SitesBLAST
Comparing WP_011766303.1 NCBI__GCF_000061505.1:WP_011766303.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1drvA Escherichia coli dhpr/acnadh complex (see paper)
59% identity, 99% coverage: 5:269/269 of query aligns to 3:268/270 of 1drvA
- active site: H156 (= H157), K160 (= K161)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G11), G12 (= G14), R13 (= R15), M14 (= M16), E35 (≠ D37), F76 (= F77), T77 (= T78), R78 (= R79), G81 (= G82), G99 (= G100), A124 (≠ P125), F126 (≠ M127), R237 (= R238)
1druA Escherichia coli dhpr/nadh complex (see paper)
59% identity, 99% coverage: 5:269/269 of query aligns to 3:268/270 of 1druA
- active site: H156 (= H157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G9 (= G11), G12 (= G14), R13 (= R15), M14 (= M16), E35 (≠ D37), R36 (= R38), F76 (= F77), T77 (= T78), R78 (= R79), G81 (= G82), G99 (= G100), T100 (= T101), T101 (= T102), A124 (≠ P125), N125 (= N126), F126 (≠ M127), F240 (≠ Y241)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
59% identity, 99% coverage: 5:269/269 of query aligns to 3:268/270 of 1arzA
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
59% identity, 99% coverage: 5:269/269 of query aligns to 2:267/269 of 1arzB
- active site: H155 (= H157), K159 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G11), G10 (≠ S13), G11 (= G14), R12 (= R15), M13 (= M16), E34 (≠ D37), F75 (= F77), T76 (= T78), R77 (= R79), G80 (= G82), H84 (= H86), G98 (= G100), T100 (= T102), A123 (≠ P125), N124 (= N126), F125 (≠ M127), F239 (≠ Y241)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T102), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168), F239 (≠ Y241)
1drwA Escherichia coli dhpr/nhdh complex (see paper)
59% identity, 99% coverage: 5:269/269 of query aligns to 5:270/272 of 1drwA
- active site: H158 (= H157), K162 (= K161)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G11), G14 (= G14), R15 (= R15), M16 (= M16), E37 (≠ D37), R38 (= R38), F78 (= F77), T79 (= T78), R80 (= R79), G101 (= G100), T102 (= T101), T103 (= T102), A126 (≠ P125), N127 (= N126), F128 (≠ M127), F242 (≠ Y241)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
59% identity, 99% coverage: 5:269/269 of query aligns to 5:270/272 of 1dihA
- active site: H158 (= H157), K162 (= K161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), G14 (= G14), R15 (= R15), M16 (= M16), R38 (= R38), F78 (= F77), T79 (= T78), R80 (= R79), G83 (= G82), G101 (= G100), T103 (= T102), N127 (= N126), F128 (≠ M127), R239 (= R238), F242 (≠ Y241)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
59% identity, 99% coverage: 5:269/269 of query aligns to 6:271/273 of P04036
- G12 (= G11) binding NADP(+)
- GRM 15:17 (= GRM 14:16) binding NAD(+)
- RM 16:17 (= RM 15:16) binding NADP(+)
- E38 (≠ D37) binding NAD(+)
- R39 (= R38) binding NADP(+)
- TR 80:81 (= TR 78:79) binding NAD(+)
- GTT 102:104 (= GTT 100:102) binding NAD(+); binding NADP(+)
- AANF 126:129 (≠ APNM 124:127) binding NAD(+)
- F129 (≠ M127) binding NADP(+)
- H159 (= H157) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K161) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R238) binding NADP(+)
- F243 (≠ Y241) binding NAD(+)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
58% identity, 98% coverage: 6:269/269 of query aligns to 3:268/268 of 4ywjA
- active site: H156 (= H157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), R12 (= R15), M13 (= M16), D35 (= D37), R36 (= R38), F76 (= F77), T77 (= T78), V81 (≠ G82), G99 (= G100), T101 (= T102), A124 (≠ P125), N125 (= N126), F126 (≠ M127), R237 (= R238), F240 (≠ Y241)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
50% identity, 99% coverage: 5:269/269 of query aligns to 2:267/269 of 5tejB
- active site: H155 (= H157), K159 (= K161)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T102), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168)
- binding nicotinamide-adenine-dinucleotide: G8 (= G11), G11 (= G14), R12 (= R15), M13 (= M16), E34 (≠ D37), R35 (= R38), F75 (= F77), T76 (= T78), S80 (≠ G82), G98 (= G100), T100 (= T102), P123 (= P125), N124 (= N126), Y125 (≠ M127), F239 (≠ Y241)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
50% identity, 99% coverage: 5:269/269 of query aligns to 2:267/269 of 5tejA
- active site: H155 (= H157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G11), G11 (= G14), R12 (= R15), M13 (= M16), E34 (≠ D37), R35 (= R38), F75 (= F77), T76 (= T78), S80 (≠ G82), G98 (= G100), T100 (= T102), P123 (= P125)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
50% identity, 98% coverage: 5:268/269 of query aligns to 2:266/266 of 5temA
