P

T

T

G

G

A

V

D

D

L

L

S

T

D

R

A

E

Q

Q

W

W

D

D

E

T

V

V

V

L

A

N

A

V

C

I

R

Q

A

E

R

R

G

E

L

L

V

I

P

P

F

F

L

M

D
|
D

M

I

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

G

D

E

G

D

I

M

D

D

A

S

D

D

A

A

V

Y

A

A

V

I

R

R

A

K

F

A

S

V

A

D

S

M

G

G

L

L

Q

P

F

L

F

F

V

V

S

S

S

N

S
|
S

F

F

S
|
S

K
|
K

S

N

F

L

S

S

L

L

Y

Y

G

G

E

E

R
|
R

V

V

G

G

A

G

L

L

S

S

I

V

T

C

A

P

S

T

K

V

E

D

E

E

S

T

G

E

R

R

V

V

L

F

S

G

Q

Q

V

L

K

N

R

S

V

T

I

V

R

R

T

R

N

I

Y

Y

S

S

N

S

P

P

I

S

H

H

G

G

A

R

I

V

V

V

A

D

A

I

V

V

L

M

N

N

S

D

A

A

E

A

L

L

R

H

Q

E

Q

Q

W

W

E

V

D

G

E

E

L

V

A

Y

G

A

M

M

R

R

D

D

R

R

I

I

R

K

A

S

M

M

R

R

T

T

G

K

L

L

V

K

E

S

Q

V

L

L

K

E

A

A

E

K

G

I

V

S

A

G

Q

R

D

N

F

F

S

D

F

Y

V

L

I

T

K

A

Q

Q

R

N

G

G

M

M

F

F

S

S

Y

F

T

T

G

G

L

L

T

T

A

P

A

E

Q

Q

V

V

E

E

K

R

L

L

K

Q

T

S

D

E

F

F

G

G

I

I

Y

Y

A

M

V

I

S

S

T

N

G

S

R
|
R

I

M

C

C

L

V

A

A

A

G

L

L

N

N

S

S

K

S

N

N

I

I

G

D

Y

Y

V

V

A

A

K

N

A

A

I

M

A

V

A

D

V

V

V

L

K

K

binding alpha-hydroxy-beta-phenyl-propionic acid: I13 (= I17), I33 (≠ V37), G34 (= G38), W130 (= W134), K246 (= K250), R374 (= R378)
binding pyridoxal-5'-phosphate: G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), N183 (= N187), D211 (= D215), A213 (= A217), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)

4rkdC Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with aspartic acid (see paper)
47% identity, 99% coverage: 5:401/402 of query aligns to 1:397/398 of 4rkdC

query
sites
4rkdC

I

M

F

F

A

E

A

R

V

I

E

D

L

Y

A

Y

P

A

R

G

D

D

P

P

I

I

L

L

G

G

L

L

N

V

E

E

A

K

F

F

A

A

D

D

T

N

R

N

A

P

Q

D

K

K

V

V

N

N

L

L

G

G

V

I

G

G

V

I

Y

Y

D

D

D

E

N

S

G

G

K

V

I

M

P

P

L

V

L

L

A

D

A

C

V

V

R

K

A

I

A

A

E

E

K

Q

A

R

R

I

L

A

E

D

A

P

M

I

P

S

P

P

R

R

G

P

Y
|
Y

Q

L

P

P

I

M

E

A

G

G

P

L

A

P

A

G

Y

H

N

R

N

K

A

G

V

C

Q

Q

N

E

L

L

L

F

G

G

K

K

D

D

S

A

A

P

L

V

I

L

A

K

N

D

G

G

Q

L

V

V

V

A

T

T

V

I

E

A

A

T

L

I

G
|
G

T
x
S

G

G

A

A

L

L

K

K

V

V

G

G

A

A

D

E

Y

F

L

I

K

H

R

E

L

W

L

F

P

P

G

Q

A

S

T

K

V

C

Y

Y

I

V

S

S

D

D

P

P

S

T

W
|
W

E

G

N

N

H

H

R

I

A

A

L

I

F

F

E

E

S

G

A

C

G

D

F

I

P

E

V

V

D

G

T

K

Y

Y

P

P

Y

Y

D

D

A

T

A

A

T

T

R

G

G

G

V

I

N

K

F

F

A

D

G

E

M

M

K

I

A

A

K

F

L

F

E

E

S

T

L

L

P

N

A

K

G

D

S

D

I

V

I

L

V

L

L

L

H

H

A

P

C

C

H

H

N
|
N

P

P

T

T

G

G

A

V

D

D

L

L

S

T

D

R

A

E

Q

Q

W

W

D

D

E

T

V

V

V

L

A

N

A

V

C

I

R

Q

A

E

R

R

G

E

L

L

V

I

P

P

F

F

L

M

D
|
D

M

I

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

G

D

E

G

D

I

M

D

D

A

S

D

D

A

A

V

Y

A

A

V

I

R

R

A

K

F

A

S

V

A

D

S

M

G

G

L

L

Q

P

F

L

F

F

V

V

S

S

S

N

S
|
S

F

F

S
|
S

K
|
K

S

N

F

L

S

S

L

L

Y

Y

G

G

E

E

R
|
R

V

V

G

G

A

G

L

L

S

S

I

V

T

C

A

P

S

T

K

V

E

D

E

E

S

T

G

E

R

R

V

V

L

F

S

G

Q

Q

V

L

K

N

R

S

V

T

I

V

R
|
R

T

R

N

I

Y

Y

S

S

N

S

P

P

I

S

H

H

G

G

A

R

I

V

V

V

A

D

A

I

V

V

L

M

N

N

S

D

A

A

E

A

L

L

R

H

Q

E

Q

Q

W

W

E

V

D

G

E

E

L

V

A

Y

G

A

M

M

R

R

D

D

R

R

I

I

R

K

A

S

M

M

R

R

T

T

G

K

L

L

V

K

E

S

Q

V

L

L

K

E

A

A

E

K

G

I

V

S

A

G

Q

R

D

N

F

F

S

D

F

Y

V

L

I

T

K

A

Q

Q

R

N

G

G

M

M

F

F

S

S

Y

F

T

T

G

G

L

L

T

T

A

P

A

E

Q

Q

V

V

E

E

K

R

L

L

K

Q

T

S

D

E

F

F

G

G

I

I

Y

Y

A

M

V

I

S

S

T

N

G

S

R

R

I

M

C

C

L

V

A

A

A

G

L

L

N

N

S

S

K

S

N

N

I

I

G

D

Y

Y

V

V

A

A

K

N

A

A

I

M

A

V

A

D

V

V

V

L

K

K

active site: W130 (= W134), D211 (= D215), A213 (= A217), K246 (= K250)
binding oxaloacetate ion: Y65 (= Y69), R280 (= R284)
binding pyridoxal-5'-phosphate: G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), N183 (= N187), D211 (= D215), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)

4rkdA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 cocrystalized with aspartic acid (see paper)
47% identity, 99% coverage: 5:401/402 of query aligns to 1:397/398 of 4rkdA

query
sites
4rkdA

I

M

F

F

A

E

A

R

V

I

E

D

L

Y

A

Y

P

A

R

G

D

D

P

P

I

I

L

L

G

G

L

L

N

V

E

E

A

K

F

F

A

A

D

D

T

N

R

N

A

P

Q

D

K

K

V

V

N

N

L

L

G

G

V

I

G
|
G

V

I

Y

Y

D

D

D

E

N

S

G

G

K

V

I

M

P

P

L

V

L

L

A

D

A

C

V

V

R

K

A

I

A

A

E

E

K

Q

A

R

R

I

L

A

E

D

A

P

M

I

P

S

P

P

R

R

G

P

Y

Y

Q

L

P

P

I

M

E

A

G

G

P

L

A

P

A

G

Y

H

N

R

N

K

A

G

V

C

Q

Q

N

E

L

L

L

F

G

G

K

K

D

D

S

A

A

P

L

V

I

L

A

K

N

D

G

G

Q

L

V

V

V

A

T

T

V

I

E

A

A

T

L

I

G
|
G

T
x
S

G

G

A

A

L

L

K

K

V

V

G

G

A

A

D

E

Y

F

L

I

K

H

R

E

L

W

L

F

P

P

G

Q

A

S

T

K

V

C

Y

Y

I

V

S

S

D

D

P

P

S

T

W
|
W

E

G

N

N

H

H

R

I

A

A

L

I

F

F

E

E

S

G

A

C

G

D

F

I

P

E

V

V

D

G

T

K

Y

Y

P

P

Y

Y

D

D

A

T

A

A

T

T

R

G

G

G

V

I

N

K

F

F

A

D

G

E

M

M

K

I

A

A

K

F

L

F

E

E

S

T

L

L

P

N

A

K

G

D

S

D

I

V

I

L

V

L

L

L

H

H

A

P

C

C

H

H

N

N

P

P

T

T

G

G

A

V

D

D

L

L

S

T

D

R

A

E

Q

Q

W

W

D

D

E

T

V

V

V

L

A

N

A

V

C

I

R

Q

A

E

R

R

G

E

L

L

V

I

P

P

F

F

L

M

D
|
D

M

I

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

G

D

E

G

D

I

M

D

D

A

S

D

D

A

A

V

Y

A

A

V

I

R

R

A

K

F

A

S

V

A

D

S

M

G

G

L

L

Q

P

F

L

F

F

V

V

S

S

S

N

S
|
S

F

F

S
|
S

K
|
K

S

N

F

L

S

S

L

L

Y

Y

G

G

E

E

R
|
R

V

V

G

G

A

G

L

L

S

S

I

V

T

C

A

P

S

T

K

V

E

D

E

E

S

T

G

E

R

R

V

V

L

F

S

G

Q

Q

V

L

K

N

R

S

V

T

I

V

R

R

T

R

N

I

Y

Y

S

S

N

S

P

P

I

S

H

H

G

G

A

R

I

V

V

V

A

D

A

I

V

V

L

M

N

N

S

D

A

A

E

A

L

L

R

H

Q

E

Q

Q

W

W

E

V

D

G

E

E

L

V

A

Y

G

A

M

M

R

R

D

D

R

R

I

I

R

K

A

S

M

M

R

R

T

T

G

K

L

L

V

K

E

S

Q

V

L

L

K

E

A

A

E

K

G

I

V

S

A

G

Q

R

D

N

F

F

S

D

F

Y

V

L

I

T

K

A

Q

Q

R

N

G

G

M

M

F

F

S

S

Y

F

T

T

G

G

L

L

T

T

A

P

A

E

Q

Q

V

V

E

E

K

R

L

L

K

Q

T

S

D

E

F

F

G

G

I

I

Y

Y

A

M

V

I

S

S

T

N

G

S

R

R

I

M

C

C

L

V

A

A

A

G

L

L

N

N

S

S

K

S

N

N

I

I

G

D

Y

Y

V

V

A

A

K

N

A

A

I

M

A

V

A

D

V

V

V

L

K

K

active site: W130 (= W134), D211 (= D215), A213 (= A217), K246 (= K250)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethylene)-amino]-succinic acid: G34 (= G38), G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), D211 (= D215), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)
binding pyridoxal-5'-phosphate: G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), D211 (= D215), A213 (= A217), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)

4rkcA Psychrophilic aromatic amino acids aminotransferase from psychrobacter sp. B6 (see paper)
47% identity, 99% coverage: 5:401/402 of query aligns to 1:397/398 of 4rkcA

query
sites
4rkcA

I

M

F

F

A

E

A

R

V

I

E

D

L

Y

A

Y

P

A

R

G

D

D

P

P

I

I

L

L

G

G

L

L

N

V

E

E

A

K

F

F

A

A

D

D

T

N

R

N

A

P

Q

D

K

K

V

V

N

N

L

L

G

G

V

I

G

G

V

I

Y

Y

D

D

D

E

N

S

G

G

K

V

I

M

P

P

L

V

L

L

A

D

A

C

V

V

R

K

A

I

A

A

E

E

K

Q

A

R

R

I

L

A

E

D

A

P

M

I

P

S

P

P

R

R

G

P

Y

Y

Q

L

P

P

I

M

E

A

G

G

P

L

A

P

A

G

Y

H

N

R

N

K

A

G

V

C

Q

Q

N

E

L

L

L

F

G

G

K

K

D

D

S

A

A

P

L

V

I

L

A

K

N

D

G

G

Q

L

V

V

V

A

T

T

V

I

E

A

A

T

L

I

G
|
G

T
x
S

G

G

A

A

L

L

K

K

V

V

G

G

A

A

D

E

Y

F

L

I

K

H

R

E

L

W

L

F

P

P

G

Q

A

S

T

K

V

C

Y

Y

I

V

S

S

D

D

P

P

S

T

W
|
W

E

G

N

N

H

H

R

I

A

A

L

I

F

F

E

E

S

G

A

C

G

D

F

I

P

E

V

V

D

G

T

K

Y

Y

P

P

Y

Y

D

D

A

T

A

A

T

T

R

G

G

G

V

I

N

K

F

F

A

D

G

E

M

M

K

I

A

A

K

F

L

F

E

E

S

T

L

L

P

N

A

K

G

D

S

D

I

V

I

L

V

L

L

L

H

H

A

P

C

C

H

H

N

N

P

P

T

T

G

G

A

V

D

D

L

L

S

T

D

R

A

E

Q

Q

W

W

D

D

E

T

V

V

V

L

A

N

A

V

C

I

R

Q

A

E

R

R

G

E

L

L

V

I

P

P

F

F

L

M

D
|
D

M

I

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

G

D

E

G

D

I

M

D

D

A

S

D

D

A

A

V

Y

A

A

V

I

R

R

A

K

F

A

S

V

A

D

S

M

G

G

L

L

Q

P

F

L

F

F

V

V

S

S

S

N

S
|
S

F

F

S
|
S

K
|
K

S

N

F

L

S

S

L

L

Y

Y

G

G

E

E

R
|
R

V

V

G

G

A

G

L

L

S

S

I

V

T

C

A

P

S

T

K

V

E

D

E

E

S

T

G

E

R

R

V

V

L

F

S

G

Q

Q

V

L

K

N

R

S

V

T

I

V

R

R

T

R

N

I

Y

Y

S

S

N

S

P

P

I

S

H

H

G

G

A

R

I

V

V

V

A

D

A

I

V

V

L

M

N

N

S

D

A

A

E

A

L

L

R

H

Q

E

Q

Q

W

W

E

V

D

G

E

E

L

V

A

Y

G

A

M

M

R

R

D

D

R

R

I

I

R

K

A

S

M

M

R

R

T

T

G

K

L

L

V

K

E

S

Q

V

L

L

K

E

A

A

E

K

G

I

V

S

A

G

Q

R

D

N

F

F

S

D

F

Y

V

L

I

T

K

A

Q

Q

R

N

G

G

M

M

F

F

S

S

Y

F

T

T

G

G

L

L

T

T

A

P

A

E

Q

Q

V

V

E

E

K

R

L

L

K

Q

T

S

D

E

F

F

G

G

I

I

Y

Y

A

M

V

I

S

S

T

N

G

S

R

R

I

M

C

C

L

V

A

A

A

G

L

L

N

N

S

S

K

S

N

N

I

I

G

D

Y

Y

V

V

A

A

K

N

A

A

I

M

A

V

A

D

V

V

V

L

K

K

active site: W130 (= W134), D211 (= D215), A213 (= A217), K246 (= K250)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G102 (= G106), G103 (= G107), S104 (≠ T108), W130 (= W134), D211 (= D215), A213 (= A217), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)

5vwrA E.Coli aspartate aminotransferase-(1r,3s,4s)-3-amino-4- fluorocyclopentane-1-carboxylic acid (fcp)-alpha-ketoglutarate (see paper)
45% identity, 99% coverage: 5:400/402 of query aligns to 1:396/396 of 5vwrA

query
sites
5vwrA

I

M

F

F

A

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

A

R

A

A

D

D

T

E

R

R

A

P

Q

G

K

K

V

I

N

N

L

L

G

G

V

I

G
|
G

V

V

Y

Y

Y

K

D

D

D

E

N

T

G

G

K

K

I

T

P

P

L

V

L

L

A

T

A

S

V

V

R

K

A

K

A

A

E

E

K

Q

A

Y

R

L

L

L

E

E

A

N

M

E

P

T

R

K

G

N

Y

Y

Q

L

P

G

I

I

E

D

G

G

P

I

A

P

A

E

Y

F

N

G

N

R

A

C

V

T

Q

Q

N

E

L

L

L

F

G

G

K

K

D

G

S

S

A

A

L

L

I

I

A

N

N

D

G

K

Q

R

V

A

V

R

T

T

V

A

E

Q

A

T

L

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

V

V

G

A

A

A

D

D

Y

F

L

L

K

A

R

K

L

N

L

T

P

S

G

V

A

K

T

R

V

V

Y

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H

H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

D

R

T

E

Y

Y

P

A

Y

Y

D

D

A

A

A

E

T

N

R

H

G

T

V

L

N

D

F

F

A

D

G

A

M

L

K

I

A

N

K

S

L

L

E

N

S

E

L

A

P

Q

A

A

G

G

S

D

I

V

V

L

L

F

H

H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

I

D

D

L

P

S

T

D

L

A

E

Q

Q

W

W

D

Q

E

T

V

L

V

A

A

Q

A

L

C

S

R

V

A

E

R

K

G

G

L

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

A

D

R

G

G

I

L

D

E

A

E

D

D

A

A

V

E

A

G

V

L

R

R

A

A

F

F

S

A

A

A

S

M

G

H

L

K

Q

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

I

V

T

A

A

A

S

D

K

S

E

E

E

T

S

V

G

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R

R

T

A

N

N

Y

Y

S

S

N

N

P

P

I

A

H

H

G

G

G

A

A

S

I

V

V

V

A

A

A

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

Q

I

W

W

E

E

D

Q

E

E

L

L

A

T

G

D

M

M

R

R

D

Q

R

R

I

I

R

Q

A

R

M

M

R

R

T

Q

G

L

L

F

V

V

E

N

Q

T

L

L

K

Q

A

E

E

K

G

G

V

A

A

N

Q

R

D

D

F

F

S

S

F

F

V

I

I

I

K

K

Q

Q

R

N

G

G

M

M

F

F

S

S

Y

F

T

S

G

G

L

L

T

T

A

K

A

E

Q

Q

V

V

E

L

K

R

L

L

K

R

T

E

D

E

F

F

G

G

I

V

Y

Y

A

A

V

V

S

A

T

S

G

G

R
|
R

I

V

C

N

L

V

A

A

A

G

L

M

N

T

S

P

K

D

N

N

I

M

G

A

Y

P

V

L

A

C

K

E

A

A

I

I

A

V

A

A

V

V

V

L

active site: W130 (= W134), D211 (= D215), A213 (= A217), K246 (= K250)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-glutamic acid: I13 (= I17), G34 (= G38), G102 (= G106), G103 (= G107), T104 (= T108), W130 (= W134), N183 (= N187), D211 (= D215), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258), R374 (= R378)

5t4lA Plp and gaba trigger gabr-mediated transcription regulation in bacillus subsidies via external aldimine formation (see paper)
45% identity, 99% coverage: 5:400/402 of query aligns to 1:396/396 of 5t4lA

query
sites
5t4lA

I

M

F

F

A

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I

I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

A

R

A

A

D

D

T

E

R

R

A

P

Q

G

K

K

V

I

N

N

L

L

G

G

V

I

G

G

V

V

Y

Y

Y

K

D

D

D

E

N

T

G

G

K

K

I

T

P

P

L

V

L

L

A

T

A

S

V

V

R

K

A

K

A

A

E

E

K

Q

A

Y

R

L

L

L

E

E

A

N

M

E

P

T

R

K

G

N

Y

Y

Q

L

P

G

I

I

E

D

G

G

P

I

A

P

A

E

Y

F

N

G

N

R

A

C

V

T

Q

Q

N

E

L

L

L

F

G

G

K

K

D

G

S

S

A

A

L

L

I

I

A

N

N

D

G

K

Q

R

V

A

V

R

T

T

V

A

E

Q

A

T

L

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

V

V

G

A

A

A

D

D

Y

F

L

L

K

A

R

K

L

N

L

T

P

S

G

V

A

K

T

R

V

V

Y

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H

H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

D

R

T

E

Y

Y

P

A

Y

Y

D

D

A

A

A

E

T

N

R

H

G

T

V

L

N

D

F

F

A

D

G

A

M

L

K

I

A

N

K

S

L

L

E

N

S

E

L

A

P

Q

A

A

G

G

S

D

I

V

V

L

L

F

H

H

A

G

C

C

H

H

N

N

P

P

T

T

G

G

A

I

D

D

L

P

S

T

D

L

A

E

Q

Q

W

W

D

Q

E

T

V

L

V

A

A

Q

A

L

C

S

R

V

A

E

R

K

G

G

L

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

A

D

R

G

G

I

L

D

E

A

E

D

D

A

A

V

E

A

G

V

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R

R

A

A

F

F

S

A

A

A

S

M

G

H

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K

Q

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

I

V

T

A

A

A

S

D

K

S

E

E

E

T

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D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R

R

T

A

N

N

Y

Y

S

S

N

N

P

P

I

A

H

H

G

G

G

A

A

S

I

V

V

V

A

A

A

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

Q

I

W

W

E

E

D

Q

E

E

L

L

A

T

G

D

M

M

R

R

D

Q

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R

I

I

R

Q

A

R

M

M

R

R

T

Q

G

L

L

F

V

V

E

N

Q

T

L

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K

Q

A

E

E

K

G

G

V

A

A

N

Q

R

D

D

F

F

S

S

F

F

V

I

I

I

K

K

Q

Q

R

N

G

G

M

M

F

F

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Y

F

T

S

G

G

L

L

T

T

A

K

A

E

Q

Q

V

V

E

L

K

R

L

L

K

R

T

E

D

E

F

F

G

G

I

V

Y

Y

A

A

V

V

S

A

T

S

G

G

R

R

I

V

C

N

L

V

A

A

A

G

L

M

N

T

S

P

K

D

N

N

I

M

G

A

Y

P

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L

A

C

K

E

A

A

I

I

A

V

A

A

V

V

V

L

active site: W130 (= W134), D211 (= D215), A213 (= A217), K246 (= K250)
binding (4R)-4-amino-6-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}hexanoic acid: G102 (= G106), G103 (= G107), T104 (= T108), W130 (= W134), D211 (= D215), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258)

3qpgA Crystal structures of escherichia coli aspartate aminotransferase reconstituted with 1-deaza-pyridoxal 5'-phosphate: internal aldimine and stable l-aspartate external aldimine (see paper)
45% identity, 99% coverage: 5:400/402 of query aligns to 1:396/396 of 3qpgA

query
sites
3qpgA

I

M

F

F

A

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

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I
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I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

A

R

A

A

D

D

T

E

R

R

A

P

Q

G

K

K

V

I

N

N

L

L

G

G

V

I

G
|
G

V

V

Y

Y

Y

K

D

D

D

E

N

T

G

G

K

K

I

T

P

P

L

V

L

L

A

T

A

S

V

V

R

K

A

K

A

A

E

E

K

Q

A

Y

R

L

L

L

E

E

A

N

M

E

P

T

R

K

G

N

Y

Y

Q

L

P

G

I

I

E

D

G

G

P

I

A

P

A

E

Y

F

N

G

N

R

A

C

V

T

Q

Q

N

E

L

L

L

F

G

G

K

K

D

G

S

S

A

A

L

L

I

I

A

N

N

D

G

K

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R

V

A

V

R

T

T

V

A

E

Q

A

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L

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

V

V

G

A

A

A

D

D

Y

F

L

L

K

A

R

K

L

N

L

T

P

S

G

V

A

K

T

R

V

V

Y

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H

H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

D

R

T

E

Y

Y

P

A

Y

Y

D

D

A

A

A

E

T

N

R

H

G

T

V

L

N

D

F

F

A

D

G

A

M

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I

A

N

K

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L

L

E

N

S

E

L

A

P

Q

A

A

G

G

S

D

I

V

V

L

L

F

H

H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

I

D

D

L

P

S

T

D

L

A

E

Q

Q

W

W

D

Q

E

T

V

L

V

A

A

Q

A

L

C

S

R

V

A

E

R

K

G

G

L

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

A

A

D

R

G

G

I

L

D

E

A

E

D

D

A

A

V

E

A

G

V

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R

R

A

A

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F

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A

A

A

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M

G

H

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K

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E

F

L

F

I

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S

A

S

S

S
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S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

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T

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L

I

V

T

A

A

A

S

D

K

S

E

E

E

T

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R

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A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R

R

T

A

N

N

Y

Y

S

S

N

N

P

P

I

A

H

H

G

G

G

A

A

S

I

V

V

V

A

A

A

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

Q

I

W

W

E

E

D

Q

E

E

L

L

A

T

G

D

M

M

R

R

D

Q

R

R

I

I

R

Q

A

R

M

M

R

R

T

Q

G

L

L

F

V

V

E

N

Q

T

L

L

K

Q

A

E

E

K

G

G

V

A

A

N

Q

R

D

D

F

F

S

S

F

F

V

I

I

I

K

K

Q

Q

R

N

G

G

M

M

F

F

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S

Y

F

T

S

G

G

L

L

T

T

A

K

A

E

Q

Q

V

V

E

L

K

R

L

L

K

R

T

E

D

E

F

F

G

G

I

V

Y

Y

A

A

V

V

S

A

T

S

G

G

R
|
R

I

V

C

N

L

V

A

A

A

G

L

M

N

T

S

P

K

D

N

N

I

M

G

A

Y

P

V

L

A

C

K

E

A

A

I

I

A

V

A

A

V

V

V

L

active site: W130 (= W134), D211 (= D215), A213 (= A217), K246 (= K250)
binding (E)-N-{2-hydroxy-3-methyl-6-[(phosphonooxy)methyl]benzylidene}-L-aspartic acid: I13 (= I17), G34 (= G38), G102 (= G106), G103 (= G107), T104 (= T108), W130 (= W134), N183 (= N187), D211 (= D215), Y214 (= Y218), S243 (= S247), S245 (= S249), K246 (= K250), R254 (= R258), R374 (= R378)

3pa9A Mechanism of inactivation of e. Coli aspartate aminotransferase by (s)-4-amino-4,5-dihydro-2-furancarboxylic acid (s-adfa) ph 7.5 (see paper)
45% identity, 99% coverage: 5:400/402 of query aligns to 1:396/396 of 3pa9A

query
sites
3pa9A

I

M

F

F

A

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

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D

P

P

I

I

L

L

G

G

L

L

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A

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A

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F

A
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