- active site: H155 (= H157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G11), G11 (= G14), R12 (= R15), M13 (= M16), E34 (≠ D37), R35 (= R38), F75 (= F77), T76 (= T78), S80 (≠ G82), G98 (= G100), T100 (= T102), P123 (= P125), N124 (= N126), Y125 (≠ M127), F239 (≠ Y241)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T102), P123 (= P125), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
41% identity, 99% coverage: 4:269/269 of query aligns to 1:267/267 of 3ijpB
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
41% identity, 99% coverage: 4:268/269 of query aligns to 1:266/266 of 3ijpA
- active site: H155 (= H157), K159 (= K161)
- binding sodium ion: I21 (≠ A24), Q22 (≠ L25), R24 (≠ D27), V27 (= V30)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), N10 (≠ S13), G11 (= G14), R12 (= R15), M13 (= M16), R35 (= R38), F75 (= F77), S76 (≠ T78), Q77 (≠ R79), A80 (≠ G82), G98 (= G100), T100 (= T102), G123 (≠ P125), N124 (= N126), M125 (= M127), F239 (≠ Y241)
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
30% identity, 97% coverage: 5:266/269 of query aligns to 1:212/216 of Q9X1K8
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
30% identity, 97% coverage: 5:266/269 of query aligns to 6:217/218 of 1vm6B
- active site: H132 (= H157), K136 (= K161)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), S14 (= S13), G15 (= G14), R16 (= R15), M17 (= M16), D37 (≠ A35), V38 (≠ A55), F53 (= F77), S54 (≠ T78), S55 (≠ R79), E57 (= E81), A58 (≠ G82), G76 (= G100), T78 (= T102), Y101 (≠ P125), N102 (= N126), F103 (≠ M127), F192 (≠ Y241)
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 89% coverage: 5:244/269 of query aligns to 3:243/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ S13), G12 (= G14), K13 (≠ R15), M14 (= M16), D35 (= D37), H36 (≠ R38), K37 (≠ A39), L76 (≠ F77), T77 (= T78), G99 (= G100), T100 (= T101), T101 (= T102), P126 (= P125), N127 (= N126), F128 (≠ M127)
- binding pyridine-2,6-dicarboxylic acid: P126 (= P125), H155 (= H157), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168), A215 (≠ G216)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 89% coverage: 5:244/269 of query aligns to 3:243/265 of 5z2eA
1p9lA Structure of m. Tuberculosis dihydrodipicolinate reductase in complex with nadh and 2,6 pdc (see paper)
30% identity, 99% coverage: 5:269/269 of query aligns to 1:245/245 of 1p9lA
- active site: H132 (= H157), K136 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G7 (= G11), G10 (= G14), K11 (≠ R15), V12 (≠ M16), D33 (= D37), A34 (= A39), F52 (= F77), T53 (= T78), V57 (≠ G82), G75 (= G100), T77 (= T102), P103 (= P125), N104 (= N126), F105 (≠ M127), F217 (≠ Y241)
- binding pyridine-2,6-dicarboxylic acid: H133 (= H158), K136 (= K161), S141 (= S166), G142 (= G167), T143 (= T168), A192 (≠ G216)
1c3vA Dihydrodipicolinate reductase from mycobacterium tuberculosis complexed with NADPH and pdc (see paper)
30% identity, 99% coverage: 5:269/269 of query aligns to 1:245/245 of 1c3vA
- active site: H132 (= H157), K136 (= K161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K9 (≠ S13), G10 (= G14), K11 (≠ R15), V12 (≠ M16), D33 (= D37), A34 (= A39), F52 (= F77), T53 (= T78), V57 (≠ G82), G75 (= G100), T77 (= T102), P103 (= P125), N104 (= N126), F217 (≠ Y241)
- binding pyridine-2,6-dicarboxylic acid: T77 (= T102), N104 (= N126), K136 (= K161), S141 (= S166), G142 (= G167), T143 (= T168), A192 (≠ G216)
P9WP23 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 99% coverage: 5:269/269 of query aligns to 1:245/245 of P9WP23
- K9 (≠ S13) mutation to A: Increases the nucleotide specificity from 6:1 for the wild-type enzyme to 34:1, due to a 4-fold decrease in NADPH affinity while the affinity for NADH remains nearly unchanged.
- K11 (≠ R15) mutation to A: 2.8-fold increase in catalytic activity with NADH as substrate, while the affinity for NADH is essentially unaffected. 70-fold decrease in affinity for NADPH, causing the nucleotide specificity to increase from 6:1 for the wild-type enzyme to 187:1.
- KV 11:12 (≠ RM 15:16) binding NAD(+); binding NADP(+)
- D33 (= D37) binding NAD(+)
- GTT 75:77 (= GTT 100:102) binding NAD(+); binding NADP(+)
- APNF 102:105 (≠ APNM 124:127) binding NAD(+); binding NADP(+)
- K136 (= K161) binding NAD(+); binding NADP(+)
Query Sequence
>WP_011766303.1 NCBI__GCF_000061505.1:WP_011766303.1
MTTPVRVAIAGASGRMGRMLIEAALKDEEVVLAAAFDRAGSPFIGRDAGEFAGVASGVAI
TDDVHAAVAAADCVIDFTRPEGTLAHLAIARELGKAMVIGTTGFEAAGKAAIADAAQAIP
VVFAPNMAVGVNAVFRLLEVAARILDEGYDVEVIEAHHRFKVDAPSGTALRMGEVVAREL
GRDLETCAIYGREGVTGERKAETIGFSTIRGGDVVGDHTVLFAGIGERIEITHKSGSRMP
YALGSMRAARFLRGRSNGLFDMQDVLGLR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory